SOCOSCOPE - · PDF filesealing baseplate. ... Fundamentals of Membrane Encapsulated Cell and...

October 2003 | www.socochim.ch

In this issue :

Separat ion techniquesAlltech 2-3Spectrumlab 4-5

Life sciencesKPL 6Genotech 7Biowest 8-9Ken-En-Tec 10-11Molecular Biosciences, Inc 12-13Seegene 14-15Genotech 16-17Minerva 18American Dignost ica 18Pierce Chromatigraphy 19

LabwareBerthold 20Alltech 21ABgene 22RBS 22La-Pha-Teck 23ILS 23

Dear Customers,

Since 1986 Socochim SA has been pleased to supply youwith products, amongst others, from Alltech, Perbio, andSpectrum for your laboratory analyses and researches.

Upon receipt of our latest booklet, you may have noticedour new corporate identity that we implemented at thesame time that we extended our offer to the products offourteen “new” Companies that we now represent inSwitzerland.

Most of theses “new” Companies do not produce publicitymaterial such as catalogs and flyers, since all the infor-mation can be found on their respective Web sites. Withthe objective of keeping you informed of their latest devel-opments and high quality products we have decided topromote them with a quarterly flyer that we will be send-ing to you. An additional E-Newsletter will also be sent toall customers willing to receive it.

As you can see the management team of our Company isstrongly motivated to bring added value to your work.

Please feel free to ask about our services.

Best regards,

Dr. Patrice HumbertVP, Business Development

For information and order :

Socochim SA

Ch. du Trabandan 28

CH-1006 Lausanne

Phone +41 (0)21 721 04 50

Fax +41 (0)21 721 04 51

[email protected]

www.socochim.ch

S O C O S C O P E

magazine Socochim 13/10/03 16:55 Page 2

Separat ion Techniques

2

• Remove impurities to deliver99.9999% pure gas

• Exchange filters in seconds, withoutintroducing air into the system

• Diffusion-proof with glass and metalconstruction

• Pressure resistant up-to 150psi(11bar)

• Remove hydrocarbons to deliver99.9999% pure gas

• Improve MSD S/N ratio up to 10-fold

• Purify up to 20L/min nitrogen • Install cartridges quickly and

easily without the use of tools

Use SGT’s LC/MS filter on yourNitrogen line to deliver 99.9999% puregas. Flow is split within a 2-positionbaseplate so that half gets sent toeach high surface area charcoal trap,and then is rejoined at the exit of thebaseplate (see Figure 1). These car-tridge-style filters maintain the “fast-connect” principle which means they“snap” onto the self-sealing basepla-te. The glass housing is diffusion-proof and pressure resistant to 150psi(11bar).

Install and replace cartridges in seconds without shutting down LC/MS.High Surface area charcoal removeshydrocarbons to 0.1ppm.

SGT Super-Clean® Filters for LCDescription Part No. Price CHF

LCMS Baseplate

2-position baseplate B0021 921.-

Filter Cartridges

2-Hydrocarbon Filters F0720 547.50

Filter System (Cartridges + Baseplate)

2-Hydrocarbon + 2-positon baseplate B1021 1353.50

SGT’s Super-Clean Filters for Nitrogen Gas in LC/MSImprove Baselines, Minimize Interferences, and Reduce MS Maintenance

SGT’s Super-Clean Filters for GCImprove Baselines, Minimize Interferences, and Reduce MS Maintenance

N2

GasGenerator

LC/MSSystem

GAS IN

GAS OUT

Figure 1 – High N2 gas flow is split to parallel baseplates and then rejoined

Use SGT’s Super-Clean gas filters on allyour GC gas lines to deliver 99.9999%purity. These cartridge-style filtersmaintain the “fast-connect” principlewhich means they “snap” onto a self-sealing baseplate. Cartridges can beexchanged within seconds, during ananalysis, and without tools. 1-, 2-, or 3-

position baseplates are available and will accept any of the GC filtercartridges.

www.alltechweb.com


3

Triple Filter •One filter removes moisture, oxygen and

hydrocarbons •Color indicators for moisture and oxygen

saturation• Ideal for carrier gas lines•Standard Triple Filter is purged and shipped

with Argon (use with FID, ECD, NPD, TCD)•GC/MS Triple Filter is purged and shipped

with Helium (ideal for MSD)

Combi Filter • Removes moisture and

hydrocarbons• Color indicator for

moisture• Ideal for FID fuel gas lines

Hydrocarbon Filter • Packed with charcoal• Ultra-high capacity

SGT Super-Clean® Filters for GCDescription Part No. Price CHF

Baseplate

1-Position Baseplate B0010 457.-




Filter Cartridges

Triple Filter (Argon packed) F0301 314.50

Triple Filter (Helium packed) F0302 396.-

Combi Filter F0201 298.-

Moisture Filter F0101 278.50

Oxygen Filter F0102 278.50

Hydrocarbon Filter F0103 278.50

Filter Cartridge Bundles

1-Triple + 2-Combi F0730 875.50

Oxy + Moisture + 2-Hydrocarbon F0740 1075.50

Filter System (Cartridges + Baseplate)

1-Triple (Argon packed) + 1-positon Baseplate B1010 793.50

1-Triple (Helium packed) + 1-Postion Baseplate B1011 839.50

2-Combi + 2-positon baseplate B1020 1306.50

2-Moisture + 2-positon baseplate B1022 1287.-

1-Triple + 2-Combi + 3-positon baseplate B1030 2053.-

1-Oxygen + 1-Moisture + 2-Hydrocarbon + 4-position Baseplate B1040 2657.50

Oxygen Filter • Color indicator shows

saturation• Ultra-high capacity

Moisture Filter • Color indicator shows

saturation• Ultra-high capacity

www.socochim.ch – to order call 021 721 04 50 or fax 021 721 04 51

All our prices are in Swiss Francs excl. 7.6% VAT and shipping


Separat ion Techniques

4

Spectrum provides new technologydeveloped by the National CancerInstitute for screening of anti-cancercompounds. This in vivo method ofidentifying compounds with potentialchemotherapeutic activity againstcancer and HIV is now routinely usedby many researchers. The technologyallows human cells to be implantedinto host animals and subsequentlyretrieved in mass. While in the host,cells can be exposed to potentialchemotherapeutic compounds asantiviral agents and the impact of that

exposure can be measured afterretrieval. With this technology, targetcells are macroencapsulated in bio-compatible hollow fibers. Thesemacroencapsulated cells are thenimplanted into the subcutaneous orintraperitoneal compartments of lab-oratory mice. This methodology is asignificant step forward and results insignificant savings of time, labor,amount of compound required, andthe number of animals used in theevaluation of the potential therapeuticactivity of candidate compounds.

New from SPECTRUM®

CellMax® Implant Membrane for Drug Discovery and Development

Fundamentals of Membrane Encapsulated Cell and Tissue for Drug Discovery and Development

CellMax in vivo Models...What?Implants of human cells or tissue,which express the impact of animalmetabolism and excretion on thera-peutic efficacy and/or toxicity of anti-cancer/HIV agents.

CellMax in vivo Models....When?Screening agents for anti-cancer,anti-viral and antibiotic efficacy onhuman cell or organ toxicity andmolecular targeting.

CellMax in vivo Models...Where?Any laboratory facility and an animalmodel.

CellMax in vivo Models...Why?Provides rapid, accurate and inexpen-sive predictions of drug efficacyand/or toxicity with simple biosafemethodology.

CellMax in vivo Models...How?Implant cells or tissue, in muscleand/or intraperitoneal compartmentand dose animal with test compound.Retrieve cells and assay for viabilityand proliferation.

CellMax Implant MembraneHollow fiber implant technology for invivo drug profiling greatly reduces thecost and time of xenographic animalstudies while improving the sciencewith the use of human cell lines:

Screen compounds with the SpectrumCellMax hollow fiber in vivo assay.Compounds with poor pharmacokinet-ics are rejected and compounds withpro-drug activity are accepted.

Encapsulated cells can be:• Established cancer cell line

• Virus infected cells

• Hematopoietic cells

• Bacteria

• Fungi

CellMax isolates:• Immune systems cells

• Viruses

• Mycoplasma

CellMax has a:• Biocompatible inner and

outer surface

Advantages over current xenographic model:• 10 day assay versus 60 day assay

• Smaller assay variability, fewer ani-mals needed

• Uses small amounts of test com-pounds

• Multiple cell lines can be testedsimultaneously in same animal

• Compatible with different cell lines

www.spectrumlabs.com


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The hollow fiber assay is a unique invivo model permitting the simultane-ous evaluation of compound efficacyagainst six cell lines at subcutaneous(s.c.) and peritoneal (i.p.) sites(Hollingshead et al. Life Sci. 57;131, 1995). This model was used toinvestigate the relationship betweencell density and compound activity.Additionally, the model was adaptedfor mechanism of action studies. Thistechnology is now used routinely bythe National Cancer Institute of theNIH for testing compounds for anti-cancer activity. It is also beingemployed to look for antiviral com-pounds for HIV.

Specifications

Packaged Wet in DI water and Autoclaved

Material: PVDF

Inner Diameter: 1.0 mm

Outer Diameter: 1.2 mm

Length: 34 cm per fiber

Quantity: Three fibers per package

Tumor types and cell line applications in CellMax Implant membraneMany different cancer cell lines, including some poorly tumorigenic lines, have been grown in CellMax ImplantMembrane hollow fibers:

CellMax Implant Membrane Order Information

MWCO (Daltons) Product NumberWhite blue yellow green

500 kD, wet M138615 M1386161 M138617 M138618500 kD, dry S9320101 S9320102 S9320104 S932010Price CHF 175.- 175.- 175.- 175.-

Tumor Type Cell Line Tumor Type Cell Line Tumor Type Cell Line

Lung A549/ATCC Colon Colo-205 Lymphoid CCRF-CEMNCI-H23 HCC-2998 CEM-SS (HIV screen)NCI-H226 HCT-15 HL-60 (TB)NCI-H460 HCT-116 K-562NCI-H522 HT29 MOLT-4

SW-620 RLU937 (HIV screen)

Prostate DU-145 Breast MCF-7 Melanoma LOX IM VIJCA-1 MDA-MB-231 SK-MEL-5PC-3 MDA-MB-435 SK-MEL-28PC-3 (M) MDA-N UACC-62

UACC-257CNS SF-295 Ovarian IGROV1 Renal A498

SNB-75 OVCAR-3 CaKi-1U251 OVCAR-5 RXF-393

SK-OV-3 SN12CThe Spectrum CellMax Implant membrane in vivo model has shown positive effects, at both IP and SC sites, when using anticancer test compounds.

The membranes, made from modifiedPolyvinylidene Difluoride (mPVDF), repre-sent a revolutionary advance in separationtechnology. The membranes are biocom-patible, hydrophobic and resistant to awide variety of organic solvents, as wellas, most aqueous acids and bases. Themembranes can be heat sealed and auto-claved without affecting the molecularweigh cut off (MWCO) or changing themembrane performance.

CellMax Implant Membranes, the uniquepigmented hollow fiber with the ability toheat seal, is ideal for controlled release,cell culture, encapsulation and implanta-tion research. The MWCO is 500,000Daltons.

The surface of the CellMax Implant

Membranes has been shown to be biocompatible in various animal modelsfor periods exceeding 14 days.Multiple cell lines have been grown with-in the CellMax Implant Membranes bothin vitro and in vivo. Cell lines grown withinCellMax Implant Membranes, which havebeen implanted into a host animal, are notsubject to immunological attack by thehost animal. CellMax ImplantMembranes containing cancer cell lineshave been implanted into mice and usedto screen for compounds having anti can-cer activity. Similarly, CellMax ImplantMembranes containing HIV infected celllines have been implanted into mice andused to screen for compounds havinganti-HIV activity. Literature describing thismodel is available from Spectrum.

References1. Hall LA et al., Anticancer Research, 20(2A),

903-11 (2000).2. Bokesch HR et al., J Nat Prod. 62(4), 633-5 (1999).3. Xu Z et al., Bioorganic Medical Chemistr Letter,

9(2),133-8 (1999).4. Argade AB et al., Journal of Medical Chemistry,

41(18), 3337-46 (1998).5. Howard OM et al., Journal of Medical Chemistry,

41(13), 2184-93 (1998).6. Turpin JA et al., Antimicrob Agents Chemother,

42(3), 487-94 (1998).7. Buckheit RW Jr. et al., AIDS Res Hum Retroviruses,

13(9):789-96 (1997).8. Freije JM et al., Nat Med., 3(4):395-401 (1997).9. Hollingshead M et al., Antiviral Research,

28(3):265-79 (1995).10. Hollingshead MG et al., Life Science, 57(2):

131-41 (1995).11. Casciari JJ et al., Journal of National Cancer

Institute, 86(24):1846-52 (1994).




Life sciences

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Description

KPL was founded in 1979 to develop,manufacture and market high qualityimmunochemicals for life science andin vitro diagnostics. KPL was the firstcompany to commercialize affinitypurified secondary antibodies, a valua-ble tool for biological research and invitro diagnostic testing. Since then KPLhas introduced over 600 reagents andcomplete assay kits for protein visuali-zation and nucleic acid detection thataddress the needs of cell biology,immunology, protein chemistry andmolecular biology scientists.

Many leading diagnostic kit manufactu-rers utilize KPL reagents in a variety ofcommercially successful diagnostickits for AIDS, tropical diseases, foodtesting and cancer. KPL's large-capaci-ty production facility is FDA-auditedwith a comprehensive documentationsystem that assures adherence to thehighest quality standards. KPL holdsproprietary technologies and patentsfor immunoassay reagents that areused for visualization and detection.

Key Products and Services:

• Affinity Purified Antibodies andConjugates

• Substrates for ELISA and Blotting • Protein Detection and Purification

Kits and Reagents • Nucleic Acid Labeling and Detection

Kits • Kits and Reagents for Detection in

Cells and Tissues • Assay Support Reagents and

Accessories • Custom and Bulk Services

New productsCat# Description Price CHF

54-11-50 Protein Detector TMB Western Blotting Kit 525.-54-12-50 Protein Detector LumiGLO Western Blotting Kit 577.-55-11-50 Protein Detector BCIP/NBT Western Blotting Kit 525.-54-30-00 Detector HRP Chemiluminescent Blotting Kit 422.1054-31-00 Express Detector Nickel-HRP, 200 µg 336.-54-31-01 Express Detector Nickel-HRP, 50 µg 115.5054-31-02 ExpressDetector Nickel-HRP, Chemiluminescent Blotting Kit 501.9055-81-10 Protein Detector AP Microwell Kit, Anti-Human 283.5055-81-50 Protein Detector AP Microwell Kit, Anti-Mouse and Anti-Rabbit 283.5060-01-00 Detector Random Primer DNA Biotinylation Kit 562.8060-01-01 Detector PCR DNA Biotinylation Kit 441.-60-01-02 Detector RNA In Vitro Transcription Biotinylation Kit 840.-60-06-00 cDNA Integrity Kit 441.-600-0008 GeneRuler Biotinylated DNA Ladder 123.9053-00-01 SureBlue Reserve TMB 1-Component Microwell Peroxidase Substrate, 100 ml 79.8053-00-02 SureBlue Reserve TMB 1-Component Microwell Peroxidase Substrate, 400 ml 231.-53-00-03 SureBlue Reserve TMB 1-Component Microwell Peroxidase Substrate,1 l 357.-54-71-01 LumiGLO Reserve Chemiluminescent Substrate Kit 703.5054-71-00 LumiGLO Reserve Chemiluminescent Substrate Kit 207.9054-13-50 Protein Detector LumiGLO Reserve Western Blotting Kit 892.50

Mission StatementTo provide innovative protein detection technologies, reagents, andsystems with expert support services to life science researchers andcommercial partners worldwide.

You are cordially invited to tryKPL's new SureBlue ReserveTMsubstrate.

Learn more about nucleic acid labe-ling and detection and. SAVE on yournext purchase of DetectorTM Kits.

NEW LumiGLO ReserveTM Substratesand Western Blotting Kit provide thehighest sensitivity and lowest bak-kground in Western Blotting.

www.kpl.com


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Cat# Description Seize Price CHFBaseplate786-185 PROTEIN- FoldaseTM Kit 1kit 636.-786-186 FoldaseTM-I 100ml 367.786-187 FoldaseTM-II 100ml 226.786-188 FoldaseTM-III 100ml 442.-786-189 FoldaseTM-IV 5x1.5ml 211.-

A Kit For the Optimization of ProteinPurfication Strartegy

Purification of a novel protein is often adifficult undertaking. If a researchlaboratory is not sufficiently experien-ced in dealing with the purification ofnovel proteins then the task of purify-ing a protein could become a very fru-strating undertaking. Considerabletime and money may simply be wastedin testing various purification strate-gies.

The Protein Master- STRATEGYTM is akit designed to simplify the protein

purification and optimization procedu-res. The STRATEGYTM uses carefullyplanned protein extraction buffers,methods of protein estimation, andstep-by-step purification protocols.When carefully followed, protein purifi-cation can be optimized within a fewdays.

The kit guides the researcher step-by-step through various protein purifica-tion techniques, which include variousprotein precipitation-fractionation andchromatography techniques. TheProtein Master- STRATEGYTM is supp-lied with agents needed to test various

purification techniques. No expensiveequipment is needed.

After optimizing the purification strate-gy, the agents and supplies used in thekit may be ordered separately. In addi-tion, custom made columns and buf-fers are also available for further puri-fication works.

The PROTEIN-FoldaseTM kit is designedto simplify the optimization of proteinfolding procedures. This kit is based ona proprietary combination of denatu-ring agents for solubilization of inclu-sion bodies and the folding protocolbased on rapid dilution of denaturedprotein into proprietary folding mixes(e.g., FoldaseTM). Given the fact thatevery protein has unique folding pro-perties, the kit offers a selection of fol-ding mixes. These folding mixes inclu-

de FoldaseTM-I, FoldaseTM-II, FoldaseTM-III & FoldaseTM-IV. These folding mixesare based on successfully used foldingagents Polyethylene Glycol,Cyclodextrine, ND SB-201 detergent,and Ox-Re agents ( oxidizing-reducingproprietary agents), respectively. Thesefolding agents may be used individuallyor mixed with each other to createvarious combinations of the foldingagents. PROTEIN-FoldaseTM kit offers asimple to follow protocol which allows

users the option to quickly optimize fol-ding protocol for their protein.

The kit is supplied with all of the agentsneeded for optimizing protocol andreaction cups. After optimization of fol-ding protocol, any FoldaseTM reagentmay be re-ordered in larger quantitiesfor preparative scale works.

Protein Purification and Optimization SystemsProtein Mastersm- STRATEGYTM

Optimization of Protein Folding ProceduresPROTEIN- FoldaseTM

Cat# Description Seize Price CHFBaseplate786-184 Protein Mastersm- STRATEGYTM 1 kit 580.-

www.genotech.com




Life sciences

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BioWest was founded in 1987 in theheart of the Loire Valley, France. Sincethen we have evolved into one of thefinest and leading European producersof sera, media and biochemicals forcell culture.

For more than 10 years BioWest wasthe producer of top quality sera forBoehringer Mannheim (now RocheDiagnostics).When Roche decided to end theirdistribution of cell culture sera in 1999,they handed this business over toBioWest.

Products range

1. Animal Sera : FBS ( > 46 different qualities (1) )Other animal sera ( Bull, Calf, Cat, Chicken, Dog, Donkey, Horse,Foal, Goat, Guinea Pig, Lamb, Mouse, Pig, Rabbit Rat, Sheep )

2. Synth. Culture Media3. Antibiotics4. Antibodies5. Antisera & Conjugates6. Chemokines7. GF & Cytokines8. Neutrophines9. Salts & salt solutions10. Human Products11. Vitamins12. Others

This step marked the beginning of anew era – since then we have been pro-moting our own brand,“BioWest”,directly to end users and through part-nerships with specialist distributioncompanies all over the world.

Animal Sera - Fetal Bovine SerumUnity Quantity Price CHF

S1500 Fetal Bovine Serum (Canada Origin) 100 ml 10 to 49 64.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Canada FDA approved 100 ml 1 to 9 71.-

500 ml 10+ 341.-500 ml 1 to 9 370.-

S150D Fetal Bovine Serum (Canada Origin), Dialysed with 10 000 Dalton 100 ml 85.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Canada FDA approved 500 ml 313.-S150F Fetal Bovine Serum (Origin Canada) Charcoal stripped 100 ml 114.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Canada FDA approved S150G Fetal Bovine Serum (Canada Origin), Gamma radiated 100 ml 62.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Canada FDA approved 500 ml 212.-S150L Fetal Bovine Serum,Origin Canada,Lipid free 100 ml 123.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Canada FDA approved

BioWestSolutions for your Cell Culture

(1) Origins Offered:European Sera / EC approved (the only sera test-ed for BSE after the EU regulations 98/272/EC)France DanishUSDA / EC approved SeraGeographical Bovine Sp. En. risk classification(GBR)Argentinia (GBR I*)Australia (GBR I*)Brazil (GBR I*)Chile (GBR I*)Nicaragua (GBR I*)Paraguay (GBR I*)Uruguay (GBR I*)* GBR = Geographical Bovine SpongiformEncephalopathy Risk ClassificationCategory I: Highly unlikely to present a BSE riskCategory II: Unlikely, but a BSE risk cannot beexcluded

www.biowest.net


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Animal Sera - Fetal Bovine SerumUnity Quantity Price CHF

S152I Fetal Bovine Serum (USA Origin),IgG free 100 ml 142.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: US FDA approved 500 ml 474.-S1550 Fetal Bovine Serum (Uruguay ) 100 ml 10+ 39.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Uruguay FDA approved 100 ml 1 to 9 47.-

500 ml 10+ 142.-500 ml 1 to 9 173.-

S155H Fetal Bovine Serum (Uruguay), Heat inactivated 100 ml 52.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Uruguay FDA approved 500 ml 177.-S1600 Fetal Bovine Serum (Central America) 100 ml 100+ 66.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: US FDA approved 100 ml 10 to 99 71.-

100 ml 1 to 9 76.-500 ml 10 to 99 360.-500 ml 1 to 9 379.-

S1700 Fetal Bovine Serum (Australia Origin) 100 ml 10+ 57.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Australia FDA approv 100 ml 1 to 9 66.-

500 ml 10+ 370.-500 ml 1 to 9 417.-

S1750 Fetal Bovine Serum (Danish Origin) 100 ml 41.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Denmark FDA approved 500 ml 133.-S1800 Fetal Bovine Serum 100 ml 10+ 30.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: EC approved 100 ml 1 to 9 38.-

500 ml 10+ 114.-500 ml 1 to 9 123.-

S180C Fetal Bovine Serum, Calcium stripped 100 ml 66.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest S180F Fetal Bovine Serum, Charcoal stripped, Hormon free 100 ml 133.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest S180I Fetal Bovine Serum, Gamma Globulin free(< 5mg/liter IgG + IGM) 100 ml 83.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: EC approved 500 ml 256.-S180L Fetal Bovine Serum, Lipid free 100 ml 83.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest S180R Fetal Bovine Serum ,Iron supplemented 100 ml 100+ 24.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest 100 ml 10 to 100 27.-

100 ml 1 to 9 30.-500 ml 100+ 123.-500 ml 10 to 100 133.-500 ml 1 to 9 171.-

S180S Fetal Bovine Serum, Stem cell tested 100 ml 47.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest 500 ml 180.-S180V Fetal Bovine Serum, BVD free 500 ml 155.-State: Liquid Storage: - 20 ∞C Life: 48months S180X Fetal Bovine Serum, Double Charcoal Stripped, Hormon Free 100 ml 161.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest S1810 Fetal Bovine Serum (South America) 100 ml 10+ 28.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest 100 ml 1 to 9 38.-

500 ml 10+ 114.-500 ml 1 to 9 123.-

S1820 Fetal Bovine Serum (French Origin) BSE tested 100 ml 10+ 32.State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest 100 ml 1 to 9 41.-

500 ml 10+ 123.-500 ml 1 to 9 133.-

S1850 Fetal Bovine Serum, EU approved 100 ml 24.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest 500 ml 104.-S185H Fetal Bovine Serum, EU approved, Heat inactivated 100 ml 27.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: BioWest 500 ml 108.-S1870 Fetal Bovine Serum (Immuno Quality) 100 ml 17.-State: Frozen Storage: - 20 ∞C Life: 48months Origin: Mixed Origin 500 ml 76.-




Life sciences

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TMB (3,3´, 5, 5´- tetramethylbenzidine)is a substrate for horseradish peroxi-dase (HRP). Our mono-component, liq-uid TMB solutions combine TMB, sub-strate buffer and hydrogen peroxide fora safe, ready-to-use, one step, chro-mogen system. The products do notcontain harmful solvents.Recommended for ELISA procedures,liquid TMB yields a soluble blue endproduct read at 655 nm. The TMB reac-tion may be stopped with 0.2 M H2SO4resulting in a yellow color read at 450nm. Stability life time is more than 2years at 4°C.

The TMB PLUS has a higher activitylevel, resulting in a higher sensitivityand a reduced incubation time thanfound using the TMB ONE. TMB is asubstrate for HRP. The mono-compo-nent, liquid TMB solution combineTMB, substrate buffer and hydrogenperoxide for a safe, ready-to-use, onestep, chromogen system. The productsdo not contain harmful solvents.Recommended for ELISA procedures,liquid TMB yields a soluble blue endproduct read at 655 nm. The TMB reac-tion may be stopped with 0.2 M H2SO4resulting in a yellow color read at 450nm. Stability life time is more than 2years at 4°C.

The TMB Blotting/TMB Blotting PLUSare ready-to-use substrates containing3,3´, 5, 5´ tetramethylbenzidine andhydrogen peroxide. These substratesare intended for use in blotting proce-

dures with peroxidase labelled anti-bodies. The positive reaction gives astable blue colour, and the productsgive a good sensitivity and a low back-ground staining. Stability life time is 21/2 years at 4°C.

OPD, Ortho-Phenylenediamine, is usedas a chromogen substrate in ELISAprocedures with an absorption maxi-mum at 492 nm and produces a yellowcolour during the enzymatic degrada-tion of hydrogen peroxide by horserad-ish peroxidase. After addition of sulfu-ric acid you will obtain a very stable endsolution. The fizzing, buffer containingtablets are dissolved in distilled waterwithin few minutes. You get the maxi-mum convenience,- an easy, safe andreliable way to operate. Stability lifetime is 18 months at 4°C.

AEC (3-amino-9-ethylcarbazole) isused as a chromogen substrate inimmunoblotting and immunohisto-chemistry. AEC produces a rose-redcolour during the enzymatic degrada-tion of H2SO4 by horseradish peroxi-dase. Each tablet contains 5 mg of AECand dissolves in approx. 5 minutes. 1tablet is added 1 ml of 96 % alcohol,followed by addition of distilled water to10 ml.This will give a solution of pH 5.1+ 0.1 buffered with 20 mM citrate.Stability life time is 18 months at 4°C.

DAB (3,3-diaminobenzidine tetrahy-drochloride), is used as a chromogensubstrate in immunohistochemistry.

DAB produces a brown colour duringthe enzymatic degradation of H2SO4 byhorseradish peroxidase. Each tabletcontains 10 mg of DAB and dissolves indistilled water in less than 5 minutes.Stability life time is 18 months at 4°C.

Fast Red is used as a chromogen sub-strate in immunoblotting and immuno-histochemistry using alkaline phos-phatase antibody conjugates. Eachtablet contains 0.5 mg naphthol phos-phate, 2 mg FAST RED chromogen and0.4 mg levamisole, an inhibitor ofendogenous enzyme activity. Stabilitylife time is 18 months at 4°C.

The Urea Peroxide tablets is the safeand easy alternative for the addition ofhydrogen peroxide. One tablet dis-solved in 10 ml of the substrate solu-tion will give a concentration of 6 mMH2O2. Stability life time is 18 months at4°C.

Avidin is purified from hen's egg whiteby affinity chromatography. The purifi-cation procedure ensures minimal con-tamination by other proteins or DNA.The resulting high activity and purity ofthe product gives very low non-specificbinding.

The HRP is purified by repeated affinitychromatography of root extracts fromhorseradish. The result is an enzyme ofhigh specific activity. Our horseradishperoxidase is purified in large scale bythe use of benzhydroxamic acid

Immuno reagents from Denmark (DK)

Kem-En-Tec is a biotechnology company founded in 1988 by a team ofDanish scientists from the Protein Laboratory at the University ofCopenhagen.

The products are typically used in hospitals, at universities, private andindustrial research laboratories, and for various industrial purposes.

Range of biotechnological products:

Immunological reagents as Avidin, Streptavidin, Alkaline Phosphatase,Horseradish Peroxidase and Enzyme Substrates. The Enzyme Substratesinclude tablets (OPD, AEC, DAB, Fast Red) and several versions of Mono-component Liquid TMB.

www.kem-en-tec.com


11

agarose giving a product of high purityand specific activity. The product con-sists mainly of the basic isoenzymesand are supplied as lyophilized powder.Activity: >900 U/mg (25°C, ABTS andH2O2 as substrates) .

The Steptavidin is produced by fermen-tation of S. avidinii in a minimal medi-um. The protein has a structure withfour identical subunits, each containinga binding site for biotin. It is stable overa wide range of pH and decomposesonly in the presence of SDS at temper-atures above 60°C. Steptavidin conju-gated with an enzyme, i.e. HorseradishPeroxidase or Alkaline Phosphatase ora fluorochrome may be used to visual-ize biotin labelled antibodies.Compared to Avidin, Steptavidin has alower non-specific binding.

The Alkaline Phosphatase is purifiedfrom calf intestine by a method devel-

oped in our laboratories, involvingaffinity chromatography. This enzymeis widely used as a marker enzyme inapplications such as:

•ELISAImmunoblot t ingImmuno-histochemistry Molecular BiologyActivity: >630 U*/mg enzyme protein(25°C, 4-nitrophenyl phosphate, glycineas buffer)• Unit definition: That amount ofenzyme causing the hydrolysis of onemicromole of p-nitrophenyl phosphateper minute at pH 9,6 and 25°C.

The pNPP (p-NitroPhenyl Phosphate)liquid, ready-to-use substrate is ahighly active and stable formulationutilized for measuring AP- (AlkalinePhosphatase) probe activity. The solu-tion is a solvent free buffer solutioncontaining stabilized pNPP using sodi-um azide as preservative. In kineticassays p-nitrophenol appears yellow

and is measured at 405 /650 nm. Ifstopped reactions are preferred, addequal amounts of 2 .0 M sodiumhydroxide solution and read theabsorbance at 405 /650 nm.

The liquid BCIP/NBT is the most com-monly used substrate for colorimetricdetermination of Alkaline Phosphatase(AP) activity in membrane tests. Weoffer an aqueous buffered solution con-taining NBT (NitroBlue Tetrazolium)and BCIP (Bromo-Chloro-IndolylPhosphate) forming a blue/purple pre-cipitate at the positive sites. The colourdevelops when AP catalyses thedephosphorylation of BCIP. The intenseblue/purple precipitate is very stablealso resisting fading when exposed tolight.

Price list for some typical products

Reference Product Quantity Price CHF

4090 OPD Tablets, 2 mg 1 x 250 pcs. 124.-4170 DAB Tablets 10 mg pH 7 1 x 50 pcs. 124.-4190 AEC Tablets 5 mg pH 5.1 1 x 50 pcs. 98.204210 FAST RED Tablets (2mg) 1 x 50 pcs. 98.204260 OPD Tablets 10 mg 1 x 100 pcs. 152.504360 Urea Peroxide Tablets, 5.68 mg 1 x 100 pcs. 98.204020A Avidin 1000 mg 1'395.504020C Avidin 10 mg 102.104030B Avidin, HRP-conjugated 264.904060B Protein A, HRP-conjugated 348.904120A Horseradish peroxidase 1000 mg 894.204120E Horseradish peroxidase 25 mg 98.204380A TMB ONE 1 x 1000 ml 387.604390A TMB PLUS 1 x 1000 ml 465.204390H TMB PLUS 1 x 100 ml 107.304400A p-NPP Substrate 1 x 1000 ml 568.504400H p-NPP Substrate 1 x 100 ml 90.504410A BCIP/NBT Substrate 1 x 1000 ml 568.504410H BCIP/NBT Substrate 1 x 100 ml 90.504420A BCIP/TNBT Substrate 1 x 1000 ml 682.204430A Prestained TMB ONE 1 x 1000 ml 413.504430H Prestained TMB ONE 1 x 100 ml 102.104440A Prestained TMB PLUS 1 x 1000 ml 491.-4440H Prestained TMB PLUS 1 x 100 ml 118.904450A TMB Blotting 1 x 1000 ml 387.604460A TMB Blotting Plus 1 x 1000 ml 465.204520A Blocking buffer 1 x 1000 ml 192.504530A HRP Stabilizer 1 x 1000 ml 348.904540A AP stabilizers 1 x 1000 ml 348.904611A Streptavidin, salt free 1000 mg 3'566.204611C Streptavidin, salt free 10 mg 514.304700A Alkaline Phosphatase 1000 mg 9'820.-4700C Alkaline Phosphatase 10 mg 387.70




Life sciences

12

Molecular Biosciences is a major,domestic manufacturer of productsused for life science research.Presently, the company produces over500 high purity research tools andreagents such as: water solublebiotinylation reagents, vitamin deriva-tives, crosslinking reagents, lipophilicprobes, fluorophores, radioiodinationreagents, and dendritic cores forpreparing oligomers. These materialsare used to modify polypeptides,oligonucleotides, monoclonal antibod-ies as well as other biomolecules andsurfaces. The products are used pri-marily to investigate mechanisms of

cellular function for maintaininghomeostasis. The company accentu-ates new products and developingtechnology for diagnostics, drug deliv-ery and pharmaceutical research.

New Reagents:

1. Photoreactive & Water Soluble Biotinylation Reagent !!!#00491; (+)-Biotin-PEO4-ANB

3. Excellent, High Quality, Commonly Used Laboratory Reagents atExceptional Prices !!!Examples: NHS, DCC, DMT-Cl, CDI, BOC-anhydride, HOBt (anhydrous), TCEP, 4-DMAP

This biotinylation reagent incorporates anANB-PEO4 tether. The extra long 14-atomspacer reduces steric problems when bind-ing avidin and imparts highly desirablewater solubility properties to this reagent.The microcrystalline material dissolves toprovide "water-white" solutions of labelingreagent for easy dispensing. Covalentattachment to substrate is achieved byphotolysis at 320-350 nm for 20 min.

This biotinylation reagent incorporates anANB-PEO4 tether. The extra long 14-atomspacer reduces steric problems when bind-ing avidin and imparts highly desirablewater solubility properties to this reagent.The microcrystalline material dissolves toprovide "water-white" solutions of labelingreagent for easy dispensing. Covalentattachment to substrate is achieved byphotolysis at 320-350 nm for 20 min.

All materials exceed Molbio release speci-fications. See listings under Research Bio-chemicals.

Products

• BiotinsResearch • Natural Products• Fluorescent Probes• Homobifunctional NHS esters • Multifunctional Crosslinkers• Radioiodination Reagents • Research Biochemicals & Buffers• Bioligands• Heterobifunctional Crosslinkers• Homobifunctional Maleimides • Photoactive Reagents• Research Biochemicals & Buffers

Molecular Biosciences Products that accentuate your creativity !

What's New at Molecular Biosciences?

2. Powerful Transfection Reagent !!!#88418; DC-Cholesterol (Molbio Ultra Grade, 99+%)

www.molbio.com


13

Biotinylation Reagents

Cellular Probe Reagent

Crosslinking Reagent

(+)-Biotin>99% #00671 1.0 g 58.80 (+)-Biotin-PEO4-psoralen #80142 10 mg 262.50 NHS-(+)-Biotin #00678 100 mg 94.50 (+)-Biotinynl-PEO3-fluorescein #00695 5 mg 262.50 sulfo-NHS-(+)-Biotin #00134 50 mg 136.50 (+)-Biotin-PDA #00402 100 mg 220.50 sulfo-LC-(+)-Biotin #00598 100 mg 199.50 (+)-Biotin hexylamine #00211 100 mg 224.70 C-(+)-Biotinhydrazide #00785 50 mg 115.50 LC-(+)-Biotin-PEO4-amine #00245 50 mg 157.50 SLC-NHS-(+)-Biotin #00322 100 mg 210.- (+)-Biotin-PEO6-(+)-Biotin #00707 50 mg 210 .-(+)- Biotin-PEO3-amine #00215 100 m 262.50 (+)-Biotin-PEO3-maleimide #00944 50 mg 203.70

#00391 100 mg 136.50 #00415 25mg 312.90Amine Reactive Water soluble

#00523 100 mg 115.50 #00987 50 mg 210.-Amine Reactive Water soluble

#80059 59 mg 210.- #88407 250 mg 210.-

#23001 1.0 g 76.50 #03494 100 mg 182.70

#24643 50 mg 157.50#88155 10 mg 178.50

#71977 1.0 g 126.-

#67432 100 mg 210.-

#98321 500 mg 315.- #22711 1.0 g 315.-

#19675 50 mg 199.50

#43660 50 mg 157.50

#98661 50 mg 210.-

#43911 100 mg 210.-




Life sciences

14

Seegene' s major objectives have beento provide the products of only highestquality and to have the life scientistsachieve their research goals and time-lines, resulting in significant advancesin the their research areas. We nevercompromise our high standards of thequality of our products for the sake ofthe client satisfaction. Every product isserved with a strong emphasis on effi-ciency and reliability to satisfy theneeds of researchers. Our researcherswill be consistently doing their best toprovide optimal results and products.Through a combination of our consis-tent efforts and your satisfaction, ourproducts will remain precious andvaluable in quality and performanceand continue to be standards by whichothers are measured.

Seegene Inc. is not only committed toproducing quality products for yourpresent needs but also recognizes thatthe development of more advancedtechnology is necessary for continued

success. Owing to our large portion ofdevotion toward research and develop-ment in molecular biology field, wehave recently developed a new con-cept-based technology named "ACP¢‚Technology" (patent pending).

Seegene's proprietary ACP¢‚Technology is the world's first true"Annealing Control Primer" system fordramatically improving specificity andsensitivity in Hybridization/PCR. As abreakthrough in Hybridization/PCRplatform, our technology has potentialto fundamentally alter the principles ofthe current existing Hybridization/PCRapplications and to upgrade them atone time. The surprising effect of theACP¢‚ Technology has been proven invarious fields, such as Gene Discovery,SNP genotyping analysis, MultiplexPCR, etc... To the end, we would like tomake a great contribution towardbiotechnology by having our technologyvalued by and prosperous of scientistsin the worlds.

SeegeneCompagny profile

GeneFishing™ DEG KitsAll of the GeneFishing¢‚ DEG kits (DEG101~106) comprise 20 rans-domly selected arbitrary ACPs (Annealing Control Primers) and eachDEG kit works equally for your target samples.

Full-lenght cDNAsSeegene's Full-length cDNAs are ideal to study gene expression inspecific tissues and at specific developmental stages and also toclone the genes belonging to a multigene family.

Pre-made Northern BlotsNorthern blots are pre-made for immediate use and designed to SeeGene expression in specific tissues and at specific developmentalstages. Our Northern blots allow you to assess the distribution, size,alternative splicing forms, and level of your transcripts in one exper-iment.

Zoo BlotWe are offering zoo blot(pre-made Southern blot) including 12 differ-ent species for your screening assays. Genomic DNAs were preparedfrom human, rat(SD), mouse(ICR), dog, cow, pig, rabbit, chicken,frog(Xenops), fish(Zebra fish), C. elegans, and yeast.

Genomic DNAsWe are offering genomic DNAs obtained from 12 different samplesources for your screening assays. Seegene's genomic DNA is quali-fied for genomic analysis including PCR and library construction.

100bp DNA ladderOur unique 100 bp endless usage ladder system (patent pending) isclearly distinguished from any existing commercialized consumables100 bp DNA ladder. This system supplies templates (plasmids) whichwilll be amplified to be used for size markers.

Seegene Genefishing™ Kit

First commercially available GeneFishing™

application Kit to identify only authenticDifferentially Expressed Genes (DEGs)!

• ONLY authentic DEGs delivered!• NO false positives!• NO PAGE gel!-agarose gel is sufficient • NO radioactivity!• NO specific skill required!• Long-distance PCR products rangingfrom 150 bp to 2 kb!

GeneFishing™ Technology is a new andunique approach capable of identifying onlyauthentic Differentially Expressed Genes(DEGs) in two or more nucleic acid sam-ples. This technology dramaticallyimproves specificity and sensitivity of PCRamplification so that it enables theresearchers to find only real PCR productsas a result. The GeneFishing™ DEG kit isthe first application of Seegene's propri-etary technology based on innovativedesign of the primer (Annealing ControlPrimer, ACP™, patent pending).

How it worksTypical Results [email protected] manual

Order Information • GeneFishing™ DEG Kit Components • 20 Arbitrary ACPs (each for 20 reactions)• Anchor ACP (for 200 reactions)• 5' Universal primer• 3' Universal primer• Control cDNAs: Mouse conceptus cDNAs(E4.5 and E18.5)

Shipped at ambient temperature, store at -20C

}

www.see-gene.com


Gene Fishing™ new technology to identify differentiallyexpressed genes.Seegene’s PCR based GeneFishing technology offers the following unique

advantages over conventionalmethods

NO false Positives !

Agarose Gel is sufficient - no polyacrylamide gels, no radioactivity

No expensive equipment, no DNA chips nececessary

High reliability and reproducibility

Easy to use and cost effective

Fast : results within 5 hours

Long Range PCR products are generated (up to 2 kb)

Two or more RNA samples from any organism can be compared

Premade northern blots from Mouse an rat tissues & zoo blot

Socochim now offers SeeGene’s Premade Northern Blots form mouse and rat tis-sues at different developmental stages. A zoo blot with genomic DNAs from differ-ent species is also available.

15

Genefishing™ DEG101 Price listItem Cat.# Price CHF

GeneFishingTM DEG101 DEGK 3101 1'920.-GeneFishingTM DEG102 DEGK 3102 1'920.-GeneFishingTM DEG103 DEGK 3103 1'920.-GeneFishingTM DEG104 DEGK 3104 1'920.-GeneFishingTM DEG105 DEGK 3105 1'920.-GeneFishingTM DEG106 DEGK 3106 1'920.-

Premade northern and zoo blot price listItem lane Cat.# Price CHF

GMouse Conceptus Stage Blot 15 MCSB 1001-1 820.-Mouse Embryo Stage Blot 15 MESB 1002-1 820.-Mouse Placenta Stage Blot 9 MPSB 1003-1 740.-Mouse Uterus Stage Blot 13 MUSB 1004-1 740.-Mouse Conceptus Tissue Blot 8 MCTB 1005-1 660.-Rat Conceptus Tissue Blot 8 RCTB 1005-2 660.-Mouse Adult Tissue Blot 13 MATB 1006-1 820.-Rat Adult Tissue Blot 14 RATB 1006-2 820.-Rat Brain Tissue Blot 9 RBTB 1008-1 820.-Mouse Brain Aging Blot 10 MBAB 1009-1 1'680.-Rat Brain Aging Blot 10 RBAB 1009-2 1'680.-Mouse Mammary Aging Blot 11 MMAB 1010-1 1'680.-Zoo Blot 12 ZOOB 2001-1 900.-

cDNA Product LinesFull Lenght cDNAs fromMouse, Rat, Rabitt andChickenSocochim is pleased to annouceSeeGene’s premade cDNAs for study-ing expression in specific tissues and atspecific develomental stages and alsoto clone the genes belnging to a multi-gene family. Primers to perform RACEare supplied with th cDNAs.

[email protected] for further information




Life sciences

16

FASTsilverTM

For Protein and Nucleic Acids

A unique formulation of reagents thatleaves the background clear and pro-duces sharp images of protein andnucleic acid bands. FASTsilverTM

detects up to 1ng protein.

The kit contains ready to use reagentsfor 25 mini gels and comes with a sim-ple to follow 60-90 minutes protocol.

PN-SilverTM D-StainStain, de-stain, & re-stain your proteinor nuclic acid gels.

If your silver stained gels are too darkfor analysis or if you do not want to runthe same sample twice use PN-SilverTM

D-Stain . It will allow you to reducebackground staining and reveal bandsnot discernable otherwise. Or de-stainthe gels completely and re-stain yourgels or follow other downstream appli-cations with your gels, such as Westerntransfer, sequencing etc. Suitable forboth protein and nucleic acid gels.

Glyco-StainTM

For staining of glycoprotein after elec-trophoresis. A modified silver stainbased method allows glycoprotein sen-sitivity at non-gram level ( ~1ng/band).

RAPIDstainTM

DESTAINING NOT REQUIRED

Is 5-10 Minutes Rapid Enough ForStaining Protein Gels?

1. Truly Rapid

An ultrasensitive and ready-to-useprotein stain based on Coomassie dye.No mixing or preparation is involved.No fixation step is needed. Simply pourRAPIDstainTM on your gel, and bandsdevelop before your eyes within 5-10minutes and reaches a maximum in 60minutes. You can monitor the bands

developing directly in the staining tray.Mini-gels can be stained in 10 minutes.

2. Destaining Not Required

RAPIDstainTM only stains protein leav-ing a crystal-clear background result-ing in a high band visibility. The gelsrequire no destaining. The proteinband intensity is enhanced by simplyrinsing with water.

3. Sensitive

RAPIDstainTM has the sensitivity todetect as little as 4-8ng BSA. Stainingshows a linear response for densito-metric gel analysis and produces sharpscanning or photographic results.

OTHER ADVANTAGES OF RAPID stainTM

•Saves time, save expenditure andreduces waste

•Uses a single step method of visualiz-ing protein bands in gels

•Avoids the use of methanol, aceticacid, or other toxic agents

• Works with SDS-PAGE or native PAGE

•RAPIDstainTM is supplied in a1x1000ml, sufficient reagent to stainup to 50 mini gels (8x10cm) or 30-40larger gels.

•RAPIDstainTM is also available in gal-lon (3,785 Liter) size for staining up to200 mini gels.

5 Minute Reversible Copper StainTM

The 5 Minute Reversible Copper StainTM

is a single step stain for the rapiddetection of proteins fractionated bySDS-PAGE. No de-staining is neces-sary. The stain is based on the interac-tion of copper ions with polyacrylamideand proteins. The stain works bydepositing a copper metal precipitatein the gel which turns the gel opaquegreen, while the SDS coating on theproteins prevents the stains from bind-ing to the proteins. A negative image is

produced; clear protein bands aredetected against a semi-opaque bluepolyacrylamide background. Proteinbands are visualized in as little as 5minutes. The sensitivity of the 5 MinuteReversible StainTM is 8-12ng . Stainingdoes not interfere with the electroelu-tion of proteins or alter their biologicalproperties. Gels stained with the 5Minute Reversible Copper StainTM canbe de-stained in 20-25 minutes beforethe transfer or electroelution of pro-teins.

The kit contains ready to use reagentsfor 25 mini gels and supplied withdestainer.

5 Minute ReversibleZinc StainTM

The 5 Minute Reversible Zinc StainTM isa single step stain for the rapid detec-tion of proteinsfractionated by PAGE(native gels or SDS denatured gels). Node-staining is necessary. The stain isbased on the interaction of Zinc ionswith polyacrylamide and proteins. Thestain works by depositing a zinc metalprecipitate in the gel which turns thegel opaque white, while the SDS coat-ing on the proteins prevents the stainsfrom binding to the proteins. A negativeimage is produced; clear protein bandsare detected against a semi-opaquewhite polyacrylamide background.Protein bands are visualized in as littleas 10-15 minutes. The sensitivity of the5 Minute Reversible Zinc StainTM is 8-12ng and does not interfere with theelectroelution of proteins or alter theirbiological properties. Gels stained withthe 5 Minute Reversible Zinc StainTMcan be de-stained in 5 minutes beforethe transfer or electroelution of pro-teins.

This stain works with native as well asSDS denatured gels and gels contain-ing Glycine, Tricine and a variety of pri-mary-amine containing buffers.

ELECTROPHORESIS – Gel StainsA selection of kits for staining protein and nucleic acidsfrom Geno Technology, Inc.

www.genotech.com


17

FOCUS-FastSilverTM

Use MassSpec Compatible SilverStaining of Proteins Gels for EnhancedPerformance

A sub-nanogram sensitive silver stain-ing kit FOCUS-FastSilverTM producescrystal clear background and maximalpeptide recovery needed for criticalanalysis by mass spectrometry.

For mass spectrometry analysis youneed complete digestion and recoveryof peptide. Residual silver ions used intraditional kits inhibit trypsin digestion.Glutaraldehyde used as sensitizer inmany silver staining methods modifieslysine residues preventing completedigestion and recovery of peptides.

FOCUS-FastSilverTM does not use pro-tein modifier glutaraldehyde and thekit is provided with silver-ion removingand de-staining reagent SilverOUTTM -which allows complete trypsin diges-tion and extraction of peptide neededfor mass spectrophotometry.

Features of FOCUS-FastSilverTM

Full & Efficient Trypsin Digestion.

Silver has been found to inhibit trypsindigestion by binding at the active site oftrypsin and chymotrypsin.

FOCUS-FastSilverTM is provided withSilverOUTTM reagent for removal of sil-ver ions from protein bands in poly-acrylamide gels. This allows maximaltrypsin digestion.

Full and Efficient Recovery ofPeptides. FOCUS-FastSilverTM does not use glu-taraldehyde used in traditional silverstaining protocols. Glutaraldehydemodifies lysine residues and cross-linkproteins, reducing peptide recovery.

FOCUS-FastSilverTM allows enhancedtrypsin digestion and efficient recoveryof digested peptides.

Sensitivity ~ 0.3ng protein and crystalclear background for maximum sensi-tivity.

Short protocol time- protein can bedetected in less than 90 minutes.

SilverOUTTM - for those who prefer touse their own silver staining methodwe offer SilverOUTTM reagent forremoval of silver ions before trypsindigestion.

Ordering informationCat.# Description Seize Price CHF

786-30 FAST silver TM 25 Mini-gels 277.50786-199 PN-Silver TM D-Stain 25 Mini-gels 213.50786-254 Glyco-StainTM 25 Mini-gels 377.50786-31 RAPIDstainTM 1x 1000ml (50 mini gels) 126.50786-31G RAPIDstainTM 1x gallon (200 mini gels) 389.-786-32 5 Minutes Reversible Copper

Stain & DestainerTM 25 Mini-gels 222.-786-32DS 5 Minutes Reversible Stain

DestainerTM 500ml 222.-786-32ZN 5 Minutes Reversible Zinc

Stain & DestainerTM 25 Mini-gels 222.-786-32DS 5 Minutes Reversible Stain

DestainerTM 500ml 222.-786-240 FOCUS- FastSilverTM 25 gels 344.50786-244 SilverOUTTM 100 Preps 166.50

How To Select a Protein Gel Stain - A Selection Guide

The comparative study on the following protein gel stains wwas performed using BSA as a protein standard and 4-20% SDS-PAGE gel containing 10-15 wells. The sensitivity is defined as the amount of BSA in the faintest band. The sensitivity, ng/mm2,refers to the cross section of the sample well.

Stain

RAPIDStainTM

5 Minute Reversible Copper StainTM

5 Minute Reversible Zinc StainTM

FastSilverTM

FOCUSFastSilver

Glyco-StainTM

Sensitivityng/band

8-10

8-12

8-12

0.5-1.0

0.5-1.0

0.5-1.0

Sensitivityng/mm2

4-6

3-4

3-4

0.3-0.5

0.3-.05

0.3-0.5

Comments

Rapid Coomassie blue dye based stain. Simply pour stainon the gel and bands develop before your eyes. Bands arevisible within 5-10 minutes and reach maximum in 60minutes. De-staining is not required for visualizing proteinbands, however, washing with water enhances weakly stai-ned protein bands.

Reversible, simple and fast stain. Allows subsequent elu-tion, blotting, or other types of staining. Not suitable fornative gels or gels containing Tricine or Glycine

Reversible, simple, fast, and sensitive. High contrast bands.Excellent for subsequent protein elution, blotting, sequen-cing, mass spectroscopy, etc. Works with native and SDSdenatured gels and gels containing Glycine and Tricine.

Highly sensitive. Stains both proteins and nucleic acids.

Highly sensitive stain for protein. Suitalbe for MassSpec

A modified silver stain based method allows sensitivity atnon-gram leve

Staining Time(Minutes)

10-60

5-10

10-20

60-90

60-90

~3h




Price list for some typical products

Quantity Catalogue N° Price CHF New Price CHF

1000 ml AOD-1000 63.- 50.40 .-

5000 ml AOD-5000 284.- 227.20 .-

Moycoplasma off ®

Solutions for your Cell Culture

Mycoplasma-Off® is a new solution forcleansing and disinfection of all labora-tory surfaces and apparatus, includingclean benches, incubators, workbenches, cell storage boxes and liquidnitrogen containers from mycoplasmacontamination.

Mycoplasma-Off® is an alcohol-basedsolution of the powerful mycoplasmaelimination reagent Mynox®. It can beused as a routine contamination control

programme or for known or suspectedmycoplasma challenges.

Mycoplasma-Off® is outstandingly safein use. It possesses not only a strongmycoplasmacidal but was also found tobe effective against Staphylococcusaureus, Streptococcus faecalis,Escherichia coli, Proteus mirabilis,Pseudomonas aeruginosa, Candidaalbicans, HBV/HIV, Papova-, Adeno-,Polio- and Vaccinia viridae as well as

TBC. The effectiveness of Mycoplasma-Off® is further enhanced by its excellentdetergent properties, so that clean con-tamination-free surfaces can beachieved in a single application.

Mycoplasma-Off® is a ready-to-usesolution supplied in a refillable spraybottle.

20% discount untilNovember 30 th

• Completely Kills Mycoplasma within Seconds

• Convenient and Ready-to-Use

• Non-Corrosive and Non-Carcinagenic

• Stable

• Does not Leave any Residue

• Flexible in Use

We have the pleasure to distribute the wide range ofvaluable products of Amercian Diagnostica

Contact us for more inforamtion

New

Life sciences

18

www.minerva-biolabs.com

www.americandiagnostica.com


19



Special offer until November 30th 2003 !

Description Product# Price in CHF New Price in CHF

Reacti-Therm™ Heating Module (single-block) 18790 1’583.- 1’345.-Reacti-Therm™ Heating/Stirring Module (single-block) 18971 1’981.- 1’683.-Reacti-Therm™ III Heating Module (triple-block) 18840 3’180.- 2’703.-Reacti-Therm™ III Heating/Stirring Module (triple-block) 18940 3’790.- 3’220.-Reacti-Therm™ Thermometer, 0-200° 18915 147.- 124.-Reacti-Block™ 224.- 190.-

Chromatographywww.piercenet.com

Reacti-Therm™Dry Block SampleDerivatization Systems

Reacti-Therm™ Modules are easy-to-use dryblock heaters that provide constant tempera-ture control. They are ideal for the heating,stirring and evaporation required for samplederivatization prior to analysis by GC or highperformance liquid chromatography (HPLC).They are also ideal for other smali-scalereactions. Designed with a recessed cavitythat holds interchangeable Reacti-Block™Alumirium Blocks, the modules can also beused for sample incubation and evaporation,and vacuum hydrolysis for amino acid analy-sis. Choose from four module designs:single-block heating, single-blockheating/stirring, triple-block heating, and tri-ple-block heating/stirring.

- Deliver uniform dry heat with unmat-ched convenience and versatility

- Heating/stirring modules feature varia-ble speed stirring, which is ideal for promoting smooth, uniform reactions and for dissoiving residues

- Eliminate sample contamination that sometimes occurs with water baths

- Reacti-Block™ Aluminum Blocks are available for use with a wide selection ofReacti-Vial™ Smali ReactionViais, test tubes, vacuum hydrolysis tubes and general sample vials and containers


Labware

20

ApplicationOrion II is the perfect choice for allluminescent flash and glow applica-tions performed in microplate format:

• Reporter gene assays includingdual-reporter assays

• ATP assays • Kinase activity assays • Cell proliferation, cytotoxicity and

biomass assays, apoptosis assays • Quantification of nucleic acids • Ca 2+ measurements and Aequorin

based assays • Luminescence immunoassays (LIA) • Cellular luminescence • Environmental toxicity and

mutagenicity assays

The Orion II Microplate Luminometerhas been evaluated by the ResearchDepartment staff at PromegaCorporation and has been certified foruse with their Dual-Luciferase®Reporter Assay System.

Technical dataSample format: 96-well microplate, opaque in solid or strip format, configuration for both 96- well and

384-well microplate available Detector: Photomultiplier tube with bialkali cathode, effective spectral sensitivity

range 300 - 600 nm, (extended range on request), operated in photon counting mode Sensitivity: 10 attomole ATP Crosstalk: Less than 3 x 10-5 Dynamic range: 6 decades Measuring Time: 0.1 s - 100 s per well Scan Pattern: Measurement by row or by columns Random selectable single well, access by mouseclick Injectors: Up to four per Orion II system (upgradeable)two injectors in pre-position,

two in measurement position Injection Volumes: 10 - 150 µl per pump in increments of 1µlInjection Pattern: Simultaneous injection into two adjacent wells possible in column mode as well as in row mode. Tubing: Chemically inert PTFE tubing and connections (PTFE ; KEL-F; glass; PS), easily exchan-

geable liquid handling system and tipsMinimal dead volume due to short reagent lines Priming: Forward, and backward priming, built-in priming container or external priming containerShaker: Linear, orbital and cross shaking Sample Incubation: Optional sample incubation from RT+ 5°C to + 50°C , +/- 1 KAutomation: Compatible to all known microplate transfer devices Interfaces: Serial interface (RS-232) and USB interface for PC/td> Dimensions: W 385 mm, D 410 mm, H 235 mm Weight: 22kgPower: 230 V 50 Hz; 115 V 60 Hz Power Consumption: 30 VA, maximum approx. 100 VA (4 injectors and heating)Storage Temperature: 0° C - 40° COperating Temperature: 10° C - 30° C Humidity: 10% - 80% (non condensing)

Description Part N° Price CHF *New Price CHF

Orion II 96 Microplate Luminometer 11280010 31'000 .- 24'800.-Orion II 384 Microplate Luminometer, Suitable for96-well and 384-well microplates 11280020 32'100 .- 25'680.-Orion II 96 Microplate Luminometer, Sample Incubationup to 50°C 11280030 34’300 .- 27'440.-Orion II 384 Microplate Luminometer, Sample Incubationup to 50°C 11280040 35'400 .- 28'320.-

Orion II

The conceptThe Orion II microplate luminometercombines various innovative ideasresulting in the highest performancealong with configuration flexibility andeasy maintenance. The instrumentincorporates the most sophisticatedphoton counting detection technology.The combination of automatic high pre-cision reagent injectors, optional tem-perature control and the new intuitiveWindows® Software Simplicity makesthe Orion II the leading product forluminescence detection frommicroplates. The unit is available withup to four reagent injectors. Three dif-ferent modes are available for shakingof microplates. The luminometer offersboth RS-232 and USB serial ports.Loading and unloading of microplatesby robotic arms is supported.

20% save

on the followingapparatus*

www.berthold-ds.com


Description Voltage Part N° Price CHF *New Price CHF

Model FID-1000 Gas Station 220 V 97186 14'753.50 .- 11’802.80

* If ordered before Novembre 30th 2003.

21

Model FID-1000 FID Gas StationAn Alltech Exclusive!

• Generate UHP Zero Grade Air fromcompressed air

• Generate pure hydrogen (99.9995%)from deionized water

• Ideal for 1-2 FIDs• Save bench space by using one unit

to replace two generators

Gas Generator Benefits

The FID-1000 is a complete systemwith state-of-the-art, highly reliablecomponents engineered for easyinstallation, operation, and long termperformance. The Parker Balston FID-1000 eliminates all the inconveniencesand cost of zero air and hydrogen cylin-der gas supplies and dependence onoutside vendors. Incontrollable priceincreases, contract negotiations, longterm commitments, and tank rentalsare no longer a concern. With the FIDGas Generator, you control your gassupply.

Proven Technology

Parker Balston’s FID Gas Station provi-des both hydrogen gas and zero gradeair for FID detectors on GasChromatographs. This system is speci-fically designed to provide fuel gas to 1-2 Fame Ionization Detectors.

Baseline Comparison

The chromatograms compare baseli-nes produced by a Parker Balston®Zero Air Generator and bottled fuel air.The baseline produced by the ParkerBalston® Generator is very flat, withno fluctuations or peaks, in compari-son with the chromatogram of the bott-led air fuel supply, which has manypeaks ranging from 0.25ppm to -0.25ppm.

Principal Specification

FID-1000 Hydrogen Specification Zero Air SpecificationProduct Purity: 99.9995% Pure H2 <0.1ppm THCFlow Rate: 90 cc/min 1,000 cc/min Delivery Pressure: 0-90 psig 40-125 psig Inlet Connection: N/A 1/4" NPT (female) Power Requirements: 120 VAC/amp Dimensions: 16.5"H x 10.5"W x 17"D (42cm x 27cm x 43cm)Weigth Dry: 46lbs (21kg)Outlet: 1/8" Compression 1/8" Compression

www.alltechweb.com




Labware

22

Mini-fugesCentrifuges

Catalog N° Description Packaging Price CHF

J1-1653 Mini Fuge, Comes with interchangeable rotorsfor either single or strip tubes 600.-

New price550.- CHF

RBS Chemical ProductsCleaning agents / Degreasers / Disinfectants

You have cleaning needs ?Contact us, we have the solution !

Price list Product Packing Code Unit size Price in CHF New Price in CHFRBS 35 35 E032 1 L 17.20.- 13.80RBS 35 35 E024 5 L 68.- 54.40RBS 35 35 E031 30 Kg 363.- 290.40RBS 35 35 E030 220 Kg 2 387.- 1 909.60

Special offer until November 30th 2003 !

Save 20 % on the RBS 35, used as multipurpose cleaning agent (cleaning and degreasing of laboratory glassware, equipment made of platic, porcelain, ceramics, quartz, stainless steel, …)

ABgene has a wide range of other products.Consult the web site or contact us!

RBS has a wide range ofother products.Consult the web site or contact us!

www.abgene.com

www.rbs-cp.be


23



Crimp Neck ND11 with wide opening

Part N° Description Price CHF11090356 Crimp neck vial 1,5 ml, clear glass, 1st hydrol. Class 230.-11090476 Crimp neck vial 1,5 ml, clear glass, 1st hydrol. Class, label 250.-11090477 Crimp neck vial 1,5 ml, amber glass, 1st hydrol. Class, label 260.-11030209 11 mm Al crimp cap, center hole with natural rubber red-orange/ 270.-

TEF transparent, 60° shore A, 1.0 mm11030247 11 mm Al crimp cap, center hole with Ultra clean silicone white/ 658.-

PTFE red, 35° shore A, 1.3 mm11030464 11 mm Al crimp cap, center hole with silicone white/ 1164.-

PTFE blue, cross-slitted, 60° shore A, 1.5 mm11030196 11 mm Al crimp cap, center hole with PTFE red/silicone white/ 782.-

PTFE red, 45° shore A, 1.0 mm

Special offer for each order of 2000 pcs (price offer until November 30th)

The quality does not needto be expensive !

The innovative TPX Sample Vial withintegrated Glass Micro-Inser

The first Block Cover with a deep-drawn PTFE-Film

Closures with a special grade ofSilicone/PTFE Septa for critical GCand Headspace Analysis

Every kind of syringe with high quality(Germany) for a low cost !

For more information, consulte : www.microsyringes.com

or ask to SOCOCHIM SA, by phone : 021 721 04 50by E-mail :[email protected]

www.la-pha-pack.com

TopSert™ UltraClean™

Sealmat™

Syringe Needles

Dosages of Liquids: Series N

Dosage of Liquids:Serie SN

Dosage of Liquids:Serie T

Dosage of Gas and Liquids: Series H

Dosage of Gas and Liquids: Series SH

Dosage of Gas and Liquids: Series G

Dosage of Gas and Liquids: Series P

Syringes with Adjustable Internal Stopper

Syringes for Headspace and Analysis

Syringes for On-Column Injection

Syringes for GC Autosamplers

HPLC-Syringes

Syringes for TLC

Syringes for Automatic Liquid Handling

Syringes for Robbins Hydra®

Syringes with PEEK-Tubing Connectors

Special syringes


Socochim SACh. du Trabandan 28CH-1006 LausannePhone +41 (0)21 721 04 50Fax +41 (0)21 721 04 [email protected]

We are pleased to take your

orders and to help you answer

your technical questions:


Our partners :

Des

ign

: ww

w.r

ugd.

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