MIDWEST SOCIETY OF PERIODONTOLOGY

2016-2017 OFFICERS & EXECUTIVE

COMMITTEE

PRESIDENTDr. Paula Weistroffer

Iowa City, IA

PRESIDENT ELECTDr. Tae-Ju OhAnn Arbor, MI

VICE PRESIDENTDr. Darnell Kaigler

Detroit, MI

SECRETARY Dr. Nabil Bissada

Cleveland, OH

TREASURERDr. Paul Luepke

Highland Park, IL

PAST PRESIDENTDr. Diego Velasquez

Fenton, MI

COUNCILPERSONSDr. Gustavo Avila-Ortiz

Iowa City, IA

Dr. Ebone JordanMilwaukee, WI

Dr. Megan Ratliff Glenview, IL

CONTENTSPresident’s Message ...........1AAP Elections ........................2Meeting Sponsors ...............2Meeting Photos ....................3Research Forum Abstracts .............................4-6Graduate Student

Spring 2016 | Volume 60 | No. 1 msperio.org

M I D W E S T S O C I E T Y O F P E R I O D O N T O L O G Y N E W S L E T T E R

PRESIDENT’S MESSAGE

Greetings from Iowa City!

Spring is just arriving at the University of Iowa and every-thing is beginning to turn beautiful shades of green. In an effort to be more green ourselves, you will notice that we are sending this newsletter in electronic format only. This not only helps the environment, but it saves valuable resources on printing. We will do this again for a total of 2 of our 3 newsletters in electronic for-mat this year. Also, I would like to draw your attention to the new website. It has been redesigned, improved and will continue to be updated over time. It should become a great resource for current and prospective members. If you haven’t seen it yet, check it out at www.msperio.org.

We had a wonderful 59th annual meeting at the end of February. We were back home, so to speak, at the newly renovated Renaissance Hotel. Next year should bring even more changes to our venue as their renovation continues. Thanks to our immediate past President Dr. Diego Velasquez and his Pro-gram director, and my esteemed colleague, Dr. Gustavo Ávila Ortiz, for their organization and planning of this year’s meeting. It will be a tough one to top! We also heard about the exciting research occur-ring in our periodontal programs across the district, in two different forums this year. Dr. Michael Fran-zman organized this year’s Graduate Student Research Forum. This year’s top 3 abstracts for our oral competition were from Dr. Sukirth Ganesan from The Ohio State University, Dr. Alberto Monje from the University of Michigan and Dr. Samia Suleiman-Ata from the University of Illinois. But this year, we also added a new poster competition. We had many excellent submissions and the top 5 abstracts selected for posters were from Dr. Ann Dekker, Dr. Carlos Garaicoa Pazmino and Dr. Fernando Suarez, all from the University of Michigan, Dr. Monica Gibson from The Ohio State University and Dr. Krithika Rajkumar from Indiana University. We felt that this addition was a great opportunity to showcase more research projects and give residents another opportunity to present. I think it was a great success. Thanks again to our judges this year, Dr. Tricia Crosby, Dr. Jonathan Jenkins and Dr. Arndt Guentsch. Also, congratula-tions again to the two winners this year, Dr. Samia Suleiman-Ata for the oral competition and Dr. Ann Decker for the poster competition.

Be sure to save the date now on your calendars for next year’s meeting! The 60th annual MSP meeting will be held February 24-26th, 2017. We will be hearing from Dr. George Mandelaris and his group about Surgically Facilitated Orthodontic Therapy. Dr. Franzman and I are working on a hands-on program for Friday and a practice management component for Sunday. This will be a great meeting to invite your colleagues or even better, to come as a team.

Before I close, I want to take a moment to thank those that have come before me, but especially our immediate past President, Dr. Diego Velasquez and our Executive Director, Dr. Greg Fauth. These people are such role models for me. Diego’s graciousness and calm manner are what I hope to achieve as your president. Greg is doing such an amazing job as our Executive Director and keeps us all on track with the myriad of details that it takes to run this organization and produce the high quality meetings you have come to know and expect.

I have been involved in this great organization for 8 years now, soon after I began my academic ca-reer here at the University of Iowa. It is a marvelous group of people to work with and we all have your best interests at heart. Each spring is a like a new beginning. I look forward to this coming year and am very much honored to serve as your president.

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A A P E L E C T I O N SThe AAP Elections are during the month of June, 2016

District 4 ballots will have options for:

SECRETARY-TREAURER

Bryan Frantz, Pennsylvania Richard Kahn, New Jersey

ABP DIRECTOR (VOTE FOR TWO)

Robert M. Eber, Ann Arbor, MI James A. Katancik, Portland, OR

OFFICER NOMINATING COMMITTEE

Peter Cabrera, Chicago, IL

DISTRICT 4 TRUSTEE (VOTE FOR ONE)

Jeffrey Johnston, Sterling Heights, MI Edward Segal, Northbrook, IL

DISTRICT 4 TRUSTEE (VOTE FOR ONE)

Vanchit John, Indianapolis, IN Stephen Meraw, Warren, MI

A W A R D T O T H E P A S T P R E S I D E N TDr. Paula Weistroffer (L), Iowa City, IA, President Midwest Society of Periodontology presents Dr. Diego Velasquez (R), Fenton, MI, Immediate Past President with a plaque of appreciation.

G E N E R A L M E E T I N G S U P P O R TWe want to express our thanks to the exhibitors who support the Midwest Society of Periodontology by participating in our annual meeting. We appreciate their support of our Society.

SPONSORS

BioHorizons

Community Tissue Services

ConeScan

Dental USA, Inc.

Dentsply Implants, North America

Digital Dental Source, Inc.

DoWell Dental Products

Fotona

Galaxo Smith Kline Consumer Health Care

G. Hartzell & Son by DenMat

Geistlich Biomaterials

Hiossen, Inc.

Millennium Dental Technologies, Inc.

MIS Implant Technologies, Inc.

Nobel Biocare

Osada, Inc.

Osteogenics Biomedical

Piezosurgery, Inc.

Predictable Surgical Technologies

Productive Dentist Academy

Salvin Dental Specialties, Inc.

Snoasis Medical

Straumann USA

Sunstar Americas, Inc.

TekScan, Inc.

TePe Oral Health Care, Inc.

Thommen Medical, USA, L.L.C.

Treloar & Heisel, Inc.

Zimmer Biomet

THANK YOU!

R E S E R V E T H E D A T E !60th MSP Annual Meeting February 24-26, 2017RENAISSANCE HOTELChicago, IL

Spring 2016 | Volume 60 | No. 1 msperio.org | 3

Dr. Massimo Simion presented Saturday afternoon on “25 Years of Vertical Augmentation: Esthetic Considerations and New Developments”.

On Saturday morning, Dr. Anton Sculean presented, “Clinical Concepts in Regenerative and Plastic-Esthetic Periodontal Surgery”.

Dr. Greg Tobak presented Sunday morning on “HP3: Creating a High Performance Perio Practice”.

Dr. Chris Barwacz spoke at the Friday Limited Attendance Lecture on “Implant Provisionalization: Protocols for Maximizing Peri-Implant Mucosal Outcomes”.

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R E S E A R C H F O R U M A B S T R A C T SEFFECTS OF A PERIODONTAL PATHOGEN ON INSULIN SECRETION AND ISLET CELL APOPTOSIS

DR. SAMIA SULEIMAN-ATAUniversity of Illinois at Chicago, Chicago, IL

Background

Periodontitis is a chronic inflammatory condition that involves alveolar bone loss. Periodontitis is a host cell response to bacte-rial pathogens. Periodontal pathogens in time also disseminate from the oral cavity to other parts of the body, such as the liver, via systemic circulation. Epidemiologically, there is an associa-tion between Type 2 Diabetes and Periodontitis; patients that are comorbid with periodontitis and diabetes have marked severity in disease. However, the effect of periodontal patho-gens on the development of insulin resistance and prediabetes has yet to be clarified. Results from previous animal studies indicate that induction of chronic periodontitis in both rat and mouse models caused increased insulin secretion, hyperinsulinemia (HI), Insulin Resis-tance (IR) and Glucose Intolerance (GI), all of which are classic hallmarks of prediabetes. This demonstrates that periodontitis directly plays a role in the development of prediabetes.

Objective

The aim of this study is to determine the effect of Porphyromonas gingivalis (Pg) on insulin secretion, apoptosis and gene expression in pancreatic beta cells. We hypothesize Pg will cause an increase in insulin secretion and apoptosis in MIN6 beta cells and increase in gene expression associated with insulin secretion. Additionally, in vivo Pg-induced periodontitis in animals will cause hyperinsu-linemia and increase pancreatic islet cells apoptosis.

Methods

A pancreatic beta cell line MIN6 cells co-incubated with Pg for 0-24 hours were collected and assessed for insulin secretion, apoptosis, gene expression and protein expression using GSIS, ELISA, immunohistochemistry, PCR and western blot. Mice with Pg-induced periodontitis were evaluated at 23 weeks for fasting insulin levels and islet apoptosis. To determine the effect of Pg on the pancreas, we induced chronic periodontitis by oral ap-plication of Pg and showed development of hyperinsulinemia by the 14th week.

Results

Co-incubation of Pg with MIN6 cells induced upregulation of SNAP25 gene expression as well as insulin secretion. In addition, Pg induced apoptosis via both intrinsic and extrinsic pathways. Pg also increased TRB3 gene expression and downregulated AKT phosphorylation. Apoptosis of islet cells were detected at sacrifice (23 weeks) in mice with periodontitis. Apoptosis of islet cells were evident both in vitro and vivo.

Conclusion

This is the first report that a periodontal bacterial pathogen influences insulin secretion and also causes apoptosis of beta cells suggesting a potential mechanism by which periodontitis exacerbates the prediabetic condition.

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R E S E A R C H F O R U M A B S T R A C T SA TALE OF TWO RISKS: SMOKING, DIABETES, AND THE SUBGINGIVAL MICROBIOME

DR. SUKIRTH GANESAN The Ohio State University, Columbus, OH

Background

Smoking and diabetes are the only two established risk factors for periodontitis, and their effects on the subgingival biofilm have been independently studied. However the combined effect of these risk factors on the subgingival microbiome is unknown.

Objective

The aim of the current study is to analyze and compare the microbial composition of the subgingival biofilm in diabetics, smokers, and diabetic smokers with periodontal disease.

Methods

The study population consisted of 100 subjects with chronic generalized moderate to severe periodontitis, divided evenly between four groups classified based on glycemic index and smoking status: diabetics, smokers, diabetic smokers, and non-diabetic, non-smoking controls. Subgingival biofilm samples were collected from shallow (probe depths <3mm, attachment loss <1mm) and deep (probe depths >5mm, attachment loss >4mm) sites using endodontic paper points. Bacterial DNA was isolated and the V1-V3 and V7-V9 hypervariable regions of the 16S rRNA genes were sequenced using the 454 pyrosequenc-ing platform. 1.7million high-quality, classifiable sequences were compared to the Human Oral Microbiome Database using the QIIME and PhyloToAST pipelines. UniFrac distances were compared using community ordination methods. Additionally, networks were computed to examine bacterial co-occurrences. Statistical comparisons were made using Tukey HSD.

Results

Deep and shallow sites were phylogenetically similar in all four groups. Linear Discriminant Analysis (LDA) of Unifrac distances revealed distinct differences between the microbi-omes of smokers and diabetics; further, smokers were microbi-ally more homogenous than diabetics and diabetic-smokers. Keystone species that are selectively enriched for that habitat were analyzed. Species belonging to the genera Lactobacillus, Treponema, Fretibacterium and Bacteroidetes were significantly more abundant in smokers, while those belonging to Olsenella, Prevotella, Alloprevotella and Capnocytophaga demonstrated higher abundances in diabetics and diabetic-smokers. Diabetic-smokers exhibited the greatest microbial heterogeneity; and diverged significantly from both smokers and diabetics. Species belonging to Cardiobacterium, Enterobactor, Sphingomonas and Pseudomonas were significantly associated with diabetic-smokers. LDA also revealed distinct clustering of microbiomes in diabetics based on diabetic status (HbA1c levels), however smokers clustered irrespective of diabetic status.

Conclusion

Both smoking and diabetes are associated with a global shift in the disease-associated microbiome, not a site-specific shift. Smoking and diabetes each uniquely shape the oral microbi-ome. While smokers, diabetics and diabetic smokers all demon-strated pathogen-rich communities; the nature of enrichment was different. Microbial replacement occurs in response to smoking. Microbial succession occurs in response to hypergly-cemia. Combined effects of these risk factors are multiplicative.

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R E S E A R C H F O R U M A B S T R A C T S3D-PRINTED MICROPATTERNED SCAFFOLDS FOR PERIODONTAL TISSUE REPAIR

DR. ALBERTO MONJEUniversity of Michigan, Ann Arbor, MI

Background

The formation of tissues with structural and functional integrity is reliant upon cellular alignment and periodontal organization and is influenced by nano- and micro-scale environmental cues. Recently, 3D-printed resorbable scaffolds have been assessed for cellular alignment to promote aligned collagens fiber synthesis in vitro. However, there is lack of research regarding conditions neces-sary for patterning to promote in vivo aligned tissue formation for periodontal tissue repair.

Objectives

The purpose of this study was to develop a scaffold utilizing 3D-printing technology in combination with gene therapy to mimic the periodontal ligament (PDL)-alveolar bone complex in the oral cavity, which is susceptible to damage via chronic inflammatory to periodontitis, and to evaluate the ideal scaffold patterning for optimal PDL cell alignment.

Methods

Polymer fiber-guiding films were patterned using a custom-ized mold fabricated via soft lithography and scaffolds were 3D-printed using selective laser sintering. Scanning electron microscopy was used to visualize 3D patterns on poly-caprolac-tone acid (PCL) films. Films were hydrolyzed and fibronectin-treated prior to cell seeding and 3D printed polymer region was seeded with adenoviral BMP7-transduced fibroblasts prior

to subcutaneous implantation using a murine model. Bone volume and tissue mineral density (TMD) were analyzed using micro-CT at 3 and 6 weeks post-implantation. Aligned collagen thickness was assessed using H&E and Masson’s trichrome staining; tubulin and DAPI staining was performed for cellular elongation and nuclear orientation quantification.

Results

Our outcomes indicated statistically significant increases in bone TMD from weeks 3 to 6 (p<0.0001) with sustained bone volume at both time points. Percentage of aligned cells in vivo on 30um deep pillar grooves (35±8.3% to 39±6.8% for 60 and 15um wide grooves, respectively) was significantly higher compared to 10 um deep grooves (24±8.7% to 25±6.4%), in-cluding the control groups [non-grooved pillars (15±7.0%) and amorphous, non aligned scaffolds (9.1±3.6%)]. Pillars with 30um deep grooves had significantly (p<0.0001) higher oriented collagen thickness (29.1±10.0 to 33.7±15.1um) compared to non-grooved pillars (16.5±6.7um). These results indicate im-portance of patterning (groove depth over width) as a measure of cellular alignment and PDL collagen formation in vivo for tissue-engineered constructs utilizing 3D-printed scaffolds and gene therapy.

Conclusion

It can be concluded that this scaffold with 3D-printed and micropatterned regions in combination with gene therapy is capable of supporting bone formation and oriented collag-enous tissue formation in vivo for periodontal tissue repair. Such a design-oriented approach has potential for improving the re-generative response of bone-ligament complexes for interfacial tissue engineering.

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G R A D U A T E S T U D E N T R E S E A R C H F O R U M

At the February meeting of the MSP in Chicago the finalists of the Graduate Student Research Forum were all presented plaques and $500 checks at the awards ceremony and reception. The Research Forum and awards ceremony are supported by a gener-ous grant from Sunstar Americas, Inc.

FIRST PLACEDr. Samia Suleiman-Ata

HONORABLE MENTIONDr. Sukirth GanesanDr. Alberto Monje

POSTER COMPETITION FIRST PLACE

Dr. Ann DeckerUniversity of Michigan, Ann Arbor, MISympathetic Signaling Mediator, Norepi-nephrine, Re-Activates Prostate Cancer Bone Marrow Metastasis

POSTER COMPETITION HONORABLE MENTION

Dr. Monica Gibson - The Ohio State Univesity

Dr. Carlos Garaicoa-Pazmino - University of Michigan

Dr. Krithika Rajkumar - Indiana University

Dr. Fernando Suarez - University of Michigan

T H E M I D W E S T S O C I E T Y W I S H E S T O A C K N O W L E D G E S U P P O R TThank you to Event Sponsors for the 2016 meeting.SUNSTAR AMERICASGraduate Student Research Forum Awards Ceremony

DENTSPLY IMPLANTS, NORTH AMERICADr. Chris Barwacz, Friday’s Scientific Session Meeting Program

GEISTLICH AND OSTEOGENICS Dr. Anton Sculean, Saturday-Morning’s Scientific Session

STRAUMANNDr. Massmo Simion,Saturday Afternoon’sScientific Session

ZIMMER BIOMETDr. Greg Toback Sunday’sScientific Session

Mark Carlascio from Sunstar Americas stands next to Dr. Samia Suleiman-Ata, First Place Award Recipient. On the far right, is Dr. Sukirth Ganesan who stands next to Dr. Alberto Monje, both of whom were awarded Honorable Mentions.

S A V E T H E D A T E !60th MSP Annual Meeting, February 24-26, 2017RENAISSANCE HOTELChicago, IL

N E W M E M B E R STony Barirere, Columbus, OH Eric Brockman, Madison, WIBrandon Bushong, Belleville, ILAndrew Carmosino , Highland Park, ILAlbert Chan, Ann Arbor, MIBrad DeGroot , Oakbrook Terrace, ILSergey Dolgov, Mankato, MNRicardo Gonzalez , Rochester, MNYusuke Hamada , Indianapolis, INDawlat Hasso, Troy, MI

E. Dwayne Karateew , Chicago, ILShelia Kennebrew , Southfield, MIRebecca Kibler, Berwyn, ILHarrison Mackler, Chicago, ILShahir Malek , Saint Louis Park, MNVaughn McGraw, Petosky, MIAniruddh Narvekar, Chicago, ILJoseph Nemeth, Southfield, MITerrance Nessif, Toledo, OHErez Nosrati , Arlington Heights, IL

Monica Raina , Toronto, ON, CanadaKrithica Rajkumar, Carmel, INJuan C. Rodriguez , Ann Arbor, MIShawn Rotenberg , Columbus, OHEmily Schmalz, Rochester, MNKatherine Smith, Victoria, MNKelley Thompson , Prairie Village, KSWilliam Trahan, St. Charles, ILJeff Wang, Ann Arbor, MIYing Lien Wu, Maple Grove, MN