Strawberry lab dna extraction 2016
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Transcript of Strawberry lab dna extraction 2016
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Strawberries
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• DNA is present in the cells of all living organisms.
• The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology.
• The scientist must be able to separate DNA from the unwanted substances of the cell gently enough so that the DNA does not denature (break up).
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• Each cell contains 9 feet of DNA
• In an average meal, you EAT approximately 55,000,000 cells
• This is equal to approximately 93,205 miles of DNA.
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• DNA is found in the nucleus of all eukaryotic cells
• Most cells have the diploid 2nchromosome number
• Many plants are polyploid (contain several sets of chromosomes)
• Strawberries are octaploid 8n
• This means there is more DNA in strawberries
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• DNA is enclosed in a nuclear and a cell membrane made of phospholipids
• DNA is also coiled around proteins
• Both the phospholipid layer and the proteins must be removed to see DNA
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Plant Cell Wall: cellulose and protein matrix
Cell membrane: lipids and protein
Plant Cell
Cytoplasm: proteins etc
Nucleus: DNA & protein
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• Cell membrane
• Cytoplasm
• Nuclear membrane
• Proteins
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Be accurate in recording
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• Place the strawberry in a resealable plastic bagand remove the air
• Squash the berry with your hand to begin the process
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• Cell membranes are made of phospholipids
• Phospholipids won’t dissolve in water
• What do you use in your home to remove oils from your hands or your dishes?
• Soap or Detergent!
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• Soap gathers between oil and water capturing the oil in a bubble called a micelle.
• When you wash your hands, the soap carries the oils away.
• Dawn dishwashing works BEST to remove phospholipid membranes
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• By adding a small amount of table salt (NaCl) to the soap solution, the solution can punch holes in the nuclear and cell membranes
• The soapy solutionalso helps removes proteins
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• DNA is tightly coiled around proteins called histones
• Proteins must be removed to see DNA
• Enzymes, like the ones found in meat tenderizer, can remove protein
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Mashing the berry disrupts the cells & membrane
What is in the bag?
1. Clumps of whole cells and nuclei 2. Cell Membranes: lipids and protein 3. Organelles made of protein4. Plant Cell Wall: cellulose and
protein5. DNA bound to protein
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• Clumps of cellular debris get stuck in the filter.
• Small molecules flow thru.
How do we get the
DNA to separate from
the pink liquid?
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• The contents of the cell(organelles, proteins, etc.) must be separated from the DNA
• The larger cell parts can be removed by filtering the solid from the liquid
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• To see DNA, it must be extracted or “spooled”from the remaining liquid you filtered
• DNA dissolves in water, but NOT in alcohol
• Adding COLD ALCOHOL will cause DNA to precipitate (separate out) from the liquid filtrate
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• The DNA will appear as a white precipitate once the alcohol is added
• HOLD THE TUBE by the TOP, not the bottom so the DNA strands won’t fragment from the heat of your hands!
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• After alcohol is added
• DNA separates
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• DNA is sticky and will adhere to other surfaces
• A bamboo skewer can be used to spool (remove) the DNA by using a turning motion.
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