starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf ·...

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starBase v2.0 Tutorial © 2010-2013, Jianhua Yang at Sun Yat-sen University For comments, suggestions on the starBase platform, please contact us: [email protected] On commercial use, contact: [email protected] Cover Pan-Cancer Analysis

Transcript of starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf ·...

Page 1: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

starBase v2.0 Tutorial

© 2010-2013, Jianhua Yang at Sun Yat-sen University

For comments, suggestions on the starBase platform, please contact us: [email protected]

On commercial use, contact: [email protected]

Cover Pan-Cancer Analysis

Page 2: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Homepage

The distinctive features and improvements starBase v2.0

What starBase v2.0 includes

Link to starBase v1.0

Download the tutorial of starBase v2.0

TestimonialsHow to cite starBase

Examples

Page 3: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Pan-Cancer miRNA-lncRNA

Select Number of Cancer Types

Note:Number of Cancer Types(Pan-Cancer) can be used to explore anti-correlation (pearson correlation: r<0, p-value<0.05) between miRNA and target genes across diverse cancer types. For example, cancer type >=1 means that expression of miRNA and target gene is anti-correlation (pearson correlation: r<0, p-value<0.05) at least one cancer type.

Page 4: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Query Results: Pan-Cancer miRNA-lncRNA

Click miRNA to get detail and Pan-Cancer Expression Profiles

Click lncRNA to get detail and Pan-Cancer Expression Profiles

Click cancer Number to get detail and Pan-Cancer Expression Profiles for miRNA-lncRNA interactions

Page 5: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

① Pan-Cancer miRNA expression profiles

Query Results: Pan-Cancer miRNA-lncRNA

Select Pan-Cancer or single Cancer TypePrint or export figure

Expression Mean Value (log2) of normal samples

Fold-Change of Cancer/Normal samples

Mean Value (log2) of cancer samples

Cancer Types

Page 6: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

① Box-Plot miRNA expression profiles

Query Results: Pan-Cancer miRNA-lncRNA

Select single Cancer TypePrint or export figure

Normal samples

Cancer samples

sample information

Click Case Id to get clinical information

Fold-Change and t test p-value

Page 7: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

① Box-Plot lncRNA expression profiles

Query Results: Pan-Cancer miRNA-lncRNA

Select single Cancer TypePrint or export figure

Normal samples

Cancer samples

sample information

Click Case Id to get clinical information

Fold-Change and t test p-value

Page 8: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

① Pan-Cancer miRNA-lncRNA interactions

Query Results: Pan-Cancer miRNA-lncRNA

Cancer Types Sample Number r, p-value and False discovery rate (FDR)of pearson correlation analysis

click here

Page 9: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

① Pan-Cancer miRNA-lncRNA interactions

Query Results: Pan-Cancer miRNA-lncRNA

r value and p-value of pearson correlation analysis

click here

Select single Cancer TypeSelect Chart Type

Print or export figure

sample information

Click Case Id to get clinical information

Page 10: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-lncRNA

① miRNA-lncRNA interactions.

The target information page

Page 11: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-ncRNA

② miRNA-circRNA interactions.

The query page

The results and target information page are omitted because the interfaces are similar to the ones of miRNA-lncRNA interactions

Page 12: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-ncRNA

③ miRNA-pseudogene interactions.

The query page

The results and target information page are omitted because the interfaces are similar to the ones of miRNA-lncRNA interactions

Page 13: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-ncRNA

④ miRNA-sncRNA interactions.

The query page

The results and target information page are omitted because the interfaces are similar to the ones of miRNA-lncRNA interactions

Page 14: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-mRNA

① miRNA-target intersections.

The query page

The 4th option is restrained by the 5th one. If the count of programs selected in the 5th option is more than the program number chose in the 4th

option, the 4th option is disable.

Page 15: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-mRNA

Non-zero numbers are highlighted. Click on them for more details.

① miRNA-target intersections.

The results page

Export all query results as excel format

Page 16: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-mRNA

① miRNA-target intersections.

The target information page

Page 17: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-mRNA

Choose the interested clade, genome and assembly

Select a microRNA and a gene symbol

② miRNA-mRNA interactions.

The query page

Page 18: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-mRNA

② miRNA-mRNA interactions.

The results page

Non-zero numbers are highlighted. Click on them for more details.

Export all query results as excel format

Page 19: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

miRNA-mRNA

② miRNA-mRNA interactions.

The target information page

Display the location in the deepView genome browser

Link to experiments sources information

Link to NCBI nucleotide database

Page 20: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

ceRNA Network

The query page

The minimum number of shared miRNAs of typed gene and its predicted ceRNAs

Type the gene symbol for its ceRNAs prediction

Page 21: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

ceRNA Network

The results page

FDR values are highlighted. Click on them for more details.

Page 22: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

ceRNA Network

The ceRNA network information page

Link to NCBI nucleotide database

Page 23: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Protein-RNA

① protein-lncRNA interactions.

The query page

Select a RBP and a gene symbol(Optional)

Page 24: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Protein-RNA

① protein-lncRNA interactions.

The results page

The number of targetSites are highlighted. Click on them for more details.

Export all query results as excel format

Page 25: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Protein-RNA

① protein-lncRNA interactions.

The RNA-binding protein information page

Link to experiments sources information

Display the location in the deepView genome browser

Link to NCBI nucleotide database

Page 26: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Protein-RNA

② protein-mRNA interactions.

The query page

The results and target information page are omitted because the interfaces are similar to the ones of protein-lncRNA interactions

Page 27: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Protein-RNA

③ protein-pseudogene interactions.

The query page

The results and target information page are omitted because the interfaces are similar to the ones of protein-lncRNA interactions

Page 28: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Protein-RNA

④ protein-sncRNA interactions.

The query page

The results and target information page are omitted because the interfaces are similar to the ones of protein-lncRNA interactions

Page 29: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Functional Annotation

① miRFunction.

The query page

Multiple miRNAsselection are available

Page 30: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Functional Annotation

① miRFunction.

The results page

Up to 13 categories of function prediction using enrichment analysis

Running parameters set in the query page

The union of has-miR-33a-5p & has-miR-615-3p target genes

Every category of function prediction can be expanded/collapsed by the fold button in the table headers

Page 31: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Functional Annotation

② ceRNAFunction.

The query page

Gene symbol is required to enter

The same as the interface of ceRNA Network

The FDR threshold value of enrichment analysis

Page 32: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Functional Annotation

② ceRNAFunction.

The results page

Page 33: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Browser

Page 34: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Browser

miRNAtarget tracks

link to UCSC

tracks options

Page 35: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

Download

Link to PubMed Download BED files containing CLIP clusters data

Search the data listed in the table

starBase v2.0 download center.

Page 36: starBase v2.0 tutorialstarbase.sysu.edu.cn/starbase2/downloads/starBase_v2.0_tutorial.pdf · starBase Examples. Pan-Cancer miRNA-lncRNA Select Number of Cancer Types Note: Number

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