Selected Antibody for Influenza Virus · WB analysis on MOCK ceillysates infected with different...

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Selected Antibody for Influenza Virus

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Influenza A viruses belong to the family Orfhomyxoviridae and have a segmented single-strand negative sense RNA genome (1). They represent one of the three types of influenza virus that infect humans, the other two being Band C. While influenza C is less common and generally milder clinically, influenza B viruses are increasingly being recognized as major contributors to the burden of influenza worldwide (2). Nevertheless, more of the research community's effort has been directed at influenza A, in part because these viruses are capable of causing potentially catastrophic pandemics through both mutation (antigenic drift) and genomic shuffling (antigenic shift).

Data suggests that only two subtypes of influenza A have been co-circulating recently. However, there are presently 17 recognized subtypes of influenza A (lurking in animals), in contrast to only two identified lineages of influenza B. These subtypes are based on the hemagglutinin (HA) glycoprotein, a trimeric molecule situated on the viral surface that binds to sialic acid residues on the host cell and mediates viral entry (3). HA is the primary consideration of influenza vaccine developers who strive to anticipate what form of influenza A will be the next year's primary threat.

GeneTex offers an outstanding selection of antibodies that cover a wide spectrum of influenza research, including reagents that detect antigenic determinants or other viral proteins. To explore the entire catalog of influenza antibodies please visit our website at www.genetex.com

Reference (1) Annu Rev Med. 2013;64:189-202. (2) Am J Public Health . 2013 Mar;1 03(3):e43-51 . (3) Adv Exp Med BioI. 2013;790:72-82.

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Influenza A

Monoclonal Anbibody C!l1lH!l

GTX80757

GTX28262

GTX40999

GTX40268

GTX40266

GTX40258

GTX40256

GTX40253

GTX41227

GTX41203

GTX41137

GTX41127

GTX41071

GTX41057

GTX41056

GTX40259

GTX629750

GTX629746

GTX629696

GTX629633

GTX28261

GTX629544

GTX76107

AlHA (GTX28262)

I: ••

Influenza A antibody [1331]

Influenza A HA antibody [C102]

Influenza A HA antibody [IVC102]

Influenza A H1 antigen antibody [58AB7-19-18]

Influenza A H1 antigen antibody [58CE8-1-5]

Influenza A H3 antigen antibody [30-2F11-F7-A5]

Influenza A H5 antigen antibody [11A9]

Influenza A H5 antigen antibody [15A6]

Influenza A H1 HA antibody [B213M]







Influenza A H1N1 HA antibody [C102 (IV.C102)]

Influenza A H1 N1 HA antibody [GT223]

Influenza A H1 N1 HA antibody [GT521]

Influenza A H1 N1 NA antibody [GT288]

Influenza A NP antibody [GT1236]

Influenza A NP antibody [IV F8]

Influenza A NP antibody [GT778]

Influenza A matrix protein antibody [GA2B]

KDa 130- A B

100 -70 -

55 -

40-

35 -

25-

AlHINI NA (GTX629696) IHe analysis using Influenza A hemagglutinin antibody IC 1 02).

W8 analysis on DF1 cell Iysates by using Influenza A Virus H 1 N 1 NA antibody IGT288) (GTX629696). Cells were either (A) non-infected or (8) infected with influenza A virus.

Polyclonal Antibody (jjJ1l& . ..

GTX40734 Influenza A antibody

GTX40841 Influenza A antibody

GTX36594 Influenza A H1 N1 HA antibody

GTX30852 Influenza A H1 N1 NP antibody



GTX125974 Influenza A H1 N1 NA antibody

GTX127984 Influenza A H5N1 NA antibody

GTX127303 Influenza A H7N7 HA antibody

GTX127305 Influenza A H9N2 HA antibody

GTX125990 Influenza A NS1 antibody

GTX125953 Influenza A NS2 antibody

GTX125923 Influenza A PB1 antibody

GTX125926 Influenza A PB2 antibody

GTX40910 Influenza A M1 protein antibody

GTX125928 Influenza A M1 antibody

GTX125951 Influenza A M2 antibody

GTX125989 Influenza A NP antibody

GTX125932 Influenza A PA antibody

I.r.J."t.; . . Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

KDa 170- A B

130 -

100 -

70 -

55 -

40 -

A/N P IGT7781 (GTX629S44)

''I

W8 analysis on DF1 cell Iysates by using Influenza A Virus NP antibody IGT7781 (GTX629544). Cells were either (A) noninfected or (8) infected with influenza A virus.

~ • GtpAb

GtpAb

GtpAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

GtpAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

I:. •• • ELISA, ICCIIF, IHC

ELISA, ICCIIF, WB

ELISA, FACS, ICCIIF, WB

ICC/IF

ICC/IF

ICC/IF

ELISA

ELISA

ELISA, WB

ELISA, WB

ELISA, WB

ELISA, WB

ELISA, WB

HI

ELISA

ELISA, ICCIIF, IHC-Fr

WB

WB

WB

WB

ELISA, ICCIIF

WB

ICC/IF, WB

KDa 170- A B

130 -

95-

72-

55-

43 -

AlNP IGTl2361 GTX629633 W8 analysis on DF1 cell Iysates by using Influenza A Virus NP antibody IGT1236) (GTX629633). Cells were either (A) noninfected or (8) infected with influenza A virus.

It •• • ELISA, ICC/IF , IHC, WB

Conjugation , ELISA, ICC/IF, IHC-P

ICC/IF, IHC-P, HI

ELISA, WB

WB

ICC/IF, WB

WB

WB

WB

WB

ICC/IF, WB

WB

ICC/IF, WB

ICC/IF, WB

Conjugation , ELISA, ICC/IF

WB

ICC/IF, WB

ICC/IF, WB

ICC/IF, WB

Avian Human

H1 H3 H5 H7 H9 H1 H1 H3 170_

1 ~~= ~;= . • 43-34-

25-

12345678

AIHINI HA (GTXI27295)

l

J

Avian Human

H1 H3 H5 H7 H9 H1 H1 H3

17~_ 1~5= 72 -55- _

43 -

34-

25 -

12345678

AI H5N3 HA (GTXI27299)

l J

170 -130-95-72 -55-43-

34 -

25-

Avian Human

H1 H3 H5 H7 H9 H1 H1 H3

1 2345678


l

J

170 _ 130 -95-72 -55-43 -

34 -

25 -

Avian Human

H1 H3 H5 H7 H9 H1 H1 H3

-12345678


WB analysis on MOCK ceillysates infected with different subtypes of human and avian influenza virus A by using influenza A Virus H1 N1 HA antibody (GTX127295), influenza A Virus H5N3 HA antibody (GTX127299) , influenza A virus H7N7 HA antibody (GTX127303), and influenza A virus H9N2 HA antibody (GTX127305) .

Influenza B

Monoclonal Anbibody ~~

GTX80759

GTX40245

GTX28260

GTX36912

GTX629882

GTX629883

GTX80383

GTX41022

GTX76108

GTX41044

GTX40925

KDa 130- A

100 -70-

55-

40-

35-

25-

. .. Influenza B antibody [1131]

Influenza B antibody [2/3 (3H4)]

Influenza B antibody [2/3]

Influenza B NP antibody [1181]

Influenza B NP antibody [GT371]

Influenza B NP antibody [GT382]

Influenza B NP antibody [B017 (B35G)]

Influenza B NP antibody [B272M]

Influenza B NP antibody [B017]

Influenza B M1 antibody [B276M]

Influenza B NA antibody [603]

B/NP IGT37 11 (GTX629882) B/NP IGT3711 (GTX629882) WB analysis on MDCK cell Iysates. Cells were either (A) non-infected or (B) infected with influenza B virus (Taiwan 70555).

Polyclonal Antibody fmiHil. ~ ..

GTX40533 Influenza B antibody

IF analysis on MOCK cells infected with influenza B virus by using influenza B virus NP antibody [GT371] (GTX629882; green).

GTX36591 Influenza B virions antibody

GTX128542

GTX128543

GTX128536

GTX128538

GTX128539

B/N P (GTXI28538)

Influenza B HA antibody

Influenza B HA antibody

Influenza B M antibody

Influenza B NP antibody

Influenza B NP antibody

KDa 170- A

130 -

100-

70-

55-

40-

B/HA (GTXI28542) IF analysis on MOCK cells infected with infiuenza B virus by using Infiuenza B Virus NP antibody (GTX128538; green) .

WB analysis on conditional medium by using Influenza B Virus HA antibody (GTX128542). (A) control. (B) viruscontained medium.

ffl;r.M • Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

Ms mAb

B/N P IGT3821 (GTX629883) IF analysis on MOCK cells infected with influenza B virus by using influenza B virus NP antibody [GT382] (GTX629883; green).

~ • GtpAb

GtpAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

Rb pAb

KDa 70- A

55-

40-

35-

25-

15-10 -

B/M (GTX I 28536) WB analysis on conditional medium by using Influenza B Virus HA antibody (GTX128536) . (A) control. (B) viruscontained medium.

L!. •• • ICCIIF

ELISA, ICC/IF, IHC-Fr, WB

I-ELISA, ICCIIF, WB

ELISA, ICC/IF, LFI

ICCIIF, WB

ICCIIF, WB

ELISA, ICC/IF, WB

ELISA, WB

ELISA, ICC/IF, WB, Functional Assay

ELISA, WB

2.500

2000

-+- Influenza B __ Influenza A

0500

o.oooo),.,---!:=¥:!::::~lO:=-=;:'OO~=;:;'ooo;=~,oooo MAb concentration(ng/mO

B/N P IB272MI (GTX41022) Titration curve of infiuenza B NP antibody [B272M] (GTX41022) in indirect ELISA.

I! r; jJ mBl1ffiI

ELISA, ICC/IF, WB

ELISA, ICC/IF, WB

WB

WB

WB

ICCIIF, WB

WB

~ A B 170-

130-

100-

70-

55-

B/N P (GTXI28539) WB analysis on conditional medium by using Infiuenza B Virus HA antibody (GTX128539). (A) control. (B) viruscontained medium.

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Conjugated antibody

Monoclonal Anbibody ...

Infiuenza A NP antibody [1331] (FITC)

Polyclonal Antibody ~~ l:fl •• ~l"I'llr:"url

GTX19997 Influenza A antibody (Biotin) GtpAb

GTX40030 Influenza A antibody (FITC ) GtpAb

GTX40388 Influenza A antibody (FITC ) GtpAb

GTX40534 Influenza A antibody (FITC) GtpAb

GTX36366 Influenza A H1N1 HA antibody (FITC) GtpAb

GTX36365 Influenza A H3N2 HA antibody (FITC) GtpAb

GTX36364 Influenza A matrix protein antibody (FITC) GtpAb

GTX36439 Influenza B antibody (Biotin) GtpAb

GTX36363 Influenza B antibody (FITC) GtpAb

GTX36515 Influenza B antibody (HRP) GtpAb

Avian influenza

Monoclonal Anbibody @iBtll!l. . .. r:mwl •

GTX41104 Avian Influenza A H5 HA antibody [B262M] Ms mAb

GTX41081 Avian Influenza A H5 HA antibody [B519M] Ms mAb

GTX41237 Avian Infiuenza A H5N1 HA antibody [B200M] Ms mAb

GTX41229 Avian Influenza A H5N1 HA antibody [B205M] Ms mAb

Polyclonal Antibody fmtllil. r:« •• ~"l."r:I.JJJ1

GTX41292 Avian Infiuenza A HA antibody Rb pAb

GTX41297 Avian Infiuenza A HA antibody Rb pAb

GTX127299 Avian Infiuenza A H5N3 HA antibody Rb pAb

GTX41304 Avian Infiuenza A NA antibody Rb pAb

GTX41305 Avian Infiuenza A NA antibody Rb pAb

Avian

50 - H t H3 H5 H7 H9

HAt n~ = 95 -37- 72-

55- -43 -

29 - 34 -

25-

AINP 113311 (GTX36902) AIHA (GTX41297) AlH5N3 HA (GTXI27299) IF analysis on cells infected with influenza A virus by using influenza A virus NP antibody 113311 (FITC) (GTX36902; green) .

WB analysis of HA 1 recombinant protein by using avian Influenza A HA antibody (GTX41297). (A) 5ng . (B) 25ng.

WB analysis on MOCK ceillysates infected with different subtypes of avian influenza A viruses by using avian Influenza A Virus H5N3 HA antibody (GTX127299) .

\

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' .. ELISA, ICC/IF, IHC

I~ •• '111

ELISA, ICC/IF

ICC/IF

ICC/lF, IHC-P

ICC/IF

ICC/lF, IHC-P, HI

ICC/lF, HI

ICC/IF

ELISA, ICC/IF, WB

ELISA, ICC/IF, WB

ELISA, ICC/IF, WB

,/~ .. • ELISA

Dot, ELISA, HI

ELISA

ELISA

[,:.,. ·l11f1'.IU·lll

ELISA

ELISA, WB

WB

ELISA

ELISA

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Selected Antibody for Influenza Virus · WB analysis on MOCK ceillysates infected with different...

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