Single-biomolecule Circuits With

Carbon Nanotube Wiring

Brett GoldsmithCollins LabDepartment of Physics and Astronomy

John Coroneus and Greg WeissDepartment of Molecular Biology and Biochemistry

Overview: Creating a single-biomolecule circuit

Device Preparation

Electrically Measured Reactions

First: Redox Reactions

Second: Selective Reaction

Third: Proteins

PMMA/PEG

PMMA/PEG

Source

Drain

Nanotube Devices

R = 3MW

Electrochemical Oxidation of Nanotubes

ReferenceCounter

Source Drain

PMMAcoating

Electrolytedrop

Mannik et al. PRL (2006) Goldsmith el al. Science (2007)

Oxidation Dynamics

localization: session W26

Electrochemical Reduction of Nanotubes

Production of Carboxyl Groups by KMnO4

Goldsmith el al. Science (2007)

Selective Chemistry of Nanotubes

functionalized nanotube in:

buffer

buffer with 10 mM EDC

Protein Attached Circuits: Streptavidin

Goldsmith el al. Science (2007)

Protein Attached Circuits: Lysozyme

functionalized nanotube in:

buffer

buffer with enzyme substrate

Protein Attached Circuits: Lysozyme

Enzyme withoutsubstrate

Enzyme with substrate

360

420

Summary

Engineering the Microworld at The University of California, IrvineUCI Integrated Nanosystems Research Facility

Dr. Phil Collins

Brett GoldsmithAlex KaneBucky KhalapSteve HuntDanny WanACS-PRF

Electrically Measured ReactionsFirst: Redox ReactionsSecond: Selective ReactionThird: Proteins