Stagetop Environmental Control
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WORLD PRECISION INSTRUMENTS 08/09/2019 For short term or long term studies of living cell cultures under a microscope or for time lapse video research, a microscope stagetop incubator is essential. Perfect for Live Cell Imaging, STEV (the stagetop environmental control system) is a heated, humidified, compact environmental case that houses your culture wells and fits on a microscope stage. The STEV can be used with 35mm well plates by simply replacing the seal on the bottom of the stagetop environment case. This system offers precision control of temperature, CO 2 and O 2 . The system is flexible and easy to configure for a variety of experimental conditions. The system includes the Environmental Control Unit (ECU) electronics which use four programmable loops to control the temperatures of the case and the lid, CO 2 and O 2 within the environmental case, and airflow within the incubator. Control System Configurations ECU-H5 Controller with heat, premix gas ECU-HC Controller with CO 2 and heat ECU-HOC Controller with CO 2 and O 2 Features • Four programmable digital control loops: • Independent incubator base temperature PID control with ±0.1ºC precision • Independent incubator lid temperature PID control with ±0.1ºC precision • CO 2 digital PID control with ±0.1% precision • Airflow digital PID control from 0–900 SCCM • Electronic flow meter • Programmable alarm for out of tolerance condition on all four channels The ECU is positioned on an inverted microscope. The ECU system consist of the stagetop environment base unit and the electronic controller. Main Interface to ECU Gas Intake Temperature Sensor Gas Exhaust Gas Out Vent Gas In Port Water Reservoir Overflow Port Temperature Sensor Connector Thumb Hold (for lid removal) Lid Electrical Interface Water Reservoir Filling Port Perfusion Ports STAGETOP ENVIRONMENT (OPEN) Alarm Indicator LEDs Display Display and Configuration Buttons USB PC Interface Status Indicator LED ENVIRONMENT CONTROL UNIT (FRONT) Auxiliary Heater Connection (AirTherm or Lens Warmer) Background Gas (Air) Inlet CO2 Inlet Mixed Gas Outlet (to Chamber) Power Switch Power Cord Main Interface Cable (to STEV) Nitrogen Inlet (ECU-HOC only) ENVIRONMENT CONTROL UNIT (BACK) Lid in place Thumb Hold (for lid removal) Heated Glass Lid (Window) STAGETOP ENVIRONMENT (CLOSED) www.wpiinc.com Stagetop Environmental Control Control temperature and CO 2 in a microscope stagetop environment
Transcript of Stagetop Environmental Control
WORLD PRECISION INSTRUMENTS
08/09/2019
For short term or long term studies of living cell cultures under a microscope or for time lapse video research, a microscope stagetop incubator is essential.
Perfect for Live Cell Imaging, STEV (the stagetop environmental control system) is a heated, humidified, compact environmental case that houses your culture wells and fits on a microscope stage. The STEV can be used with 35mm well plates by simply replacing the seal on the bottom of the stagetop environment case.This system offers precision control of temperature, CO2 and O2. The system is flexible and easy to configure for a variety of experimental conditions.The system includes the Environmental Control Unit (ECU) electronics which use four programmable loops to control the temperatures of the case and the lid, CO2 and O2 within the environmental case, and airflow within the incubator.
Control System ConfigurationsECU-H5 Controller with heat, premix gas
ECU-HC Controller with CO2 and heat
ECU-HOC Controller with CO2 and O2
Features• Four programmable digital control loops:
• Independent incubator base temperature PID control with ±0.1ºC precision
• Independent incubator lid temperature PID control with ±0.1ºC precision
• CO2 digital PID control with ±0.1% precision• Airflow digital PID control from 0–900 SCCM
• Electronic flow meter• Programmable alarm for out of tolerance condition on all four
channels
The ECU is positioned on an inverted microscope. The ECU system consist of the stagetop environment base unit and the electronic controller.
Stagetop Environment - Open
Main Interface to ECU
Gas IntakeTemperature Sensor
Gas Exhaust
Gas Out Vent
Gas In Port
Water Reservoir Overflow Port
Temperature Sensor Connector
Thumb Hold (for lid removal)
Lid Electrical Interface
Water Reservoir Filling Port
Perfusion Ports
STAGETOP ENVIRONMENT (OPEN)
Alarm Indicator LEDsDisplay Display and Configuration Buttons
USB PC Interface
Status Indicator LED
Environmental Control Unit–FrontENVIRONMENT CONTROL UNIT (FRONT)
Auxiliary Heater Connection(AirTherm or Lens Warmer)
Background Gas (Air) Inlet
CO2 Inlet
Mixed Gas Outlet(to Chamber)
PowerSwitch
PowerCord
Main Interface Cable (to STEV)
Environmental Control Unit (Rear View of ECU-HCP)
Nitrogen Inlet (ECU-HOC only)
ENVIRONMENT CONTROL UNIT (BACK)
Stagetop Environment - ClosedLid in place
Thumb Hold (for lid removal)
Heated Glass Lid (Window)
STAGETOP ENVIRONMENT (CLOSED)
www.wpiinc.com
Stagetop Environmental ControlControl temperature and CO2 in a microscope stagetop environment
WORLD PRECISION INSTRUMENTS
To AuxilliaryHeater*
ECU-HOC STEV-2
Air
CO2
N2
Main Interface Cable
*Aux. heater could be a lens warmer or an AirTherm Satellite heater (for a full microscope enclosure).
Mixed Gas Flow
To AuxilliaryHeater*
ECU-HC STEV-2
Air
CO2 Main Interface Cable
*Aux. heater could be a lens warmer or an AirTherm Satellite heater (for a full microscope enclosure).
Mixed Gas Flow
ECU-H5 STEV-2
To AuxilliaryHeater*
Air/
CO2 Main Interface Cable
*Aux. heater could be a lens warmer or an AirTherm Satellite heater (for a full microscope enclosure).
Mixed Gas Flow
QUOTATION INFORMATIONWhen ordering a system, please have the following information ready.
1. Scope manufacturer/model
___________________________________________2. Stage manufacturer/model
___________________________________________ 3. Stage-piezo ◻ Yes ◻ No
Type ______________________________________4. Manipulators ◻ Yes ◻ No
Type ______________________________________5. Objective oil lens ◻ Yes ◻ No
6. Stage microfluidics ◻ Yes ◻ No
7. List specimen dishes used:
____________________________________________8. Controller model of choice: ◻ Heat 5% Premixed Tank ◻ Heat, CO2 Mixing ◻ Heat, CO2, O2
9. Imaging requirements for SI-BF100:Power ◻ 110V ◻ 240VDyes used:
___________________________________________Excitation (nm):
___________________________________________Emission (nm):
___________________________________________
SYSTEM OPTIONS• Well plate inserts• SI-BF-100 Biofluorometer for
fluorescence imaging (Includes light, filtering and timing)
SI-BF-100 Biofluorometer
WORLD PRECISION INSTRUMENTSUSA: 175 Sarasota Center Boulevard, Sarasota FL 34240-9258 USATel: (941) 371-1003 • Fax: (941) 377-5428 • E-mail: [email protected] • Internet: www.wpiinc.com
UK: 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ England • Tel: 44 (0)1462 424700 • E-mail: [email protected] Germany: Pfingstweide 16, D-61169 Friedberg (Hessen) • Tel: +49 (0)6031 67708-0 • Fax: +49 (0)6031 67708-80 • E-mail: [email protected] China & Hong Kong: Rm 18A, No8 Donfang Rd., Pudong District, Shanghai 200120 PRC • Tel: +86 688 85517 • E-mail: [email protected] Brazil: Conselheiro Nabias, 756 sala2611, Santos-São Paulo 11045-002 Brazil • E-mail: [email protected]
Stagetop Environmental ControlControl temperature and CO2 in a microscope stagetop environment
