Seminar 07
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Seminar 07 Spine-Type-Specific Recruitment of Newly Synthesized AMPA Receptors with Learning
description
Seminar 07. Spine-Type-Specific Recruitment of Newly Synthesized AMPA Receptors with Learning. nuclei TO-PRO-3 |anti-GFP antibody. DOX (+) Home cage. DOX (+) 24 hrs FC. DOX (-) Home cage. DOX (-) 24 hrs FC. Novel context alone can induce c-fos promoter in CA1 field. - PowerPoint PPT Presentation
Transcript of Seminar 07
Seminar 07
Spine-Type-Specific Recruitment of Newly Synthesized AMPA Receptors
with Learning
DOX (+)Home cage
nuclei TO-PRO-3 |anti-GFP antibodyDOX (+)
24 hrs FCDOX (-)
Home cageDOX (-)
24 hrs FC
Novel context alone can inducec-fos promoter in CA1 field
DiI crystals to label dendrites
New GluR1 found onsome dendritic spine heads
Anatomical classification of spines
FC transiently increases % mushroom spines with new GluR1
No difference between apical and basal CA1 dendrites
Extinction protocol
Extinction of contextual FC
Extinction learning actually prolongs requirement for new GluR1
No changes in the density of spines or the distribution of spine types
