DIRECTOR'S COLUMN ...

Poultry production in Indiacontinues to grow at healthierrate (6-8% in layer and 10-

12% in broiler) and provides muchneeded animal protein at affordableprice to common man. However,this also generates sustained demandfor agricultural products such asmaize, soybean, sunflower meals, ricebran, etc. leading to higher pricesof these agricultural commodities,and therefore, the profit marginof poultry farming is under stress.We need to explore alternative ornon-conventional feed resourcesto bring down the cost of feedingin poultry. In a study conductedat DPR, it was demonstrated thatalternate feed resource such asacidulated soapstock, a by-productof vegetable oil refining industryhas been incorporated by replacingthe sunflower oil as the energysource with no adverse effects on theperformance of Krishibro broilers. Itindicates that this by-product couldbe used to reduce the expenditureon feed in Krishibro broiler farming.The protocol for gene knockdown ofmyostatin gene has been perfectedto enhance body weight in chicken.Backyard poultry is an importantcomponent of low input poultryfarming in the country for rural andtribal farmers. The performance ofPD-4 (Improved Aseel) birds withrespect to egg production up to 72

weeks of age was impressive underintensive type of management.Scientists demonstrated the activity ofMatrix metalloproteinases in chickenseminal plasma.Public Private Partnership wasemphasised as a way forward forresearch and development field. DPRhas entered in to as many as fourmemorandums of understandingwith private firms for providingconsultancy and research servicesduring the period. Notable are thevalue addition to alternative feedresources and diagnostic services forInfectious Bronchitis virus. DPR stallcontinues to attract visitors at variousexhibitions conducted in the period.Director joins all the staff to wish aHappy New Year - 2015 for readers ofthe ICAR-DPR News letter.

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July- December 2014

Congratulations to• Dr. R.N.Chatterjee, Director for

beeing elected as NAASFellow

• Dr. K.5. Rajaravindra, Scientistfor being awarded first prizefor oral presentation at theNational Conference on NativeChicken Production, Chennai

• Dr. A. K. Panda, PrincipalScientist for being plenaryspeaker at lO" Asia PacificPoultry Conference at Jeju,South Korea

Mr. D. Srinivas for winningsecond prize in the Chesscompetition in ICAR Southzone sports meet

IN THIS ISSUEDirector's column

Research highlights 2

Events organised 3

Transfer of technology 4

Meetings conducted 5

Participation in symposia/conferences/ sem inars/workshops 6

Talks delivered 8

Awards/ recognitions 8

Distinguished visitors 8

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RESEARCH HIGHLIGHTS

Technique to knock-down myostatin gene inchicken perfectedA total of 5 shRNA molecules located at 194, 21S, 29S, SII and926 nucleotide down-stream of the initiation codon of myostatin(MSTN) gene with GC% varied from 47 to 52% were designedand cloned in pENTR U6 entry vector. The shRNA moleculeswere tested in vitro in chicken primary myoblast culture fortheir relative efficiency and found that all the molecules hadsilencing efficiency up to 60% for expression of MSTN gene. Allthe shRNA molecules were transferred through embryo as wellas spermatozoa using DMSO, High temperature (HT) semendiluent and PBS by gene pulser and lipofectamine mediatedmethod. The effect of these molecules on post-hatch bodyweight was analyzed and found Significantly (P<0.05) higherbody weight at 4, 6 and S weeks of age. The molecule 5 showedIS.S, 37.S, 25 and 33 % higher body weight than the birds ofcontrol group at 2, 4, 5 and 6 weeks of age respectively. At 6weeks, molecules 3, 1,2 and 4 showed 26.9, 22, 23.4 and 26.4 %higher body weight than the birds of control group, respectively.The comparison of different methods revealed that gene pulserwith HT diluent for transfection to spermatozoa showedsignificantly highest body weight at 6 weeks of age. This methodwas followed by DMSO simple incubation, gene puls er withPBS and lipofection. This method of knocking down the genemay be used for silencing other deleterious genes to improveproductivity and health of chicken.

T.K. Bhattacharya, R. Shukla,R.N. Chatterjee and K. Dushyanth

Acidulated soapstock as an energy source in thediet of broiler chickensFats/oils are most valuable source of energy in poultry diet.Since oils are expensive, there is continuous search for alternateenergy sources in order to reduce the cost of production. Onesuch energy source is acidulated soapstock (AS), which is aby-product of vegetable oil refining industry and is essentiallya good source of energy and essential fatty acids. The presentstudy was therefore conducted to evaluate the effect ofacidulated soapstock as an energy source in the diet of broilerchicken. Day old broiler (Krishibro) chicks were wing bandedand randomly distributed into 40 raised-floored stainless steelbattery brooder pens with 5 birds per pen. Basal starter (0-3week, 2900 kcal ME/kg and 22% CP) and finisher (4-6 week,

3000 kcal ME/kg and 20% CP) diets were prepared usingsunflower oil (SFO) as an energy source. Subsequently, 3 testdiets were prepared in which SFO was replaced with acidulatedsoapstock at 25, 50 and 100% level. Each diet was fed at randomto 10 replicates of 5 chicks each throughout the experimentalperiod (0-6 week). The body weight and feed consumptionparameters were recorded on weekly basis. At the end of theexperimental period, 10 birds from each dietary group wereslaughtered to record the carcass traits. Dietary replacement ofSFO with AS partially (25 or 50%) or completely (100%) hasno influence on either body weight gain or feed conversionratio. The carcass characteristics such as dressed weight, breastmeat yield, giblet weight and abdominal fat contents were alsonot influenced by the replacement of SFO with AS in the diet.From the results of the present study, it can be concluded thatacidulated soapstock can be used as an energy source in thediet of Krishibro broiler chickens.

A.K. Panda, B. Prakash,S.V. Rama Rao and M. V.L.N. Raju

Production performance of PD-4 birds up to72 weeks of ageProduction performance of PD-4 birds (Improved Aseel)was evaluated up to 72 weeks of age. It was observed thatsurvivors' egg production up to 72 weeks of age was 156.5±2.79eggs while hen day (HDEP) and hen housed egg production(HHEP) were 144.9 and 137.5 eggs respectively. Survivors' eggproduction recorded up to 40 weeks of age was 60.S± 1.32. Eggproduction started at 21 weeks and peaked during 33-36 weeksand thereafter it declined slowly up to 65-6S weeks but againincreased marginally during 69-72 weeks of age. Egg weightconsistently improved from 35.13 g at age at first egg to 4S.07g at 40 weeks of age. However, it has increased by 1.56 g from40 weeks of age to 72 weeks of age (49.63±0.43). Body weightand shank length of hens at 72 weeks of age were 1949±19.05g and 104.5±0.31 mm respectively. There was moderateimprovement in body weight from 40 weeks of age to 72 weeksof age (1949±19.1).

S. Haunshi, U. Rajkumar, M.K. Padhi, and M. Niranjan

Matrix metalloproteinase (MMP) in chickenseminal plasmaMatrix metalloproteinases (MMPs) are large family of zinc-dependent proteinases that are involved in degradation ofprotein components of the extracellular matrix. MMPs havebeen reported to be present in the seminal plasma of some

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species but there is no report of its presence or function in avian delivered a lecture on Poultry Industry in which students andseminal plasma. MMP-2 a 72 kDa protein has been detected scientists interacted with industry people.in the seminal plasma of Dahlem Red chicken by gelatinzymography indicating its possible role in fertility of birds.

Human MMP marker

Pro MMP-2

MMP-2

Gelatin zymography in chicken

M. Shanmugam, A. Vinoth,K.S. Rajaravindra, and U. Rajkumar

Effect of organic chromium on breeder semenquality, fertility and hatchability in PD-3lineChromium (Cr), a naturally occurring element in trivalentform has been shown to play important role in glucose, fatand protein metabolism. Supplemental Cr has been reportedto improve semen quality, fertility and hatchability in otherspecies. A feeding experiment was carried out to evaluate theeffects of supplemental organic Cr on semen quality, fertilityand hatchability in PD-3line. A total of 40 PD-3 roosters of 22weeks age were equally distributed in to four groups and dietswere supplemented with organic Cr at graded levels (0, 0.3, 0.6,0.9 mg/kg) for 12 weeks duration. Semen collected after 4 and8 weeks of experiment was analysed for different gross semenparameters and seminal plasma lipid peroxidation. A fertilitytrial was conducted for 4 weeks duration where fixed dose ofsperm (lOOmillion in O.lml) was inseminated every week. Theeggs collected were incubated and fertility and hatchabilitydata was collected. The results indicated no significant effectof supplemental Cr on different parameters studied. It can beinferred that Cr supplementation in diet does not improvebreeder semen quality, fertility and hatchability in PD-3 line.

M. Shanmugam, B. Prakash and A. K. Panda

EVENTS ORGANISED

ICAR Foundation Day celebratedDPR celebrated ICAR's Foundation Day on 16th July 2014 at theconference hall of the DPR, Hyderabad. Dr. R. N. Chatterjee,Director presided over the function. On the occasion, Poultryindustry-students interface meeting was organized in whichDr. G. Ranjit Reddy, M.D., S.R. Hatcheries Pvt. Ltd, Hyderabad

ICAR Foundation day celebration

Independence Day celebratedDPR celebrated Independence Day on 15th August 2014. TheDirector hoisted the National flag and addressed the gathering.On the occasion, he narrated the great contribution of thefreedom fighters for the Independence. He also highlighted theresearch and development efforts of DPR and impacts of itstechnologies on the livelihood of poor farmers in the country.

Hindi implementation activitiesThe Directorate conducted two quarterly meetings of OfficialLanguage Implementation Committee (OLIC) on 28th August2014 and 16th December 2014, in which different issues relatedto effective implementation of Hindi Language in office werediscussed. The Directorate also conducted two Hindi workshopson 2nd September and 29th December 2014 for upgrading the

DPR

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Hindi skills of staff in day to day official work. The Directoratealso carried out Hindi Fortnight celebrations during l " to 15th

September 2014. During these celebrations different literarycompetitions were conducted for the staff. Hindi Day on 14thSeptember 2014 was observed and Shri Brihaspati Sharma,Retired Manager (OL), Punjab National Bank, Hyderabadgraced the occasion as the chief guest and he highlighted theimportance of Hindi language and its history. All the winners/runners of different competitions were awarded with cashprizes and certificates on this occasion.

Hindi Day celebration in progress

Swachh Bharat AbhiyanKeeping in view the launching of Swachh Bharat Abhiyanby Hon'ble Prime Minister of India, all the employees of theInstitute actively participated on every Wednesday of eachmonth from 2nd October to 31't December 2014. Monthly planand five year plan of cleaning have been prepared in order toachieve the goal of Swachh Bharat set by our Hon'ble PrimeMinister by October, 2019.

Vigilance awareness weekVigilance awareness week at the Institute was observed from27th October to l " November 2014 by taking the pledge.

TRANSFER OF TECHNOLOGY

DPR distributed improved chicken varietiesA total of 320 grown up chicks of 6 weeks old were distributedto tribal farmers (40 No.) of Sarlapur and Kudichintala biluvillages in Mahabubnagar district of Telangana state. Prior tothe distribution, the farmers were appraised about advantagesof rearing improved chicken varieties. The scientist fromDPR is regularly monitoring the work in collaboration withDirectorate of Oilseed Research. Dr. R.N. Chatterjee, Director,DPR along with the team of scientists visited the villages andexpressed satisfaction about the performances of the birds.

DPR stall attracts visitors at Poultry India 2014DPR participated in Poultry India 2014 exhibition organized byIPEMA at Hitex, Hyderabad from 26th to 28th November 2014.DPR stall attracted the attention of all the delegates and poultryfarmers. The technologies developed by the Institute especiallythe improved chicken varieties; Vanaraja, Gramapriya andSrinidhi attracted the poultry farmers. About five thousandfarmers, technocrats and scientists visited the stall in 3 days.

DPR participated in farmers' day at DRR,HyderabadDPR Participated in the farmer's day organized by Directorateof Rice Research, Hyderabad on 15th November 2014. DPRstall attracted the attention of the farmers and visitors at theexhibition. The literature on the improved chicken varietieswas distributed to the farmers.

Cleaning of farm premises is under progress During the programme, technical advises and inputs on rural!backyard poultry farming, disease management, commercial

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poultry production and related poultry activities were providedto the farmers from various parts of the state and other states also.

Public- Private PartnershipA total of 4 Memorandums of Understanding (Mo U) weresigned between the Directorate of Poultry Research andsponsoring organizations during the period.

MoU was signed between DPR, Hyderabad and AbhayCotex Pvt. Ltd., [alna, Maharashtra on 18th July 2014 forcontract research project on "Enhancement of nutritionalvalue of alternate protein sources in broiler and layer diets"for one year.MoU was signed between DPR, Hyderabad and OrffaAnimal Nutrition Pvt. Ltd, Thane (W), Maharashtraon 31st July 2014 for contract research project on "Effectof different emulsifiers on the dietary fat digestibility,metabolisable energy and production performance inbroiler chicken" for six months.

•

MoU was signed between DPR and Intervet India Pvt. Ltd.,Pune, Maharashtra on 23rd September 2014 for contractresearch project on "Infectious bronchitis (IB) virus isolationand molecular identification from field samples" for two years.

MoU was signed between DPR and Sri Ramadhootha PoultryResearch Farm, Kothur village, RR District, Telangana on25th July 2014 for Advisory consultancy project on "Technoscientific advisory services to organized poultry researchfarm on nutrition and health care" for one year.

MEETINGS/WORKSHOPS/TRAININGSCONDUCTED

Institute Research Committee MeetingHalf yearly Institute Research Committee meeting was held onl" November 2014. Principal investigators of various projectspresented the achievements. Chairman IRC suggested themeasures to overcome the difficulties in achieving desired targets.

Half yearly IRe meeting in progress

Research Advisory Committee MeetingThe 8th Meeting of the Common Research Advisory Committeeof Central Avian Research Institute (CARl), Izatnagar andDirectorate on Poultry Research (DPR), Hyderabad was heldon 22nd July 2014 at CARI, Izatnagar under the Chairmanshipof Dr. R. Prabakaran, Ex-Vice Chancellor, Tamil NaduVeterinary and Animal Sciences University, Chennai. Dr. R.N.Chatterjee, Director presented the overall achievements ofDPR, Hyderabad. He narrated the profile and contribution ofthe Institute to the farmers and Industry in the country. Dr.S.v. Rama Rao, Principal Scientist; Dr. M.K. Padhi, PrincipalScientist,; Dr. T.K. Bhattacharya, National Fellow and Dr. M.R .Reddy, Principal Scientist presented the progress of researchof Nutrition & Physiology, Breeding, Biotechnology andHealth sections of the Institute respectively. Dr. Prabakaran,Chairman appreciated the scientists of DPR for the overallresearch achievements made during the period.

'Annual Review Meeting of AICRP on PoultryBreeding and Poultry Seed ProjectAnnual Review Meeting of AICRP on Poultry Breedingand Poultry Seed Project for the year 2013-14 was held atDirectorate of Poultry Research, Hyderabad during 22nd & 23rd

August 2014. Prof. K.M.L. Pathak, DDG (Animal Science) wasthe Chief Guest while Dr. R.S. Gandhi, ADG (AP&B) was theGuest of Honour. Prof. K.M.L. Pathak, released the AnnualReport of AICRP on Poultry Breeding and PSP for the year2013-14. He also released a technical bulletin entitled "RuralPoultry Production: A technical bulletin for higher sustainableproduction" authored by the Scientists of Agartala centre ofAICRP. Dr. R.S. Gandhi released research highlights of AICRPon Poultry Breeding and PSP for the year 2013-14. DDG (AS) inhis address complemented DPR for getting ICAR OutstandingInstitution Award and said that the award is the reflection ofthe hard work of the scientists and staff of the Directorate. Dr.R.S. Gandhi, also complemented Director and staff of DPR for

r getting the Sardar Patel Outstanding ICAR Institution Award(Small institute category). He requested all the AICRP centresto participate in RSPPT and to initiate pedigree hatching ofnative germplasm collected by respective centres. Dr. R.N.Chatterjee, Director, DPR briefed about the achievements ofAICRP on Poultry Breeding since inception. He narrated aboutthe development of rural poultry and role of AICRP and PSP inenhancing poultry production in the country. All the centre In-charges presented the progress report of their respective centresand discussion was made on achievements, shortfalls and futurestrategy for the coming year both for AICRP and PSP.

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Annual review meeting of AICRP on Poultry Breeding andPoultry Seed Project in progress

Institute Joint Staff Council (IJSC) MeetingThe 2nd meeting of 8th Institute joint staff council (IJSc) washeld on 20th November 2014 at this Directorate for the periodfrom October to December, 2014.

Institutional Biosafety Committee MeetingThe 5th Institutional Biosafety Committee meeting was heldon 30th August 2014 under the chairmanship of Dr. R.N.Chatterjee, Director. Dr. Aparna Dutta Gupta, Professor, I

University of Hyderabad and DBT Nominee was present in themeeting. The issues related to biosafety of the ongoing as wellas new research projects were deliberated.

Institutional Animal Ethics Committee MeetingThe 15th and 16th Institutional Animal Ethics Committee

meetings were held on 5th July 2014 and 19th September 2014.

Model Training ProgrammeDirectorate of Poultry Research, Rajendranagar, Hyderabadorganized an eight days training program on 'Amelioratingthe Effects of Climate Change on Poultry Production' tothe Veterinary officers from Assam, Orissa, Uttar Pradesh,Himachal Pradesh, Sikkim, Andhra Pradesh, Tripura andTelangana states during I" to 8th September 2014. The trainingprogramme was sponsored by Department of AgriculturalExtension, Government ofIndia. A total of twenty Veterinariansfrom various states participated in this training program. Theparticipants were exposed to various activities related to poultryfarming with more emphasis on overcoming the adverse effectsof impending climate change on poultry.

Director and resource persons along with trainees

PARTICIPATION IN SYMPOSIA/CONFERENCES/SEMINARS/WORKSHOPS

SI. Symposia / conferences/ seminars /meetings / Scientist( s) /Officer( s) Duration Venue

No.workshops

1 International Conference on Dr. R.N. Chartterjee, Director 12th -15th July 2014 National InstituteHost Pathogen Interaction Dr. M. R. Reddy, Pr. Scientist of Animal

Dr. Santosh Haunshi, Sr. Scientist Biotechnology,Dr. KS. Rajaravindra, Scientist Hyderabad

2 National Conference on Dr. R.N. Chartterjee, Director 4th & 5th September Madras VeterinaryNative Chicken Production: Dr. M.K Padhi, Pr. Scientist 2014 College, ChennaiChallenges and Opportunities Dr. U. Rajkumar, Pr. Scientist

Dr. Santosh Haunshi, Sr. ScientistDr. KS. Rajaravindra, Scientist

3 Annual Review Meeting of Dr. R.N. Chatterjee, Director 18th - 20th Directorate ofA.I.C.R.P. on Poultry Breeding Dr. S.Y. Rama Rao, Pr. Scientist September 2014 Poultry Research,& Poultry Seed Project Dr. M.Y.L.N. Raju, Pr. Scientist Rajendranagar,

Dr. B.L.N. Reddy, Pr. Scientist HyderabadDr. M.K Padhi, Pr. ScientistDr. T.K Bhattacharya, National Fellow

Dr. M.R. Reddy, Pr. Scientist

Dr. M. Niranjan, Pr. Scientist

Dr. U. Rajkumar, Pr. Scientist

Dr. A.K Panda, Pr. Scientist

Dr. R.K Mahapatra, Sr. Scientist

Dr. Santosh Haunshi, Sr. Scientist

Dr. T.R. Kannaki, Scientist

Dr. K S. Rajaravindra, Scientist

Dr. S.K Bhanja, CTO4 loth Asia Pacific Poultry Dr. A.K Panda, Pr. Scientist 19th - 23rd October ICC, [eju, South

Conference 2014 Korea5 2nd Annual Conference and Dr. R.N. Chartterjee, Director 7th & 8th November Pune

National Symposium of Dr. M.Y.L.N., Raju, Pr. Scientist 2014

Association of Avian Health Dr. M.K Padhi, Pr. Scientist

Professionals Dr. M.R. Reddy, Pr. Scientist

Dr. M. Niranjan, Pr. Scientist

Dr. U. Rajkumar, Pr. Scientist

Dr. A.K Panda, Pr. Scientist

Dr. T.R. Kannaki, Scientist

Dr. KS. Rajaravindra, Scientist

Dr. S.K Bhanja, CTO6 Knowledge Day Seminar by Dr. R.N. Chartterjee, Director 25th November Novotel, Hitech

Poultry India Dr. M.Y.L.N. Raju, Pr. Scientist 2014 City, Hyderabad

Dr. M.K Padhi, Pr. Scientist

Dr. M.R. Reddy, Pr. Scientist

Dr. M. Niranjan, Pr. Scientist

Dr. U. Rajkumar, Pr. Scientist

Dr. A.K Panda, Pr. Scientist

Dr. R.K Mahapatra Sr. Scientist

Dr. B. Prakash, Scientist

Dr. KS. Rajaravindra, Scientist7 31st Annual Conference Dr. M.R. Reddy, Pr. Scientist 13th _ 15th Anand Agricultural

of India Association of November 2014 University, Anand

Veterinary Pathologists8 31st Annual Conference Dr. B.L.N. Reddy, Pr. Scientist 18th _ 20th Veterinary College

of Indian Poultry Science Dr. R.K Mahapatra, Sr. Scientist December 2014 and Research

Association and National Dr. M. Shanmugam, Scientist Institute, Namakkal

Symposium on "Poultry Dr. KS. Rajaravindra, Scientist

production for Global Trade" Dr. S.K Bhanja, CTO

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DPR

TALKS DELIVERED• Dr. R.K Mahapatra, Sr. Scientist delivered a seminar on

"Poultry semen diluents and storage: an overview" as a partof monthly seminar series on 18th August 2014.

• Dr. TR. Kannaki, Scientist delivered a talk on "Geneticmapping of host genes associated with Marek's diseaseresistance in chicken" on loth September 2014 on account ofundergoing DBT-CREST award training at Roslin Institute,Midlothian, United Kingdom.

• Dr. K Sushitha, Scientist and Ph.D. Scholar delivered aseminar on "Pathology of Pathogenic Avian Mycoplasma"on 14th November 2014.

DISTINGUISHED VISITORS1. Prof. KM.L. Pathak, DDG (Animal Science), ICAR, New

Delhi2. Dr. S.P.S.Ahlawat, Former Director, IVRI, Izatnagar3. Dr. R.S. Gandhi, ADG (AP&B), ICAR, New Delhi4. Dr. S.K. Diwedi, Former Director, NRC Equines &

Chairman, QRT-DPR5. Dr. KT Sampath, Former Director, NIANP, Bangalore6. Sixty eight final year B.Y.Se. & A.H. students along with two

Professors from VCRI, Namakkal, Tamil Nadu visited DPR •on 3rd November 2014

7. Dr. David E. Swayne, USDA, Athens, Georgia, USA visitedDPR during November 2014

INTERNATIONAL DIGNITARIESVISITEDDPR

Minister of State for Livestock, Ethiopia visitedDPRLivestock minister of Ethiopia along with team of experts visitedthe Directorate of Poultry Research on 25th October 2014.They were appraised about the activities of the Directorate andtechnologies developed at the Institute. They appreciated the

Edited byDr. Santosh Haunshi, Sr. ScientistDr. M. Shanmugam, ScientistSri J. Srinivas Rao, Sr. Technical OfficerDr. TK Bhattacharya, National Fellow

efforts of the institute for upliftment of the poor rural peopleof the countr .

B.V.Se. & A.H. students along with Professors from VeRI, Namakkal

PERSONALIA• Dr. M.K Padhi, Pr. Scientist was transferred to Regional

Centre, CARI, Bhubaneshwar w.eJ. 12th December 2014 onpersonal request.

AWARDS/RECOGNITIONS• Dr. R.N. Chatterjee, Director has been elected as NAAS

Fellow.Dr. KS. Rajaravindra, Scientist awarded first prize fororal presentation for the paper entitled "Genetic diversityof mitochondrial genes in native chicken breeds", at theNational Conference on Native Chicken Production:Challenges and Opportunities held at Madras VeterinaryCollege, Chennai during 4th & 5th September 2014.

• Dr. A.K Panda was invited by the loth Asia Pacific PoultryConference organizing committee, as a Plenary Speaker forAPPC 2014 which was held from 19th - 23rd October 2014in [eju Island, South Korea. He attended the conference andchaired the plenary session III and also delivered a plenarylecture on "Feed Additives Trend in Poultry Nutrition':

• Mr. D. Srinivas, skilled support staff won second prize in theChess competition in ICAR South zone sports meet held atBengaluru during 13th to 17th October 2014.

To

Published by Director

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ICAR - Directorate of Poultry ResearchRajendranagar, Hyderabad - 500 030, Telangana, India.

Ph.: +91 (40) 24015651/7000/5652/8687 Fax: +91 (40) 2401 7002 Email: pdpoult@nic.inWebsite: www.pdonpoultry.org

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