SeLumba)B.)Mukasa2,)Anton)Bua1andNigelTaylor 3 ·...
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Produc’on of and Regenera’on of Friable Embryogenic Callus and Apical Shoot Tips in Ugandan Farmer Preferred Cassava Genotypes SP0901 Hellen. B. Apio 1,2 , Wagaba Henry 1 , Titus Alicai 1 , Yona Baguma 1 , SeLumba B. Mukasa 2 , Anton Bua 1 and Nigel Taylor 3 Cassava (Manihot esculenta) in Uganda is faced with bio5c and abio5c stresses. Gene5c transforma5on has shown great poten5al in cassava gene5c improvement using 5ssue culture techniques like soma5c embryogenesis and organogenesis. The study focused on produc5on of, and regenera5on of friable embryogenic callus (FEC) and apical shoot 5ps (AST) . Fig. 1: Six month produc’on and regenera’on of FEC. A) leaf lobe, B) Organised embryogenic callus (OES), C) FEC, D) Germina5ng FEC, E) Mature cotyledon embryo, F) Germina5ng cotyledon embryo and G) Fully regenerated plant. Three genotypes tested. Fig. 2: Three month regenera’on process of ASTs. A) Sterilized AST, B) Excised AST, C) Germinated AST, D) Leaves emerging from a germinated AST and E) Regenerated plant. Seven genotypes tested. 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 Aladu Ebwanatereka 60444 Surface area coverage of FEC produced (cm 2 ) Cassava genotypes Tyrosine Tryptophan No amino acid Amino acids 0 20 40 60 80 100 120 Nase 3 Nase 12 Nase 13 Nase 14 Nam 130 Tme 204 Bukalasa Percentage regenera’on from Apical Shoot ’ps (%) Cassava Cul’vars BAP levels 1 BAP 0.5 BAP Fig. 3: Mean produc5on of FEC in media supplemented with amino acids Apio et al., 2015, A. J. B. Vol. 14(22), pp. 18541864 Tyrosine and BAP respec5vely facilitated produc5on of FEC and regenera5on of ASTs. A B C D E F G A B C D E Results Materials and Methods Introduc’on Conclusion 1 National Crops Resources Research Institute, Kampala, Uganda. 2 College of Agricultural and Natural Sciences, Makerere University, Kampala, Uganda. 3 Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO, 63132, United States. Fig. 4: Effect of cytokinin on regenera5on of ASTs of cassava genotypes World Congress on Root and Tuber Crops, Nanning, Guangxi, China, January 18-22, 2016
Transcript of SeLumba)B.)Mukasa2,)Anton)Bua1andNigelTaylor 3 ·...
Produc'on of and Regenera'on of Friable Embryogenic Callus and Apical Shoot Tips in Ugandan Farmer Preferred Cassava Genotypes
SP09-‐01
Hellen. B. Apio1,2, Wagaba Henry1, Titus Alicai1, Yona Baguma1, SeLumba B. Mukasa2, Anton Bua1 and Nigel Taylor3
Cassava (Manihot esculenta) in Uganda is faced with bio5c and abio5c stresses. Gene5c transforma5on has shown great poten5al in cassava gene5c improvement using 5ssue culture techniques like soma5c embryogenesis and organogenesis. The study focused on produc5on of, and regenera5on of friable embryogenic callus (FEC) and apical shoot 5ps (AST) .
Fig. 1: Six month produc'on and regenera'on of FEC. A) leaf lobe, B) Organised embryogenic callus (OES), C) FEC, D) Germina5ng FEC, E) Mature cotyledon embryo, F) Germina5ng cotyledon embryo and G) Fully regenerated plant. Three genotypes tested.
Fig. 2: Three month regenera'on process of ASTs. A) Sterilized AST, B) Excised AST, C) Germinated AST, D) Leaves emerging from a germinated AST and E) Regenerated plant. Seven genotypes tested.
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
Aladu Ebwanatereka 60444
Surface area coverage of FEC
produ
ced (cm
2 )
Cassava genotypes
Tyrosine Tryptophan No amino acid
Amino acids
0
20
40
60
80
100
120
Nase 3 Nase 12 Nase 13 Nase 14 Nam 130 Tme 204 Bukalasa Percen
tage re
gene
ra'o
n from
Apical Sho
ot 'ps (%)
Cassava Cul'vars
BAP levels
1 BAP 0.5 BAP
Fig. 3: Mean produc5on of FEC in media supplemented with amino acids
Apio et al., 2015, A. J. B. Vol. 14(22), pp. 1854-‐1864
Tyrosine and BAP respec5vely facilitated produc5on of FEC and regenera5on of ASTs.
A B C D
E F G
A B C
D E
Results
Materials and Methods
Introduc'on
Conclusion
1National Crops Resources Research Institute, Kampala, Uganda. 2College of Agricultural and Natural Sciences, Makerere University, Kampala, Uganda.
3Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO, 63132, United States.
Fig. 4: Effect of cytokinin on regenera5on of ASTs of cassava genotypes
World Congress on Root and Tuber Crops, Nanning, Guangxi, China, January 18-22, 2016
