Sonia Wallman, PhDNBC2

swallman@biomanufacturing.org

Yakov Cherner, PhDATeL, LLC

ycherner@atelearning.com

Web-Based Virtual and Hybrid Laboratories for Biomanufacturing Curriculum

BIOMAN

Laboratory

Textbook

Online Support

Online M

anualsCom

plet

e Su

ite o

f Pro

duct

s

Fall 2010

Global BioManufacturing Curriculum

Virtual Labs

Introduction to Biomanufacturing

Textbook • Authored by Industry Experts • 11 chapters plus introduction• Currently being piloted by

two Biomanufacturing classes, 37 Students total, and by 3 instructors teaching Biomanufacturing

• Industry interest• Scheduled for release, fall

2011

Introduction to Biomanufacturing

Engineering• Operational

Excellence Marti Mathews, Biogen

Idec

• Facilities Brian Shingle, Centocor

• Metrology Gene Kernan, HGSI

• Validation Patrick Sheehy, HGSI

• EH&S John Piampiano, Kodak

Production• Upstream Processing Olga Mollin, Jane Flanagan, Lonza

• Downstream Processing

Paul O’Neil, Pall Euroflow

• Process Development Ezster Birck Wilson

Quality• Quality Assurance Cynthia Sarnoski, Frank Hallinan, Pfizer Ron Hudcosky, Merck

• Microbial Control John Hasyn, MCCC

• QC Biochemistry Mary Harne, Zhenhong

Lee., HGSI

Biofuels Curriculum

Biofuels Textbook

Biofuels Lab Manual

Laboratory Manuals

Generic Custom CHO – tPA Pichia pastoris - HSA Protein is Cash

Generic Laboratory Manual

Metrology: SOPs for equipmentValidation ProtocolsEH&S ProgramQuality Assurance: cGMP, SOPs, Batch

Records, Equipment LogsQuality Control Microbiology SOPsUpstream Processing: E. coli recombinant for

GFP, CHO recombinant for tPA Cell Culture,

Pichia pastoris recombinant for HSA Cell Culture

Downstream Processing: Purification of human tPA from CHO Cell Culture

Purification of human serum albumin from Pichia pastoris Cell Culture

Quality Control Biochemistry(Process Development)

NBC2 Biomanufacturing Laboratory Manual Adopters

• Mount Wachusett Community College (BMS is biomanufacturer partner)

• Minuteman Regional High School Adult Evening Program (Shire is biomanufacturer partner)

• Hudson Valley Community College (Regeneron is biomanufacturer partner)

• Great Bay Community College (Lonza and Stryker are biomanufacturer partners)

• Montgomery County Community College• Hagerstown Community College

‘Protein is Cash’ lab manual

Overview of Protein is Cash Manual – Metrology– Transformation– Upstream processing– Downstream processing– Qualitative analysis– GMP/Food and Drug Analysis Can be found at: www.biomanufacturing.org

‘Protein is Cash’ :Adult Certificate Program

Biomanufacturing Certificate Program • Derived from Protein is Cash and Global Manufacturing Curriculum • 12 week course• 2 hours of lectures, 6 hours of laboratory

activities per week

The Virtual Chromatography Laboratory on the Web

URL: http://ATeLearning.com/BioChrom/

Password: hi-tec2011 (lower case)

Motivation for developing virtual biomanufacturing laboratories:

• Provide students of digital generation with visual instructions and learning tools that more relevant to their every-day habits and experience than traditional printed materials

• Enhance hands-on training

• Supplement and/or substitute for hands-on training (high school students, where time in the lab is limited, and e-learners)

• Substitute for or enhancement of printed instructions for hands-on experiments (e.g. - textbooks and user’s manuals may not be necessary)

• Shorten training time for students and employees

• Facilitate just-in-time learning to enhance students’ understanding and to create transferrable skills and knowledge.

• Provide technology and tools for performance-based assessment.

Simulation-based Virtual Learning Environment

Simulations

Virtual experiments (self-guided online assignments for students)

Assessment tool

Embedded resources for “just-in-time” learning (Online lessons, presentations, papers, etc.)

Auxiliary resources (glossary, animations …. )

Virtual experiment focuses on a particular task, students have to follow guidelines Includes:• main simulation• specific learning objectives• scenario/assignment• step-by-step instructions for students• worksheet • assessment

optional components:auxiliary simulations, prerequisites, excerpts from interactive lessons andtechnical manuals, quizzes, and other onlinereference resources.

Simulation task and learner neutral, there are no restrictions on

user’s actions

Target audience:

Teachers and instructors Students

Simulations vs. Virtual Experiments

Includes:• brief theory• help

Comprehensive Learning/Teaching Environment

Traditional printing curriculum resources (textbooks, user’s manual, worksheets, etc.)

Hands-on laboratories

Virtual laboratories

Remote laboratories

Hybrid (blended) laboratories (hands-on & virtual labs, remote & virtual labs)

On-line curriculum resources

Auxiliary tools (authoring tools for customization of virtual labs and online resources, communication tools (forums, blogs, e-mail, etc.) learning management tools)

LP Biologic Chromatography Lab

Introduction:

Goal: Introduce equipment and to study the design and functionality of main system components.

Operation: (auto and step-by-step):

Goal: Demonstrate the system operation and major processes.

Virtual experiment:

Goal: Facilitate an online experiment that exactly match experimental tasks that students face in workplace or in actual college labs.

Assessment

Goal: Evaluate students’ knowledge and skills

APPLICATION MODES:

Run Demo

Password: hi-tec2011

The proposed blended learning cycle recommends a five-step approach to student learning:

Learning cycle

Use simulations and online materials to learn the theory and basic principles underlying protein purification;

Use simulations to become familiar with a liquid column chromatography system, its components and their function;

Perform virtual experiment(s);

Evaluate knowledge and virtual skills, using a built-in assessment tool, before granting access to the real chromatography system;

Use the real BioLogic® LC Chromatography System to purify proteins.

Future plans:

Developing new virtual experiments;

Completing the major blocks of this Virtual Liquid Chromatography Laboratory (i.e., Lessons, Assessment, Glossary)

Creating virtual labs and relevant e-learning materials for other subjects;

Thorough testing to evaluate and compare the impact on student learning of hybrid labs versus the separate use of hands-on or virtual labs;

You are invited to participate in piloting our Virtual Liquid Chromatography Lab.

URL: http://www.atelearning.com/BioChrom

Password: hi-tec2011 (lower case)

We believe that the combination of online and hands-on learning ensures integration of theoretical knowledge and practical skills and enhances students’ understanding and workplace performance.

Dr. Yakov Cherner

ATeL, LLCPhone: (781) 842-3300e-mail: YCherner@ATeLearning.comWeb site: http://ATeLearning.com

Contact:

Dr. Sonia Walman

NBC2

e-mail: swallman@biomanufacturing.orgWeb site: http://Biomanufacturing.org