Shield One-Sheet 2019 v4 - LifeCanvas Technologies · SHIELD Exceptional Protection of Fluorescent...
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SHIELD Exceptional Protection of Fluorescent Proteins and Endogenous Molecular Information THE SOLUTION: Versatile: Safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture. Synergizes with a range of existing tissue processing approaches including SWITCH (CELL, 2015) and MAP (NBT, 2016). Easy & Reliable: Superior tissue preservation vs. CLARITY, without the variability of hydrogel embedding. Compatible with both non-fixed and PFA-fixed tissues. Fast: Streamlined protocol preserves samples in a matter of days with minimal steps. Multi-round imaging & staining: Protects tissue during FP-imaging and antibody labeling across multiple individual rounds. Available in 250mL and 500mL kits: Each kit comes with enough SHIELD Epoxy, SHIELD Buffer, and SHIELD ON solution to preserve 8 (250 mL kit) or 16 (500 mL kit) mouse brains or samples of similar size. SHIELD WORKFLOW: EFFECTIVE FOR BOTH UN-FIXED AND FIXED TISSUES Superior preservation of the most popular FPs PFA or PFA/SHIELD perfused tissue SHIELD epoxidation Optical-clearing and refractive index matching OR SHIELD postfixation 0 20 40 60 80 100 MeOH 4d GFP signal retention (%) TBA/ BABB 15m/1hr SDS 37°C 12hr SDS 56°C 12hr PFA SHIELD Organic Solvents CLARITY Improves signal in both CLARITY & organic solvent processed tissues Images & data from Park et al. 2018, Nature Biotech.
Transcript of Shield One-Sheet 2019 v4 - LifeCanvas Technologies · SHIELD Exceptional Protection of Fluorescent...
SHIELD Exceptional Protection of Fluorescent Proteins and Endogenous Molecular Information
THE SOLUTION: Versatile: Safeguards endogenous fl uorescence, protein antigenicity, nucleic acids, and overall tissue architecture. Synergizes with a range of existing tissue processing approaches including SWITCH (CELL, 2015) and MAP (NBT, 2016).
Easy & Reliable: Superior tissue preservation vs. CLARITY, without the variability of hydrogel embedding. Compatible with both non-fi xed and PFA-fi xed tissues.
Fast: Streamlined protocol preserves samples in a matter of days with minimal steps.
Multi-round imaging & staining: Protects tissue during FP-imaging and antibody labeling across multiple individual rounds.
Available in 250mL and 500mL kits:Each kit comes with enough SHIELD Epoxy, SHIELD Bu� er, and SHIELD ON solution to preserve 8 (250 mL kit) or 16 (500 mL kit) mouse brains or samples of similar size.
SHIELD WORKFLOW: EFFECTIVE FOR BOTH UN-FIXED AND FIXED TISSUES
Superior preservation of the most popular FPs
PFA or PFA/SHIELD
perfused tissueSHIELD epoxidation
Optical-clearing and refractive index matching
ORSHIELD
postfi xation
020406080100
MeOH4d
GFP
sig
nal
rete
ntio
n (%
)
TBA/BABB
15m/1hr
SDS 37°C 12hr
SDS 56°C 12hr
PFASHIELD
Organic Solvents CLARITY
Improves signal in both CLARITY & organic solvent processed tissues
Images & data from Park et al. 2018, Nature Biotech.
LIFECANVASTECH.COM
Facilitates antibody multiplexing across rounds
Images & data from Park et al. 2018, Nature Biotech.
Guiding Through Every Step of the Sample’s Journey
Enables robust fl uorescence in situ hybridization & immunohistochemistry
Images & data from Park et al. 2018, Nature Biotech.
