Nitrogen Assimilating Enzyme Activities and Enzyme and Plant
Single proteins at work. Enzyme reaction kinetics E + S k1k1 k -1 ES k2k2 E 0 + PE0E0 k3k3 E.
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Single proteins at work
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21-Dec-2015 -
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Transcript of Single proteins at work. Enzyme reaction kinetics E + S k1k1 k -1 ES k2k2 E 0 + PE0E0 k3k3 E.
Molecular Time scales
secondmilli-second
micro-second
nano-second
pico-second
femto-second
atto-second
1 s10-3 s10-6 s10-9 s10-12 s10-15 s10-18 s
Fluctuations: ET model
Flavin:NADH oxidoreductase (Fre)
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k(t) = k0e−βx( t )Fluorescence decay rate:
New tools
waiting time
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τ =
probability density of τ
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f (τ ) =
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τ = τf (τ )0
∞
∫ dτ mean waiting time
for Michaelis-Menten kinetics
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1
τ=
k2[S]
[S]+KM
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Cτ (m) =Δτ (0)Δτ (m)
Δτ 2waiting time correlation function
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CI (t) =ΔI(0)ΔI(t)
ΔI2intensity correlation function