Study of 7chemotypes of thymes

Thymes: Linalool,thuyanol,carvacrol, thymol, lemony,wild,geraniol, borneol.

POLYPHENOLS

Coumarins

• Aesculus hippocastanum

Ethyl acetate /chloroform 60/40 v/v on silicagel

Flavonoids

Ethyl acetate /chloroform 40:60 v/v

on silicagel

Anthocyanins

• Malva sylvestris

Ethyl acetate MEK formic acid hydrochloric acid 65/10/6/9 v/v on silicagel

Oplc semi preparative mode

ability to perform high performance separations of difficult samples with little to no sample preparation

ability to visually check directly on the chromatographic phase that all compounds have actually been eluted

abiity for rapid scale-up on the same instrument

ability collect several tenth of mg of pure compounds

ability to select either on-line or off-line injection depending on the sample matrix

Oplc semipreparative mode 2

• Personal works– isolation of– cannabidiol from hemp– strychnine, brucine from strychnos– lupanine from lupinus– morphine, codeine, thebaine, papaverine,

noscapine from opium