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Journal of Microencapsulation, 2012, 1–8, Early Online� 2012 Informa UK Ltd.ISSN 0265-2048 print/ISSN 1464-5246 onlineDOI: 10.3109/02652048.2012.680510
Spray drying of monodispersed microencapsulates: implications offormulation and process parameters on microstructural propertiesand controlled release functionality
Wenjie Liu1, Winston Duo Wu1, Cordelia Selomulya1 and Xiao Dong Chen1,2
1Department of Chemical Engineering, Monash University, VIC 3800, Australia and2Department of Chemical and Biochemical Engineering, College of Chemistry and ChemicalEngineering, Xiamen University, 361005 Fujian Province, P.R. China
AbstractParticulates for pharmaceutical applications require stringent control over their characteristics to realize theoptimal therapeutic performance. By generating uniform spray-dried silica particles encapsulating differentmodel drugs via a microfluidic jet spray drying technique, we demonstrated how the effects of formulationand process parameters on the investigated properties could be directly quantified without the complica-tions of wide particle distributions typical of conventional spray drying. The implemented strategiesincluded incorporating lactose to modify the internal microstructures to regulate release, and increasingdrying temperature during synthesis to modify the surface features of particles. The physicochemicalproperties of encapsulated drugs were shown to influence particle morphologies and release profiles,while the pH of initial precursors influenced the particle morphologies with slight effects on the initialrelease rates. The outcomes would be useful to indentify appropriate formulations and manufacturingparameters in designing spray-dried silica-based microencapsulates with tailor-made controlled releasefunctionalities.
Keywords: microfluidic jet spray drying, microencapsulation, controlled release, pharmaceutical particles,microstructure, design strategy
Introduction
Particulate-based dosage form is an important part of the
pharmaceutical and biotechnology industries (Vehring,
2008; Wang et al., 2009). These powders could be inhaled
as aerosols (Momeni and Mohammadi, 2009), pressed into
tablets (Corti et al., 2008), injected through a syringe needle
(Cevher et al., 2006) or delivered transdermally (El-Kamel
et al., 2007). For advanced therapeutic approaches, the par-
ticle properties are essential for the stabilization, transpor-
tation and activation of therapeutic ingredients
(Suksamran et al., 2009; Ye et al., 2010). Among the differ-
ent particle fabrication methods, spray drying offers the
advantages of rapid production and readily scalable pro-
cess (Sollohub and Cal, 2010), with almost no restriction for
the choice of excipient materials and drugs (Learoyd et al.,
2009), as long as the precursor solutions could be atomized.
For pharmaceutical applications with stringent require-
ments over the particle properties (e.g. size, morphology
and release modulation) (Tran et al., 2011), spray drying
does have some inherent limitations. Since conventional
atomizers generally generate droplets with various sizes
experiencing wide trajectories and drying profiles
(Masters, 1991), the spray-dried particles are almost
always polydisperse (often existing as aggregates) with
non-uniform shapes and morphologies (Kortesuo et al.,
2002). These issues lead to a lack of repeatability in terms
of the particles’ release behaviours, rendering it difficult to
correlate the physicochemical properties of the particles to
their functionalities, including dissolution and release
Address for correspondence: Cordelia Selomulya, Department of Chemical Engineering, Monash University, VIC 3800, Australia. Tel: þ61 3 99053436.Fax: þ61 3 99055686. E-mail: [email protected]
(Received 9 Nov 2011; accepted 12 Mar 2012)http://www.informahealthcare.com/mnc
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(Wang et al., 2006; Liu et al., 2011a). Hitherto, the influ-
ences of microstructural and morphological properties on
the controlled release properties for spray-dried particles
have largely been ignored, although they could be the
limiting factors in the design and quality control of phar-
maceutical products, as well as the scale up of the
manufacturing process (Sollohub and Cal, 2010).
The microfluidic jet spray drying technique developed
in our laboratory at Monash University can produce highly
monodispersed (non-aggregating) microparticles with
excellent uniformity, and is able to handle a variety of
multi-components precursors (Amelia et al., 2011;
Fu et al., 2011; Wu et al., 2011a). In the past, we have uti-
lized the technique to investigate the release behaviour of
polymeric particles prepared with different solvents
(Liu et al., 2011a), the effects of different dopants on
silica-based microencapsulates (Wu et al., 2011c) and the
formation of core-shell polymer/silica particles (Liu et al.,
2011b). Previously, the control over the functionalities of
particles was mainly realized from the choice of the initial
excipients materials. Currently, we set to investigate the
impacts of the manufacturing process, including drying
temperatures and pH of the precursors on the release
behaviour of the particles.
We employed the same technology here to tailor the
properties of drug-loaded microencapsulates by adjusting
the manufacturing parameters for particle formation.
Silica, as a common carrier matrix with easily tunable prop-
erties and good biocompatibility, was used as the excipient,
while lactose was also used in some of the particles to
modify their properties. Two different model drugs (rhoda-
mine B (RhB) and chromotrope 2R (ChR)) were used to
study the effects of the interactions between drug mole-
cules and the carrier matrix on the release behaviour.
Both have been used for encapsulation in various matrices
(e.g. silica, polymer and organic/inorganic hybrid) in their
stable forms to study controlled release properties (Fujii
et al., 1990; Anderson et al., 2001; Xu et al., 2008). The
outcomes should enable the development of a guideline
to fabricate silica-based pharmaceutical particles and
other spray-dried functional particles, by providing infor-
mation on the likely impacts of the precursors’ composi-
tions and drying conditions on the particle properties, thus
reducing the requirement for trial and error experiments in
conventional spray drying.
Materials and methods
Materials
Tetraethoxysilane (TEOS, �99.0% (GC), molecular
weight¼ 208.33 g/mol), lactose (alpha-D-lactose monohy-
drate), RhB, ChR and phosphate-buffered saline (PBS,
pH 7.4) consisting of 0.138 M NaCl, 0.0027 M KCl, 0.01 M
Na2HPO4�2H2O and 0.00176 M KH2PO4, were purchased
from Sigma–Aldrich (NSW, Australia). Hydrochloric acid
and sodium hydroxide were from Ajax Finechem (NSW,
Australia) and Merck (VIC, Australia), respectively.
Deionized water (Milli-Q) was used for all precursor
preparation.
Precursor preparation
The compositions of precursors for spray drying are listed
in Table 1. In a typical procedure, 22.29 mL TEOS (equals to
6.0 g silica dioxide) was added to 20 mL of 0.1N HCl solu-
tion with continuous stirring at room temperature. The
acidic hydrolysis was performed for 30 min to obtain a
homogeneous transparent sol. The sol was diluted with
deionized water to certain volumes for specific concentra-
tions of silica (e.g. the certain volume of 200 mL was used
for a 3.0% silica sol). Then 200 mL of the diluted silica sols
was taken and the pH value was adjusted to pH 2 or 5 using
HCl or NaOH. Calculated amounts of lactose and either
RhB or ChR as the model drug were added into the sil-
ica sols according to the desired ratio. The amount of
model drug was kept constant at a drug-to-matrix ratio
of 1/20 (w/w), giving a maximum theoretical drug loading
of 4.76 wt% within the final spray-dried microencapsulates.
The theoretical drug loading was calculated according to
the following equation:
Theoretical drug loading ð%Þ
¼Weight of drug � 100
Weight of drug þWeight of matrix
Table 1. Compositions of the precursors for spray drying.
Run
number
Silica
(% w/v)
Lactose
(% w/v)
Model
drug (w/w)
Precursor
pH
Inlet
temperature (�C)
Particle
size (mm)
Encapsulation
efficiency (%)
1 3.0 0.0 1/20 RhB 2 146 55.30� 1.94 98.14� 3.86
2 2.5 0.5 1/20 RhB 2 146 50.78� 4.97 98.01� 2.79
3 1.5 1.5 1/20 RhB 2 146 56.71� 2.91 95.11� 3.41
4 0.5 2.5 1/20 RhB 2 146 55.29� 3.57 99.08� 4.31
5 5.0 5.0 1/20 RhB 2 146 82.26� 6.71 94.05� 1.02
6 5.0 5.0 1/20 RhB 2 200 81.57� 5.80 95.24� 1.56
7 5.0 5.0 1/20 RhB 2 235 83.01� 2.15 94.24� 2.59
8 1.5 1.5 1/20 ChR 2 146 55.24� 2.31 97.56� 2.37
9 1.5 1.5 1/20 RhB 5 146 58.29� 4.07 96.70� 3.51
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Particle fabrication
The preparation of monodisperse microencapsulates was
done by a microfluidic jet spray drying technique as
described by a previous study (Wu et al., 2011a). Briefly,
the precursor solutions were fed into a standard steel res-
ervoir and dehumidified instrument air was used to force
the liquid in the reservoir to jet through the nozzle (orifice
diameter: 75 mm). The liquid jet was broken into droplets
by disturbance from vibrating piezoceramics. The droplet
formation mode was monitored by a digital SLR camera
(Nikon, D90) with a speed-light (Nikon SB-400) and
a micro-lens (AF Micro-Nikkor 60 mm f/2.8D). The liquid
flow rate and applied disturbance frequency were
adjusted to achieve best monodisperse droplet formation
(Wu et al., 2011b). The frequency of disturbance from
vibrating piezoceramics was kept at 12 000 Hz and the
liquid flow rate was tuned to be in the range of
1.70� 0.50 mL/min for all precursors. These monodisperse
droplets obtained were well dispersed and dried at specific
inlet temperatures.
Particle characterization
Images of microencapsulates were recorded by light
microscopy (Motic B1-223A, UK). Particle size and
size distribution were analysed using the software package
Motic Images Plus 2.0 ML and ImageJ. At least 500 parti-
cles were measured and analysed for each sample. The
morphology and microstructure of microencapsulates
before and after the release test were characterized
by field-emission scanning electron microscopy
(FESEM, JEOL 7001F, Japan). Elemental distribution
maps on particle cross-section were conducted by the
energy-dispersive X-ray analysis (equipped on the
FESEM, JEOL 7001F).
In vitro drug release test
In a typical experiment, microencapsulates (25 mg) were
weighted into a 100 mL conical flask, and 50 mL of PBS
release medium (pH 7.4) was transferred into the flask.
The flask was kept in a shaking incubator at 37�C with
constant agitation (100 rpm). At certain time intervals,
1 mL of the release medium was withdrawn from the
flask and replaced with the same amount of fresh release
medium. Collected samples were transferred into 1.7 mL
microtubes, centrifuged for 5 min at 10 000 rpm (Heal
Force, Neofuge 23R) and subjected to assay immediately.
The content of model drug in the sample was determined
by a microplate reader (SpectraMax M2e, Molecular
Devices) at the wavelength of maximum absorbance
(555 and 510 nm for RhB (Ignace, 1983) and ChR
(Han et al., 2011), respectively). Both experiments and
measurements of absorbance were done in triplicate.
Drug encapsulation efficiency
The total amount of model drug encapsulated into micro-
encapsulates was determined by dissolving an accurately
weighed amount of microencapsulates in 10 mL of 5%
NaOH solution. After the dissolution of particles, the solu-
tions were centrifuged for 5 min at 10 000 rpm and the
amount of model drug in supernatant was determined by
the microplate reader under the maximum absorbance.
The encapsulation efficiency of model drug was calculated
by dividing the amount of model drug encapsulated by the
theoretical amount of model drug (calculated from the
amount of model drug added during the manufacturing
process). Both experiments and measurements of absor-
bance were done in triplicate.
Results and discussion
The average particle size and drug encapsulation efficiency
of the final spray-dried particles are summarized in Table 1.
The encapsulation efficiency in microencapsulates was
nearly 100% of the initial drug added in the precursors in
all cases. This is desirable when dealing with valuable
ingredients in pharmaceutical and bio-related applica-
tions, as it reduces the amount of compounds that could
be lost due to poor encapsulation or from washing or sep-
aration steps. The high encapsulation efficiency was due to
the effective drying process, where each droplet was con-
verted into individual particle with virtually no waste. The
encapsulation efficiency was significantly higher than those
attainable by wet chemistry-based methods, where the
encapsulation efficiency was 550% in many cases
(Acharya et al., 2010a; Ito et al., 2011).
Effects of silica/lactose ratio on release rate
The release profiles of RhB from the spray-dried microen-
capsulates with different silica/lactose ratios are shown in
Figure 1. Pure silica microencapsulates (Run 1) showed a
very slow release rate with a cumulative release of around
10% after 48 h. Incorporation of lactose significantly
enhanced the release rate, even at the lowest concentra-
tion. Around 70% of drug was released within 48 h for
microencapsulates with 0.5% lactose (Run 2), while 80%
was released in the same period with increasing amount
of lactose added. Incorporation of lactose increased the
release rate due to its high hydrophilicity and water solu-
bility (Wu et al., 2011c). Based on our previous study,
lactose has been shown to be an effective ingredient to
boost the drug release rate (Wu et al., 2011c). By studying
the impacts systematically here, we could elucidate the
cause of the phenomenon of accelerated release from
adding lactose to the carrier matrix. Scanning electron
microscope (SEM) images of microencapsulates composed
of pure silica (Run 1) and different silica/lactose ratios
(Runs 2–4) are shown in Figure 2. All particles showed
relatively similar size and morphology with nearly spherical
Spray drying of monodispersed microencapsulates 3
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shape and relatively wrinkled surface, such that the differ-
ence in the release behaviour observed here could be
attributed solely to the addition of lactose to the matrix at
different ratios. An example of elemental distribution for
particles produced in Run 3 confirmed the relatively homo-
geneous distribution of silica and lactose (shown as
‘‘carbon’’) across the entire particle (Figure 3). As a soluble
filler, dissolution of lactose upon contact with a release
medium would provide more channels for drug diffusion,
inducing more drug molecules to be released at a given
time interval (Sinha et al., 2011). Different extents of parti-
cle degradation after the release test could be clearly
observed from the SEM pictures in Figure 4, consistent
with the observed release behaviour. In the absence of lac-
tose, only a small number of sub-micron pores were
observed after the release test, possibly due to silica erosion
0 10 20 30 40 50
0
20
40
60
80
100
Cum
. Rel
ease
(%
)
Time (h)
3.0% Silica/ 0.0% Lactose 2.5% Silica/ 0.5% Lactose 1.5% Silica/ 1.5% Lactose 0.5% Silica/ 2.5% Lactose
Figure 1. Release profiles of microencapsulates with different silica/lactose ratios.
Figure 4. SEM images of microencapsulates after the release test:
(A: Run 1; B: Run 2; C Run 3; particles of Run 4 were not shown because
the particles were completely disintegrated).
Figure 2. SEM images of uniform spray-dried microencapsulates from:
Run 1 (3.0% silica/0.0% lactose); Run 2 (2.5% silica/0.5% lactose); Run 3
(1.5% silica/1.5% lactose); and Run 4 (0.5% silica/2.5% lactose) (inset scale
bar: 10 mm).
Figure 3. Elemental distribution maps of the cross-section of microen-
capsulates from Run 3 (1.5% silica/1.5% lactose).
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(A2 in Figure 4). However upon addition of lactose in the
matrix, relatively loose texture and large cracks were found
on the surface of the particles after release, possibly due to
the dissolution of lactose that also enhanced buffer pene-
tration into the particles (B2 and C2 in Figure 4). Hence,
lactose could be a useful regulator in tuning the speed of
drug liberated from microencapsulates.
Effects of drying temperature
Drying temperature is an important parameter in spray
drying, influencing both the products properties and the
production costs. Generally, a low drying temperature is
preferred during the production of pharmaceutical parti-
cles to conserve the properties of the active ingredients.
However, the practical drying temperature should also be
at the level where complete drying of the initial atomized
droplets could be achieved to obtain a reasonable produc-
tion yield in a short period. Here, we investigated the effects
of three inlet temperatures (146�C in Run 5, 200�C in Run 6
and 235�C in Run 7) on a specific formulation (5% silica/5%
lactose with RhB) to understand the influence of different
drying temperatures on particle properties. The tempera-
ture range was chosen to reflect the conditions that parti-
cles may experience in a typical spray drier for aqueous
solutions (He et al., 1999). The drying temperature
showed a noticeable impact on the morphology of the par-
ticles (Figure 5). High drying temperature resulted in
spherical microencapsulates with smooth surface, while a
low drying temperature led to nearly spherical particles
with rough surface. Particles dried in between these tem-
peratures showed the transition states in both shape and
surface features.
This change in particle morphology was caused by dif-
ferent drying rates (Tonon et al., 2008). Higher drying tem-
perature results in faster drying/solvent evaporation rate,
and leads to a quick formation of a smooth and hard crust
with little time to deform and form wrinkles, whereas a
slower drying rate could enhance the surface roughness
with more time to form the shell (Maa et al., 1997;
Mezhericher et al., 2010). Figure 6 showed the release pro-
files with a slightly slower release rate for the smooth par-
ticles formed at higher drying temperature. Since the
particles were relatively of the same size, the wrinkled sur-
face for lower temperature spray-dried particles could
increase the contact area with the buffer and promote a
faster release rate (Lamprecht et al., 2003).
Effects of drug type
Different model drugs (RhB/ChR with detailed physico-
chemical properties as presented in Table 2) were added
to precursors with the same compositions. The two model
drugs have very similar molecular weights and are both
highly water-soluble. The major difference is that after ion-
ization, RhB is positively charged while ChR is negatively
charged. Figures 7 and 8 displayed the SEM photos and
release profiles of microencapsulates spray-dried with
RhB (Run 3) and ChR (Run 8). The particles containing
ChR formulation had a relatively faster initial release rate
than those containing RhB.
The drug release from the matrix should be dependent
on several factors: concentration gradient of the drug,
surface area and diffusion coefficient (Acharya et al.,
2010b). Since the molecular weights of the drugs and the
drug loadings were similar with the same matrix formula-
tion, we could assume that the concentration gradient of
each drug was comparable. In addition, the minor differ-
ence in surface roughness (Figure 7) and the similar parti-
cle size implied equivalent surface areas of the particles.
Hence the difference on the initial release kinetics was
primarily caused by the diffusion coefficients due to
drug–matrix interactions. For silica-based matrix materials,
the release barriers consist of tetrahedral latticed siloxane
units with the silanol functional groups on the surface
(Wu et al., 2004). The protonation or deprotonation of
the silanol groups is dependent on the solution pH, with
the negative charges on the silica surface remaining low
Figure 5. SEM images of microencapsulates spray-dried at different inlet drying temperatures: (A) Run 5 (146�C); (B) Run 6 (200�C); and (C) Run 7 (235�C).
0 10 20 30 40 50
0
20
40
60
80
100
Cum
. Rel
ease
(%
)
Time (h)
0 1 2 3 4 50
20
40
60
80
Run 5_146oC Run 6_200oC Run 7_235oC
Figure 6. Release profiles of microencapsulates spray-dried in Runs 5–7
(under different inlet temperatures).
Spray drying of monodispersed microencapsulates 5
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until the solution pH reaches 6, and then increasing sharply
between pH 6 and 11 (Atkin et al., 2003). Since the release
test was performed at pH 7.4, there should be relatively
strong electrostatic repulsions between ChR and the silica
matrix especially at the initial stage (Burke and Barrett,
2004), with the rate decelerating at the later stage when
there was less drug available for release (thus reducing
drug gradient for diffusion).
Effects of pH of precursors
It is well known that the hydrolysis process of silicon oxides
is accompanied with a condensation process (Brinker and
Scherer, 1990). The rates of the hydrolysis and condensa-
tion are dependent on the environmental pH values and
can influence the final structures of the formed silica mate-
rials (Brinker, 1988). The condensation of silica species in a
strong acid solution is at a minimum for the pH from 1.5
to 2, which is near to the isoelectirc point (IEP), and at a
maximum for pH ranging from 6 to 7 (Cihlar, 1993). Thus,
pH 2 (near IEP) and 5 were selected here to investigate the
influence of pH values while still maintaining stable solu-
tions for the microfluidic jet spray drying. Precursors with
the same compositions but different pH values were spray-
dried under the same conditions (pH 2 for Run 3 and pH 5
for Run 9). The morphology of particles was shown
to be dependent on pH values, with particles spray-dried
from precursor with pH 5 showing more deformed shapes
than those at pH 2 (the 1st column in Figure 9). The release
profiles are shown in Figure 10, illustrating similar release
behaviours, with a slightly faster initial release rate for
those spray-dried at pH 5. Comparison of the states of par-
ticles after the release test (the 2nd column in Figure 9),
demonstrated that the particles from precursors at pH 5
showed more fragmented morphology, illustrating that
pH influenced the microstructures and consequently the
Table 2. Physicochemical properties of the two model drugs.
Name RhB ChR
Chemical structure
O
COOH
NCH3
N+
CH3
CH3
ClCH3
SONa
O
OS
ONa O
O
N
N OH OH
Molecular weight 479.01 g/mol 468.37 g/mol
Water solubility Very soluble: �50 g/L Very soluble: 4100 g/L
Figure 7. SEM images of microencapsulates spray-dried with different model drugs: Run 3 (model drug: RhB) and Run 8 (model drug: ChR) (inset scale
bar: 1 mm).
0 10 20 30 40 50
0
20
40
60
80
100
Cum
. Rel
ease
(%
)
Time (h)
0 1 2 3 4 5
0
20
40
60
80
100
Run 3_RhB Run 8_ChR
Figure 8. Release profiles of microencapsulates spray-dried from Runs
3 and 8.
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initial release rate. The effects of processing pH could be
explained by the stabilities of silica sols (Kortesuo et al.,
2002). For sols of hydrous oxides, the repulsive barrier
could be adjusted by tuning the charge-determining ions
Hþ and OH� (i.e. pH) with the colloids usually aggregating
around the IEP (Brinker and Scherer, 1990). However in the
unique case of silica (Mou and Lin, 2000), a hydration layer
around the silica species influences the surface properties,
making the interactions among the silica species only
weakly attractive and preventing the coagulation at IEP
(Horn, 1990). It has been reported that the degree of aggre-
gation of oxide species in a sol precursor could affect the
structure of the spray-dried particles (Sizgek et al., 1998),
with the densest particles actually produced by silica sols at
the IEP (Kortesuo et al., 2002).
Conclusion
Particulates with predefined specifications are crucial in
the manufacturing and development of pharmaceutical
products. By generating uniform particles, we could sys-
tematically investigate the influence of formulation and
process parameters on the production of silica-based
microencapsulates for controlled drug release. The addi-
tion of a small molecular disaccharide (lactose) signifi-
cantly altered the microstructure of the particles, and
resulted in significantly faster release kinetics. The surface
features of the particulates were affected by the drying tem-
peratures, while the physicochemical properties of the
model drugs showed moderate influence on the release
properties. The results demonstrated a degree of control-
lability over particle properties and controlled release
behaviours through formulation and manufacturing pro-
cesses and the knowledge would be useful in designing
silica-based pharmaceutical particles for specific
applications.
Acknowledgements
Wenjie Liu would like to acknowledge Monash University
and China scholarship council for providing the collabora-
tive PhD scholarship.
Declaration of interest
The authors report no conflicts of interest. The authors
alone are responsible for the content and writing of the
article.
Figure 9. SEM images of the microencapsulates spray-dried from precursors with different pH values.
0 10 20 30 40 50
0
20
40
60
80
100
Cum
. Rel
ease
(%
)
Time (h)
0 1 2 3 4 5
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20
40
60
80
Run 3_pH=2 Run 9_pH=5
Figure 10. Release profiles of microencapsulates spray-dried from Runs
3 and 9.
Spray drying of monodispersed microencapsulates 7
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