Stability of VACUETTE Lithium Heparin Separator tubes with ... · page 1/16 Stability of VACUETTE®...
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page 1/16 Stability of VACUETTE ® Lithium Heparin Separator tubes with modified centrifugation conditions Background: Greiner-Bio-One, Austria has been selling plastic evacuated tubes (VACUETTE ® ) for venous blood collection since 1986. The anticoagulant heparin activates antithrombins, thus blocking the coagulation cascade and producing a whole blood/plasma sample making it ideal for rapid analysis and analysis of blood from patients on anticoagulant therapy. VACUETTE ® Lithium Heparin Separator tubes contain a barrier gel in the tube. The specific gravity of this material lies between the blood cells and plasma. During centrifugation the gel moves upwards providing a stable barrier separating the plasma from cells. Plasma may be aspirated directly from the collection tube, eliminating the need for manual transfer to another container for analysis. [1] Study Objective: In order to shorten turn-around-time (TAT), laboratory professionals very often centrifuge a variety of blood collection tubes under the same centrifuge settings. This study was carried out to compare two different centrifugation conditions including stability of analytes for 48 hours in order to improve laboratory efficiency by standardizing centrifugation conditions. [2,3] Study design and procedure: Venous blood was collected from 40 presumably healthy donors into four VACUETTE ® Lithium Heparin Separator tubes (456087, 13/100, 5 ml) using VACUETTE ® Safety Blood Collection Sets with Holder (item # 450085). Two different VACUETTE ® gel tubes (Sample A US P-Gel and Sample B European Gel) were included in this test. A discard tube was used to guarantee appropriate filling of the tubes. The order of collection was randomized. All tubes were inverted 8 times immediately after blood collection. Tubes were then centrifuged with a Rotanta 460R centrifuge from Hettich at two different centrifugation conditions (see Table 1). Table 1: Centrifugation Sample RCF (x g) Time Temp [°C] Sample A P-Gel 2200g 15 min 20 °C Sample A P-Gel 1800g 10 min 20 °C Sample B Gel 2200g 15 min 20 °C Sample B Gel 1800g 10 min 20 °C All sample tubes were analyzed for the components listed below at the initial time point within 2 hours of blood collection and after 48 hours on the Siemens Advia analyzer and Abbott Architect analyzer using accompanying reagents from the instrument manufacturers. Between measurements, the samples were stored in the refrigerator at 4-8 °C. The following parameters were measured: Alanine Aminotransferase (ALT) Alkaline Phosphatase (ALP) Aspartate Aminotransferase (AST) Beta-HCG Blood Urea Nitrogen (BUN) Calcium Chloride Cholesterol Cholinesterase (CHE) Creatinine
Transcript of Stability of VACUETTE Lithium Heparin Separator tubes with ... · page 1/16 Stability of VACUETTE®...
page 1/16
Stability of VACUETTE® Lithium Heparin Separator tubes with modified centrifugation conditions
Background:
Greiner-Bio-One, Austria has been selling plastic evacuated tubes (VACUETTE®) for venous blood collection since 1986. The anticoagulant heparin activates antithrombins, thus blocking the coagulation cascade and producing a whole blood/plasma sample making it ideal for rapid analysis and analysis of blood from patients on anticoagulant therapy. VACUETTE® Lithium Heparin Separator tubes contain a barrier gel in the tube. The specific gravity of this material lies between the blood cells and plasma. During centrifugation the gel moves upwards providing a stable barrier separating the plasma from cells. Plasma may be aspirated directly from the collection tube, eliminating the need for manual transfer to another container for analysis. [1] Study Objective: In order to shorten turn-around-time (TAT), laboratory professionals very often centrifuge a variety of blood collection tubes under the same centrifuge settings. This study was carried out to compare two different centrifugation conditions including stability of analytes for 48 hours in order to improve laboratory efficiency by standardizing centrifugation conditions. [2,3] Study design and procedure:
Venous blood was collected from 40 presumably healthy donors into four VACUETTE® Lithium Heparin Separator tubes (456087, 13/100, 5 ml) using VACUETTE® Safety Blood Collection Sets with Holder (item # 450085). Two different VACUETTE® gel tubes (Sample A US P-Gel and Sample B European Gel) were included in this test. A discard tube was used to guarantee appropriate filling of the tubes. The order of collection was randomized. All tubes were inverted 8 times immediately after blood collection. Tubes were then centrifuged with a Rotanta 460R centrifuge from Hettich at two different centrifugation conditions (see Table 1). Table 1: Centrifugation
Sample RCF (x g) Time Temp [°C]
Sample A P-Gel
2200g 15 min 20 °C
Sample A P-Gel
1800g 10 min 20 °C
Sample B Gel
2200g 15 min 20 °C
Sample B Gel
1800g 10 min 20 °C
All sample tubes were analyzed for the components listed below at the initial time point within 2 hours of blood collection and after 48 hours on the Siemens Advia analyzer and Abbott Architect analyzer using accompanying reagents from the instrument manufacturers. Between measurements, the samples were stored in the refrigerator at 4-8 °C. The following parameters were measured:
Alanine Aminotransferase (ALT) Alkaline Phosphatase (ALP) Aspartate Aminotransferase (AST) Beta-HCG Blood Urea Nitrogen (BUN)
Calcium Chloride Cholesterol Cholinesterase (CHE) Creatinine
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Gamma Glutamyltransferase (GGT) Glucose Iron Lactate Dehydrogenase (LDH) Magnesium Phosphate Potassium Sodium Total Bilirubin Total Protein
Triglyceride Troponin I Thyroid stimulating hormone (TSH) Urea Uric acid Vitamin B12 Free Triiodthyronin (fT3) Free Thyroxin (fT4)
Results: Comparison analysis was performed at the initial time point and at 48 hours. Statistical evaluation was performed with the T-test (α = 0.05) using StatSoft, Version 9. Clinical evaluation was based on the allowed recommendation by the German Medical Association (RIILBÄK). [4] No clinically significant differences were observed for either gel type with the two centrifugation settings of 2200g for 15 minutes and 1800g for 10 minutes at the initial time point or after 48 hours. However, Glucose values were affected by decomposition in cells after 48 hours. The use of a glycolysis inhibitor to compensate for this biochemical process would have been beneficial. Outlier testing was performed and showed that donor sample 14 (Sample A) for Alkaline Phosphatase at 2200g for 15 min was an outlier and was, therefore, excluded from statistical analysis. For Lactate Dehydrogenase, an analysis error occurred with donor sample 23 at the centrifugation setting of 2200g for 15 minutes at 48 hours (Sample B); donor sample 34 at 1800g for 10 min (Sample B) and donor sample 36 at 1800g for 10 min (Sample B) at the initial time point and 48 hours, all of which were excluded from statistical analysis. Conclusion:
In this trial, the stability of the biochemical analytes was demonstrated when using the centrifugation conditions as tested (2200g for 15 min at 20°C as well as 1800g for 10 min at 20°C) for VACUETTE® Lithium Heparin Separator tubes to optimize TAT in routine laboratories. These centrifugation conditions for VACUETTE® Lithium Heparin Separator tubes showed comparable results for the biochemical parameters tested at the initial time point and at 48 hours. Differences in results were not clinically significant.
References:
[1] Instructions for Use. Evacuated Blood Collection System. For in vitro Diagnostic Use. Rev. 13. [2] O‘Keane M. P., Evaluation of three different specimen types (serum, plasma, lithium heparin and serum gel separator) for analysis of certain analytes: clinical significance of differences in results and efficiency in use. Clin. Chem. Labor. Med. (44), 5 662-668 [3] Thomas L., Laboratory and Diagnosis. Indication and Evaluation of Laboratory Results for Medical Diagnosis. 7th edition. TH-Books. [4] Guideline from the medical association in Germany for quality assurance of laboratory tests. German Medical Journal. Vol. 105, Issue 7. 2008.
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Alanine Aminotransferase (ALT, GPT) Reference range: 0-45 U/l Sample A 0h
Alanine Aminotransferase 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
40
45
50
[U/l]
GPT 2200_15_A GPT 1800_10_A
Sample B 0h
Alanine Aminotransferase 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
40
45
50
[U/l]
GPT 2200_15_B GPT 1800_10_B
Alkaline Phosphatase: Reference range: 30-120 U/l Sample A 0h
Alkaline Phosphatase 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
[U/l]
AP 2200_15_ A AP 1800_10_A
Sample B 0h
Alkaline Phosphatase 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
[U/l]
AP 2200_15_ B AP 1800_10_B
Sample A 48h
Alanine Aminotransferase 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
40
45
50
[U/l]
GPT 2200_15_A_48 GPT 1800_10_A_48
Sample B 48h
Alanine Aminotransferase 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
40
45
50
[U/l]
GPT 2200_15_B_48 GPT 1800_10_B_48
Sample A 48h
Alkaline Phosphatase 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
U/l
AP 2200_15_ A_48 AP 1800_10_A_48
Sample B 48h
Alkaline Phosphatase 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
[U/l]
AP 2200_15_ B_48 AP 1800_10_B_48
RO04 page 4/16
Aspartate Aminotransferase (AST, GOT) Reference range: 0-35 U/l Sample A 0h
Aspartate Aminotransferase 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
60
70
[U/l]
GOT 2200_15_A GOT 1800_10_A
Sample B 0h
Aspartate Aminotransferase 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
60
70
GOT 2200_15_B GOT 1800_10_B
Beta-HCG Reference range: 0-0.1 mIE/ml Sample A 0h
Beta-HCG 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mlE
/ml]
HCG 2200_15_A HCG 1800_10_A
Sample B 0h
Beta-HCG 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mlE
/ml]
HCG 2200_15_B HCG 1800_10_B
Sample A 48h
Aspartate Aminotransferase 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
60
70
[U/l]
GOT 2200_15_A_48 GOT 1800_10_A_48
Sample B 48h
Aspartate Aminotransferase 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
60
70
GOT 2200_15_B_48 GOT 1800_10_B_48
Sample A 48h
Beta-HCG 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mlE
/ml]
HCG 2200_15_A_48 HCG 1800_10_A_48
Sample B 48h
Beta-HCG 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mlE
/ml]
HCG 2200_15_B_48 HCG 1800_10_B_48
RO04 page 5/16
Blood Urea Nitrogen (BUN) Reference range: 7-23 mg/dl Sample A 0h
Blood Urea Nitrogen (BUN) 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
12
14
16
18
20
22
24
[mg
/dl]
BUN 2200_15_A BUN 1800_10_A
Sample B 0h
Blood Urea Nitrogen (BUN) 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
12
14
16
18
20
22
24
[mg
/dl]
BUN 2200_15_B BUN 1800_10_B
Calcium Reference range: 2.1-2.7 mmol/l Sample A 0h
Calcium 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,5
1,0
1,5
2,0
2,5
3,0
[mm
ol/l
]
Ca 2200_15_A Ca 1800_10_A
Sample B 0h
Calcium 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,5
1,0
1,5
2,0
2,5
3,0
[mm
ol/l
]
Ca 2200_15_B Ca 1800_10_B
Sample A 48h
Blood Urea Nitrogen (BUN) 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
12
14
16
18
20
22
24
mg
/dl
BUN 2200_15_A_48 BUN 1800_10_A_48
Sample B 48h
Blood Urea Nitrogen (BUN) 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
12
14
16
18
20
22
24
[mg
/dl]
BUN 2200_15_B_48 BUN 1800_10_B_48
Sample A 48h
Calcium 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,5
1,0
1,5
2,0
2,5
3,0
mm
ol/l
Ca 2200_15_A_48 Ca 1800_10_A_48
Sample B 48h
Calcium 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,5
1,0
1,5
2,0
2,5
3,0
[mm
ol/l
]
Ca 2200_15_B_48 Ca 1800_10_B_48
RO04 page 6/16
Chloride Reference range: 95-105 mmol/l Sample A 0h
Chloride 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
[mm
ol/l
]
Cl 2200_15_A Cl 1800_10_A
Sample B 0h
Chloride 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
[mm
ol/l
]
Cl 2200_15_B Cl 1800_10_B
Cholesterol Reference range: 100-200 mg/dl Sample A 0h
Cholesterol 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
[mg
/dl]
Chol 2200_15_A Chol 1800_10_A
Sample B 0h
Cholesterol 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
[mg
/dl]
Chol 2200_15_B Chol 1800_10_B
Sample A 48h
Chloride 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
mm
ol/l
Cl 2200_15_A_48 Cl 1800_10_A_48
Sample B 48h
Chloride 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
[mm
ol/l
]
Cl 2200_15_B_48 Cl 1800_10_B_48
Sample A 48h
Cholesterol 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
mg
/dl
Chol 2200_15_A_48 Chol 1800_10_A_48
Sample B 48h
Cholesterol 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
[mg
/dl]
Chol 2200_15_B_48 Chol 1800_10_B_48
RO04 page 7/16
Cholesterinesterase Reference range: 4.5-11.5 kU/l Sample A 0h
Cholinesterase 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
12
14
[kU
/l]
CHE 2200_15_A CHE 1800_10_A
Sample B 0h
Cholinesterase 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
12
14
[kU
/l]
CHE 2200_15_B CHE 1800_10_B
Creatinine Reference range male: 0.55-1.17 mg/dl Sample A 0h
Creatinine 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
[mg
/dl]
Crea 2200_15_A Crea 1800_10_A
Sample B 0h
Creatinine 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
[mg
/dl]
Crea 2200_15_B Crea 1800_10_B
Sample A 48h
Cholinesterase 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
12
14
[kU
/l]
CHE 2200_15_A_48 CHE 1800_10_A_48
Sample B 48h
Cholinesterase 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
12
14
[kU
/l]
CHE 2200_15_B_48 CHE 1800_10_B_48
Sample A 48h
Creatinine 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
mg
/dl
Crea 2200_15_A_48 Crea 1800_10_A_48
Sample B 48h
Creatinine 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
[mg
/dl]
Crea 2200_15_B_48 Crea 1800_10_B_48
RO04 page 8/16
Gamma Glutamyltransferase (GGT) Reference range: 0- 55 U/l Sample A 0h
GGT 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
60
70
80
90
100
[U/l]
GGT 2200_15_A GGT 1800_10_A
Sample B 0h
GGT 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
60
70
80
90
100
[U/l]
GGT 2200_15_B GGT 1800_10_B
Glucose Reference range: 60-110 mg/dl Sample A 0h
Glucose 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
140
[mg
/dl]
Gluc 2200_15_A Gluc 1800_10_A
Sample B 0h
Glucose 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
140
[mg
/dl]
Gluc 2200_15_B Gluc 1800_10_B
Sample A 48h
GGT 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
60
70
80
90
100
U/l
GGT 2200_15_A_48 GGT 1800_10_A_48
Sample B 48h
GGT 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
60
70
80
90
100
[U/l]
GGT 2200_15_B_48 GGT 1800_10_B_48
Sample A 48h
Glucose 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
140
[mg
/dl]
Gluc 2200_15_A_48 Gluc 1800_10_A_48
Sample B 48h
Glucose 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
140
[mg
/dl]
Gluc 2200_15_B_48 Gluc 1800_10_B_48
RO04 page 9/16
Iron Reference range: 14.3-32.2 µmol/l Sample A 0h
Iron 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
[µmol/l]
Fe 2200_15_A Fe 1800_10_A
Sample B 0h
Iron 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
[µmol/l]
Fe 2200_15_B Fe 1800_10_B
Lactate Dehydrogenase Reference range: 0-248 U/l Sample A 0h
LDH 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
400
[U/l]
LDH 2200_15_A LDH 1800_10_A
Sample B 0h
LDH 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
400
[U/l]
LDH 2200_15_B LDH 1800_10_B
Sample A 48h
Iron 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
µmol/l
Fe 2200_15_A_48 Fe 1800_10_A_48
Sample B 48h
Iron 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
[µmol/l]
Fe 2200_15_B_48 Fe 1800_10_B_48
Sample A 48h
LDH 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
400
[U/l]
LDH 2200_15_A_48 LDH 1800_10_A_48
Sample B 48h
LDH 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
[U/l]
LDH 2200_15_B_48 LDH 1800_10_B_48
RO04 page 10/16
Magnesium Reference range: 0.7-1.0 mmol/l Sample A 0h
Magnesium 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
[mm
ol/l
]
Mg 2200_15_A Mg 1800_10_A
Sample B 0h
Magnesium 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
[mm
ol/l
]
Mg 2200_15_B Mg 1800_10_B
Phosphate Reference range: 0.77-1.45 mmol/l Sample A 0h
Phosphate 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[mm
ol/l
]
P 2200_15_A P 1800_10_A
Sample B 0h
Phosphate 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[mm
ol/l
]
P 2200_15_B P 1800_10_B
Sample A 48h
Magnesium 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
[mm
ol/l
]
Mg 2200_15_A_48 Mg 1800_10_A_48
Sample B 48h
Magnesium 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
Mg 2200_15_B_48 Mg 1800_10_B_48
Sample A 48h
Phosphate 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[mm
ol/l
]
P 2200_15_A_48 P 1800_10_A_48
Sample B 48h
Phosphate 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[mm
ol/l
]
P 2200_15_B_48 P 1800_10_B_48
RO04 page 11/16
Potassium Reference range: 3.5-5.3 mmol/l Sample A 0h
Potassium 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
1
2
3
4
5
6
[mm
ol/l
]
K 2200_15_A K 1800_10_A
Sample B 0h
Potassium 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
1
2
3
4
5
6
[mm
ol/l
]
K 2200_15_B K 1800_10_B
Sodium Reference range: 135-150 mmol/l Sample A 0h
Sodium 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
140
160
180
[mm
ol/l
]
Na 2200_15_A Na 1800_10_A
Sample B 0h
Sodium 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
140
160
180
[mm
ol/l
]
Na 2200_15_B Na 1800_10_B
Sample A 48h
Potassium 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
1
2
3
4
5
6
[mm
ol/l
]
K 2200_15_A_48 K 1800_10_A_48
Sample B 48h
Potassium 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
1
2
3
4
5
6
[mm
ol/l
]
K 2200_15_B_48 K 1800_10_B_48
Sample A 48h
Sodium 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
140
160
180
[mm
ol/l
]
Na 2200_15_A_48 Na 1800_10_A_48
Sample B 48h
Sodium 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
20
40
60
80
100
120
140
160
180
[mm
ol/l
]
Na 2200_15_B_48 Na 1800_10_B_48
RO04 page 12/16
Total Bilirubin Reference range: 0.1-1.2 mg/dl Sample A 0h
Total Bilirubin 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mg
/dl]
TBili 2200_15_A TBili 1800_10_A
Sample B 0h
Total Bilirubin 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mg
/dl]
TBili 2200_15_B TBili 1800_10_B
Total Protein Reference range: 6-8 g/l Sample A 0h
Total Protein 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
[g/l]
TP 2200_15_A TP 1800_10_A
Sample B 0h
Total Protein 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
[g/l]
TP 2200_15_B TP 1800_10_B
Sample A 48h
Total Bilirubin 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mm
ol/l
]
TBil i 2200_15_A_48 TBili 1800_10_A_48
Sample B 48h
Total Bil irubin 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
[mm
ol/l
]
TBili 2200_15_B_48 TBili 1800_10_B_48
Sample A 48h
Total Protein 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
[g/l]
TP 2200_15_A_48 TP 1800_10_A_48
Sample B 48h
Total Protein 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
[g/l]
TP 2200_15_B_48 TP 1800_10_B_48
RO04 page 13/16
Triglyceride Reference range: 25-180 mg/dl Sample A 0h
Triglyceride 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
[mg
/dl]
Tri 2200_15_A Tri 1800_10_A
Sample B 0h
Triglyceride 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
[mg
/dl]
Tri 2200_15_B Tri 1800_10_B
Troponin I Reference range: 0-0.028 ng/ml Sample A 0h
Troponin I 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,000
0,005
0,010
0,015
0,020
0,025
0,030
[ng
/ml]
Trop 2200_15_A Trop 1800_10_A
Sample B 0h
Troponin I 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,000
0,005
0,010
0,015
0,020
0,025
0,030
[ng
/ml]
Trop 2200_15_B Trop 1800_10_B
Sample A 48h
Triglyceride 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
[mg
/dl]
Tri 2200_15_A_48 Tri 1800_10_A_48
Sample B 48h
Triglyceride 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
50
100
150
200
250
300
350
[mg
/dl]
Tri 2200_15_B_48 Tri 1800_10_B_48
Sample A 48h
Troponin I 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,000
0,005
0,010
0,015
0,020
0,025
0,030
[ng
/ml]
Trop 2200_15_A_48 Trop 1800_10_A_48
Sample B 48h
Troponin I 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,000
0,005
0,010
0,015
0,020
0,025
0,030
[ng
/ml]
Trop 2200_15_B_48 Trop 1800_10_B_48
RO04 page 14/16
Thyroid stimulating hormone (TSH) Reference range: 0.47-3.5 µU/ml Sample A 0h
TSH 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
1
2
3
4
5
6
7
[µU/m
l]
TSH 2200_15_A TSH 1800_10_A
Sample B 0h
TSH 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
1
2
3
4
5
6
7
[µU/m
l]
TSH 2200_15_B TSH 1800_10_B
Urea Reference range: 15-50 mg/dl Sample A 0h
Urea 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
5
10
15
20
25
30
35
40
45
50
[mg
/dl]
U 2200_15_A U 1800_10_A
Sample B 0h
Urea 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
[mg
/dl]
U 2200_15_B U 1800_10_B
Sample A 48h
TSH 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
1
2
3
4
5
6
7
[µU/m
l]
TSH 2200_15_A_48 TSH 1800_10_A_48
Sample B 48h
TSH 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
1
2
3
4
5
6
7
[µU/m
l]
TSH 2200_15_B_48 TSH 1800_10_B_48
Sample A 48h
Urea 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
[mg
/dl]
U 2200_15_A_48 U 1800_10_A_48
Sample B 48h
Urea 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
10
20
30
40
50
[mg
/dl]
U 2200_15_B_48 U 1800_10_B_48
RO04 page 15/16
Uric acid Reference range male: 3.5-7.0 mg/dl Sample A 0h
Uric Acid 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
[mg
/dl]
UA 2200_15_A UA 1800_10_A
Sample B 0h
Uric Acid 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
[mg
/dl]
UA 2200_15_B UA 1800_10_B
Vitamin B12 Reference range male: 214-864 ng/l Reference range female: 182-820 ng/l Sample A 0h
Vitamin B12 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
100
200
300
400
500
600
700
800
900
[pg
/ml]
Vit B 2200_15_A Vit B 1800_10_A
Sample B 0h
Vitamin B12 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
100
200
300
400
500
600
700
800
900
[pg
/ml]
Vit B 2200_15_B Vit B 1800_10_B
Sample A 48h
Uric Acid 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
[mg
/dl]
UA 2200_15_A_48 UA 1800_10_A_48
Sample B 48h
Uric Acid 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
2
4
6
8
10
[mg
/dl]
UA 2200_15_B_48 UA 1800_10_B_48
Sample A 48h
Vitamin B12 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
100
200
300
400
500
600
700
800
900
[pg
/ml]
Vit B 2200_15_A_48 Vit B 1800_10_A_48
Sample B 48h
Vitamin B12 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410
100
200
300
400
500
600
700
800
900
[pg
/ml]
Vit B 2200_15_B_48 Vit B 1800_10_B_48
RO04 page 16/16
Free Triiodthyronin (fT3)
Reference range: 1.39-4.14 pg/ml Sample A 0h
fT3 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
4,0
[pg
/ml]
fT3 2200_15_A fT3 1800_10_A
Sample B 0h
fT3 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
4,0
[pg
/ml]
fT3 2200_15_B FT3 1800_10_B
Free Thyroxin (fT4)
Reference range: 0.75-2.0 ng/dl Sample A 0h
fT4 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[ng
/dl]
fT4 2200_15_A fT4 1800_10_A
Sample B 0h
fT4 0h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[ng
/dl]
fT4 2200_15_B fT4 1800_10_B
Sample A 48h
fT3 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
4,0
[pg
/ml]
fT3 2200_15_A_48 fT3 1800_10_A_48
Sample B 48h
fT3 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,5
1,0
1,5
2,0
2,5
3,0
3,5
4,0
[pg
/ml]
fT3 2200_15_B_48 FT3 1800_10_B_48
Sample A 48h
fT4 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[ng
/dl]
fT4 2200_15_A_48 fT4 1800_10_A_48
Sample B 48h
fT4 48h
1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 37 39 410,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
[ng
/dl]
fT4 2200_15_B_48 fT4 1800_10_B_48
