Standard Operating · PDF fileChemical Class Standard Operating Procedures Particularly...

Rev. Date: June 10, 2013 1 Particularly Hazardous Substances Class SOP

Chemical Class Standard Operating Procedures

Particularly Hazardous Substances (PHS)

Print a copy and insert into your laboratory SOP binder.

Department: Chemistry

Date SOP was written: June 10, 2013

Date SOP was approved by PI/lab supervisor:

Principal Investigator: Name: Richmond Sarpong

Signature: ______________________________

Internal Lab Safety Coordinator or Lab Manager:

Name: Rebecca Murphy

Lab Phone: 510-643-2485

Office Phone: 510-642-6312

Emergency Contact: Name: Richmond Sarpong

Phone Number: 626-644-2407

Location(s) covered by this SOP: Latimer Hall: 834, 836, 837, 838, 839, 842, 847, 849, 907

1. Purpose

This SOP covers the precautions and safe handling procedures for the use of Particularly Hazardous Substances in the Sarpong group, which include the following chemicals and their uses:

Chemical(s) Use(s)

PHSs 1. Using Particularly Hazardous Substances as reagents.

PHS solvents 2. Using Particularly Hazardous Substances as solvents in reactions, for extractions or cleaning glassware.

PHS solvents 3. Using Particularly Hazardous Substances as solvents in column chromatography (CC) or thin layer chromatography (TLC).

Sarpong inventory chemicals covered by this SOP

See below.


Sarpong Group inventory chemicals covered by this SOP

(R)-(+)-2,3-Dihydroindole-2-carboxylic acid

(r)-styrene oxide

(S)-(-)-Indoline-2-carboxylic acid

1,1,3,3-Tetramethylurea

1,2,4-Triazole

1,2-bis(diphenylphosphino)ethane nickel (ii) chloride

1,2-dichloroethane

1,2-Epoxy-5-hexene

1,2-phenylenediamine

1,3,5-trioxane

1,3-Bis(diphenylphosphino)propanenickel(II) chloride

1,3-diphenylguanidine

1,3-Phenylenediamine

1,4-cyclohexanediene

1-bromo-2-chloroethane

1-Methyl-2-pyrrolidinone

2,3-dichloro-1-propene

2,4-dinitrobenzenesulfonyl chloride

2-aminophenol

2-bromopropane

2-Methoxyethanol

2-methyl-5-nitroaniline

2-Nitropropane

2-nitrotoluene

4,4'-bis(dimethylamino)benzophenone

4,4'-dibromobiphenyl

4-chloroaniline

5-Bromoisatoic anhydride

5-methylfurfural

9-BBN triflate solution 0.5 M in hexanes

acrylamide

allyl chloride

aniline

aniline hydrochloride

Benzamide

Benzyl Chloromethyl Ether

bis(2,4,6-trimethylpyridine)iodine(I) hexafluorophosphate

bis(triphenylphosphine)nickel(ii) chloride

boric acid

Carbon Disulfide

chloramphenicol

Chloroform

cis-1,4-Dichloro-2-butene

Cobalt chloride hexahydrate

Cobalt(II) acetylacetonate

Cyclopropylboronic acid

Diethyl azodicarboxylate

Diethylene glycol dimethyl ether

epichlorohydrin

Ethoxyethyne

Ethylhydrazine oxalate

Formamide

Gallium(III) Iodide

Glufosinate Ammonium

Glyoxal

Haloperidol

hexamethylphosphoramide

Hexamethylphosphorous triamide

Hexane

Hydrazine hydrate

hydrazine monohydrate

Hydroquinone

imidazole

Indium (III) iodide

Isobutyl nitrite

isoprene

Lead (II) bromide

Lead (IV) acetate

Lithium acetylacetonate

Magnesium methyl carbonate

N,N-Dimethylacetamide

Naphthalene

Nickel (II) acetylacetonate

Nickel (II) sulfate hexahydrate

Nickel(II) chloride

nickel(II) iodide

Nickel(II)Chloride Hexahydrate

nitrobenzene

N-methylacetamide

o-Aminoanisole

o-Phenylenediamine

Phenol


Phenolphthalein

Phenylhydrazine hydrochloride

Phenylhydrazine

phloroglucinol

Propylene oxide

Pyridinium chlorochromate

Pyrogallol

quinoline

Sodium Perborate Monohydrate

sodium tetraborate

Styrene Oxide

Tebbe reagent

Thiourea

trimethylphosphine 1M in toluene

Tris(2-methoxyethoxy)(vinyl)silane

Vinyl bromide

vinyl bromide, 1.0M in THF

Vitride(R) T reducing agent

chromium (VI) oxide

1,1-Dimethylhydrazine

2,4-Dinitroaniline

2-chloroacrylonitrile

4-aminophenol

benzenethiol

Benzyl chloride

benzyl chloroformate

Boron tribromide

boron tribromide 1 M in DCM

cis-dichlorodiamine platinum(II)

Cycloheximide

cyclohexylamine

Dimethylcarbamyl chloride

dimethyl sulfate

N,N-Dimethylsulfamoylchloride

Di-n-butyltin dichloride

Glutaraldehyde

hexafluoroacetone

Hexafluoroacetone trihydrate

Hydrazine

isopropyl chloroformate (1M in toluene)

mercury bichloride

o-Tolidine dihydrochloride

Sodium dichromate dihydrate (VI)

Potassium dichromate

(Trimethylsilyl)diazomethane, 2M in diethyl ether

1,1,2,2-Tetrachloroethane

1,1,3,3-Propanetetracarbonitrile

1,2,4-trifluoro-5-nitrobenzene

1,2-Ethanedithiol

1,3-diaminopropane

1,3-Dichloroacetone

1,3-Dicyclohexylcarbodiimide

1,3-diisopropylcarbodiimide

1,4-naphthoquinone

1,5-Difluoro-2,4-dinitrobenzene

12-Crown-4

1-cyanovinyl acetate

2,4,6-Trimethylaniline

2,4-dichlorophenol

2,6-dibromopyridine

2-acetylfuran

2-Acetylthiophene

2-amino-6-picoline

2-Bromo-5-(trifluoromethyl)pyridine

2-bromopyridine

2-chlorobenzyl chloride

2-chloroethanol

2-chloropyridine

2-Cyclohexen-1-one

2-Cyclohexenone

2-ethylacrolein

2-Ethylpropenal

2-furaldehyde

2-mercaptoethanol

2-Pyridinecarboxaldehyde

3,4-diaminopyridine

3-butyn-2-ol

3-Butyn-2-one

3-chlorophenyl isocyanate

3-chloropropionyl chloride

4-(Dimethylamino)pyridine

4-Chlorobutanyryl chloride

4-Methoxy-2-nitroaniline

5-chloro-2-nitroaniline


Acetic Acid Triethylamine 2 M 2 M

acetone cyanohydrin

Acetylferrocene

Aconitine

adiponitrile

allyl acetate

allyl alcohol

allyl chloroformate

allyl iodide

allylamine

benzeneseleninic anhydride

Benzoyl cyanide

benzyl mercaptan

Benzyltriphenylphosphonium chloride

Bis(4-nitrophenyl) hydrogenphosphate

Boron Trifluoride Ether Complex

bromoacetaldehyde diethyl acetal

Bromoacetic Acid

Butyl isocyanate

Cesium azide

Cetylpyridinium chloride

chloroacetaldehyde

chloroacetone

chloroacetonitrile

Cumene Hydroperoxide

cyanuric chloride

cyclohexyl isocyanate

Cyclopentanamine

Decaborane

Dibutyl tin dilaurate

Dibutyltin(IV) Oxide

dichlorodiphenylsilane

diethyl chlorophosphate

Dimethylchloro phosphate

Dimethyldicarbonate

Di-t-butyldicarbonate

Eaton's reagent

ethyl bromoacetate

Ethyl chloroformate

ethyl iodoacetate

Ethylphosphonic dichloride

Hexamethylditin

hydrazine sulfate

Hydrogen fluoride pyridine

Iodomethane

iron(0) pentacarbonyl

isobutyronitrile

isopropyl isocyanate

Malononitrile

mercuric cyanide

Mercuric oxide yellow

Mercury (II) oxide

mercury (II) sulfate

Mercury iodide

mercury(ii) acetate

mercury(ii) trifluoroacetate

methanesulfonyl chloride

Methyl cyanoformate

methyl vinyl ketone

methylphosphonic dichloride

molybdenum carbonyl

m-xylylenediamine

n,n'-dicyclohexylcarbodiimide

N,N'-diisopropylcarbodiimide

N,N-dimethyl-1,3-propanediamine

N,N-Dimethylphenylenediamine

o-cresol

Osmium Tetroxide

Osmium tetroxide Solution

p-Anisidine

p-Cresol

p-Dinitrobenzene

phenyl chloroformate

phenyl isocyanate

Phosphorus oxychloride

Phosphorus pentachloride

phosphorus pentoxide

phosphorus trichloride (2.0 m in dcm)

Phthalonitrile

Pivaloyl chloride

Propargyl Alcohol

Propionitrile

pyridine-2-carboxaldehyde

Selenium


sodium borate

Sodium cyanoborohydride

sodium tetraborate decahydrate

Sodium Trifluoroacetate

Strychnine

sulfuryl chloride

Tetrabutylammonium cyanide

tetramethyltin

Tetranitromethane

thallium(I) carbonate

Thallium(I) ethoxide

Thallium(III) acetate

thallium(III) nitrate trihydrate

thioglycolic acid

Titanium tetrachloride

trans-crotonaldehyde

Tributylamine

Trichloroacetyl chloride

Triethoxysilane

Trimethyl acetyl chloride

Trimethyl(phenyl)tin

Trimethylsilyl cyanide

Trimethylsilyldiazomethane

Trimethylsilyldiazomethane (2.0 M in hexanes)

Trimethylsilyldiazomethane, 2.0 M solution in hexanes

Trimethyltin azide

Trimethyltin chloride

Triphosgene

1,2-dibromoethane

Chloroform-D

Pyridinium dichromate

N-Nitroso-N-Methylurea

4-Toluenesulfonyl azide

Sodium perborate tetrahydrate

tert-butyl hydroperoxide

1,3-Dinitrobenzene

peracetic acid 32 wt % in dilute acetic acid

4-dimethylaminopyridine

Nickel peroxide


2. Physical & Chemical Properties/Definition of Chemical Group

Particularly Hazardous Substances fall into three major categories: reproductive toxins, acute toxins, and select carcinogens.

Reproductive Toxins are chemicals that affect the reproductive capabilities including causing chromosomal damage (mutations) and adverse effects on fetal development (teratogenesis).

Reproductive toxins are designated by one or more of the following H codes:

H340 May cause genetic defects

H341 Suspected of causing genetic defects

H360 May damage fertility or the unborn child

H361 Suspected of damaging fertility or the unborn child

H362 May cause harm to breast-fed children

Acute Toxins are chemicals that pose a high level of immediate health risk to individuals. Acutely Toxic Chemicals are those listed specifically in the Settlement Agreement and/or those chemical compounds with a high level of acute toxicity as defined by the Globally Harmonized System of Classification and Labeling of Chemicals (GHS) Categories 1 and 2:

Acute Toxicity Category 1 Category 2

Oral (mg/kg) < 5 Between > 5 and ≤ 50

Dermal (mg/kg) < 50 Between > 50 and ≤ 200

Gases (ppm) < 100 Between > 100 and ≤ 500

Vapors (mg/l) < 0.5 Between > 0.5 and ≤ 2.0

Dusts and Mists (mg/l) < 0.05 Between > 0.05 and ≤ 0.5

Acutely toxic chemicals may also have other hazardous properties in addition to acute toxicity. Safe use requires assessing all potential hazards.

Acutely toxic chemicals are designated by one or more of the following H codes:

H300 Fatal if swallowed

H310 Fatal in contact with skin

H330 Fatal if inhaled

Select Carcinogens are a category of chemicals where the available evidence strongly indicates that the substances cause human carcinogenicity. A “select carcinogen” meets one of the following criteria.

1. It is regulated by Cal/OSHA as a carcinogen. 2. It is listed under the category “known to be carcinogens” in the annual report by the National

Toxicology Program (NTP). 3. It is listed under Group 1 – “carcinogenic to humans” – by the International Agency for Research on

Cancer (IARC) 4. It is listed in either Group 2A or Group 2B by the IARC or under the category “reasonably anticipated

to be carcinogens” by the NTP, and causes statistically significant tumor incidence in experimental animals in accordance with any of the following criteria:


a. After inhalation exposure of 6-7 hours per day, 5 days per week, for a significant portion of a lifetime to dosages of less than 10 mg/m3

b. After repeated skin application of less than 300 mg/kg of body weight per week; or c. After oral dosages of less than 50 mg/kg of body weight per day.

Select carcinogens are designated by one of the following H codes:

H350 May cause cancer

H351 Suspected of causing cancer

Cal/OSHA Regulated Carcinogens fall into a separate hazard class and have extensive additional requirements associated with them that are not covered under this procedure. The use of these agents may require personal exposure sampling based on usage. The specific Cal/OSHA regulations that govern the use of regulated carcinogens can be found at

http://www.dir.ca.gov/Title8/sb7g16a110.html

http://www.dir.ca.gov/Title8/5209.html

Some exemptions from these requirements are made for laboratory scale use; these can be found at:


Contact the EH&S department if there are any questions about the use of these materials.

3. Potential Hazards/Toxicity

Careful handling and stringent controls of these chemicals are essential in order to protect workers and the environment. Requirements include designating a specific location for working with these substances including labeling and warning signs for those who may enter or work in the designated areas.

Additional requirements may apply, depending on the specific chemical. Examples include particularly hazardous substances that are also highly flammable and/or reactive.

4. Engineering Controls

Use the engineering controls listed below unless other lab-specific information is included in the Protocol/Procedure section.

• A laboratory type fume hood with the sash position closed as much as possible;

• A glove box for pyrophorics and water reactive chemicals. Glove boxes may also be required for other chemicals, such as regulated carcinogens and particularly hazardous substances;

• Supplemental protective equipment like a blast shield, where appropriate, to protect from explosions when using peroxide formers, pyrophorics, water reactives, and potentially explosive chemicals.

• Laboratories and rooms where particularly hazardous substances are used shall have general room ventilation that is at negative pressure with respect to the corridors and external environment. The laboratory/room door must be kept closed at all times.

5. Personal Protective Equipment

For additional information on PPE requirements, go to: http://ccehss.berkeley.edu/section5#Personnel_Protective_Equipment_Required_in_College_Laboratories




http://ccehss.berkeley.edu/section5%23Personnel_Protective_Equipment_Required_in_College_Laboratories


Use the PPE listed below unless other lab-specific information is included in the Protocol/Procedure section.

Eye and Face Protection

ANSI-approved safety glasses with side shields or chemical splash goggles must be worn at all times when handling chemicals in the lab.

Skin and Body Protection

1. Gloves are required when handling hazardous chemicals. a. Specific glove type recommendations are provided in the Protocol/Procedure section. b. Inspect gloves prior to use. Use proper glove removal technique (without touching

glove's outer surface) to avoid skin contact with this product. Wash and dry hands after handling chemicals, before breaks, and at the end of the workday.

c. For additional information on glove selection, go to: http://ehs.berkeley.edu/hs/63-laboratory-safety/94-glove-selection-and-usage.html

2. Lab coats are required when handling hazardous chemicals in the lab. a. Nomex 3A flame-resistant lab coats are required when working with pyrophorics (H250)

and explosives (H200, H201, H202, H203) b. Flame resistant lab coats (Nomex or other material) should be worn when working with

hazardous chemicals such as Category 1 or 2 flammable liquids (H224 and H225). 3. Cotton-based, non-synthetic clothing (including long pants; no skin exposed below the waist)

should be worn. 4. Closed-toe and closed-heel shoes are required in the lab.

Respiratory Protection

Respiratory protection is normally not required for UC Berkeley laboratory activities. Any lab personnel considering the use of a respirator must contact EH&S for a workplace assessment. Respirator users will be provided with specific instructions if a respirator is deemed necessary by EH&S.

6. First Aid Procedures and Medical Emergencies

Notify supervisor and EH&S immediately. Follow up with a call to 510-642-9090 to report the incident.

Life Threatening Emergency, After Hours, Weekends and Holidays – Call 911 or go to the nearest emergency room. Note: All serious injuries must be reported to EH&S within 8 hours. Follow up with a call to 510-642-9090 to report the incident.

Assess the extent of danger. If you cannot assess the conditions of the environment well enough to be sure of your own safety, do not enter the area. If possible, help contaminated or injured persons. Obtain medical attention for the individual as soon as possible by calling 911. Provide a copy of the appropriate SDS to the emergency responders or physician, as needed.

If inhaled

Move into fresh air. Go to the Occupational Health Facility (Tang Health Center) and after hours, go to the nearest emergency room. If person is not breathing, call 911 and give artificial respiration. If unconscious, call 911.

In case of skin contact

Immediately flush with flowing water for no less than 15 minutes; remove any jewelry or clothing as necessary to facilitate clearing of any residual materials. Wash off with soap and plenty of water for 15

http://ehs.berkeley.edu/hs/63-laboratory-safety/94-glove-selection-and-usage.html


minutes. If skin contact requires medical assistance, go to the Occupational Health Facility (Tang Health Center) and after hours, go to the nearest emergency room. If this is a large or serious injury, call 911.

In case of eye contact

Rinse thoroughly with plenty of water using an eyewash station for at least 15 minutes, occasionally lifting the upper and lower eyelids. Remove contact lenses if possible. Call 911.

If swallowed

Call 911. Do not induce vomiting unless directed otherwise by the SDS. Never give anything by mouth to an unconscious person. Rinse mouth with water. Go to the Occupational Health Facility (Tang Health Center) and after hours, go to the nearest emergency room.

Needle stick/puncture exposure

Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure such as eyes, mouth and/or nose, flush the affected area for 15 minutes using an eyewash station. Go to the Occupational Health Facility (Tang Health Center) and after hours, go to the nearest emergency room.

All needle stick/puncture exposures must be reported to EH&S within 8 hours. Follow up with a call to 510-642-9090 to report the incident.

7. Special Handling and Storage Requirements

Lab-specific information on handling and storage may be included in the Protocol/Procedure section.

Working alone – Certain extremely hazardous operations should not be performed if the PI or Lab Safety Contact(s) are not present. Never work alone with extremely hazardous materials/operations. See the Protocol/Procedure section below for specific prohibitions (if any) on working alone.

Areas in the laboratory where PHS are used must be identified as a “Designated Area” for use. PHS may only be used and stored in the designated areas. Additionally, be sure to label this area using appropriate signage – e.g., CAUTION, DESIGNATED AREA – REPRODUCTIVE TOXINS, ACUTE TOXINS, OR SELECT CARCINOGENS MAY BE PRESENT.

• Label waste containers containing PHS with warning labels.

• Label storage space with warning labels.

• Store non-flammable PHS within secondary containment.

• Store flammable PHS within flammable storage cabinet and designate a bottom shelf or secondary container.

• Provide secondary containment for chemicals in accordance with the ccEHS "Chemical Hygiene Plan": http://ccehss.berkeley.edu/section5#Chemical_Handling_Storage_and_Transportation

8. Chemical Spill

Spill – Assess the extent of danger; if necessary request help by calling 911 and 510-642-9090. If you cannot assess the conditions of the environment well enough to be sure of your own safety, do not enter the area. If possible help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors from spill. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

http://ccehss.berkeley.edu/section5#Chemical_Handling_Storage_and_Transportation


Minor Spill – In the event of a minor spill, if there is no potential for hazardous chemical exposure, report the spill to 510-642-9090 and proceed to clean it, if you are trained. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Call 510-642-9090 to report the spill to ccEHSS and for assistance.

Major Spill – Any hazardous chemical spill that involves chemical exposure, any chemical spill that due to size and/or hazard requires capabilities beyond your training, or any chemical spill that gives the perception (because of odor, for example) that there has been a hazardous release.

Call 911 and 510-642-9090 to report the spill to ccEHSS and for assistance.

9. Cleaning and Decontamination

Lab-specific information on decontamination may be included in the Protocol/Procedure section.

• Wearing proper PPE, laboratory work surfaces shall be cleaned at the conclusion of each

procedure and at the end of each work day.

• Dispose of contaminated materials in accordance with hazardous waste disposal guidelines

referenced below.

• Decontaminate all equipment before removing from a designated area.

10. Hazardous Waste Disposal

Label Waste

Label all containers with the label provided at: http://ehs.berkeley.edu/hm/279-new-hazardous-waste-program-hwp.html. See the EH&S Fact Sheet, “Hazardous Waste Management” for general instructions on procedures for disposing of hazardous waste.

Dispose of Waste

• Dispose of regularly generated chemical waste within 6 months. • Call EH&S with questions.

11. Safety Data Sheet (SDS) Location

SDS can be accessed online at http://ucmsds.com

http://ehs.berkeley.edu/hm/279-new-hazardous-waste-program-hwp.html

http://ucmsds.com/


12. Protocol/Procedure – Particularly Hazardous Substances

Preparation Know the location of the nearest fire extinguisher, eyewash, and safety shower before beginning work.

These materials are classified as Particularly Hazardous Substances (PHS), and contact must be minimized at all times. Because of the risks associated with longer term repeated exposure. The hazards could include being suspected carcinogens, reproductive toxins, etc. Therefore, in addition to immediate exposures, extra care must be taken to prevent contamination of clothing, work surfaces, etc. when working with these materials. Many of these materials are flammable or combustible and so avoid open flames and other ignition sources. Use in the smallest practical quantities for the experiment being performed.

Chemical Storage and Disposal

Storage:

Store away from incompatible chemicals.

Keep in a tightly closed container, stored in a cool, dry, ventilated area.

Store away from heat and sources of ignition.

Disposal:

All contaminated wastes shall be disposed of into waste containers specifically designated for hazardous chemical waste.

Mix ONLY with compatible waste streams. Store hazardous waste containers in secondary containment. Liquid waste will

be labeled appropriately and disposed of through EH&S once liquid waste container is full.

Decontamination of Equipment

Equipment that needs to be relocated (for repair or change of location etc.) must be properly decontaminated.

Lab-specific Information

Add lab-specific information not included above if needed (e.g., all work for this procedure is to take place in the designated fume hood.)


Procedure/Use Scale Engineering Controls/Equipment

PPE (eye, face, gloves, clothing) Procedure Steps and Precautions

1. Using Particularly Hazardous Substances as reagents

2 g or less in 50 to 100 mL of solvent

All reactions using these materials must be performed in a properly operating fume hood. Blast shields must be used if the reaction has the potential to generate large quantities of gases or is strongly exothermic.

Eye protection: Wear ANSI Approved tight-fitting safety goggles or safety glasses with side shields.

Face Protection: The need for face protection is based on the solvents used or if the reaction will be externally heated or if needed. Hand Protection : Confirm compatibility of glove material with material being used. General guidance: (low contact): 8 mil minimum Nitrile gloves must be used to prevent incidental contact. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Remove gloves immediately upon contamination. Wash and dry hands after use. Hand Protection (high contact): Extended contact with many solvents may penetrate lab gloves Use Laminate Film gloves (e.g. Ansell Barrier) for extended contact (such as spill cleanup) and for any potential contact with solutions containing highly toxic solutes. Extended contact with benzene should be avoided. Remove gloves immediately upon contamination.

Avoid the formation of dusts with solids. Carefully weigh materials as normal. Use of enclosed balance or tared method with secondary containment is recommended where feasible. Immediately move to fume hood when weighing is complete. In a properly functioning fume hood, add reagent to the reaction vessel. If the reagent is a liquid, dispense and transfer to the reaction vessel using either a syringe or pipettor.


Clothing: Wear fire/flame resistant lab coat (100% cotton based); cotton based clothing/attire; full length pants or equivalent; and close-toed, close-heeled shoes.

Notes Any deviation from this SOP requires approval from PI.




2. Using Particularly Hazardous Substances as solvents in reactions, for extractions or cleaning glassware

up to 100 mL when used as a reaction solvent no more than 500 mL when using to clean glass-ware no more than 500 mL when using for extrac-tions

All operations using these materials must be performed in a properly operating fume hood. Blast shields must be used if the reaction has the potential to generate large quantities of gases or is strongly exothermic. Open flames and possible sparking and static electricity must be avoided.

Eye protection: Wear ANSI Approved tight-fitting safety goggles or safety glasses with side shields.

Face Protection: The need for face protection is based on the solvents used or if the reaction will be externally heated or if needed. Hand Protection : Confirm compatibility of glove material with material being used. General guidance: (low contact): 8 mil minimum Nitrile gloves must be used to prevent incidental contact. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Remove gloves immediately upon contamination. Wash and dry hands after use. Hand Protection (high contact): Extended contact with many solvents may penetrate lab gloves Use Laminate Film gloves (e.g. Ansell Barrier) for extended contact (such as spill cleanup) and for any potential contact with solutions containing highly toxic solutes. Extended contact with benzene should be avoided. Remove gloves immediately upon contamination.

Avoid inhalation of these materials. If a rotary evaporator is used ensure it is vented in the fume hood. If it is necessary to condense the solvent, use an open cold trap system. Pressure can be built up when these solvents are used in closed reaction vessels. Adequate ventilation (e.g. pressure bubbler on Schlenk manifold, or an equilibrating balloon) must be used to prevent dangerous over pressurization. Pressure may be built up if when performing extractions. Adequate ventilation (open the valve frequently during the extraction) has to be used to prevent dangerous over pressurization. The vapor in such a process must be released in a fume hood. If heated, the reaction apparatus has to be fitted with an adequately sized condenser and an adequate



flow of cooling water has to be provided to prevent evaporation. Cooling hoses have to be secured with hose clamps to the condenser and the outlet. When used to clean glassware, leave the cleaned glassware in the fume hood until the solvent has evaporated off.





3. Using Particularly Hazardous Substances as solvents in column chromatography (CC) or thin layer chromatography (TLC)

up to 50 mL when used in TLC no more than 2000 mL when using for CC

All chromatography operations using these materials must be performed in a properly operating fume hood. Open flames and possible sparking and static electricity must be avoided.

Eye protection: Wear ANSI approved tight-fitting safety goggles or safety glasses with side shields. Face Protection: Wear a face shield when handling containers that are not behind a lab hood sash or blast shield. Hand Protection : Confirm compatibility of glove material with material being used. General guidance: (low contact): 8 mil minimum Nitrile gloves must be used to prevent incidental contact. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Remove gloves immediately upon contamination. Wash and dry hands after use. Hand Protection (high contact): Extended contact with many solvents may penetrate lab gloves Use Laminate Film gloves (e.g. Ansell Barrier) for extended contact (such as spill cleanup) and for any potential contact with solutions containing highly toxic solutes. Extended contact with benzene should be avoided. Remove gloves immediately upon contamination.

Avoid inhalation of these materials. Thin Layer Chromatography: allow the plate to dry in the fume hood after removal from the development bath. Column Chromatography: collect fractions of interest and concentrate as needed. If a rotary evaporator is used for concentrating, ensure it is vented in the fume hood. If it is necessary to condense the solvent, use an open cold trap system. After use in the column, allow the solvent to evaporate from the packing material prior to proper disposal of the packing material.


Sarpong Group Notes on Particularly Hazardous Substances

Physical & Chemical Properties/Definition of Chemical Group

Particularly Hazardous Substances fall into three major categories: reproductive toxins, acute toxins, and select carcinogens.

Reproductive Toxins are chemicals that affect the reproductive capabilities including causing chromosomal damage (mutations) and adverse effects on fetal development (teratogenesis).

For a list of reproductive toxins, please consult the current List of Carcinogens and Reproductive Toxins per Proposition 65 http://oehha.ca.gov/prop65/prop65_list/Newlist.html.

Acute Toxins are chemicals that pose a high level of immediate health risk to individuals. They can be defined as:

1. A chemical with a median lethal dose (LD50) of 50 mg or less per kg of body weight when administered orally to albino rats weighing between 200 and 300 g each.

2. A chemical with a median lethal dose (LD50) of 200 mg or less per kg of body weight when administered by continuous contact for 24 hours (or less if death occurs within 24 hours) with the bare skin of albino rabbits weighing between 2-3 kg each.

3. A chemical that has a median lethal concentration (LC50) in air of 5000 ppm by volume or less of gas or vapor, or 50 mg per liter or less of mist, fume, or dust, when administered by continuous inhalation for 1 hour (or less if death occurs within 1 hour) to albino rats weighing between 200 and 300 g each.

Select Carcinogens are a category of chemicals where the available evidence strongly indicates that the substances cause human carcinogenicity. A “select carcinogen” meets one of the following criteria.

1. It is regulated by Cal/OSHA as a carcinogen. 2. It is listed under the category “known to be carcinogens” in the annual report by the National

Toxicology Program (NTP). 3. It is listed under Group 1 – “carcinogenic to humans” – by the International Agency for Research

on Cancer (IARC) 4. It is listed in either Group 2A or Group 2B by the IARC or under the category “reasonably

anticipated to be carcinogens” by the NTP, and causes statistically significant tumor incidence in experimental animals in accordance with any of the following criteria:

a. After inhalation exposure of 6-7 hours per day, 5 days per week, for a significant portion of a lifetime to dosages of less than 10 mg/m3

b. After repeated skin application of less than 300 mg/kg of body weight per week; or

http://oehha.ca.gov/prop65/prop65_list/Newlist.html


c. After oral dosages of less than 50 mg/kg of body weight per day.

Cal/OSHA Regulated Carcinogens fall into a separate hazard class and have extensive additional requirements associated with them that are not covered under this procedure. The use of these agents may require personal exposure sampling based on usage. The specific Cal/OSHA regulations that govern the use of regulated carcinogens can be found at



Some exemptions from these requirements are made for laboratory scale use; these can be found at: http://www.dir.ca.gov/Title8/5191.html

Contact the EH&S department if there are any questions about the use of these materials.

Engineering Controls

• Work with particularly hazardous substances should be avoided and is not permitted if there is a reasonable likelihood of workers exceeding regulatory exposure limits.

• Chemical fume hoods used as containment areas for particularly hazardous substances must have a face velocity of 100 lfm, averaged over the face of the hood and must be certified annually.

• Laboratories and rooms where particularly hazardous substances are used shall have general room

ventilation that is at negative pressure with respect to the corridors and external environment. The laboratory/room door must be kept closed at all times.

Special Handling and Storage Requirements

Storage

Use unbreakable secondary containment for the storage of acutely toxic chemicals. If the materials are volatile (or could react with moisture or air to form volatile toxic compounds), containers should be in a ventilated storage area. All containers of acutely toxic chemicals should be clearly labeled with chemical composition, known hazards, and warnings for handling.

Chemicals that can combine to make toxic materials (e.g., acids and inorganic cyanides, which can generate hydrogen cyanide) should not be stored in the same secondary containment. Chemicals that have a limited shelf life need to be tracked and monitored. Chemicals that require refrigeration should be stored appropriately.

Working Alone

Certain extremely hazardous operations should not be performed if the PI or Lab Safety Contact(s) are not present. Never work alone with extremely hazardous materials/operations. See the Protocol/Procedure section below for specific prohibitions (if any) on working alone.





Areas in the laboratory where PHS are used must be identified as a “Designated Area” for use. PHS may only be used and stored in the designated areas. Additionally, be sure to label this area using appropriate signage – e.g., CAUTION, DESIGNATED AREA – REPRODUCTIVE TOXINS, ACUTE TOXINS, OR SELECT CARCINOGENS MAY BE PRESENT.

• Label waste containers containing PHS with warning labels.

• Label storage space with warning labels.

• Store non-flammable PHS within secondary containment.

• Store flammable PHS within flammable storage cabinet and designate a bottom shelf or secondary container.


13. Documentation of Training (signature of all users is required)

• Prior to conducting any work with Particularly Hazardous Substances, designated personnel must

provide training to his/her laboratory personnel specific to the hazards involved in working with this

substance, work area decontamination, and emergency procedures.

• The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a

copy of the Particularly Hazardous Substances SDSs provided by the manufacturer.

I have read and understand the content of this SOP:

Name Signature Identifier Date

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