Supp F2 10 01 2007 2
1
0 0.2 0.4 0.6 0.8 1 1.2 0 1 2 3 4 5 6 7 8 9 10 11 12 D ay OD 600 10403S sigC ∆ sigH ∆ sigL ∆ rsbV ∆ 0 0.2 0.4 0.6 0.8 1 1.2 0 1 2 3 4 5 6 7 8 9 10 11 12 D ay OD 600 10403S ctsR ∆ lisR ∆ lmo1060 ∆ lmo1172 ∆ 7.0 7.5 8.0 8.5 9.0 9.5 0 3 6 9 12 D ay Log C FU /m L 7.0 7.5 8.0 8.5 9.0 9.5 0 3 6 9 12 D ay Log C FU /m L (A) (B) (C) (D) SUPPLEMENTAL FIGURE S2. Growth in BHI over 12 days at 4°C of L. monocytogenes strain 10403S and selected null mutant strains, using a starting inoculum of 10 8 CFU/ml. Data are only shown for the L. monocytogenes parent strain (10403S) and those mutant strains that showed significantly reduced growth between day 0 and 12 (see Fig. 1). Bacterial numbers in log CFU/ml were determined on day 0, 3, 6, 9, and 12 and are shown in (A) for the parent strain, ΔsigC, ΔsigH, ΔsigL, and ΔrsbV strains and in (B) for the parent strain (same data as shown in A), ΔctsR, ΔlisR, Δlmo1060, and Δlmo1172 strains; the data are shown in two graphs to maintain readability of symbols. OD 600 measurements were taken every day for 12 days and are shown in (C) for the parent strain, ΔsigC, ΔsigH, ΔsigL, and ΔrsbV, and in (D) for the parent strain (same data as shown in C), ΔctsR, ΔlisR, Δlmo1060, and Δlmo1172. Data represent a single experiment.
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Transcript of Supp F2 10 01 2007 2
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(A) (B)
(C) (D)
SUPPLEMENTAL FIGURE S2. Growth in BHI over 12 days at 4°C of L. monocytogenes strain 10403S and selected null mutant strains, using a starting inoculum of 108 CFU/ml. Data are only shown for the L. monocytogenes parent strain (10403S) and those mutant strains that showed significantly reduced growth between day 0 and 12 (see Fig. 1). Bacterial numbers in log CFU/ml were determined on day 0, 3, 6, 9, and 12 and are shown in (A) for the parent strain, ΔsigC, ΔsigH, ΔsigL, and ΔrsbV strains and in (B) for the parent strain (same data as shown in A), ΔctsR, ΔlisR, Δlmo1060, and Δlmo1172 strains; the data are shown in two graphs to maintain readability of symbols. OD600 measurements were taken every day for 12 days and are shown in (C) for the parent strain, ΔsigC, ΔsigH, ΔsigL, and ΔrsbV, and in (D) for the parent strain (same data as shown in C), ΔctsR, ΔlisR, Δlmo1060, and Δlmo1172. Data represent a single experiment.
