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Promotions for Life Scientists Issue 9 - November 2007EditorialShop!
Molecular biologyThe new generation thermal cycler reliable and reproducible thermal performance characteristics are essential elements of a good thermal cycler and the VWr Collection UnoCycler delivers every time. Each thermal block has 4 independent temperature control sensors and 8 peltier heating units that ensure accurate heating and cooling control, unsurpassed uniformity across the block surface and superb thermal cycling performance. all cyclers contain the active Sample Cooling (aSC) function that cools your sample until the heated lid reaches its target temperature. this feature reduces non-specific primer binding and extension. all instruments have a 2-year warranty.
Buy the UnoCycler at a very special price today and choose between standard and fast blocks:• ramp rate of up to 3 °Cs-1 with standard blocks• ramp rate of up to 6 °Cs-1 with fast blocks (Fast blocks give increased productivity and faster protocols)
EditorVWR International Europe bvbaHaasrode Researchpark Zone 3Geldenaaksebaan 4643001 LeuvenBelgium
CopywritingVWR International
Layout and typesettingMarketing Services VWR
PrintingStork, GermanyNo part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe.
Run76.500 copiesPublication date: November 2007Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWR Terms andConditions of Sale apply.
Issue 9, November 2007 • vALID uNTIL 31sT mArch 2008 • check ouT www.vwr.com for fuLL specIfIcATIoNs
www.vwr.com
Molecular biology• VWR Collection Unocycler and gel
documentation system• DNA extraction kits from 5 PRIME• CBS PCR workstations• BTX HT Multi-Well Electroporation • Axygen plates for Real-Time PCR and
sequencing• RNase AWAY® or DNA AWAY® Wipes• Thermo Scientific Taq Polymerase
Cell Biology• Optimise your immunoassays• Nunc OptiCell®
• VWR Collection tube rotator• Microplate readers and washers from
Thermo Scientific
Proteomics• ProteoExtract® Subcellular Proteome
Extraction Kit from Merck Biosciences• Pall Acrosep Chromatography columns• Whatman FAST® slides for protein
microarray
Lab supplies• New BD Falcon™ flip top tubes • Brand Transferpette S multichannel• Nalgene tank Liners• Spectrum Mediakap
Biosciences• Thermo Biopolymers ProjectPeptides™• BioGenes monoclonals• Biosirius Protein Investigator
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Unocycler Cat. No. Price Promo Price
Without block 732-1200P £3,563.00 N/aWith standard gradient block, 96 well 732-9986P N/a £4,367.00With fast gradient block, 96 well 732-9987P N/a £5,340.00With standard gradient block, 384 well 732-9988P N/a £4,658.00With standard gradient block, 384 well 732-9989P N/a £5,584.00Separate blocks:
96-well gradient block 732-1203P £1,575.00 £1,260.0096 well fast gradient block 732-1205P £2,720.00 £2,176.00384 well standard gradient block 732-1204P £1,861.00 £1,489.00384 well fast gradient block 732-1206P £3,007.00 £2,406.00
Buy one get one free*
It’s time for highly pure genomic DNA from any sample sourceChoose from 5 PRIME’s exciting genomic DNA purification tools to find the optimal solution ArchivePure DNA Purification System • Highly flexible and scalable liquid-based method for archive-quality dNa from a multitude of sample typesPerfectPure DNA Purification Kits • Fast and efficient column-based dNa purification from blood and cells without overloading and cloggingReady PCR DNA Purification Kits • Very fast and unique filtration method to generate PCr-ready dNa for screening
Gel electrophoresis of genomic dNa purified with the archivePure dNa Purification System
*identical kits
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Welcome to the last edition of the VWrbioMarke Shop for 2007. as preparations will shortly begin for the Christmas and New Year celebrations now is the time to give your work a treat with a choice of some excellent offers in molecular biology, cell biology and more. . .
We hope that you have an excellent finish to 2007 and enjoy our companion journal the VWrbioMarke magazine packed full of technical data and applications.
Here’s to a healthy, happy and successful 2008!
Your VWr bioMarke team
For high throughput requirements, the duo and QuattroCycler have been developed with two or four fully independent thermal bays and fully automated heated lids, please ask for details.
Special introductory offer on the UnoCycler
Great value
Contact your local VWr sales representative for full view of the benefits this new range of
instruments will provide.
ask for a demo or visit vwr.com for demo software download.
BTX HT Multi-Well Electroporation – Transfections in seconds…HT Electroporation SystemsBtX Ht systems allow you to boost productivity by optimising both electrical and biological conditions associated with electroporation, increasing yields and viability. Now entire libraries can be screened rapidly and efficiently, saving time and money.
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Model Generator Plates Plate handler Cat. No. Price Promo Price
Ht 25/200 830 ECM™ 830 6 x 25 Well EP Ht-200 732-0342P £8,560.00 £8,060.00Ht 96/200 830 ECM™ 830 2 x 96 Well EP Ht-200 732-0344P £8,560.00 £8,060.00Ht 25/100 630 ECM™ 630 6 x 25 Well EP Ht-100 732-0472P £6,110.00 £5,610.00Ht 96/100 630 ECM™ 630 2 x 96 Well EP Ht-100 732-0474P £5,930.00 £5,430.00
Save £500 on the purchase of a BTX
HT system
The BTX HT systems feature• 25 or 96 samples transfected in
just seconds• Multiple experiments in the
sample plate
Applications include• transfection of mammalian cells• transformation of bacteria and
yeast• cdNa library screening• drug screening
iN tHiS iSSUE
Genomic DNA from Product Size Cat. No. Price
Whole blood, bone marrow, packed cells and buffy coat
archivePure dNa Blood Kit 3 ml 733-1013P £18.00
Cells and fresh, frozen, fixed or paraffin-embedded tissue
archivePure dNa Cell/tissue KitarchivePure dNa tissue Kit
100 mg100 mg
733-1017P733-1021P
£18.00 £18.00
Mouse tails archivePure dNa Mouse tail Kit 100 mg 733-1024P £18.00Buccal cells archivePure dNa Buccal Kit 10 Preps 733-1027P £18.00Plant tissue archivePure dNa Plant Kit 200 mg 733-1031P £18.00Yeast & Gram-positive bacteria archivePure dNa Yeast & Gram + Starter Kit 733-1032P £18.00
PerfectPure DNA Purification Kits
Whole blood, buffy coats PerfectPure dNa Blood Kit 50 Preps 733-1068P £60.30Cultured cells PerfectPure Cultured Cell Kit 50 Preps 733-1071P £110.00
Ready PCR DNA Purification Kits
Whole blood, buffy coat, plasma, body fluids and cultured cells
ready PCr dNa Column Kit - Purification on a filter-based column
10 Preps 733-1049P £18.00
ready PCr dNa Plate Kit – Purification on a filter-based 96 well plate
3 Preps 733-1056P £354.00
ready PCr dNa disk Kit – Purification on sample collection cards
10 Preps 733-1053P £18.00
Have you got your copy of the latest VWRbioMarke magazine?Full of technical and application articlesSee www.vwr.com
Molecular biology
ISSuE 9 - NOVEMBER 2007Shop!promo code
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4 Plates and Mats for Real-Time PCR and Sequencing• Plate wells have ultra-thin, consistent wall thickness giving precise thermal transfer
along entire surface of each well• all lots undergo the most rigorous Qa/QC procedures in the industry• Ensures consistent, reliable contamination-free performance• Plates available to fit most popular thermal cyclers• axyMats - re-usable to help reduce costs
Get 25% discount on accessories (mats and film)
Place your order today! Visit our website http://uk.vwr.comLocal price agreements apply.
offers valid from 19th November 2007 to 31st March 2008Promotions in the VWrbioMarke SHoP may not be used in combination, VWr reserves the right to refuse invalid orders.
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Order 5 packs but pay for only 4
The VWR Collection introduces a new, complete range of gel documentation systemsFeaturing the GenoSmart darkroom that allows the use of many bench top UV transilluminators - even one you might currently have in your laboratory. the system is compatible with most commonly used electrophoresis gels stains and by using a white or blue light converter you can also capture images from other applications such
as petri dishes, cells in flasks, autoradiographs, membranes, spot and slot blots of dNa, rNa or protein, tlC plates and more.
an easy-to-use “Stand alone”- instrument for fast documentation and analysis of dNa and protein gels. data can be transferred to PC via USB stick, leaving the unit free for the next user, whilst you work with your images.• real-time images from 8-bit CCd camera, (768 x 582 pixel resolution)• Self-contained, microprocessor controlled darkroom• integrated colour lCd screen for image viewing• White Epi-light• instant on-screen saturation detection• rapid photographic print production• transilluminator (optional)• White light converter (optional)
Contact your local VWr sales office for advice and a demonstration.
ThermoPrime™ PlusthermoPrime™ plus is a robust recombinant taq polymerase that can amplify fragments in excess of 12 kb, with enhanced thermal properties allowing for improved stability at room temperature. also available in the reddyMix™ format for direct loading on to electrophoresis gels. thermo-Start™ dNa Polymerase is a chemically modified taq Polymerase giving a true hot start enzyme. the chemical modification will give a sensitive, specific reaction with improved yields and reduced non-specific product. thermo Scientific dNtP’s have been developed to high purity standards and provide consistent results across a wide range of molecular biological techniques. available as a mix or separate, and in a variety of concentrations, these reagents will improve the performance of your PCr reaction.
* When purchased at list price.
Buy Taq DNA polymerase & get a
FREE pack of dNTP’s*
PCR Workstation™ designed to protect against contamination in sensitive PCR amplification reactions
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Easily remove rNase, dNase and dNa contaminates on pipettes, glassware, plasticware etc. with these convenient and effective wipes.
promo code5Stop contamination with RNase
AWAY® or DNA AWAY® Wipes
Buy 2 canisters & get a pack of
wipes free.
Complete monoclonal antibody developmentBy using improved mouse hybridoma technology under following conditions:• 0,2 mg purified protein for the complete project
(includes at least 2 clonings)• time frame 3-4 months • delivery of up to 10 different stable positive, igG producing clones • all project results and clones belong to the customer
*10% on first order through VWr BioScience sales people or direct through BioGenes
10% offfirst order*
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BioSciencespromo code
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Description Cat. No. Price
Gel documentation system GenoSmart includes digital video system with integral lCd monitor
730-3000P £3,213.00
Transilluminators
Size (cm) Wavelength (nm) Cat. No. Price
20 x 20 254 730-3017P £617.0020 x 30 254 730-3018P £746.0025 x 30 254 730-3019P £848.0020 x 20 254/ 365 730-3020P £920.0020 x 30 254/ 365 730-3021P £1,070.0025 x 30 254/ 365 730-3022P £1,220.0020 x 20 254/312 (302) 730-3023P £920.0020 x 30 254/312 (302) 730-3024P £1,070.0025 x 30 254/312 (302) 730-3025P £1,220.00
Cat. No. Dimensions (cm) UV type Price
732-1071P 61 x 61 x 76 single £1,970.00732-1072P 61 x 61 x 76 dual £2,030.00732-1073P 61 x 61 x 91 single £2,120.00
Cat. No. Dimensions (cm) UV type Price
732-1074P 61 x 61 x 91 dual £2,290.00732-1075P 61 x 61 x 122 single £2,720.00732-1076P 61 x 61 x 122 dual £2,880.00
Introductory prices on the GenoSmart
model
PCR Workstation™ features:• UV irradiation of working area prior to use blocks replication of contaminating
dNa sequences by causing adjacent pyrimidines to undergo dimerization• Protects against cross or airborne contamination by limiting exposure of
experimental set-up to open lab environment• three standard sizes available. dimensions are 61 cm (h) x 61 cm (d) with widths
of 76 cm, 91 cm and 122 cm• High-powered dual UV germicidal lamps with 12 hour countdown timer with
time-hold position• tempered safety glass fascia is hinged for easy access of interior workspace for
cleaning and placement of large instruments• duplex electrical outlet and two fluorescent lamps are mounted in a stainless
steel ceiling• acid-resistant black Formica work surface• Single or double access door(s) close to prevent contamination, then conveniently slide into storage compartment in base
during experimental procedures• Stainless steel ceiling increases UV dose on workstation surface • accessory table and accessory locking casters transform the PCr Workstation™ into a moveable work area that doesn’t
infringe on valuable bench-top space. available in two heights, 71 cm and 91 cm
Free replacement germicidal and
fluorescent bulbs with every purchase
Description Qty/Pk Cat. No. Price
rNase WaY® Canister 25 Wipes 115-1250P £34.00dNa aWaY® Canister 25 Wipes 115-1252P £34.00
Description Qty/Pk Cat. No. Price
No skirt, 300 µl 10 732-0661P £24.20No skirt, elevated wells, 300 µl 10 732-0662P £24.20No skirt – qPCr compatible, 300 µl 25 732-1024P £52.70No skirt, low profile, qPCr compatible, clear, 220 µl 25 732-1021P £45.20No skirt, low profile, qPCr compatible, black, 220 µl 25 732-1023P £52.70No skirt, low profile, qPCr compatible, white, 220 µl 25 732-1022P £52.70Segmented, 280 µl 10 732-0663P £24.20Elevated skirt, 300 µl 10 732-0665P £28.80Half skirt, low profile, qPCr compatible, clear, 220 µl 25 732-1015P £94.00Half skirt, low profile, qPCr compatible, black, 220 µl 25 732-1016P £94.00Half skirt, low profile, qPCr compatible, white, 220 µl 25 732-1017P £94.00Half skirt, qPCr compatible, 300 µl 10 732-0803P £28.80Full skirt , 200 µl 10 732-0664P £24.20
Description Qty/Pk Cat. No. Price Promo Price
Compression mat, 96 well, for real-time PCr 10 732-1020P £44.40 £33.30Sealing mat for 96-well PCr microplates 10 732-0668P £28.20 £21.1596-well silicone compression mat 10 732-0671P £28.20 £21.1596-well silicone expansion mat 10 732-0672P £28.20 £21.15UltraClear film, pressure sensitive 100 732-0657P £188.00 £141.00
Description Qty/Pk Cat. No. Price
thermoPrime® Plus dNa Polymerase 250 U 733-0020P £72.50thermo-Start™ dNa Polymerase 250 U 733-0059P £96.30dNtP Mix - 100mM 1 ml 733-0069P £102.00
ISSuE 9 - NOVEMBER 2007Shop!
Place your order today! Visit our website http://uk.vwr.comLocal price agreements apply.
offers valid from 19th November 2007 to 31st March 2008Promotions in the VWrbioMarke SHoP may not be used in combination, VWr reserves the right to refuse invalid orders.
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Optimise your immunoassaysMethods like Enzyme-linked immunosorbent assays (EliSa), Enzyme immunoassays (Eia), Western blotting, radioimmunoassays (ria), Protein-arrays, immunohistochemistry or the immuno-polymerase chain reaction (immuno-PCr) rely on immuno detection technology. Even in highly specific reactions, there is still the potential for tests to be disturbed by unwanted cross-reactivities.
Crossdown™ Buffer blocks most interference and results can be improved by using it in tandem with Blocking Solution i.
10% off
ThermoScientific Multiskan EX and Wellwash 4 Mk 2 - A proven solution for ELISA assays!
Get 15% off
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Nunc OptiCell® unique cell culture format for growing, monitoring and transporting cellsoptiCell® is a sterile, sealed cell environment between two optically clear gas-permeable growth surfaces in a standard microtitre plate-sized frame; self-sealing ports provide easy aseptic access to the interior and contents.
Key benefits• Saves time and money by reducing the steps and equipment needed for cell culture• reduces space and media requirements • Geometry optimises robotic handling• Compatible for use with standard, phase contrast, confocal, and high-resolution time-lapse video microscopes
The same tube you have always trusted with a NEW innovative cap design…
BD Falcon™ 50 ml Conical Tube with Flip-Top Capthis novel design saves time and effort for applications requiring multiple aliquoting, storage, and pouring from the same tube, while maintaining the same superior quality and performance of our standard screw-cap closure.
The Flip-Top Cap advantage:• Easy-to-use – one hand cap manipulation is virtually
effortless with the unique hinge action of the flip-top cap• Easy-to-read blue graduations on the tube allow rapid
volumetric assessment• Splash-Guard – clever design of the cap allows opening and closing of the cap
without risk of splatter• Secure seal – innovative click-bead ensures a tight seal every time• ambidextrous – thumb grips on each side of the cap ensure a sterile opening
technique.
laboratory wall clock22 x 24 x 1 cm
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Free blocking buffer
Detection of the antigen CaMK II in lysates of heart and brain tissue from C57Bl/6 mice. Left: TBS-Tween with 5 % nonfat dried milk. Right: CrossDown™ Buffer in combination with Blocking Solution I.(Data from Dr. Tronhung Quang, Dr. Rainer Klocke, Prof. Dr. Sigrid Nikol, University of Münster, Dept. Cardiology.)
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BD Falcon™ 50 ml Polypropylene Conical Tube with Flip-Top Cap: Simply flip the cap open and pipette your sample.
Free clock with every 2 cases purchased*
*offer limited to the first 50 customers – one clock per customer
Purchase either the Multiskan EX microplate reader or the Wellwash 4 Mk 2 microplate washer and get 15% off.
the unique design allows mixing horizontally, vertically or any position in between, with a simple turn of the rotisserie plate. the VWr Collection tube rotator has an ambient operating range of +4 ºC to 65 ºC and can be easily transported from the bench to a cold room, incubator or biological hood. the moulded housing and rotisserie assemblies are easy to clean. the speed is fixed to 20 rpm and the maximum load is 0,8 kg. Supplied with a rotisserie assembly that holds 36 x 1,5/2,0 ml tubes, with other sizes up to 50 ml available.
• Unique adjustable rotisserie assemblies
• Variable horizontal and vertical positioning
• Safe for cold room and incubator use
15% off rotator, 30% off accessories
Great value
The new VWR Collection tube rotator
Cell Biology
NEWRemove the burden of cleaning plastic
tanks in biopharmaceutical manufacturing
Buy now! Get 30% introductory discount
a complete line of NalGENE® tanks and matching tank liners ensure that your biopharmaceutical and diagnostic reagents can be prepared and mixed in a guaranteed clean container. the open bags with flat bottom design make fluid processing easy. Manufactured in co-extruded PE film, free of animal-derived components, these tank liners are non-cytotoxic, USP Class Vi & food-grade, and are offered as gamma-irradiated/non-pyrogenic or non-sterile.
these disposable NalGENE® tank liners allow our biopharmaceutical and diagnostic customers to upgrade the cleanliness of their mixing containers for their most valuable solutions. Eliminating the need for the validation of a tank cleaning process therefore reducing cost. all tank liners are individually bagged and are packed 10 units/case.
NALGENE Tank Liners
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Laboratory supplies
Plan your experiments in advance and get 45% discount on standard peptide services
Be smart - up to 45% off Without loss of quality!
NEW! Plan your experiments 4 to 7 weeks in advance and benefit from this new service. Simply mention the code VWr035 and if you are one of the first 50 orders you will also receive a free 128 MB USB-Stick.*
For more information refer to BioMarke magazine or email [email protected]. Thermo Scientific Biopolymers - Quality you can trust
* only first 50 ProjectPeptide™ orders mentioning the code VWr035 will receive a 128 USB Stick. USB stick not exchangeable for cash. offer valid from 1/11/2007 - 21/1/2008.
Adapt the service to your needs. Our solution: ProjectPeptides™
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128 MB USB-Stick for free*
Description Qty/Pk Cat. No. Price
Bd Falcon™ 50 ml polypropylene conical tubes with flip-top cap
440 734-1437P £93.80
BioSciences
Gamma irradiated 25-40 kGy
Tank Liner (non-sterile) (l)
Units/Case
Cat. No. Price Promo Price
19 10 216-2907P £184.00 £156.4028 10 216-2908P £193.00 £164.0538 10 216-2909P £197.00 £167.4557 10 216-2910P £203.00 £172.55113 10 216-2911P £306.00 £260.10208 10 216-2912P £330.00 £280.50303 10 216-2913P £356.00 £302.60378 10 216-2914P £378.00 £321.30568 10 216-2915P £468.00 £397.80757 10 216-2916P £538.00 £457.30
Tank Liner (sterile) (l)
Units/Case
Cat. No. Price Promo Price
19x 10 216-2917P £193.00 £164.05113 10 216-2918P £202.00 £171.70208 10 216-2919P £206.00 £175.10303 10 216-2924P £212.00 £180.20378 10 216-2925P £315.00 £267.75568 10 216-2926P £339.00 £288.15757 10 216-2927P £365.00 £310.25303 10 216-2928P £387.00 £328.95378 10 216-2929P £477.00 £405.45568 10 216-2930P £547.00 £464.95
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Non-sterile
Description Cat. No. Price Promo Price
VWr tube rotator, EU plug 444-0502P £189.00 £160.65assembly for 10 x 15/10 ml tubes and 16 x 5/7 ml tubes
444-0504P £23.45 £16.42
assembly for 6 x 50 ml tubes 444-0505P £23.45 £16.42
Description Qty Cat. No. Price
Crossdown buffer plus 50 ml blocking solution i free!
125 ml 999-9940P £95.60
Crossdown buffer plus 125 ml blocking solution i free!
500 ml 999-9941P £368.00
Description Pk Cat. No. Price Promo Price
optiCell® 1100 Starter kit 734-2221P £161.00 £120.7520 734-2222P £120.00 £90.00100 734-2223P £620.00 £465.00500 734-2224P £2,920.00 £2,190.00
optiCell® Max 2100 Starter kit 734-2225P £85.00 £63.7520 734-2226P £160.00 £120.00100 734-2227P £780.00 £585.00
Description Cat. No. Price Promo Price
Wellwash 4MK2 735-0438P £2,700.00 £2,295.00Multiskan Ex 735-0434P £3,140.00 £2,669.00
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ISSuE 9 - NOVEMBER 2007Shop!
Place your order today! Visit our website http://uk.vwr.comLocal price agreements apply.
offers valid from 19th November 2007 to 31st March 2008Promotions in the VWrbioMarke SHoP may not be used in combination, VWr reserves the right to refuse invalid orders.
these offers cannot be used in conjunction with any other pricing agreements from VWr international. Prices exclude Vat.
order from the Customer Service Centre or your VWr Primary distributor:
MediaKap® Hollow Fiber Media FiltersFor fast sterile filtration of media MediaKap® filters are designed to dramatically reduce the time required for sterilising and clarifying tissue culture media and other aqueous solutions. the filter comes in a full range of sizes with capacities from 2 to 50 litres. Spectrum offers two types of filters: MediaKap® and MediaKap® PlUS. the MediaKap® PlUS filters sterilise the same amount of dMEM in almost the same time but with 10% fetal bovine serum. the filter-fiber has a protein-binding of < 2 µg/cm2. radiation sterilised so no Eto residuals, all components pass U.S.P. XX1 class Vi toxicity test and the filters are non-pyrogenic by lal.
New AcroSepTM Chromatography Columns
the new acroSep™ Chromatography Columns for ion exchange, are ideal for optimisation studies of protein purification schemes using small sample volumes prior to scale-up and offer an efficient and reliable method for sample preparation of proteins for structural, functional and yield analysis.
the ion exchange columns contain Pall’s Hyperd chromatography sorbents which are created using patented “gel-in-a-shell” technology, resulting in a column which offers high dynamic binding capacities at fast flow rates for greater productivity at competitive resolution. the acroSep prepacked columns are made with a luer inlet and outlet for easy connection and versatile use:
• Fully automated - in combination with an automated chromatography instrument such as the ÄKta design* systems
• Semi-automated - in combination with pumps or manually with a syringe
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*while stocks last
promo code21Enhance your protein microarray applications
with Whatman FAST® SlidesFaSt® Slides are glass slides coated with a proprietary nitrocellulose polymer that maintains reactivity of proteins and ensures excellent, reproducible results. they offer significant advantages over traditional methods including improved sensitivity, linearity and quantitation. Many hundreds or thousands of antibodies or samples can be screened at the same time.
Free Soduku game with every
order
Features• Superior protein binding capacity• Highest sensitivity and dynamic range• Excellent long-term stability of printed proteins• Compatible with all detection methodologies• Compatible with commercially available
arraying robots
* Bar coded
Protein InvestigatorTM Protein investigator is a rapid, accurate and in-depth study of a protein from its sequence. the service includes the prediction of the structural and functional properties of your target, and will efficiently collect the maximum relevant information for your protein.
Money-back guarantee. If Protein Investigator does not bring you any useful information about your protein, you don’t pay!
BioSciences
ProteoExtract® Subcellular Proteome Extraction Kit
proteome fractions that are enriched in cytosolic, membrane/organelle, nuclear, and cytoskeletal proteins. in the case of adherent cells, the procedure is performed directly in the tissue culture dish without the need for cell removal. For suspension grown cells, extraction starts with gentle sedimentation and washing of cells. For tissues, the isolation of viable cells is required before proceeding with the extraction protocol.
the especially mild S-PEK procedure results in non-denatured proteins suitable for many downstream applications.
*one timer per order. offer valid while stocks last.
For full technical details of these, and other, sample preparation kits for protein research, please contact your local VWr sales specialist.
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Proteomics
the ProteoExtract® Subcellular Proteome Extraction Kit (S-PEK) is designed for reproducible extraction of subcellular
proteomes from mammalian cells. Based on different solubilities of certain subcellular compartments the S-PEK uses proprietary chemistries to yield four sub-
Free lab timer with any kit purchased*
Introducing BRAND´s new multichannel pipettesTransferpette® S-8/-12• Central pipetting button and separate tip ejector• Ergonomic finger rest for a relaxed grip for right- or left-
handed users• Effortless one-handed operation. Setting and locking
volume, pipetting, ejectings – all without shifting hand position
• Easy-to-read 4-digit volume display for highest precision• Complete autoclavability at 121°C (20 min.)• Easy calibration technique providing calibration
adjustments without tools, and a clear indication when the pipettes have been adjusted from factory settings
• Compatible with most common tips CE-iVd-compliant
Introductory offer!*Every transferpette® S -8 or transferpette® S -12 comes with one single-channel transferpette® S free!
NEW
Items supplied: Transferpette® S -8 or Transferpette® S -12, conformity certified, performance certificate, 1 filled Tip-Box N, 1 Tip-Rack refill unit, 1 shelf/rack mount, 1 reagent-reservoir, 1 set of sealing rings made of FKM
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No. of channels Surface Area (cm2)
Suggested Volume (l)*
Filling Bell
Qty/Pk
Cat. No. Price Promo Price
MediaKap®
MediaKap-2 35 0,2 - 2 Yes 12 734-0869P £63.00 £53.55No 18 734-0868P £80.50 £68.43
MediaKap-5 70 2 - 5 Yes 12 734-0871P £126.00 £107.10No 18 734-0870P £173.00 £147.05
MediaKap-10 100 5 - 10 Yes 12 734-0873P £189.00 £160.65No 18 734-0872P £267.00 £226.95
MediaKap-25 185 10 - 25 Yes 6 734-0874P £146.00 £124.10MediaKap-50 440 25 - 50 Yes 3 734-0875P £105.00 £89.25
MediaKap® PLUS
MediaKap-2 PlUS 35 0,2 - 2 Yes 12 734-3570P £95.60 £81.26
No 18 734-1324P £133.00 £113.05
MediaKap-5 PlUS 70 2 -5 Yes 12 734-0876P £157.00 £133.45
No 18 734-3571P £217.00 £184.45
MediaKap-10 PlUS 100 5 - 10 Yes 12 734-0877P £241.00 £204.85
MediaKap-25 PlUS 185 10 - 25 Yes 6 734-0878P £180.00 £153.00
MediaKap-50 PlUS 440 25 - 50 Yes 3 734-0879P £121.00 £102.85
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Multichannel Transferpette® S No. of channels
Volume (µl) Accuracy ≤ ± % Coefficient of variation ≤ %
Single-channel Transferpette® S (µl) included free!
Cat. No. Price
8 0,5 - 10 1,6 1,0 0.5 - 10 613-3710P £346.508 5 - 50 0,8 0,4 10 - 100 613-3711P £346.508 10 - 100 0,8 0,3 10 - 100 613-3712P £346.508 20 - 200 0,8 0,3 20 - 200 613-3713P £346.508 30 - 300 0,6 0,3 20 - 200 613-3714P £346.5012 0.5 - 10 1,6 1,0 0.5 - 10 613-3715P £399.0012 5 - 50 0,8 0,4 10 - 100 613-3716P £399.0012 10 - 100 0.8 0,3 10 - 100 613-3717P £399.0012 20 - 200 0,8 0,3 20 - 200 613-3718P £399.0012 30 - 300 0,6 0,3 20 - 200 613-3719P £399.00
Free USB vacuum cleaner kit with every
AcroSep order*
Description Colour Qty/Pk Cat. No. Price
CM Ceramic Hyperd F Green 5 569-1000P £64.60S Ceramic Hyperd F Sky Blue 5 569-1001P £64.60Q Ceramic Hyperd F red 5 569-1002P £64.60dEaE Ceramic Hyperd F orange 5 569-1003P £64.60
Description Dim. (mm) Qty/Pk Cat. No. Price
FaSt® Slides – 1 pad* 20 x 51 20 737-0000P £243.00FaSt® Slides – 2 pad* 20 x 20 10 732-0941P £146.00FaSt® Slides – 8 pad 6 x 6 10 732-0942P £166.00FaSt® Slides – 16 pad 6 x 6 10 732-0333P £185.00
FREE
* the suggested volumes for the MediaKap® PlUS filters are for serum-enriched media
-10% off
ProteoExtract® Subcellular Qty/Pk Cat. No. Price
Proteome extraction kit 20 rxn 539-9901P £258.00Proteome extraction kit, mini 10 rxn 539-9902P £137.00
VWR International LtdHunter BoulevardMagna ParkLutterworthLeics LE17 4XNt 0800 22 33 44f 01455 55 85 [email protected]
AGB Scientific Apps LTDA VWR International companyA10 Harbour Court7, Heron RoadSydenham Business ParkBelfast BT3 9HBt 028 9058 5800f 028 9080 7812
LAB3 LTDunit 1,Ross Road Bus. Centre,NorthamptonNN5 5AXt 01604 581 111f 01604 588 [email protected]
McQuILKIN & CO6/8, Rennie Place,College Milton North,East Kilbride G74 5HDt 01355 590 511f 01355 590 [email protected]
N E L S LTD7, Cumbie Way,Aycliffe Ind. Estate,Newton Aycliffe,Co Durham DL5 6YAt 01325 301 230f 01325 301 [email protected]
P&R LABPAK LTDunit 6, Ketterer Court,Jackson Street, St. Helens,Merseyside WA9 3AHt 0870 034 2055f 0870 034 [email protected]
R & L SLAuGHTER LTDunit 11-12,upminster Trading Park,Warley Street, upminster,Essex RM14 3PJt 01708 227 140f 01708 228 [email protected]