Amines Amines are organic compounds containing a nitrogen ...
Strata SPE Sample Preparation Productsphx.phenomenex.com/lib/6180_I.pdfSCX Strong cation exchange....
Transcript of Strata SPE Sample Preparation Productsphx.phenomenex.com/lib/6180_I.pdfSCX Strong cation exchange....
Strata SPE Sample Preparation Products
• CleanExtracts• HigherRecovery• BetterReproducibility
Guaranteed Performance OurStrataSPEproductlineembodiesqualityandperformance.Itsextensivequalitycontrolprocedure
providestrouble-freesolidphaseextractionmethods.AllStrataSPEphasesandformatsaretheresultof
asophisticatedmanufacturingprocess-eliminatingvariability.
EveryproductundergoescomprehensiveQCtestingtoensurereproducibleproductmanufacturing
• Consistentflowcharacteristics
• Consistentphaseselectivity
• Inertsorbentandmaterials
Cross Reference
©2008Phenomenex,Inc.Allrightsreserved.
If Strata SPE or Sepra bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, return the product with comparative data within 45 days for a FULL REFUND.
Phenomenex Strata
Waters Sep-Pak
Varian Bond Elut
Supelco Discovery UCT
JT BAKER Bakerbond
IST ISOLUTE
Macherey-Nagel Chromabond
Reversed PhaseC18-E tC18 C18 DSC-18 C18 Octadecyl C18(EC) C18ecC18-U PolarPlus
OctadecylLightloadC18 C18
C18PAHC18-T C18 DStC-18LtC8 C8 C8 DSC-8 C8 Octyl C8(EC) C8Phenyl(PH) PH DSC-PH Phenyl Phenyl PH(EC)SDB-L ENV
LMSDSC-PS/DVB H2O-phobicDVB 101 HR-P
Normal PhaseSi-1(Silica) Silica DSC-Silica Silica SilicaGel Silica SiOHFL-PR(Florisil®) Florisil® Florisil® Florisil® Florisil® FL Florisil®
NH2 NH2 NH2 DSC-NH2 AminoPropyl Amino NH2 NH2
CN CN CN-U DSC-CN CN Cyano CN CNIon ExchangeABW ENVOLUTEABNSAX SAX DSC-SAX QuaternaryAmine QuaternaryAmine SAX SBSCX SCX DSC-SCX BenzeneSulfonic
AcidAromaticSulfonicAcid
SCX SA
WCX CBA DSC-WCX CarboxylicAcid CarboxylicAcid CBA PCAScreen-C CertifyI CleanScreen
DAUNarc-2 HCX Drug
Screen-CGF CertifyHF XtractDAUScreen-A CertifyII CleanScreenTHC Narc-1 HAX
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High Quality Economical Product Designed to Meet Your Sample Preparation Needs
Inert material leads to cleaner extracts Inthiscomparisontest,StrataC18-Egivescleanerextractsthanmanufacturer’salternativesolutions
Contact Phenomenex for details regarding this method.
Consistent manufacturing and QC ensures reproducible resultsRecoverydatafor3differentlotsofStrataC18-E200mg/3mL
Certificate of Analysis included with every box of Strata
NADOLOL
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METOPROLOL
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PROPRANOLOL
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Phenomenex Strata® C18-E
Waters® Sep-Pak® C18 IST ISoLUTE® C-18(EC) Varian® Bond Elut® C18
Improve your results now with a change to StrataCombiningover25yearsofindustryknowledgeandchromatographyexperience,PhenomenexhasdevelopedtheStrataSPElinewithinourextensiveSamplePreparationproductrange.StrataSPEencompassesavarietyofhighqualitysorbentsandformatsdesignedtoeffectivelycleanupandconcentratesamplesfrommatricesencounteredinallindustries.
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Determining Proper Bed MassSorbent Bed Mass = Analyte Capacity (i.e.30mg,50mg,60mg,100mg,150mg,200mg,500mg,1g,2g,5g,10g,20g,50g,70g)Thesorbentbedmassdeterminesthemaximumamountofanalytethatcanberetainedbythesorbent.
Silica-based sorbents Non-ionicsorbentsretainamassofsolute(analyteplusretainedcontaminants)thatisequivalenttoapproximately5%ofthesorbentmass.Therefore,a100mgcartridgecanretainapproximately5mgoftotalsolutemass.
Ion-exchange sorbents Capacityisdeterminedbythenumberofchargedsitesonthesorbentavailabletointeractwiththeanalyte.
Suggested Loading CapacitySilica-Based Sorbents (StrataC18-E,C8,SCX,SAX,WCX,NH2,etc.)
Selecting the Right Strata SPE Product• Sorbentbedmass
• Reservoirvolume
• Wash/elutionvolume
Sample Matrix Sorbent MassBlood,serum,plasma 50mgsorbentper250µLUrine 50mgsorbentper500µLFilteredtissuehomogenates 100mgsorbentper100mgtissue
Environmental Samples Sorbent MassWater(particulate-free)drinking 500mg/100-500mLsampleWater(particulate-laden)rivers,runoff,etc.
1g/100-500mLsample
SoilExtracts 1g/100mLofsoilextract
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Reduce breakthrough by selecting the proper bed mass!
Sorbent Wash & Elution Volumes*
ThevolumeofsolventneededforSPEprocessingisdirectlyrelatedtothemassofsorbentintheSPEtube,andmorespecifically,the“bedvolume”oftheSPEdevice.Intuitivelyweknowmoresorbentrequiresmoresolvent,lesssorbent=lesssolvent.Typically,4–16bedvolumesareusedinSPEmethods.
Reservoir = sample volume capacity(i.e.1mL,3mL,6mL,12mL,20mL,60mL,150mL)ThecolumnvolumereferstothedimensionsoftheSPEhousing(i.e.tube).Itdeterminesthelargestvolumesolventorsamplethatcanbedeliveredperaliquot.
Silica- BasedSorbent Mass
Practical Minimum Wash and Elution Volume 4 bed volumes
Recommended Wash and Elution Volume 8 bed volumes
Polymer- Based Sorbent Mass*
Practical Minimum Wash and Elution Volume 4 bed volumes
Recommended Wash and Elution Volume 8 bed volumes
10mg 60µL 120µL 10mg 100µL 200µL— — — 30mg 300µL 600µL50mg 300µL 600µL — — —— — — 60mg 600µL 1.2mL100mg 600µL 1.2mL 100mg 1mL 2mL150mg 900µL 1.8mL 150mg 1.5mL 3mL200mg 1.2mL 2.4mL 200mg 2mL 4mL500mg 3mL 6mL 500mg 5mL 10mL1g 6mL 12mL 1g 10mL 20mL2g 12mL 24mL — — —5g 30mL 60mL — — —10g 60mL 120mL — — —
*StratapolymericresinshavealargersurfaceareathanStratasilica-basedmaterialhencerequiringslightlymoresolventpergramforprocessing.Theelutionvolumesarespecifictothechemicalnatureoftheanalytebeingextracted,itsconcentrationinthesample,thechemicalnatureoftheelutingsolventandthebedmassused.Theaboveisaguideline,anelutionstudyshouldbeconductedtodeterminetheappropriatevolumetouse.
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Selecting the Proper Strata Phase
Strata Selection Tree
Reversed Phase Benefits Typical ApplicationC18-E Extractionofhydrophobicmoleculesfromaqueousandbiologicalsamples.
Extraction of hydrophobic or polar organic analytes from
aqueous matrix
C18-U Increasedextractionefficiencyandenhancedclean-upofhydrophobiccompoundsthatcontainhydroxyoraminefunctionalgroupsfromwaterorbiologicalfluids.
C18-T Widepore.Extractionoflargehydrophobicmolecules(upto75kD)fromwaterorbiologicalmatrices.
C8 ExtractionofhydrophobiccompoundsfromwaterorbiologicalfluidsthatareretainedtoostronglyonStrataC18-EorStrata-X.
Phenyl Extractionofaromatichydrophobiccompounds.CN Extractionofnon-polar,compoundsthatareretainedtoostronglyonStrataC18-EorC8.SDB-L Styrene-divinylbenzene.Extractionofnon-polarandpolarmolecules.Normal Phase Benefits Typical ApplicationCN Normalphasesorbentthatcaneffectivelyextractpolarcompoundsfromnon-polarsolvents.
Extraction of polar analytes from non-polar organic
solvents
NH2 Extractionofstronganionsfromaqueoussamples.EPH Fractionationofaliphaticandaromaticextractablehydrocarbonsfromenvironmentalsamples.Silica Extractionofpolarcompoundsthataresimilarinstructure.Florisil®(FL-PR) Extractionofpesticidesfromenvironmentalsamples.Alumina-N(AL-N) Extractionofpolarcompoundsfromfoodandenvironmentalsamples.Eco-Screen Extractionofnaphthalenefromenvironmentalsamples.Ion-Exchange Benefits Typical ApplicationWCX Weakcationexchange.Extractionofquaternaryamines.
Extraction of charged analytes from aqueous or non-polar
organic samples
SCX Strongcationexchange.Extractionof1°,2°and3°aminesfrombiologicalfluids.Screen-C Mixedmodecationexchange.Extractionofbasicdrugsfrombiologicalmatricessuchasblood,
serumandurine.WAX Weakanionexchange.Extractionofstrongand/orweakanionsfromaqueoussolvent.SAX Stronganionexchange.Extractionoforganicacids.Screen-A Mixedmodeanionexchange.Extractionofacidicdrugsfrombiologicalmatricessuchasblood,
serumandurine.ABW Specialtyphase.Fractionationofneutralcompoundssuchasamidesfromacidicandbasic
analytes.
aqueous
water
ionic
organic
organic
neutral
organic
neutral
nonpolar
reversedphase
SDB-L
C18-E
C8
PH
(+)basic
cationexchange
SCX
WCX
Screen-C
Screen-C GF
(-)acidic
anionexchange
SAX
NH2
Screen-A
polar
reversedphase
CN
neutral
moderatelypolar
reversedphase
C8
CN
C18-U
nonpolar
reversedphase
SDB-L
C18-E
C8
PH
moderatelypolar
normalphase
Si-1
NH2
EPH
Eco-Screen
AL-N
polar
normalphase
CN
Si-1
FL-PR
NH2
(+)basic
cationexchange
SCX
WCX
Screen-C
Screen-C GF
(-)acidic
anionexchange
SAX
NH2
Screen-A
ionic
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Quality Product Offerings
Strata C18-ESorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
96-Well Plate
On-Line Extraction Cartridge
Description Part No.Unit/Box Price
Strata C8Sorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
96-Well Plate
On-Line Extraction Cartridge
Description Part No.Unit/Box Price
Strata C18-USorbent Mass Part No. Unit/Box PriceTube
96-Well Plate
Strata PhenylSorbent Mass Part No. Unit/Box PriceTube
96-Well Plate
Strata SDB-LSorbent Mass Part No. Unit/Box PriceTube
96-Well Plate
Strata CNSorbent Mass Part No. Unit/Box PriceTube
Strata C18-T (widepore)Sorbent Mass Part No. Unit/Box PriceTube
Reversed Phase
For On-line Screening• 1-3minuteruntime
• Directinjectanalysis
For Traditional Sample Preparation
•Compatiblewithmostmanifoldsandroboticworkstations
•Consistentwell-to-wellandtube-to-tubeflow
If Strata SPE or Sepra bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, return the product with comparative data within 45 days for a FULL REFUND.
For Flash Analysis•Widerangeofpolarandnon-polar
selectivities
•Gramtokilogramquantities
For Flash and Large Scale-up
•Availablein12,20,60,and150mLvolumetubesizes
•Pre-packedformatseliminateexcesslaborassociatedwithglasspackedcolumns
Phenomenex|Web:www.phenomenex.com
50mg 8B-S001-DAK 1mL(100/box)100mg 8B-S001-EAK 1mL(100/box)100mg 8B-S001-EBJ 3mL(50/box)200mg 8B-S001-FBJ 3mL(50/box)500mg 8B-S001-HBJ 3mL(50/box)500mg 8B-S001-HCH 6mL(30/box)
500mg 8B-S001-HDG 12mL(20/box)2g 8B-S001-KDG 12mL(20/box)5g 8B-S001-LEG 20mL(20/box)10g 8B-S001-MFF 60mL(16/box)20g 8B-S001-VFF 60mL(16/box)50g 8B-S001-YSN 150mL(8/box)70g 8B-S001-ZSN 150mL(8/box)
25mg 8E-S001-CGB 2Plates/Box50mg 8E-S001-DGB 2Plates/Box100mg 8E-S001-EGB 2Plates/Box
StrataC18on-lineextractioncartridge,20x2.0mm
00M-S039-B0-CB
ea
Cartridgeholder,20mm
CH0-5845 ea
100mg 8B-S005-EAK 1mL(100/box)200mg 8B-S005-FBJ 3mL(50/box)500mg 8B-S005-HBJ 3mL(50/box)500mg 8B-S005-HCH 6mL(30/box)1g 8B-S005-JCH 6mL(30/box)
2g 8B-S005-KDG 12mL(20/box)5g 8B-S005-LEG 20mL(20/box)10g 8B-S005-MFF 60mL(16/box)
25mg 8E-S005-CGB 2Plates/Box50mg 8E-S005-DGB 2Plates/Box100mg 8E-S005-EGB 2Plates/Box
StrataC8on-lineextractioncartridge,20x2.0mm
00M-S101-B0-CB
ea
Cartridgeholder,20mm
CH0-5845 ea
100mg 8B-S002-EAK 1mL(100/box)200mg 8B-S002-FBJ 3mL(50/box)500mg 8B-S002-HCH 6mL(30/box)
50mg 8E-S002-DGB 2Plates/box
100mg 8B-S006-EAK 1mL(100/box)200mg 8B-S006-FBJ 3mL(50/box)500mg 8B-S006-HBJ 3mL(50/box)
50mg 8E-S006-DGB 2Plates/Box100mg 8E-S006-EGB 2Plates/Box
100mg 8B-S014-EAK 1mL(100/box)200mg 8B-S014-FBJ 3mL(50/box)500mg 8B-S014-HBJ 3mL(50/box)500mg 8B-S014-HCH 6mL(30/box)1g 8B-S014-JCH 6mL(30/box)
50mg 8E-S014-DGB 2Plates/Box
100mg 8B-S007-EAK 1mL(100/box)500mg 8B-S007-HBJ 3mL(50/box)500mg 8B-S007-HCH 6mL(30/box)
100mg 8B-S004-EAK 1mL(100/box)200mg 8B-S004-FBJ 3mL(50/box)500mg 8B-S004-HBJ 3mL(50/box)500mg 8B-S004-HCH 6mL(30/box)1g 8B-S004-JCH 6mL(30/box)
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Strata ABW (specialtyphase)Sorbent Mass Part No. Unit/Box PriceGiga™ Tube
Strata Silica (Si-1)Sorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
96-Well Plate
Strata SCX (strongcationexchange)Sorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
96-Well Plate
Strata CNSorbent Mass Part No. Unit/Box PriceTube
Strata Florisil®
(pesticideresiduegrade)Sorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
Strata Screen-A (mixed-modeanionexchange)Sorbent Mass Part No. Unit/Box PriceTube
96-Well Plate
Strata Screen-C (mixed-modecationexchange)Sorbent Mass Part No. Unit/Box PriceTube
Strata EPH (specialtyphase)Sorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
Strata NH2 / WAXSorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
96-Well Plate
Normal PhaseStrata NH2 / WAXSorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
96-Well Plate
Anion ExchangeStrata WCX (weakcationexchange)Sorbent Mass Part No. Unit/Box PriceTube
96-Well Plate
Cation Exchange
Strata SAX (stronganionexchange)Sorbent Mass Part No. Unit/Box PriceTube
Giga™ Tube
96-Well Plate
Trademarks StrataisaregisteredtrademarkofPhenomenex,Inc.Giga,Sepra,andStrata-XaretrademarksofPhenomenex,Inc.BondElutisaregisteredtrademarkofVarian,Inc.StyreScreenisaregisteredtrademarkofUCT.Sep-Pakisaregisteredtrade-markofWatersCorp.FlorisilisaregisteredtrademarkofU.S.SilicaCo.ISOLUTEisaregisteredtrademarkofBiotage.
Disclaimer Comparativeseparationsmaynotberepresentativeofallapplications.SubjecttoPhenomenexStandardTerms&Conditions,whichmaybeviewedatwww.phenomenex.com/TermsAndConditions.
©2008Phenomenex,Inc.Allrightsreserved.
Phenomenex|Web:www.phenomenex.com
2g 8B-S030-KDG 12mL(20/box)5g 8B-S030-LEG 20mL(20/box)
100mg 8B-S012-EAK 1mL(100/box)200mg 8B-S012-FBJ 3mL(50/box)500mg 8B-S012-HBJ 3mL(50/box)500mg 8B-S012-HCH 6mL(30/box)1g 8B-S012-JCH 6mL(30/box)
500mg 8B-S012-HDG 12mL(20/box)2g 8B-S012-KDG 12mL(20/box)5g 8B-S012-LEG 20mL(20/box)10g 8B-S012-MFF 60mL(16/box)20g 8B-S012-VFF 60mL(16/box)50g 8B-S012-YSN 150mL(8/box)70g 8B-S012-ZSN 150mL(8/box)96-Well Plate100mg 8E-S012-EGB 2Plates/Box
100mg 8B-S010-EAK 1mL(100/box)100mg 8B-S010-EBJ 3mL(50/box)200mg 8B-S010-FBJ 3mL(50/box)500mg 8B-S010-HBJ 3mL(50/box)500mg 8B-S010-HCH 6mL(30/box)1g 8B-S010-JCH 6mL(30/box)
2g 8B-S010-KDG 12mL(20/box)5g 8B-S010-LEG 20mL(20/box)10g 8B-S010-MFF 60mL(16/box)20g 8B-S010-VFF 60mL(16/box)
25mg 8E-S010-CGB 2Plates/Box100mg 8E-S010-EGB 2Plates/Box
100mg 8B-S007-EAK 1mL(100/box)500mg 8B-S007-HBJ 3mL(50/box)500mg 8B-S007-HCH 6mL(30/box)
500mg 8B-S013-HBJ 3mL(50/box)500mg 8B-S013-HCH 6mL(30/box)1g 8B-S013-JCB 6mL(30/box)
2g 8B-S013-KDG 12mL(20/box)5g 8B-S013-LEG 20mL(20/box)10g 8B-S013-MFF 60mL(16/box)
100mg 8B-S019-EAK 1mL(100/box)200mg 8B-S019-FBJ 3mL(50/box)500mg 8B-S019-HCH 6mL(30/box)
25mg 8E-S019-CGB 2Plates/Box
100mg 8B-S016-EAK 1mL(100/box)100mg 8B-S016-EBJ 3mL(50/box)150mg 8B-S016-SBJ 3mL(50/box)200mg 8B-S016-FBJ 3mL(50/box)300mg 8B-S016-RBJ 3mL(50/box)150mg 8B-S016-SCH 6mL(30/box)500mg 8B-S016-HCH 6mL(30/box)
500mg 8B-S031-HBJ 3mL(50/box)
5g 8B-S031-LEG 20mL(20/box)
100mg 8B-S009-EAK 1mL(100/box)200mg 8B-S009-FBJ 3mL(50/box)500mg 8B-S009-HBJ 3mL(50/box)500mg 8B-S009-HCH 6mL(30/box)1g 8B-S009-JCH 6mL(30/box)
500mg 8B-S009-HDG 12mL(20/box)2g 8B-S009-KDG 12mL(20/box)5g 8B-S009-LEG 20mL(20/box)
25mg 8E-S009-CGB 2Plates/Box50mg 8E-S009-DGB 2Plates/Box100mg 8E-S009-EGB 2Plates/Box
100mg 8B-S009-EAK 1mL(100/box)200mg 8B-S009-FBJ 3mL(50/box)500mg 8B-S009-HBJ 3mL(50/box)500mg 8B-S009-HCH 6mL(30/box)1g 8B-S009-JCH 6mL(30/box)
500mg 8B-S009-HDG 12mL(20/box)2g 8B-S009-KDG 12mL(20/box)5g 8B-S009-LEG 20mL(20/box)
25mg 8E-S009-CGB 2Plates/Box50mg 8E-S009-DGB 2Plates/Box100mg 8E-S009-EGB 2Plates/Box
100mg 8B-S027-EAK 1mL(100/box)200mg 8B-S027-FBJ 3mL(50/box)500mg 8B-S027-HBJ 3mL(50/box)500mg 8B-S027-HCH 6mL(30/box)1g 8B-S027-JCH 6mL(30/box)
25mg 8E-S027-CGB 2Plates/Box50mg 8E-S027-DGB 2Plates/Box100mg 8E-S027-EGB 2Plates/Box
100mg 8B-S008-EAK 1mL(100/box)100mg 8B-S008-EBJ 3mL(50/box)200mg 8B-S008-FBJ 3mL(50/box)500mg 8B-S008-HBJ 3mL(50/box)500mg 8B-S008-HCH 6mL(30/box)1g 8B-S008-JCH 6mL(30/box)
500mg 8B-S008-HDG 12mL(20/box)2g 8B-S008-KDG 12mL(20/box)5g 8B-S008-LEG 20mL(20/box)10g 8B-S008-MFF 60mL(16/box)20g 8B-S008-VFF 60mL(16/box)
25mg 8E-S008-CGB 2Plates/Box50mg 8E-S008-DGB 2Plates/Box100mg 8E-S008-EGB 2Plates/Box
6180
_I
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