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Supplementary Table

Genotype Total Cells

Chromosome Content DTA/TC (average) 8 16-32 32-64 >64

PL (%)

DTA/TC PL (%)

DTA/TC PL (%)

DTA/TC PL (%)

DTA/TC

Ssedft 250 14.0 0.33 50.0 0.43 24.0 0.33 12.0 0.32 0.35 Ssedft/l(3)13m-281 260 4.7 0.30 59.5 0.42 25.0 0.35 13.0 0.31 0.34 Ssedft/Df(3L)ZN47 230 12.0 0.27 54.0 0.51 19.0 0.40 15.9 0.26 0.36 aSsedft 50 10.0 0.28 56.0 0.52 26.0 0.46 8.0 0.35 0.40 [RFP-HP1] Ssedft 80 20.0 0.02 47.0 0.05 25.0 0.06 8.0 0.04 0.04 UAS SseC497S Ssedft 45 24.4 0.31 44.4 0.49 20.0 0.43 11.1 0.37 0.40 UAS Sse RNAi 108 88 0.04 12 0.04 - - - - 0.04

Supplementary Table 1. Frequency of polyploidy (PL) and DTA/TC in different Sse mutant combinations or SseRNAi. Both frequencies are close to 0 in wild-type Oregon R cells (not indicated). a This value refers to Ssedft mutant cells examined for the experiments outlined in Figure 4j and 4k.