Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department...

34
1 Department of Chemistry, University of Wisconsin-Madison 2 Department of Chemistry, University of Virginia 3 National Institutes of Health 4 Thermo Electron, San Jose CA Sequential ion/ion reactions for large peptide and whole protein characterization Joshua J. Coon, 1 An Chi, 2 Beatrix Ueberheide, 2 Jeffrey Shabanowitz, 2 Melanie J. Schroeder, 2 Lewis Y. Geer, 3 Dina Bai, 2 Donald F. Hunt, 2 and John E. P. Syka 4

Transcript of Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department...

Page 1: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

1Department of Chemistry, University of Wisconsin-Madison2Department of Chemistry, University of Virginia

3National Institutes of Health4Thermo Electron, San Jose CA

Sequential ion/ion reactions for large peptide and whole protein

characterization

Joshua J. Coon,1 An Chi,2 Beatrix Ueberheide,2 Jeffrey Shabanowitz,2 Melanie J. Schroeder,2 Lewis Y. Geer,3 Dina

Bai,2 Donald F. Hunt,2and John E. P. Syka4

Page 2: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 3: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 4: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 5: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 6: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 7: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

B

ESIESIe-

multiply-protonatedpeptides

Page 8: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 9: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 10: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 11: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 12: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

+

Page 13: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

++ +

Page 14: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

+

Page 15: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

+

-

Page 16: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

+ - - -

Page 17: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

-

+

Page 18: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University

--

+

Page 19: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 20: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 21: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 22: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 23: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 24: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 25: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 26: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 27: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 28: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 29: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 30: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 31: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 32: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 33: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University
Page 34: Sequential ion/ion reactions for large peptide and whole protein … · 2010-08-11 · 1Department of Chemistry, University of Wisconsin-Madison 2Department of Chemistry, University