Session 6: Combining monitoring and incursion surveillance for grains

biosecurity built on science

Project 2113 Combining monitoring and incursion surveillance for grains

Mark StevensPrincipal Research Scientist, NSW DPI, Yanco

Plant Biosecurity Cooperative Research Centre


The original objectives

• Can we monitor multiple species of stored product beetles on a single trap without adverse pheromone interactions (Rhyzopertha / Tribolium / Sitophilus / Cryptolestes)?

• Can we monitor simultaneously for an exotic pest (larger grain borer Prostephanus truncatus)?

• WHY? If we can, then we get more efficient population monitoring / sampling for assessment of phosphine resistance / early detection of a PT incursion.


The evolved (additional) objectives

• Can we make the Cryptolestes ferrugineus pheromone lure work more effectively?

• Can we make commercial Sitophilus oryzae pheromone lures work outdoors AT ALL ?


Strategy and results - interactions

• Interactions: Lindgren 4-unit funnel traps, 300 m circle, 4 groups of 4 or 6. Pairwise comparisons then individual lures vs 4 lures together.

• Commercial Trécé lures for Rhyzopertha, Tribolium and Prostephanus, Research Directions lure + 3 release tubes of ‘volatile X’ for Cryptolestes.


Strategy and results: 2-way interactions• Most pairwise comparisons look

like this:• Except this one:


Strategy and results: 4-way interactions

• A component of the Cryptolestes combined lure seems to increase the attraction of Rhyzopertha.

• Nothing much else going on in terms of interactions.

• But we don’t have Prostephanus here – so work to start in South Africa this spring.


Better performance from the Cryptolestes lure

• Volatile X caught > 11 times the number of C. ferrugineus than the pheromone lure.

• Combining volatile X and the pheromone lure led to > 2.8 times the catch of volatile X alone.


• More units on the LFT didn’t make a difference.• More C. ferrugineus caught on the panel trap, probably dues to higher volatile X

emission rates.

Better traps for Cryptolestes


• Trials in South Africa to be run by Prof. Johnnie van den Berg this season – do Rhyzopertha / Tribolium / Cryptolestes pheromones affect the response of Prostephanus to its pheromone?

• Can we make Sitophilus oryzae lures work? Two choice pitfall data suggests it may be possible.

What happens next?


What happens next?

Table 2. Response of adult S. oryzae to volatile organic compounds in two-choice pitfall bioassays. Compound Response Index (Mean ± SE)a

Total Males Females

Control (pentane vs blank) -1.84 ±11.39 -0.98 ±11.41 -6.58 ±12.38

1-octen-3-ol +32.02 ±7.99* +31.31 ±11.01* +35.80 ± 9.37* trans-3-octen-2-one +31.57 ±11.24* +36.61 ±13.28 +30.77 ±12.12 hexanoic acid +7.17 ±13.14 +14.51 ±13.71 -2.74 ±15.77 nonanal +13.59 ±9.01 +19.36 ±11.83 +11.09 ± 8.54 2-ethyl-1-hexanol +19.92 ±9.46 +23.60 ± 9.04* +14.32 ±12.37 4’-ethylacetophenone +1.26 ±13.03 +3.07 ±16.29 +4.16 ±12.69 3-methyl-1-butanol +43.91 ±8.60** +44.45 ±10.23** +42.14 ± 7.97** 3-octanone +7.54 ±10.43 +9.68 ± 7.68 +4.09 ±14.52 2-phenylethanol +9.44 ±10.82 +13.50 ±10.88 +4.83 ±11.90 valeraldehyde -30.42 ±7.02*** -30.14 ±6.63** -29.57 ±10.78**


End-users?

• For more information, please email [email protected]

• Grain growers and handlers wanting to monitor pest populations to optimise timing of grain handling operations and/or sample populations for resistance testing, whilst also contributing to incursion surveillance (Prostephanus).

• Researchers conducting ecological studies wishing to monitor multiple species more efficiently (by avoiding the need to use one trap per species).

• The future – efficient incursion monitoring for multiple exotics on single traps?

mailto:[email protected]

Session 6: Combining monitoring and incursion surveillance for grains

Science

Transcript of Session 6: Combining monitoring and incursion surveillance for grains