SelectAZyme
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API Services & Chemical Development www.almacgroup.com Your Enzyme Choice Key Benefits: > Off the shelf biocatalyst kits > Enzyme screening, process optimisation and scale-up > Chiral building blocks > Metabolite synthesis > Dedicated biocatalysis group for seamless integration of technology > Rapid enzyme optimisation, evolution and supply > Simple business model / No IP roadblocks > mg to tonne manufacture
Transcript of SelectAZyme
API Services &Chemical Developmentwww.almacgroup.com
Your Enzyme Choice
Key Benefits:> Off the shelf biocatalyst kits
> Enzyme screening, process optimisation and scale-up
> Chiral building blocks
> Metabolite synthesis
> Dedicated biocatalysis group for seamless integration of technology
> Rapid enzyme optimisation, evolution and supply
> Simple business model / No IP roadblocks
> mg to tonne manufacture
Biom
arkerDiscovery&DevelopmentAPIServices&Chem
icalDevelopmentPharmaceuticalDevelopmentAnalyticalServicesClinicalTrialSupplyClinicalTechnologiesCommercialServices
for Synthesis: Metabolite Identification and Synthesis The selectAZymeTM platform is used for rapid metabolite synthesis, isolation, and structural identification.
> Chiral Synthesis> Bioresolution> Impurity Removal> Enzyme Kits
> Scale-up> Med-Chem Scaffolds > Manufacture> Bulk Enzyme Supply
MetaboliteSynthesis> Cloned Human P450
> Cloned Microbial P450
> Extensive microbial collection
MetaboliteIsolation/StructureID> Analytical and prep LC> LC/MS> High-field NMR
Case Study: Carbonyl Reductase to High ee Alcohol
Your A-Z enzyme selection for:
Case Study: Rapid Structural Identification
Case Study: Hydrolase Resolution of a ‘Difficult’ Alcohol
> High (>99%) ee achieved> Process scale to 100s kgs> Enzyme evolved to accept iso-propylalcohol
> mgs prepared> Preparative LC separation> Structure ID by LC-MS and NMR
> High (>99%) ee achieved> Simple extractive separation> Enzyme immobilised
All chemistry routes failed. selectAZymeTM used for production of a key hydroxylated metabolite needed for further studies. Potential for further process optimisation and scale-up to multi-kilogram scale or even commercial production.
Numerous attempts by others have failed to define a chemical process to provide 99% ee product. Chemocatalysis, classical resolution and enzyme resolution all failed.
Customer detected two metabolites at >10% of total drug load by HPLC. Almac selected the best biocatalyst for production of each metabolite from its enzyme and microbe collections.
EnzymeSelection 100s g
2-3wks 1-2wks 3-5wks 2-3mths 6-8mths
1-10 Kg 100 Kg Tons
Classical salt resolution process: Low yield, slow filtration, not scalable. selectAZymeTM process defined in two weeks: High yield, simple separation.
Case Study: Metabolite Synthesis and Scale-Up
> Microbial conversion carried out at 500 litre scale
> 100 g of desired metabolite produced in 3 weeks
> Process is scalable to kilogram scale as needed
Metabolite B
Metabolite AAPI
Biom
arkerDiscovery&DevelopmentAPIServices&Chem
icalDevelopmentPharmaceuticalDevelopmentAnalyticalServicesClinicalTrialSupplyClinicalTechnologiesCommercialServices
For European Enquiries:AlmacSciencesAlmac House20 Seagoe Industrial Estate, Craigavon, BT63 5QD, United Kingdom.T: +44 (0) 28 3833 2200E: [email protected]
For American Enquiries:Almac Group Incorporated25 Fretz RoadSouderton, PA 18964United States of AmericaT +1 (215) 660 8500 E: [email protected]
www.almacgroup.com
Almac Biocatalysis Summary:
Contacts: Dr.TomMoodyHeadofBiocatalysisT:+44(0)2838365906E:[email protected]
Dr.MichaelCannarsaT:+1(215)6107557621E:[email protected]
AlmacDavidKeirBuildingStranmillisRoadBelfastBT95AGUK
