SQN Annual Water Withdrawal Updates for January 2012.

S58 120209 800 - NPDES CORRESPONDENCE

February 9, 2012

Ms. Dana Waits

State of Tennessee

Department of Environment and ConservationDivision of Water Pollution Control

Enforcement & Compliance Section6th Floor, L &C Annex401 Church Street

Nashville, Tennessee 37243-1534

Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JANUARY 2012

Enclosed is the January 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you haveany questions or need additional information, please contact Brad Love at (423) 843-6714 ofSequoyah's Environmental staff.

/ certify under penalty of lawthat this document and allattachments were prepared under mydirection orsupervision inaccordance with a system designed to assure that qualified personnelproperly gatherand evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gathering the information,the information submitted is, to the best ofmyknowledge andbelief, true, accurate, andcomplete. Iam aware that there are significant penalties for submitting false information, including thepossibilityof fine and imprisonment for knowing violations.

Sincerely,,

Jofin'XjCsfrlinSita-w^g/PresidentSequoyah Nuclear Plant

Enclosures

cc (Enclosures):Chattanooga Environmental Field OfficeDivision of Water Pollution ControlState Office Building, Suite 550540 McCallie Avenue

Chattanooga, Tennessee 37402-2013

B. E. Brickhouse, LP 5U-CJ. T. Carlin, OPS 4A-SQNG. M.Cook, OPS4A-SQNJ. A. Cross, POB 2A-SQNT.R. Markum, BR 4A-C

U.S. Nuclear Regulatory CommissionAttn: Document Control Desk

Washington, DC 20555

D. B. Nida, LP 5U-CA. A. Ray, WT11A-KG. R. Signer, WT 6A-KP.R. Simmons, POB 2B-SQNB. N. Smith (EDMS), MPB 1E-M

Tennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000

February 9, 2012

Ms. Dana Waits

State of Tennessee


Enforcement & Compliance Section6th Floor, L&C Annex401 Church Street


Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR JANUARY 2012

Enclosed is the January 2012 Discharge Monitoring Report for Sequoyah NuclearPlant. If you haveany questions or need additional information, please contact Brad Love at (423) 843-6714 ofSequoyah's Environmental staff.

/ certify under penalty of lawthat this document and allattachments were prepared under mydirection orsupervision in accordance with a systemdesignedtoassure that qualified personnelproperly gather andevaluate the information submitted. Based on myinquiry of theperson orpersons who manage the system, orthosepersons directly responsible for gathering the information,the information submitted is, to the best ofmy knowledge andbelief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibilityof fine and imprisonment forknowing violations.

:am6e President

Sequoyah Nuclear Plant

Enclosures

cc (Enclosures):Chattanooga Environmental Field OfficeDivision of Water Pollution ControlState Office Building, Suite 550540 McCallie Avenue


U.S. Nuclear Regulatory CommissionAttn: Document Control DeskWashington, DC 20555

PERMITTEE NAME/ADDRESS (Include Facility Name/Location ifDifferent)Name ___ _™A ^TOl^^^l^CLE^RPLANTAddress P.O. BOX 2000

aNJEROFRCEOPS^r^SONJ^ ~~~~~Il—lQDp^-^|^JTN_37384

FacHity__ jn^-^QyOY^NUCLEAR PLANTJxration_ Jl^lIJpiijC^UNJY

ATTN: Brad Love

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)DISCHARGE MONITORING REPORT (DMR)

From

TN0026450 101 GPERMIT NUMBER DISCHARGE NUMBER

MONITORING PERIODJfEAR MO DAY

ToYEAR MO DAY

12 01 01 12 01 31

MAJOR

(SUBR01)

F - FINAL

DIFFUSER DISCHARGE

EFFLUENT

"* NO DISCHARGE Q ***NOTE: Read instructions beforecompleting this form

PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION

TEMPERATURE, WATER DEG.CENTIGRADE

00010 1 0

EFFLUENT GROSS


00010 Z 0

INSTREAM MONITORING

TEMP. DIFF. BETWEEN SAMP. &UPSTRM DEG.C

00016 1 1

EFFLUENT GROSS

FLOW, IN CONDUIT OR THRUTREATMENT PLANT

50050 1 0

EFFLUENT GROSS

CHLORINE, TOTAL RESIDUAL

50060 1 0

EFFLUENT GROSS

TEMPERATURE - C, RATE OFCHANGE

82234 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLEMEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

********

MAXIMUM

********

********

********

********

1668

Req. Mon.DAILY MAX

********

DAILY MX

UNITS

03

MGD

62

DEG

C/HR

MINIMUM

********

********

********

********

Certify under penalty oflaw that this document and all attachments were prepared under mydirection orsupervision in accordance with asystem designed toassure that qualified personnelproperly gather and evaluate theinformation submitted. Based onmyinquiry oftheperson orpersons who manage thesystem, orthose persons directly responsible for gathering theinformation, theinformation submitted is, tothebestofmyknowledge and belief, true, accurateand complete. Iam aware that there are significant penalties for submitting false information,including the possibility of fine andimprisonment for knowing violations.

AVERAGE

********

********

********

********

********

0.029

0.1

MOAVG

********

MAXIMUM

26.0

Req. Mon.DAILY MAX

10.6

30.5

DAILY MX

DAILY MX

********

0.045

0/1

DAILY MAX

UNITS

04

DEG.C.

04

DEG.C.

04

DEG.C.

19

MG/L

Form Approved.OMB No. 2040-0004

NO.

EX

FREQUENCYOF

ANALYSIS

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

10/31

FIVE PER

WEEK

31/31

CONTI

NUOUS

SAMPLETYPE

RCORDR

CALCTD

MODELD

CALCTD

CALCTD

CALCTD

RCORDR

RCORDR

GRAB

CALCTD

CALCTD

CALCTD

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER

John T. Carlin

Sequoyah Site Vice President

TELEPHONE DATE

TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No closed mode operation. Veliger monitoring data is included as an attachment.

EPAForm 3320-1 (REV 3/99) Previous editions may be used

ice President

: OF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

423 843-7001

AREA

CODENUMBER.

12 02

YEAR MO

Page 1 of 1

08

DAY

Sample DateMean# of

ZM/m3% Settlers

Water

Temp. (°C)Sample Date

Mean# of

Asiatic

Clams/m3

Water

Temp. (°C) LOCATION SUB LOCATION

01/03/2012 14 100 26 01/03/2012 0 26 1-25-54501/10/2012 0 0 9 01/10/2011 0 9 RCW01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-123401/24/2012 0 0 13 01/24/2012 0 13 1-25-54501/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545

NOTES: %

Gravid Asiatic

Clam

COLLECTED BY

PKS

WBE

PB

WPT

CR

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different)Name _ JVA ÊQ^^HJ^CLE^R^LANTAddress.. _P.O_BOX2000_

aNJ^RQFRCEOPS^N^QN)SODp]f^.^j^jj4_37384

FacjHty__ JYA.-J3EQU0Y/W NUCLEAR PLANTJ^(ôn_JH>îypiLQOUNJY

ATTN: Brad Love


From

TN0026450 101 T

PERMIT NUMBER DISCHARGE NUMBER

MONITORING PERIOD

DAYYEAR MO DAY

To

YEAR MO

12 01 01 12 01 31

MAJOR

(SUBR01)

F - FINAL

BIOMONITORING FOR OUTFALL 101

EFFLUENT

*** NO DISCHARGE [^] ***

FormApproved.OMB No. 2040-0004

PARAMETER

XQUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPEAVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS

IC25 STATRE 7DAY CHR

CERIODAPHNIA

TRP3B • 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT********

********** Monitoring

Not Required

******** *******4r

23

PERCENTPERMITREQUIREMENT

******** ********43.2

MINIMUM

******** ******** SEMI

ANNUAL

COMPOS


PIMEPHALES

TRP6C 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

******** ********** Monitoring

Not Required

******** ********

23


******** ********43.2

MIMINUM

******** ******** SEMI

ANNUAL

COMPOS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

NAMEflTTLE PRINCIPAL EXECUTIVE OFFICER

John T. Carlln


TYPED OR PRINTED

Certify under penaltyof lawthatthis documentand allattachmentswere prepared undermydirection orsupervisionin accordancewitha system designed to assure thatqualified personnelproperly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directlyresponsible forgatheringtheinformation, the information submittedis, to the best of my knowledgeand belief,true,accurate,and complete. Iam aware that there are significantpenalties forsubmittingfalse information,including the possibility of fine and imprisonment for knowing violations.

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)Toxicity was not sampled in January 2012.

EPA Form 3320-1 (REV 3/99) Previous editions may be used

SIGNKWKE OF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

TELEPHONE

423 843-7001

AREA

CODENUMBER

DATE

12 02

YEAR MO

Page 1 of 1

08

DAY

PERMITTEE NAME/ADDRESS (IncludeFacilityName/LocaUonif Different)Name _ JVA ^EQ^^HJ^CLEAR_PLANTAddress^ _P.0_BOX2000

aNTERQFFlCEOPS^N^QN)SODDY-DAISYJTN_37384

FacjNty^ _JA^-J[EQUOY^Lgcatjpn_ jj^iypiLQOUNJY

ATTN: Brad Love


From


YEAR IMONITORING PERIOD

MO DAY

To

YEAR MO DAY

12 01 01 12 01 31

MAJOR

(SUBR01)

F - FINAL

LOW VOL. WASTE TREATMENT POND

EFFLUENT

*** NO DISCHARGE \^\ ***


PARAMETER


EX

FREQUENCYOF

ANALYSIS

SAMPLE


PH

00400 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

******** **********

**

7 ********

8 12

SU

0 15/31 GRAB

PERMITREQUIREMENT

******** ********6

MINIMUM

********9

MAXIMUM

THREE/

WEEK

GRAB

SOLIDS, TOTAL SUSPENDED

00530 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

******** **********

**

********6 6 19

MG/L

0 2/31 GRAB

PERMIT

REQUIREMENT

******** ******** ********30

MO AVG

100

DAILY MX

TWICE/

MONTH

GRAB

OIL AND GREASE

00556 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

******** **********

**

********

<6 <6 19

MG/L

0 2/31 GRAB

PERMIT

REQUIREMENT

******** ******** ********15

MOAVG

20

DAILY MX

TWICE/

MONTH

GRAB


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT1.077 1.187 03

MGD

******** ft*0rtF$rttftHtit 4f& Aft AA4f&**

**

0 31/31 RCORDR

PERMIT

REQUIREMENTReq. Mon.MOAVG

Req. MonDAILY MX

******** ******** ******** SEE

PERMIT

RCORDR

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT


John T. Carlin


TYPED OR PRINTED

Certify under penalty of lawthatthisdocument andall attachments wereprepared under mydirection orsupervision inaccordance with a systemdesigned toassurethatqualified personnelproperly gather andevaluate the information submitted. Basedon myinquiry of the person orpersonswho managethe system, or those personsdirectly responsibleforgathering theinformation, the information submitted is, to the bestof myknowledge andbelief, true, accurate,andcomplete. Iamaware thattherearesignificant penalties for submitting falseinformation,including the possibility of fineand imprisonment forknowing violations.

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)


SIGNATUrTOF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

TELEPHONE

423 843-7001

AREA

CODENUMBER

DATE

12 02

YEAR MO

Page 1 of 1

08

DAY

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name _ JVA ÊQ^^^NJJCLE^RJP^NTAddress^ _P.O_BOX2g00_ •

(LNIEROFRCEOPSIN^QN)SODj^-^|SYJlN_37384

FacJlity__ _TW_-SEQUOYAH NUCLEAR PLANJj^côn_jHîypiLCQyi£rY

ATTN: Brad Love


MAJOR

(SUBR01)

F - FINAL

RECYCLED COOLING WATER

EFFLUENT


TN0026450 110 G


From NO DISCHARGE XX

PARAMETER

MONITORING PERIOD

YEAR MO DAY

ToYEAR MO DAY

12 01 01 12 01 31

QUANTITY OR LOADING

NOTE: Read instructions before completing this form.


00010 1 0

EFFLUENT GROSS VALUE


00010 Z 0

INSTREAM MONITORING

TEMP. DIFF. BETWEEN SAMP. &

UPSTRM DEG.C

00016 1 0



50050 1 0



50060 1 0



82234 1 0


SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

********

********

********

********

********

MAXIMUM

********

********

********

********

********

Req. Mon.DAILY MX

********

DAILY MX

UNITS

03

MGD

04

DEGC

MINIMUM

********

********

********

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty of lawthatthisdocument and allattachments werepreparedundermydirection or supervision inaccordancewith a systemdesignedto assure thatqualified personnelproperlygather and evaluate the information submitted. Based on myinquiry of the person orpersons whomanage the system,or those persons directly responsibleforgatheringtheinformation, the information submittedis , to the best of myknowledge and belief, true, accurate,and complete. Iam aware that there are significant penaltiesforsubmitting false information,includingthe possibility of fine and imprisonment for knowing violations.

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No Discharge this Period


QUALITY OR CONCENTRATION

AVERAGE

********

********

********

********

0.1

MOAVG

********

MAXIMUM

REPORT

DAILY MX

30.5

DAILY MX

DAILY MX

0.1

DAILY MX

********

UNITS

04

DEGC

04

DEGC

04

DEGC

19

MG/L

NO.

EX

TELEPHONE

fee President

injure of principal executiveoffTcer or authorized agent

423 843-7001

area

CODENUMBER

FREQUENCYOF

ANALYSIS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

Five per

Week

CONTIN

UOUS

SAMPLE

TYPE

CALCTD

CALCTD

CALCTD

RCORDR

CALCTD

CALCTD

DATE

12 02 08

YEAR MO DAY

Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name __JVA -SEQUOWHJW^Address_ ^.O^BOX2pop_

(LNTCROFRCEOPSIN^QN)SQDD]L-^1SYJ1N_37384

Facjlity__JD^-^QUOY^NyCLEAR PLANJLocation. Jl^iypiLQQUNJY

ATTN: Brad Love


From

TN0026450 110 T


MONITORING PERIOD

DAYYEAR MO DAY

To

YEAR MO

12 01 01 12 01 31

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE [xx] ***NOTE: Read instructions before completing this form.


PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPE


CERIODAPHNIA

TRP3B 1 0 0



PIMEPHALES

TRP6C 1 0 0


SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

********

MAXIMUM

********

********

********

UNITS MINIMUM

43.2

MINIMUM

43.2

MINIMUM

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty of lawthatthisdocument andall attachments wereprepared undermydirection or supervision inaccordance witha system designedto assure thatqualified personnelproperly gatherand evaluatethe information submitted. Based on my inquiry of the personorpersonswho manage the system, or those persons directly responsibleforgathering theinformation, the information submitted is, to the best of my knowledgeand belief,true,accurate,and complete. Iam awarethat there are significant penalties forsubmittingfalse information,includingthe possibilityof fine and imprisonmentforknowingviolations.

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)No Discharge this Period


SIGNATURE OF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

AVERAGE

********

********

********

MAXIMUM

********

UNITS

23

PERCENT

23

PERCENT

TELEPHONE

423 843-7001

AREA

CODENUMBER

SEMI

ANNUAL

COMPOS

SEMI

ANNUAL

COMPOS

DATE

12 02 08

YEAR MO DAY

Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation ifDifferent)Name _ _™A ^SEQl^^^NJJCÊ^R^l^NTAddress__ _P.O_BOX2poo^

aNTCRQFRÔPS^r^SQN) ~~SODr^-^JSYJTN_37384 ;

Facjljty__ _JW_-JEQUOYA^NUCLEAR PLANTLocaloi]_Jhi^lLIPiL(ÛJ£rY

ATTN: Brad Love


From


MONITORING PERIODYEAR MO DAY

ToYEAR MO DAY

12 01 01 12 01 31


MAJOR

(SUBR01)

F - FINAL

WASTEWATER & STORM WATER

EFFLUENT

*** NO DISCHARGE ~XX

PARAMETER QUANTITY OR LOADINGNOTE: Read instructions before completing thisform.

QUALITYOR CONCENTRATION NO.

EX

FREQUENCYOF

ANALYSIS

SAMPLETYPE

OXYGEN, DISSOLVED (DO)

00300 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

SOLIDS, SETTLEABLE

00545 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

Req. Mon.MOAVG

MAXIMUM

********

********

********

Req. Mon.DAILY MX

UNITS

03

MGD

MINIMUM

2

MINIMUM

********

********

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflaw that this document and all attachments were prepared under mydirection orsupervision in accordance with asystem designed toassure that qualified personnelproperly gather and evaluate theinformation submitted. Based onmy inquiry oftheperson orpersons who manage thesystem, orthose persons directly responsible for gathering theinformation, theinformation submitted is , tothebestofmyknowledge and belief, true, accurateand complete. Iam aware that there are significant penalties for submitting false informationincluding the possibility of fine andimprisonment for knowing violations.

John T. Carlln


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall

EPAForm 3320-1(REV 3/99) Previous editions may be used

AVERAGE

********

********

********

********

MAXIMUM

********

100

DAILY MX

DAILY MX

********

UNITS

19

MG/L

19

MG/L

25

ML/L

TWICE/

WEEK

TWICE/

WEEK

ONCE/

MONTH

ONCE/

BATCH

Page 1 of 1

GRAB

GRAB

GRAB

ESTIMA

REVIEW/CONCURRENCE SHEET

DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - January 2012 DMR

ORGANIZATION: Environmental

DOCUMENT PREPARED BY: Jennifer Grubb

DATE: 2/6/2012

CONCURRENCES

Name R

V

C

N

Signature - Comment Date

J.L Grubb X 6X^-^2^r-— &/l 111,B.M. Love X ^^¥-~^k^^ i*lihz~J.A. Cross X /\/AAtLOr(>t\//sW+> 2-lrhy-

INSTRUCTIONS: Originator will determine the review/concurrence assignment.

REVIEW: Examine technical content and commitments made. Areview (RV) should confirm the truth and accuracy of factual statements andindicate agreement with commitments made which are applicable to thereviewer's organization.

CONCURRENCE: Indication of agreement with the document as a whole.Concurrence (CN) signifies that the document is responsive to theintended purpose, logical in construction, and clear in meaning in the eyesof the recipient. A concurrence signature indicates that the individualwould be willing to sign the document for the agency.


March 8, 2012

Ms. Dana Waits

State of Tennessee




Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR FEBRUARY 2012

Enclosed is the February 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Ifyou haveany questions or need additional information, please contact Brad Love at (423) 843-6714 ofSequoyah's Environmental staff.

In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcomingactivities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18refueling outage scheduled to begin in October 2012. This project has commenced and to date therehas been zero discharge of process water. Sequoyah will continue to update TDEC on this processand report any discharges and associated monitoring in future DMR's.

/ certify under penalty of law that this document and all attachments were prepared under mydirection or supervision in accordance witha system designed to assure that qualified personnelproperly gather and evaluate the information submitted. Based on my inquiryof the person orpersons who manage the system, or those persons directlyresponsible for gathering the information,the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibilityof fine and imprisonment for knowing violations.

Sincerely

;ident


Enclosures

cc (Enclosures):Chattanooga Environmental Field OfficeDivision of Water Pollution Control

State Office Building, Suite 550540 McCallie Avenue


B. E. Brickhouse, BR 4A-CJ. T. Carlin, OPS 4A-SQNG. M. Cook, OPS 4A-SQNJ. A. Cross, POB 2A-SQNT.R. Markum, BR 4A-C



D. B. Nida, BR4A-CA. A. Ray, WT11A-KG. R. Signer, WT 6A-KP.R. Simmons, POB 2B-SQNB. N. Smith (EDMS), MPB 1E-M


March 8, 2012

Ms. Dana Waits

State of Tennessee




Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR FEBRUARY 2012

Enclosed is the February 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you haveany questions or need additional information, please contact Brad Love at (423) 843-6714 ofSequoyah's Environmental staff.

In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcomingactivities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18refueling outage scheduled to begin in October 2012. This project has commenced and to date therehas been zero discharge of process water. Sequoyah will continue to update TDEC on this processand report any discharges and associated monitoring in future DMR's.

/ certify under penalty of law that this document and all attachments were prepared under mydirection or supervision in accordance with a system designed to assure that qualified personnelproperly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gathering the information,the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibilityof fine and imprisonment for knowing violations.

Sincj

froPresident


Enclosures






PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)

Name __JVA^EQ^^m^CLEARPLANT^<^SS__P.a_BOX2p00_

UNTERQFRCEOPS^N^QN)SOT^-DAJSYJ1N_37384

Facjnty_ JVA; SE^OYAJ±NUCLEAR PLANJLgcatiOTL. Jd^lUPILQQUJNJY

ATTN: Brad Love


From

TN0026450 101 G



To

YEAR MO DAY

12 02 01 12 02 29

MAJOR

(SUBR01)

F - FINAL

DIFFUSER DISCHARGE

EFFLUENT

*** NO DISCHARGE [^ ***NOTE: Read instructions before completing this form



00010 1 0

EFFLUENT GROSS


00010 Z 0

INSTREAM MONITORING


UPSTRM DEG.C

00016 1 1

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS


50060 1 0

EFFLUENT GROSS


82234 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

MAXIMUM

********

********

********

1486

Req. Mon.DAILY MAX

DAILY MX

UNITS

03

MGD

62

DEG

C/HR

MINIMUM

********

********

AVERAGE

********

********

WWW'ftJt$t'lt'§t

********

0.034

0.1

MOAVG

• ********

MAXIMUM

29.4

Req. Mori.DAILY MAX

11.7

30.5

DAILY MX

5

DAILY MX

********

0.044

0.1

DAILY MAX

UNITS

04

DEG.C.

04

DEG.C.

04

DEG.C.

19

MG/L


NO.

EX

FREQUENCYOF

ANALYSIS

29/29

CONTI

NUOUS

29/29

CONTI

NUOUS

29/29

CONTI

NUOUS

29/29

CONTI

NUOUS

8/29

FIVE PER

WEEK

29/29

CONTI

NUOUS

SAMPLETYPE

RCORDR

CALCTD

MODELD

CALCTD

CALCTD

CALCTD

RCORDR

RCORDR

GRAB

CALCTD

CALCTD

CALCTD

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertifyunder penalty of lawthat this document and all attachments were prepared under mydirectionor supervision in accordance witha system designed to assure that qualifiedpersonnelproperlygather and evaluate the information submitted. Based on my inquiryof the person orpersons who manage the system, or those persons directlyresponsible for gathering theinformation, the information submitted is, to the best of myknowledgeand belief, true, accurate,and complete. I am aware that there are significantpenalties for submittingfalse information,including the possibilityof fine and imprisonmentfor knowingviolations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here)No dosed mode operation. Veiiger monitoring data is included as an attachment.


SIGNATORE OF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

423 843-7001

AREA

CODENUMBER

12 03

YEAR MO

Page 1 of 1

08

DAY

Sample DateMean# of

ZM/m3% Settlers

Water

Temp. (*C)Sample Date

Mean* of

Asiatic

Clams/m3

Water

Temp, fC)LOCATION SUB LOCATION

NOTES: %

Gravid Asiatic

Clam

COLLECTED BY

01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name _ JVA ÊQ^^HjgjUCL^FÎ^^p^^^.O^BOXJOOO.

aNIERQF^CEOPS^rlSQN) :SODpYrDAlSYJLN_37384

Facj!lty_ JfW.- SEQUOY^NUCLEAR PLANTi^(Mojl_Ji>^l!JPiL(ÛNJY

ATTN: Brad Love


From

TN0026450 101 TPERMIT NUMBER DISCHARGE NUMBER


ToYEAR MO DAY

12 02 01 12 02 29

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE Q ***


PARAMETER


EX

FREQUENCYOF

ANALYSIS

SAMPLETYPE

AVERAGE MAXIMUM UNITS MINIMUM AVERAGE MAXIMUM UNITS


CERIODAPHNIA

TRP3B 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT** Monitoring

Not Required

******** ********

23

PERCENTPERMIT

REQUIREMENT

******** ********43.2

MINIMUM

******** ******** SEMI

ANNUAL

COMPOS


PIMEPHALES

TRP6C 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

********** Monitoring

Not Required

******** ********

23

PERCENTPERMIT

REQUIREMENT

******** ********43.2

MIMINUM

******** ******** SEMI

ANNUAL

COMPOS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

—


John T. Carlln


TYPED OR PRINTED

Certify under penalty oflaw thatthisdocument andallattachments were prepared under mydirection orsupervision Inaccordance with a system designed toassurethatqualified personnelproperly gatherandevaluate the information submitted. Based on my inquiry oftheperson orpersonswhomanagethe system, or those personsdirectly responsible forgathering theinformation, theinformation submitted, is, tothebestofmy knowledge andbelief, true, accurate,andcomplete. Iamaware thattherearesignificant penalties forsubmitting false information,including the possibility offineand imprisonment forknowing violations.

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)Toxicity was not sampled in February 2012.


AM4Uice President

OF PRINCIPAL EXECUTIVEOR AUTHORIZED AGENT

TELEPHONE

423 843-7001

AREA

CODENUMBER

DATE

12 03

YEAR MO

Page 1 of 1

08

DAY

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name _ _™A^rai^^^N^CIJ^RJ|l^NTMdres^ J^OJ3OX_20Q0_ ; .

(INJETOmCEOPS^NbSQN) <SQDDX-^1SYJ:NJ7384

Facjl|ty_ JT^-^QyOY/W NUCLEAR PLANJi^(^oji_Jl^lUPiLCQyi£rY_.

ATTN: Brad Love


From


MONITORING PERIODYEAR MO I DAY YEAR MO DAY

12 02 | 01 To 12 02 29

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE {^\ ***NOTE: Read instructions before completingthis form.


PH

00400 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

OIL AND GREASE

00556 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

wwwwwwww

1.041

Req. Mon.MOAVG

MAXIMUM

********

&Aft4t4tftft&

********

1.246

Req. MonDAILY MX

UNITS

03

MGD

MINIMUM

6

MINIMUM

AVERAGE

********

********

8

30

MOAVG<6

15

MOAVG********

MAXIMUM

MAXIMUM

8

100

DAILY MX

<6

20

DAILY MX

UNITS

12

SU

19

MG/L

19

MG/L


NO.

EX

FREQUENCYOF

ANALYSIS

13/29

THREE/

WEEK

2/29

TWICB

MONTH

2/29

TWICE/

MONTH

29/29

SEE

PERMIT

SAMPLETYPE

GRAB

GRAB

GRAB

GRAB

GRAB

GRAB

RCORDR

RCORDR

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflaw thatthis document andall attachments were prepared under mydirection orsupervision inaccordance with a system designed toassure that qualified persomproperly gatherandevaluate the information submitted. Based onmy inquiry oftheperson orpersonswhomanagethe system, or thosepersonsdirectly responsible forgathering theInformation, theinformation submitted is, tothebestofmy knowledge andbelief, true, accurate,andcomplete. Iamaware thatthereare significant penalties forsubmitting falseinformation,including the possibility offineand imprisonment forknowing violations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANY VIOLATIONS (Reference all attachments here)


ice President

RE OF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

423 843-7001

AREA

CODENUMBER

12

YEAR

03

MO

Page 1 of 1

08

DAY

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different)

Name __ J™* ^MUOYÎWCÊAÛOT^tosS_^.O^BOX200p_

aNTEEQFFJCE OPSJ5N45QN)SODDX- DAISYJTL37384

i3CjHty_J3^-^QyOYy^J^<ôn_JH>ÎIJPJ^TOUNJY

ATTN: Brad Love


From

TN0026450 110 G



To

YEAR MO DAY

12 02 01 12 02 29

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE [xx] ***NOTE: Read Instructions before completing this form.



00010 1 0



00010 Z 0

INSTREAM MONITORING


UPSTRM DEG.C

00016 1 0



50050 1 0



50060 1 0



82234 1 0


SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE MAXIMUM

********

********

Req. Mon.DAILY MX

********

DAILY MX

UNITS

03

MGD

04

DEGC

MINIMUM

********

********

********

AVERAGE

********

********

********

********

* 0.1

MOAVG********

********

MAXIMUM

REPORT

DAILY MX

30.5

DAILY MX

5

DAILY MX********

0.1

DAILY MX

UNITS

04

DEGC

04

DEGC

04

DEGC

19

MG/L


NO.

EX

FREQUENCYOF

ANALYSIS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

Five per

Week

CONTIN

UOUS

SAMPLETYPE

CALCTD

CALCTD

CALCTD

RCORDR

CALCTD

CALCTD

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify underpenaltyof lawthat thisdocument and allattachmentswere preparedundermydirection orsupervision inaccordance with a system designed toassurethatqualified personnelproperlygather and evaluate the information submitted. Based on myinquiryof the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the information submittedis, to the best of myknowledgeand belief,true, accurate,and complete. Iam aware that there are significantpenalties for submitting false information,includingthe possibilityof fine and imprisonmentfor knowingviolations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference allattachments here)No Discharge this Period


fe Vice President

JRE OF PRINCIPAL EXECUTIVEFICER OR AUTHORIZED AGENT

423 843-7001

AREA

CODENUMBER

12 03

YEAR MO

Page 1 of 1

08

DAY


Name _J™*ÊQ^^|^r^(ÊARPLANTAddress^ _P.a.BOX2000.

aNTEraFRCEOPS^N^QN)S0Dpx-^]SYjw^7384

£acj!jty_ JVA.- SEQUOYAllNyCLEAR PLANJMcôn_JlAMypJLQOyi£rY

ATTN: Brad Love


From

TN0026450 110 T



To

YEAR I MO DAY

12 02 01 12 | 02 29

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE XX


NOTE: Read instructions before completing this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX

FREQUENCYOF

ANALYSIS

SAMPLETYPE


CERIODAPHNIA

TRP3B 1 0 0



PIMEPHALES

TRP6C 1 0 0


SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

MAXIMUM

********

********

UNITS MINIMUM

43.2

MINIMUM

43.2

MINIMUM

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty of lawthat thisdocument and allattachments werepreparedundermydirection orsupervision inaccordancewith a systemdesignedto assure thatqualified personnelproperly gatherand evaluate the information submitted. Based on myinquiry of the personorpersons whomanage the system, or those persons directly responsiblefor gatheringtheinformation, the information submitted is, to the best ofmyknowledge and belief, true,accurate,and complete. Iam aware that there are significant penaltiesforsubmitting false information,includingthe possibilityof fine and imprisonmentfor knowingviolations.

John T. Carlin


TYPED OR PRINTED



SIGNKIWRE OF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

AVERAGE MAXIMUM

********

********

UNITS

23

PERCENT

23

PERCENT

AREA

CODE

843-7001

NUMBER

SEMI

ANNUAL

SEMI

ANNUAL

COMPOS

COMPOS

YEAR MO DAY

Page 1 of 1


Name _ _™A ÊQl^YAHJW^^q^s^^P.O^BOXJOOO.

aNTCRQFFICEOPSÎ^SQN)SOTr^-ÂISYJlN_37384

Facjlity_ JVA_-SEQUOY/W NUCLEAR PLANJj^<ôn_jH>WiljpiL^

ATTN: Brad Love


From

TN0026450

PERMIT NUMBER

118 G

DISCHARGE NUMBER


To

YEAR I MO DAY

12 02 01 12 | 02 29

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE XX


NOTE: Read instructions before completing this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX

FREQUENCYOF

ANALYSIS

SAMPLETYPE


00300 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

SOLIDS, SETTLEABLE

00545 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

Req. Mon.MOAVG

MAXIMUM

itititit'itititit

w&kkkxx&

Req. Mon.DAILY MX

UNITS

03

MGD

MINIMUM

2

MINIMUM

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenalty of lawthatthisdocument andall attachments wereprepared undermydirection or supervision inaccordance witha system designedto assurethatqualified personnelproperly gather andevaluate the information submitted. Basedon myinquiry of the person orpersonswhomanagethe system, orthose personsdirectly responsible forgathering theinformation, the information submitted is, to the best of myknowledge andbelief, true, accurate,andcomplete. Iamaware thattherearesignificant penalties for submitting falseinformation,includingthe possibility of fine and imprisonmentforknowingviolations.

AVERAGE

********

********

********

John T. Carlin

Sequoyah Site Vice PresidentSIGNATftSEJOTPRINCIPAL EXECUTIVE

OFFICER OR AUTHORIZED AGENTTYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here)During this reporting period, there has been no flow from the Dredge Pond otherthanthat resulting from rainfall.


MAXIMUM

********

********

100

DAILY MX

1

DAILY MX

********

resident

UNITS

19

MG/L

19

MG/L

25

ML/L

TELEPHONE

843-7001

AREA

CODENUMBER

TWICE/

WEEK

TWICE/

WEEK

ONCE/

MONTH

ONCE/

BATCH

GRAB

GRAB

GRAB

ESTIMA

DATE

12 03 08

YEAR MO DAY

Page 1 of 1


DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - February 2012 DMR



DATE: 3/7/2012

CONCURRENCES

Name R

V

C

N


J.L Grubb X S• £c<&h U^-^xUom 3h|itB.M. Love X ^^~~\rC~Z>-~- 3f*l'^<lJ> —tjz^-—J.A. Cross X IJA*Jfa<^ zlsrlt?-

INSTRUCTIONS: Originatorwill determine the review/concurrence assignment.




April 6, 2012

Ms. Dana Waits

State of Tennessee




Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MARCH 2012

Enclosed is the March 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you haveany questions or need additional information, please contact Brad Love at (423) 843-6714 ofSequoyah's Environmental staff.

In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcomingactivities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle 18refueling outage scheduled to begin in October 2012. This project is on going and to date there hasbeen zero discharge of process water. Sequoyah will continue to update TDEC on this process andreport any discharges and associated monitoring in future DMR's.

/ certify under penalty of law that this document and all attachments were prepared under mydirection or supervision in accordance with a system designed to assure that qualified personnelproperly gather and evaluate the information submitted. Based on my inquiryof the person orpersons who manage the system, or those persons directlyresponsible for gatheringthe information,the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibilityof fine and imprisonment for knowing violations.

Sincerely,,-

^residentSequoyah Nuclear Plant

Enclosures

cc (Enclosures):Chattanooga Environmental Field OfficeDivision of Water Pollution ControlState Office Building, Suite 550540 McCallie AvenueChattanooga, Tennessee 37402-2013

S.D.Booker, MOB 1F-WBNB. E. Brickhouse, BR 4A-CJ. T. Carlin, OPS 4A-SQNG.M.Cook, OPS 4A-SQNJ.A.Cross, POB2A-SQNT.R. Markum, BR4A-C

U.S. Nuclear Regulatory CommissionAttn: Document Control DeskWashington, DC 20555

D. B. Nida, BR 4A-CA. A. Ray,WT11A-KG.R. Signer, WT6A-KP.R. Simmons, POB 2B-SQNB. N. Smith (EDMS), MPB 1E-M

MTennessee Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000

April 6, 2012

Ms. Dana Waits

State of Tennessee




Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR MARCH 2012

Enclosed is the March 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Ifyou haveany questions or need additional information, please contact Brad Love at (423) 843-6714 ofSequoyah's Environmental staff.

In a letter to Mr. Vojin Janjic dated December 21, 2011 Sequoyah notified TDEC of upcomingactivities for the installation of pilings into bedrock to support a crane duringthe Unit 2 Cycle 18refueling outage scheduled to begin in October 2012. This project is on going and to date there hasbeen zero discharge of process water. Sequoyah will continue to update TDEC on this process andreport any discharges and associated monitoring in future DMR's.

/ certify under penalty of law that this document and allattachments were prepared under mydirection or supervision in accordance with a system designed to assure that qualified personnelproperly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gathering the information,the information submitted is, to the best of my knowledge and belief, true, accurate, and complete. Iam aware that there are significant penalties for submitting false information, including the possibilityof fine and imprisonment for knowing violations.

Sincerely,

;arlin

be President

foyah Nuclear Plant

Enclosures






PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name __JVA -SEQ^^^r^CL^^l^NTAddress_ J^QU3QX2gog_

QNTEROFRCE OPS^N^QN)SQDDY.-DA]SYJ1N_37384

Facji|ty__ jn^-^QyOY^NUCLEAR PLANJM(^ojL_JH>^IIJPiLCQUi£rY

ATTN: Brad Love


From



To

YEAR MO DAY

12 03 01 12 03 31

MAJOR

(SUBR01)

F - FINAL

DIFFUSER DISCHARGE

EFFLUENT

*** NO DISCHARGE \^J ***NOTE: Read instructions before completingthis form.

Form Approved.OMB No.2040-0004


EX

FREQUENCYOF

ANALYSIS

SAMPLE



00010 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

31.3

Req. Mon.DAILY MAX

04

DEG.C.


00010 Z 0

INSTREAM MONITORING

SAMPLE

MEASUREMENT

******** ********

18.9


UPSTRM DEG.C

00016 1 1

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS


50060 1 0

EFFLUENT GROSS


82234 1 0

EFFLUENT GROSS

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

WWWftW'9CW'W

1450

Req. Mon.DAILY MAX

wwAAxAAIf

DAILY MX

03

MGD

62

DEG

C/HR

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law thatthisdocument andall attachments were prepared under mydirection orsupervision inaccordance with a system designed toassure that qualified personnt

John T. Carlin


TYPED OR PRINTED

properly gather andevaluate the information submitted. Basedon my inquiry of the person orpersonswhomanagethe system, orthose personsdirectly responsible forgathering theinformation, the information submitted is, to thebestofmyknowledge andbelief, true, accurate,and complete. Iamawarethatthereare significant penalties forsubmitting false information,includingthe possibility of fine and imprisonmentforknowingviolations.

COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here)No closed mode operation. Veliger monitoring data is included as an attachment.

********

********

0.028

0.1

MOAVG********

********

30.5

DAILY MX

DAILY MX

0.057

0.1

DAILY MAX

04

DEG.C.

04

DEG.C.

19

MG/L

TELEPHONE


423 843-7001

AREA

CODENUMBER

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

20/31

FIVE PER

WEEK

31/31

CONTI

NUOUS

RCORDR

CALCTD

MODELD

CALCTD

CALCTD

CALCTD

RCORDR

RCORDR

GRAB

CALCTD

CALCTD

CALCTD

DATE

12 04 05

YEAR MO DAY

EPA Form 3320-1 (REV 3/99) Previous editions may be usedPage 1 of 1

Sample DateMean# of

ZM/m3% Settlers

Water

Temp. fC)Sample Date

Mean# of

Asiatic

Clams/m3

Water

Temp. (°C) LOCATION SUB LOCATIONNOTES: %

Gravid Asiatic

Clam

COLLECTED BY

01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE


Name __™A ^TOl^^^l^C^E^R^LANTAddress_ _P.a.BOX2000.

ONJEROFRCEOPS^IISQN)SQD[^-^1SY1JN_37384

Facjljty__ J[\^-^QyOY/^NUCLEAR PLANT_LgcatiQD_JiAMypjLQQUNJY

ATTN: Brad Love


From

TN0026450 101 T



To

YEAR MO DAY

12 03 01 12 03 31

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE \~\ ***NOTE: Read Instructions before completing this form.


PARAMETER

x-QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX

FREQUENCYOF

ANALYSIS

SAMPLE



CERIODAPHNIA

TRP3B 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

******** ********** Monitoring

Not Required

******** ********

23

PERCENTPERMIT

REQUIREMENT

******** ********43.2

MINIMUM

******** ******** SEMI

ANNUAL

COMPOS


PIMEPHALES

TRP6C 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

******** ********** Monitoring

Not Required

******** ********

23

PERCENTPERMIT

REQUIREMENT

******** ********43.2

MIMINUM

******** ******** SEMI

ANNUAL

COMPOS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT


John T. Carlin


TYPED OR PRINTED

Certify underpenalty of lawthatthisdocument andallattachments were prepared undermydirection orsupervision inaccordance witha system designedto assure thatqualified personnelproperly gatherand evaluatethe information submitted. Based on my inquiry of the personorpersonswho managethe system, orthose personsdirectly responsibleforgathering theinformation, the information submittedis, to the best of my knowledgeand belief,true,accurate,and complete. Iam awarethat there are significant penaltiesforsubmitting false information,including the possibilityof fine and imprisonment forknowingviolations.

COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here)Toxicity was not sampled in March 2012.


SIGNATURE OF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT AREA

CODENUMBER YEAR MO DAY

Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name _ JV* ÊQ^\^H_NJK^ÎÎÎ£rAddress_ _£.O_B0X2p00_

aNTCRQFRCEOPS^N^QN)SODT^-r^lSY1JN_37384

FacHity___TVA_-SEQUOYAJ^NUCLEAR PLANJLocatLqn_ JiAMIJPiLCÛNJY

ATTN: Brad Love


From

TN0026450 103 G



To

YEAR MO DAY

12 03 01 12 03 31

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE Q ***NOTE: Read instructions before completing this form.


PH

00400 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

OIL AND GREASE

00556 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

1.088

Req. Mon.MOAVG

MAXIMUM

"kfckiUcitlcIt

********

********

1.214

Req. MonDAILY MX

UNITS

03

MGD

MINIMUM

6

MINIMUM

********

AVERAGE

********

********

8

30

MOAVG

<6

15

MOAVG

MAXIMUM

8

9

MAXIMUM

8

100

DAILY MX

<6

20

DAILY MX

UNITS

12

SU

19

MG/L

19

MG/L


NO.

EX

FREQUENCYOF

ANALYSIS

15/31

THREE/

WEEK

2/31

TWICE/

MONTH

2/31

TWICE/

MONTH

31/31

SEE

PERMIT

SAMPLE

TYPE

GRAB

GRAB

GRAB

GRAB

GRAB

GRAB

RCORDR

RCORDR

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify underpenaltyof lawthat this document and allattachments were preparedundermydirection or supervision inaccordancewith a systemdesignedto assure thatqualified personnelproperlygather and evaluate the information submitted. Basedon myinquiry of the personorpersons whomanagethe system,or those persons directly responsibleforgatheringtheinformation, the information submittedis, to the best ofmyknowledge and belief, true, accurate,and complete.Iam aware that there are significant penaltiesforsubmitting false information,includingthe possibility of fine and imprisonment for knowing violations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here)


Sfuoyan Site Vice President


423 843-7001

AREA

CODENUMBER

12 04

YEAR MO

Page 1 of 1

05

DAY


Name __™Al?EQUO^HJ^CLE^R^^NTAddress_ JLQJ*OX_2000.

aNTERQFRCEOPS^Nj^QN)SODC^-MiSYJlN_37384__.

Facjjjty_ JH^-J^QUOY/W MCLEAR PLANJJxcation_Jl>^IUPiLQQUjnY

ATTN: Brad Love


From



ToYEAR MO DAY

12 03 01 12 03 31

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE [xx] ***NOTE: Read instructions beforecompleting this form.



EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPEAVERAGE MAXIMUM UNITS MINIMUM


00010 1 0



00010 Z 0

INSTREAM MONITORING

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

******** ********

********


UPSTRM DEG.C

00016 1 0


SAMPLE

MEASUREMENT

WWW'&ftJt'WW


50050 1 0


PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

******** ********

Req. Mon.DAILY MX

03

MGD


50060 1 0


PERMIT

REQUIREMENT

******** ******** ********


82234 1 0


SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

********

04

DEGC

DAILY MX

NAMEmTLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty oflaw that this document and all attachments were prepared under mydirection orsupervision inaccordance with a system designed toassure thatqualified personnelproperly gather andevaluate the Information submitted. Based onmyinquiry ofthe person orpersons whomanage the system, orthosepersons directly responsible for gathering theinformation, the information submitted is, tothe best ofmy knowledge and belief, true, accurate?andcomplete. Iamaware thatthere aresignificant penalties for submitting false information,including the possibility of fineand imprisonment forknowing violations.

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANY VIOLATIONS

No Discharge this Period(Reference all attachments here)



AVERAGE

********

********

********

0.1

MOAVGwwwwww $fw

MAXIMUM

REPORT

DAILYMX

30.5

DAILYMX

DAILYMX********

********

0.1

DAILY MX********

********

UNITS

04

DEGC

04

DEGC

04

DEGC

19

MG/L

TELEPHONE

ice President423 843-7001

AREA

CODENUMBER

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

Five per

Week

CONTIN

UOUS

CALCTD

CALCTD

CALCTD

RCORDR

CALCTD

CALCTD

DATE

12 04 05

YEAR MO DAY

Page 1 of 1


Name __™A ^EQ^^m^CLE^^LANTjM<^ss__P.01_BOX2gop_

QNTEROFFiCEOPS^rtSON)SOTDY.-DAjSYJT07384_

Facjijty_ JfVA.- SEQyOYi^NUCLEAR PLANJLocatLqn_ JH/^iypiLCOUNJY..

ATTN: Brad Love


From

TN0026450 110 TPERMIT NUMBER DISCHARGE NUMBER


ToYEAR MO DAY

12 03 01 12 03 31

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE [xx] ***NOTE: Read instructions before completingthis form.



EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPE


CERIODAPHNIA

TRP3B 1 0 0



PIMEPHALES

TRP6C 1 0 0


SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE MAXIMUM

********

********

UNITS MINIMUM

43.2

MINIMUM

43.2

MINIMUM

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty oflaw thatthisdocument andallattachments wereprepared under mydirection orsupervision inaccordance with a system designed toassurethatqualified personnelproperly gatherand evaluatethe information submitted. Basedon myinquiry ofthe personorpersonswhomanagethe system,or those personsdirectly responsible forgathering theinformation, the information submitted is, to the bestofmyknowledge and belief, true,accurate,and complete. Iam awarethat there are significant penaltiesforsubmitting false information,including the possibility offine and imprisonment forknowing violations.

John T. Carlln


TYPED OR PRINTED

COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here)No Discharge this Period


AVERAGE


MAXIMUM

********

UNITS

23

PERCENT

23

PERCENT

AREA

CODE

843-7001

NUMBER

SEMI

ANNUAL

SEMI

ANNUAL

COMPOS

COMPOS

YEAR MO DAY

Page 1 of 1

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name _ JVA -^(MI^^^I^C^^r^LANTAddress^ Jl.OJ|gX2p00_

(LNTEROFRCEOPSIN^QN)SODDX-DA1SYJ7137384

Facji|ty_ J^-^QUOYM IWCLEAR PLANJi^catjon_ Ji^iyPILQOUNJY

ATTN: Brad Love


From



ToYEAR MO DAY

12 03 01 12 03 31


MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE XX

NOTE: Read instructions beforecompleting this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPE


00300 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

SOLIDS, SETTLEABLE

00545 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLEMEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

********

********

Req. Mon.MOAVG

MAXIMUM

********

********

********

Req. Mon.DAILYMX

UNITS

03

MGD

MINIMUM

2

MINIMUM

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty of law thatthisdocument andall attachments were prepared under mydirection orsupervision inaccordance with a systemdesigned to assurethatqualified personnelproperly gather, andevaluate the information submitted. Based onmyinquiry ofthe person orpersonswhomanage the system,orthose persons directly responsible for gathering theinformation, the information submitted is, to the bestofmyknowledge andbelief, true, accurate,andcomplete. Iamaware thattherearesignificant penalties for submitting false information,including the possibility of fineand imprisonment forknowing violations.

John T. Carlin


TYPED OR PRINTED

COMMENTS ANDEXPLANATION OFANYVIOLATIONS (Reference allattachments here)During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.


AVERAGE

********

********

********

MAXIMUM

********

100

DAILYMX

DAILYMX

********

UNITS

19

MG/L

19

MG/L

25

ML/L

TELEPHONE

_ iVice PresidentSIGNATUREOF PRINCIPAL EXECUTIVE

OFFICER OR AUTHORIZED AGENT

423 843-7001

AREA

CODENUMBER

TWICE/

WEEK

TWICE/

WEEK

ONCE/

MONTH

ONCE/

BATCH

GRAB

GRAB

GRAB

ESTIMA

DATE

12 04 05

YEAR MO DAY

Page 1 of 1


DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - March 2012 DMR



DATE: 4/5/2012

Name

J.L Grubb

B.M. Love

J.A. Cross

R

V

C

N

CONCURRENCES


4 lei12.-Hid IZ.

VI*ll*





May 10, 2012

Ms. Dana Waits

State of Tennessee




Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR APRIL 2012

Enclosed is the April 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. Ifyou have anyquestions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah'sEnvironmental staff.



Sincerely

Site-vice PresidentSequoyan Nuclear Plant

Enclosures




S.D. Booker, MOB 1F-WBNB. E. Brickhouse, BR 4A-CJ.T. Carlin, OPS 4A-SQNG. M. Cook, OPS 4A-SQNJ. A. Cross, POB 2A-SQNT.R. Markum, BR 4A-C



D. B. Nida, BR4A-CA. A. Ray, WT11A-KG. R. Signer, WT 6A-KP.R. Simmons, POB 2B-SQNB. N. Smith (EDMS), MPB 1E-M


May 10, 2012

Ms. Dana Waits

State of Tennessee




Dear Ms. Waits:

SEQUOYAH NUCLEAR PLANT - DISCHARGE MONITORING REPORT FOR APRIL 2012

Enclosed is the April 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. If you have anyquestions or need additional information, please contact Brad Love at (423) 843-6714 of Sequoyah'sEnvironmental staff.



President


Enclosures






PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different)

Name _ _™A ^EQ^YA^N^CLE^R^l^NTAddress, _P.O.BOX2goo. I

(LNTEROFRCEOPS^N^QN)SODDX-DAISY^N^7384

Facjljty_ jn^-^QUOY^NUCLEAR PLANJLocalon_ JH/WIITpILQQyMY

ATTN: Brad Love


From


MONITORING PERIOD

I DAYYEAR MO DAY

To

YEAR MO

12 04 01 12 04 30

MAJOR

(SUBR01)

F - FINAL

DIFFUSER DISCHARGE

EFFLUENT

*** NO DISCHARGE [^] ***NOTE: Read instructions before completing thisform



00010 1 0

EFFLUENT GROSS


00010 Z 0

INSTREAM MONITORING


UPSTRM DEG.C

00016 1 S

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS


50060 1 0

EFFLUENT GROSS


82234 1 0

EFFLUENT GROSS

SAMPLEMEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLEMEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

&*kA4>AA4r4t

MAXIMUM

********

********

********

********

1718

Req. Mon.DAILY MAX

********

DAILY MX

UNITS

03

MGD

62

DEG

C/HR

MINIMUM

********

********

********

********

AVERAGE

0.022

0.1

MOAVG********

MAXIMUM

33.8

Req. Mon.DAILY MAX

22.2

30.5

DAILY MX

DAILY MX

0.059

0.1

DAILY MAX

UNITS

04

DEG.C.

04

DEG.C.

04

DEG.C.

19

MG/L


NO.

EXFREQUENCY

OF

ANALYSIS

30/30

CONTI

NUOUS

30/30

CONTI

NUOUS

30/30

CONTI

NUOUS

30/30

CONTI

NUOUS

26/30

FIVE PER

WEEK

30/30

CONTI

NUOUS

SAMPLE

TYPE

RCORDR

CALCTD

MODELD

CALCTD

CALCTD

CALCTD

RCORDR

RCORDR

GRAB

CALCTD

CALCTD

CALCTD

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certifyunder penalty of law that this document and all attachments were prepared under mydirectionor supervision in accordance witha system designed to assure that qualifiedpersoniproperly gatherand evaluatethe information submitted. Basedon myinquiry ofthe personor!persons who manage thesystem, orthosepersons directly responsible forgathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. I am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTS ANDEXPLANATION OF ANYVIOLATIONS (Referenceall attachments here)

No closed mode operation. Veliger monitoring datais included as an attachment. Spectrus CT1300 treatment began on4/30/12 (max. calc. cone, was0.037mg/L~limit 0.050mg/L).


ice President

ature of principal executiveAcer or authorized agent

423 843-7001

AREA

CODENUMBER

12 05

YEAR MO

Page 1 of 1

09

DAY

Sample DateMean # of

ZM/m3% Settlers

Water


Mean# of

Asiatic

Clams/m3

Water

Temp. (°C)LOCATION SUB LOCATION

01/03/2012 14 100 26 01/03/2012 0 26 1-25-545

01/10/2012 0 0 9 01/10/2011 0 9 RCW

01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234

01/24/2012 0 0 13 01/24/2012 0 13 1-25-545

01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545

02/07/2012 0 0 12 02/07/2012 0 12 1-25-545

02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234

02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545

02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234

03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234

03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234

03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234

03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234

04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234

04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234

04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234

NOTES: %

Gravid Asiatic

Clam

COLLECTED BY

PKSWBE

PB

WDT

CR

BB

WE

CR

WBE

WBE

WBE

WBE

WBE

PB

PB

MJW


Name _ _™A ÊQ^^HJ^CÊÎ^^NTjMdress__P.a.BOX2p00_

aNTERgFRCEOPSJN^QN)SOTE^-^1SY1_TN_37384

FacHjty_ JVA;J^QUOY/W NUCLEAR PLANJ

J^(Ôii_Jl>ÎIJPiLCOUJirY^

ATTN: Brad Love


From

TN0026450 101 T


MONITORING PERIOD

YEAR MO DAY

To

YEAR MO DAY

12 04 01 12 04 30

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE [^] ***NOTE: Readinstructions before completing thisform.



CERIODAPHNIA

TRP3B 1 0

EFFLUENT GROSS


PIMEPHALES

TRP6C 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE MAXIMUM UNITS

********

********

MINIMUM

MonitoringNot Required

43.2

MINIMUM

MonitoringNot Required

43.2

MIMINUM

AVERAGE

********

********

********

MAXIMUM UNITS

23

PERCENT

23

PERCENT


NO.

EX

FREQUENCYOF

ANALYSIS

SEMI

ANNUAL

SEMI

ANNUAL

SAMPLE

TYPE

COMPOS

COMPOS

NAMEnTTLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under mydirection or supervision in accordance with a system designed to assure that qualified personiproperly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. Iam aware that there are significant penalties for submitting false infonnation,including the possibility of fine and imprisonment for knowing violations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTSAND EXPLANATION OFANY VIOLATIONS (Referenceallattachmentshere)

Toxicity was not sampled in April 2012.


ite Vice President


423 843-7001

AREA

CODENUMBER

12 05

YEAR MO

Page 1 of 1

DAY


Name _ JVA-SEQUO^|^NJ£CLE^RÎ^NTM*ress__P.Oi_BOX_200JL

aNIERQFRCEOPSJItSQN)SODDY.-ÎSYaJN_37384

FacjHty_ JV^-^QUOYÎSKiQLEAR PLANJJuration. JlîypjliÛNJY

ATTN: Brad Love

NATIONAL POLLUTANT DISCHARGE ELIMINATION SYSTEM (NPDES)

DISCHARGE MONITORING REPORT (DMR)

From

TN0026450 103 G


MONITORING PERIOD

YEAR MO DAY

To

YEAR I MO DAY

12 04 01 12 | 04 30

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE

NOTE: Read instructions before completing this form.PARAMETER QUANTITY OR LOADING QUALITY OR CONCENTRATION

PH

00400 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

OIL AND GREASE

00556 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

AVERAGE

4r4r4r4r4r4r4r4r

********

********

1.127

Req. Mon.MOAVG

MAXIMUM

********

********

********

1.268

Req. MonDAILY MX

UNITS

03

MGD

MINIMUM

6

MINIMUM

********

********

********

AVERAGE

********

9

30

MOAVG

<6

15

MOAVG

MAXIMUM

MAXIMUM

10

100

DAILY MX

<6

20

DAILY MX

UNITS

12

SU

19

MG/L

19

MG/L


NO.

EX

FREQUENCYOF

ANALYSIS

15/30

THREE/

WEEK

2/30

TWICE/

MONTH

2/30

TWICE/

MONTH

30/30

SEE

PERMIT

SAMPLE

TYPE

GRAB

GRAB

GRAB

GRAB

GRAB

GRAB

RCORDR

RCORDR

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER I Certify under penalty of law that this document and all attachments were prepared under my

Al^yafrSiteVice President

TELEPHONE DATE

John T. Carlin


properly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. 1am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.

423 843-7001 12 05 09SIGNAL[URE OF PRINCIPAL EXECUTIVE

CER OR AUTHORIZED AGENTTYPED OR PRINTED

AREA


COMMENTS ANDEXPLANATION OF ANYVIOLATIONS (Referenceall attachmentshere)

EPA Form 3320-1 (REV 3/99) Previous editions may be used Page 1 of 1


Name _ J™A ^EQ^^^f^C^^RJPLANT^9^SS__£.OJtoX_200g_

(LNraTOFRCEOP^r*£<^SOTC^-^1SYJ1N_37384

Facj|ity__ _TM_-SEQUOYJW NUCLEAR PLANJMcatjprL. Jd>^iypjLQQUNJY

ATTN: Brad Love


TN0026450 110 G


From

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE [xx]



To

YEAR MO DAY

12 04 01 12 04 30NOTE: Read Instructions before completing this form.

PARAMETER


00010 1 0



00010 Z 0

INSTREAM MONITORING


UPSTRM DEG.C

00016 1 0



50050 1 0



50060 1 0



82234 1 0


SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

QUANTITY OR LOADING

AVERAGE

********

********

********

********

********

MAXIMUM

********

********

********

Req. Mon.DAILY MX

********

2

DAILY MX

UNITS

03

MGD

04

DEGC

MINIMUM

********

********

********

********

********

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certifyunder penaltyof lawthat this document and allattachmentswere preparedunder mydirectionor supervision in accordance with a system designed to assure that qualified personnelproperlygather and evaluate the informationsubmitted. Based on my inquiryof the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the informationsubmitted is, to the best of my knowledge and belief, true, accurate,and complete. I am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.

John T. Carlin


AVERAGE

********

********

********

********

0.1

MOAVG********

********

MAXIMUM

REPORT

DAILY MX

30.5

DAILY MX

DAILY MX

********

0.1

DAILY MX

UNITS

04

DEGC

04

DEGC

04

DEGC

19

MG/L

NO.

EX

TELEPHONE

FREQUENCYOF

ANALYSIS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

Five per

Week

CONTIN

UOUS

SAMPLE

TYPE

CALCTD

CALCTD

CALCTD

RCORDR

CALCTD

CALCTD

DATE

423 843-7001 12Sequoyah Site Vice President

TYPED OR PRINTED


CODENUMBER

05 09

YEAR MO DAY

COMMENTS ANDEXPLANATION OFANYVIOLATIONS (Reference allattachments here)No Discharge this Period



Name _ JTVA ^M^^HJ^C^ÎÎ^NTMdre^Ji.OJ30XjragO_

(LNTCROFRCEOPS^N^QN)SODr^-^|SYJlN_37384

Facjjjty_ J^^-^QUOY^NUCLEAR PLANJj^(ôiL_Ji>MiUPJ^QQUr£nL-

ATTN: Brad Love


From

TN0026450 110 T


MONITORING PERIOD

YEAR MO DAY

To

YEAR MO DAY

12 04 01 12 04 30

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE [xx] ***NOTE: Read instructions before completing,this form



EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPE


CERIODAPHNIA

TRP3B 1 0 0



PIMEPHALES

TRP6C 1 0 0


SAMPLEMEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

MAXIMUM

********

********

UNITS MINIMUM

43.2

MINIMUM

43.2

MINIMUM

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certifyunder penalty of lawthat this document and all attachments were prepared under mydirection or supervision in accordance with a system designed to assure that qualified personnelproperly gather and evaluate the informationsubmitted. Based on my inquiryof the person orpersons who manage the system, or those persons directly responsible for gathering theinfonnation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. I am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowingviolations.

John T. Carlln


TYPED OR PRINTED

COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here)No Discharge this Period


SIGNATUBtfOF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

AVERAGE MAXIMUM

********

********

********

ite Vice President

UNITS

23

PERCENT

23

PERCENT

TELEPHONE

423 843-7001

AREA

CODENUMBER

SEMI

ANNUAL

COMPOS

SEMI

ANNUAL

COMPOS

DATE

12 05 09

YEAR MO DAY

Page 1 of 1


Name __™A ^raUO^^r^CL^R_PLANT^p^ss_JP.O^BOX2p0g_

aNJEROFFl(^OPS^N-SQN)SQD[^-DAISYJ^7^4

Fac|Hty_ J]v^-^QyOY>^lsmQLEAR PLANJjx(^ojLJl>MiUPJLQQyiirY

ATTN: Brad Love


From

TN0026450 118 G


MONITORING PERIOD

YEAR MO DAY

To

YEAR MO DAY

12 04 01 12 04 30

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE [xx] ***NOTE: Read instructions before completing this form



00300 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

SOLIDS, SETTLEABLE

00545 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLEMEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

Req, Mon.MOAVG

MAXIMUM

********

********

AtirftAAAfAfA

********

Req. Mon.DAILY MX

UNITS

03

MGD

MINIMUM

2

MINIMUM

**♦***♦♦

AVERAGE

********

********

MAXIMUM

********.

100

DAILY MX

DAILY MX

********

UNITS

19

MG/L

19

MG/L

25

ML/L


NO.

EX

FREQUENCYOF

ANALYSIS

TWICE/

WEEK

TWICE/

WEEK

ONCE/

MONTH

ONCE/

BATCH

SAMPLE

TYPE

GRAB

GRAB

GRAB

ESTIMA

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty of law that this document and all attachments were prepared under mydirectionor supervision in accordance witha system designed to assure that qualifiedpersonnelproperly gather and evaluate the infonnation submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. I am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTSAND EXPLANATION OF ANYVIOLATIONS (Reference all attachmentshere)

During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.


Site Vice President


423 843-7001

AREA

CODENUMBER

12 05

YEAR MO

Page 1 of 1

09

DAY


DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT- April 2012 DMR


DOCUMENT PREPARED BY: Brad Love

DATE: 5/4/2012

CONCURRENCES

Name R

V

C

N


B.M. Love X tf^^- sN1^L.M. Koby X "Sl'S/lz-J.A. Cross X MUM>(A/tUp* ' <rhl> >-





June 12, 2012

Ms. Christina MorganTennessee Department of Environment

and Conservation

Division of Water Pollution Control



Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) -NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FORMAY 2012

Enclosed is the May 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. OnMay 24, 2012 at 15:27 Eastern Daylight Time (EDT) visible oil sheen was identified on theeast side of the essential raw cooling water (ERCW) intake building at SQN on theTennessee River.

The source of the oil sheen was caused by an oil drip pan overflow located at the C-BERCW traveling water screen gear drive. The leak was immediately secured and thedischarge was stopped. The estimated quantity of oil discharged is less than one gallon.Because the oil spill occurred in a low flow area and favorable meteorological conditions(light winds, no rain), the water was fairly stagnant and the sheen was easily contained andcleaned up utilizing absorbent booms and pads. TVA provided oral notification to AngelaYoung of the Chattanooga Environmental Field Office via voice message on May 24, 2012.Documentation of the incident and corrective actions are being performed in accordancewith the site's Corrective Action Program.

In a letter to Mr. Vojin Janjic dated December 21, 2011 SQN notified TDEC of upcomingactivities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle18 refueling outage scheduled to begin in October 2012. This project is complete andresulted in the discharge of approximately 700 gallons of water through the SQN low volumewaste treatment process. The results for the pH and TSS collected during discharge havebeen incorporated into the DMR. The results for the TRC have been included as anattachment to the Outfall 103 reporting form.

If you have any questions or need additional information, please contact Brad Love by emailat [email protected] or by phone at (423) 843-6714.

Ms. Christina MorganPage 2June 12, 2012

/ certify under penalty of law that this document and all attachments were prepared undermy direction or supervision in accordance with a system designed to assure that qualifiedpersonnel properly gather and evaluate the information submitted. Based on my inquiryofthe person or persons who manage the system, or those persons directly responsible forgathering the information, the information submitted is, to the best of my knowledge andbelief, true, accurate, and complete. I am aware that there are significant penalties forsubmitting false information, including the possibility of fine and imprisonment for knowingviolations.

Sincerely,

Site

Sequoyahident

uclear Plant

Enclosures




S.D.Booker, MOB 1F-WBNB. E. Brickhouse, BR 4A-CJ. T. Carlin, OPS 4A-SQNJ. A. Cross, POB 2A-SQNT.R. Markum, BR4A-CD. B. Nida, BR 4A-C



J.W. Proffitt, OPS 4C-SQNA. A. Ray,WT11A-KG. R. Signer, WT 6A-KP.R. Simmons, POB 2B-SQNB. N. Smith (EDMS), MPB 1E-M

Valley Authority, Post Office Box 2000, Soddy Daisy, Tennessee 37384-2000

June 12, 2012


and Conservation




Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) -NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FORMAY 2012

Enclosed is the May 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant. OnMay 24, 2012 at 15:27 Eastern Daylight Time (EDT) visible oil sheen was identified on theeast side of the essential raw cooling water (ERCW) intake building at SQN on theTennessee River.

The source of the oil sheen was caused by an oil drip pan overflow located at the C-BERCW traveling water screen gear drive. The leak was immediately secured and thedischarge was stopped. The estimated quantity of oil discharged is less than one gallon.Because the oil spill occurred in a low flow area and favorable meteorological conditions(light winds, no rain), the water was fairly stagnant and the sheen was easily contained andcleaned up utilizing absorbent booms and pads. TVA provided oral notification to AngelaYoung of the Chattanooga Environmental Field Office via voice message on May 24, 2012.Documentation of the incident and corrective actions are being performed in accordancewith the site's Corrective Action Program.

In a letter to Mr. Vojin Janjic dated December 21, 2011 SQN notified TDEC of upcomingactivities for the installation of pilings into bedrock to support a crane during the Unit 2 Cycle18 refueling outage scheduled to begin in October 2012. This project is complete andresulted in the discharge of approximately 700 gallons of water through the SQN low volumewaste treatment process. The results for the pH and TSS collected during discharge havebeen incorporated into the DMR. The results for the TRC have been included as anattachment to the Outfall 103 reporting form.


Ms. Christina MorganPage 2June 12, 2012

/ certifyunderpenalty of law that this document and all attachments were prepared undermy direction or supervision in accordance with a system designed to assure that qualifiedpersonnel properly gather and evaluate the information submitted. Based on my inquiry ofthe person or persons who manage the system, or those persons directly responsible forgathering the information, the information submitted is, to the best of my knowledge andbelief, true, accurate, and complete. I am aware that there are significantpenalties forsubmitting false information, including the possibility of fine and imprisonment forknowingviolations.

lin

be PresidentSequoyah Nuclear Plant

Enclosures

cc (Enclosures):Chattanooga Environmental Field Office U.S. Nuclear Regulatory CommissionDivision of Water Pollution Control Attn: Document Control Desk

State Office Building, Suite 550 Washington, DC 20555540 McCallie Avenue


PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name _ _TVA ^M^^HL^C^^RJPI^NT^dress__P.OLBOX2pop_

aNJEEROFRCEOPS^N^QN)SOTD^-JDAlSY1jrN_J7384

FacHity__ _TVA_- SEQUOY^NUCLEAR PLANJLocation. Jl^lUPiLQQUNJY

ATTN: Brad Love


From



ToYEAR MO DAY

12 05 01 12 05 31

MAJOR

(SUBR01)

F - FINAL

DIFFUSER DISCHARGE

EFFLUENT

*** NO DISCHARGE Q ***NOTE: Read Instructions before completing this form



00010 1 0

EFFLUENT GROSS


00010 Z 0

INSTREAM MONITORING


UPSTRM DEG.C

00016 1 S

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS


50060 1 0

EFFLUENT GROSS


82234 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

ftttftftftftftft

*#*♦*###

ftftftftft&Aft

MAXIMUM

********

********

********

1719

Req. Mon.DAILY MAX

wwwwAft&Af

DAILY MX

UNITS

03

MGD

62

DEG

C/HR

MINIMUM

********

********

********

AVERAGE

********

********

0.019

0.1

MOAVG********

MAXIMUM

39.4

Req. Mon.DAILY MAX

28.3

30.5

DAILY MX

1

DAILY MX

********

0.034

0.1

DAILY MAX

UNITS

04

DEG.C.

04

DEG.C.

04

DEG.C.

19

MG/L

Form Approved.

OMB No. 2040-0004

NO.

EX

FREQUENCYOF

ANALYSIS

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

31/31

CONTI

NUOUS

18/31

FIVE PER

WEEK

31/31

CONTI

NUOUS

SAMPLE

TYPE

RCORDR

CALCTD

MODELD

CALCTD

CALCTD

CALCTD

RCORDR

RCORDR

GRAB

CALCTD

CALCTD

CALCTD

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify underpenaltyof lawthat this documentand allattachments werepreparedundermydirection or supervision inaccordancewith a systemdesignedto assure that qualified personnelproperly gatherand evaluatethe information submitted. Basedon myinquiry ofthe personorpersons whomanagethe system,or those persons directly responsible forgathering theinformation, the information submitted is, to the bestofmyknowledge and belief, true,accurate,and complete. Iam awarethat there are significant penaltiesforsubmitting false information,includingthe possibility offine and imprisonmentfor knowing violations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here)No closed mode operation. Veliger monitoring data is included as an attachment. B/CTP chemical injections during this period included 1. Floguard MS6236 (max calc cone was 0032mg/L~limit0.2mg/L) 2. Biodetergent 73551 (max. calc. cone, was 0.032mg/L-limit 2.0mg/L) 3. Spectrus CT1300 (max. calc. cone, was 0.041 mg/L~limit 0.050mg/L).EPAForm 3320-1 (REV3/99) Previous editions may be used ~—

: OF PRINCIPAL EXECUTIVEFICER OR AUTHORIZED AGENT

423 843-7001

AREA

CODENUMBER

12 06

YEAR MO

Page 1 of 1

12

DAY

Sample DateMean# of

ZM/m3% Settlers

Water


Mean# of

Asiatic

Clams/m3

Water

Temp. (°C)

NOTES: %

LOCATION SUB LOCATION Gravid Asiatic

Clam

COLLECTED BY

01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE

02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE

03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE

03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE

03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE

03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJWMay 2012 No Samples Collected

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocation if Different)

Name __ _™A ^M^^HJ^^^F^l^NT^(fes^jp.o^BOXigog.

O.NIERQFjFlCEOPSJ.N^QN)SODDX-DAISYJ1N_37384

FacjHty__ _TV^-SEQUOYM IWO^^ PLANJJ^c^on_Jl>MiUPiLQQyiOY

ATTN: Brad Love


From

TN0026450 101 T


YEAR

MONITORING PERIOD

MO DAY

To

YEAR MO DAY

12 05 01 12 05 31

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE Q ***NOTE: Read instructions before completing this form.



CERIODAPHNIA

TRP3B 1 0

EFFLUENT GROSS


PIMEPHALES

TRP6C 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE MAXIMUM

♦♦♦♦♦♦♦♦

wxkwAAiMIe wt&w&&1iitwt

UNITS MINIMUM

>100.0

43.2

MINIMUM

>100.0

43.2

MIM1NUM

AVERAGE MAXIMUM

♦♦&4r4r A4f4r

UNITS

23

PERCENT

23

PERCENT

Form Approved.

OMB No. 2040-0004

NO.

EX

FREQUENCYOF

ANALYSIS

1/180

SEMI

ANNUAL

1/180

SEMI

ANNUAL

SAMPLE

TYPE

COMPOS

COMPOS

COMPOS

COMPOS

NAMEHTTLE PRINCIPAL EXECUTIVE OFFICER ICertify under penallyof lawthat this documentand allattachmentswere preparedundermydirection or supervisioninaccordancewitha system designed to assure that qualified person™properly gatherand evaluatethe information submitted. Basedon my inquiry of the personorpersonswho managethe system, orthose personsdirectly responsible forgathering theinformation, the information submitted is, to the best of my knowledge and belief,true,accurate,andcomplete. Iam awarethatthereare significant penalties forsubmitting false information,includingthe possibilityof fine and imprisonmentforknowingviolations.

TELEPHONE DATE

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference allattachments here)Toxicity sampling was performed May 6-11. The report is included as an attachment.


Vice President

E OF PRINCIPAL EXECUTIVEOR AUTHORIZED AGENT

423 843-7001

AREA

CODENUMBER

12 06

YEAR MO

Page 1 of 1

12

DAY



MAJOR

(SUBR01)

F - FINAL


EFFLUENT

Form Approved.

OMB No. 2040-0004


Name __ _™A ^EQI^^^NJK^E^R^LANT^(fess__P.a_BOX2000.

aNTERQFFICEOPSir^SQN)SODDY.-DAlSYJLTN_J7384

Facjlity_ jn^-^QUOY^NUCLEAR PLANJUcatjpn_ Jd/MiyPILQQLINJY

ATTN: Brad Love

PARAMETER

From


ToYEAR MO DAY

12 05 01 12 05 31NO DISCHARGE \_] ***


PH

00400 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

OIL AND GREASE

00556 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

QUANTITY OR LOADING

AVERAGE

1.038

Req. Mon.MOAVG

MAXIMUM

********

********

********

1.216

Req. MonDAILY MX

UNITS

03

MGD

MINIMUM

6

MINIMUM

********

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certifyunder penaltyof lawthat this documentand allattachmentswere preparedundermydirection or supervisionin accordancewitha system designed to assure that qualifiedpersonnelproperly gather and evaluate the informationsubmitted. Based on my inquiryof the person orpersons who manage the system, or those persons directlyresponsible for gathering theinformation, the Information submitted is, to the best of my knowledge and belief, true, accurate,and complete. Iam aware that there are significantpenalties forsubmittingfalse information,including the possibility of fine and imprisonment for knowing violations.

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here)Outfall 103 TRC analysis collected during discharge of Steam Generator Process Water from drilling core pilings is included as an attachment.



AVERAGE

********

30

MOAVG<5

15

MOAVGWW WW ft ft "$1it

Aft&ft&ftftft

MAXIMUM

MAXIMUM

9

100

DAILY MX

<6

20

DAILY MX********

********

UNITS

12

SU

19

MG/L

19

MG/L

NO.

EX

FREQUENCYOF

ANALYSIS

15/31

THREE/

WEEK

2/31

TWICE/

MONTH

2/31

TWICE/

MONTH

31/31

SEE

PERMIT

Page 1 of 1

SAMPLE

TYPE

GRAB

GRAB

GRAB

GRAB

GRAB

GRAB

RCORDR

RCORDR

DMR Attachment

Outfall 103: TRC Sample collected during discharge of Steam Generator Projectdischarge of process water from drilling core pilings. Reference DMR Cover Letter.

Outfall 103

Date/Time Collected TRC Analysis Date/Time Analyst

5/15/2012/11:10 0.03 mg/L 5/15/2012/11:24 MLW


Name ___™A-SEQI^^^r^CL^I^^NTMdress__P.a_BOX2pog.

aNJEROFRCEOPSlN^QN)SODE^-DAISYJW^7384

FacjHty___ JYA- SEQUOY^NyCLEAR PLANJLocatLQD_ Jl/M'UPILCQLINJY

ATTN: Brad Love


MAJOR

(SUBR01)

F - FINAL


EFFLUENT

Form Approved.

OMB No. 2040-0004

From

TN0026450 110 G


MONITORING PERIODY^AR MO DAY

To

YEAR MO DAY

12 05 01 12 05 31NO DISCHARGE

NOTE: Read Instructions before completing this form.

XX


EX


00010 1 0



00010 Z 0

INSTREAM MONITORING


UPSTRM DEG.C

00016 1 0



50050 1 0



50060 1 0



82234 1 0


SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

AVERAGE

********

MAXIMUM

WWIXIXXW

Req. Mon.DAILY MX

DAILY MX

UNITS

03

MGD

04

DEGC

MINIMUM

******£*

********

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER Certify under penaltyof lawthat this documentand allattachmentswere prepared under mydirection or supervisioninaccordance witha systemdesigned to assure that qualified personnelproperlygather and evaluate the Information submitted.Based on myinquiryof the person orpersons whomanage the system,or those persons directly responsibleforgatheringtheinformation, the information submitted is, to the best of myknowledgeand belief, true, accurate,and complete. Iam aware that there are significantpenalties for submittingfalse information,includingthe possibilityof fine and imprisonmentfor knowing violations.

John T. Carlin

AVERAGE

********

********

********

0.1

MOAVG

MAXIMUM

REPORT

DAILY MX

30.5

DAILY MX

5

DAILY MX

0.1

DAILY MX

********

UNITS

04

DEGC

04

DEGC

04

DEGC

19

MG/L

TELEPHONE

FREQUENCYOF

ANALYSIS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

Five per

Week

CONTIN

UOUS

SAMPLE

TYPE

CALCTD

CALCTD

CALCTD

RCORDR

CALCTD

CALCTD

DATE

Sequoyah Site Vice President 423 843-7001 12 06 12

TYPED OR PRINTED



SIGNATUREOFFK

PRINCIPAL EXECUTIVE

>R AUTHORIZED AGENT AREA

CODENUMBER YEAR MO

Page 1 of 1

DAY

PERMITTEE NAME/ADDRESS (Include Facility NameA.ocaVon if Different)

Name __ _™A ^TO^^^r^CL^R^LANT^d^ss____P.a.BOX2p00.

aNTERQFRCEOPMri^QN)S0DDX-J^1SY1JN_37384

Facjljty__ JFW.- SEQUOY/^NUCLEAR PLANJLgcatJon_Jiy^

ATTN: Brad Love


From

TN0026450 110 T



To

YEAR MO DAY

12 05 01 12 05 31

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE XX

Form Approved.

OMB No. 2040-0004

NOTE: Read Instructions before completing this form.

PARAMETER

x-QUANTITY OR LOADING QUALITY OR CONCENTRATION NO.

EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPE

AVERAGE MAXIMUM • UNITS MINIMUM AVERAGE MAXIMUM UNITS


CERIODAPHNIA

TRP3B 1 0 0


SAMPLE

MEASUREMENT

******** w AAwA ft ff§t**

****

******** ********

23


******** ftftfttttt'Wt'lt43.2

MINIMUM

******** ******** SEMI

ANNUAL

COMPOS


PIMEPHALES

TRP6C 1 0 0


SAMPLE

MEASUREMENT

**********

******** ********

23

PERCENTPERMIT

REQUIREMENT

**************** 43.2

MINIMUM

******** ******** SEMI

ANNUAL

COMPOS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

NAMEATITLE PRINCIPAL EXECUTIVE OFFICER

John T. Carlin


TYPED OR PRINTED

I Certifyunder penalty of law that this document and all attachments were prepared under mydirection or supervision in accordance with a system designed to assure that qualified personnelproperly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. I am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.





TELEPHONE

423 843-7001

AREA

CODENUMBER

DATE

12 06

YEAR MO

Page 1 of 1

12

DAY


Name __™*i?EQWW^IWCLEARPLANTMdress_ J^OJJQX20go.

aNJlEOFRCEOPSlN^QN)SOTDY_-DA1SYJ:N_37384

Facjnty__ JA^-SEQUOYM MOLEAR PLANJJ^catjpn_ ji>Miynpj^coyNTY .

ATTN: Brad Love


From

TN0026450 118 G



To

YEAR MO DAY

12 05 01 12 05 31

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE [xx] ***NOTE: Read Instructions before completing this form

Form Approved.

OMB No. 2040-0004


EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPEAVERAGE MAXIMUM UNITS MINIMUM


00300 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT2

MINIMUMSOLIDS, TOTAL SUSPENDED

00530 1 0

EFFLUENT GROSS

SOLIDS, SETTLEABLE

00545 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

********

********

Req. Mon.MOAVG

********

********

Req. Mon.DAILY MX

03

MGD

NAMEHTTLE PRINCIPAL EXECUTIVE OFFICER ICertifyunder penaltyof lawthat this document and allattachments were preparedunder mydirectionor supervision in accordance witha system designed to assure that qualifiedpersonnelproperlygather and evaluate the information submitted. Based on my inquiryof the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the informationsubmitted is, to the best of my knowledge and belief, true, accurate,and complete. Iam aware that there are significant penalties for submitting false information,includingthe possibilityof fine and imprisonment for knowing violations.

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here)During this reporting period, there has been no flow from the Dredge Pond other than that resulting from rainfall.


SIGNATI

OFFICEi

AVERAGE

********

********

MAXIMUM

********

100

DAILY MX

DAILY MX

resident

PRINCIPAL EXECUTIVE

AUTHORIZED AGENT

UNITS

19

MG/L

19

MG/L

25

ML/L

TELEPHONE

423 843-7001

AREA

CODENUMBER

TWICE/

WEEK

TWICE/

WEEK

ONCE/

MONTH

ONCE/

BATCH

GRAB

GRAB

GRAB

ESTIMA

DATE

12 06 12

YEAR MO DAY

Page 1 of 1

TENNESSEE VALLEY AUTHORITY TOXICITY TEST REPORT

INTRODUCTION / EXECUTIVE SUMMARY

Report Date: June 11, 2012 1. Facility / Discharger: Sequoyah Nuclear Plant / TVA 2. County / State: Hamilton / Tennessee 3. NPDES Permit #: TN0026450 4. Type of Facility: Nuclear-Fueled Electric Generating Plant 5. Design Flow (MGD): 1,579 6. Receiving Stream: Tennessee River (TRM 483.6) 7. 1Q10: 3,491 8. Outfall Tested: 101 9. Dates Sampled: May 06 – 11, 2012 10. Average Flow on Days Sampled (MGD): 1715.29, 1713.71, 1700.72 11. Pertinent Site Conditions: Production / operation data will be provided upon request.

12. Test Dates: May 08 – 15, 2012 13. Test Type: Short-term Chronic Definitive 14. Test Species: Fathead Minnows (Pimephales promelas) Daphnids (Ceriodaphnia dubia) 15. Concentrations Tested (%): Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 Intake: 100

Pimephales promelas: UV treated Outfall 101: 10.8, 21.6, 43.2, 86.4, 100 UV treated Intake: 100

16. Permit Limit Endpoint (%): Outfall 101: IC25 = 43.2% 17. Test Results: Outfall 101: Pimephales promelas: IC25 > 100% Ceriodaphnia dubia: IC25 > 100%

UV treated Outfall 101: Pimephales promelas: IC25 > 100%

Page 1 of 110

18. Facility Contact: Brad Love Phone #: (423) 843-6714 19. Consulting / Testing Lab: Environmental Testing Solutions, Inc. 20. Lab Contact: Jim Sumner Phone #: (828) 350-9364 21. TVA Contact: Donald W. Snodgrass Phone #: (256) 386-2787 22. Notes: Outfall 101 samples collected May 06 – 11, 2012, showed no toxic effects to

fathead minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 2 of 110

METHODS SUMMARY Samples: 1. Sampling Point: Outfall 101, Intake 2. Sample Type: Composite 3. Sample Information:

Sample ID

Date (MM-DD-YY)

Time (ET) Collected

Date (MM-DD-YY)

Time (ET) Received

Arrival Temp. (°C)

Initial TRC* (mg/L)

Date (MM-DD-YY)

Time (ET) Last Used By

101 05-06-12 0855 to 05-07-12 0755 05-07-12 1429 1.8, 1.8† <0.10 05-08-12 1130

05-09-12 1030

Intake 05-06-12 0910 to 05-07-12 0810 05-07-12 1429 1.0 <0.10 05-08-12 1130

05-09-12 1030

101 05-08-12 0655 to 05-09-12 0555 05-09-12 1300 1.6, 1.7† <0.10 05-10-12 1053

05-11-12 1030

Intake 05-08-12 0710 to 05-09-12 0610 05-09-12 1300 1.6 <0.10 05-10-12 1053

05-11-12 1030

101 05-10-12 0655 to 05-11-12 0555 05-11-12 1225 0.5, 0.6† <0.10

05-12-12 1030 05-13-12 1032 05-14-12 1030

Intake 05-10-12 0710 to 05-11-12 0610 05-11-12 1225 2.1 <0.10

05-12-12 1030 05-13-12 1032 05-14-12 1030

*TRC = Total Residual Chlorine †Samples were collected in two 2.5 gallon cubitainers. Temperature was measured in each cubitainer upon arrival.

4. Sample Manipulation: Samples from Outfall 101 and intake were warmed to test temperature

(25.0 ± 1.0oC) in a warm water bath.

Aliquots of Outfall 101 and Intake samples were UV-treated through a 40-watt Smart® UV Sterilizer (manufactured by Emperor Aquatics, Inc.) for 2 minutes.

Page 3 of 110

Pimephales promelas Ceriodaphnia dubia Test Organisms: 1. Source: Aquatox, Inc. In-house Cultures 2. Age: 19.2 – 19.5 hours old < 24-hours old Test Method Summary: 1. Test Conditions: Static, Renewal Static, Renewal 2. Test Duration: 7 days Until at least 60% of control

females have 3 broods 3. Control / Dilution Water: Moderately Hard Synthetic Moderately Hard Synthetic 4. Number of Replicates: 4 10 5. Organisms per Replicate: 10 1 6. Test Initiation: (Date/Time) Outfall 101 05-08-12 1130 ET 05-08-12 0935 ET UV Treated Outfall 101 05-08-12 1112 ET

7. Test Termination: (Date/Time) Outfall 101 05-15-12 1030 ET 05-15-12 0842 ET UV Treated Outfall 101 05-15-12 1015 ET

8. Test Temperature: Outfall 101: Mean = 24.8oC Mean = 25.0oC (24.5 – 25.1oC) (24.7 – 25.3oC)

Test Temperature: UV-Treated Outfall 101: Mean = 24.8oC (24.3 – 25.2oC)

9. Physical / Chemical Measurements: Alkalinity, hardness, total residual chlorine, and conductivity were

measured at the laboratory in each 100% sample. Daily temperatures were measured in one replicate for each test concentration. Pre- and post-exposure test solutions were analyzed daily for pH and dissolved oxygen.

10. Statistics: Statistics were performed according to methods prescribed by EPA

using ToxCalc version 5.0 statistical software (Tidepool Scientific Software, McKinneyville, CA).

Page 4 of 110

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 1. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration) Conducted May 08 – 15, 2012 using effluent from Outfall 101.

Test Solutions

(% Effluent)

Percent Surviving (time interval used – days)

1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 98 98 98 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100

Test Solutions (% Effluent)

Mean Dry Weight (mg) (replicate number)

1 2 3 4 Mean Control 0.658 0.686 0.707 0.778 0.707 10.8% 0.624 0.648 0.699 0.647 0.655 21.6% 0.629 0.717 0.594 0.716 0.664 43.2% 0.678 0.674 0.642 0.674 0.667 86.4% 0.660 0.604 0.657 0.648 0.642 100.0% 0.621 0.634 0.586 0.682 0.631 Intake 0.734 0.634 0.693 0.716 0.694

IC25 Value: > 100% Permit Limit: 43.2% 95% Confidence Limits:

Upper Limit: NA Lower Limit: NA

Calculated TU Estimates: < 1.0 TUc* Permit Limit: 2.3 TUc

*TUa = 100/LC50: TUc = 100/ IC25

Page 5 of 110

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration)

Conducted May 08 – 15, 2012 using effluent from Outfall 101.

Test

Solutions (% Effluent)


1 2 3 4 5 6 7

Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100


Reproduction (#young/female/7 days) Data (replicate number)

1 2 3 4 5 6 7 8 9 10 Mean

Control 33 32 30 31 36 30 33 34 31 29 31.9 10.8% 33 33 30 33 31 32 28 35 35 32 32.2 21.6% 36 32 36 33 31 32 35 32 35 33 33.5 43.2% 35 31 33 39 33 30 35 37 33 33 33.9 86.4% 34 36 32 35 37 35 34 34 33 35 34.5 100.0% 37 36 35 35 37 38 33 36 35 36 35.8

IC25 Value: > 100% Permit Limit: 43.2% 95% Confidence Limits:


Calculated TU Estimates: < 1.0 TUc* Permit Limit: 2.3 TUc

*TUa = 100/LC50: TUc = 100/ IC25

Page 6 of 110

TOXICITY TEST RESULTS (see Appendix C for Bench Sheets) 2. Results of a Ceriodaphnia dubia Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration)

Conducted May 08 – 15, 2012 using water from Intake

Test

Solutions (% Effluent)


1 2 3 4 5 6 7

Control 100 100 100 100 100 100 100 Intake 100 100 100 100 100 100 100


Reproduction (#young/female/7 days) Data (replicate number)

1 2 3 4 5 6 7 8 9 10 Mean

Control 30 33 33 30 31 29 32 31 31 26 30.6 Intake 33 35 29 32 31 36 32 31 33 31 32.3

IC25 Value: > 100% Permit Limit: N/A 95% Confidence Limits:


Calculated TU Estimates: < 1.0 TUc* Permit Limit: N/A

*TUa = 100/LC50: TUc = 100/ IC25

Page 7 of 110

TOXICITY TEST RESULTS, UV-TREATED (see Appendix C for Bench Sheets) 3. Results of a Pimephales promelas Chronic/ 7-day Toxicity Test. (Genus species) (Type / Duration)

Conducted May 08 – 15, 2012 using effluent from UV Treated Outfall 101.

Test Solutions

(% Effluent)


1 2 3 4 5 6 7 Control 100 100 100 100 100 100 100 10.8% 100 100 100 100 100 100 100 21.6% 100 100 100 100 100 100 100 43.2% 100 100 100 100 100 100 100 86.4% 100 100 100 100 100 100 100 100.0% 100 100 100 100 100 100 100 Intake 100 100 98 98 98 98 98


Mean Dry Weight (mg) (replicate number)

1 2 3 4 Mean Control 0.591 0.647 0.507 0.599 0.586 10.8% 0.646 0.621 0.616 0.698 0.645 21.6% 0.553 0.622 0.659 0.663 0.624 43.2% 0.565 0.609 0.634 0.606 0.604 86.4% 0.537 0.573 0.610 0.560 0.570 100.0% 0.673 0.680 0.676 0.653 0.671 Intake 0.597 0.592 0.561 0.704 0.614

IC25 Value: > 100% 95% Confidence Limits:


Calculated TU Estimates: < 1.0 TUc*

*TUa = 100/LC50: TUc = 100/ IC25

REFERENCE TOXICANT TEST RESULTS (see Appendix A and D) Species Date Time Duration Toxicant Results (IC25) Pimephales promelas May 08 – 15, 2012 1236 7 days KCl 0.77 g/L

Ceriodaphnia dubia May 08 – 15, 2012 0908 7 days NaCl 1.06 g/L

Page 8 of 110

24.8

-24

.924

.6-

25.0

7.4

-7.

86.

4-

8.0

7.85

-8.

067.

49-

7.90

294

-32

762

-63

87-

89

24.8

-25

.024

.5-

24.9

7.6

-8.

06.

4-

7.9

7.89

-8.

107.

49-

7.86

283

-30

6

24.8

-25

.024

.5-

24.9

7.6

-8.

06.

5-

7.9

7.89

-8.

107.

49-

7.86

276

-29

1

24.8

-25

.024

.6-

25.1

7.6

-8.

06.

5-

8.0

7.86

-8.

117.

47-

7.90

253

-26

5

24.8

-25

.024

.6-

25.0

7.6

-8.

06.

5-

8.0

7.81

-8.

127.

46-

7.90

210

-21

8

24.8

-25

.024

.5-

25.0

7.6

-8.

06.

2-

8.0

7.76

-8.

167.

42-

7.92

188

-19

979

-81

81-

81<

0.10

-<

0.10

24.9

-25

.124

.7-

25.1

7.7

-8.

16.

7-

8.0

7.95

-8.

167.

53-

7.93

182

-19

577

-79

79-

81<

0.10

-<

0.10

24.7

-25

.024

.9-

25.2

7.4

-7.

87.

6-

8.0

7.85

-8.

067.

87-

8.02

294

-32

762

-63

87-

89

24.7

-25

.224

.8-

25.3

7.6

-8.

07.

6-

8.0

7.89

-8.

107.

86-

8.03

283

-30

6

24.7

-25

.224

.8-

25.2

7.6

-8.

07.

6-

8.0

7.89

-8.

107.

86-

8.02

276

-29

1

24.8

-25

.224

.7-

25.2

7.6

-8.

07.

6-

8.1

7.86

-8.

117.

85-

8.03

253

-26

5

24.8

-25

.225

.0-

25.2

7.6

-8.

07.

6-

8.0

7.81

-8.

127.

86-

8.07

210

-21

8

24.8

-25

.224

.8-

25.2

7.6

-8.

07.

6-

8.1

7.76

-8.

167.

87-

8.06

188

-19

979

-81

81-

81<

0.10

-<

0.10

24.8

-25

.224

.8-

25.2

7.7

-8.

17.

8-

8.1

7.95

-8.

167.

87-

8.03

182

-19

577

-79

79-

81<

0.10

-<

0.10

Ove

rall

tem

pera

ture

(o C)

Pim

epha

les p

rom

elas

Cer

ioda

phni

a du

bia

25.0

24.7

25.3

Ave

rage

Min

imum

Max

imum

24.8

24.5

25.1

PHYS

ICA

L/C

HEM

ICA

L SU

MM

AR

Y

perfo

rmed

May

08-

15, 2

012.

Test

Sam

ple

IDTe

mpe

ratu

re (o C

)D

isso

lved

Oxy

gen

(mg/

L)pH

(S.U

.)A

lkal

inity

Har

dnes

s

Wat

er C

hem

istry

Mea

n V

alues

and

Ran

ges f

or P

imep

hale

s pro

mel

as a

nd C

erio

daph

nia

dubi

a T

ests,

Non

-trea

ted

Sequ

oyah

Nuc

lear P

lant (

SQN

) Out

fall 1

01

Initi

alFi

nal

Initi

alFi

nal

Initi

alFi

nal

24.9

24.7

7.1

7.8

24.7

7.9

24.7

7.9

(µm

hos/

cm)

310

88

7.98

7.97

293

283

43.2

%24

.924

.7

7.72

7.68

7.2

21.6

%24

.9

24.7

7.71

86.4

%24

.9

637.

96

7.8

7.8

7.6

7.96

7.2

260

Tota

l Res

idua

lC

hlor

ine

(mg/

L)C

ondu

ctan

ce(m

g/L

CaC

O3)

(mg/

L C

aCO

3)

Pimephales promelas

Con

trol

24.8

24.7

10.8

%

7.68

7.2

< 0.

1080

8119

5

7.2

7.95

7.72

213

7.73

7.2

7.94

Inta

ke

7.6

7.8

100.

0%

25.0

10.8

%24

.925

.0

43.2

%

25.0

190

25.0

24.8

7.9

7.3

8.06

7.75

Ceriodaphnia dubia

Con

trol

24.8

25.0

Inta

ke

21.6

%

86.4

%24

.925

.0

24.9

7.8

7.8

7.97

7.95

293

7.95

310

7.96

6388

7880

< 0.

10

24.9

25.0

7.8

7.8

7.98

260

283

7.9

25.0

7.8

7.8

7.96

7.95

7.95

7.8

7.8

7.95

807.

94

7.98

213

100.

0%25

.025

.07.

9

7.98

78

8.00

< 0.

10

< 0.

1019

5

25.0

81

8.06

7.9

7.9

190

80

Page 9 of 110

24.7

-24

.924

.5-

24.8

7.6

-8.

06.

5-

8.0

7.88

-8.

077.

51-

7.91

300

-31

662

-64

87-

89

24.8

-25

.024

.4-

24.9

7.6

-8.

06.

6-

7.9

7.88

-8.

097.

50-

7.85

283

-30

5

24.8

-25

.024

.5-

24.9

7.6

-8.

06.

5-

8.0

7.89

-8.

107.

50-

7.90

269

-29

1

24.8

-25

.124

.5-

24.8

7.6

-8.

06.

5-

7.9

7.86

-8.

117.

50-

7.89

248

-26

6

24.8

-25

.124

.6-

24.9

7.5

-8.

06.

5-

7.9

7.79

-8.

137.

50-

7.90

207

-22

1

24.8

-25

.224

.3-

24.8

7.6

-8.

16.

5-

7.9

7.76

-8.

157.

50-

7.91

189

-20

179

-79

81-

81<

0.10

-<

0.10

24.8

-25

.224

.5-

24.9

7.6

-8.

26.

6-

8.0

7.94

-8.

167.

52-

7.95

184

-19

677

-79

79-

81<

0.10

-<

0.10

Ove

rall

tem

pera

ture

(o C)

Pim

epha

les p

rom

elas

Inta

ke

100.

0%25

.0

192

25.0

24.7

8.0

24.6

7.9

7.74

216

7.74

7.2

7880

7981

< 0.

10

260

7.71

7.71

7.2

8.02

7.77

198

7.2

7.94

7.94

< 0.

10

Tota

l Res

idua

lC

hlor

ine

(mg/

L)C

ondu

ctan

ce(m

g/L

CaC

O3)

(mg/

L C

aCO

3)63

(µm

hos/

cm)

307

887.

9

7.96

7.2

Pimephales promelas

Con

trol

24.8

24.7

10.8

%

86.4

%24

.9

43.2

%24

.924

.6

7.72

7.70

7.3

21.6

%24

.9

24.6

7.97

7.97

7.97

296

283

24.9

24.7

7.2

7.9

24.7

7.9

7.2

7.9

7.9

Initi

alFi

nal

Initi

alFi

nal

Initi

alFi

nal

PHYS

ICA

L/C

HEM

ICA

L SU

MM

AR

Y

perfo

rmed

May

08-

15, 2

012.

Test

Sam

ple

IDTe

mpe

ratu

re (o C

)D

isso

lved

Oxy

gen

(mg/

L)pH

(S.U

.)A

lkal

inity

Har

dnes

s

Wat

er C

hem

istry

Mea

n V

alues

and

Ran

ges f

or P

imep

hale

s pro

mel

as T

est,

UV

-trea

ted

Sequ

oyah

Nuc

lear P

lant (

SQN

) Out

fall 1

01

Ave

rage

Min

imum

Max

imum

24.8

24.3

25.2

Page 10 of 110

SUMMARY / CONCLUSIONS Outfall 101 samples collected May 06 – 11, 2012, showed no toxic effects to fathead

minnows or daphnids. The resulting IC25 values, for both species, were > 100 percent. Exposure of minnows and daphnids to intake samples resulted in no significant difference from the controls during this study period.

Fathead minnows were also exposed to UV treated Outfall 101 and intake samples since fish pathogens present in intake water have been the suspected cause of interference (anomalous dose response and high variability among replicates) in previous toxicity testing at Sequoyah. At the time this study was conducted, insignificant mortality occurred in minnows exposed to non-treated and UV treated samples.

Page 11 of 110

Appendix A

ADDITIONAL TOXICITY TEST INFORMATION

SUMMARY OF METHODS 1. Pimephales promelas

Tests were conducted according to EPA-821-R-02-013 (October 2002) using four replicates, each containing ten test organisms, per treatment. Test vessels consisted of 500-mL plastic disposable cups, each containing 250-mL of test solution.

2. Ceriodaphnia dubia

Tests were conducted according to EPA-821-R-02-013 (October 2002) using ten replicates, each containing one test organism, per treatment. Test vessels consisted of 30-mL polypropylene cups, each containing 15-mL of test solution.

DEVIATIONS / MODIFICATIONS TO TEST PROTOCOL 1. Pimephales promelas

None


None

DEVIATIONS / MODIFICATIONS TO PRETEST CULTURE OR HOLDING OF TEST ORGANISMS 1. Pimephales promelas

None


None

Page 12 of 110

PHYSICAL AND CHEMICAL METHODS 1. Reagents, Titrants, Buffers, etc.: All chemicals were certified products used before expiration

dates (where applicable).

2. Instruments: All identification, service, and calibration information pertaining to laboratory instruments is recorded in calibration and maintenance logbooks.

3. Temperature was measured by SM 2550 B.

4. Dissolved oxygen was measured by SM 4500 O G. 5. The pH was measured by SM 4500 H+ B. 6. Conductance was measured by SM 2510 B. 7. Alkalinity was measured by SM 2320 B. 8. Total hardness was measured by SM 2340 C. 9. Total residual chlorine was measured by ORION Electrode Method 97-70.

QUALITY ASSURANCE

Toxicity Test Methods: All phases of the study including, but not limited to, sample collection, handling and storage, glassware preparation, test organism culturing/acquisition and acclimation, test organism handling during test, and maintaining appropriate test conditions were conducted according to the protocol as described in this report and EPA-821-R-02-013. Any known deviations were noted during the study and are reported herein.

REFERENCE TOXICANT TESTS (See Appendix D for control chart information) 1. Test Type: 7-day chronic tests with results expressed as IC25 values in g/L KCl or NaCl. 2. Standard Toxicant: Potassium Chloride (KCl crystalline) for Pimephales promelas. Sodium Chloride (NaCl crystalline) for Ceriodaphnia dubia. 3. Dilution Water Used: Moderately hard synthetic water. 4. Statistics: ToxCalc software Version 5.0 was used for statistical analyses.

Page 13 of 110

REFERENCES 1. NPDES Permit No. TN0026450. 2. USEPA. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and

Receiving Waters to Freshwater Organisms, EPA-821-R-02-013 (October 2002). 3. Standard Methods for the Examination of Water and Wastewater, 21st Edition, 2005. 4. Quality Assurance Program: Standard Operating Procedures, Environmental Testing Solutions, Inc (most current version).

Page 14 of 110

Sequoyah Nuclear Plant Biomonitoring May 08 – 15, 2012

Appendix B

Diffuser Discharge Concentrations of Total Residual Chlorine,

Diffuser Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion and Mollusks

During Toxicity Test Sampling

Page 15 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion

Mollusks, During Toxicity Test Sampling, March 12, 1998 – May 11, 2012

Date Sodium

Hypochlorite mg/L TRC

Towerbrommg/L TRC

PCL-222 mg/L

Phosphate

PCL-401 mg/L

Copolymer

CL-363 mg/L

DMAD

Cuprostat-PF

mg/L Azole

H-130Mmg/L Quat

03/12/1998 0.016 - - - - - - 03/13/1998 0.015 - - - - - - 03/14/1998 0.013 - - - - - - 03/15/1998 0.030 - - - - - - 03/16/1998 0.013 - - - - - - 03/17/1998 0.020 - - - - - - 03/18/1998 0.018 - - - - - - 09/08/1998 0.015 - 0.014 0.005 - - 0.021 09/09/1998 0.003 - 0.031 0.011 - - - 09/10/1998 0.014 - 0.060 0.021 - - - 09/11/1998 0.013 - 0.055 0.019 - - - 09/12/1998 < 0.001 - 0.044 0.015 - - - 09/13/1998 < 0.001 - 0.044 0.015 - - - 09/14/1998 0.008 - 0.044 0.015 - - - 02/22/1999 < 0.001 - - - - - - 02/23/1999 0.005 - - - - - - 02/24/1999 0.009 - - - - - - 02/25/1999 0.012 - - - - - - 02/26/1999 0.008 - - - - - - 02/27/1999 < 0.001 - - - - - - 02/28/1999 < 0.001 - - - - - - 08/18/1999 - 0.015 0.069 0.024 0.006 - - 08/19/1999 - 0.012 0.068 0.024 - - - 08/20/1999 - 0.023 0.070 0.024 - 0.120 - 08/21/1999 - 0.022 0.068 0.024 - - - 08/22/1999 - 0.022 0.068 0.024 - - - 08/23/1999 - 0.025 0.068 0.024 0.006 - - 08/24/1999 - 0.016 0.067 0.023 0.020 - -

Page 16 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During

Toxicity Test Sampling, March 12, 1998 – May 11, 2012

Date Sodium


Towerbrommg/L TRC

PCL-222 mg/L

Phosphate

PCL-401 mg/L

Copolymer

CL-363 mg/L

DMAD

Cuprostat-PF

mg/L Azole

H-130Mmg/L Quat

01/31/2000 - < 0.002 0.026 0.009 - - - 02/01/2000 - 0.011 0.026 0.028 - - - 02/02/2000 - 0.028 0.026 0.009 0.006 - - 02/03/2000 - 0.008 0.027 0.009 - - - 02/04/2000 - 0.006 0.027 0.009 0.005 0.109 - 02/05/2000 - < 0.002 0.027 0.009 - - - 02/06/2000 - < 0.002 0.027 0.009 - - - 07/26/2000 - < 0.0057 0.055 0.019 - - - 07/27/2000 - 0.019 0.055 0.019 - - - 07/28/2000 - 0.0088 0.053 0.018 0.004 0.108 - 07/29/2000 - < 0.0088 0.055 0.019 - - - 07/30/2000 - < 0.0076 0.055 0.019 - - - 07/31/2000 - < 0.0152 0.055 0.019 0.006 - - 08/01/2000 - < 0.0141 0.055 0.019 0.005 - - 12/11/2000 - 0.0143 0.025 0.020 0.005 - - 12/12/2000 - 0.0092 0.025 0.020 0.005 - - 12/13/2000 - < 0.0120 0.025 0.020 - - - 12/14/2000 - < 0.0087 0.025 0.020 - - - 12/15/2000 - 0.0120 0.025 0.020 0.005 - - 12/16/2000 - < 0.0036 0.025 0.020 - - - 12/17/2000 - < 0.0036 0.025 0.020 - - - 08/26/2001 - 0.017 0.06 0.021 0.006 - - 08/27/2001 - <0.0096 0.06 0.021 0.005 - 0.021 08/28/2001 - <0.0085 0.06 0.021 - - - 08/29/2001 - <0.0094 0.059 0.020 0.005 - 0.021 08/30/2001 - <0.0123 0.06 0.021 0.005 - - 08/31/2001 - <0.005 0.059 0.020 - - - 11/25/2001 - <0.0044 - - - - - 11/26/2001 - <0.0119 0.024 0.02 0.005 - - 11/27/2001 - 0.0137 0.023 0.019 0.007 - - 11/28/2001 - <0.0089 0.022 0.019 0.006 - - 11/29/2001 - 0.0132 0.024 0.02 0.007 - - 11/30/2001 - < 0.0043 0.024 0.02 - - - 12/09/2001 - <0.0042 - - - - - 12/10/2001 - <0.0042 - - - - - 12/11/2001 - <0.0104 - - - - - 12/12/2001 - 0.0128 0.024 0.02 0.008 - - 12/13/2001 - <0.0088 0.024 0.02 - - - 12/14/2001 - 0.0134 0.024 0.02 0.007 - -

Page 17 of 110



Date Sodium Hypochlorite

mg/L TRC

Towerbrommg/L TRC

PCL-222 mg/L

Phosphate

PCL-401 mg/L

Copolymer

CL-363 mg/L

DMAD

Cuprostat-PF

mg/L Azole

H-130Mmg/L Quat

01/02/2002 - < 0.0079 0.023 0.02 0.006 - - 01/03/2002 01/04/2002 01/05/2002 01/06/2002 01/07/2002

- - - - -

< 0.0042 0.0124

< 0.0042 < 0.0042 < 0.0089

0.023 0.024

- -

0.024

0.014 0.014

- -

0.014

- 0.009

- -

0.006

- - - - -

- - - - -

02/24/2002 02/25/2002 02/26/2002 02/27/2002 02/28/2002 03/01/2002

- - - - - -

< 0.004 < 0.004 0.0143

< 0.0041 < 0.0041 < 0.0041

- 0.023 0.023 0.023 0.024 0.024

- 0.023 0.023 0.023 0.008 0.008

- -

0.007 - - -

- - - - - -

- - - - - -

05/05/2002 05/06/2002 05/07/2002 05/08/2002 05/09/2002 05/10/2002

- - - - - -

- - - - - -

- 0.058 0.058 0.056 0.057 0.056

- 0.02 0.02 0.019 0.02 0.019

- 0.014 0.015

- 0.014

-

- - - - - -

- - - - - -

08/04/2002 08/05/2002 08/06/2002 08/07/2002 08/08/2002 08/09/2002

- - - - - -

<0.0058 <0.0058 0.0092

<0.0107 <0.0061 0.0152

- 0.053 0.053 0.055 0.055 0.054

- 0.018 0.018 0.019 0.019 0.018

- - -

0.007 -

0.008

- - - - - -

- 0.025

- - - -

10/06/2002 10/07/2002 10/08/2002 10/09/2002 10/10/2002 10/11/2002

- - - - - -

<0.00497 0.0153

<0.0092 0.0124 0.0134

<0.0042

- 0.054 0.054 0.053 0.054 0.054

- 0.018 0.018 0.018 0.018 0.018

- 0.009 0.007 0.009 0.009

-

- - - - - -

- - - - - -

01/12/2003 01/13/2003 01/14/2003 01/15/2003 01/16/2003 01/17/2003

- - - - - -

<0.0035 <0.006

<0.0118 <0.0063 <0.0034 <0.0034

- 0.025 0.026 0.026 0.026 0.026

- 0.019 0.020 0.020 0.020 0.009

- 0.009

0.009

-

- - - - - -

- - - - - -

04/06/2003 04/07/2003 04/08/2003 04/09/2003 04/10/2003 04/11/2003

- - - - - -

<0.0073 <0.0189 <0.0117 <0.0139 <0.0113 <0.0073

- - - - - -

- 0.021 0.021 0.021 0.021 0.022

- - -

0.016 0.018

-

- - - - - -

- - - - - -

Page 18 of 110

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced

Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 – May 11, 2012

Date Sodium


Towerbrommg/L TRC

PCL-222 mg/L

Phosphate

PCL-401 mg/L

Copolymer

CL-363 mg/L

DMAD

Cuprostat-PF

mg/L Azole

H-130Mmg/L Quat

06/15/2003 - < 0.0045 - - - - - 06/16/2003 06/17/2003 06/18/2003 06/19/2003 06/20/2003

- - - - -

< 0.0037 < 0.0048 < 0.0048 < 0.0085 < 0.0048

0.057 0.041 0.041 0.058 0.058

0.020 0.014 0.014 0.020 0.020

- - - - -

- - - - -

0.022 0.024 0.024 0.025 0.025

08/03/2003 08/04/2003 08/05/2003 08/06/2003 08/07/2003 08/08/2003

- - - - - -

<0.0050 <0.0050 <0.0051 <0.0084 0.0129 0.0153

- 0.058 0.057 0.057 0.057 0.057

- 0.020 0.020 0.020 0.020 0.020

- - - - -

0.009

- - - - - -

- -

0.025 0.025 0.024

-

10/05/2003 10/06/2003 10/07/2003 10/08/2003 10/09/2003 10/10/2003

- - - - - -

<0.0043 <0.0043 <0.0090 <0.0106 0.0181 0.0183

0.057 0.057 0.057 0.057 0.026 0.026

0.020 0.020 0.020 0.020 0.022 0.024

- - - - -

0.009

- - - - - -

- 0.025 0.025 0.025 0.025

-

02/01/2004 02/02/2004 02/03/2004 02/04/2004 02/05/2004 02/06/2004

- - - - - -

0.0093 <0.0034 <0.0034 0.0124

<0.0034 0.0105

0.027 0.026 0.026 0.026 0.026 0.026

0.009 0.009 0.009 0.009 0.009 0.009

- - -

0.009 -

0.010

- - - - - -

- - - - - -

05/04/2004 05/05/2004 05/06/2004 05/07/2004 05/08/2004 05/09/2004

- - - - - -

<0.0123 <0.0144 <0.0146 0.0227 0.016

<0.0104

0.026 0.026 0.037 0.058 0.060 0.058

0.019 0.014 0.013 0.020 0.021 0.020

- 0.009

- 0.009

- -

- - - - - -

0.025 0.025 0.025 0.025

- -

07/04/2004 07/05/2004 07/06/2004 07/07/2004 07/08/2004 07/09/2004

- - - - - -

0.0217 <0.0085 <0.0077 0.0252 0.0223 0.0182

0.057 0.057 0.058 0.056 0.057 0.057

0.019 0.020 0.020 0.019 0.019 0.020

- 0.009

- -

0.009 0.009

- - - - - -

- -

0.031 0.031

- -

Page 19 of 110



Date Sodium

Hypochlorite

mg/L TRC

Towerbrom mg/L TRC

PCL-222 mg/L

Phosphate

PCL-401 mg/L

Copolymer

CL-363 mg/L

DMAD

Cuprostat-PF mg/L Azole

H-130M mg/L Quat

Nalco 73551 mg/L

EO/PO

H-150M mg/L Quat

11/07/2004 11/08/2004 11/09/2004 11/10/2004 11/11/2004 11/12/2004

- - - - - -

<0.0187 <0.0192 <0.0233 <0.0149 <0.0149 <0.0253

0.000 0.047 0.048 0.047 0.049 0.048

0.014 0.030 0.016 0.016 0.017 0.017

- - - - - -

- - - - - - - - -

- 0.041 0.041 0.043 0.042

- - - - -

- - - - -

02/06/2005 02/07/2005 02/08/2005 02/09/2005 02/10/2005 02/11/2005

- - - - - -

<0.0042 <0.0116 <0.0080 0.0199

<0.0042 0.0155

0.028 0.028 0.028 0.028 0.028 0.028

0.010 0.010 0.010 0.010 0.010 0.010

- - - - - -

- - - - - -

- - - - - -

- 0.007

- - -

0.007

- - - - - -

06/05/2005 06/06/2005 06/07/2005 06/08/2005 06/09/2005 06/10/2005

- - - - - -

0.0063 0.0043 0.0103 0.0295 0.0129 0.0184

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- 0.037 0.037 0.037

- -

07/17/2005 07/18/2005 07/19/2005 07/20/2005 07/21/2005 07/22/2005

- - - - - -

0.0109 0.0150 0.0163 0.0209 0.0242 0.0238

0.026 0.026 0.026 0.026 0.026 0.054

0.009 0.009 0.009 0.009 0.009 0.018

- - - - - -

- - - - - -

- - - - - -

- - -

0.014 -

0.014

- 0.036 0.036 0.036

- -

10/30/2005 10/31/2005 11/01/2005 11/02/2005 11/03/2005 11/04/2005

- - - - - -

0.0068 0.0112 0.0104 0.0104 0.0117 0.0165

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- -

0.035 0.036 0.036 0.035

11/14/2005 11/15/2005 11/16/2005 11/17/2005 11/18/2005 11/19/2005

- - - - - -

0.0274 0.0256 0.0234 0.0231 0.0200 0.0116

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

Page 20 of 110

Table B-1 (continued). Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Growth of Microbiologically Induced

Bacteria and Mollusks, During Toxicity Test Sampling, March 12, 1998 – May 11, 2012

Date Sodium


Towerbrommg/L TRC

PCL-222 mg/L

Phosphate

PCL-401 mg/L

Copolymer

CL-363 mg/L

DMAD

Cuprostat-PF mg/L Azole

H-130M mg/L Quat

Nalco 73551 mg/L

EO/PO

H-150Mmg/L Quat

MSW 101

mg/L Phosphate

11/12/2006 11/13/2006 11/14/2006 11/15/2006 11/16/2006 11/17/2006

- - - - - -

0.0055 0.0068 0.0143 0.0068 0.0267 0.0222

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- 0.037 0.037 0.037 0.037

-

- - - - - -

11/26/2006 11/27/2006 11/28/2006 11/29/2006 11/30/2006 12/01/2006

- - - - - -

0.0188 0.0138 0.0120 0.0288 0.0376 0.0187

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

05/28/07 05/29/07 05/30/07 05/31/07 06/01/07 06/02/07

- - - - - -

- -

0.0084 0.0103 0.0164 0.0305

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- -

0.017 -

0.017 -

- 0.036 0.036 0.036 0.036

-

0.015 0.015 0.015 0.015 0.015 0.015

12/02/07 12/03/07 12/04/07 12/05/07 12/06/07 12/07/07

- - - - - -

0.0241 0.0128 0.0238 0.0158 0.0162 0.0175

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - -

04/13/08 04/14/08 04/15/08 04/16/08 04/17/08 04/18/08

- - - - - -

0.0039 0.0124 0.0229 0.0143 0.0120 0.0149

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

10/26/08 10/27/08 10/28/08 10/29/08 10/30/08 10/31/08

- - - - - -

0.0260 0.0151 0.0172 0.0154

- 0.0086

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- 0.017

- 0.018

- -

- -

0.041 0.041 0.041 0.041

- - -

0.030 0.030 0.030

Page 21 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test

Sampling, March 12, 1998 – May 11, 2012

Date Sodium


Towerbrom mg/L TRC

PCL-222

mg/L Phosph

ate

PCL-401 mg/L

Copolymer

CL-363mg/L

DMAD

Cuprostat-PF mg/L

Azole

H-130Mmg/L Quat

Nalco 73551 mg/L

EO/PO

Spectrus CT1300

mg/L Quat

H-150M mg/L Quat

MSW 101

mg/L Phosphate

02/08/09 02/09/09 02/10/09 02/11/09 02/12/09 02/13/09

- - - - - -

0.0197 0.0237 0.0104 0.0155 0.0106

-

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

0.017 0.017 0.021 0.017 0.017

-

- - - - - -

- - - - - -

- - - - - -

05/10/09 05/11/09 05/12/09 05/13/09 05/14/09 05/15/09

- - - - - -

0.0129 0.0415 0.0053 0.0049

<0.0141 <0.0160

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- 0.0446 0.0396 0.0396 0.0397

-

- - - - - -

11/15/09 11/16/09 11/17/09 11/18/09 11/19/09 11/20/09

- - - - - -

0.025 0.0152 0.0255 0.0306 0.0204 0.0093

- - - - - -

- - - - - -

- - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

05/09/10 05/10/10 05/11/10 05/12/10 05/13/10 05/14/10

- - - - - -

0.0192 0.0055 0.0100 0.0171 0.0041 0.0099

- - - - - -

- - - - - -

- - - - -

- - - - - -

- - - - - -

- - - - - -

- -

0.039 0.039 0.039 0.039

- - - - - -

- - - - - -

Page 22 of 110

Table B-1. Sequoyah Nuclear Plant Diffuser (Outfall 101) Discharge Concentrations of Chemicals Used to Control Microbiologically Induced Corrosion Mollusks, During Toxicity Test

Sampling, March 12, 1998 – May 11, 2012

Date Sodium


Towerbrom mg/L TRC

PCL-222

mg/L Phosph

ate

PCL-401 mg/L

Copolymer

CL-363mg/L

DMAD

Cuprostat-PF mg/L

Azole

H-130Mmg/L Quat

Nalco 73551 mg/L

EO/PO

Spectrus CT1300

mg/L Quat

H-150M mg/L Quat

MSW 101

mg/L Phosphate

10/31/10 11/01/10 11/02/10 11/03/10 11/04/10 11/05/10

- - - - - -

- 0.0122 0.0112 0.0163 0.0107 0.0132

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

05/01/2011 05/02/2011 05/03/2011 05/04/2011 05/05/2011 05/06/2011

- - - - - -

- - -

0.0155 0.0179 0.0089

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- 0.04 0.04 0.04 0.04

-

- - - - - -

- - - - - -

11/06/2011 11/07/2011 11/08/2011 11/09/2011 11/10/2011 11/11/2011

- - - - - -

0.0168 0.0225 0.0141 0.0239 0.0242 0.0231

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

05/06/2012 05/07/2012 05/08/2012 05/09/2012 05/10/2012 05/11/2012

- - - - - -

- - -

0.0145 0.0298 0.0174

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- - - - - -

- -

0.041 0.041 0.041

-

- - - - - -

- - - - - -

Page 23 of 110


Appendix C

Chain of Custody Records and Toxicity Test Bench Sheets

Page 24 of 110

of 110

---.-., .----

"... . ..'

BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of _1_

Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

Project Name: Sequoyah NP Toxicity 351 Depot Street. FedEx UPS Bus Client 0 Urier)

P,O, Number: N/A Asheville, NC Other (specify):

28801 :;JraIComments: A......i"ul r'~ ~I fJjSS,Facility Sampled: Sequoyah NP~~,r/...4 .-1- O1JCl o.-L '

NPDES Number: TNOO26450 Phone: 828-350-9364 S.~ i3 JiJ n ~/Je,c} J,.;II~ rt-Z3.Collected By: 'lNs/-:" 8,'~<- tb,~

}

Fax: 828-350-9368Lynk.ll frI;//'~ P """t ...1-~

i:~~~}'8~-:,,' -

- ,

Container ' " ~~.

". c~.

Ficldldentification I Numbt:r& Flow ..~

Grab/Comp. Collection Dateffime Rain Event? ..;.", .;

. Laboratp~; lIse .~Sample Description Volume (MGD)(Mark os Appropriate) ~::" .... ~,

~" '=0 ' "- Date Time . . . Yes IfVcs, No Trace ~mLog Airival Temp. ' By_,- Time :" Appe:ar-

I\j ~ - - ' ~ : Inches .' :'Number . '. ("0'- . ~'(' , ance

~lrOIg ...... w-~~ X I~.cil ;'~ '~Jt ~: l-tu\ i--SQN-IOI-TOX Comp ~S ....2(2.5gal) "~ '~..,?,'~t:''=: :](2.5 gal) JJ;fl

>ii¢iH:02. ' t.. (-t1.'\ .~SQN-INT-TOX Comp I .. O-C·-~ ,.,:,' .' .: . ,

Sample Custody - Fill In From Top Down 1- (.\lS~~ <;'U'l1S ' ....Tl\tt. Si\,",'I.4!S ~cel

Dale/Time Received By (Signature):IN"" .. ~""..I<l. _\ "-':::, ~;~..I'\u.Relinquished By (Signature): Dateffime

~IJ~a . IlJ t. ~-O~ J-~ 7-/2. /0)0 E.~ ;f?, f( 52 j.,' (J..,_Sol'o)\(.. '-/-

, ',,,-.• J f) --"~f.,,/ O:r'o? _ J ;;), / lJ:,17:> £.1"

$.1<,.~/ - ~\c....0':.' (/"\.l'\... ''\<.G E'\ (\Ut( Iv-- ' E'TS

\'-\7..,\O':>-<r\- 11... e,-~f

Instructions: Clients should fill in all areas except those in the "Laboratory Use" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in icc. The hold time for each sampleis 36 hours from the time of collection. Therefore; please collect and ship in such a way that the laboratory will receive the samples willI ample time to initiate testing within that time frame. Samplesshipped O'vcmight on Friday via FedEx or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

of 110

@~iETS~.. ~5o/ullo.... lnc.'filJ£_ll

Whole Emuent Toxicity Sample Receipt LogPage -rz.-

*Sample temperature perfOl:med using Samp\eReceiving Thermometer: SN 633.8.

nat< Time Received Received Sample Project Sample Sample DBIDe and desCription State Comments

~""'" ...-... by 'rom temperature ('C)" numberOS..Q7~12 142' J. Sumner TVA Courier 1.8/1.8 787S 120507.01 TVA-S " Nuelear Plant - Outfall 101 1N05-07-12 1429 J. Sumner TVA Courier 1.0 7875 120507.02 TVA-S Nuclear Plant - Intake 1NOS-Q7-12 1451 J. Sumner TVA Courier 1.7 7876 120507.03 TVA - Watts Bar Nuclear Plant - Outfall101 1N05-07-12 1451 J. Sumner TVA Courier 3.7 7876 120507.04 TVA - Watts BarNuclearPiant- Intake 101 1N05-07-12 1451 J. Sumner .TVA Courier 2.' 7816 120507.05 TVA -Watts Bar Nuclear Plant - Outfall 113 1NOS-oH2 1451 J. Sumner TVA Courier 3.5 7876 120507.06 TVA - Watts Bar Nuclear Plant· Intake 113 1N

SOP G4 - Exhibit 04.2, revision 01-03·12

of 110

_.- ._.- ._--- ------

'. ~ .BIOMONITORING CHAIN OF CUSTODY RECORD Page __l __ of __l__

Client: TVA Environmental Testing Solution, Inc. Delivered By (Circle One):

Project Name: Sequoyab NP Toxicity 351 Depot Street. FedEx ups Bus Client ~P,O, Number: N/A Asheville, NC Other (specify):

().r:,:;S

Facility Sampled: Sequoyab NP 28801 GeneraIComments:~;~ ....f 0"W:-/1..-r-b'6~:;'

Phone: 828-350-9364,..,..I.. -I- iM-..k<- -+ ~:zs:

NPDES Number: TNOO26450

Collected By: Dustin Bi~n il~ ~" Fax: 828-350-9368L'ftlck.J1 ,} ~M.s ' ;

Container

:i;~~~l::;ii1berut~~'U::·"~~:'~).·'·:::' ';:, " ',.Field Identification I Number & Flow Rain Event?Sample Description

Grab/Comp, Collection DateITirne Volume (MGD)Collected (Mark as Appropriate)

- Dale Time, ",' Yes IfYC3. No Trace , &-510& ' }Jii,,81~Temp. \ , B\j , 'Time~ ' .. ,Appear- .• - ·NU01~;:·~~' -:~ E."f' . ., , Inches c' '''(·0) ,: , . "alice ,~ ~_ 't'". ....,.

~;:- I:-+' ,"SS .-< ~/S.?, r/'-E: 1~9.;lS' :t:~;c. h:~~ ;r.;. . ... ,~

SQN·IOI-TOX Comp o n f.,. 0$55. r" 2(2.5gal) ..". . /' "fiu,ci: X . \.~OO >1:." ; fj'·',,,,·:-... . '

SQN-INT-TOX Comp 'J/D~Z' \--.-t 0"1-/6 ~I(2_5 gal) IJ/,q X lib~%I, r'i¥' .. :" .:M- ',,;~' -~ -11:-DS/~}l. ,..". 0 ../0 '" · •.lD C-

_. :~h~-( _:'-"'~:_ :,c,,,,·,;,: -'.', !-' - ~. l.eX:.. ·

Sample Custody - Fill In From Top Down ~ ~t>" ~l.S 1'"""-.", ~Wl.e!; ~c.ellieb

I" """.. (J)..u\ ..o~. ~,...._ IN :''''-

Relinquished By (Signature): DatelTime Received By (Signature): DaleITime d',

_.L''J \VA $ON\C tJ~tJ'f.. J71/lui;;. f'Y-.n, I <.. J-/df)- fj f{, ~~O_"-:-::-"7-;0 .? O'Y>/5 e-t" t1li"- I'::'-~

~R 2..4-P'-J.::o~C

<J s .... D"'1- / '- 'la tJJE.- r \Iv------ c'S 0$-6'\-1<_ \~C £i'-.../

Instructions: Clients should fiU in all areas except those in the "Laboralory Use" blod(. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sampleis 36 hours from the time of collection. Therefore. please collect and ship in such a way that the laboratory will receive the samples with ample time to initiate testing within that time frame. Samplesshipped overnight on Friday via FedEx or UPS must be marked for Saturday delivery or the)' will not arrive until the following Monday.

'I'

of 110

ldHI~~}

iETS-6 EnvI_1T_So/utIo.... _

Whole Effluent Toxicity Sample Receipt Log·page~

"'Sample temperature performed using Sample Receiving Thermometer: SN 6338.

Date TIme Reteived Received Sample Project Sample Sample Dame lind description State Comments

received ....w.d bv from temperature ('C)" numberOS.()9-12 1002 KKomm Fed-Ex 1A 7898 120509.01 CORRERI~AAFM " Inmrnational NCOS.()9-12 1002 KKomm Fed-Ex 2.0 7899 120509.02 Bladenboro WWTP NC05-09·12 1002 KKomm Fed-Ex 1.7 7900 120509.03 Duke En - Belews Creek Steam Station NC05-09-12 1002 KKomm Fed-Ex 1.5 7901 120509.04 Pro essEn -C eFearS.E. NC05-09-12 1002 K.Keenan Fed -Ex 1.5 7901 120509.05 Pro ,Enu e Fear S.E. - U "1,, NC05-09-12 1002 KK~ Fed-Ex 0.7 7902 120509.06 Ci of Gastonia - Dallas WWTP NC05-09-12 1002 K.Keenan Fed- Ex 5.' 7903 120509.07 United Water - Enfield WWTP NCOS..()9-12 1002 KKomm Fed-Ex 2.3 7904 120509.08 Duke En - Marshall Steam Station NC05-09-12 1002 KKomm Fed-Ex I., 7905 120509.09 Ammons Resources - Ralei CC NC05..09-12 1002 K.Keenan Fed,~Ex 2.6 7906 120509.10 B,O rises - Scarlett Acres MHP NCOs..o9-12 1002 KKomm Fed -Ex 5.' 7907 120509.11 United Water - Scotland Neck WWlP NC05-09-12 1002 KKomm Fed -Ex 1.7 790. 120509.12 Duke En - McGuire NS - Outfall 005 NC05-09-12 1002 KKomm Fed-Ex 2. 790' 120509.13 Duke En ~ McGuire NS - Outfall 001 NC05-09-12 1002 K. Keenan Fed -Ex 1.7 7910 120509.14 Duke En ~ McGuire NS - Outfall 002 NC05-09-12 1002 K. Keenan Fed· Ex 1.5 7911 120509.15 Elementis NC05-09-12 1002 KK~ Fed-Ex 1.1 7912 120509.16 Carolina Beach WWlP NC05-09·12 '020 K. Keenan UPS 0.' 7913 120509.17 SouthC WWTP NCOS-09-12 1020 K.Keenan UPS 5.' 7914 120S09.18 Enviro Chemists - Manteo WWTP NC05-09-12 1020 K.. Keenan UPS 2.0 7915 120509.19 Microbae Environmental· Rockin WWTP NC05-09-12 1020 K.Keenan UPS 2.0 7916 120509.20 Microbae Environmental- Roseboro WWI'P NCOs..o9-12 1020 KK..... UPS 2.0 7917 120509.21 Microbae Environmental- JP Stevens· W WWTP NC05-09-12 1020 KKomm UP, 2.0 7918 120509.22 Microbae Environmental- TransMontainge· Outfall 002 NCOS-09-12 1020 K. Keenan UPS 2.0 7918 120S09.23 Microbac Environmental- TransMontain - Outfall 003 NC05-09-12 1125 KK~ Dash Courier 1.2 7919 120509.24 OWASA NCOS-09-12 1300 J. Sumner TVA Courier 1.611.7 787S 120S09.2S TVA-S " NuclearPlant-Outfal1101 TNOS-{)9-12 1300 1. Sumner TVA Courier 1.6 71175 120509.26 TVA-S uoyah Nuclear Plant - Intake TN05-{)9-12 1522 1. Sumner TVA Courier 2.2 7876 120S09.27 TVA - Watts Bar Nuclear Plant - Outfall 101 TN05-09-12 1522 J. Sumner TVA Courier I., 7876 120509.28 TVA - Watts Bar Nuclear Plant - Intake 101 TN05-09-12 1522 J. Sumner TVA Courier 2.5 7876 120509.29 TVA - Watts Bar Nuclear Plant - Outfall 113 TN05-09-12 1522 J. Sumner TVA Courier I., 7876 120509.30 TVA - Watts BarNuclear Plant - Intake 113 TN

SOP 04 - Exhibit 04.2, revision 01-03-12

of 110

..BIOMONITORING CHAIN OF CUSTODY RECORD Page _1_ of_l_

eClient

Other (specify):

Delivered By (Circle One):

FedEx UPS Bus

General Comments:,tl-ri~ .....+evtf-/f ....+05S5"""" ".,j.. ;nl-k- -f 0630.

28801

828-350-9364

828-350-9368

Environmental Testing Solution, Inc.

351 Depot Street.

Asheville, NC

Collcction Date/rime

~~2'Comp

Grab/Comp.

SQN·INT-TOX

Field Jdentification ISam.ple Description

Facility Sampled: Sequoyah NP

P.O. Number: N/A

Project Name: Sequoyah NP Toxicity

Client: TVA

NPDES Number: TNOO26450 I Phone:

Collected By: Dustin Bige~"'i'II~ fJ-.J

L"n.Je)( \.-.h ,pM.s,.::::z~i!I1l =:::;;E;;;;:,-"T""---r---""T"-----.L--,.~~~~~~~"":'""'~~::__"":'""'...___J"QlY:l):.Ql;f qe>-:)'5' 0:';';'.-1<, . T .~'''' 0",·

(~~~) Rain Event? . :;,;:;:f~fq·.;:€,' ': La~::'" 0;e:' ;'/~::.'~); ..;-(Mark as AppropnaIe) ::t:-',,;..~t.<?,;,:" ... ,~~ • ~ • _ ry "', ,> ''', - ~

•. "'':--'~'''::~;:''. -.".. ~..Y

Dale Time IfYcs. No Trace ~SJ..og~'" :~:Nrival Temp: ,By.',. rime :;': :r;A'ppear·Inches ..::~~:i'_ .. : . . (0 "'.-:: _ .:~' .:... 'f...'V _~.. ·':ance·

)(.. r§:~.l;'i:o 1J~'(. {OlJ'b .~< .~~i~.:, c~i-

y... \~fI~. ';'H·(..~> .;~;, ':>~;C<*' ".Sample Custody - Fill In From Top Down ,., L~"","'-\ ':>eA\!o I~. ~",I'\.eS (I~

DateffimeRelinquished By (Signature):'''' €oooll U>"l>'~>l. ~ Ht.!>W"l '" flu.

Daterrime 61u.---

0"-1\- 11... \1."tS e..-

0'8,3 0 Eo,os j/, /z

ETS

50 p..l\L

Received By (Signature):

ft~t<l"i-<\ ('r/~

oH,., ~.£I tt U; ,.1,'5op..l\(.\oS-I/-/2.

, TV.... ,h'Y 'o$,/ZO/,2.0,.....

B~.2L.e"

Instructions: Clients should fill in all areas except those in the "Laboratory Usc" block. Biomonitoring samples are preserved by storing them at 6°C and shipping them in ice. The hold time for each sampleis 36 hours from the time of collection. 'Therefore, please collect and ship in such a way thai. the laboratory will receive the samples with ample time to initiate testing within that time frame. Samplesshipped overnight on Friday via FedEx. or UPS must be marked for Saturday delivery or they will not arrive until the following Monday.

of 110

i;::>""~ ~iil

iET'S6· E......-TestlngS.....-.ln'"

Whole Emuent Toxicity Sample Receipt LogPa",,_:lJo

tSampletemperature perfimned using Sample Receiving Them1ometer: SN 6338.

Dale Time Ref:eived Ro"""" Sample Project Sample Sample name and description State CommentsIe_"""'" bY from temperature ('q" number

05-11-12 1014 K K<enan Fed -Ex 0.7 7898 120511.01 CORRERI-AAFM Intemational NC05-11·12 1014 K. Keenan Fed -Ex 1.0 7899 120511.02 Bladenboro WWTP NC05-lH2 1014 K. Keenan Fed-Ex 1.6 7900 120511.03 Duke Enetl1Y - Belews Creek Steam Station NCOS-Il-12 1014 KK~ Fed-Ex 0.4 7901 120511.04 Pro ssEn - eFear S.E. NC05-11-12 1014 K. Keenan Fed-Ex 0.' 7901 120511.05 Pro ssEn -C e Fear S.B. - U stream/Intake NC05-11-12 1014 Ie. Keenan Fed-Ex U 7902 120511.06 Ci ofGastonia - Dallas WWlP NC05-11-12 1014 K. Keenan Fed-Ex 1.5 7903 120511.07 United Water· Enfield WWTP NC05-11-12 1014 KKooo.. Fed-Ex I., 7904 120511.08 Duke En - Marshall Steam Station NC05-11-12 1014 KKooo.. Fed-Ex 1.2 79<J5 120511.09 Ammons Resources - Ralei CC NC05-11-12 1014 K.Keenan Fed -Ex 22 79<J6 1205lUO Bol rises - Scarlett Acres MHP NC05-11-12 1014 K.lCeenan Fed -Ex 1.5 79<J7 120511.11 United Water· Scotland Neck WWTP NC05-1H2 1014 K K<enan Fed-Ex 0.9 7908 12051U2 DukeEn - McGuire NS - Outfall 005 NC05-11~12 1014 K K<enan Fed-Ex 1.0 7911 12051LJ3 Elementis NCOS-l1~12 1014 KK~ uPS 0.8 7913 120511.14 Srn>thC WWIP NC05~1l-12 1014 K.Keenan UPS 0.8 7915 120511.15 Microbac Environmental - Rockin ~WWIP NC05-11-12 1014 K. Keenan UPS 0.8 7916 120511.16 Microbac Environmenta1 - Roseboro WWTP NC05~1l-12 1014 KK~ UPS 0.8 7917 120511.17 Microbac Environmental· Jp Stevens _W WWIP NC05-11-12 1014 KKooo.. UPS 0.8 7921 I20511.18 Microbac Environmental - Motiva ses,LLC NC05-11-12 1123 K.Keenan Dash Courier 0.9 7919 120511.l9 OWA$A NC05-1I-12 1225 J. Sumner TVA Courier 0.510.6 7875 120511.20 TVA-S ah Nuclear Plant ~ Outfall 101 IN05-11-12 1225 J. Sumner TVA Courier 21 7875 120511.21 TVA - S u 3h Nuclear Plant - Intake IN05-11-12 1437 J. Sumner TVACowier 3.6 7876 120511.22 TVA- WattsBarNuclearPIant-OutfalIIOl IN05-1H2 1437 J. Sumner TVA Courier '4.5 7876 120511.23 TVA ~ Watts Bar Nuclear Plant ~ Intake 101 IN05-1H2 1437 J. Sumner TVA Courier 2.' 7876 120511.24 TVA - Watts Bar Nuclear Plant - Outfall 113 IN05-1H2 1437 J. Sumner TVA Courier 5.8 7876 120511.25 TVA - Watts Bar Nuclear Plant - Intake 113 IN

SOP 04 - Exhibit 04.2, revision 01-03-12

of 110

Page 1 of6

County: RheaOutfall: 101

I----------f-l!~~~ "'"'0,,\10

I-----------+=~=-.:::..-..-I

pH = , .'"'\~ S.U.Tern erature = 1.."1.a °C

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)Species: Pimephales promelas

Client: Tennessee Valley AuthorityFacility: Sequoyah Nuclear PlantNPDES #: TN0020168Project #: -'--I-Q&f-I1L.woS.a------

Transfer bowl information:

Average transfer volume:

Dilution preparation information: . Comments:Dilution prep (%) 10.8 21.6 43.2 86.4 100Effluent volume (mL) 270 540 1080 2160 2500Diluent volume (mL) 2230 1960 1420 340 0Total volume (mL) 2500 2500 2500 2500 2500

Daily feeding and renewal information:

Average weight per surviving larvae:

MHSWbatchused

Sample numbers used

SOP AT20 - Exhibit AT20.3, revision 06-01-11

Test initiation,renewal, ortermination

Time Anal st

Acceptance criteria

Afternoon feeding

Time

Morning feedingDate

Control in ormation:% Mortality:Avera e wei t er initial larvae:

o

Day

of 110

IndOjlend,ntI\OI'i<wiJy

."'tit". SOP AT20 - Exhibit AT20.3, revision 06-01-11

Day CONTROL 10.8% 21.6%

A B C D E F G H 1 J K L0

10 10 (0 10 10 fO 10 10 10 10 '0' /0.1 /0 10 ID 10 10 10 If;) 10 10 10 /0 102

10 10 (0 10 10 /0 IC IC 10 10 '0 103

1"0 /0 10 10 to 10 /0 10 10 10 10 104

10 10 Ie> Ie 10 10 10 10 Itl 10Itl 105

10 10 10 10 10 10 10 "~ 1010 10 106 Ie to 10 10 10 10 10 ID 10 10 '\ 107

Ie 10 10 10 '0 10 10 10 10 'I 1010A - Pan weight (mt

1'Jl",Tray color code:: W~

\~.,'i 'L·tS 14.•~ 13·14 14. (.,0; lo;.~4 '+'of '3.3'1 \Zo.91. 14.", 14-.Di ,4-."Analyst: MDate: 0$.07...11.

B - Pan + Lan:ae w1ht (mg)

2D,Z,1- 1'f.1t41-\,1.'1 2./,'15 ~.&f I'!·ett I'U~ 2J.Tl ZO·Ol 'lJ.t:t-Analyst: {l~ 22-01- 11,011Date: O!F.I'LC - Larvae weight (mg) - B - A

c..SS c.. ll.1o '.0 ,.,g '-."t-l vt& Co fI"\ 10.'\"\ 10.1.'\ 1.n 5."\'1 ,.11.Hand calculated.........\ f..Analyst:

Weight p~r initial number of larvae (mg)= C / Initial number of larvae

~ "':>... ~ ~/'0.,.

~ '" &0.,'::1.,Hand calCUlated. oJ ",,;F $' ,,(;- ,)" ~ ~\jl \I \0 '" \II I'i "Analyst: ~

O· "0' r:! ()' O' I)' 0' O· 0' O' (l' I)'

Averageweigbt per Percent reduction

'.'57.initial number of from control (%) 0.'01 O,c..~ o. 1010'-\ 1.0. \i.larvae (mo)

Page 2 of6

Date:

Survival and Growth Data

Species: Pimephales promelasClient: TVA I SeQuoyah Nuclear Plant, Outfall 101, Non-treated

Comment codes: c ~ clear, d = dead, fg = fungus, k = killed, m = missing, sk ~ sick, sm = unusually small,Ig =unusually large, d&r = decanted andreturned, w =wounded.

.

Comments:

i""f¥i:;;gn:",~

••I :

.~ =E"'-'~-"~-'"-talTo-..-trng-cso-'",-',"-",-",

IIIIIIIIIIIIIIIII

of 110

IIIII,

I,II,I~~

I,I"I

Page 3 of6

Species: Pimephales promelasClient: TVA / Se9uoyah Nuclear Plant. Outfall 101. Non-treated Date: oS·at· \J-

Survival and Growth DataDay 43.2% 86.4% 100%

M N 0 p Q R S T U V W X0

10 10 10 10 10 1(;) 1(:) 10 1010 10 (01 Ie 10 10 10 I() If) It) It) 10 10 10 102 I/;) 10 10 10 '0 10 10 10 '0 10 /CJ 103 10 10 10 10 It> 10 /0 10 Ie (010 /D4 IQ /0 /0 (0 ID /0 10 ID 10 10 10 105

ID ID to /0 It) /0 10 /0 10 10 '0106 10 10 10 10 10 ID 10 /0 10 10 1'0 107

10 10 Ie 10 /(:) I() 10 If) /0 Ie1(:) 10A - Pan weight (mgLTray color code:: Itt\. Yi...

If.~o 14.t.'I 14:n Il.,4S 1'3·ft ''3·l.t I!.ol \'3.1.4- I~.t!> 14·'18 14-.t.S 1~.~8Analyst: mDate: 0S."01A'L

~;a~~~:+Lar~rht (mg)

121·0& 'Wf3 ll.l't ''1.u. 'lD.5'Z- 1'i,"H ".W Iq.ll·~ Itt.lflf 1J.!Z. 20.51 ~o.110Date: t6.\. .C,... Larvae weight (mg) - B - A

Hand calculated. ,j Io.,g 10.'" ",.\{t. Co.'''' 10.1,,0 Co,b'l ",.!>' "',.. ~ c... '1 \ Io •.,,~ 5.(tlo"'.~1-

Analyst: " ..

Weight per initial number of larvae (mg)= C I Initial number oflarvae

~ ,,:>.. ,,~ .:>.,q. No J- ~ .J>'l/~ ~O f;):>" .:-Hand calculated.

01- '" '0 '11 '" \Ii '" \0 \II ':)Analyst: O· '" o· tl' O' 0'O· \:)' O' O· O' O' O'Average weight per Percent reduction

o.r..bi S.1 l. O."''-1'2.. '\,'2.7•. o.\,,'M ID. &7"initial number of from co~trol (%)larvae (mil)

Comment codes: c = clear, d ~ dead, fg = fungus, k = killed, m = missing, sk ~ sick, sm = unusually small,19 ~ unusually large, d&r ~ decanted and returned, w ~ wounded.

Comments:

Ind..,.ndon,ll<vIewbv

'''R"' SOP Ar20 - Exhibit Ar20J, revision 06'01-1 1

of 110

Species: Pimephales promelasClient: TVA I Seguoyah Nuclear Plant. Out[alll0l. Non-treated Date: os.at- I'l-

Page 4 of6

\.&7.

Survival and Growth Data100% Intake

y Z AA BB

10 10 10 10

Ie 10 10 10

10 ID If) 10

10 10 10 10

IQ 10 to to10 ID ID toIt) ID IC) 10

It If) 10 10

14:.\ o'M3 13.'to 05.',

1l.SS 1'Ia-1- 10•.,3 2O.lor

,.'!M l..i-\ 1o.4\~ ,.lb

o

Day

Environmental Testing Solutions, Ine,

ET

5

4

A = Pan weight (mgLTray color code:: *"0n~"l-,---,=t',wl...~-~Analyst: Mu:t:-Date: 0,&'01- \1<

1

B = Pan +~Larvawei t(.mg)Analyst: _-,- _

Date: • , -

C = Larvae weight (mg) = B - A

7

2

3

6

Hand calculated. I ~ ...Analyst: ~,.•-- -

Weight per initial number of larvae (mg)= C I Initial number of larvae

Hand calculated. !!'Analyst: . cp'"Average weight per Percent reductioninitial number of from control (%)larvae (m )

Comment codes: c =clear, d = dead, fg = fungus. k = killed. m =missing, sk = sick, sm = unusually small.Ig = unusually large, d&r = decanted and returned, w = wounded.

Comments:

ImloF"'ndonl

~=.K.l':l~: ~...n

~i:


of 110

I ~H,"nTVA I Sequoyah Nuclear Plant, Outfall 101

Non-treatedMay 08-15, 2012

7875Project number:

Pimephales promelas Chronic Whole Effluent Toxicity TestEPA-821-R-02-013, Method 1000.0

Quality ControlVerification of Data Entry, Calculations, and Statistical Analyses

Environmental Testing Solutions, Inc, ._.-Not for Com liance Assessment, Internal Laborato QC

Cwn:elltratioD (%) .R~pu...te lDifilll nmber of Fin.lllumber ofllln'ae A-Pan weight (mg) B m Pan + Larvae Larvae weight (mg) Weight I SQl'\iving Mellllwelghtl CoefliCiellt ofvariatloll Weight I IDillalllumber Mean nrvival Mean weig'! I IlIltl.ol CoefIielenf of I'eJftnt redaction fromlarvae welght(mg) =A_B ...mberofla""lle(mg) S.....lvlng umber of (M..n ..""" ......._~ ofJarvu(mg) "" .umber "'larvae ....ri.tioD (Mt......... e..afrol (%)

larvae (mg) ........... ,......)(%) (mg) .......'ol ....b<r.r ....ooj

%

A 10 10 l3.69 20.27 6.58 0.658 0.658

ControlB 10 10 12.78 19.64 6.86 0.686

0.707 7.20.686

100.0 0.707 7.2 Not applicableC 10 10 14.17 21.24 7.07 0.707 0.707D 10 10 13.67 21.45 7.78 0.778 0.778E 10 10 14.65 20.89 6.24 0.624 0.624

10.8%F 10 10 15.54 22.02 6.48 0.648

0.655 4.'0.648

100.0 0.655 4.' 7.5G 10 10 14.07 21.06 6.99 0.699 0.699H 10 10 13.39 19.86 6.47 0.647 0.647I 10 10 12.86 19.15 6.29 0.629 0.629

21.6%J 10 10 14.60 21.77 7.17 0.717

0.681 6.40.717

UK 10 9 14.08 20.02 5.94 0.660 0.59497.5 0.664 9.4

L 10 10 14.11 21,27 7.16 0.716 0,716M 10 10 14.30 21.08 6.78 0.678 0.678

43.2%N 10 10 14.69 21.43 6,74 0.674

0.667 2.50.674

5.70 10 10 14.77 21.19 6.42 0.642 0.642

100.0 0.667 2.5

P 10 10 12.48 19.22 6.74 0.674 0.67410 10 13.72 20.32 6.60 0.660 0.660

86.4%R 10 10 13.67 19.71 6.04 0.604

0.642 4.0 0.604100.0 0.642 4.0 9.2

5 10 10 13.07 19.64 6.57 0.657 0.657T 10 10 13.24 19.72 6.48 0.648 0.648U 10 10 13.23 19.44 6.21 0.621 0.621

100%V 10 10 14.98 21.32 6.34 0.634

0.631 6.3 0.63410.8

W 10 10 14.65 20.51 5.86 0.586 0.586100.0 ~6JI 6.3

X 10 10 13.58 20.40 6.82 0.682 0.682Y 10 10 14.01 21.35 7.34 0.734 0.734

100% IntakeZ 10 10 13.43 19.77 6.34 0.634

0.694 6.30.634

100.0 0.694 6.3 I.'AA 10 10 13.70 20.63 6.93 0.693 0.693BB 10 10 13.51 20.67 7.16 0.716 0.716

•••••~

Olltfall101:Dunnett's MSD value:PMSD:

0.06979.9

MSD=PMSD=

Minimum Significant DifferencePercent Minimum Significant Difference

PMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

Intake:Dunnett's MSD value:PMSD:

~0653

9.2Lower PMSD bound determined by USEPA (IOth percentile) = 12%.Upper PMSD bound determined by USEPA (90th pe£centile) = 30%.

Lower and upper PMSD bounds we£e determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 200 la; USEPA, 200 Ib).

USEPA. 200180 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-01-OO4 and EPA-821-B-01-OO5. US Environmental Protection Agency, Cincinnati, OR.

File: sqn101_050612dala.xlsxEntered by: J. s~rerReviewed by:~

of 110

i~TS,,:> .Snvl",nm.ntal r.,lIng Sal"IIO~',Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101Non-treated

May 08-15, 2012

Statistical Analyses

Larval Fish Growth and Survival Test-? Day GrowthTestlO: PpFRCR Sample ID: TVA! SQN, Outfall 101Lab 10: ETS-Envir. Testing Sol. Sample Type: DMR.Discharge Monitoring ReportProtocol: FWCHR-EPA-821-R-02-013 Test Species: PP·Pimephales promeJas

Start Date:End Date:Sample Date:Comments:

Conc-%

5/8/20125/15/2012May 2012Non-treated

1 2 3 4D-Control

10.821.643.286.4100

Intake

0.65800.62400.62900.67800.66000.62100.7340

0.68600.64800.71700.67400.60400.63400.6340

0.70700.69900.59400.64200.65700.58600.6930

0.77800.64700.71600.67400.64800.68200.7160

Transform: Untransformed 1-Talled IsotonicConc·% Mean N·Mean Mean Min Max CV% N t-Stat Critical MSO Mean N·Mean

D-Control 0.7073 1.0000 0.7073 0.8580 0.7780 7.248 4 0.7073 1.000010.8 0.6545 0.9254 0.6545 0.8240 0.6990 4.839 4 1.823 2.410 0.0697 0.6618 0.935821.6 0.6640 0.9388 0.6640 0.5940 0.7170 9.380 4 1.495 2.410 0.0697 0.6618 0.935843.2 0.6670 0.9431 0.6670 0.6420 0.6780 2.515 4 1.391 2.410 0.0697 0.6618 0.935886.4 0.6423 0.9081 0.6423 0.6040 0.6600 4.049 4 2.247 2.410 0.0697 0.6423 0.9081"100 0.6308 0.8918 0.6308 0.5860 0.6820 6.298 4 2.644 2.410 0.0697 0.6308 0.8918

Intake 0.6943 0,9816 0.6943 0.6340 0.7340 6.270 4

Auxiliary Tests Statistic Critical Skew KurtShaplro-Wllk's Test indicates normal distribution (p> 0.01) 0.97186 0.884 0.17128 ~0.4665

Bartlett's Test indicates equal varIances (p = 0.39) 5.23389 15.0863Hypothesis Test (1-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob dfDunnett's Test 86.4 100 92.9516 1.15741 0.06973 0.09859 0.00279 0.00167 0.19306 5,18Treatments vs D-Control

" indicates IG estimate less than the lowest concentration

15050 100

Dose %

1.0,------------,

0.90.8

0.7

31 0.6cg, 0.5

&0.4

0.3

0.2

0.1 l~:=;:::'~::::::::-+:~~J0.0o

Linear Interpolation (200 Resamples)95% CLfExp) SkewsoPoint %

IC05" 8.409IC10 93.170IC15 >100IC20 >100IG25 >100IC40 >100IG50 >100

Dose-Response Plot

T_~.L

0.9,-------------------,

0.8

0.7 r--- .T T......~ ,- - - - --J 0.6 ~

oI; 0.5

ia'0.4Q

.... 0.3

1~tall, 0.05 levelof significance

0.2

0.1

0g ~ ~ ~ ~ 0 J!0 N M • 0

" ~ • ,. 50 ~

"cl

Im'''F>I'nd."tRt\II""'b~

K'IIo~:....n File: sqnlO1_050812dala.:dsx

Entered by: J. S':ferReviewed by:~

of 110

••..•Start Date:End Date:Sample Date:Comments:

5/8/20125/15/2012~6}DNon-treated

TVA / Sequoyah Nuclear Plant, IntakeNon-treated

May 08-15, 2012


Larval Fish Growth and Survival Test-7 Day GrowthTest ID: PpFRCR Sample ID: TVA / SON, IntakeLab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring ReportProtocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas

Conc-% 1 2 3 4D-Control

10.821.643.286.4100

Intake

0.65800.62400.62900.67800.66000.62100.7340

0.68600.64800.71700.67400.60400.63400.6340

0.70700.69900.59400.6420,0.65700.58600.6930

0.77800.64700.71600.67400.64800.68200.7160

Transform: UntransformedConc-% Mean N-Mean Mean Min Max CV% N

D-Control 0.7073 1.0000 0.7073 0.6580 0.7780 7.248 410.8 0.6545 0.9254 0.6545 0.6240 0.6990 4.839 421.6 0.6640 0.9388 0.6640 0.5940 0.7170 9.380 443.2 0.6670 0.9431 0.6670 0.6420 0.6780 2.515 486.4 0.6423 0.9081 0.6423 0.6040 0.6600 4.049 4100 0.6308 0.8918 0.6308 0.5860 0.6820 6.298 4

Intake 0.6943 0.9816 0.6943 0.6340 0.7340 6.270 4

1-Tailedt-Stat Critical MSD

0.387 1.943 0.0653

CriticalAuxiliary TestsShapiro-Wilk's Test indicates normal distribution (p > 0.01)F-Test indicates egual variances (p = 0.79)Hypothesis Test (1-tail, 0.05)Homoscedastic t Test indicates no significant differencesTreatments vs D-Control

Statistic0.974621.38665MSDu MSDp

0.06534 0.09238

0.74947.4683

MSB0.00034

Skew Kurt0.1774 -0.6068

MSE F-Prob df0.00226 0.71238 1,6

Dose-Response Plot

0.9

0.8

0.7

lO.6e 0.5C)

~0.4Cto- 0.3

0.2

0.1

0 g co CC! N ..,. 0~0 M cD 0

c: ... c:;; ..,. co ... $0 .s;()

0

1-tail, 0.05 levelof significance

File: sqnlOl_050812data.xlsxEntered by: J. SumnerReviewed by:+

of 110

Page 5 of6


Analyst

100%

10.8%

21.6%

43.2%

86.4%

100%Intake

CONTROLNon-treated

Species: Pimephales promelas Date: _J.l",)(=,ollL'...I",--,==-__

Client: TVA / Seguoyah Nuclear Plant. Outfall 101. Nou-treatedDail Chemist : '

Concentration

I,

I

of 110

11

tj

Species: Pimeoha/es prome/asClient: TVA / Se uo ah Nuclear Plant Outfall 101 Non-treated

Concen- Parametertration

CONTROL P.'~~~---I-'--'--'---

Non-treated

10.8%

21.6%

43.2%

86.4%

100%

100%Intake

RoIli'WIly

K'II~...n

Page 6 of6

Da


of 110 7875Project Dumber:------

Daily Chemical Analyses

Pimephales promelas Chronic Whole Effluent Toxicity Test

EPA-821-R-02-013, Method 1000.0

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treatedMay 08-15, 2012

~-

Irim"~~i

~~TS~ Environmental Testing Solutions, Inc.

Concentration IParameter

25.01 24.81 24.617.62 8.101 7.497.0 7.61 6.4

291 -24.8 24.8 24.77.64 8.10 7.496.9 7.6 6.6

lI\ll!II 281

24.8 24.8 24.77.63 8.11 7.477.0 7.6 6.5... 263

25.1 24.8 24.7i7.65 8.12 7.466.9 7.6 6.~

218

24.88.i6

7.6199

8.02

DaInitial

Da

7.4i941l

7..98Initial

7.77.85

Final2

Final I Initial I Finalo I Davl

25.1

Total Residual Chlorine (m

DO (meiLConductivity (umbos/em'

Temperature ('C) I 24.9\ 24.6\ 25.01 24.7

'" (Sm I 7.911 7.901 8.011 7.82

!H(SU

Temperature (oC) I 24.91 24.61 25.01 24.8

Alkalinity (mg/L CaCO,)

DO m IL 7.81 7.4Conductivity (wnhoslcm) 297

'" (SU) I 7.891 7.861 7.961 7.78

'" (SUI I 7.921 7.901 8.021 7.86

Temperature ('C) I 25.01 24.61 25.01 24.6

DO m L '.7

Couductivi mhos/em •"..Alkalinity (mgIL CaC03)

Hardness (mgIL CaC03)

Total Residual Chlorine m

Temperature (0e)

DO (mg!L) I 791 791 781 7.6Conductivity (umhos/em) 291 Iii· -nti 284 !jjW:=§I"

Hardness (mgIL CaC03)

'" ISUI I 7.891 7.861 7.981 7.78

,Temoerature ('C)

'" ISU) I 7.891 7.901 7.991 7.82

'" Ism I 7.851 7.901 7.921 7.79

DO (mgIL) I 791 801 781 7.6Conduetivitv ClLIDhoslcml 2651!i!'": 71'; 259_I

Temperature r'C) I 24.91 24.91 25.01 24.7

Alkalinity (mg/L CaCOJ) 63 I u,)

Hardness (mgIL CaC03) 88

Temperature flC) 24.9

DO mConductivity (umbos/em)

no mConductivity (umhos/em)

100%

86.4%

43.2%

21.6%

10.8%

Control

100% Intake

File: sqn101_050812chem.xlsEntered by: C. Johnson

Reviewed by:-Jf--

of 110

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1002.0)Species: Ceriodaphnia dubia

Analyst

Page I of7

Connty: HamiltonOntfall: 101

Sample numbers usedOutfall 101 Intake

MHSWbatch used

Comments:10,8 21.6 432 86.4 ' 100

270 540 1080 2160 2500

2230 1960 1420 340 02500 2500 2500 ,2500 2500

Test initiation and feeding,renewal and feeding, or

termination time

Date

o

2

3

Daily renewal information:

4

Client: Tennessee Valley AuthorityFacility: Seguoyah Nuclear PlantNPDES #: TN0026450Project #: , &1$ ,

7

5

6

Day

Test orf!anism source information: Test information:Organism aile: < 24-hours old Randomizing temvlate color: &iI...t>Date and times organisms were born .0t.·08- \'1.;. 0..'\0 'll> O~· Incubator number and shelfbetween:

location: 1..c..\Culture board: OS-O\'\'1-

Replicate number: 1 I 2 1 3 41 5 1 6 1,71,8/9/'"YWT batch: t/S'O\o 1'1.;.Culture board cun number: 1ll 1'IJi hI. ~ I!ll. I\.; I~ I '!>\ I \10 !'I 0

Transfer vessel infonnation: I}H, • S.U. Temnerature t(.. \ °CSe/enastrum batch: (),\.~.\'L

Average t11U1sfer volume (mL): I (). ~'2.1-.l4

Control information: Summary oftest endpoints:Control-! Control-2 Accentance criteria

% ofMale Adults: M. 'r- 07. :520% 7-day LC,o ') IDD'1.% Adults having 3'" Broods: I 1n1. - . iiOf" >80% NOEC In"7.% Mortality: 101. • ::;;20% LOEC 'T 101\7.. •Mean OffsorinlZlFemale: 3\.''1 -!oo .r,. ~ 15-.0 offspring/female ChV '> 1()().1.%CV: lo :17. Io·n "<40.0% IC" >1007.

Ind""endentRo~.",by

"'~"" SOP AT II - Exhibit AT11.2, revision 06-01-11

of 110

Page 2 of7

Concentration:% Mortality: I 0".Mean OffsorilllzlFemale: I .'n.",

SOP ATlI - Exhibit ATlI.2, revision 06-01-11

Survival and ReproductIOn Data

Species: Ceriodaphnia dubiaClient: TVA I Segnoyah Nnelear Plant. OntfalllOI Date: OS. 08 .. 11-CONTROL-l SurVival and Reproduction Data

No/e. Adult mortality (L hve, D dead), 58 split brood (smgle brood spltt between two days), CO carry over (offspnng earned over WIth adult dunng transfer).

CONC' 108%

Replicate nnmberDav 1 2 3 4 5 6 7 8 9 10

1 Young produced () 0 0 0 0 0 0 a u (:)Adult mortality L L- L- L- I..- L- L l- L. L

2 Young produced j) D j) () j) 0 0 0 0 0Adult mortality '- L L- '- L L- L L \.- \.-

3 Young produced D D 0 0 C:J (\. C> 0 0 C>Adult mortality L \.- L- L- '- L. \... '- L L...

4 Young produced '"-\

'" ~ '-l l., '-l. c::. S '-I "-Adult mortality L... \..-. L. L... '-- L \..- '-- '-- L.

5 Young produced \'1_ \-:a.. \() n ,~ IL. 17., \"1_ l~ IhAdult mortality L \..- L. '-- L L- L L L '--

6 Voung produced (} D a 0 0 0 0 0 0 ()Adult mortality L L- L- L L. L L... L L L-

7 Young produced I' ,<- \e... \~ I\. ,'-I IS . \i \"'\ I""Total young produced

O~ 30'2. 3b ~I :!>Io. 31:> :!I!:l 3"\ 3\ '2.'\Final Adult Mortalitv L '- \... \..- '- '-- \..:..- \.... '- '--X for 3rd Broods )(. X. 'V' ')( X. >Co ~ ~ )C . '£

- -

.ReDlicate number

Day 1 2 3 4 5 ·6 7 8 9 10

1 ¥ol;lng produced () i\ f"\ (i 0 C C (j (') 1(")Adult mortality L L- L. '-- L L L l L '--

2 Young·produced (') 0 D 0 a 0 .tl C\ (') 0Adult mortality l... L L L l... L L L l... L..

3 Young produced 0 C> 0 (:) () 0 0 n tl 0Ad~lt mortality \..- L.. L- \... l- L L L L L-

4 Young produced 1.\ ~ '" S .3 \.\ \.\ '" '" .sAdult mortality L- L.. L L.. L.. '-- L.. '-- L L.

5 Young produced \1. \~ \1. \'1_ 1'2,) \ "'L 10 12.. I~ \ 1-Adult mortality L '- L L L L L L.. \... '--

6 Young produced () D 0 0 0 0 0 0 0 0Adult mortality L L.. L L L L.. L L L. L.

7 Young produced n \"" ,~ \10 \5 \\0, \'-\ 1\ 11 \STotal young produced 3>3 ~!:l 1>b !l~ .!l\ ~1. '22 as 1>S .!>1....Final Adult Mortality \. '" \... L '-- L L '- "- \..-.Note: Adult mortality (L live, D dead), 58 ~ split brood (single brood splil between two days), CO c over (off's rin clUried over with adult durin transfer).

Concentration:% Mortality: 0Mean Offspring/Female: . ,.,.~

% Reduction from Control-l: -0,,7.'"d.p,"~'"'Rovlowby

.,II·V£.K.",••

"L

II

I

-

IIIIIII

of 110

Page 3 of7

Survival and Reproduction Data

Species: Ceriodaphnia dubiaClient: TVA I Seguoyah Nuclear Plant, Outfall 101 Date: O$./lt"I'1_CONC' 21 6% Su,..,ival and Reproduction Data

°ETS•. ,~ '." I: '.:) \~'""<:: If 'h',,~'j~

~ EJWlnlnment,lTeSllngSeluUonr.-llI<.

CONC' 432%

., Renlicate number

Dav 1 2 3 " 5 6 7 8 9 101 Young produced a 0 0 a D 0 [) c, 0 IJ

Adult mortality \.... l- l- L- L- L- L.. L- L.- L..2 Young produced 0 0 0 0 0 0 0 0 0 0

Adult mortality L L- L l- L- L..- L. I- L L..3 Young produced (") 0 0 D 0 () (') 0 C\ ("}

Adult mortality L- L- \.... \.... L- \... L- L. \... L4 Young produced "'l '"'

\0 S S '-\ 5 .C;

'"'~

Adult mortality \.... "- '-- '- I- '- '- '-- l- I.....5 Young produced \~ \ "l... 1'--\ ~~ \\ \.", I'l... \2. \5, \'2_

Adult mortality \.... '- '- '- L \..... \..... L L '-6 Young produced a 0 0 a () 0 0 0 () (')

Adult mortality L L L. L. L L- \.... L- L L7 Young produced \"1 II.. ~\. IS Ie, '5 .1 Ii \So I'" \\0

Total young produced :!l\. :,'1. ~\" .;~ ~\ ~'2. ~S .:'>2. as ~

Final Adult Mortality "L L. '-- L- L. '- L. \,... \,... '-Note: Adull mortality (L ~ live, D dead), 58 = split brood (single brood split between two days), CO = c over am rin carried over with adult durin transfer).

Concentration:% Mortality: f'l1.Mean OffspringIFemale: a,.<..% Reduction from Control-I: • S.01.

.Replicate number

Dav 1 2 3 4 5 6 7 8 9 101 Young produced n (') () (j 0 () () 0 0 (')

Adult mortality L. L. L. L- L. L L L L. L.-2 Young produced .(") 0 0 n 0 (") () () () (')

Adult mortality L L. L. L L. L .L L L L.3 Young produced () () (') () D () 0 0 0 0

Adult mortality L L L L L... I- L L L L.....4 Young produced 4 '-I 4 \. '-l '-\ \0 -, So 5;

Adult mortality \.... \.... L... L L. L..... '- '- L. '-5 Young produced 1Y. L1... \4 \'-\ \""'L l'l..... 1\ \~ I"'L \~

J\dult mortality. \.... L.. \.... \..... \..... L- L L... '- '-6 Young produced 1"\ ('") () 0 0 0 0 U u (")

Adult mortality L L L L L. L L L L L..7 Young produced \'1. \<; \S \"\ n \ ,,\ 1& \'\ 1\0. \'5

Total young produced!>S "3\ .3~ 31 3.3 3b .35 31 ..3.3 33

Final Adult Mortality L... L.. '-- '- '- '- '- '- L.. '--Note: Adult mortality {L = live, D dead),58 split brood (single brood split between two days), CO ca over offs rin carned over with adult durin transfer).

Concentration:% Mortality: 07.Mean OffspringIFemale: ~.C;% Reduction from Control-I: -(,,37..

l

In;epend,ntR,lIIewlly

t<eIIOy!.Keo..n

~ SOP ATI I - Exhibit ATI 1.2, revision 06-0 I-II

of 110

Page 4 of7


Species: Ceriodaphnia dubiaClient: TVA I Seguoyah Nnclear Plant. Outfall 101 Date: 0$.0'-11..CONC' 864% Survival and Reproduction Data

CONC' 100%RenJicate number

Day 1 2 3 4 5 6 7 8 9 101 Young produced (') () f\ t\ f\ (j (') 11 0 0

Adult mortality L \.. L L L. L L L L. L2 Young produced 0 D () 0 () (") 0 0 (j 0

Adult mortality L L L '- L. '- L... L L L3 Young produced () 0 D f) (') r) 0 0 0 (')

Adult mortality L L L L L.. \.- L L ~ L..4 Young produced ~ .<; "" '4 '-\ ~ 'E:. l." ~ S

Adult mort~lity \... '-- L L... L L... '-- '-- L \-..5 Young produced '0 \'2... '"b \ .... I~ ,<; ,~ \"L ,~ l"'l

Adult mortality ~ L. L L L. L.. L L L L6 Young produced 0 0 0 0 (\ 0 0 a u (J

Adult mortality L L L L. \.... L '- L "L.. L.7 Young produced \8 L"l II.> I" 20 I~ \5 Ig 11

"Total young produced31. 'SI. ?IS as 31 3~ ~ ~c.. ?IS 310

Final Adult Mortality L \.... '- L L. L .,- L \.... LNote: Adult monality (L live, D ~ dead), S8 split brood (single brood split between two days), CO over offs rin carried over with adult durin transfer).

Concentration:% Mortality: 01Mean Offspring/Female: .~C:. p% Reduction from Control-I: ~I2.17.

.ReDlicate number

Dav 1 2 3 4 5 6 7 8 9 101 Young produced 0 a (") (") 0 0 () Cl 0 0

Adult mortality l L L. L. L. L- L L- L L..

2 Young produced 0 f) 0 () r, () () 0 0 0Adult mortality L L L.. L. L L. L.. L.. L L..

3 Young produced (") () 0 0 D 0 0 0 0 C\Adult mortality L L. L- L- l.- . \.-. '- '-- L L-

4 Young produced c:.. <:. '-l S '" \0 <4 ~ '-\ \0Adult mortality L.. L '- '- '- '- '- L.. '- L-

.

\')., \b 'S \b \~ \ 'L5 Young produced l"L. , t \, 17-. Adult mortality L '- L... L '- L L... L L. L

6 Young produced 0 D ,...." () () a b 0 0 0Adult mortality L L. \. L L. L. L. L L L

7 Young produced I' \& IS \~ 1'\ I'" 1'\ \1 n 11Total young produced .!I'-l 31. 3'1. 35 31 35 3'-\ ~'-\ ~~ 35Final Adult Mortality \. L '- '- \.... ' . "- \... '-- '- L..Note: Adult monality. (L '" live, D dead), S8 = split brood (single brood split between two days), CO "Cllf' over (offsprin~ carried over' with adult durin transfer).

Concentration:% Mortality: N.Mean Offspring/Female: :!I't.S% Reduction from ControJ·I: -g. '2.7.

j!'c,:;'cee'

-'l

Ind.".n.'"Re\1ewb~

K.II'VE.K,.nanInhf SOP ATil - Exhibit ATl1.2, revision 06-0 I-II

of 110

Page 5 of7

Concentration:% Mortality: I D1.Mean Offspring/Female: I •':f,O .10

SOP ATil - ExhIbIt ATl1.2, reVISIOn 06·01 -11


Species: Ceriodaphnia dubiaClient: TVA I Seguoyah Nuclear Plant, Outfall 101 Date:CONTROL-2 Survival and Reproduction Data

Nate. Adult mortality (L hve, D dead). S8 split brood (smgle brood spilt between two days), CO carry over (offspnng earned over WIth adult dunng transfer).

CONC' 100% Intake

Replicate number

Dav 1 2 3 4 5 6 7 8 9 101 Young produced (J () (J (J C) (') (') n () ()

Adult mortality '-- 1 \...... L \...... L L. L .L- L..2 Young produced 0 0 0 0 0 0 0 0 0 ()

Adult mortality "- \...... \...... L \...... L. L L L- L:-3 Young produced ("") 0 0 r-, () 0 0 Cl 0 n

Adult mortality L \..- L.. L '- L. '- L.... L '--4 Young produced '-l \.. S 4 4 ,<:; "-I. '-\ '-I 3-

Adult mortality '- '- \....... I- L L L L L "~

5 Young produced \{:) I~ \"'L 11. \.7\ 1 \ \ \ 1\ \ \ IbAdult mortality \.... '- '-- L L. '- \. \... L '--

6 Young produced 'f") (', 0 0 0 (:; 0 0 () 0Adult mortality '- \.... L L L- L. '- L L.. L

7 Young produced \10 \L.\ ~"" 14 1'-\ l~ ~'l 11. 11. \~Total young produced

30 3~ 3~ 3b 3\ '2.'; 32- .3\ 31 2.r..Final Adult Mortalitv L L '- L.. '- L.. l \.... L \.X for 3 r

(l Broods )C.... X ~ x. .x... '7'- ;;c... )C... X )<.

Replicate numberDay 1 2 3 4 5 6 7 8 9 10

1 Young produced n f\ r. n n (') (\ () (') 0Adult mortality L L L 1 1 L L L L l-

2 Young produced () () n 0 0 (J .() 0 a 0Adult mortality L L L L L. L. L L L L

3 Young produced (j 0 0 0 0 (J 0 () 0 ()

Adult mortality L L L L L L L L L.. L.4 Young produced '"\ ..... S '-\ .s \" (., lo '-l S

Adul~ mortality '- L L.. \.... '-- L.. l- I.... I.,- 'L..5 Young produced \b \~ 10 1'-\ I':!:. 1"2... I~ ID n .. I \

Adult mortality L '- '-- L '- L L.. L.. L.. '-6 Young produced (') 0 () () 0 0 0 () 0 0

Adult mortality L L L- '- L L L L L L.7 Young produced II. I' 14 \4 I~ \l2 \3 \S I' \5

Total young produced 33. as 2"1 3"t 3\ 31. ..3'2. 3\ 3~ ..3\Final Adult Mortality \.. L L '-- L \... ~ L '- '"--Note: Adult mortality (L live, 0 dead). SB split brood (single brood split between two days), CO over off!; rin carried over with adult durin transfer.

Concentration:% Mortality: 07.Mean OffspringIFemale: 3t·'!.% Reduction from ControI~2: -S.r..7-

. . ..

,"d.pen~.M

RovIowby

I<elloyE,~"""

I~

~IIIIIII

of 110

I'SlH, ~;!l

~ETS~ Envlronm..ntal TesUng SolutIons, Inc.

TVA I Sequoyah Nuclear Plant, Outfall 101 - Non-treatedMay 08-15, 2012

Verification of Ceriodaphnia Reproduction Totals

86.4%- -~

Day Re Iicate numberTotal

I 2 3 4 5 • 7 8 9 101 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 0

• 5 5 4 5 5 6 • 4 4 6 485 12 13 13 IS 13 13 11 13 12 12 127

• 0 0 0 0 0 0 0 0 0 0 07 17 18 IS IS 19 16 19 17 17 17 170

Total 34 3. 32 35 37 35 34 34 33 35 345

Control-l

DayRe Iicate number Total

I 2 3 4 5 • 7 8 9 10

I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 4 5 4 • 4 5 5 4 5 4.5 12 13 10 12 14 12 13 12 13 10 121

• 0 0 0 0 0 0 0 0 0 0 07 17 IS IS IS 16 14 IS 17 14 14 152

Total 33 32 30 31 3. 30 33 34 31 29 319

100%-- - .-

Day Re licate DumberTotal

I 2 3 4 5 • 7 8 9 10

1 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 6 5 6 4 4 5 5 6 4 5 505 13 12 13 12 13 IS 13 12 14 14 1316 0 0 0 0 0 0 0 0 0 0 07 18 19 16 19 20 18 IS 18 17 17 177

Total 37 36 35 35 37 38 33 36 35 3. 358

10.8%-_.- --

Day Re licate DumberTotal

1 2 3 4 5 • 7 8 9 10

1 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 4 5 5 3 4 4 6 5 5 455 12 13 12 12 13 12 10 12 13 12 1216 0 0 0 0 0 0 0 0 0 0 07 17 16 13 16 IS 16 14 17 17 IS 1S6

Total 33 33 30 33 31 32 28 35 35 32 322

Coutrol-2

Day Re Iicate numberTotal

1 2 3 • 5 • 7 8 9 10I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 6 5 4 4 5 • • 4 3 435 10 13 12 12 13 11 11 11 11 10 11.6 0 0 0 0 0 0 0 0 0 0 07 16 14 16 14 14 13 17 16 16 13 149

Total 30 33 33 30 31 29 32 31 31 26 306

21.6%-~.- --

DayRe licate number

Total1 2 3 4 5 6 7 8 9 10

I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 4 6 5 5 4 5 5 4 5 475 13 12 14 13 11 13 12 12 IS 12 127

• 0 0 0 0 0 0 0 0 0 0 07 19 16 16 IS IS IS 18 IS 16 16 161

Total 36 32 36 33 31 32 35 32 35 33 335

100% Iutake

Day Re licate numberTotal

1 2 3 4 5 • 7 8 9 10I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 4 5 4 5 6 6 6 4 5 495 13 14 10 14 13 12 13 10 12 11 1226 0 0 0 0 0 0 0 0 0 0 07 16 17 14 14 13 18 13 IS 17 IS 1S2

Total 33 35 29 32 31 36 32 31 33 31 323

43.2%._.- --Day

Re licate numberTotal

1 2 3 4 5 6 7 8 9 10

1 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 4 4 6 4 4 6 5 5 5 475 14 12 14 14 12 12 11 13 12 13 127

• 0 0 0 0 0 0 0 0 0 0 07 17 IS IS 19 17 14 18 19 16 IS 165

Total 35 31 33 39 33 30 35 37 33 33 339

File: sqn101_050812data.xlsxEntered by: J. s~nerReviewed by:~

of 110

!=.. T..r.... 1~'.·:.:\~ ,> ~

I~H{~f}

••CD@

o."~ Environmental Testing Solutions, Inc.

TVA / Sequoyah Nuclear Plant, Outfall 101Non-treated

May 08-15, 2012

Ceriodaphnia dubia Chronic Whole Efflnent Toxicity TestEPA-821-R-02-013, Method 1002.0

Qnality ControlVerification of Data Entry, Calcnlations, and Statistical Analyses

7875Project number' ,~.~

Concentration Reolicate number Survival Average reproduction Coefficient of Percent reduction from

(%) (Ufo) (offspring/female) variation ('Yo) control (%)1 2 3 4 5 6 7 8 9 10

Control-I 33 32 30 31 36 30 33 34 31 29 100 31.9 6.7 Not applicable

10.8% 33 33 30 33 31 32 28 35 35 32 100 32.2 6.7 -0.9

21.6% 36 32 36 33 31 32 35 32 35 33 100 33.5 5.5 -5.0

43.2% 35 31 33 39 33 30 35 37 33 33 100 33.9 7.9 -6.3

86.4% 34 36 32 35 37 35 34 34 33 35 100 34.5 4.2 -8.2

100% 37 36 35 35 37 38 33 36 35 36 100 35.8 3.9 -12.2

Control-2 30 33 33 30 31 29 32 31 31 26 100 30.6 6.8 Not applicable

100% Intake 33 35 29 32 31 36 32 31 33 31 100 32.3 6.4 -5.6

Outfall 101:Dunnett's MSD value:PMSD:

Intake:

Dunnett's MSD value:

PMSD:

2.0366.4

1.5995.2

MSD~

PMSD=Minimum Significant DifferencePercent Minimum Significant DifferencePMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statisticallysignificant in a whole effluent toxicity test.

Lower PMSD bound detennined by USEPA (lOlh percentile) = 13%.

Upper PMSD bound detennined by USEPA (90th percentile) = 47%.Lower and upper PMSD bounds were detennined from the 10th and 90th percentile, respectively, ofPMSD data from EPA's WET InterlaboratoryVariability Study (USEPA, 2001a; USEPA, 200Ib).

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-Q0-Q03. US Environmental ProtectionAgency, Cincinnati, OR.

USEPA. 2001a, 2001 b. Final Report: Interlaboratory Variability Study of EPA Short~tenn Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes I and 2-Appendix. EPA~821-B-OI-004 and EPA-821-B-Ol-005.US Environmental Protection Agency, Cincinnati, OH.

File: sqn101_050812data.xlsxTable populated from associated ''Verification of Ceriodaphnia Reproduction Totals" spreadsheet.

Spreadsheet entered by: J. SumnerReviewed by: -0\---

of 110

~~T£~ EnvlronmonlBlTosIl"!I 501"'lon.. Inc.

TVA I Sequoyah Nuclear Plant, Outfall 101Non-treated

May 08-15, 2012


Cerlodaphnia Survival and Reproduction Test-ReproductionStart Date: 5/8/2012 Test ID: CdFRCR Sample 10: TVA/SON 101End Date: 5/15/2012 Lab ID: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring ReportSample Date: May 2012 Protocol: FWCHR-EPA-821-R-02·013 Test Species: CD-Ceriodaphnia dubiaComments:

Conc-% 1 2 3 4 5 6 7 8 9 1aControl~1 33.000 32.000 30.000 31.000 36.000 30.000 33.000 34.000 31.000 29.000Control~2 30.000 33.000 33.000 30.000 31.000 29.000 32.000 31.000 31.000 26.000

10.8 33.000 33.000 30.000 33.000 31.000 32.000 28.000 35.000 35.000 32.00021.6 36.000 32.000 36.000 33.000 31.000 32.000 35.000 32.000 35.000 33.00043.2 35.000 31.000 33.000 39.000 33.000 30.000 35.000 37.000 33.000 33.00086.4 34.000 36.000 32.000 35.000 37.000 35.000 34.000 34.000 33.000 35.000laa 37.000 36.000 35.000 35.000 37.000 38.000 33.000 36.000 35.000 36.000

Intake 33.000 35.000 29.000 32.000 31.000 36.000 32.000 31.000 33.000 31.000

Transform: Untransformed i-Tailed IsotonicConc-% Mean N·Mean Mean Min Max CV% N t·Stat Critical MSD Mean N-Mean

Control-1 31.900 1.0425 31.900 29.000 36.000 6.683 la 33.633 1.0000Control-2 30.600 1.0000 30.600 26.000 33.000 6.750 la

10.8 32.200 1.0523 32.200 28.000 35.000 6.677 la -0.337 2.287 2.036 33.633 1.000021.6 33.500 1.0948 33.500 31.000 36.000 5.495 1a -1.797 2.287 2.036 33.633 1.000043.2 33.900 1.1078 33.900 30.000 39.000 7.921 la -2.246 2.287 2.036 33.633 1.000086.4 34.500 1.1275 34.500 32.000 37.000 4.156 la ~2.920 2.287 2.036 33.633 1.0000100 35.800 1.1699 35.800 33.000 38.000 3.906 10 -4.381 2.287 2.036 33.633 1.0000

Intake 32.300 1.0556 32.300 29.000 36.000 6.370 10

Auxiliary Tests Statistic Critical Skew KurtKolmogorov D Test indicates normal distribution (p > 0.01) 0.68163 1.035 0.20651 0.0673Bartlett's Test indicates equal variances (p = 0.37) 5.43364 15.0863The control means are not significantly different (p = 0.18) 1.38493 2.10092Hypothesis Test (i-tail, 0.05) NOEC LOEC ChV TU MSDu MSDp MSB MSE F-Prob dfDunnett's Test 100 >100 1 2.03577 0.06382 21.1867 3.96296 4.6E-04 5,54Treatments vs Control~1

Point % SOLinear Interpolation (200 Resamples)

95% CL Skew

15050 100Dose %

1.0.,------------,

0.9

0.8

0.7

0.6

~ 0.5&. 0.4

~ 0.3

a.'0.1

0.0 f-o-;+---'<>---+..... --... --0.1-a.' -h_~~~_~_~--.-I

a

>100>100>100>100>100>100>100

IC05rC10IC15IC20IC25IC40IC50

Dose-Response Plot

45

40

35

0 30

~ 25

"i 20

II: 15

10

5

0N

~~ .. .. a "£ ~ N w..

< <0 0

" "

1-tall, 0.05 levelof signlflcance

is

Ind.p.nd.n'R,vi'wby

K.II..,E.K....n

~"File: sqnlOl_050812dataxlsx

Entered by: J. StulUler

ReviewedbY:~

of 110

eElS••o "" ,;"";,,o '" ,",',~ EnvIronmental Testing Solutions, rnc.

TVA / Sequoyah Nuclear Plant, IntakeNon-treated

May 08-15, 2012


Ceriodaphnia Survival and Reproduction Test-ReproductionStart Date: 51812012 Test 10: CdFRCR Sample 10: TVA 1SON 101 - IntakeEnd Date: 511512012 Lab 10: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring ReportSample Date: May 2012 Protocol: FWCHR-EPA-821-R-02-013 Test Species: CD-Ceriodaphnia dubiaComments:

Conc-% 1 2 3 4 5 6 7 8 9 10Control-1 33.000 32.000 30.000 31.000 36.000 30.000 33.000 34.000 31.000 29.000Control-2 30.000 33.000 33.000 30.000 31.000 29.000 32.000 31.000 31.000 26.000

10.8 33.000 33.000 30.000 33.000 31.000 32.000 28.000 35.000 35.000 32.00021.6 36.000 32.000 36.000 33.000 31.000 32.000 35.000 32.000 35.000 33.00043.2 35.000 31.000 33.000 39.000 33.000 30.000 35.000 37.000 33.000 33.00086.4 34.000 36.000 32.000 35.000 37.000 35.000 34.000 34.000 33.000 35.000100 37.000 36.000 35.000 35.000 37.000 38.000 33.000 36.000 35.000 36.000

Intake 33.000 35.000 29.000 32.000 31.000 36.000 32.000 31.000 33.000 31.000

Transform: Untransformed i-TailedConc-% Mean NwMean Mean Min Max CV% N t-Stat Critical MSD

Control-1 31.900 1.0425 31.900 29.000 36.000 6.683 10Control-2 30.600 1.0000 30.600 26.000 33.000 6.750 10

10.8 32.200 1.0523 32.200 28.000 35.000 6.677 1021.6 33.500 1.0948 33.500 31.000 36.000 5.495 1043.2 33.900 1.1078 33.900 30.000 39.000 7.921 1086.4 34.500 1.1275 34.500 32.000 37.000 4.156 10100 35.800 1.1699 35.800 33.000 38.000 3.906 10

Intake 32.300 1.0556 32.300 29.000 36.000 6.370 10 -1.844 1.734 1.599

Auxiliary Tests Statistic Critical Skew KurtShapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.97072 0.868 -0.3101 0.44033F-Test indicates equal variances (p = 0.99) 1.00787 6.54109The control means are not significantly different Cp = 0.18) 1.38493 2.10092Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob dfHomoscedastic t Test indicates no significant differences 1.59873 0.05225 14.45 4.25 0.08172 1, 18Treatments vs Control-2

Dose-Response Plot

45,---------------------,

40

35

10

5

T T-.IT T.L .L 1----...,

----1- ----1- ---------------------------- 1-tail, 0.05 levelof significance

In,,"pend,nl

~-.

~'I"~"""

0

g ~.,

"' '" " 0 iig 0 OJ '" '"0

~ ... '"~ l'l

c c ..8 0

"

File: sqn101_050812data.xlsxEntered by: 1. S~erReviewed by:~

of 110

Species: Ceriodophnio dubioClient: TyA I Segunyah Nuclear Plant. Outfall 101

Doil Chemist :

Page 6 of7

Date: OS-O' '1]..

SOP ATIl - Exhibit ATI1.2, revision 06-01-11

11

&\

Initia~

43.2%

21.6%

Anal st

100%

10.8%

86.4%

100%Intake

CONTROL

Concentration Parameter

Indep<ndO<1l"vi,wb,

K.IIII'/~.K••",,"

I~

of 110

I"',"

."th":.

i·e.

IIIIIII

IIIIIIIII!I

Species: Ceriodaphnia dubiaClient: TVA / Seguoyah Nuclear Plant. Outfall 101


CONTROL

10.8%

21.6%

43.2%

86.4%

100%

100%lutake

Page 7 of7

Date: 0$.01-11...

SOP AT! 1- Exhibit AT! 1.2, revision 06-01-11

of 110

7875Project Dumber:-----'-'--'-


Ceriodaphnia dubia Chronic Whole Effluent Toxicity Test

EPA-821-R-02-013, Method 1002.0

TVA / Sequoyah Nuclear Plant, Outfall 101 - Non-treatedMay 08-15, 2012

~.-

~Jm,

eElS••+ - ",,-' ,-.~.~

~ '"ct. .. !1i" ..• . . /"\'

() Environmental Testing Solutions, Inc.

8.07.85

8.07.8625.0

24.9

7.72581's,.

7.7212l.i'

7.7280lfi

24.88.10

8.09

Dav3

7.72941",:

8.10

~76

1 ~294~~j·.. JV7 ;63 ";"',",',',- ,.....,......- -- "

8.07

24.8

24.8

...E.24.8

Initial Final7.98 8.02

1 T Da 2Final I Initial Final

7.961 7.95 8.0iT7.7 7.81303

63Wm n

89liiliiif11 1M'25.0 25.1 24.9 24.97.96 7.97 7.90 8.01

7.8 7.6 7.8 7.8297

. 292 , i25.2 24.8 25.0 24.97.98 7.97 7.92 8.01

7.8 7.6 7.8 7.8284 ----. 280"'ill25.2 25.1 25.0 24.917.99 7.97 7.91 8.02

7.8 7.6 7.8 7.81259_I. 2577 i I25.2 25.2 25.0 25.08.01 8.01 7.88 8.037.9 7.6 7.9 7.8

210 lIJlJ i1L!ilt 211 iIII --

25.2 25.0 25.08.04 7.89 8.06

7.9 7.8197

",-,~'==-",,=

7.998.0

8.1

25.2

7.95252

DO(m~

DO(mgl )Conductivity (J,UIlhoslcm

Conductivity (lUDboslcm

Temperature f'C)

U(s·

H(SU

IRes

H(SU

Temperature (Oq

Conductivity (umhos/em'

DOlmgJ ,Conductivity (umbos/em

Alkalinity (mglL CaC03)

Temperature (0e)

DO (mglLlConductivity (Jlmhos/em

oR(S

Hardness em-gIL CaC03)

Total Residual Chlorine (m~

iH (SU

DO (mglL)Conductivity (umbos/em

Temoerature (0C)

Temperature ('C)

Hardness (mgIL CaCOJ )

Conductivity (llIDhoslcm

!DOCm:

ITemperature ('I

~AlkaliDity (mgIL CaC03)

21.6%

100%

86.4%

43.2%

10.8%

Control

DO(m:H(SU

Temperature (Oq

Total Residual Chlorine (ml!!L'

100% Intake IAlkalinity (mgIL CaC03) I //Hardness (mglL CaCO,) --

Concentration IParameter

File: sqn101_050812chem.xlsEntered by: C. JohnsonReviewed by:-a\-

of 110

Dilution preparation information: Comments:Dilution prep (%) 10.8 21.6 43.2 86.4 100 Elich concentration was UV-treatedEffluent volume (mL) 270 540 1080 2160 2500 for 2 minutes to remove pathogenicDiluent volume (mL) 2230 1960 1420 340 0 Interferences.Total volume (mL) 2500 2500 2500 2500 2500

Test orl!anism information: Test information:Omanism a~e: 1'1.'1 .\, .,n<- l\~ Randomizing template: -e.L\)-LDate and times organisms o~o 0"\0 n. \I0I:>O Incubator number and .?>e.were born between: shelf location:Organism source: ~~ ~,t.~\ (>f OS-Cf\-rL Artemia CHM number: ell'" "c;"z.

Drying information for weightdetermination:

Transfer bowl information: pH= '.1"1 S.U. Date / Time in oven: ..... 1... I'\..Temoerature = "L'\. f/ 'c Initial oyen temoerature: 101'\·C.

Average transfer volume:O. \1..0'l-Jl

Date / Time out of oven: IM-.IL·\'\.. IFinal oven temperature: bo'tTotal drying time: l.",.I\oolt~

County: RheaOutfall: 101

Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013 Method 1000.0)Species: Pimephales promelas

Client: Tennessee Valley AuthorityFacility: Seguoyah Nuclear PlantNPDES #: TN0020168Project #: __' ...&...1....5........ _


Page 10f6

\'10

MHSWbatchused

•

Sample numbers used


Test initiation,renewal, ortermination

Acceptance criteria

61.

Afternoon feedingMorning feedingDate

Control in ormation:% MorlaH :Average weight per initial larvae:Average weight per surviving larvae:

Time Anal st Time Anal st Time Anal st Outfall 101 Intake0 IS'-lS

1'\002

14~3 1"'004 11.\005 .'1006 l"leo7

Independe"'1Ie";,wby

KoIIO'/E.K..nan

'VL

Day

~•

~!

~III

of 110

,..."".......l\o~owbV

••n.V!.•'·""·

lie SOP Ar20 - Exhibit Ar20.3, revision 06·01·11

Day CONTROL 10.8% 21.6%

A B C D E F G· H I J K L

0 10 10 /0 10 10 '/0 '. /0 10 10 I(J '0101

It> It> I(J 10 It> 10 10 10 10 10 10102

10 ID 10 10 ID 10 It;) 10 10 10 I() 103

10 /0 10 10 10 It> 10 10 10 10 1O104

10 10 Ie 10 10 /0 10 10 10 10/0 105

Ie 10 10 /0 10 ./0 10 10 10 10 10 to6

/Q It> 10 to It;) 10 10 10 10 10 10107

tt',) It> /0 /0 10 10 IC Ie 10 10 10 IQA - Pan weight (mg)Tray color code:: ~Hw...

13.H I!.o;t. 14-.I~ I';.t; 14-.3~ ''5.'14 1'5.8'\ I~.~f 14.1'1. r!>...4 1~.1'J I+.or.Analyst:Date: DS'QlAY

B - Pan +Larv~ht (mg)

. 14.118 20,03 ,'t..~O 1't:Z."f 1J),~ 20.15 2.0.115 Zo.'Iz. 14.16 ,q.llp ,,,,z.1.J 20,111Analyst:Date: ~I:utC - Larvae weight (mg) - B - A

Hand calculated. AI, ~.'\\ Co,"''' s.a1 ~,~I\ Co.~1. ...'2:0 ....\10 Io,lta s.~ fo .11. !",SCI l..~

Analyst: v -Weight per initial number of larvae (mg)= C I Initial number of larvae 0-. rv' ~ 4' to' ~o-.. Il,

~ ;f:' ,J' .0\, . Sf ~ i:!Hand calculated•. .)' ~ ... '0 \0 \0 " c.; " '"" \y O' (rAnalyst: . '" - O· 0' O· O' O' 0' I)' \)"O'Average weight per Percent reduction

O.l.t.\S - I{J.l7, . O.l.'2.~ - '" ,'S. 7.initial number of from control (%) O. 'S&Colarvae (mo)


Page 2 of6

Date: oS ",8' \ 1-Species: Pimephales promelasClient: TVA / Seguoyah Nuclear Plant, Outfall 101, UV-treated

Comments:

Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk =sick, sm =unusually small,19 = unusually large, d&r = decanted and returned, w =wounded.

~ EnvllllnmentlllTesting Solutions, Inc.

~IIIII

of 110

iET~ EnvfronmentalTestJng Salutions,.Jnc.

Species: Pimephales promelasClient: TVA I Segnoyah Nuclear Plant, Out(alll0l, UV-treated Date: oS'O~, 11,...

Page 3 of6

Survival and Growth DataDay 43.2% 86.4% 100%

M N 0 p Q R S T U V W X

0 ID ID 10 10 10 It; /0 to /0 10 /0 /01 10 10 /0 10 10 10 10 /0 10 1010 102

10 10 It> It> Ie 10 to 10 1O 10 ID 103

/0 /0 /0 10 10 fO 10 fO fO fO 10 104

/0 10 10 10 10 /0 (t> ID 10 (0 10I'D5

/0 10 Ie I'D ID 10 10 /0 10 to to /06 to 10 /0 10 to (0 10 (0 10 ID 10 107

tD 10 /0 /0 I(J 10 10 IQ 10 10It) JDA = Pan weight (mg)Tray color code:: ~

14.41.\'3.'\3 1!l.St 14:~'5 1!l.tI. IMo \'3.'31. 14.~\ I3·SIl 14.42- 14.0:} 1'3.I~Analyst: MDate: °5·ot.·\1..

B ~ Pan + Larv:r{ (lOg)

1'1.59 1'1.111 21.2.tt /'I.5Z l'l.~ Zo.lCil 1'I,lflt 1'1.51 2,O,Sl %.1.22- 20.03 j'l.1,5Analyst: ~Date: OJ.I~

C - Larvae weight (mg) - B - A

Hand calculated. J _ S.t.'i 10.0'\ ,,:!><.\ 10.010 5.!>1 S·1!> 10.10 5.100 (,.1!l ",ISO 10.1<" "',S!>Analyst: 0 ...Weight per initial number of larvae (mg)= C I Initial number of larvae

",:>" "," ~:.., ~\) 'DO ,,"":J .J.O ~ ~'lDt.., ~ :t ~Hand calculated•. 01 ~ '16 '" '16 ~ \0 O' ... '" \0

O' O' O' 'i:i ()' \l' b' \). o· c' O'Analyst: ..

Average weight per Percent reductionO.t..O'-l 2..17. 0.1011 ·1"'.~ltinitial number of from control (%) -3.01. O.S1b

larvae (100)

Comment codes: c = clear. d = dead, fg =fungus. k =kiIled, m =missing, sk = sick, sm ~ unusually small,Ig ~ unusually large, d&r ~ decanted and returned, w =wounded.

Comments:


of 110

Species: Pimephales promelasClient: TVA I Seguoyah Nuclear Plant, OutCa11101, UV-treated

Page 4 of6

Date:

Survival and Growth Data100% Intake

y Z AA BB

ID 10 to10 10 10

/0 /0 to10 q,Q. ID 10

Ie "\ (0 10

10 &\ LO 10

IU q 10 10"It

II) ~ 10 (0

13.t , 13.8; IS.7.,\

IQ,14l IQ.v.i 20,1'0

S!l1 s.'\'l. S,\'\

"- o-'V "- ~

tt t? ~ ~0' I)' o' Q"

0,(,,1"1 - '"\.17.

o

Day

6

C = Larvae weight (mg) = B - A

A = Pan weight (mg)Tray color code:: t1\~~'a..Analyst: I't\~

Date: os..ot.· \1.-

7

2

1

4

B = Pan +Lar~eweOght (mg)Analyst: _

Dale: 06.1:

Hand calCulatedyAnalyst: C3-l -

3

5

Average weight per Percent reductioninitial number of from control (%)larvae em )

Weight per initial number oflarvae (mg)= C I,Initial number of larvae

Hand calculated. yAnalyst: e

I

Comment codes: c ~ clear, d ~ dead, fg ~ fungus, k ~ killed, m =missing, sk ~ sick, sm ~ unusually small,19 ~ unusually large, d&r = decanted and retumed, w ~ wounded.

Comments:

Inaeperl<!e"'Rel'i,w~y

K.IIO'/E.~ ..na"

1A.:1S: SOP AT20 - Exhibit AT20.3, revision 06-01-11

of 110

I~!!\ ,;gj

TVA I Sequoyah Nuclear Plant, Outfall 101UV-treated

May 08-15, 2012

7875

Pimephales promelas Chronic Whole Effluent Toxicity TestEPA-821-R-02-013, Method 1000.0


Project Dumber.Environmental Testing Solutions, IncNot for Com Hance Assessment, Internal Laborato C

Coru:nl;ration (%) Replicllfe lDitlalllllmber of Final umber 0I1a..."" A _ Paa ....ight (Ing) B '"' Pan +Larvae Un"" ....;gllt (mg) Welght'Surviving Meall...eillbtl CoetIIclent arvlUiatkm Welghtllnltialnlllaber MoaD.urvl.vai Me.... weight IlDltlai Coelficj.,ntof p.,,,,enf ",dqotioD from

IlIrvae welght(1ng) '"'A_B nllmbe:r orlanae (mg) SnrviriDg number of (Meo.W<lRht,... ............ ofla...... (mg) (%) IInmbe:roflarvae variation (Moan wrigh! cotltrol (%)

larvae (mg) ..........n......1W.) ("'" 1""lniljaln::orluno)

A 10 (Q 13.77 19.68 5.91 0.591 0.591

ControlB 10 (Q 13.56 20.03 6.47 0.647

0.586 9.90.647

100.0 0.586 ••• Not applicableC (Q (Q 14.13 19.20 5.Q7 0.507 0.507D 10 (Q 13.25 19.24 5.99 0.599 0.599

E 10 10 14.39 20.85 6.46 0.646 0.646

10.8%F 10 10 13.94 20.15 6.21 0.621

0.645 5.8 0.621100.0 0.645 5.8 -10.1

G 10 10 13.89 20.05 6.16 0.616 0.616H 10 10 13.94 20.92 6.98 0.698 0.698

I 10 10 14.12 19.65 5.53 0.553 0.553

21.6%J 10 10 13.54 19.76 6.22 0.622

0.624 8.20.622

100.0 0.624 8.2 ".SK (Q 10 12.70 19.29 6.59 0.659 0.659L 10 10 14.06 20.69 6.63 0.663 0.663M 10 (Q 13.93 19.58 5.65 0.565 0.565

43.2%N 10 (Q 13.52 19.61 6,09 0.609

0.604 4.7 0.609100.0 0.604 4.7 -3.0

0 10 (Q 14.95 21.29 6.34 0.634 0.634P 10 (Q 13.26 19.32 6.06 0.606 0.606

10 10 14.40 19.77 5.37 0.537 0.537

86.4%R 10 10 14.46 20.19 5.73 0.573

0.570 5.4 0.573100.0 0.570 5.4 2.7

S 10 10 13.36 19.46 6.10 0.610 0.610T 10 10 14.21 19.81 5.60 0.560 0.560

U 10 10 13.58 20.31 6.73 0.673 0.673

100%V 10 10 14.42 21.22 6.80 0.680

0.671 L8 0.680100.0 0.671 1.8 -14.4

W 10 10 14.07 20.83 6.76 0.676 0.676X 10 10 13.12 19.65 6.53 0.653 0.653Y 10 (Q 13.71 19.68 5.97 0.597 0.597

100% IntakeZ (Q • 13.83 19.75 5.92 0.658

0.630 10.10.592

0.614 ".7AA 10 10 15.29 20.90 5.61 0.561 0.56197.5 10.2

BB 10 (Q 13.20 20.24 7.04 0.704 0.704

•••til•o

Q"tfalll0l:Dunnett's MSD valne:PMSD:

0.067011.4

MSD""PMSD""

Mmimum Significant DifferencePercent Minimum Significant DifferencePMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statistically significant in a whole effluent toxicity test.

In_Dunnett's MSD valne:PMSD:

0.082914.1

Lower PMSD bound determined by USEPA (l Oth percentile) "" 12%.Upper PMSD bound detennined by USEPA (90th pefcentile) "" 30%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, ofPMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001h).

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-0l-D04 and EPA-821-B·Ol-005. US Environmental Protection Agency, Cincinnati, OR.

File: sqn101_050812data-uv.x1sxEntered by: J. s~~rRev'lewed by:~

of 110

~ETS~ Environmental TestingSolution., Inc.

TVA I Sequoyah Nuclear Plant, Outfall 101UV-treated

May 08-15, 2012


Larval Fish Growth and Survival Test-7 Day GrowthTest ID: PpFRCR Sample ID: TVA I SON, Outfafl101Lab 10: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring ReportProtocol: FWCHR-EPA-821-R-02-013 Test Species: PP·Pimephales promelas


Conc-%

5/8/20125/15/2012May 2012UV-treated

1 2 3 •D-Control

10.821.643.286.4100

Intake

0.59100.64600.55300.56500.53700.67300.5970

0.64700.62100.62200.60900.57300.68000.5920

0.50700.61600.65900.63400.61000.67600.5610

0.59900.69800.66300.60600.56000.65300.7040

Transform: Untransformed 1-Tailed IsotonicConc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSO Mea. N-Mean

D-Control 0.5860 1.0000 0.5860 0.5070 0.6470 9.929 • 0.6185 1.000010.8 0.6453 1.1011 0.6453 0.6160 0.6980 5.817 • -2.130 2.410 0.0670 0.6185 1.000021.6 0.6243 1.0653 0.6243 0.5530 0.6630 8.163 • -1.375 2.410 0.0670 0.6185 1.000043.2 0.6035 1.0299 0.6035 0.5650 0.6340 4.734 • -0.629 2.410 0.0670 0.6147 0.993886.4 0.5700 0.9727 0.5700 0.5370 0.6100 5.358 • 0.575 2.410 0.0670 0.6147 0.9938100 0.6705 1.1442 0.6705 0.6530 0.6800 1.792 • -3.038 2.410 0.0670 0.6147 0.9938

Intake 0.6135 1.0469 0.6135 0.5610 0.7040 10.171 •Auxiliary Tests Statistic Critical Skew KurtShapiro-Wilk's Test indicates normal distribution (p > 0.01) 0.9571 0.884 -0.4767 0.34009Bartlett's Test indicates equal variances (p = 0.29) 6.13792 15.0863Hypothesis Test (1-tail. 0.05) NOEC LOEC ChY TU MSDu MSDp MSB MSE F-Prob dfDunnett's Test 100 >100 1 0.06704 0.1144 0.00565 0.00155 0.01863 5, 18Treatments vs D-ControJ

Linear Interpolation (200 Resampfes)95% CL(Exp) Skew

1.0

0.90.80.7O.S

~ 0.5c 0.'g0. 0.3

~ 0.20.10.0 ...

-0.1-0.2

-0.30 50 100 150

Dose %

so>100>100>100>100>100>100>100

%PointIC05IC10IC15IC20IC25IC40IC50

Dose-Response Plot

0.8...--------------------,

0.7

0.•

loseC) 0.4

g"'" 0.3

0.2

1-tail,0.05Ievelof significance

0.1

Indopond,olRovl,whV

~II""~.K.,,,."

~'

'"oFile: sqnlOl_050812data-uv.x1sx

Entered by: J. s~erReviewed by: U

of 110

eETS••©,;:; ,,) ,,;o .•.'.....•...,..'; .. '.,,~ EnvIronmental Testing Solutions, Inc.

TVA / Sequoyah Nuclear Plant, IntakeUV-treated

May 08-15, 2012



5/8/20125/15/2012May 2012UV-treated

Larval Fish Growth and Survival Test-7 Day GrowthTest ID: PpFRCR Sample ID: TVA 1SQN, IntakeLab 10: ETS-Envir. Testing Sol. Sample Type: DMR-Discharge Monitoring ReportProtocol: FWCHR-EPA-821-R-02·013 Test Species: PP-Pimephales promelas

Conc-%D-Control

10.821.643.286.4100

Intake

10.59100.64600.55300.56500.53700.67300.5970

20.64700.62100.62200.60900.57300.68000.5920

30.50700.61600,65900.63400.61000.67600.5610

40.59900,69800,66300.60600.56000.65300.7040

Transform: UntransformedConc-% Mean N-Mean Mean Min Max CV"A. N

D-Control 0.5860 1.0000 0.5860 0.5070 0.6470 9.929 410.8 0.6453 1.1011 0.6453 0.6160 0.6980 5.817 421.6 0.6243 1.0653 0.6243 0.5530 0.6630 8.163 443.2 0.6035 1.0299 0.6035 0.5650 0.8340 4.734 486.4 0.5700 0.9727 0.5700 0.5370 0.6100 5.358 4100 0.6705 1.1442 0.6705 0.6530 0.6800 1.792 4

Intake 0.6135 1.0469 0.6135 0.5610 0.7040 10.171 4

i-Tailedt-Stat Critical MSD

-0.645 1.943 0.0829

CriticalAuxiliary TestsShapiro-Wilk's Test indicates normal distribution (p > 0.01)F-Test indicates egual variances (p = 0.91)Hypothesis Test /1·tail, 0.05)Homoscedastic t Test indicates no significant differencesTreatments VB D-Control

Statistic0.97231.15016MSDu MSDp

0.08289 0.14146

0.74947.4683

MSB0.00151

MSE0.00364

Skew Kurt0.34802 -0.3978

F·Prob df0.543 1,6

Dose-Response Plot

0.8,.-------------------,0.7

0.6

lO.6eCl 0.'~C 03...

0.2

0.1

1-tail, 0.05 levelof significance

1"~.F>I'"d,ntR","owtl\'

KoII·I~vc:..n

0 g "" '" '" " 0 .Ii0 N '" "0

c ... '"~

~0()

cI

File: sqnlOl_050812data-uv.xlsxEntered by: J. SumnerReviewed by: -----l:f-

of 110

Page 5 of6


21.6%

43.2%

10.8%

86.4%

100%<

100%Intake

CONTROLUV-treated

Species: Pimephales promelas Date: ()$. .08 •11..-Client: TVA I Seguoyah Nuclear Plant. Out1a1l101. UV-treated --"'=......-'--=--

Dai Chemist .' '

Concentration

Analyst 1-~~~r-::=:---I-:~:r--i"""":~:-""~~-r--:----1

~---~----<fL.,:·

I

I,

IIIf

j),

J

I~

of 110

••e•!D~ E-"-'m-,m-"-"-'T'-'"-',-"-,,,-,,,-,,,-".

Species: Pimeohales oromelasClient: TVA / Se uo ah Nnclear Plant Outfall 101 UV-treated Date: ·01-1"1-

Page 6 of6

Da

SOP AT20 - Exhibit ATZO.3, revision 06-01-11

21.6%

10.8%

43.2%

100%

86.4%


100%Intake

CONTROL I--':'''''''~~__-II-'=:L-_UV-treated

Analyst ....-.,.......;i--.--+,.",.--ii-~--f.....~~-;;..-.----+-~---i;...-._--11---...,..---""";"-

II,I,~

IIIIIIIIII

of 110

1"1!i'~i.!f

TVA / Sequoyah Nuclear Plant, Outfall 101- UV-treatedMay 08-15, 2012

eElS••@I ',',','e"" ',"'; ,', , \,'~,'~o ,> •• ,," " .'

I') Environmental Testing Solutions, Inc.

Pimephales promelas Chronic Whole Efflnent Toxicity Test

EPA-821-R-02-013, Method 1000.0


Project Dumber: 7875

Concentration IParameter Day 0 - I Dav! I Dav2 1- Dav3 1- Dav4 I DavS I Dav6Initial I Final I Initial I Final I Initial I Final I Initial I Final I Initial I Final I Initial I Final I Initial I Final

oR (SUI I 7.881 '-----r.911 7.981 7.811 7.981 7.84 8.071 7.517.8 7.8

""""~'" ~~. .." ,._ ~~, _ •••.""""~;"" ~hh - "'"'='''''''''''' ..."' ..

Control

tnH (SUI I 7.881 7.841 7.981 7.821 7.981 7.851 7.981 7.671 7.941 7.601 7.911 7.631 8.091 7.506.8

10.8%

TemDerature ('C) I 25.01 24.81 25.01 24.91 24.81 24.51 24.81 24.41 25.01 24.51 24.81 24.61 25.01 24.5IDH (SUI - I 7.891 7.901 7.991 7.821 7,981 7.851 7.981 7.681 7.931 7.611 7.891 7.611 8.101 7.50'

21.6%6.8

,Temnerature ("C\ I 25.01 24.81 25.01 24.9\ 24.81 24.51 24.81 24.71 25.01 24.71 24.81 24.61 25.01 24.5oR (SUI I 7.891 7.891 7.991 7.831 7.971 7.841 7.991 7,681 7.881- 7.63T 7.861 1:6018.i1l 7.50

43.2%OOm~ 68Conductivity(~ho~cm)

Temperature("C) I 25.01 24.51 25.11 24.61 24.81 24.61 24.81 24.81 25.01 24.71 24.81 24.61 25.01 24.5oR (SUI I 7.901 7.901 8.001 7.821 7.941 7.881 8.001 7.681 7.811 7.731 7.791 7.651 8.131 7.50

86.4%DO m 6.7Conductivity (umbos/em)

Temperature ('C) I 25.01 24.71 25.11 24.61 24.81 24.61 24.91 24.61 25.01 24.81 24.81 24.91 25.01 24.6oR (SUI I 7.921 7.911 8.021 7.841 7.941 7.891 8.021 7.78 8]51 7.50

7.81 8.1 8.0 7.9~~_J .On' "'~I_ 'An ~:.~.>iiP!l:lt "',,"

DO mConductivi mbos/em 10'::1' ': ~.~

Alkalinity (mglL CaC03)

Hardness (mglL CaCO])Total Residual Chlorine m

Temoerature (0C)

100%

IDH (SUI I 7.941 7.95 '----s.i61 7.527.9

___; .n./ "",,,';;..- k·'.io.E .n.1, "",_"''''~ 1n.. ~.,~. .n.,>

100% Intake

File: sqn101_050812chem-UV.xlsEntered by: C. Jj\onReviewed by:

of 110

IPage \"1.

Page _I_·of_'\.._

Total Residual Chlorine(Orion Electrodl;l MethOd, Orion 97-70)

Matrix: Water, RL = 0.10 mgILMeter: Accumet Model AR25 pH/Ion Meter

IIIII

iET "~ EnvlronmentalTe5ting Solutions-Inc.

Date a~;~:~ I==;~~~:.():a=.:,'l.====Calibration:

1.00 m

Iodide reagent: !.....":LN"""""'<t-9o:.;,,I.,,!QL.- _Acid reagent:. ~~"

Slo e

IL ba oratory contra stan ar :Reference standard Troe value (TV) Measured value (MV) % RS-MV lTV x 100

number (mglL) (mgIL) (acceptable range = 90 to 110%)

~I\l!'~ louL 0.50 0.<2,9- 10"'" To °1.II

LV.OOI

LO.OOI

LO.OO

.t::: D· 00

.cO.oot G

Reviewed by I""'3rl'Date reviewed [$-o-:-;;.t-.,"'''L.:---

(,..1

%RPD = (IS - D) 1[(S+D)/2]} x 100(acceptable range = :1:10%)

Sample characteristics


SampleID

ed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted.

IL ba oratorv contro stan ar, :Reference standard True value (TV) Measured value (MV) % RS- MY ITVx 100

number (mgIL) (mglL) (acceptable range = 90 to 110%)

-:t.N"~ID,A 0.50 O. 'SII, IO'2.,:l.,.-J.

1-"-'~!o!<='=F-"C.ll"",u..':""--::- __ dDuplicateI

I

II

II

I

II

II SOP C8 -Exhibit C8.1, revision 06-01-11

of 110

Page ')..0

Page ----'!:..- of~

Calibration:

~ 0.10 iiigil:: I 1 gg roga I Slope '* '~.\'V

Note: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels of the samples.

Iodide reagent: I-""·a.'k.,. IAcid reagent: ::;;:;

IIIIII

·ETS••@;'j ,"ip;,,'i'\" ~~

-~ EnvironmentalTe5ting Solution5, Inc.

Analyst I_-I!"'~"- _Date analyzed _-'9"':"'.O=I'>"".I:L"'--__

Total Residual Chlorine(Orion Electrodf MethOd, Orion 97-70)

Matrix: Water, RL = 0.10 mgILMeter: Accwnet Model AR25 pH/Ion Meter

z..Lo.o

Co.o

,( 0.001

L.

r

%RPD = (IS - D) 1[(S+D)/2]) x 100(acceptable range = r 10%)

(. t.....



SampleID

L b

Du licate sam Ie reCISIOn:Sample Sample IDnumber

,

a oratorv control standard:Reference True value (TV) Measured value (MV) %RS MV lTV x 100

number ,..,,, (acceptable range 90 to 110%)

0.50 0I!..11b.\l- IllY

I

I

II

I

I

I

I

Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherwise noted.

It dIbLa oratorv contro stan aT :Reference standard True value (TV) Measured value (MV) %RS-MV/TVxlOO

number (mg/L) (mglL) (acceptable range = 90 to 110%)

., .-r • •_"f 0.50 0.#'8 &'1 r. ./•

Reviewed by I--,a(~--::cc-::__Dale reviewed ~--"O",$:...·",a",ll·-,I-,"L=-_

SOP C8 -Exhibit C8.1, revision 06-01-11

of 110

•••l!lJ TS,~ EnvironmentalTestlng SolutiDns, Inc.

Page "Z.S

Page_I_6f~

Iodi~e reagent: I~~",,-,'l:.,,-~-,,,,,-- _

ACId reagent: [£N,._.!!a....:l~""'- _

Total Residual Chlorine(Orion Electrode Method, Orion 97-70)

Matrix: Water, Rl;~ 0.10 mgIL

Date a:;;'~ l-os-=~:O'1"'6'--'\1------11 Meter: Accumet Model AR25 pH/Ion Meter

Calibration:1.00 m IL Sio e


Laboratorv control standard:Reference standard True value (TV) Measured value (MV) % RS- MV lTV x 100

number (mglL) (mglL) (acceptable range = 90 to 110%)

n....S ~ 0.50 O,SIO IQZ.~%

ceUle..Duplicate

Sample characteristics Residual chlorinem IL) :

%RPD = (IS - D) 1[(S+D)/2]) x 100(acceptable ra e =:1: 10%)

<'0.000 rtC7

(0.00$'(7

Sample characteristicsSample Sample illnumber

Sam Ie measurements:


Laboratorvconuolgandard:

numberTrue value (TV) Measured value (MY)

(mglL)%RS MY/TVxlOO

(acceptable range 90 to 110%)

0.50

Reviewed by If,~~,""-,,,---Date reviewed 0510 1'1..

SOP C8 - Exhibit C8.1, revision 06-01-11

of 110

IIII

Page z.J:Page~ of_'&._

iET,~ Env/ronmentalTllstlng Solutions, Inc.


Matrix: Water, RL ~ 0.10 mgILMeter: Accumet Model AR25 pH/Ion Meter

Analyst 1f---''li,,",\1\~ ' Iodi~e reagent: I ----- ]Date analyzed _.!o. rt- . ACId reagent: . """ .r-ao"',<l.hCalibration:

Note: For samples with a residual chlorine of > 1.0 mgIL, the calibration range must be adjusted to bracket the chlorine levels of the samples.I1.00 m IL SI. e

~ OS',"' ....

d dIL ba oratory contro stan ar :Reference standard True value (TV) Measured value (MV) % RS= MV ITVx 100


;Ilo)SS 10'''1 0.50 o. "1co 'i'Z..O,,"II

(0.00'2.1!

c..O.OOIO'2:

(,0. OOC., Z.

%RPD = {(S. D) 1[(S+D)/2]} x 100(acceptable range =:1:- 10%)


1\. '''\.I'j doJ.:I .... , ..~.tcs


Sample ID

Rea ent Blank

DuplicateII

I

IIII


Reviewed by I~Dale reviewed -;M;"'~·I-::O"'.71-z...:------1

d dIL ba orutorv contro stan ar :

Reference standard True value (TV) Measured value (MY) % RS-MV ITVx 100number (mgIL) (mgIL) (acceptable range = 90 to 110%)

-r ..,IC' I"'''' 0.50 o. SIC '03. ''Yo.

IIII SOP C8 - Exhibit C8.1, revision 06-01-11

of 110


Iodide reagent: I'~f-. 6) \0Acid reagent: I fl.6Tl

,

Sio e0.10 m IL

Total Residual Chlorine(Orion Electrode Metholl, Orion 97-70)

Matrix: Water, RL =0.10 mgILMeter: Accumet Model AR25 pH/Ion Meter

It dIL b

Page .30Page~6f~

Analyst 1---=,-;t1t!==:;)AJ~_--1Date analyzed ----'=06~-"·I-=1-_.:....:'"l.::--.-J

Calibration:

eEl••t> ~,d"'''''7':''''' .~ EnvlronmentalTestlng Solutlons,lnc.

a oratorv contro stan aT :Reference standard True value (TV) Measured value (MV) % RS- MY lTV x 100

number (mglL) (mglL) (acceptable range = 90 to 110%)

IJ\15s 10iJ lJ 0.50 n.6z.e IOS.6 7~

IIIIIII

Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) un eSs otherwise noted.

Residual chlorinemIL

.oo~

UJ. OQ:XlO DO

%RPD ={(S - D) 1[(S+D)/2]} x 100(acceptable range =:1: 10%)


Da licate sam Ie recision:

SOP C8 - Exhibit C8.l, revision 06-0l-Il

Reviewed by r--31"...,,--::::-__Date reviewed~ '"1-' '1....

Sample Sample IDnumber

Laboratory conReference standard True value (TV) Measurea va v % RS •.MV ITVx 100

number (mgIL) (mgIL) (accept:Wl't'kange 90 to 110%)

0.50 OSZIt.

IIII

II I

II

of 110

Note: For samples with a residual chlorine of > l.0 mgIL. the calibration range must be adjusted to bracket the chlorine levels of the samples.

Page 0)Page~of~


Matrix: Water, RL = 0.10 mg/LMeter: Accumet Model AR25 pH/Ion Meter

Iodide reagent: E OS I~\t,Acid reagent:. u

SID e1.00 m IL

Analyst C}iiu;-..>Date analyzed D$1".I"'Z.=,yz'----

Calibration:

iETS:~ EnvironmentalTesting Solutions, Inc:.

%RPD = (IS - D) 1[(S+D)/2]} x 100(acceptable range =::!: 10%)


SLO.OOIZZ

Sample characteristics Residual chlorinem IV

101 • iJT

Sample ill

d dI

-rJ It. WBiJ 10)

1\ IIJT

L ba oratoro contro stan aT :Reference standard Troe value (TV) Measured value (MV) %RS-MV/TVxl00

number (mglL) (mgIL) (acceptable range = 90 to 110%)

ll\I"S< IOlo" 0.50 fl.IUL ('\1.'2.7~

II

I

II

IJ: i ,

I

II Note: All samples were analyzed in excess ofEPA recommended holding time (15 minutes) unless otherw-ise noted.

II

Laboratorv control standard:Reference standard True value (TV) Measured value (MV) %RS-MV/TVxl00


h.\~~fOllJ" 0.50 n...fp,q '\1.H.

Reviewed by c:::::M-,-:;---,-=-_-iDate reviewed~. It.- !L

I SOP C8 - Exhibit C8.1, revision 06-01-11

of 110

Page \ of----'-__

Analyst 1-=.:r45=---Date analyzed OS·.os. 12.

Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO,/L

Titrate samples topH= 4.5 S.U.

Time initiated 1_...:1"'°""'''- _Time completed _--',"'''.::''l!..OSL- _

Du licate sam Ie recision:Sample Alkalinity %RPD=

Sample SampleID volume Begin End Total Multiplier (mg CaCO,IL) (IS - D) /[(S+D)/2]) x 100number ml ml ml ml (acceptable range = ±lO%)

06.o~.1l. 5 W'Itc

'I:'l.'l 10.'1-s

3&:1\0<'> 3.3\ Duplicate (B) I D

~·1.1 '1:'.2 3·3 3'i

Titrant normalitv and multiDlier determination:pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier

Deionized Titrant check Begin End Total = (5 ml Na2CO, x 0.05)/E =(N x 50000)/100 ml samplewater reference standard ml ml ml =0.25/E =Nx500

= 4.5 S.U. number number (E) (acceptable ·range = 0.0180 - 0.0220)

'I.' :m" 'it" 'l:WIS 1016 0.0 ~l..o ,'t.o C>.0709 IO.'t

a oratolV contro stan ar :Reference standard True value Sample Alkalinity (MY) %RS-MY/TVxIOO

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) (ml) ml ml ml = 90 to 110%)

:t'l\IU 10.17 100 100 ,~.o ~I., 1.' 10.,+ \00 ,,,o.o~

Ii

Ii

I

I

II

Date reviewed: 100 .0":\42..

31.1

SOP C6 - Exhibit C6.1. revision 06-01-11

RevIewed by:

l

M. 'k

s

afflX SPI e recovery:

Reference standard Spike value Sample Spike alkalinity (A)number (SV) volume Begin End Total Multiplier (mg CaCO,IL)

(rna CaCO,IL) (mil ml ml ~Mml

rllss 1037 So \00 Z'l." ~ -a.a 10.&1 i3

Sample alkaliuity (B) Measured spike value (MV) % R-MV / SVx 100(mg CaCO,IL) MV=A-B (acceptable range

(rna CaCO,IL) = 75 to 125%)

3~ .." 'is.Ot:amme measurements:

Sample volume Begin Eud Total AlkaliuitySamole Dumber SamnleID (ml\ ml ml ml Multiplier (rna CaCO,IL)

0'5-0\.\"2.- tn\o\s/"v 100 3'Z.' 3'.0 '.1 10.'1 '3oS·O'b. ,w, '3....(> ..5.\ '.1 aQ5.0~·1"~ 5·3 \I.Ii '.1 '3O'-\'W.rt- 11.'1 l7.S '-I '3

Q3O"lCi l.k.t.wv.. .sc, 'l.S.Cl ;)0.' S.? ('Z~ I'LO

.

~ "'.(>&.,...-

IJ

II

II

II

Ii

of 110

Page _'--------'of fl

Page _3",-,--'_

Analyst I--~='------lDate analyzed D5'\Z'I~

Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO,1L

Titrate samples topH =4.5 S.U.

Time initiated Ir--=:..""""c.'-----Time completed L_-'-'lt....''-'qL- _

recision:Sample Alkalinity %RPD=

Sample SampleID volume Begin End Total M,ultiplier (mg CaCO,/L) «8 - D) 1[(8+D)I2]J x 100number ml ml ml ml (acceptable range =:1: 10%)

ex::. ,IC.IL 5SvJ 100 U( 25,1 3.3,J; Duplicate (B)

U.l ta.'I 3.'

Titrant normality and multiplier determination:pH of Normality Normality (N) of H,S04 pH Factor or Multiplier

Deionized Titrant check Begin End Total = (5 ml Na,CO, x 0.05)/E = (N x 50000)1 100 ml samplewater reference standard ml ml ml =0.25/E =Nx500

=4.5 S.U. number number (E) (acceptable range = 0.0180 - 0.0220)

S.\ %IolR ,\t" X~S 'ott 0,1 It.Z I'z.1 0.01.07 \O.~

a oratory contro stan af', :Reference standard True value Sample Alkalinity (MV) % RS=MV ITVx 100

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) {acceptable range(mg CaCO,/L) (ml) ml ml ml ~ 90 to 110%)

:l:lIISS 1037 100 100 I'Z:Z lUI 'I.' to.'! \00 100,0%

I

II

I

I

I

Date reViewed: I US·I'·l""

SOP C6 - Exhibit C6_1. revision 06-0 I-II

ReViewed by:

S l

Matrix slJike recoverv:Reference standard Spike value Sample Spike alkalinity (A)

number (SV) volume Begin End Total Multiplier (mg CaCO,/L)(m. CaCO,/L1 (mil ml ml ml

:tMSS 10J? So lot> as. I '31:& t.1 'O.~ 8"1

Sample alkalinity (B) Measured spike value (MV) % R-MV ISVx 100(mg CaCO,/L) MV=A-B (acceptable range

(m. CaCO,/L) =75 to 125%)

3"\ 50 ,0C!.0%

amme measurements:Sample volume Begin Eud Total Alkalinity

Sample uumher SampleID -(mil ml ml ml Multiplier (m. CaCOJ/L)

0'5 .Cf'j, I 'l.1b ImH.stN 100 lOU 1"., '.Z 10." ''i06, 1I,jl-A 3'l.Q 'ls,s C.l '30'6. i).a b 0.0 '.0 t:.Cl 'z1[}';-O'1'12~' ,... \l.' ,., "O'S. otI- t 2ft LJ V Il.' IS.~ '.1 "~01?'?'Z2 ' , 5b ".'1 'ZloS "103 (2.) 6'1..

" '''''7.\.111 Cbln; (L, tOo 'Z'&,.5 U.' '.1 '31 c.\.u Omt..- U., 13".7 '.1 'J...v PPE\c:.o(l)l(.. ,I.- J"\.7 '10" ':1. \ ''''

IJ

I

I

I

I

I

of 110

I

"..Ii>..,®o-',,-'ro-,m-"-"-'T,-stj-,,-S'-'"'-'"-''-'''

Page .It

Page ~ of-",8__

II

Analyst jf-----':NI= _Date analyzed L....:O:>S"-."'I~"'.:.!.I1:==---__

Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaCO,1L

Titrate samples topH=4.5 S.U.

Time initiated I=:-----.. ITime completed ...........;,qs

05011.. /1.

II

Titrant normality and multiplier determination:pH of Normality Normality (N) of H2SO4 pH Factor or Multiplier

d Titrant check Begin End Total (5 ml Na2CO, x 0.05)/E (N x 50000)/ 100 ml samplewater re standard ml ml ml 0.25/E Nx5004.5S.U. number num (E) (acceptable range 0.0180 - 0.0220)

- 0I'!t.,t.

d dI


Sample Sample ID volume Begin End Total Multiplier (mg CaCO,lLl {IS - Dl 1[(S+D)I2]J x 100

number mil ml ml inl (acceptable range = ± 10%)

-OO·\t.A Mil- vV I ,.' IS.' '.0 s,~(I 100

~Duplicate (B) .L 0

''3IS.' ~1.7 '.1

L ba oratory contro stan ar :Reference standard True value Sample Alkalinity (MV) % RS-MV ITVx 100

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) (mil ml ml ml =90 to HO%)

;P\5$ 103'7 100 100 0.0 "I.e 'f., 10.'/ 100 100.0"

II

II

IMatrix slJike recovery:Reference standard Spike value

number (SV)(m. CaCO,tL)

Samplevolume

imllBegin End

ml mlTotal

mlMultiplier

Spike alkalinity (A)(mg CaCO,lLl

Sample alkalinity (8)(mg CaCO,IL)

110

%R-MV/SVxl00(acceptable range

= 75 to 125%)

10."!

Measured spike value (MY)MV=A-B

(mg CaCO,lL1

IOQSoIIIIIII

Sample measurements:Sample volume Begin End Total Alkalinity

Sample number Samplem . (mil ml ml ml Multiplier (m. CaCO,IL)

Os.· <T!:r 1"1.& m~,<, \)\1 2- 100 '2.C."I '2.5 '.1 IO.~ "3o;,,-lr\- 1"1- ~ 31..$ 31.7 '·l -'<+

(6-1:1'\- ILA 4: 31."7 "lit'! '.'t 'If

b~-I\' I""Z.. ~ II 5" 0.0 '.1 '_I C3I "1.0!>'1.1. 0 \ A'6/M """tL- '.1 IU.o 4.11 11:16

\ MU2-01Y\tt. IC.o -zu ''I.' 100

mMc.-omrv U ••. n.1 "1.3 "'t"71/ MfbUl- .J){Y\/V 135.1 "l'U. '1.1 'IS

06-\1.11... SCLJ,:.!-&N !.- C>,() '" f.e II ~.,

ReVIewed by: 6\ Date reviewed: I ClS·IJ.·n....

SOP C6 - Exhibit C6.1. revision 06-0 I-II

of 110

Page .13

Page.s of---'l''''--_

Ana~stl__~~~Q5=- __Date analyzed OS.,,&,"-

Alkalinity (8M 2320 B)Matrix: Water, RL = 1.0 mg CaC03/L


Time initiated I=-:..-......... ITime completed ~

"'·"·rrTitrant normality and multiplier determination:-nU.nf' Normality Normality (N) of H2SO4 pH Factor or Multipliera

Titrant-Deionized .L .1. BeSlin End Total = (5 ml Na2C03 x 0.05)/E = (N x 50000)1 100 ml samplewater reference standard ml ml 1..1 ------.. = 0.25/E ' =,Nx5004.5S.U. number number (E) , (acceptable riiig~ -e.""'''' - RR220)

~OI'R·rt.

d dIL ba oratory contro stan aT :Reference standard True value Sample Alkalinity (MV) % RS= MY lTV x 100

number (TV) volume Begin End Total Multiplier (mg CaC03/L) (acceptable range(mg CaC03/L) (ml) ml ml ml =90 to 110%)

'J:NSS 10'3' 100 100 I,' .,.S ••7 10."1 '01 \Ol.Ot.

%RPD={(S - D) /[(S+D)/2]) x 100

(acceptable range = ± 10%)

Alkalinity(mg CaC03/L)

D

s'II., 16.0\06

Samplevolume Begin End Total Multiplier

ml ml' ml mlSample ill

recision:

Duplicate (B)~..

Samplenumber

Matrix spike recovery:Reference standard Spike value

number (SV)(mg CaC03/L)

Samplevolume Begin End Total

(ml) ml ml mlMultiplier

Spike alkalinity (A)(mg CaCOJIL)

ISo

S l

Sample alkalinity (B) Measured spike value (MV) % R=MV ISVx 100(mg CaC03/L) MV=A-B (acceptable range

(mg CaC03/L) =75 to 125%)

1<>0 'So 'Oo.O~

amp4 e measurements:Sample volume Begin End Total Alkalinity

Sample number Sample ill (ml) ml ml ml Multiplier (mg CaCOJIL)

OS·~·a.6 SGU..:t 6lA.l l4:fO 0&0 CI.' t' Io.~ l04

06dD .t'L l ,." l• .3 tf.... 4fC06..08·1'z, J" \JI '••3 . 11.1 •. f '140

\'"l.l\<"'tC"1 n \ 1VASGi'l lal \ 6b ~1., a.ct 3•• tD "1CI

\1..~.1.S - , z.. 32.9 :SC.I 3.ct ~(

"" ~__ ~~.""·~T~j ,"Z.O~,\.~ 3 3C•• 'iO.-' 'I.! 7.\ 4()~U111 tr1- '(~Asf~ ,~ , . ~o.,· '('1.1 \.1 77\~. ~ot;.1.'- 2- t+'t3 "'t.f 3.' 71\ L..be;,\ •"L.\ ,~ .3 .,~

0.0 1.' 3." " ~v .,.,Reviewed by: Date reviewed: I OS· It I \-z...


of 110

Titrant normalitv and multiplier determination:

-.u.!!. of Normality Normality (N) ofH2SO, pH Factor or MultiplierDeioRlze rant check Begin Eud Total (5 ml Na,CO, x 0,05)/E (N x 50000)/100 ml sample

water reference ..... ml ml ml 0.25/E Nx5004.5S.U. number number lEI (acceptable range 0.0180 - 0.0220)

~ CS1'2..l"l..

Page 3'1

Page f.! of 8'

Time initiated~ ITime completed~~

os· lao.,&.Titrate samples to

pH = 4.5 S.U.

Alkalinity (SM 2320 B)Matrix; Water, RL = LO mg CaCO,1L

Ana~st I~~~CWL __Date analyzed ,---=o..,S,-.t.,:1:,-'..,tL......__

••e®ill,~ =,,,='••='m-..-"-'T.-"'-.,-S.-,,,....:,••-,,,-,,,

Date reviewed: I Cl!)'1'2' rz.... ISOP C6 - Exhibit C6. I. revision 06-0 I- I I

ReViewed by:

d dIb

Du IIcate sam Ie recision:Sample Alkalinity °.loRPD =

Sample SamplelD volume Begin End Total Multiplier (mg CaCO,IL) {(S - D) 1[(S+D)/2]} x 100number ml ml ml ml (acceptable range = ± 10%)

\"2- 50 :J,8 tl)

J, .7.Z ?o." . 3.7 j, 77 '2.~~

M Ix 'k

S l

La oratory contro stan ar :Reference standard True value Sample Alkalinity (MV) % RS=MV ITVx 100

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) Imll ml ml mI = 90 to 110%)

I"NSS' 1~"7 100 100 3.8 11.'1 1.' 10.'" \00 Ic.O.o%

atr SOl e recoverv:Reference standard Spike value Sample Spike alkalinity (A)

number (SV) volume Begin End Total Multiplier (mg CaCO,IL)1m. CaCO,IL) 1m)) ml ml ml 1l61~lt

:PJSS l~'H IDa 8) .,. a. ~& '.of (1) 10.<\ ~leo

Sample alkalinity (B) Measured spike value (MY) % R-MV ISVx 100(mg CaCO,IL) MV=A-B (acceptable range

1m. CaCO,/L) -75 to 125%)

..,1 ",,1.''- --'i3"i' to? ~\l~·r'.o':' 1030 /.amme measurements:

Sample volume Begin End Total AlkalinitySamole number SamnlelD 1m)) ml ml ml Multinlier 1m. CaCO,IL)

\ "'L(f.,(j'\.1.S -rirf\'.::G~ I 0\ 1)\1 2- 50 'ZS-' 7.1.'1 '.S ... 'i\ \, "1.\ 71

\'1..(~1\. "to -It 3 7.1.1.\ n.t. 3011' 7'"n. ao;.cr1. en.. 101

J.r'1'11'A::£:IN I""W \ n,z. '3'.0 7<¥

\ "2- OS,Il'l. '2.1. I'Z J?Co <to.? 3.7 77

1<.~11.'2.\ J, :3"iO.7 Oo/ll.S "3.&' 7q

'"2.Q'i>ln.tA. "iVA wl,t.,} 10\ \ 0.0 '1.\ <.j.t >gs\"2. OSO'\.2.1 I ~ ...\ U 4.2- fl7

\ '2.0<,. \ \. "1.1... ~ 3.

1I.:i 11..&1 '1.\ 85\"'l.. oS (fl.OY '1"JA W~rJ \OII>JT I It. If .,. J ;'l·ot V 111

I,

I,

I

11

I'"II

I,IIiI

'iiIrI

I

I,I

II,

of 110

II

••~

@

10,~ E-"-iro-,m-"-..-'T.-"-,.-,,-,,,-,,,-,,-",

Page J$

Page'S 0[----<6'--_

II

Analyst I ~Date analyzed o.s.I'Z"I~

Alkalinity (8M 2320 B)Matrix: Water, RL ~ 1,0 mg CaCO,/L


Time initiated I:::=---.... ITime completed ~

o'·IZ,·a.

III


D~' , dNormality Normality (N) of H,S04 pH Factor or Multiplier

elODize Titran.. ~" ck Begin End Total (5 ml Na,CO, x 0.05)!E (N x 50000)/100 ml samplewater reference standara ml ml 0.25/E Nx5004.5S.U. number number facceotable range 0.Ot80 ~ 0.0220)

.:ItS <IS·/Z·tto

d dIL ba oratory contra stan ar :Reference standard True value Sample Alkalinity (MV) %RS=MV/TVxIOO

number (TV) volume Begin End Total Multiplier (mg CaCO,/L) (acceptable range(mg CaCO,/L) (mil ml ml ml = 90 to 110%)

~s~, 100 100 ".3 1:'.0 '1.7 10.'/ 101 10/.oK

IJ

IIwJ


Sample ID volume Begin End Total Multiplier (mg CaCO,/L) {IS ~ Dl /[(S+D)I211 x 100mil ml ml ml (acceptable range =:l: 10%)

2- -Z':.O 'Z..··Of 11)S

81\ 3.".l.. .It D

1. 'I. "I '33. 'I 'I.e 13 'Vi~

Ii~I

I

Matrix sDike recovery:Reference standard Spike value

number (SV)1m. CaCO,tL)

100

Samplevolume

ImllBegin

mIEndml

Totalml

MultiplierSpike alkalinity (A)

(mg CaCO,/Ll

1'0

Sample volume Begin End Total AlkaliuitySample number , Sample ID ' Imll ml ml ml Multinlier 1m. CaCO,/L1

\ 'l. os.1\.~ 1\1Al,\J lW 1\)1 INn, 50 38.' 11.1'2.' ~.o l"» 16."1 ,«3

1"l.D'i>tn OS'· ';-v'AWIQ,v II'?> , "Zo. , I c.".Q cu. '47

\ 'l. o!.O"\ ."Z.C\ I Z- 0.0 '1.3 't.3 f.Cr

I "Z.OiOl \. '2.i.I .v 3 1.f.'3 ~... ct.1 is\ "l.0<;, l!'"\. til::. -rV'A- W~<U II~I~ I 1I.~ I"Z.. , 7.'" 77n (j';,O"\.~ I ;i!- \'2..1 15.'1 3.9 "7'1

\"l.O~\'.1.' V 3 ,I; 1$, " 1 ...1 '1.0 n\., ''''''''''".m r:Art.ITl1,llt I 100 ~1.S -a1.l{ 0." 'f.t1

n.(lS. 10. a1 ~ z. 1,,- 1.'t." c:u. -z,9 .. oz."

SOP C6 - Exhibit C6, I. revision 06-01-1 I

IIIi

i

IIII\

Sample alkalinity (B)(mg CaCO,/L)

Sample measurements:

Measured spike value (MY)MV=A~B

1m. CaCO,/L)

17

ReVIewed by:

% R-MY /SVx 100(acceptable range

= 75 to 125%)

'17.0%

Date revIewed: I ll&·,Z·!"L.

of 110

Titrant normalitv and multiolier determination:

Il1l.2!. Normality Normality (N) of H,S04 pH Factor or MultiplierDeionized check Begin End Total (5 ml Na,CO, x 0.05)/E (N x 50000)/100 ml sample

water reference sian ml mi ml 0.25/E Nx5004.5S.U. number number E) (acceptable range 0.0180·0.0220)

-C.f.ll.·~

Page 3C

Page , 0[---"'---_

Time initiated I............... ITime completed . ~

...·'1·.\Titrate samples to

pH =4.5 S.U.


Analyst I-......:n.s"""--Date analyzed OS.'Z.,t

....@

'!'I$

~ E="='W='W=-"-""',T'="''--''C:-SO,c-'uti=''=-',,-".IIIIIiIiIIiIi

Laboratorv control standard:Reference standard True value Sample Alkalinity (MY) %RS-MV/TVxIOO

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) (ml) ml ml ml ;:: 90 to 110%)

;r1'lo6 16.,,-7 100 100 35.'t '1'1. '1 '1.5 ,e. "f "1"1 '1'.0%

Sample Alkalinity %RPD=Sample volume Begin End Total Multiplier (mg CaCO,IL) (S - D) ![(S+D)i2]} x 100number ml) ml ml ml (acceptable range =::1: 10%)

Z.$' z.i 10."

si.'10.<0

~D

~.9 5.' Z.~ ~"I


Matrix soike recoverv:Reference standard Spike value Sample Spike alkalinity (A)

number (SV) volume Begin End Total Multiplier (mg CaCO,iL)(m. CaCO,IL) (mil ml ml ml

:l'Mli,S 10"57 Sr; 160 'Z.' 10.3 7.5 10.,,/ 7'Sample alkalinity (B) Measured spike value (MV) % R-MV 1SVx 100

(mg CaCO,IL) MV=A-B (acceptable range(mg CaCO,IL) = 75 to 125%)

oz." "" "i.l>,",Samole measurements:

Sample volume Begin End Total AlkalinitySamnle number Samole ID . (mil ml ml ml Multiplier (m. CaCO,IL)

I ~ ~.. "-". I f"'\ :5 .L ,('1\".. I lOa 10.3 ISot ..., 10.1./ Sfn.~ICl.O'\ I z.. LS.l. ~O.() 'i,t So

1'2.~\" .08- .t, .3 ,I- z...o lu./ S.l 53\ 7 h"-" II. l\"" C;:lU(.l.l. \ )l,<; t,s: , Z'f.:t ~., ~) 170

\ '2,1"'-' rl. It%.. z.. l.1.l: 3'1.$ 5:3 -z.z.o,") ~\'1 .en. 3 I.- l ... S 31.0 Cf.S "0'1.cSoa.o \ ~o~ I 100 '3'1.0 "\"'., 3.( 37

1<'OS'.IO .0 I \ z. n.'· lK1 to .. CiS\7..~ 12,. CJ \ .J/ ..3 ,/ c ... ,... '.4 V chr..~ (pi-

Date reviewed: I Cl S • ,1. -I"LReVlCwed by:

II

of 110

Page .. ,irN""I11

•..l'l<i1IJfJ~ ="=Vlro=,m=-'-:-'''-='T'-''''--"-:C"-:-,,,=,,,=-,,:--",

Analyst It--....,;r;"'~"---Date analyzed L_o=~""-,Ic:::Z....:'I-,"t,--__

Alkalinity (SM 2320 B)Matrix: Water, RL = 1.0 mg CaC031L


Page --,?~_.of 'i/

Time initiated E ITime completed ::::::;;

~·I'7·'l:

ITitrant normalitv and multiolier determination:

..f..H of~,

Normality Normality (N) of H,S04 pH Factor or MultiplierDelO t check Begin End Total (5 ml Na,C03 x 0.05)/E (N x 50000)/100 ml .ample

water reference • ml ml ml 0.25/E Nx5004.5S.U. number number iEl (acceptable range 0.0180 - 0.0220)

, : 1"7-s.:a oS·I'l..

11

I,

IiIJ

Laboratorv control standard:Reference standard True value Sample Alkalinity (MY) % RS-MV /TVx 100

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) (ml) ml ml ml =90tollO%)

~S 103, 100 100 '.'i 'C.O ,., 10.1.1 10C> 10O.O'i'd

Sample Alkalinity %RPD=volume Begin End Tolal Multiplier (mg CaCO,IL) {(S - D) /[(S+D)I2]} x 100

ml) ml ml ml (acceptable range =:1: 10%)

.:\"5 1(.0 18.9 'Z.1/ I~)S

I'Z.O

~ ,it t.' J D.,.. "lol." 110 ~.m

t

k

So l

Matrix sol e recoverv:Reference standard Spike value Sample Spike alkalinity (A)

number (SV) volume Begin End Total Multiplier (mg CaCO,IL)(mg CaCO,lL1 (mil ml ml ... ,.....ml

'J:NSS 103'7 OIbO ~S '-If.l ~ 7."1 Ii) 10.~ 310;",5

Sample alkalinity (B) Measured spike value (MY) % R=MV /SVx 100(mg CaCO,/L) MV=A-B (acceptable range

(mg CaCO,IL) "" 75 to 125%)

lit> ~Cli:I IC6,OYo

amme measuremen 'S:

Sample volume Begin End Total AlkalinitySample uumber SamnlelD . (mil ml ml ml Multinlier tm. CaCO,IL)

11. t,<:.. II' .0 1ioIc.. r() Z dI'S -.:1." ~... .l '2.'7 ('I) \0•., 110

I 1. Cl~I1..,01. J., 3 J., 11.5 3'f."/ '2." I I'l.o

I 'l.oc:. ", oS ~rcPl nt""'& I Z JIo/.1J '10.3 1:.'1 ISO) '3IDO

\1. UbIO.OSv

I '2. "1o. :s Cle;. " c; , lZ.oo• ?Cl<"I'l.. 6S, l 3 0.0 ,.\ '.\ '3t.oC)

I" O<''''./ll:, 'Po ~cJo,billt. 1 '.1 10.1 '1.0 ~IClO

I'J "(./h./l(,, z.. 16.1 1"1./ 't.O l:1~~

1'7/l<' " • 0(",,. 3 -' 11.\.1 I' q 3.'5 I' ~OOCl

126<;'Ol.0'-l AACQ.1w ( 50 17.'1' 1.1.S 3., ('1) >/I 7S

II

Reviewed by: Date revIewed: IDS' 12.·11.

SOP C6 - Exhibit C6.1. revision 06-0 I-II

of 110


_.e!I ofTitrant

Normality Normality (N) ofH,S04 pH Factor or MultiplierDelOD check Begin End Total = (5 ml Na,CO, x 0.05)/E = (N x 50000)/100 ml sample

water rei'ere>rr- standard ml ml ml -0.25/E - Nx5004.5S.U. number nnm IE) (acceptable range 0.0180 - 0.0220)

~ OS·I'Z·I'Z

Page 3'5

Page 11 of--,,~,--_

Time initiated 1-...............----==--=:,...---Time completed ~

C\lrI.4t.Titrate samples to

pH =4.5 S.U.


Analyst 1--".:s;...,;SS:::..---Date analyzed ...;o",s,",.:.>.n~'I",t.,---__

•••~

@> ==-~=-::-:-=-o£nvlronmentalTestlng Solutions, Inc.

I

Laboratorv control standard:Reference standard True value Sample Alkalinity (MV) % RS-MV ITVx 100

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) (ml) ml ml ml = 90 to 110%)

;rN$S 1037 100 100 Z,I.S 3\.'l 't.? \0,4 101 101.0%

Duplicate samPle precISIon:

.,. Sample Alkalinity %RPD=Sample ID volume Begin End Total Mnltiplier (mg CaCO,IL) «S - D) 1[(S+D)/21l x 100

number ........ ........ (mil ml ml ml

~"'-- S

Dnplicate (B) ............... D..........

Matrix spike recoverv: -..............Reference standard Spike value Sample ..........

~Spike alkalinity (A)

number (SV) volume Begin End Mnltiplier (mg CaCO,IL)1m. CaCO,IL) (mil ml ml ......

~"'-

Sample ,dkalinity (B) Measnred spike valne (MV) ~XI00(mg CaCO,IL) MV=A-B (ace ble range

(m. CaCO,IL) =75to %)

~ ''It·R.Sample measurements:

Sample volume Begin End Total AlkalinitySamnle number Sample ID - (mil ml ml ml Multinlier (mg CaCO,IL)

I" n<:. I n.N Draro Ivr ~ 6D 31.t. 'J'l. , Jot! '1.'1 10 If "71

I" l'I<:.".O" L 3 -\; 3'1.' 3t.~ '1.' .L 75

n<'.I\.\'~ InHC" LllP ICO 38.2 '1'1. ~ '.0 ,,/ CZ~

--- ,_ 0$.12.. 12.

ReViewed by: Date reviewed: I OS • IZ •11..


of 110

Analyst 1-...::sG:..=--Date analyzed .S,O$· I'Z

Total Hardness (8M 2340 C)RL = 1.0 mg CaCO,/L

Page' --"'~'----_

Page of--'--__

Time initiated I 01J~ _

Time completed __0;;..'1.;;5",',,- _

Titrant normalitv and multiplier determination:Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier

reference standard ml ml ml =O.Z/E =(N x 50000)/50 ml samplenumber number (E) (acceptable range = 0.0180 - 0..0220) =Nx 1000

S\ll "'ft ~NC'\\~ 0.0 lO.O '0.0 0.0,2000 ZO.O

It dILba oratory contro stan ar :Reference standard True value Sample Hardness (MV) %RS-MV/TVxIOO

number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range(mg CaCOJIL) (mil ml ml ml = 90 to 110%)

XN,ss \033 40 50 \0.0 IZ.O Z.O ~o.o 4(0 100.°%recision:

Sample Hardness %RPD=Sample Sample ID volume Begin End Total Multiplier (mg CaCOJIL) {IS - D) 1[(S+D)/2]} x 100

number ml) ml ml ml

050 12- SSW SO It.o 1'1.0 a.o ~.()S

t.IO

,I..Duplicate (B) D

40l'to IC.o ~o

M.tix 'k

S l

ar SOl e recoverv:Reference standard Spike value Sample Spike hardness (A)

number (SV) volume Begin End Total Multiplier (mg CaCOJIL)(m. CaCOJlL1 (mil ml ml ml

;II.ISS 1033 ~O SO 1<./.0 '4.0 .... tl 2:0.0 'Sf>

Sample hardness (B) Measured spike value (MV) % R-MV ISVx 100(mg CaCOJIL) MV=A-B (acceptable range

(mg CaCOJIL) = 75 to 125%)

"to '{O 100'09'.amme measurements:

Sampie volume Begin End Total HardnessSamole number Sample ID - (mil ml ml ml Multiplier (m. CaCOJIL)

TV=ND Blank50 0.0 WI)(should be - 0 m. CaC031L) O.Q ".0 'ZOo ()

O'\S-DJ .12. l'h I5.IAJ 1$,0 n ... 't.ll 1119'0'0- o'6·,zJI 1.'2.1\ ·U.f 'f.ot ~'lt

05·()3-IM 'Z'.S' 31. 'Z. If.<l it(}\·OO -IL. .11 , '31.1- 'IS.S 't.' • "11.

-------- J\SS Q5.01S·l2-

Note: If >15m! of titrant is used. sample must be diluted. Revtewed by: [!.-I/}...(;"';\.....='-___ Date revIewed IOS' Of·, L


of 110

%RPD={(S - D) l[(S+D)l211 x 100

1'1

Spike hardness (A)(mg CaCO,IL)

Page _-'---_,of &

Time initiated If-.!o01aJ'c::li/l----'-~.I.Time completed __1-=!"'2"'3=- .

s

DI'" .12..0

".1 'Z.o

Page --,,7__

Total Hardness (8M 2340 C)RL ~ 1.0 mg CaCO,/L

Begin End Total Multiplierml ml ml

1'1.1 It.\ 1.1.0 l'l.~

HardnessBegin End Total Multiplier (mg CaCO,IL)

ml ml ml

50

Samplevolume

ml

Samplevolume

(mil

a d

SamplelD

recision:

I

Duplicate (B)

Samplenumber

••iii$~

~,=,'-"m-,m-,-,,,-"-,,"-,,-S-'''-'''-''-''-'.

Analyst 1_....:)$= _

Date analyzed _CS.:.=:...._I'3.=....;.I:..:'2.=-__

L h

Reference standard Spike valuenumher (SV)

(m. CaCOJIL)

Matrix spike recovery:

Titrant normali(y and multiplier determination:Titrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier

reference .standard ml ml ml =O.2IE = (N x 50000)l50 ml samplenumber number iEl (acceptable range = 0.0180 - 0.0220) =Nx 1000

;I:... /c' '"'is :2:1'1.tS "111 0." 10. \ 10·1 0.0'''8 . I"I.~

a oratorv contro stan ar :Reference standard True value Sample Hardness (MV) % RS = MV lTVx 100

number (TV) volume Begin End Total Multiplier (mg CaCOJIL) (acceptable range(mg CaCOJIL) (mil ml ml ml = 90 to 110%)

;J:lIIS5 1033 40 50 10.) It.l t. c> \'1.' ,",0 100.0%

Sample harduess (B) Measured spike value (MY) %R-MVlSVxl00(mg CaCOJIL) MV=A-B (acceptable range

(mg CaCo,IL) = 75 to 125%)

/.fa 31 17.S%Samole measurements:

Sampie volume Begin End Total HardnessSamole number SamolelD - (mil ml ml ml Multinlier (me CaCO,lLl

TV~NDBlank

So Nl)(should be~ 0 me CaCO,IL) bou 0.0 0.0 flU

feHI.I2. A .11 H-S vJ If.\ 'l.'I.., ~.S' if05 ·11.n6 z:z.c 'Z7.1 &l.S 6'05.l>':)·11- l7.1 31.' .... 5 rtfos·i)'1·a..,q . :i\. , ~,.o 'i.'I S7OS· OClJ·/l. p.,. II 3'.6 "Io.S ...5 S1"1

Q'b3<l1 {I In.••",. ....Il1. 40.S '17." "., 1"'0

\ '2.0~2.I_O l C!Ac-bma- 0." 't.5 ....$ 31eJ. ql. DmtV '1.5 '1.0 '1.5 .,

I.- ~AL Dm/t... '1.0 11.5 "'.$ 1I 8'''

IIIIIIIIIII

Note: If >15ml ofUtrant IS used, sample must be diluted. Revtewed by: 1----.&",\\-.-- Date reViewed I Os.-I'.!>-n....


of 110

Titrant normality and multiplier determination:~_. Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier.....

reference stanoalU_.

ml ml = 0~2/E =(N x 50000)/ 50 ml samplenumber number ~ (acceptable range = 0.0180 - 0.0220) =Nx 1000

~_'3·'t

Analyst I_~~ _Date analyzed oS· '3- t~

Total Hardness (8M 2340 C)RL = 1.0 mg CaC03/L

Page _'=-_Page ----""Z"'--_of ,

Time initiatedE ITime completed~~:

oS'I'3'l't

a dILba oratory contro stan ar, :Reference sta~dard True value I Sample Hardness (MV) % RS = MV / TV x 100

number (TV) volume Begin End Tot~1 Multiplier (mgCaCOJ!L) (acceptable range(mg CaCOJ/L) (mO ml ml ml = 90 to 110%)

:S:~ 1033 40 50 ".S. '-S.S ~.c> '1,t Coto 100.°"recision:

Sample Hardness %RPD=..Sample ID volume Begin End Total Multiplier (mg CaCOJ/L) {(S:. D) /[(S+D)/2]) x 100

ml ml ml ml

2.. &0 '1$.5 'lct.4 ....&1s ·87D

'97l'.1 t't.3 ....44

Ml . OkatrIX SPI e recovery:Reference standard Spike value Sample Spike hardness (A)

number (SV) volume Begin End Total Multiplier (mg CaCOJIL)(mgCaCOJ!L) (ml) ml ml ml

'XNS.s .'0;,3 tto So '1..1 1.'.1 ' ( .. 't'. "." \10

Sample hardness (B) Measured spike value (MV) % R = MV / SV x 100(mg CaCOJIL) MV=A-B (acceptable range

(m2CaCOJ!L) =75 to 125%)

,t{1 &(3 . 'o7.S~otS lamp. e measuremen 'S:

Sample volume Begin End Total. HardnessSamp·le number Sample ID (mi) ml ml ml Multiplier (mt! CaCOJIL)

TV-?~Rhmk

~OItI3""t(should be omil; CaC03/L) -I .... f1

O~ .. tn.r, 'Z. A m"~lhJ l So 1.'.3 30..7. r.tt'" .,. ". C?6t;...(M-,'1- ~ 30.7 3S.t tof.S .ttt)S·b4\"I1..A 4 15.l. 11.7 .....5 I'os-n.. tLA 6 31.."7 "".1 ·....5 SCI

~·U"'1.& ... t ~ e.te.t. 'Z ",.7 ...·.5 aCl''~6__ cn.. a' (IA ~~tJ'O\ \ 0.0 '1'.1 ct.\ ·e,\'Z.~~64a -."LS \ '2. 'I.' ,;r. .....1 II.l~ .J1-~~\J ."~ ~~ jI .3 .•'2. tZ.3 'I.e 'S"....., 1.~a1.G1,.... -r,"A~ ,Jtn- , .~~ \'1.' le.3 "f.o ,I '71

Note: If >15ml oftitrant is used, sample must be diluted. Reviewed by: 1--...,(31bHr---- Date reviewed I .0 \-" •\1.....

SOP C7 - Exhibit C7.1. revision 06-01-11 '

of 110

Time initiated I............. ITime completed ~

oS"J.ll

eEl•l!I@@ ~_:o, ~.~ Envlronment~rTestJng SolutIons, Inc.

Analyst 1_-';:)1$=__---1Date analyzed _~-=-.l",3'-'l:.."t.=--_--,

Total Hardness (8M 2340 C)RL = 1.0 mg CaC031L

Page

Page 1

:1 of----"&=--_

Note: If >15ml of titrant "used. sample must be dduted. ReVIewed by: I ~(<J;)Q Date reViewed I l)!>.I'~'l"Z_


d dI

'k

L h

Du Iicate sam Ie recision:Sample Hardness %RPD=

Sample Sample m volume Begin End Total Multiplier (mg CaCO,IL) {(S· D) 1[(S+D)I2]J x tOOnumber A ml ml ml ml

$\J Iwr 1- 1t.'J nit ·u ~'·fs il1"1.0S 0

~Duplicate (B) .y D

1$12~.q 't,.s 'tol 'V

Titrant normalitv and multiolier determination:rmaIitv check Begin End Total Normality (N) of EDTA pH Factor or Multiplier

reference standard m. m. m. 0.2/E (N x 50000)/50 ml samplenumber number IE) (acceptable ran -0.0220) Nx 1000

~...CI$·I'·I'Z.

S t

a oratory contro stan ar :Reference standard True value Sample Hardness (MY) %RS=MY/TVxlOO

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) Iml) ml ml ml = 90 to 110%)

:rNSS tc>:J3 40 50 ".3 It. '3 't.o lif•• '10 '0(>.0%

air/X SPI e recovery:

Reference standard Spike value Sample Spike harduess (A)number (SV) volume Begin End Total Multiplier (mg CaCO,IL)

1m. CaCO,IL) Imll ml ml ml

J:NSS 1633 '10 5"0 ~'Z.'t foe." 'Cl let. S l'ZoO

Sample hardness (B) Measured spike value (MV) %R-MV/SVxlOO(mg CaCO,IL) MV=A·B (acceptable range

1m. CaCO,IL) ~75 to 125%)

il 3" Q7.S%amPle measurements:

Sample volume Begin End Total HardnessSamole number Samolem Imll ml ml ml Multiolier 1m. CaCO,ILl

TRlonl<

~OS'IHZ(should be . 0 m a ,

\.,ne. I'. '2. \ -r'A-SQ"':~ 3 50 ?8.~ 137.... '1.0 l't.t' ?r

'7 ~,,"LII I ,vA $ ......0. Uli I 32.1{ 3',5 't,I fl

110'SO<;.1.~ I Z. 1,.5 LlO. , 4.1 81

\7.1Ic.. n.?f'I ..... 3~O.' 1ol'4•.., ",. I III

,., ...e:-,..,.n ii/A ~tJlOlmu\l1 4 ....7 'It.t If. I 81

I-z.OSO"'.'1.'- I 2. c..o ....0 10(.0 7"l'1.tI"'II.? I .l- 3 . "1.0 ~;I ..... 'I

.7""' ...... "'" "filA wB/\) 10 I I ~.l It.S '1.1{ (/7

1'1. tl c;. 11'\ .1.1 J z. ~I,1'Zo.S te. 'I lj .1.{ V ~?

IIIII

II

IIIII

I

II

of 110

Time initiated ,............... ITime completed "S\.

O.$'."'.R.

Total Hardness (8M 2340 C)RL ~ 1.0 mg CaCO,lL

d d

d ['r

1

Analyst 1---'--:r0---Date analyzed ---=c>::;S=.'..:c"=.'..,Ic::t.,--_

recision:Sample Hardness %RPD=

Sample SampleID volume Begin End Total Multiplier (mg CaCOJiL) {IS - D) 1[(S+D)i2]} x 100

number ml) ml ml ml

.1;1- "lilA weN 101 ~ So u.'1 '1..5 \U s fer11.'1

l- Duplicate (B) .L ....$ ~0

'2.1.'1 1.1. 'l ll'l

• Page 10..'iil,"@ Page e.{: of ,'B

L b t

s [

Titrant normality an mu ttouer determination:.I:ttrant Normality check Begio End Total Normality (N) of EDTA pH Factor or Multiplierreference ml ml ml 0.2/E (N x 50000)1 50 ml samplenumber number (acceptable range 0.0180 -.0.0220) Nx 1000

~ON1'1~

Note: If >15ml oftitron! is used, sample must be diluted., ReVIewed by: I__--j·~~--- Date reviewed I Oi.·I~. n_SOP C7 - Exhibit C7.1. revision 06-01-11

a ora orv contro stan ar :Reference standard True value Sample Hardness (MV) % RS - MV lTV x 100

number (TV) volume Begin End Total Multiplier (mg CaCOJiL) (acceptable range(mg CaCO,IL) (mil ml ml ml - 90 to 110%)

.~s.s 1013 40 50 IC."I I$. 'I 2.0 1"I. t. . 40 100.0%

Matrix soike recoverv:Reference standard Spike value Sample Spike hardness (A)

number (SV) volume Begin Eud Total Multiplier {mg CaCO,IL)(me CaCOJiLl (ml) ml ml ml

. .t'~a.s \03:3 4e:. I So 13.'1. Z'l·' '.I.f 1'I.f 130

Sample hardness (B) Measured spike value (MV) %R-MV/SVxIOO(mg CaCOJiL) MV=A-B (acceptable range

(me CaCO,IL) = 75 to 125%)

8'1 '41 10'2:.5%Qmme measurements:

Sample volume Begin End Total HardnessSample number SampleID ' (mil ml ml ml Multiplier (m. CaCOJiLl

Blank 5U!> OS 1~·I"C..(should be 0 mg CaCOJiL)

\'1.()\tn,o'i 'hi 1\ WB.-) IUII\lT" , 50 'l.'I.8' 'S't.1 4.3 ''1.i' 35, .. ~"".1.2' 2. 3";/ .l8.s &1.'/ "\7

\ Z.OS\\. 'I.~ ,., :2> 3'.5 '\"z.- 'I.J 8S1"Z.tt;, lI"'\ • 0'5 IiiA IA1QN II". I I '-I Zo.t '17:Z. ....~ . tn

1"1...(1;. 0"\ .-z.4 2- 0.0 '1.5 '1.5 8'1

\ "2.O~l\. 2.t.l '" 3 4.S '.'\ 'Vf ..,n.~IM.lI(. .,..~Aw8...,)M.I.".· I fl.'! 11•• 1,' 17

., .. ,,"' ...'" .M '2. It.'ll ~.'1 ..., ~l

,7,rJC.1 \.~ 3 .. I'.' ~o.S 3.'1. , II' 17

of 110

Titrant normalitv and multiplier determination:...J:.i!rant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplierreference d ml ml ml 0..2/E (N. 50000)/50 ml samplenumber number lEI (acceptable range 0.0180 -.0.0220) NxlOOO

~()S·U·\~

~ET"@@. ,:@ k,.;":I'ili'Jl f,~

~ EnvlronmentaITestlngSo[utlons, Inc.

Analyst 1-'-':l1"-'\.B""----Date analyzed ---=0",5_",-,1,-'.:..''t.=.....__

Total Hardness (8M 2340 C)RL ~ 1.0 mg CaC031L

Page --'.jll<-_

Page S of---"''---_

Time initiated I"==- ITime completed. . ...........:!I'\R

qs. '''I"'£.

Note: If >15ml oftttrant is used, ~ample must be diluted. RevIeWed by: I__~d-\<;.I-,I--_- Date revIewed I 0 ~. \ .. ' \ "'L..

SOP C7 - Exhibit C7.1. revision 06-01·11

Laboratorv control standard:Reference standard True value Sample Hardness (MV) % RS - MV lTV. 100

number (TV) volume Begin End Total Multiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) (mn ml mI ml - 90 to 110%)

%/olas 103'5 40 50 110.\' ll.7 I. 'I 14.9 31 'i$.0%

'k

Sample Hardness %RPD=volume Begin End Total Mnltiplier (mg CaCO,IL) (S - D) 1[(S+D)121J x 100

mI ml ml ml

n .• 0.1S ".01 '1:1..1

'"'Z., D'i.On., 0.1

MatrIX sp, e recovery:Reference standard Spike value Sample Spike hardness (A)

number (SV) volume Begin End Total Mnltiplier (mg CaCO,/L)(m. CaCO,IL) (mI) ml ml ml

r~s 10'33 lib loS l.l..i' t'l.'I 1:.1 11>l'Mt '3

Sample hardness (B) Measured spike value (MV) % R=MV ISV. 100(mg CaCO,IL) MV=A-B (acceptable range

(mo CaCO,IL) = 75 to 125%)

~.O "7"1 '1'.~Samole measurements:

Sample volume Begin End Total HardnessSample number Samnleill - (mn ml ml ml Multinlier 1m. CaCO,/L)

,~" -~~ Blank- ....05·'3·'2,(should be 0 mo CaCO,IL)

\"1. O~ln. tn iOflUYlIi .llI ~ So t'l.'t ~S.1' _ 0.'1 I,.i' It\ '2.flc;.,") • In .J, -3 l-$., U.'7· 11.'1 18

\"1. Q$.OI. '0 ~t"\CA-~ \ "l.'-' ,"1.' . %.'1 57

\1..lF-. II... 0'\ I 2- ~1.' ')l.3 ·Z."7 ~"v~6'-\

1'1.11<;"1'1.. 0& .It .3 ,It n.1 l'4. 'I -Z;' ffJo'VSr' Ii~

'''2.Cl" II , •Cf'. ~rUL IlL I 10 3'4." 3'·7 \.8 6) Ifo\1.OSl0.C& { ~ 3'.7 'SS•• OZ. , I 't.lo

l"tfl$.\~ .~ JI -3 J1 3fl.'i . '10.7 1.'1 ~ 1'0

,., 11'\ M.O ( ~d I 50 '10. 7 "IU (U ,II S"l

I

Ij

iVI~1,i~,I,I,III

of 110

Page 1"1-

, of--,',,-_Page

Time initiated E:;;:: ITime completed c= ---:r~

0$0 "·I~

Total Hardness (8M 2340 C)RL ~ 1.0 mg CaCO,1L

recision:Sample Hardness °.loRPD =

Samplem volume Begin End Total Multiplier (mg CaCO,IL) (IS • D) /(S+D)/21l x 100

ml ml ml . ml

$'6 1.,\ '.S 4.' s "II...., D

"I,.$ 11.1 ~ QS·I3-rl.

t I / It d

l

L b /

s

Titrant normalitv and multiplier determination:TUrant Normality check Begin End Total Normality (N) of EDTA pH Factor or Multiplier

reference s :. ml ml 0.2/E (N x 50000)/50 ml samplenumber number {" ccentable range 0.01S0.0.0220) Nx 1000

QS·I!·I"Z

Note: If >lSml oflitrant is used. sample must be diluted. Reviewed by: I-----,J...C--- Date reviewed I 0$.\"\-\1-

SOP C7 - Exhibit C7.1. revision 06·0 I-II

a ora orv con ro s an ar :Reference standard True value Sample Hardness (MV) % RS - MV ITVx 100

number (TV) volume Begin End Total Mnltiplier (mg CaCO,IL) (acceptable range(mg CaCO,IL) (mil ml ml ml = 90 to 110%) .

"%NSS 101";$ 40 50 6.0 1.'1 1·1 \'1.'6 lS 'is.O%

Matrix spike recovery:Reference standard Spike value Sample Spike hardness (A)

number (SV) volume Begin End Total Mnltiplier (mg CaCO,/L)(mo CaCO,/L) (mil ml ml ml

":l:'NoSS 1033 ,",0 Sc> '.5 13.1 ,., l'l.t I~O

Sample hardness (B) Measured spike value (MV) % R=MV ISV x 100(mg CaCO,IL) MV=A·B (acceptable range

(mo CaCO,JL) =75 to 125%)

'II 3' "17.5%amme measuremen 'S:

Sample volume Begin End Total HardnessSample number Sample ID - (mil ml ml ml Multiplier 1m. CaCO,IL\

T" .,~ Blank at8 OS·'3·1"t.(should be omg CaCO,IL)

11.0C,\'2,. n \ FiN.uwd -;J So '"3.1 \1.3 &.l.z. ,,,.~ f3':.J

'OO ,,' ".o~ fhlrllY1 I 1"7.3 '8'.'1 1.1 Z~

\ L 0(, Itl • /t1. I l- lSi." ~I.s 3.\ C/

"_l'ftl1..O'L J, 3 , ·u.s ZS.t 1.'7 ~ 73---- .

---- <J~ 0$. \1"2.

IIII


Appendix D

Reference Toxicant Test and Control Chart

Page 85 of 110

of 110

•••tiPo~

Pimephales promelasChronic Reference Toxicant Control Chart

Organism Source: Aquatox, Inc.Environmental Testing Solutions, Inc.

USEPA Control Limits (± 2 Standard Deviations)

-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"-"

-"-"-"-"-"-"-"- ._ .. - .. _ .._ .. - .. _ .._.. _ .._ .._ .._.._.. _ .. _ .._.. _.,

. .. .

USEPA Warning and Control Limits (75th

and 90th

Percentile CVs)

Laboratory Warning and Control Limits (10th and 25 th Percentile CVs)

- .. _.. ..- .._.._.._ .._ .. _ .. - .. _. _.. _ .. _ .. _.. .._.._ .. - .. - ._.._ .._.. - ..

........................................................................................................

.....................

........................................................................................................

........................................................................................................

........................................................................................................

........................................................................................................

0.9

0.8

0.7

0.6

0.5

1.4

- 1.2U~ 1.0.....'Do- 0.8

UlN

U

> 0.6III

"'CI 0.4....

1.4

1.2

1.0

0.8

0.6

0.4

Test date

Graphs senerated from associated excel spreadsheet.

Excel spreadsheet ent ad by; J. Sumner

Reviewed by;

• 7-day IC25 = 25% inhibition concentration. An estimation of the concentration of potassium chloride

that would cause a 25% reduction in Pimephales growth for the test population.Central Tendency (mean IC2s)

Warning Limits (mean IC25 ± SA.10 or SA.7S)

Control Limits (mean IC25 ± SA.25' SA.90- or 2 Standard Deviations)

of 110

~E· T·.. S.• •• •••• ••••••••••~ "."..;'"!

.~. ITesting Solutions, 'no.\SJ Envlronmenta


State and USEPA Laboratory Laboratory USEPA USEPA

Test number Test date 7-dayl~ CT S Control LImits S.. Wilming Umlts SA.2S Control Umlts SA.1S Warning Limits s.~ Control Umits CV

(giL KCI) (gIL KClj CT-2S 0+25 CT-SA.lD cr +54.10 CT -S,Q,.2S CT+SA.Z5 CT - SA.75 CT +SA.75 cr -SA-90 CT+SA.9D

1 03-29-11 0.75 0.72 0.04 0.64 0.80 0.09 0.63 0.81 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.052 03-31-11 0.75 0.72 0.04 0.64 0.80 0.09 0.63 O.SO 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.053 05-03-11 0.78 0.72 0.04 0.64 0.80 0.09 0.63 O.SO 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.05

• 06-07-11 0.76 0.72 0.04 0.64 0.80 0.09 0.63 0.81 0.15 0.57 0.87 0.27 0.45 0.99 0.32 0.40 1.04 0.055 06-14-11 0.63 0.71 0.04 0.63 0.80 0.09 0.63 O.SO 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.066 07-12-11 0.67 0.71 0.04 0.63 0.80 0.09 0.63 O.SO 0.15 0.56 0.87 0.27 0.44 0.99 0.32 0.39 1.04 0.06

7 07-19-11 0.63 0.71 0.05 0.62 O.SO 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.068 08-09-11 0.67 0.71 0.05 0.62 O.SO 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.079 09-13-11 0.66 0.71 0.05 0.61 O.SO 0.08 0.62 0.79 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.07

10 10-04-11 0.73 0.71 0.05 0.61 0.81 0.09 0.62 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.0711 10-11-11 0.76 0.71 0.05 0.62 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.03 0.0712 10-18-11 0.67 0.71 0.05 0.61 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.0713 11-08-11 0.78 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.071. 12-06-11 0.77 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.0715 02-07-12 0.77 0.71 0.05 0.61 0.81 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.0716 02-14-12 0.74 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.99 0.32 0.39 1.04 0.0717 03-06-12 0.64 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.0718 03-06-12 0.68 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.0719 04-12-12 0.71 0.71 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.56 0.86 0.27 0.44 0.98 0.32 0.39 1.03 0.0720 05-08-12 0.77 0.72 0.05 0.61 0.82 0.09 0.63 0.80 0.15 0.57 0.137 0.27 0.44 0.99 0.32 0.39 1.04 0.08

Nate: 7-d ICzs = 7-day 25% inh'lbition concentraflon. An esflmation of the concentration of potassium chloride that would cause a 25% reducflon'ln Pimephales growth forthe test population.

CT = central tendency (mean IC2s).

S = Standard deviation of the lG.ls values.

laboratory Control and Warning LimitsLaboratory control and warning limits were established using the standard deviation of the IC25 values corresponding to the 10th and 25th percentile OJs. These ranges are more stringent than the control and warning limits

recommended by USEPA for the test method and endpoint.

SA.1O = Standard deviation corresponding to the 1f!' percentile CV. (SA.lO = 0.12)

Sus = Standard deviation corresponding to the 2.'1' percentile OJ. (SA.25 = 0.21)

USEPA Control and Warning Urnlu

SA.1S = Standard deviation corresponding to the 75th

percentile OJ. (SA.1S = 0.38)

SA.90= Standard deviation corresponding to the 9dh percentile OJ. (SA.90 = 0.45)

CV = Coefficient of variation of the lG:s values.

USEPA. 2000. Undemanding and Accauntingfor Method Variability In Whole EffluentToxicity Applications Under the National Pollutant DIscharge Elimination Program. EPA-833-R-Q0-Q03. US Environmental Protection Agency, Cincinnati, OH.

File: ppkclcr_050812.xlsxEntered by: J. SumnerReviewed by: --df-

of 110

•••~

o~ Environmental Testing Solutions, Inc.


Precision of Endpoint MeasurementsOrganism Source: Aquatox, Inc..

.......................... . ......

....................................................................•.............................

~---::--

USEPA Acceptance Criteria (> 0.25 mg per surviving larvae)

20

30--------------------------Kentucky Acceptance Limit « 30.0%)

- -- -- -,....----

.....................................................

............. ....................................................................................o

10

~-l: .c0 ..... ;:lQ 0.;:: ..~

Cl-"60 ........ l:l: 0GI U·u ..e .eGI0

U

30USEPA Upper PMSO Bound (90'h percentile < 30.0%)

.....................................................................................

--.............

20

10 .. ................................................................................................

• Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference(PMSD) PMSD is the minimum significant difference between the control and treatment that can bedeclared statistically significant.

Central Tendency (mean Control Growth, CV, or PMSD)

Control Limits (mean Control Growth, CV, or PMSD ± 2 Standard Deviations)

Graphs senerated from associated excel spreadsheet.EKcel spreadsheetellte~ J. Sumner

Reviewed by: -----w~~

of 110

~ETSj EnvlronmentalTestlng Solutlons,lnc.

Precision of Endpoint Measurements

Pimephales promelasChronic Reference Toxicant Data

Test number Test dateControl Control Mean

CT CV CT MSD PMSD CTSurvival Growth

1%) (mg/larvae)for Control Growth

(%)for Control

(%) for PMSD (%)(mgJlarvae) Growth CV (%)

1 03-29-11 100 0.829 6.3 0.09 10.32 03-31-11 100 0.675 0.752 3.2 4.8 0.09 12.8 11.53 05-03-11 100 0.867 0.790 6.3 5.3 0.08 8.8 10.64 06-07-11 97.5 0.839 0.802 5.8 5.4 0.11 12.7 11.1

5 06-14-11 100 0.758 0.793 14.5 7.2 0.13 16.8 12.36 07-12-11 97.5 0.872 0.806 6.0 7.0 0.09 9.8 11.97 07-19-11 100 0.839 0.811 5.4 6.8 0.06 7.2 11.28 08-09-11 100 0.662 0.792 6.6 6.7 0.07 10.6 11.19 09-13-11 100 0.848 0.799 6.7 6.7 0.12 13.6 11.410 10-04-11 100 0.768 0.796 16.8 7.7 0.13 16.9 11.911 10-11-11 100 0.681 0.785 4.3 7.4 0.07 9.9 11.812 10-18-11 100 0.693 0.777 4.8 7.2 0.07 10.0 11.613 11-08-11 100 0.792 0.779 10.3 7.5 0.12 15.6 11.914 12-06-11 100 0.738 0.776 15.7 8.0 0.12 15.9 12.215 02-07-12 100 0.662 0.768 10.5 8.2 0.09 13.4 12.316 02-14-12 97.5 0.658 0.761 5.2 8.0 0.07 10.1 12.117 03-06-12 100 0.854 0.767 3.5 7.8 0.09 10.8 12.118 03-06-12 100 0.873 0.773 5.1 7.6 0.10 11.4 12.019 04-12-12 97.5 0.706 0.769 7.0 7.6 0.06 8.7 11.820 05-08-12 100 0.613 0.761 8.1 7.6 0.08 13.1 11.9

Note: CV = Coefficient of variation for control growth.

Lower CV bound determined by USEPA (10th

percentile) '" 3.5%.

UpperCV bound determined by USEPA (90th

percentile) =20%

MSD = Minimum Significant Difference

PMSD = Percent Minimum Significant Difference

PMSD is a measure attest precision. The PMSD is the minimum percent difference between the control and treatment that can bedeclared statistically significant in a whole effluent toxicity test.

lower PMSD bound determined by USEPA (10th percentile) '" 12%.

Upper PMSD bound determined by USEPA (90th percentile) '" 30%.

cr '" Central Tendancy (mean Control Growth, CV, or PMSDj

USEPA. 2000. Understanding and Accounting for Method Variability In Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program.EPA·833-R-OO-o03. US Environmental Protection Agency, Cincinnati, OH.USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2Appendix. EPA·821·B·01·004 and EPA-821-B-Ol-00S. US Environmental Protection Agency, Cincinnati, OH.

I.d'pend.",Ral'fowbV

Kell'v'·Koen,"

\~I~

File: ppkclcr_050812.xlsxEntered by: 1. Su~rReviewed by:---------19'

of 110

"ET"ell 'w· I§) ,$:'1~r:;':¢ if;;; ~:

~ Envlranmental Te$ting Soilltions, Inc.

Page I of5

PpKCICR Test Number: 1.-1~

Potassium Chloride Chronic Reference Toxicant Test(EPA-821-R-02-013 Method 1000.0)

Species: Pimephales promelas

SOP AT21 - Exhibit AT21.1, revision 06-0 I-II

MHSWbatch used

Anal stTime

Test initiation, renewal,or termination

Anal st

Acceptance criteria

TimeAnal stTime

Morning feeding Afternoon feeding


Avera e wei t er initiallarvae:Average weight per surviving larvae:

% Mortali :Control in ormation:

Day Date

0OS-O~' !"t.

~. • ,"1-2

0$-/0'"3 OS·I\·I'Z..

OS·I"l..·I'l..5

O~'I

6 OS·, -I'L.7 OS'l -11.

Dilution oreoaration information: Comments:KCl Stock INSS number: IIIlS<' 10'l1c.Stock preparation: 50 g KCIIL:

Dissolve 50 g KC! in l-L Milli·Q water.Dilution prep (mgIL) 450 600 750 900 1050Stock volume (mL) 9 12 15 18 21Diluent volume (mL) 991 988 985 .982 979Total volume (mL) 1000 1000 1000 1000 1000

Test oreanism information: Test information:Organism age: 7n.l. L\A.wtC. t" b Randomizing template: b~tJDate and times organisms ~.111' \"'L '\coCO Incubator number and

~'t>were born between: shelf location:Organism source: AThlC. 6A1\.~\ Pf O...·(T\· '"L Artemia CHM number: (MM IoS'1-

Drying informationfor weightdetermination:

Transfer vessel pH- 1.''"' S.u. Date / Time in oven: 0" 'S'll 1Il/0information: Temperature ~ 1.'1.& 'c Initial oven temperature: tao ·tAverage transfer volume:

O. \"to'\.....1lDate / Time out of oven: .,;.. , ... , l.. 11'10Final oven temperature: Ian' (Total drying time: "lJ.!.l1n.IoI(

Ind,p..,don,n....rewbv

J(oll.ve.~ ••n.n

v:-

II

II

II

IIII

~

II

of 110

Day Control 450 m. KCIIL 600 mt KCIILA a C D E F G H I J K L

010 1O 10 It) 10 10 10 10 If) 10 10 10

110 10 10 10 10 fa 10 10 /0 10 10fO

2It) '0 10 1O 10 '0 10 to /0 10 10 /0

310 If) 10 10 10 10 fO 10 /0 10 10If:>

4IC) to Itl It) 10 10 10 10 1010 10 10

5Ie> If) II) /0 10 '0 I() It) 10 10 '0If:>

6 /D If) I() 10 10 JO 10 10 ID fO to107 It> /0 10 10 If) It) ID Ie '0 10 16 10

A Pan weight (mg) (I. Tray color code:: I)....... q}"y,\,.,,!un\Analyst: Mitt 14.1,8 1'5.Se. I~. \'5 13.1t 1'!>.'l4 It.'1s 13.'51 14.4-1 1!>."I'Z. IS.I1- I!>. 1.1.- 13.'S1J)ate: 0'5.04.12

B - Pan + Larvae weight (mg)Aoalyst: fl1):r

~.36 11,01- /1.31 IQ.1f 20,10 2D./Q ''l.~f, ZD:SJ ~O.lfe l.I.sc, let.'¥f Z/).'v'z.Date: 06:i'tj1C - Larvae weight (mg) - B - A

Hand calculated. 4 ...10 'S...., r.:ZI.. lo.n. (,.~, '.a'l S."I1 S.f'I r. .l>Io 10.1'\ 1...11 7./1Analyst: • e

Weight per initial number oflarvae (mg)= C I Initial number of larvae

\D~ ~ ~ 4-~ ~\D ~JIo,<:l~ ,if "to \.a.- t-Hand .calculated. OJ \II &ii 'Ii C; " \II \0 1'\

Analyst: ~ C' 0 b' 0' 0' ~. ~. I)' 0' O' O' ()'

Average weight per· initial Percent ,number of larvae (mg) reduction

O.I.I~ O.lo~~ -~.~7, o.",~1 -10,'2.7,from control(%1

Comments:

PpKCICR Test Number:~




Comment codes: c = clear, d = dead, fg = fungus, k = killed, m =missing, sk ~ sick, sm =unusually small,Ig ~ unusually large, d&r = decanted and returned, w ~ wounded.

I"d.pond'.'ROVIewby

~.II·VE.~ ••n.n

b

IIIIIIIIIIIIII'"

III"

I ~

Ii

of 110

Comment codes: c =clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small,19 = unusually large, d&r = decanted and returned, w =wounded.

Day 750m KCVL 900m KCVL 1050 m KCVL

M N 0 p Q R, S T U V W X0

It> 10 Ie 10 10 10 10 10 1010 10 101 8~ ."- ,J. .(, 'l~ .'\ct '/$"14,. , ?:' -S"" :..$01,. '"'" ~'\ I:l '\ to (., 5 SO'"~2 g q q '\ 10 t., g ~'o\ ?:JIIJ... ?:J1.G\. ~'1~ ~t<\

3& &'A 1\' q sill. S'<t ,'.{ 5 '2'0\ ' "I- -?> ~"2-

4 g ~ '\ t:J... 5 I ,{'\ 'S S 1.. .3 -3

5~ 'I '\ L\ U(. ~ ~ .3 \'loig fc:, \ 1-

6 g'" " v\ -z... '~& 8 '\ "\ ':> ~ S \ \7

& ~Io\ 1'" 4Uo s \.", \ I~ \\,,&0& S '5 \ ....

A - Pan weight (mg) (Tray color code:: OAtIL!:J,,,,, bm4r0Analyst: 1\1"* IS.lI'! 1'3.~O 1'3.14- 1t..:}O 14.'-Z 13:5z 14.0'; 14.0$ 13."l1 13.44- 14.B'I II}. t.'lDate: QS·p4.."z.

B - Pan + Larvae weight (mg)

Analyst:~~ ZM/z. 18.'111 '6."Il rM.El '8.01{ IlA'02- 11-:03 11-.~\Q I~.\f"l /'I.tel 1'5.1QO 15.1J,Date: ~l2L

C - Larvae weight (mg) - B - A

Hand calculated. <9} . s.~ If.'11. 'l.'~ ".5& !."l'2. 3.S0 3.1~ ~..'11 0.1& O.t"4 0.1\ ~.11..Analyst: ",-

Weight per initial number oflarvae (mg)= C I Initial number oflarvae

~ ',,~ .~ ~ ~~ ~ t",l!1 ~ ~ ~~ ~\)Hand calculated.

..\"1.-- '!J 0'[:1 ~,)'; .:;.. '" Ilj ()'Analyst: 0' ~. O' O' O· ~. t>' 1;)' C' C'Average weight per'"initial Percentnumber of larvae (mg) reduction 0.,\,\-5 It\. '11. 0.1105 &.Io.'H 0, o~1 8r..11.

from control(%)

rIk'

Wi

_1l'

IIIIIIIII

-III'I,

t",,\~,.

°ETS°" III ' ,® IA'i;~,?! i~ '~'f:::",{('f;'~ Enviranm~nlaITe5t1ng Sallltians, rn~.


Comments:

In~.p..,d"'l

RovIowbVK.IIO'f~,K••n.n

~

Page 3 of5



SOP ATIl - Exhibit ATILl, revision 06-01-11

of 110

@jD~~fti,,~ a~I~~

Pimephales promelas Chronic Reference Toxicant TestEPA-821-R-02-013, Method 1000.0

248

May 08-15, 2012

Test number:------Test dates: ---------=-----"--


ConCllntl'lltlon (mc/L KCI RepllCllte Inltlel number of Rnel number of A = Pen weflht (mil B=Pen+"1'VIItI "Mle weflht (mil = Wellht / Survlvlnl Meen wellht/SUrvlvlnl Coelllcfent of VIlrfetlon Weflht / fnltlel number of Meen survlVllI ("I Mun wellhtf Inltlel Coefficient of VIlrfetlon PerCllnt reduction fromIIIM1e lerwe weflht(mll A-B number of lerVll8 (mil number of leMle (mil IMellft.,..htper.urAvInI 'e_e(m,l number of larYlle ("I control ("I

.umbo. of 10...1("I (mil

A 10 10 14.68 21.38 6.70 0.670 0.670

Control B 10 10 13.56 19.07 5.51 0.5510.613 8.1 0.551 100.0 0.613 8.1 Not applicable

C 10 10 13.13 19.39 6.26 0.626 0.626D 10 10 13.72 19.75 6.03 0.603 0.603E 10 10 13.79 20.10 6.31 0.631 0.631

450 F 10 10 12.95 20.14 7.19 0.719 0.633 9.60.719

100.0 0.633 9.6 -3.3G 10 10 13.39 19.36 5.97 0.597 0.597H 10 10 14.47 20.31 5.84 0.584 0.584I 10 10 13.92 20.48 6.56 0.656 0.656

600J 10 10 15.17 21.36 6.19 0.619

0.651 6.80.619

100.0 0.651 6.8 -6.2K 10 10 13.62 19.79 6.17 0.617 0.617L 10 10 13.51 20.62 7.11 0.711 0.711

M 10 8 15.29 20.82 5.53 0.691 0.553

750N 10 8 13.50 18.46 4.96 0.620

0.639 6.80.496

77.5 0.495 8.4 19.20 10 8 13.74 18.47 4.73 0.591 0.473P 10 7 12.70 17.28 4.58 0.654 0.458Q 10 4 14.62 18.04 3.42 0.855 0.342

900 R 10 5 13.32 16.82 3.50 0.7000.773 8.3

0.35047.5 0.365 6.3 40.4

5 10 5 14.05 17.83 3.78 0.756 0.378T 10 5 14.05 17.96 3.91 0.782 0.391U 10 1 13.91 14.64 0.73 0.730 0.073

1050 V 10 1 13.44 14.28 0.84 0.8400.813 14.7

0.084-10.0 0.081 14.7 86.7

W 10 1 14.89 15.60 0.71 0.710 0.071X 10 1 14.29 15.26 0.97 0.970 0.097

Dunnett's MSD value:PMSD:

O.OBOO13.1

MSD=PMSD=

Minimum Significant DifferencePercent Minimum Significant Difference

PMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatmentthat can be declared statistically significant in a whole effluent toxicity test.Lower PMSD bound determined by USEPA (10th percentile) = 12%.Upper PMSD bound determined by USEPA (90th percentile) = 30%.

Lower and upper PMSD bounds were determined from the 10th and 90th percentile, respectively, of PMSD data from EPA's WET Interlaboratory Variability Study (USEPA, 2001a; USEPA, 2001b).

USEPA. 2001a, 2001b. Final Report: Interlaboratory Variability Study of EPA Short-term Chronic and Acute Whole Effluent TOXicity Test Methods, Volumes 1 and 2-Appendix. EPA-821-B-Ql-004 and EPA-821-B-Ql-Q05. US Environmental Protection Agency, Cincinnati, OH.

File: ppkclcr_050812.x1sxEntered by: J. S'f)ferReviewed by: -et-

of 110

1 2 3 41.0000 1.0000 1.0000 1.00001.0000 1.0000 1.0000 1.00001.0000 1.0000 1.0000 1.00000.8000 0.8000 0.8000 0.70000.4000 0.5000 0.5000 0.50000.1000 0.1000 0.1000 0.1000

~~TS~ Environmental Testing Solution" Inc.

Start Date: 5/812012End Date: 5/15/2012Sample Date:Comments:Cone-mall

D-Control450600750900

1050


Larval Fish Growth and Survival Test-7 Day SurvivalTest ID: PpKCICR Sample 10: REF-Ref ToxicantLab 10: ETS-Envir. Testing Sol. Sample Type: KCL-Potassium chlorideProtocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas

Transform: Arcsin Square Root Rank 1-TailedCone-mg/l Mean N-Mean Mean Min Max CV% N Sum Critical

D-Control 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4450 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00600 1.0000 1.0000 1.4120 1.4120 1.4120 0.000 4 18.00 10.00

'750 0.7750 0.7750 1.0782 0.9912 1.1071 5.379 4 10.00 10.00*900 0.4750 0.4750 0.7602 0.6847 0.7854 6.622 4 10.00 10.00

*1050 0.1000 0.1000 0.3218 0.3218 0.3218 0.000 4 10.00 10.00

Auxiliary TestsShapiro-Wilk's Test indicates non-normal distribution (p <: 0.01)Equality of variance cannot be confirmedHypothesis Test (1-tail, 0.05) NOEC LOEC ChV TUSteel's Many-One Rank Test 600 750 670.82Treatments vs D-Control

Statistic0.63088

Critical0.884

Number TotalReap Number

0 400 400 409 40

21 4036 40

Skew Kurt-2.1517 5.4494

Ind.p..d,otRO'I1..... tIy

t<elleye.Koona"

~.~:

Maximum Ukellhood-ProbltParameter Value SE 95% Fiducial Limits Control Chi...sg Critical P-value Mu Sigma IterSlope 14.4814 1.97334 10.6137 18.3492 0 1.78643 7.81472 0.61789 2.93943 0.06905 3Intercept -37.567 5.80045 -48.936 -26.198TSCR 1.0Point Problts mg/l 95% Fiducial limits

0.9EC01 2.674 600.876 521.365 654.429EC05 3.355 669.645 602.697 714.916 0.•EC10 3.718 709.467 650.476 750.155 0.7EC15 3.964 737.664 684.352 775.454EC20 4.158 760.871 712.091 796.659 BI 0.6

0EC25 4.326 781.362 736.336 815.805 8. 0.5EC40 4.747 835.476 798.142 869.388

~ 0.4EC50 5.000 869.818 834.745 906.603EC60 5.253 905.573 870.247 948.43 0.3EC75 5.674 968.289 927.107 1028.37 0.2EC80 5.842 994.365 949.312 1063.47EC85 6.036 1025.65 975.202 1106.65 0.1

EC90 6.282 1068.41 1008.03 1164.36 0.0EC95 6.645 1129.83 1057.65 1256.74 1 10 100 1000 10000EC99 7.326 1259.13 1155.35 1452.86 Dosemg/l

Dose-Response Plot

0.9

0.•

~ 0.7

'E 0.6

•tn 0.5

B0'...0.3

0.2

0.1

0 :g 0 0 0 0 0~ 0 ~ 0 ~

0 .. w ~ ~ 0

8 rII

File: ppkclcr_050812.x1sxEntered by: 1. SrrReviewed by:~

of 110

~~TS,.) Environmental TOlSII"IISolldlon"lnc.


1 2 3 40.6700 0.5510 0.6260 0.60300.6310 0.7190 0.5970 0.58400.6560 0.6190 0.6170 0.71100.5530 0.4960 0.4730 0.45800.3420 0.3500 0.3780 0.39100.0730 0.0840 0.0710 0.0970

Start Date:End Date:Sample Date:Comments:Conc-mg/l

D-Control450500750900

1050

5/8/20125/15/2012

larval Fish Growth and Survival Test-7 Day GrowthTest 10: PpKCICR Sample 10: REF-Ref ToxicantLab 10: ETS-Envlr. Testing Sol. Sample Type: KCL-Potassium chlorideProtocol: FWCHR-EPA-821-R-02-013 Test Species: PP-Pimephales promelas

Transform:- Untransformed i-Tailed IsotonicConc.mg/l Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean

D-Control 0.6125 1.0000 0.6125 0.5510 0.6700 8.087 4 0.6320 1.0000450 0.6328 1.0331 0.6328 0.5840 0.7190 9.612 4 -0.552 2.180 0.0800 0.6320 1.0000600 0.6508 1.0624 0.6508 0.6170 0.7110 6.760 4 -1.042 2.180 0.0800 0.6320 1.0000750 0.4950 0.8082 0.4950 0.4580 0.5530 8.425 4 0.4950 0.7832900 0.3653 0.5963 0.3653 0.3420 0.3910 6.320 4 0.3653 0.5779

1050 0.0813. 0.1327 0.0813 0.0710 0.0970 14.714 4 0.0813 0.1286

Auxiliary Tests Statistic Critical Skew KurtShapiro-Wllk's Test indicates normal distribution (p > 0.01) 0.92935 0.805 0.61878 -0.6846Bartlett's Test indicates equal variances (p = 0.87) 0.28594 9.21035Hypothesis Test (i·tail. 0.05) NOEC LOEC ChV TU MSOu MSOp Msa MS. F-Prob dfDunnett's Test 600 >600 0.08004 0.13067 0.00146 0.0027 0.59872 2, 9Treatments vs D-Control

linear Interpolation (200 Resamples)

1500500 1000

Dose mg/l

1.0,-----------,0.•

0.8

0.7

0 .•

al 0.5! 0.4~ 0.3

0.2

0.1 ••

0.0 ~-."~""'""~_.-." ..-0.1-0.2 +-._~~__~~_-.-I

o

Point mg/l SD 95% CL(Exp) SkewIC05 634.60 28.01 593.31 661.72 -7.8928IC10 669.20 14.86 633.19 723.43 0.9209ICi5 703.80 19.42 666.12 781.73 0.9450IC20 738.39 20.46 694.18 806.57 0.4813IC25 774.28 20.26 718.07 838.43 0.1632IC40 883.87 12.92 841.40 918.61 -0.1823IC50 926.01 5.72 908.42 943.16 -0.0795

Dose-Response Plot

0.8,-------~----_-------_,

0.7

0.8

l05o.., 0.4

iI'~ 0.3

0.2

_ • __ • • 1-tall,0.05Ievelof significance

0.1

0g 0 0 0 0 0

~ 0 ~ 0 ~

" ~ ~ ~ Q~0

"cInd,p.ndent

R..I b~

KelloyE.X n

'"tt-File: ppkclcf_050812.x1sK

Entered by: J. SlIJIUIerReviewed by:~

of 110

iIIIIIIIII

Ii

IIi

II

Ii

I~~,

tnd0f>l'odo"ROIllowbV

KeliovE.Ke<n'n

E


Concentration

CONTROL ,,~ .

450 mgKCllL

600 mg KCVL IMO

1.0.1. '"

750 mg KCIIL 1(,301.1.(."I

900 mg KCIIL

1050 mg KCIIL

STOCK

Initial

Page 4 of5

PpKCICR Test Number: t"'&

SOP AT2 I - Exhibit AT2 I. I. revision 06-0 I-II

of 110

FinalInitialFinal


Page 5 of5

Initial

SOP AT21 - Exhibit AT2I.l, revision 06-01-11

Final

81

eQI

\050

'1.'-\·1

~,,(li

1."nlo

'1'-\ .S:

8.00

V>I':)\.iIO

1.....&i~H)O

1.4

l 'tl '60

l.LI.(8001.'-1

?OSO'1.... JI 1.~.,\

Initial Final Initial

Anal st

CONTROL "'~


"ETS..,~ i: ...~, !. ,; ';~

0.•'~....~... i1....•,'.' ~~.•..c"':) ;~;!.:,": ~r: ~iJ,~

~ EnvironmenialTI!5ting Solutions, Inc.

Concentration Parameter

600 mg KCl/L

750 mg KCI/L

900 mg KCI/L

450 mg KCI/L

1050 mg KCI/L

l,depOJ1doolROIII..... bV

KelloyE.K..n.n

"P=-

IIIIIIIIIi

I

~IIIIII

of 110

••• Ceriodaphnia dubiaChronic Reference Toxicant Control Chart

Environmental Testing Solutions, Inc.

...............................................................................................

USEPA Control Limits (± 2 Standard Deviations)

. ........ -.,.,..--'. ----- .••

~--

. .

----~t--- -----

Laboratory Warning and Control Limits (10th and 25th Percentile CVs)

USEPA Warning and Control Limits (75th and 90th Percentile CVs)

_ .._.. _ .._.. _.

... .......... . . ...........................................

......... ...•...............

...............................................................................................

...............................................................................................

...............................................................................................

• • • • • • • • • • • • • • • • • • • •

1.14

1.12

1.10

1.08

1.06

1.04

1.02

2.5QU 2.0~

Z~ 1.5~'-'

."...1.0U....

~~ 0.5"0I

t--

1.4

1.3

1.2

1.1

1.0

0.9

Test date

• 7-day IC25 = 25% inhibition concentration. An estimation of the conceptration of sodium chloride

that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.

- -. Central Tendency (mean IC2s)

_._.- Warning Limits (mean IC2s ± SA.1O or SA.7S)

•••..••.• Control Limits (mean IC2s ± SA2S' SA.90' or 2 Standard Deviations)

GraphSllenerated from associated excel spreadsheet.

Excel spreadsheet entered by: J. Sumner

Reviewed by:~

of 110

rrt if~t

••ili!l®o'~~TSEnvironmental Testing Solutions, Inc.

Ceriodaphnia dubiaChronic Reference Toxicant Control Chart

State and USEPA Laboratory Laboratory USEPA USEPA

Test number Test date 7-day IC25 CT S Control Limits S'M Warning Limits S.~ Control Li mits S'H Warning Limits SA.')O Control Limits CV

(giL NaCI) (giL NaCl) CT-2S CT+2S cr -SA,lD cr +SA.to CT-SA.2S CT+ SA.25 CT - SA.75 CT+ SA.75 cr - SA.90 CT+SA.9o

1 01-18-11 1.06 1.07 0.01 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.56 0.66 0.41 1.74 0.012 02-08-11 lD' lD1 0.01 LOS 1.09 0.09 0" 1.16 0.18 0.89 125 OAS 0.59 155 0.66 0.41 1.74 ODl

3 03-08-11 LOS 1.07 am 1.05 1.10 0.09 0.99 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.74 0.014 ~5-11 1.06 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.Q1

5 04-05-11 1.07 1.07 0.01 LOS 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01, 05-03-11 1.07 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.017 06-07-11 1.05 1.07 0.01 1.05 1.09 0.09 0.98 1.16 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01

8 07-12-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.01

• 08-09-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 p.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.0110 09-13-11 1.06 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.0111 10-04-11 1.07 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.89 1.25 0.48 0.59 1.55 0.66 0.41 1.73 0.0112 10-11-11 1.06 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.0113 11-08-11 1.05 1.07 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.73 0.0114 12-06-11 1.06 1.06 0.01 1.04 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.0115 01-10-12 lDB 1.07 0.01 1M 1.09 0.09 0.98 1.15 0.18 0118 1.25 OA8 OS. 154 0.66 DAD 1.73 DDt16 02-07-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.0117 03-13-12 1.08 1.07 0.01 1.04 1.09 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.55 0.66 0.40 1.73 0.0118 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.25 0.48 0.59 1.54 0.66 0.40 1.72 0.01

" 04-10-12 1.07 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.0120 05-mH2 1.06 1.06 0.01 1.05 1.08 0.09 0.98 1.15 0.18 0.88 1.24 0.48 0.59 1.54 0.66 0.40 1.72 0.01

Note: 7-d IC2S '" 7-day 25% inhibition concentration. An estimation of the concentration of sodium chloride that would cause a 25% reduction in Ceriodaphnia reproduction for the test population.

CT '" Central tendency (mean IC2s).

S'" Standard deviation of the I~ values.

Laboratory Control and Warning LimitsLaboratory control and warning limits were established using the standard deviation of the IC2S values corresponding to the 10th and 25th percentile CVs. These ranges are more stringent than the control and

warning limits recommended by U5EPA for the test method and endpoint.

Suo'" Standard deviation corresponding to the 10th percentile CV. (5A.1O '" 0.08)

SA.2S '" Standard deviation corresponding to the 25th percentile CV. (SA,25 '" 0.17)

USEPA Control and Warning Limits

SA.7S'" Standard deviation corresponding to the 75t~ percentile CV. (SA.7S'" OAs)

SA.9II'" Standard deviation corresponding to the 90th percentile CV. (SA.9Q '" 0.62)

CV '" Coefficient of variation of the lC:!s values.

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant Discharge Elimination Program. EPA-833-R-OO-003. US Environmental Protection Agency, Oncinnati, OH.

File: CdNaCICR_050812.xlsxEntered by: JJlnerReviewed by:

of 110

~ETSj Environmental Testing Solutions. Inc.

Ceriodaphnia dubiaChronic Reference Toxicant Control Chart


40

Minimum Acceptance Criteria (> 15.0 offspring per snrviving female)

...............................................................--

...... . .............. .......................•...-----....,-

= 350.. 'Q;'....Col -= ~ 30"Cl ao~.. -.Q.i)J) 25.. =~ ....- Q.0 20.. =....= 00 '-'U 15

Kentncky Acceptance Limit « 30.0%)

North Carolina Acceptance Limit « 40.0%)

30

......................... . .10

20

40

40USEPA Upper PMSD Bonnd (90th percentile < 47.0%)

~f 20

10 .............. . .

• Control Reproduction, Coefficient of Variation (CV), or Percent Minimum Significant Difference(PMSD) PMSD is the minimum significant difference between the control and treatment that can bedeclared statistically significant.

Central Tendency (mean Control Reproduction, CV, or PMSD)

Control Limits (mean Control Reproduction, CV, or PMSD ± 2 Standard Deviations)

Graphs generated from associated eKcel spreadsheet.

EKcel spreadsheet e:.~ed by: J. Sumner

Reviewed by:~

of 110

•••#I''''.'.'.®

()

j Environmental Testing Solutions, Inc.


Ceriodaphnia dubiaChronic Reference Toxicant Data

TestTest date

Control Control Mean

number SUNival Reproductioncr CV cr MSD PMSD cr

for Control Mean for Control(%) (offspring/female) Reproduction (%) Reproduction (%) for PMSD (%)

(offspring/female) CV(%)

1 01-18-11 100 31.9 4,8 2,0 6.32 02-08-11 100 30.7 31.3 6.3 5.6 2.3 7.4 6.93 03-08-11 100 32.6 31.7 6.9 6.0 1.8 5.4 6.44 04-05-11 100 32.8 32.0 4.8 5.7 2.0 6.0 6.35 04-05-11 100 33.0 32.2 5.9 5.8 2.2 6.7 6.46 05-03-11 100 30.9 32.0 4.5 5.5 1.9 6.1 6.37 06-07-11 100 28.9 31.5 6.7 5.7 2.5 8.7 6.68 07-12-11 100 32.2 31.6 6.0 5.7 2.0 6.2 6.69 08-09-11 100 28.3 31.3 5.0 5.7 1.9 6.8 6.6

10 09-13-11 100 32.1 31.3 8.2 5.9 1.9 5.8 6.511 10-04-11 100 32.8 31.5 6.1 5.9 2.1 6.5 6.512 10-11-11 100 31.3 31.5 3.0 5.7 2.1 6.6 6.513 11-08-11 100 29.0 31.3 7.6 5.8 1.8 6.1 6.514 12-06-11 100 29.3 31.1 5.8 5.8 1.9 6.5 6.515 01-10-12 100 32.4 31.2 5.1 5.8 2.4 7.4 6.616 02-07-12 100 31.7 31.2 4.2 5.7 2.1 6.5 6.617 03-13-12 100 31.1 31.2 7.2 5.8 1.9 6.1 6.518 04-10-12 100 29.4 31.1 6.8 5.8 2.4 8.0 6.619 04-10-12 100 30.3 31.1 7.1 5.9 2.7 8.8 6.720 05-08-12 100 31.7 31.1 6.1 5.9 1.9 6.1 6.7

Note: CV =Coefficient of variation for control reproduction.

lower CV bound determined by U5EPA (10th percentile) =8.9%.

Upper CV bound determined by USEPA (90th percentile) = 42%

MSD =Minimum Significant Difference

PMSD = Percent Minimum Significant DifferencePMSD is a measure oftest precision. The PMSD is the minimum percent difference between the control and treatment thatcan be declared statisticallv significant in a whole effluent toxicity test.

, lower PMSD bound determined by USEPA (loth percentile) =13%'.

Upper PMSD bound determined by USEPA (90th percentile) =47%.CT =Central Tendancy (Mean Control Reproduction, CV, or PMSD)

USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent Toxicity Applications Under the National Pollutant DischargeElimination Program. EPA-833-R-00-003. US Environmental Protection Agency, Cincinnati, OH.

USEPA. 200la, 200lb. Final Report: Interlaboratory Variability Study of EPA ShorHerm Chronic and Acute Whole Effluent Toxicity Test Methods,Volumes 1 and 2-Appendix. EPA-821-B-Ol~004 and EPA-821-B-Ol-00S. US Environmental Protection Agency, Cincinnati, OH.

led.p,nd,n'RovlowOy

~.rlO'fE,Ke.nan

~

File: CdNaCICR_050812.xlsxEntered by: J. SumnerReviewed by:+

of 110

Page lof6

'~l' .

j

~J

J

Sodium Chloride Chronic Reference Toxicant Test(EPA-821-R-02-013 Method 1002.0)

Species: Ceriodaphnia dubia

-1. J

CdNaCICR #: \0'"\

Dilution nrenaration information: Comments:NaCI Stock INSS number: ~IStock preparation: 100 g NaCIIL;

Dissolve 50 g: NaCl in 500 roL Milli-O water.Dilution;;;enCn;<;m 600 800 1000 1200 1400Stock volume (mL) 9 12 15 18 21Diluent volumermL) 1491 1488 1485 1482 1479Total volume (mL) 1500 1500 1500 1500 1500

J

J

Test orllanism source information: Test information:O~anism age: < 24-hours old Randomizing temnlate color:Date and times organisms were born O"'~'I"\- 0l0/oi0 "I1:l 01llO Incubator number and shelfbetween: location: '&'2-Culture board: O\'(l'I"L ,.- .

Replicate number: 1" 3

4 S I 6 7 I 8 I 9 10 YWTbatch:Culture board eu" number: ~ I 4. I. ..~ 11. I,,, \';) I " 11.\ 1.1- oS· 0\-'1..

Transfer vessel infonnation: I nH ... ,tS.U. Temnerature - 1.41;,. t 'c Selenastrum batch:CM·~()·n ..Average transfer volume (mL): O. CP.>'2.1...-lL

Daily renewal information:

Day Date Test initiation and feeding, MHSW Analystrenewal and feeding, or batch used

termination time- 1

0!

oil2

O~ 10- j3 0&0"\4

0&10. 15 DC "'L'~ j ••6

'11-7 as· I" "'1.- o.to'l

- j

• J

Control iiJormation: Acceptance criteria Summarv oftest endooints:% of Male Adults: (\7. :520% 7-day LCso ') 10..(O 0% Adults havinQ 3" Broods: '''....7 >80% NOEC 1000

.% Mortalitv: A1 :520% LOEC \ ., ""'"•Mean OffsoriiNIFemale: .~\ .1 ~ 15.0 offspring/female ChV 1""<' ."%CV: -~ \'1. <40.0% IC" 10"'.... 1.1

I,dep..d,nlRovI..... !Iy

I<elleye.Koon,n

~' SOP ATl4 - Exhibit ATl4.1, revision 06-01-11

of 110

'1J

" J Page 2 of6

Replicate numberDay 1 2 3 4 5 6 7 8 9 10

I Young produced n a a n () D 0 () () (JAdult mortality l- '- L. L.. L.. L.. L L.. L.. L-

2 Young produced (') 0 .() () [) (l a 0 0 ()Adult mortality L L. L.. l- '- L- L.. L.- L.- '--

3 Young produced 0 0 D 0 0 () (') (). 0 ()Adult mortality L L L. L. l- I...- l.- I.- '-- '--

4 Young produced S '-\ '-\ S ~ s 5 '-\ '-l '-\Adult mortality L I.- L. L L. I. '-- L.. L. II.-

5 Young produced \L.... 11- Ie 1\ I~ 1\ Ie \\ 1\ ILAdult mortality L. '- \......... l...- I.- '-- L. \-. \- '--

6 Young produced D 0 0 0 0 D (] 0 0 0Adult mortality L.. L L \.. L I...- L I...- '-- \......

7 Young produced 11 \1 Ie::. 1'1" IS la IS Ii lie> IITotal young produced :!l'-\ 33 '2.,,\ 1.'\ 31 !I~ ~b <33 3\ 33Final Adult Mortality L. l- l- \.... I..... L. '- I...- ... \........X for 3 rd Broods X )<- "'- }<. ')(:.. ><. )<. 'l'C )<.. 'Ie::

"1j

']'1,

J

Species: Ceriodaphnia dubiaCONTROL

CdNaClCR#:


I~

Note: Adult mortality (L - hve, D = dead), SB - spltt brood (smgle brood split between two days), CO - carry over (offsprmgcarried over with adult during transfer).

DdS . I dRNCIIL600

Concentration:% Mortality: I D1.Mean Offspring/female: I ,3\.1

ID2 a urvlva an epro uctton ataReulicate number

Day 1 2 3 4 5 6 7 8 9 10

1 Young produced f"\ 0 0 (') () () n 0 () (")Adult mortality L L L. L L L L. L L L

2 Young produced U n n 0 () () 0 0 () 0Adult mortality L '- L. L. L L. L L L L.

3 Young produced 0 0 () C) 0 C () 0 a nAdult mortality L L L I..... l.- I...... L L. L ~

4 Young produced u,. "\ S '-\ '-\ 5 '"\ '"\ '" "-IAdult mortality \.- I.- '- L. I...- L' ..l... I...- L L

5 Young produced' \1.. I"L lD It> 10 11- II ID \1- /IAdult mortality '\.... '-- '-- I...... I..... . I..... \-. I...... '-- '--

6 Young produced () 0 0 0 0 0 0 0 0 0Adult mortality L L L \.. L L. L L L I...-

7 Young produced 1'1 11.0 I' IS 18 Ii Ii II. II" .'\Total young produced 03 3"2.. ,be> 1.."1 31. 3"\ a~ 3() 3~ 30Final Adult Mortality l L L. '- '- '-- \- \. ... \-

~ J

- !

~ .J

Note: Adult mortality (L - hve, D - dead), sa - spltt brood (smgle brood split between two days), CO - carry over (offspringcarried over with adult during transfer).

Concentration:% Mortality: 07.Mean Offspring/female: 031 •.,% Reduction from Control: 0.07.

Ind.p,n~.n'

RovlowOyKelleyE,Keen'n

~"SOP ATl4 - Exhibil ATl4.\, revision 06-0\-\\

of 110

Page 3 of6

CdNaCICR#:~

DdS . I dRSpecies: CeriodaDhnia dubia800 N CIILmil a UTVlVa an epro uctzon ata

Replicate number

Dav 1 2 3 4 5 6 7 8 9 101 Young produced () t\ () () () ,...., (") (") 0 0

Adult mortality '- L l L. L '- L L L. '-2 Young produced 0 b 0 (:) () 0 0 a 0 ()

Adult mortality 1..- \... L. L. L. L I,...... L. '- L..3 Young produced (", t> D 0 0 0 .(', 0 {) 0

Adult mortality '-- \- '-- '- L. L L.- '-- L. L..4 Young produced 50 " '-t S So U. '-t .c:. '-\ '--\

Adult mortality L. '-- L. L. '-- \..- I.. \.... L '--5 Young produced 10 \\ ...... \ ..... 1\ 11- ID Itl \"Z.• IC>

Adult mortality L. L. '-- L. '-- L L..... \.... \.... '-6 Young produced 0 D 0 b () D () () 0 0

Adult mortality \... L. '- L. '-- L. L L. L. L.....

7 Young produced I,. II.:. 1"\ I~ \'-' \" \"b \~ 110 I'Total young produced

~ ~'L '2.& ~'1.. ~'2. 3\ "2.1 a~ ~'2... ~\

Final Adult Mortalitv \..... '- \... '-- L. ... '- ... L. '--

°ETS•61 ' ,. - ~

"0 '.".('... i>:. .....:;1,)l':~",.:" ,T" !~'

~ En",onmem,ITostlng50Illllon.. lnc.

1~ ,

~ J

qI

'-'- J

Note. Adult mortahty (L - hve, D dead), sa spltt brood (smgle brood spltt between two days), CO carry over (offsprmgcarried over with adult during transfer).

Concentration:% Mortalitv: 07.Mean OffspringfFemale: 3\. \% Reduction from Conirol: \,"17..

d t' D tS . I dRNCIIL1000 mll: a UTVlva an epro uc zon aaReplicate number

Day. 1 2 3 4 5 6 7 8 9 101 Young produced (') () () f\ I"J a 0 () 0 t'i

Adult mortality l- I,...... L L. L. l- I- I,...... L L..2 Young produced 0 () () {) 0 () f\ 0 (') 0

Adult mortality \..... L I,...... I,...... L.. L \..... L.. L. L3 Young produced 0 0 0 0 0 0 (\ a CJ a

Adult mortality I,...... \ I L. L. L- L \... L L..4 Young produced '-\ '-I. 5:- 4 '-I. ~ \..\ \.{ "'- S

Adult mortality '- I,...... \..... \.... L L. \.... \.... \.... L..5 Young produced \"1.- 10 \\ \"b \\ ID \'1. I"L ID 10

Adult mortality L.. '- L \..... L L. L. L. L L.6 Young produced (') /') (") () 0 (\ [) 0 0 0

Adult mortality \..... \... L. L L. L L.. L \.... L...7 Young produced \"\ \Ia 1'-4 IS I'" 1:'\ I' I'; I¢ tC\

Total young produced3.\ ~ ~C !l'Z, ~\ 1.1 ~~ 3\ 1.& ~O

Final Adult Mortalitv \. \. '- L. L. ....... ~ ~. I..- L..Note: Adult mortality (L - hve, D:::: dead), SB - splIt brood (smgle brood split between two days), CO - carry over (offsprmgcarried over with adult during transfer).

lodepertder>tRolli,wbV

](o1l'V~·KHn."loltl.,~

~

Concentration:% Mortality: 01Mean OffspringfFemale: 30,~% Reduction from Control: 0./. &:1'7.

SOP AT14 - Exhibil ATl4.1, revision 06-01-11

of 110

"ETS•; i~· - I,,; , -~'~"i'.· i ,;J,~ in"","",.nl.llOllingSOIUllo""ro",

Page 4 of6

CdNaCICR #: l~'i.

d t' D t

d t D t

S 'I dR

S 'I dRNCIIL

Species: Ceriodaphnia dubia1200 N CIIL

Note: Adult mortality (L - hve, D - dead), SB - spltt brood (single brood splIt between two days), CO - carry over (offspnngcarried over with adult during transfer).

1400

ml!: a urvlva an epro uc IOn aa

Replicate number

Day 1 2 3 4 5 6 7 8 9 101 Young produced 0 a n 0 5 () () (") 0 ()

Adult mortality L \.... L.. L L- L.. L- ~ L- '-2 Young produced 0 n 0 n 0 0 n 0 0 (')

Adult mortality 'l.- L- L L- L- L L- L L '-3 Young produced 0 0 (') 0 0 () n D () (")

Adult mortality '- \..- l- L L- L L '- '- \.4 Young produced C \ \ "L \ b "" \ \ "L

Adult mortality '- L.. L- '-- '- '-- L- L '- \....5 Young produced '0 c:. "'2.. ~ ~ t> Cl .~ '-I \

Adult mortality L \ L '- '- L- '-- \... L. l6 Young produced 0 D b D (;) S "L C 0 CJ

Adult mortality "- '- '- \... L. L... '-- '- L '--7 Young produced , \Il' "\ I., '" 5 1 "'\ ~ 5

Total young produced '0 1"2. , II .,1,3 \'Z. , & d

Final Adult Mortality l- '- '- '- '- '- '-- \... '-- '--- -

Concentration:% Mortality: fJ7.Mean OffspringJFemale: ,\:1% Reduction from Control: 1A.<.t'.

mil: a urvlVa an epro uc IOn aaReolicate number

Dav 1 2 3 4 5 6 7 8 9 10

1 Young produced () n () () n (') 0 t"'l (') aAdult ~ortality L L L L L L '- '-- L L

2 Young produced ') () .. 0 0 (') (") 0 0 () (')

Adult mortality - L. L L L L L L- I- L-

3 Young produced :J D a 0 0 0 0 0 () ()Adult mortality \.- L L L '- l- I-. L. I- '--

4 Young produced 0 0 \ 0 \ 0 \ \ ("') r....Adult mortality L. L. '-- '-- L '- L... '-- '-- '--

5 Young produced () \ 0 \ 0 7-. (') C .,"2, '-Adult mortality L. L.. L.. I-. '-- L. L \... L \..-

6 Young produced 0 0 f'\ /") rJ (') () () C ()Adult mortality \.... L L L L L \... L. '- '--

7 Young produced 5 ~ 0 ~ 4 I ,":I- 0 0 "'Z...-Total young produced '5 '-l I 4 S 3 "l \ ~ ~.Final Adult Mortalitv I.. '- \. '- \... \. '-- '-- '- '--

~ ,J

qI

i.e]

,• J

· ,.J

'~ j

· ,

,,;~ J

Note. Adult mortality (L - live, D dead), sa spilt brood (smgle brood split between two days). CO - carry over (offspnngcarried over with adult during transfer).

lodeFl<odontRo,"ew~V

K,lIovE.Ko,n,n

fA.1S;

Concentration:% Mortalitv: C7Mean Offspring/Female: !J.<.t% Reduction from Control: &'l.~7_

SOP ATl4 - Exhibil ATl4.1, revision 06-01-11

of 110

~."'.'" T·······S.··.....·E . ''77·:e '. ~ :.i: ..@) ~'f;;:;;"

o lutlons Inc..' .,. ntal Testing So ,:..J Envlronme

Verification of Ceriodaphnia Reproduction Totals

1000 mg NaCIIL~

DayRe (icate number

TotalI 2 3 4 5 6 7 8 9 10

I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 4 5 4 4 4 4 4 5 5 435 12 10 11 13 11 10 12 12 10 10 1116 0 0 0 0 0 0 0 0 0 0 07 15 16 14 15 16 13 17 15 13 15 149

Total 31 30 30 32 31 27 33 31 28 30 303

DayReplicate Dumber Total

I 2 3 4 5 6 7 8 9 10

I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 5 4 4 5 6 5 5 4 4 4 465 12 12 10 11 10 11 10 11 11 12 1106 0 0 0 0 0 0 0 0 0 0 07 17 17 15 13 15 18 15 18 16 17 161

Total 34 33 29 29 31 34 30 33 31 33 317

Control

1200 mg NaCIIL

DayReplicate Dumber

TotalI 2 3 4 5 6 7 8 9 10

I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 0 I I 2 I 3 3 I I 2 155 3 5 2 3 3 0 0 2 4 I 236 0 0 0 0 0 5 2 0 0 0 77 7 6 4 6 5 5 7 4 3 5 52

Total 10 12 7 11 9 13 12 7 8 8 97

600 mg NaCIIL~

DayReplicate number

TotalI 2 3 4 5 6 7 8 9 10

I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 4 4 5 4 4 5 4 4 6 4 44

5 12 12 10 10 10 12 11 10 12 11 1106 0 0 0 0 0 0 0 0 0 0 07 17 16 15 15 18 17 18 16 16 15 163

Total 33 32 30 29 32 34 33 30 34 30 317

NaCIIL1400~ ._u m~

DayRe licate Dumber

TotalI 2 3 4 5 6 7 8 9 10

I 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 0 0 I 0 I 0 I I 0 0 45 0 1 0 1 0 2 0 0 3 2 96 0 0 0 0 0 0 0 0 0 0 07 5 3 0 3 4 I 3 0 0 2 21

Total 5 4 I 4 5 3 4 I 3 4 34

800 mg NaCIIL~

DayRe ticate number

TotalI 2 3 4 5 6 7 8 9 10

1 0 0 0 0 0 0 0 0 0 0 02 0 0 0 0 0 0 0 0 0 0 03 0 0 0 0 0 0 0 0 0 0 04 5 5 4 5 5 4 4 5 4 4 455 10 11 10 13 11 12 10 10 12 10 1096 0 0 0 0 0 0 0 0 0 0 07 18 16 14 14 16 15 13 18 16 17 157

Total 33 32 28 32 32 31 27 33 32 31 311

File: CdNaCICR_050812.xlsxEntered by: J. SumnerReviewed by: -Jf--

of 110

(8!D,.~H.I\~rjl

•••EYo~

T'I'" ':>,.:':.'j;j

","., ~

Environmental Testing Solutions, Inc.

Ceriodaphnia dubia Chronic Reference Toxicant TestEPA-821-R-02-013, Method 1002.0

Qnality ControlVerification of Data Entry, Calcnlations, and Statistical Analyses

Test Dumber: CdNaCICR #134

Test dates: May 08-15, 2012

Concentration Renlicate number Survival Average reproduction Coefficient of Percent reduction from

(mglL NaCl) I 2 3 4 8(%) (offspring/female) variation (%) control ("k)

5 6 7 9 10

Control 34 33 29 29 31 34 30 33 31 33 100 31.7 6.1 Not applicable

600 33 32 30 29 32 34 33 30 34 30 100 31.7 5.8 0.0

800 33 32 28 32 32 31 27 33 32 31 100 31.1 6.5 1.9

1000 31 30 30 32 31 27 33 31 28 30 100 30.3 5.8 4.4

1200 10 12 7 II 9 13 12 7 8 8 100 9.7 22.8 69.4

1400 5 4 1 , 4 5 3 4 1 3 4 100 3.4 42.1 89.3

Dunnett's MSD value:PMSD:

1.9276.1

MSD~

PMSD~

Minimum Significant DifferencePercent Minimum Significant DifferencePMSD is a measure of test precision. The PMSD is the minimum percent difference between the control and treatment that can be declared statisticallysignificant in a whole effluent toxicity test.

Lower PMSD bound detennined by USEPA (lalb percentile) = 13%.

Upper PMSD bound detennined by USEPA (90th percentile) = 47%.Lower and upper PMSD bounds were detennined from the 10th and 90th percentile, respectively, ofPMSD data from EPA's WET InterlaboratoryVariability Study (USEPA, 2001a; USEPA, 200Ib),

USEPA, 20010, 2001b, Final Report: Interlaboratory Variability Study ofEPA Short-term Chronic and Acute Whole Effluent Toxicity Test Methods, Volumes 1 and 2-Appendix, EPA-821-B-OI-004 and EPA-821-B-OI-005,

US Environmental Protection Agency, Cincinnati, OR.

File: CdNaCICR_050812,xlsxTable populated from associated "Verification of Ceriodaphnia Reproduction Totals" spreadsheet.

Spreadsheet entered by: J. SumnerReviewed by: --Jf--

of 110

eElS•..3< . "( .. ''"''...:-:. Environmental Testing Soluth>ns, Inc.


Cerlodaphnia Survival and Reproduction Test-ReproductionStart Date: 5/8/2012 Test 10: CdNaCICR Sample 10: REF-Ref ToxicantEnd Date: 5/15/2012 lab 10: ETS-Envir. Testing Sol. Sample Type: NACl-Sodium chlorideSample Date: Protocol: FWCHR-EPA-821~R-02-013 Test Species: CD-Ceriodaphnia dubiaComments:Conc-mQlL 1 2 3 4 5 6 7 8 9 1.

D-Control 34.000 33.000 29.000 29.000 31.000 34.000 30.000 33.000 31.000 33.000600 33.000 32.000 30.000 29.000 32.000 34.000 33.000 30.000 34.000 30.000800 33.000 32.000 28.000 32.000 32.000 31.000 27.000 33.000 32.000 31.000

1000 31.000 30.000 30.000 32.000 31.000 27.000 33.000 31.000 28.000 30.0001200 10.000 12.000 7.000 11.000 9.000 13.000 12.000 7.000 8.000 8.0001400 5.000 4.000 1.000 4.000 5.000 3.000 4.000 1.000 3.000 4.000

Transform: Untransformed 1-Tailed IsotonicCone-mall Mean N-Mean Mean Min Max CV% N t-Stat Critical MSo Mean N-Mean

D-Control 31.700 1.0000 31.700 29.000 34.000 6.140 10 31.700 1.0000600 31.700 1.0000 31.700 29.000 34.000 5.769 10 0.000 2.287 1.927 31.700 1.0000800 31.100 0.9811 31.100 27.000 33.000 6.511 10 0.712 2.287 1.927 31.100 0.9811

1000 30.300 0.9558 30.300 27.000 33.000 5.832 10 1.661 2.287 1.927 30.300 0.9558"'1200 9.700 0.3060 9.700 7.000 13.000 22.821 10 26.109 2.287 1.927 9.700 0.3060"'1400 3.400 0.1073 3.400 1.000 5.000 42.054 10 33.586 2.287 1.927 3.400 0.1073

Auxiliary Tests Statistic Critical Skew KurtKolmogorov D Test indicates normal distribution (p > 0.01) 1.02293 1.035 -0.3894 -0.8256Bartlett's Test indicates equal variances (p = 0.88) 1.79808 15.0863Hypothesis Test (1-tail. 0.05) NoEC LOEC ChV TU MSDu MSDp MSS MSE F-Prob dfDunnett's Test 1000 1200 1095.45 1.92678 0.06078 1662.66 3.55 3.7E-43 5,54Treatments vs D-Control

1500500 1000

Dosemg/L

1.0.,------------,

0.7

0.8

0.9

=0.6c8. 0.5

~ 0.4

0.3

0.2

0.1

0.0 ...,~_~....F't::;::;....~~...j

o

Linear Interpolation (200 Resamples)95%CL Skew

703.535 1009.92 -1.52511002.53 1024.94 -11.38481018.68 1040.35 -0.22991034.69 1055.49 -0.16851050.98 1071.19 -0.10221097.81 1118.78 0.07631129.3 1150.27 0.1443

91.461116.91625.718675.475135.296935.209535.52815

somg/L1001.8

1017.181032.571047.961063.351109.511140.29

PointIC05IC10IC15IC20IC25IC40IC50

Dose-Response Plot

Ind'p."dontlloYfewbV

"~'"

40

35

30

~ 25,e20..&! 15

10

5

0

£i 0 0 0 0 00 0 0 0 0

c '" '" := N ..8 r rC

1-tail,0.05Ievelof significance

File: CdNaCICR_050812.xlsxEntered by: 1.s~

ReviewedbY:~.....

of 110

I'1.I

IPage 5 of6

Species: Ceriodaphnia dubia CdNaCICR #: \~'-I

•

SOP ATl4 - Exhibit ATl4.1, revision 06-01-11

Initial

Jo6000STOCK

1200 mg NaCIIL

1400 mg NaCIIL

Concentration

cJ',,-1

J CONTROL (,,:!>,c... J

8&, 1,u

qU 600 mg NaCIIL

r,- J

L j

~ -~

"- ; 800 mg NaCIIL

,

1c J

1000 mg NaCIIL

of 110

0-1,, j UETS

~ Environm,nlOlTosUng S"uri."~ Inc.

Page 6 of6

Species: Ceriodaphnia dubia CdNaCICR #: I ~'-l

FinalInitialFinal

1.~. 'iInitial

2.~/.1

Final

1S.0InitialFinalInitial

CONTROL

600 mg NaCIIL

800 mg NaCIIL

1000 mg NaCIIL

1200 mg NaCIIL

1400 mg NaCIIL

-1L 1 Concentration

~] r--------,-------=--------,

1'- j

]~ ]

n:] I--__--+~~-+_=l.\.~\.g:__l~~~~~~~~......._:~~~~:_+_~~~~~

,.qy

. II

, J

. I ,t.j. g,J 1------.....~:-:-:-:--+~l~.q~S-+'?!"':'l"':--lh~~+F:""=--f.....,.I""r.~-If-,;;-:~-+"""':'~~~f'7'1'"':::~-1

lS

I"doper>dentlb>vI,wbV

~"I.VE.lC&.n.n

I.-tt SOP ATl4 - Exhibit ATl4.\, revision 06·0\·\\


DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - Mav 2012 DMR



DATE: 6/8/2012

CONCURRENCES

Name R

V

C

N


B.M. Love X U(~—^—Vl^p^ -^ (i/frl'Z.L.M. Koby X to /"[il^-J.W. Sabados X Q*-£i~ ^ s*db*A±^ c>l&llx-

INSTRUCTIONS: Originatorwill determine the review/concurrence assignment.

REVIEW: Examine technical content and commitments made. A

review (RV) should confirm the truth and accuracy of factual statements andindicate agreement with commitments made which are applicable to thereviewer's organization.



July 12, 2012


and Conservation




Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) -NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FORJUNE 2012

Enclosed is the June 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant.There were no exceedances during the monitoring period.

In the Sequoyah Nuclear Plant Unit 2 Cycle 18 refueling outage of October 2012, TVA willreplace the unit's four steam generators. During this large maintenance project, each of thesteam generators will be cut free from existing piping, lifted out from the top of the concreteshield building, and maneuvered through the steel containment and internal structuralconcrete enclosures that house the steam generators. Cutting the temporary openings inthe shield building and structural concrete enclosures using hydro-demolition will result inthe generation of concrete rubble and hydro-demolition process waste water for disposal.Up to 1.5 million gallons of water supplied by SQN's existing Fire Protection System(Hixson Utility District potable water) will be used for hydro-demolition over a 7 day period.The hydro-demolition waste water will be filtered to 5 micron and the pH will be adjustedpriorto flowing through SQN's normal waste water treatment process. As previouslydiscussed with Bob Alexander with the Division, TVA does not believe the activityassociated with replacing the steam generators will significantly change the nature orincrease the quantity of pollutants discharged from permitted outfalls. SQN will continue tomonitor Internal Monitoring Point 103 in accordance with NPDES permit TN0026450.


Ms. Christina MorganPage 2July 12,2012

/ certify under penalty of law that this document and all attachments were prepared undermy direction or supervision in accordance with a system designed to assure that qualifiedpersonnel properly gather and evaluate the information submitted. Based on my inquiry ofthe person or persons who manage the system, or those persons directly responsible forgathering the information, the information submitted is, to the best of my knowledge andbelief, true, accurate, and complete. I am aware that there are significant penalties forsubmitting false information, including the possibility of fine and imprisonment for knowingviolations.

John iinjarnn

Site Vice PresidentSequoyah Nuclear Plant

Enclosures




S.D. Booker, MOB 1F-WBNB. E. Brickhouse, BR 4A-CJ. T. Carlin, OPS 4A-SQNJ. A. Cross, POB 2A-SQNT.R. Markum, BR 4A-CD. B. Nida, BR 4A-C



J.W. Proffitt, OPS 4C-SQNA. A. Ray, WT11A-KG. R. Signer, WT 6A-KP.R. Simmons, POB 2B-SQNB. N. Smith (EDMS), MPB 1E-M


July 12, 2012


and Conservation




Dear Ms. Morgan:

TENNESSEE VALLEY AUTHORITY (TVA) - SEQUOYAH NUCLEAR PLANT (SQN) -NPDES PERMIT NO. TN0026450 - DISCHARGE MONITORING REPORT (DMR) FORJUNE 2012

Enclosed is the June 2012 Discharge Monitoring Report for Sequoyah Nuclear Plant.There were no exceedances during the monitoring period.

In the Sequoyah Nuclear Plant Unit 2 Cycle 18 refueling outage of October 2012, TVA willreplace the unit's four steam generators. During this large maintenance project, each of thesteam generators will be cut free from existing piping, lifted out from the top of the concreteshield building, and maneuvered through the steel containment and internal structuralconcrete enclosures that house the steam generators. Cutting the temporary openings inthe shield building and structural concrete enclosures using hydro-demolition will result inthe generation of concrete rubble and hydro-demolition process waste water for disposal.Up to 1.5 million gallons of water supplied by SQN's existing Fire Protection System(Hixson Utility District potable water) will be used for hydro-demolition over a 7 day period.The hydro-demolition waste water will be filtered to 5 micron and the pH will be adjustedprior to flowing through SQN's normal waste water treatment process. As previouslydiscussed with Bob Alexander with the Division, TVA does not believe the activityassociated with replacing the steam generators will significantly change the nature orincrease the quantity of pollutants discharged from permitted outfalls. SQN will continue tomonitor Internal Monitoring Point 103 in accordance with NPDES permit TN0026450.

If you have any questions or need additional information, please contact Brad Love byemail at [email protected] or by phone at (423) 843-6714.

Ms. Christina MorganPage 2July 12, 2012

/ certify under penalty of law that this document and all attachments were prepared undermy direction or supervision in accordance with a system designed to assure that qualifiedpersonnel properly gather and evaluate the information submitted. Based on my inquiry ofthe person or persons who manage the system, or those persons directlyresponsible forgathering the information, the information submittedis, to the best of my knowledge andbelief, true, accurate, and complete. I am aware that there are significant penalties forsubmitting false information, including the possibility of fine and imprisonment forknowingviolations.

^PresidentSequoyah Nuclear Plant

Enclosures

cc (Enclosures):Chattanooga Environmental Field Office U.S. Nuclear Regulatory CommissionDivision of Water Pollution Control Attn: Document Control DeskState Office Building, Suite 550 Washington, DC 20555540 McCallie Avenue



TN0026450 101 G


From

MAJOR

(SUBR01)

F - FINAL

DIFFUSER DISCHARGE

EFFLUENT

*** NO DISCHARGE [

Form Approved.

OMB No. 2040-0004

PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different)Name _ JVA ^EQ^^HJ^CLE^R^I^NTjMdress__P.a_BOX2p00.

aNJOEROFRCEOPSlN^QN)SODPX-DAISYJTN_37384

Facj!jty_ JA^-^QUOY^NUCLEAR PLANJLo<atiQEL Ji^lUPiLQQUNJY

ATTN: Brad Love

MONITORING PERIOD

YEAR MO I DAY

To

YEAR MO DAY

12 06 I 01 12 06 30NOTE: Read instructions before completing this form.

PARAMETER


00010 1 0

EFFLUENT GROSS


00010 Z 0

INSTREAM MONITORING


UPSTRM DEG.C

00016 1 S

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS


50060 1 0

EFFLUENT GROSS


82234 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

QUANTITY OR LOADING

AVERAGE

wwwwwwww

ftftftftftftftft

ftftftftftftftft

MAXIMUM

1741

Req. Mon.DAILY MAX

2

DAILY MX

UNITS

03

MGD

62

DEG

C/HR

MINIMUM

#♦♦♦♦♦♦#

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penaltyof lawthat this documentand ailattachmentswere preparedunder mydirection or supervisioninaccordancewitha systemdesigned to assure that qualified personnelproperlygather and evaluate the information submitted.Based on myinquiry of the person orpersons who manage the system, or those persons directlyresponsible for gathering theinformation, the information submitted is, to the best of myknowledgeand belief, true, accurate,and complete. I am aware that there are significantpenalties for submittingfalse information,includingthe possibilityof fine and imprisonmentfor knowingviolations.

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference allattachments here)No closed mode operation. Veliger monitoring data is included as an attachment. The following injections occurred: 1.Floguard MS6236 (max. calc. cone, was 0.059mg/L-limit 0.2mg/L) 2. Biodetergent73551 (max. calc.cone, was 0.033mg/L-limit 2.0mg/L) 3. SpectrusCT1300 (max. calc. cone,was 0.037mg/L-limit 0.050mg/L)EPA Form 3320-1 (REV 3/99) Previous editions may be used


AVERAGE

4t ft ft ft ft ft ft ft

ftftftftftftftw

0.024

0.1

MOAVG

MAXIMUM

41.0

Req. Mori.DAILY MAX

30.4

30.5

DAILY MX

3

DAILY MX

0.051

0.1

DAILY MAX

UNITS

04

DEG.C.

04

DEG.C.

04

DEG.C.

19

MG/L

NO.

EX

FREQUENCYOF

ANALYSIS

30/30

CONTI

NUOUS

30/30

CONTI

NUOUS

30/30

CONTI

NUOUS

30/30

CONTI

NUOUS

26/30

FIVE PER

WEEK

30/30

CONTI

NUOUS

SAMPLE

TYPE

RCORDR

CALCTD

MODELD

CALCTD

CALCTD

CALCTD

RCORDR

RCORDR

GRAB

CALCTD

CALCTD

CALCTD

TELEPHONE DATE

Vice President


423 843-7001

AREA

CODENUMBER

12 07

YEAR MO

Page 1 of 1

11

DAY

Sample DateMean# of

ZM/m3% Settlers

Water

Temp. ('C)Sample Date

Mean# of

Asiatic

Clams/m3

Water

Temp. (°C)

NOTES: %

LOCATION SUB LOCATION Gravid Asiatic

Clam

COLLECTED BY

01/03/2012 14 100 26 01/03/2012 0 26 1-25-545 PKS

01/10/2012 0 0 9 01/10/2011 0 9 RCW WBE

01/17/2011 0 0 10 01/17/2011 0 10 1-ISV-24-1234 PB

01/24/2012 0 0 13 01/24/2012 0 13 1-25-545 WDT

01/31/2012 0 0 17.6 01/31/2012 0 17.6 1-25-545 CR

02/07/2012 0 0 12 02/07/2012 0 12 1-25-545 BB

02/14/2012 0 0 8.3 02/14/2012 0 8.3 1-24-1234 WE

02/21/2012 0 0 26.5 02/21/2012 0 26.5 1-25-545 CR

02/28/2012 0 0 11.1 02/28/2011 0 11.1 1-ISV-24-1234 WBE

03/06/2012 0 0 11.7 03/06/2012 0 11.7 1-ISV-24-1234 WBE

03/13/2012 0 0 13 03/13/2012 0 13 1-ISV-24-1234 WBE

03/20/2012 0 0 14.6 03/20/2012 0 14.6 1-ISV-24-1234 WBE

03/27/2012 1623 1.3 17.2 03/27/2012 0 17.2 1-ISV-24-1234 WBE

04/03/2012 229 0 18 04/03/2012 0 18 1-ISV-24-1234 PB

04/10/2012 79 20 22 04/10/2012 0 22 1-ISV-24-1234 PB

04/18/2012 326 5 18.8 04/18/2012 0 18.8 1-ISV-24-1234 MJW

May 2012 No Samples CollectedJune 2012 No Samples Collected

PERMITTEE NAME/ADDRESS (Include Facility Name/Location if Different)Name _ JVA-Srai^WH_rW(^^FÎÔT^djBSS_-P.a_BOX2000.

aitreEQFRCEOPS^r^sQN)SODr^-JÎSYJlN_37384

Faa|jty__ JVA.- SEQUOYi^NUCLEAR PLANJJ=2fiatLoji_JjJWi^

ATTN: Brad Love


From

TN0026450 101 T


/IONITORING PERIODYEAR MO DAY

To

YEAR MO DAY

12 06 01 12 06 30

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE

Form Approved.

OMB No. 2040-0004

NOTE: Read instructions before completing this form.PARAMETER


EX

FREQUENCYOF

ANALYSIS

SAMPLE



CERIODAPHNIA

TRP3B 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

♦♦♦♦*♦♦♦ "ttHftfttltlHtlt** Monitoring

Not Required******** ******** 23


**♦♦•♦••• w&ftftftftfttt / 43.2

MINIMUM

£4fc4nfc&4?ft ,$tftftftwftft4t, SEMI

ANNUAL

COMPOS


PIMEPHALES

TRP6C 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

~™ w4rwwwwww** Monitoring

Not Required

™~ _. . ......

23

PERCENTPERMIT

REQUIREMENT

iHkfc^HHHMfe wtWtWKMMWW 43.2

MIMINUM

4HkMHkA&& Aftft&ft ft ftft. SEMI

ANNUAL

COMPOS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

NAMEmTLE PRINCIPAL EXECUTIVE OFFICER

John T. Carlin


TYPED OR PRINTED

I Certify underpenaltyof lawthat this documentand all attachmentswere prepared undermydirectionor supervision in accordance witha system designed to assure that qualifiedpersonnelpropertygather and evaluate the information submitted.Based on myinquiryof the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. I am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowingviolations.

COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here)Toxicity was not sampled in June 2012.


SIGNATORE'bF PRINCIPAL EXECUTIVEOFFICER OR AUTHORIZED AGENT

TELEPHONE

423 843-7001

AREA

CODENUMBER

DATE

12 07

YEAR MO

Page 1 of 1

11

DAY


TN0026450 103 G


From

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE [



Name __™AÊQI^^HJ^C^^RPLANT^di§s^^.O^BOX2000_

aNJEROmCEOPSÛSQN)SODDYrDAISY1XN_37384

facmy_ JVA.r^QyOY^NUCLEAR PLANJLocMoJl-Jd>ÎIJPiLQOUJ£rY

ATTN: Brad Love


To

YEAR MO DAY

12 06 01 12 06 30NOTE: Read instructions before completing this form.

PARAMETER

PH

00400 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

OIL AND GREASE

00556 1 0

EFFLUENT GROSS


50050 1 0

EFFLUENT GROSS

SAMPLEMEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

QUANTITY OR LOADING

AVERAGE

♦♦♦♦♦♦♦♦

0.966

Req. Mon.MOAVG

MAXIMUM

1.231

Req. MonDAILY MX

UNITS

03

MGD

MINIMUM

6

MINIMUM

AftJfcftftft4fA>

********

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penalty of law that this document and all attachments were prepared under mydirection or supervision in accordance with a system designed to assure that qualified personnelproperly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. I am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.


AVERAGE

30

MOAVG<6

15

MOAVG

MAXIMUM

MAXIMUM

100

DAILY MX

<6

20

DAILY MX

Vice President

UNITS

12

SU

19

MG/L

19

MG/L

NO.

EX

TELEPHONE

FREQUENCYOF

ANALYSIS

14/30

THREE/

WEEK

2/30

TWICE/

MONTH

2/30

TWICE/

MONTH

30/30

SEE

PERMIT

SAMPLE

TYPE

GRAB

GRAB

GRAB

GRAB

GRAB

GRAB

RCORDR

RCORDR

DATE

423 843-7001 12 07 11

John T. Caiiin


TYPED OR PRINTED

SIGNATURE OF PRINCIPAL EXECUTIVE

OFFICER OR AUTHORIZED AGENT AREA


COMMENTS AND EXPLANATION OFANY VIOLATIONS (Reference allattachments here)



Name ___™A-SEQW^HJ^C^^RPLANT^dr^^JPO^BOXJOOO.

aNJEROFRCEOPSlN^QN)SODDY.- DAJSYJK37384

FacHjty_ J>^-^QUOY>^NUCLEAR PLANJU(^on_JiAMiypiLQQUi£rY

ATTN: Brad Love


MAJOR

(SUBR01)

F - FINAL


EFFLUENT

*** NO DISCHARGE


TN0026450

PERMIT NUMBER

From

110 G

DISCHARGE NUMBER

XX|

MONITORING PERIOD

YEAR MO DAY

To

YEAR MO DAY

12 06 01 12 06 30NOTE: Read instructions before completing this form.


EX

FREQUENCYOF

ANALYSIS

SAMPLE



00010 1 0



00010 Z 0

INSTREAM MONITORING


UPSTRM DEG.C

00016 1 0



50050 1 0



50060 1 0



82234 1 0


SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

♦♦♦♦♦♦♦♦

vMnMnMntw

Req. Mon.DAILY MX

2

DAILY MX

03

MGD

04

DEGC

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER ICertify under penaltyof lawthat this documentand all attachments were prepared under mydirectionor supervision in accordance witha system designed to assure that qualifiedpersonnelproperlygather and evaluate the information submitted. Based on my inquiryof the person orpersons who manage the system, or those persons directlyresponsible forgathering theinformation, the information submitted is, to the best of myknowledgeand belief, true, accurate,and complete. Iam aware that there are significantpenalties for submittingfalse information,including the possibility of fine and imprisonment for knowing violations.

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OFANYVIOLATIONS (Reference allattachments here)No Discharge this Period


mWWttwtltWit

0.1

MOAVG

REPORTDAILY MX

30.5

DAILY MX

5

DAILY MX

0.1

DAILY MX

04

DEGC

04

DEGC

04

DEGC

19

MG/L

TELEPHONE

Vice President423 843-7001


CODENUMBER

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

CONTIN

UOUS

Five per

Week

CONTIN

UOUS

CALCTD

CALCTD

CALCTD

RCORDR

CALCTD

CALCTD

DATE

12 07 11

YEAR MO DAY

Page 1 of 1


Name _ JVA ^^^^^N^CL^RJ^LANTAddress_ .P.O^BOXgpOO.

aÊQEOQEQE^riSQN)SOD^-DAjSYJlN^^

Fac||ity__T>^-SEf^^LgratLon_ Ji>ÎUPJiCOUNTY

ATTN: Brad Love


From

TN0026450 110 T



To

YEAR MO DAY

12 06 01 12 06 30

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE XX




EX

FREQUENCY

OF

ANALYSIS

SAMPLE

TYPE


CERIODAPHNIA

TRP3B 1 0 0



PIMEPHALES

TRP6C 1 0 0


SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT


'KlUtftWwtwtWt

MINIMUM

43.2

MINIMUM

43*2

MINIMUM


23

PERCENT

23

PERCENT

SEMI

ANNUAL

SEMI

ANNUAL

COMPOS

COMPOS

NAME/TITLE PRINCIPAL EXECUTIVE OFFICER 1Certifyunder penalty of lawthat this document and all attachments were prepared under mydirection or supervision in accordance with a system designed to assure that qualified personnelproperly gather and evaluate the information submitted. Based on my inquiryof the person orpersons who manage the system, or those persons directly responsible for gathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. 1am aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.

[StoSoy^sr&Vt8e President

TELEPHONE DATE

John T. Carlin

Sequoyah Site Vice President 423 843-7001 12 07 11SIGlfatURÔF PRINCIPAL EXECUTIVE

OFFICER OR AUTHORIZED AGENTTYPED OR PRINTED

AREA





PERMITTEE NAME/ADDRESS (Include Facility NameAocation if Different)Name _ JTVAJfEQ^^HJ^CLF^RJ^NTjMdr^s__P.a_BOX2000.

(LNIPROFFiQE OPS£NJ>QN)SQDr^-JMSYJ^7^

FacHjty_ _TVA- SEQUOY^NUCLEAR PLANJJLsi^on_Ji>^IUPJLQQyi£TY

ATTN: Brad Love


From

TN0026450

PERMIT NUMBER

118 G

DISCHARGE NUMBER


ToYEAR MO DAY

12 06 01 12 06 30

MAJOR

(SUBR01)

F - FINAL


EFFLUENT

NO DISCHARGE XX

Form Approved.

OMB No. 2040-0004



EX

FREQUENCYOF

ANALYSIS

SAMPLE

TYPE


00300 1 0

EFFLUENT GROSS


00530 1 0

EFFLUENT GROSS

SOLIDS, SETTLEABLE

00545 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

PERMITREQUIREMENT

SAMPLE

MEASUREMENT

AVERAGE MAXIMUM UNITS MINIMUM

2

MINIMUM

Aftftftftftftft

AVERAGE

"kitMHkft ft)Nt

IHtWKwtltlUt

MAXIMUM

100

DAILY MX

UNITS

19

MG/L

19

MG/L

25

MUL

TWICE/

WEEK

TWICE/

WEEK

GRAB

GRAB

GRABPERMITREQUIREMENT

DAILY MX

ONCE/

MONTH


50050 1 0

EFFLUENT GROSS

SAMPLE

MEASUREMENT 03

MGD

******** WWWKWRXW

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

SAMPLE

MEASUREMENT

PERMIT

REQUIREMENT

Req. Mon.MOAVG

Req. Mon.DAILY MX

NAMEHTTLE PRINCIPAL EXECUTIVE OFFICER Certify under penalty of law that this document and all attachments were prepared under mydirectionor supervision in accordance with a system designed to assure that qualifiedpersonnelproperly gather and evaluate the information submitted. Based on my inquiry of the person orpersons who manage the system, or those persons directlyresponsible for gathering theinformation, the information submitted is, to the best of my knowledge and belief, true, accurate,and complete. Iam aware that there are significant penalties for submitting false information,including the possibility of fine and imprisonment for knowing violations.

John T. Carlin


TYPED OR PRINTED

COMMENTS AND EXPLANATION OF ANYVIOLATIONS (Reference all attachments here)During this reporting period, there has been no flow from the DredgePond other than that resulting from rainfall.


wtWWWWWmW

SIGNATURE OF PRINCIPAL EXECUTIVE

OFFICER OR AUTHORIZED AGENT

TELEPHONE

423 843-7001

AREA

CODENUMBER

ONCE/

BATCH

ESTIMA

DATE

12 07 11

YEAR MO DAY

Page 1 of 1


DOCUMENT NAME: SEQUOYAH NUCLEAR PLANT - June 2012 DMR



DATE: 7/10/2012

CONCURRENCES

Name R

V

C

N


B.M. Love X %Gf^ ^p?^ 7/«•/'£L.M. Koby X 7/«fi^J.A. Cross X >f/jAJU*U{/i(P<> ' '' 1


REVIEW: Examine technical content and commitments made. A

review (RV) should confirm the truth and accuracy of factual statements andindicate agreement with commitments made which are applicable to thereviewer's organization.


SQN Annual Water Withdrawal Updates for January 2012.

Documents

Transcript of SQN Annual Water Withdrawal Updates for January 2012.