Suicide Ideation in the depression group (NBB)

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Suicide Ideation in the depression group (NBB) A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting to detailed planning, role playing, self-harm and attempts GABRD N oS uicideIdeation S uicideIdeation 0.00 0.05 0.10 0.15 0.20 0.25 GABBR2 N oS uicideIdeation S uicideIdeation 0.8 1.0 1.2 1.4 1.6 G luR1 N oS uicideIdeation S uicideIdeation 0.14 0.16 0.18 0.20 0.22 0.24 * * * * DLPFC was also checked, however, none of the target genes were unchanged. Anterior Cingulate Cortex Juan Zhao and Dick Swaab, submitted

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Anterior Cingulate Cortex. Suicide Ideation in the depression group (NBB). *. **. *. A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting to detailed planning, role playing, self-harm and attempts. - PowerPoint PPT Presentation

Transcript of Suicide Ideation in the depression group (NBB)

Page 1: Suicide Ideation in the depression group (NBB)

Suicide Ideation in the depression group (NBB)A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting to detailed planning, role playing, self-harm and attempts

GABRD

NoSuicideIdeation SuicideIdeation0.00

0.05

0.10

0.15

0.20

0.25

GABBR2

NoSuicideIdeation SuicideIdeation0.8

1.0

1.2

1.4

1.6

GluR1

NoSuicideIdeation SuicideIdeation0.14

0.16

0.18

0.20

0.22

0.24* * **

DLPFC was also checked, however, none of the target genes were unchanged.

Anterior Cingulate Cortex

Juan Zhao and Dick Swaab, submitted

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Microglia isolation procedure

• Mechanic & enzymatic dissociation

• Cell separation: Percoll gradient

• Take off myelin

• Collect cells from interface

• Stain cells with labeled antibodies:o CD11b - PEo CD45 - FITCo CD15 - APC

• Selection using magnetic CD11b beads

Melief et al. Glia 2012

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Isolation and phenotyping of pure primary human microglia

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Peripheral inflammation impacts on microglia activation

Melief et al. Glia 2012

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The Netherlands Brain Bank for Psychiatry

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Phenotype & Characterize human post mortem microglia

Jeroen Melief, thesis 2014

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