Separating Ferrocene and Acetylferrocene by adsorption column chromatography
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Transcript of Separating Ferrocene and Acetylferrocene by adsorption column chromatography
Separating Ferrocene and Acetylferrocene by adsorption column chromatography
AcetylferroceneFerrocene
Adsorption column chromatography
In Column chromatography, the stationary phase (absorbent) is packed in a glass column
The mobile phase (solvent), moves through the packed column
The solvent that is used as the mobile phase is called an eluent
Atracttion
A mixture of compounds is added to the eluent As the eluent moves through the column the mobile
phase and stationary phase interact with the compounds.
If a compound is more attracted to the mobile phase it will move rapidly through the column and Elute, (leave)
If a compound is more attracted to the stationary phase it will move slower.
Atracttion
The more polar compounds in the mixture will bind strongly to the absorbent : less polar compounds not so strong.
Separtion occurs when an eluent with a low to moderate polarity is passed through the column.
The less polar compounds in the mixture will move throught the column
The strongly polar compounds will remain with the absorbent.
Polarity of eluents
In some cases two eluents maybe used, the eluent with the lowest polarity is used first
Hexane Tetrachloroethane Benzene Toluene Trichloroethane Dichloroethane Diethylether Tert-butyl-methyl ether Ethyl acetate Acetone Ethanol Methanol water
INCREASING
POLARITY
Ferrocene = Yellow
Acetylferrocene = orange