Single Cel l Clones

Dr. Amit Kumar Dutta, Ph.D

Plant Body is made of a wide range of innumerable cell types which are successfully integrated both in terms of structure & function.

Single Cell Culture is a method of growing isolated single cell aseptically on a nutrient medium under controlled condition.

Single Cell Culture

Look Back Before……….!

G Haberlandt (1902)

Isolate & Culture single cell from the leaves of flowering plants

W H Muir (1954)

Paper raft nurse technique for the culture of single cells

A C Hildebrandt (1960)

Culture single cells of a tumerous hybrids tobacco plant using the microchamber technique.

L Bergmann (1968)

Grew the single cells first using petridish plating technique.

Look Back Before……….!

Basic Principle of Single Cell Culture is the isolation of large number of intact living cells and culture them on a suitable nutrient medium for their requisite growth and development.

Principle of Single Cell Culture

Single Cells can be isolated from a variety of tissue and organ of green plant as well as from callus tissue and cell suspension. Single cells from the intact plant tissue(Leaf, Stem, Root, etc.) are isolated either mechanically or enzymatically.

Principle of Single Cell Culture

5 Important methods are widely employed for culturing single cells.

Paper raft nurse technique

Petridish Plating technique

Microchamber Technique

Nurse Callus Technique

Microdroplet Technique

Methods of Single Cell Culture

Factors Affecting Single Cell Culture

Composition of the medium for the growth of single cell culture.

Induction of division of single cells using paper raft technique.

The initial plating cell density is very critical.

Importance of Single Cell Culture

Important for the fundamental and mutation studies.

It has wide industrial application.

Single cell culture could be used successfully to obtain single cell clones.

Plants could be regenerated from the callus tissue.

Single Cell Culture are very important in relation to crop improvement programmes.

Importance of Single Cell Culture…….!

Single Cell Culture in large –scale could become a valuable technique for industrial production of such important natural compound(Commercial purpose).

Polyploidy can easily be achieved by single cell culture.

Importance of Single Cell Culture…….!

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