[ LCT PREMIER XE ]

Solvent free extraction, fractionation, and isolation of chemical compounds

Superior purity, selectivity, and yield. Lower operating costs.

[ SuPERCRITICaL FLuId EXTRaCTIon SySTEMS ]

Faster and cleaner technology to keep your product safe, uncontaminated, and natural.

The demand for analytical laboratory

techniques is driven by the need

for enhanced information about

a target material, including its

chemical, physical, nutritional,

and pharmaceutical properties.

Increasing concern about the quality, safety, usage,

and disposal of hydrocarbon-based solvents, as

well as stricter regulations on the residual levels

of solvents have all contributed to many industries

switching to SFE technology for the extraction,

fractionation, and isolation of chemical compounds.

Waters® Supercritical Fluid Extraction (SFE) Systems

extract chemical compounds using CO2 in its super-

critical state in place of organic solvents. The result is

[ SuPERCRITICaL FLuId EXTRaCTIon SySTEMS ]

n Environmentally friendly

n Lower operating costs

n Little to no residual solvent

n Processes thermolabile compounds

n Safe

n Scaleable

Faster and cleaner technology to keep your product safe, uncontaminated, and natural.

an extract with little or no residual solvent, superior

purity, selectivity, yield, and lower operating costs

compared to traditional hydrocarbon-based

solvent extraction systems. SFE provides a faster,

safer, and cleaner technology for analysts from the

food, natural product, flavor, fragrance, pharmaceutical,

nutraceutical, polymer, chemical, and cleaning

industries, as well as regulatory agencies and

quality control laboratories.

Green Technology

Waters SFE systems extract chemical compounds using

supercritical CO2 instead of an organic solvent. The

supercritical fluid state occurs when a fluid is above its

critical temperature (Tc) and critical pressure (Pc). This

supercritical state allows CO2 to take on the properties

of a gas (high diffusivity, low surface tension), as well

maintaining the solvent power of a liquid when it is

between the typical gas and liquid states. Manipulating

the temperature and pressure of CO2 alters the solvent

power and allows the material of interest to be

selectively extracted.

The biggest advantage of SFE is that it leaves no traces in

the product. After extraction, the carbon dioxide is either

depressurized and vented, or it can be recycled for further

extraction use. Any residual trace of carbon dioxide in the

product dissipates into the atmosphere within a few hours.

As a tunable solvent, CO2 is non-toxic, non-flammable, and

physiologically compatible.

The biggest advantage of SFE is that you can extract your compound with little or no residual solvent, leaving no traces in your product.

(Above) Tea and coffee are decaffeinated via supercritical fluid extraction. (Center) Most major brewers in the US and Europe use flavors that are extracted from hops using SFE Technology. (Right) More concentrated extracts of St. John’s Wort are obtained through SFE than with conventional technologies.

Ideal tool for a range of applications

SFE is a selective technique that is capable of extracting

compounds from solid maticies to isolate the analyte of

interest. Hydrocarbon-based solvent extraction requires a

distillation/dry down step that may alter the taste, aroma,

and/or chemical composition of the product. Many other

applications have been fully developed and commercialized

incorporating the SFE technique, including:

n Extraction and fractionation of edible fats and oils

n Purification of solid matrices

n Separation of tocopherols and other antioxidants

n Cleanup of herbal medicines and food products from pesticides and herbicides

n Detoxification of shellfish

n Concentration of fermentation broth, fruit juices, etc.

n Flavors, spice extracts, herbs, decaffeination

n Fragrances

n Natural food colors

n Desolvation within tablets

n Dietary supplements, such as St. John’s Wort, saw palmetto, kava-kava, ginger, garlic, and ginseng

n Production of denicotined tobacco

n Production of active ingredients from herbal plants for avoiding thermo or chemical degradation

n Active compounds of all kinds, including steroids and polymer/monomer separation

n Precision parts cleaning

[ Supercritical Fluid extraction SyStemS ]

BLEED VALVE

DRAIN VALVE

ON/OFF VALVE

GAUGE

GAUGE

AUTOMATEDBPR

MANUALBPR

FLOW METER

MIXER

COOLINGHEAT

EXCHANGER

HEAT JACKET EXT RACTIONVESSEL

HEATEXCHANGER

COOLING BATH

CO2 PUMP

CO SOLVENT PUMP

VENT

CO-SOLVENTRESERVOIR

CO2 SUPPLY IN

FRACTIONCOLLECTOR

HEAT JACKET

By manipulating the pressure and temperature, CO2 can

selectively extract the desired material. The sample is

placed in an extraction vessel and pressurized with CO2

and a small percentage of co-solvent to extract the

compound of interest. This compound is then transferred

from the extraction vessel to a fraction collector. The

automated Back Pressure Regulator (BPR), located between

these vessels, allows for controlled depressurization of

the compounds of interest and the CO2. After exiting the

automated BPR, the system pressure is reduced to near

atmospheric conditions, causing the CO2 to lose its

solvating power. The extracted material precipitates out

of the solution into the fraction collector. The condensed

CO2, now a gas, is sent to vent, or it can be recycled.

Extract compounds without altering the taste, aroma, or chemical composition of your product

Waters SFE Systems include a PC with custom software

for instrument control and data management. Regardless

of your native language, the comprehensive, yet simple

icon-based interface is easy to use. The user controls the

pressure, temperature, flow rates, as well as data logging,

data mining, and the ability to write scripts

tailored to your application.

How

Major system components: High pressure CO

2 pump

Known for quality, Waters’ dual-piston CO2

pumps are used by academic and corporate

research scientists in over 40 countries.

This pulseless flow pump requires no air,

operates quietly, and can be used in a

pressure-control or flow-control mode.

Saftey features include mechanical and

electrical ovepressure protection.

High pressure vessel

The finger-tight high pressure vessel

is designed for simple opening and

closing, and even distribution of the fluid

contacting the sample. A threaded cap

with a spring-loaded seal enhances safety

and enables effortles sample loading and

unloading. Waters’ unique stainless steel

is 60% stronger than traditional 304 and

316 stainless steel, which minimizes heat

up and cool down times All vessels are

designed per the ASME pressure code and

CE code if applicable.

Automated Back Pressure Regulator (BPR)

Our state-of-the-art BPR features a

pressure sensor for closed-loop feedback

control of the back pressure, and built-in

heating to prevent freezing.

Easy-to-use software tailored to your application

[ SuPERCRITICaL FLuId EXTRaCTIon SySTEMS ]

Custom software provides easy-to-use instrument control and data management.

High pressure collection

A Waters cyclonic-style collection separator can collect

particles or liquids from gases or liquids. Collection starts

with fluid being introduced at high velocity into the fraction

collector, where centrifugal forces press heavier particles

against the inner wall of the separator, while the lighter

particles exit through the tapered bottom of the fraction

collector. The self-sealing, finger-tight cap is easy to

open and close.

Benchtop to Cart- Mounted Systems Ranging from 100 CC to 2 X 5000 CC

Waters’ benchtop to cart-mounted

systems are pre-assembled and

ready to install. Customer training

courses are available on-site, or at

our training facilities in the US or UK.

n SFE 100

n SFE 500

n SFE 1000

n SFE 2000

n SFE 5000

n SFE 2 X 5 LF

System options include:

n CO2 Flow Meter

n Multiple Extraction Vessels

n Multiple Fraction Collectors

n Recycle System

n Counter Current Column

SFE 1000 System.

Waters, is a registered trademark of Waters Corporation. The Science of What’s Possible is a trademark of Waters Corporation. All other trademarks are the property of their respective owners.

©2010 Waters Corporation. Printed in the U.S.A.October 2010 720003629EN AO-CP

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