7/29/2019 Stem Cells Pubmed

1/22

Stem/progenitor cells from inflamed human dental pulp retain

tissue regeneration potential

Dominick J Alongi1, Takayoshi Yamaza2,*, Yingjie Song3,Ashraf F Fouad1, Elaine ERomberg1, Songtao Shi2, Rocky S Tuan3,**, and George T-J Huang1,3,4,

1 University of Maryland, College of Dental Surgery, MD, USA

2 University of Southern California School of Dentistry, CA, USA

3 Cartilage Biology & Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal &Skin Diseases, NIH, MD, USA

4 Columbia University, College of Dental Medicine, NY, USA

Abstract

BackgroundPotent stem/progenitor cells have been isolated from normal human dental pulpstermed dental pulp stem cells (DPSCs). However, it is unknown whether these cells exist ininflamed pulps (IPs).

AimsTo determine whether DPSCs can be identified and isolated from IPs; and if they can besuccessfully cultured, whether they retain tissue regeneration potential in vivo.

Materials & methodsDPSCs from freshly collected normal pulps (NPs) and IPs werecharacterized in vitroand their tissue regeneration potential tested using anin vivostudy model.

ResultsThe immunohistochemical analysis showed that IPs expressed higher levels ofmesenchymal stem cell markers STRO-1, CD90, CD105 and CD146 compared with NPs (p