Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No...
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Transcript of Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No...
![Page 1: Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.](https://reader035.fdocuments.us/reader035/viewer/2022070411/56649f555503460f94c78d49/html5/thumbnails/1.jpg)
Selective and Differential Media
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EMBSelective for Gram negative
E.coli has green metallic sheen
No growth of Gram pos Staph aureus
![Page 3: Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.](https://reader035.fdocuments.us/reader035/viewer/2022070411/56649f555503460f94c78d49/html5/thumbnails/3.jpg)
MSA: Selective and Differential
Selective because of salt: only Staph can grow
Differential because S. aureus ferments mannitol sugar (turns it yellow) but Staph epidermidis does not ferment mannitol. Note there is no growth or fermentation from M. luteus.
![Page 4: Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.](https://reader035.fdocuments.us/reader035/viewer/2022070411/56649f555503460f94c78d49/html5/thumbnails/4.jpg)
MSA with Staph aureus
![Page 5: Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.](https://reader035.fdocuments.us/reader035/viewer/2022070411/56649f555503460f94c78d49/html5/thumbnails/5.jpg)
Mitis Salivarius Agar
![Page 6: Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.](https://reader035.fdocuments.us/reader035/viewer/2022070411/56649f555503460f94c78d49/html5/thumbnails/6.jpg)
Mitis Salivarius
Agar
![Page 7: Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.](https://reader035.fdocuments.us/reader035/viewer/2022070411/56649f555503460f94c78d49/html5/thumbnails/7.jpg)
Mitis Salivarius Agar
![Page 8: Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.](https://reader035.fdocuments.us/reader035/viewer/2022070411/56649f555503460f94c78d49/html5/thumbnails/8.jpg)
BLOOD AGAR: Enrichment media, and Differential
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Fish Eye Colonies of
Enterobacter on blood agar
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MacConkey’s Agar:
Selective and Differential
Selective because Gram pos are inhibited by the bile salts. Differential because it has lactose. Gram negs that ferment lactose turn yellow.
![Page 11: Selective and Differential Media. EMB Selective for Gram negative E.coli has green metallic sheen No growth of Gram pos Staph aureus.](https://reader035.fdocuments.us/reader035/viewer/2022070411/56649f555503460f94c78d49/html5/thumbnails/11.jpg)
Antibiotic Sensitivity
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Antibiotic Sensitivity
Organism is resistant to the antibiotic
Organism is susceptible to the antibiotic
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To make 5 quadrants, draw a Y and then add 2 more lines like a mustache.
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Antibiotic Sensitivity: Check zone diameters on given table
Organism is resistant to the antibiotic
Organism is susceptible to the antibiotic
Organism has intermediate resistance to the antibiotic
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Coagulase Test:Differentiates S. Aureus from S epidermidis
Positive:S. aureus
Negative:S. epidermidis