Editorial BoardHEM Kay }(Joint Editors)G Slavin jRAB DruryGL GibsonNR GristLA Hanson (Sweden)Eadie HeydermanMSR HuttWJ JenkinsDM JonesEJL LowburyB Naylor (USA)MG RinslerJ StuartC Sultan (France)

A VollerJT WhicherHB Goodall (PublicationsSecretary, Association of ClinicalPathologists)Joan F Zilva (representing theAssociation of ClinicalBiochemists)AI Spriggs (representing theBritish Society of ClinicalCytology)ICM Maclennan (representingthe British Society forImmunology)EDITOR British Medical JournalBeryl Flitton (Technical Editor)

General Advisory BoardEN AllottRosemary BiggsSir Frank M Burnet (Australia)Sir John Dacie, FRSWM DavidsonM HynesNH MartinDorothy S RussellE Joan StokesMM Wintrobe (USA)

Officers of the Association of Clinical PathologistsPresident: Sir Robert Williams Chairman of Council: AC HuntVice-Presidents: I Rannie Honorary Secretary: GW Pennington

CAK Bird Honorary Treasurer: J BurstonPresident-Elect: WD Linsell Meetings Secretary: Rosalinde Hurley

Papers should be sent in duplicate to the Editors, Journalof Clinical Pathology, BMA House, Tavistock Square,London WC1H 9JR. They should be in double-spacedtypewriting on one side of the paper only. The name ofthe author with initials (or distinguishing Christian name)should be followed by the name and address of the lab-oratory where the work was performed. A definition ofthe position held by each author should be stated in acovering letter to the Editor. All papers with jointauthorship should be accompanied by the signatures ofall the authors. Results should not be shown as both tablesand graphs.ACKNOWLEDGMENT A stamped addressed postcard shouldbe enclosed for return to the author as acknowledgmentof receipt of the manuscript. Overseas authors shouldenclose international reply paid coupons.ILLUSTRATIONS Diagrams should be drawn in Indianink on stout white paper. The legends for illustrationsshould be typed on a separate sheet. Photographs andphotomicrographs should be on glossy paper, unmounted.CHEMICAL FORMULAE should as far as possible be writtenin a single horizontal line.ABBREVIATIONS In general, symbols and abbreviationsshould be those used by the Biochemical Journal.Si UNITS The units in which the work was done shouldbe cited first followed by traditional units or SI unitsin parentheses. A conversion factor shall be given as afootnote to tables and in legends to figures. Such con-version is the responsibility of the author.EmIcs The critical assessment of papers submittedwill include ethical considerations.REFERENCES Number references consecutively in theorder in which they are first mentioned in the text. Identifyreferences in the text by arabic numerals (in parentheses).References cited only in tables or in legends to figuresshould be numbered in accordance with a sequenceestablished by the first identification in the text of the par-ticular table or illustration. The references should begiven in the form used by Index Medicus:Journal (List all authors when six or less; when seven ormore, list only first three and add et al.)Soter NA, Wassermann SI, Austen KF. Cold urticaria:

release into the circulation of histamine and eosinophilchemotactic factor ofanaphylaxis during cold challenge.N Engl J Med 1976;294:687-90.

BookOsler AG. Complement: mechanisms and functions.Englewood Cliffs: Prentice Hall, 1976.

It is the responsibility of the author to ensure that references are correctly quoted.REPRINTS Twenty-five reprints will be supplied free ofcharge and further reprints may be ordered when theproof is returned.NOTICE TO ADVERTISERS Applications for advertisementspace and for rates should be addressed to the Advertise.ment Manager, Journal of Clinical Pathology, BMAHouse, Tavistock Square, London WC1H 9JR.NOTICE TO SUBSCRIBERS The Journal ofClinical Pathologyis published monthly. From 1 January 1981 the annualsubscription rates will be £40 in the UK and the Repub.lic of Ireland and US$95.00 in all countries overseas.Payment for overseas subscriptions should be made inUS dollars, ie, US$95.00, or in your national currencybased on the prevailing exchange rate to the US dollarof that currency, payable to the British Medical Assoc-iation. Orders can also be placed locally with any leadingsubscription agent or bookseller. (For the convenience ofreaders in the USA subscription orders, with or withoutpayment, can also be sent to British Medical Journal,1172 Commonwealth Avenue, Boston, Mass 02134. Allenquiries, however, must be addressed to the publisherin London.) All enquiries regarding air mail rates andsingle copies already published should be addressed tothe publisher in London.COPYRIGHT ©) 1980 Journal of Clinical Pathology. Thispublication is copyright under the Berne Convention andthe International Copyright Convention. All rights reser.ved. Apart from any relaxations permitted under nationalcopyright laws, no part of this publication may be repro.duced, stored in a retrieval system or transmitted in anyform or by any means without the prior permission of thecopyright owners. Permission is not, however, required tocopy abstracts of papers or of articles on condition that afull reference to the source is shown. Multiple copying ofthe contents of the publication without permission isalways illegal.

Heparin stability: effects of diluent, heparin activity, container, andpH 1211

References

Stock SL, Warner N. Heparin in acid solution. Br Med J1971 ;3:307.

2 Hodby ED, Hirsh J, Adeniyi-Jones C. The influence ofdrugs upon the anticoagulant activity of heparin. CanMed Assoc J 1972;106:562-4.

3 Mitchell JF, Barger RC, Cantwell L. Heparin stability in5% dextrose and 0-9 % sodium chloride solutions. Am JHosp Pharm 1976;33:540-2.

4 Joy RT, Hyneck ML, Bernardi RR, Ho NFH. Effect ofpHon the stability of heparin in 5 % dextrose solution. Am JHosp Pharm 1979;36:618-21.

Jacobs J, Kletter D, Superstine E, Hill KR, Lynn B, WebbRA. Intravenous infusions of heparin and penicillins.

J Clin Pathol 1973;26:742-6.6 Okuno T, Nelson CA. Anticoagulant activity of heparin in

intravenous fluids. J Clin Pathol 1975;28:494-7.7 Yin ET, Wessler S, Butler JV. Plasma heparin: a unique,

practical submicrogram-sensitive assay. J Lab Clin Med1973 ;81 :298-310.

8 Refn 1, Vestergaard L. The titration of heparin with prota-mine. Scand J Clin Lab Invest 1954;6:284-7.

Chessels JM, Braithwaite TA, Chamberlain DA. Dextroseand sorbitol as diluent for continuous intravenousheparin infusion. Br MedJ 1972;2:81-2.

Requests for reprints to: Dr AS Gallus, Department ofHaematology, Flinders Medical Centre, Bedford Park,South Australia 5042.

Reports and Bulletins prepared by the Association of Clinical BiochemistsThe following reports and bulletins are published by the Association of Clinical Biochemists. They may be obtainedfrom The Publishing Department, British Medical Journal (ACB Technical Bulletins), BMA House, TavistockSquare, London WC1H 9JR. Overseas readers should remit bv British Postal or Money Order.

SCIENTIFIC REVIEWS (price £1 00/$2.00 each)

1 The assessment of thyroid function March 1971FV FLYNN and JR HOBBS

2 Renal function tests suitable for clinical practiceJanuary 1972 FL MITCHELL, N VEALL, and RWE WATITS

3 Biochemical tests for the assessment of fetoplacentalfunction May 1975 CE WILDE and RE OAKEY

4 Test of exocrine pancreatic function March 1977AH GOWENLOCK

5 Assay of cholinesterase in clinical chemistry March1979 ELSIE SILK, J KING, and MARY WHHTAKER

TECHNICAL BULLETINS (price £1-00/$2.00 each)

22 Bilirubin standards and the determination of bilirubinby manual and technicon AutoAnalyzer methodsJanuary 1971 BARBARA BILLING, RUTH HASLAM, and NWALD

23 Interchangeable cells for spectrophotometers andfluorimeters September 1971 SS BROWN and AHGOWENLOCK

24 Simple tests to detect poisons March 1972 BWMEADE et al.

25 Blood gas analysers May 1972 K DIXON

26 Kits for enzyme activity determination September1972 SB ROSALKI and D TARLOW

27 Assessment of pumps suitable for incorporation intoexisting continuous flow analytical systems November1972 A FLECK et al.

28 Routine clinical measurements of transferrin inhuman serum September 1973 K DIXON

29 Control materials for clinical biochemistry (5thedition) September 1973 JF STEVENS30 Notes on the quality of performance of serumcholesterol assays September 1973 ss BROWN

31 Determination of uric acid in blood and in urineJuly 1974 RWE WArrS

32 A survey of amino acid analysers readily available inthe United Kingdom September 1974 JE CARLYLEand P PURKISS

33 Definitions of some words and terms used in auto-mated analysis November 1974 A FLECK, R ROBINSON,SS BROWN, and JR HOBBS

34 Measurement of albumin in the sera of patientsJanuary 1975 LINDA SLATER, PM CARTER, and JR HOBBS

35 Investigation of the validity of temperature correctionfactors for serum aspartate and alanine transaminasesMarch 1975 SB ROSALKI et al.36 Factors influencing the assay of creatinine November1975 JGH COOK

37 A survey of enzyme reaction rate analysers readilyavailable in the United Kingdom July 1977 RASAUNDERS and RF BURNS

38 Transport of specimens for clinical chemistry an-alysis November 1977 P WILDING, JF ZILVA, andCE WILDE

39 A scheme for the evaluation of diagnostic kits May1978 PH LLOYD

40 A practical guide to gamma-counting in radio-immunoassay January 1980 CE WILDE and D OTTEWELL

41 The use of biochemical tests tin the diagnosis ofdisorders of calcium metabolism July 1980 ANGELAFAIRNI5Y

Uook reviews 1225

vears of their course. This book advocatesa selective approach to the requesting oftests, rather than the uncritical use ofbatteries of investigations. It is not alaboratory textbook of practical clinicalchemistry but rather a book that providesgeneral information about the biochemicalbasis of disease.

In this second edition several chaptershave been extensively revised, and a newchapter on clinical chemistry in geriatricshas been added. It seems a pity that thebatter is so limited in scope, occupyingonly six pages. Geriatric chemical pathol-ogy is likely to be a marked growing pointin the future and demands greater atten-tion. It is pleasing to note the attempts bythe authors to deal with such topics astherapeutic monitoring, computers, profileand screening methods of investigation,the terminology of SI units, and side roomchemical tests, in addition to chemicalinvestigation, but the growth of chemicalpathology is such that to encompass allof these items in a single textbook isbecoming extremely difficult.A very readable and comprehensive

book which can be recommended to allworking in a hospital laboratory.

GW PENNINGTON

Atlas ofHuman Hemopoietic Development.E Keleman, W Calvo, and TM Fliedner.(Pp 266;343 figs; 204 in colour; US$202.40.) Springer-Verlag. 1979.

The authors' aim is to demonstrate theessential histological, cytological, andelectron microscopic findings of variousstages of human haemopoietic develop-ment obtained during a study of 190embryos and fetuses.An introductory chapter discusses the

concept of 'first generation haemopoieticcells' in yolk sac, chorion, and body stalk,with subsequent stem cell migration viavitilline and umbilicus vessels to establishintraembryonic 'second generation'haemopoiesis in liver. Further stem cellmigration results in bone marrow andsplenic haemopoiesis, and lymphopoiesisin lymph node and thymus.The three photographic sections are

logically presented in chronological order;the first deals with extraembryonichaemopoiesis, the second with intravas-cular haemopoietic cells, and a finalsection with 'organ-bound' haemopoiesis.The photography is of high qualitythroughout. Black-and-white photographsare used mainly to illustrate organ

development, and there are excellentcolour photographs of haemopoieticcells. The electron microscopy includessome fascinating pictures of cellularcontacts between endodermal environ-mental cells and erythropoietic precursorsThe text however is not quite so satisfying.The section on embryology lacks illustra-tions and had me searching for anembryology text containing explanatoryline drawings. Furthermore although theauthors acknowledge the limitations ofmorphology in identifying stem cells thephotographic legends abound with 'assum-ed', 'presumed', and 'apparent' progenitorcells, perhaps thereby confirming theillusive nature of the human pluripotentstem cell. Despite these criticisms I thinkthe book would be a useful reference forthose working in this field and may alsoprovide some interesting insights for theamateur.

CA SIEFF

Cancer: Assessment and Monitoring. EdSir Thomas Symington, AE Williams, andJG McVie. (Pp 412; illustrated; £22.)Churchill Livingstone. 1980.

Publications on cancer and cancer-relatedtopics are appearing at a rate which,many feel, far exceeds the generation ofnew information. As a result, many itemsare widely dispersed in the literature, andselective reading becomes harder tomaintain. The very diversity of muchrelated information, extending from basicscience at one end of the spectrum toclinical trials at the other, does identifythe need for books that assemble factsconcerning associated topics under stricteditorial control.

This book contains a distillate of paperspresented at the Tenth Pfizer Symposiumon the theme of the assessment andmonitoring of cancer. An introductorychapter on the biology of cancer bySymington sets the scene for chapters onclinical assessment, pathological tech-niques and diagnosis, tumour immunology,radiological assessment, nuclear medicineand assessment, biochemical aspects, anda final section on topics related to datacollection. Although some chapters relateto information of little immediate valuein the current understanding of diseaseassessment, others are informative andwell referenced.The heterogenous quality of the

chapters reflects the blanket publicationof a symposium, but the critical reader

who has the time to browse through thisbook will find several bright spots whichwill amply justify his effort.

JMA WHITEHOUSE

A Colour Atlas of Tumour Histopathology.NFC Gowing. (Pp 212; illustrated;£35.) Wolfe Medical Publications.1980.

Nine hundred and forty-four colourphotographs of Royal Marsden Hospitalhistopathology specimens, with commentsby the professor, should be impressive,interesting, and instructive. It is all ofthese, even though the pictures are smalland the comments are brief.

It is no fault of the author if this bookdoes not prove to be of great importance.Histopathology is not just patternmatching, and atlases are not workingbench books. This atlas will be well usedby membership candidates in their periodof purgatory between the papers and thepractical, but many of us may hope thatthe author will have another opportunityof combining colour photographs with amore extensive text-colour histopathol-ogy in depth. Gardeners, ornithologists,and cooks are now well provided withcolour publications, and this book, one ofthe best of its kind, shows the way aheadfor histopathology and colour photo-graphy.

RAB DRURY

NoticeThe Casey Holter Memorial Prize

Entries are invited for the Casey HolterMemorial Prize, which will be awarded in1981 for the best essay reporting originalwork bearing on the pathogenesis ortreatment of spina bifida or hydroce-phalus. The prize will be £250. Entriesshould be submitted before 30 April1981 to Mr DG Young, Royal Hospitalfor Sick Children, Yorkhill, Glasgow,G3 8SJ, UK.

The members of the Editorial Board and the General Advisory Board are indebted to the following for their helpwith the assessment of papers during the year to 30 September 1980:

J Hume AdamsMK AlexanderJune D AlmeidaT AndersonHilary J AndrewsNH AshtonJG AzzopardiJ BallDN BaronCP BeattieJOW BeilbyAJ BellinghamMH BennettLucille BitenskyAJ BlackshawJN BlauAL BloomR BlowersJ BoydDJH BrockSS BrownM BrozovicW BrumfittJS CameronHM CameronM CasewellD CatovskyI ChanarinPJ CiclitiraYvonne M ClaytonJC ColemanEC ColesJG ColleeH CookB CorrinWAJ CraneT CrowRC CurranPM DanielJF DavidsonIMP DawsonD DenisonHeather M DickJ DobbingOG DodgeJean DolbyBS DrasarV DubowitzJA DudgeonDC DumondeMS DunnillRD EasthamJoan MB EdwardsJM EnglandRJ FallonTen FeiziDG FleckTH FlewettPT FluteFV FlynnH FoxAL FurnissDL GardnerH GayaAAM GibsonRR Gillies

A GoldbergAH GoldstoneHB GoodallEC Gordon-SmithNFC GowingAE GreenChandra GrubbJM GumpelPJ HamiltonRM HardistyMJ HealeyDK HenryR HimsworthJR HobbsAV HoffbrandEJ HolborowJB HoltonElizabeth HudsonHelen E HughesNC Hughes-JonesRosalinde HurleyOAN HusainPG IsaacsonA JacobsJR JassL KinlenJ KohnR KumarE LarsenDJR LaurenceFD LeeB LennoxEA LetskyA LevenePD LewisSM LewisJA LoraineJ LorberVR McCreadyGA McDonaldH MacDonald ClinkJ O'D McGeeJE MacIverAM MackayRNM MacSweenD McSwigganCR MadeleyV MarksPD MeersISI MenziesL MichaelsN MillerDA MitchisonDL MollinB MorsonAP MowatAM NevilleJR O'BrienFW O'GradyS PattersonDE PeggD PepperI PhillipsTAE Platts-MillsB PortmanJM Preece

AB PriceFJ PrimeRCB PughJH ReesJ RichmondDS RidleyCRC RizzaWB RobertsonFG RodgersKB RogersKL RogersS RosalkiGA RoseM RoseB RoweAJ SalsburyDiana SamsonP SandersonM SandlerPJ ScheuerA SegalP SelbyJB SelkonAA SharpElizabeth ShawC SieffMB SkirrowBrenda M SlavinJC SloperG SmithJL SmithDCE SpellerC SternA StuartSir Tom SymingtonAG TaylorCED TaylorD Taylor-RobinsonDP ThomasH ThompsonJR TigheAS ToddPG TonerCR TribePA TrottDR TurnerMargaret Turner-WarwickD TyrrellH ValdimarssonEA WallingtonEN WardleA WatersPamela M WaterworthRWE WattsJM WhiteAE WilkinsonPC WilkinsonAT WillisMLN WilloughbyCG WoodsN WoolfDH WrightMI WrightAJ Zuckerman

Volume 33: Author indexAbbreviations and symbols:--'(L)' refers to letters to theEditor; *indicates first-named author.

ABBOTT, J. D., 762*ABDALLA, S., 958ABELA, M., 796* (L)ACKERMAN, V. P., 830*ADAMS, D. M., 488ADAMS, J. H., 1132*ADDIS, M., 946ADINOLFI, M., 269AGARWAL, L. P., 1169AJINA, N., 24AKHTAR, M., 1214*ALDRED, M., 282ALEXANDER, T. A., 1169ALLEN, S C., 430*ALLEN-MERSH, T. G., 1212ANDRES, T., 1165*ARDEN, G. B., 327ARONSTAM, A., 438AROSIO, E., 183, 1013 (L)ARSTALL, S., 1095ASHRAF ALI, N., 1214ATKINS, N., 1010AUBREY, C. S., 1222* (L)AUDOUIN, J., 1068AXON, A. T. R., 604 (L)AZNAR. J., 602* (L), 973BAIN, B., 449*BAKRY, M., 1214BALFOUR, A. H., 644*BALFOUR, I. C., 430BALL, L. C., 47, 53BALSARI, A., 640*BALTZER, I. M., 408BARBARA, J., 1216*BARBENEL, J. C., 373BARBUI, T., 434BARNES, M. P., 481*BARNHAM, M., 314* (L)BARNICOAT, M., 744BARONE, C., 936BARR, A., 1106BARRETT, B. A., 1118*BARROWCLOUGH, H., 1064*BARSON, A. J., 679BARTOLINI, C., 936*BASCOMB. S., 36*BATCHELOR, J., 744BATESON, M. C., 131*BEARD, H. K., 1077*BELCH, J. J. F., 373BENJAMIN, J., 603 (L), 1122BERNADOU, A., 1068BERNEY, J., 917BERRY, C. L., 317*BESLEY, G. T. N., 336BHARUCHA, H., 81BIDWELL, D. E., 200* (L)BIDWELL, E., 954

BIEVA, C., 815BILSKI-PASQUIER, G., 1068BINI, A., 631BIRCH, C., 414* (L)BIRD, R., 528BISERTE, G., 823BITENSKY, L., 684BODDINGTON, M. M., 517BONATO, D., 183, 1013 (L)BONDI, A., 904* (L)BONIOLO, A., 640BORNEMISZA, A., 794BOUCHIER, I. A. D., 131BOWELL, P. J., 249, 954, 958*BOYD, J. F., 11BRADBURY, R., 830BRADFIELD, J. W. B., 365BRAVENY, I., 841BRETON-GORIUS, J., 1110BRIDGER, R. C., 276*BRIDGES, J. B., 644BRIGGS, J. C., 702* (L)BRITTON, J. A., 151, 194BROADBENT, E., 24*BROOK, I., 848, 1099*BROWN, I. R. F., 966*BROWNING, J. D., ll*, 890BROZOVIC, M., 1202BRYDEN, A. S., 413*BULL, H., 1202*BULL, T. B., 327BULMAN, A., 1219 (L)BURDON, D. W., 1012 (L)BURNS, J., 1021BURNSIDE, P., 730BURTON, M., 98 (L)BURTON-KEE, j., 653*BUSSOLATI, G., 904 (L)CAINE, S., 1220 (L)CANNON, D., 160CAO, A., 946, 949CARO, J. F., 868*CARTER, N. D., 966CARTER, N. W., 493*CASTRO, J. E., 990CAUDRON, M., 815*CAVE. R. H., 312*CEPIL, B. A., 585CHALMERS, D. M., 3*CHAN. S. H., 792*CHANARIN, I., 3, 909*CHANTLER, S. M., 200 (L)CHAYEN, J., 684CHILD, J. A., 757CHIN, Y., 684CHOOT, C.-C., 1206CLEAR, A. S., 544CLOWES, M., 145*

1227

CRAFT, A. W., 735*CRAWFORD, R. J., 180*, 1106CROKAERT, F., 1189CROW, j., 72*CROZIER, D., 390CODD, A. A., 191CODLING, B. W., 351COHEN, J. L., 377CONTRONI, G., 848COOK, C. B., 993COOKE, E. M., 188COREY. M., 390CORNACCHIA, G., 949CORNELISSE, C. J., 125CORNWELL, G. G., III, 1146*CORRIN, B., 891*CORTES, J. M., 899cossu, P., 949COTTIER, H., 454COWEN, P. N., 797* (L)COX, S. E. M., 386CUBITT, W. D., 306*, 1095*CUMMING, C. G., 691CURRAN, R. C., 1047*DALE, B. A. S., 762, 767DALEY, D., 508 (L)DANDONA, P., 1102DANE, D. S., 851DAROUGAR, S., 171DAVIDSON, D., 493DAVIDSON, G. P., 390*DAVIDSON, J. F.. 445DAVIDSON, R. J., 1193*DAVIES, D. R., 544DAVIES, E., 1082DAVIES, S. W., 1222* (L)DAVIES, T. D., 1216DAVIS, M. R., 562, 1223 (L)DAVIS, P., 648*DAWSON, D. W., 234*DAWSON, J. E., 702 (L)DELAMARE, N., 1189DELAMORE, I. W., 234DE LOUVOIS, j., 66*, 575*DERVAN, P., 160DE VIRGIIIIS, S., 946, 949*DEVLIN, J. D., 438DHALL, D. P., 562, 1223 (L)DHALL, T. z., 562, 1223 (L)DICKINSON, R. J., 604* (L)DIEBOLD, J., 1068*DIEDERICHSEN, H., 876*DIXON, A. J., 1021*DIXON, M. F., 604 (L)DJALDETTI, M., 136DONATI, M. B., 631DORAN, G., 1212*DORLING, J., 897*

1228 Author indexDOVIS, M., 640DOW. B. C., 1106*DOYLE, D., 1132DRESCH, C., 1110*DRUMMOND, M. M., 373*DUCKWORTH, T., 462DUGUID, H. L. D., 131DUNCAN, B. MCL., 413 (L)DUNCAN, L. j. P., 163DUNNILL, M. S., 1021EASMON, C. S. F., 386*EDLIN, G. P., 526*EDWARDS, M., 509 (L)EGGLESTONE, S. I., 798* (L)EGGLETON, C., 534ELDRIDGE, J., 762, 767ELION-GERRITZEN, W. E., 902* (L)ENGLAND, J. M., 905* (L)ENOMOTO, H., 786ERSKINE, J. G., 445*EUSEBI, V., 904 (L)EVANS, E. G. V., 750EVANS, P., 995EVANS, R. T., 591*FARGION, S., 949FARMER, T., 297FARRELL, M. A., 547FARRINGTON, C. L., 926FARTHING, M. J G., 344FEDELI, F., 904 (L)FEREMANS, W. W., 815FERGUSON, I. A., 562, 1223 (L)FERLITO, A., 1160FIORELLI, G., 949FIRTH, R., 1197FISH, D. i., 234FLAHERTY, T. A., 234FLAMINI, G., 936FLANDRIN, G., 1110FLECK, A., 1220* (L)FLEMANS, R. j., 738FOLLETT, E. A. C., 1017*FOO, Y., 1102*FORBES, C. D., 373FORSEY, T., 171*FORSTNER, G. G., 390FOULIS, A. K., 1125*FOX, B., 327*FRANKLIN, J. C., 93*FRASER, C. A. M., 47, 53FRASER, C. G., 903* (L)FREEDMAN, j., 977*FREEMAN, R., 28*, 770FRERICHS, G. N., 905* (L)GABER, M., 199 (L)GAD-AL-RAB, j., 660GAFTER, U., 136*GALANELLO, R., 946*GALLUS, A. S., 1206GAMBASSI, G., 936GAMBLE, D. R., 871GANE, P., 885GARDNER, M. D., 380*GARDNER, P. S., 735GEMMELL, C. G., 702 (L)GENTILONI, N., 936GEORGE, A. J., 622GHONEIM, A. T. M., 188*, 757GIBBONS, A. R., 1219 (L)GILBERT, R. j., 314 (L)

GILES, C., 145GLENNON, J. A., 868GLOVER, V., 963*GOFFIN, Y., 262*GOODALL, K. T., 1206*GORDON, H., 72GORDON, j., 539*GOURDIN, M. F., 609GOWENLOCK, A. H., 234GRAHAM, D. I., 1132GRAHAM, D. T., 562GRAINGER, J. M., 309GRANDIER-VAZEILLE, x., 823GRAY, R. S., 163GREAVES, P. W., 581*, 1184GREEN, A. E., 1114GREENBURY, C. L., 1077GREGOR, R. T., 705GREGORY, J., 1047GRIFFITHS, P. D., 493GRIMLEY EVANS, j., 905 (L)GRIST, N. R., 471*, 702* (L)GROOT OBBINK, D. j., 830GROULS, V., 552*GRUNDMANN, E., 711GRUNEBERG, R. N., 853*GUBASH, S. M., 395*GUNSON, H. H., 249*, 954, 958GUPTA, I., 679*GUST, I., 414 (L)HAENEY, M. R., 500HAMBLING, M. H., 28HAMILTON, J. C., 197*HAQQANI, M. T, 1058HARBOUR, j., 774*HARFORD, J. P., 644HARRIS, J. B., 1216HARVEIT, F., 700* (L)HAUSE, L., 864HAWKER, A., 414 (L)HAWKINS, 0. F., 895*HAWKINS, L., 779HEDLEY, J. M., 971*HEFTER, L. G., 993*HELIN, H., 95HENG, S. H., 792HERD, R., 163HESS, M. W., 454HEY, E. N., 534HEYDERMAN, E., 1219* (L)HEYSE-MOORE, G. H., 438HIGGINS, P. G., 774HIGGINSON, j., 779, 781HILDEBRAND, H. F., 823*HILL, P. I., 1118HIX, M. A., 993HLA-PE, U., 699*HLAVA, A., 984HOFFBRAND, A. V., 243, 963HOLLIDAY, M. G., 1174*HOLT, P. G., 1221 (L)HOLZEL, H., 306HONAN, R. P., 1058*HOPKINS, R., 19*HOPWOOD, D., 131HORNE, D. W., III, 134HOWARD, A., 386HOWARD, F. M., 400HOWELLS, C. H. L., 701* (L)HOWIE, A. J., 1012* (L)

HUA, A. S. P., 598HUBSCHMID, T., 454HUDSON, E., 72HUDSON, G., 697HUDSON, M. J., 1114*HUGHES, A., 963HUGHES, H. E., 811HUKINS, D. W. L., 995HUNT, L. P., 234HURLEY, R., 24, 575HUSAIN, 0. A. N., 309*, 684HUTT, M. S. R., 544HYDE, K., 234IBBOTSON, R. M., 145ICHORD, R., 848*INGOLDBY, C. J. H., 990*INTERNATIONAL COMMITTEE FOR STANDA)

IZATION IN HAEMATOLOGY, Expert Paon Blood Cell Sizing, 1*

IONS, V. M., 905 (L)ISICHEI, U. P., 626*ISLAM, A. K M. S., 1006*ITOH, Y., 786IVERSEN, 0. E., 688JAARSMA, K. W., 1064JACKSON, M. J., 86JAIN, R. K., 11lv9JAMES, D. G., 528JAMES, K., 163*JAMES, K. R., 899*JASS, J. R., 801 *JEWELL, D. P., 917, 921JOHANSEN, A., 711JOHNSON, A. P., 177JOHNSON, P. H., 145JONES, B. M., 598*JONES, D. M., 762, 767*, 1002JORDAN, T., 19KAHN. S. N., 617*KAIJSER, B., 408KAWAI, T., 786KENNY, M. W., 417, 622*KESSLER, E., 136KIN, K., 786KING, J. D., 297*KIRKWOOD, T. B. L., 249KNOWLES, M. A., 904 (L)KOHN, J., 617KOSTIALA, A. A. 1., 694*KRIVOSIC, 1., 823KUMAR, N. B., 1153*LABBE, M., 1189*LALJI, F., 1216LARSSON, P., 408*LATTIMER, G. L., 585*LAURENT, E., 815LAURENT, G., 609LAWLER, W., 1029*LAYCOCK, B. J., 151, 194*LEATHEM, A., 1010*LEBAS, P., 815LECHI, A., 183, 1013 (L)LEDINGHAM, 1. MCA., 890LEE, A., 830LEE, F. D., 811LEHNER, T., 269*LEINIKKI, P. o., 836LEONARD, R. C. F., 609LEVENE, A., 101*

Author index

LEVI, A. J., 3

LEWIS, M. J., 1184LEWIS, S. M., 243, 700* (L)LILLEYMAN, J. S., 151*, 194LINDEMAN, J., 125LITTLE, P. J., 58*LOON, A. M. VAN, 635*LOWE, G. D. o., 373LUCAS, D. F., 493LUCIE, N. P., 890*LUSCIETI, P., 454*LUTON, A., 744MCARDLE, B., 796 (L)MACARTNEY, J. C., 351*MCCANN, S. R., 1197*MCCLURE, J., 670*MACDERMOTT, S., 3, 254MCDONALD, G. S. A., 660*MCGEE, J. O'D., 1021MCINTYRE, R. L. E., 513*MACIVER, A. G., 1218* (L)MACIVER, J. E., 234MACKIF, I., 1202MCLELLAN, D. S., 438*MACPHERSON, B., 1165MCPHIE, J. L., 1193MCQUILLIN, J., 735MACRAE, A. D., 1184MCSWIGGAN, D. A., 1095MACVARISH, 1.. 1106MCWHINNEY, N. A., 990

MADIEDO, G., 864*MAHAJAN, V. M., 1169*MAKINEN, D., 648MALEY, A. D., 905 (L)MALLICK, N. P., 1029MALLON, R., 508 (L)MALMENDIERS, C. L., 815

MARKS, V, 871MARSDEN, H. B., 722MARSHALL, J., 414 (L)MARTIN. S., 757*MASON, D. Y., 609*MASSEY, A., 977MATTHEWS, J., 566

MEE, A. S., 917*, 921*MEIJER, C. J. L. M., 125

MELLING, J., 314* (L)MENGOLI, C., 183*, 1013* (L)MEPHAM, B. L., 1218 (L)MERA, S. L., 365*MERRIMAN, J., 163

MERRYWEATHER, M., 1220 (L)MEUTER, F. DE, 815

MICHAELS, L., 705*MIKHAILIDIS, D., 1102MILATOVIC, D., 841*MILLETT, J. A., 309, 684*MISHLER, J. M., 155*, 555*, 880*MITCHELL, R., 180, 1106

MOFFAT, A., 871*MOLINA, V., 640MOLLIN, D. L., 243*MONFORTE, M., 602 (L)MONTESI, G., 183, 1013 L)MORAD-HASSEL, F., 390)

MORE, 1. A. R., 11

MORGAN, M., 180MORGAN, M. Y., 488*MORGAN-CAPNER, P., 200 (L), 653, 1082*

MORRISON, G. D., 798 (L)MORSON, B. C., 711*MORTIMER, J. Y., 1120*MOWBRAY, J. F., 653MULHALL, A., 575MUNDAY. P. E., 177*MUNRO, R., 508* (L)MURGIA, v., 949MURRAY, J., 995*MURRAY, N., 1197MUSSONI, L., 631NAGAYO, T., 711NAGINGTON, J., 851*NARANG, H. K., 191*NARCISSI, P., 534NAYLOR, B., 1153NELSON, M. G., 926*NEVILLE, E., 528NEWMAN, P. F. J., 1009*NEWSOM, S. W. B., 566*NG, C. M., 488NICHOLLS, A. C., 534NICHOLS, T., 770*NICOLL, A., 691NIMMO, 1. A., 1009NOONE, P., 400NORRIS, P. D., 697

ODDS, F. C., 750*O DONNELL, J. R., 547*OLLING, S., 408ONN, C. J., 792OSUNA, J. j., 602 (L)OWEN, E., 1214OWOR, R., 1039PAGLIETTI, E., 946

PAIN, R. W., 413* (L)PANCERA, P., 183, 1013 (L)PAPADIMITRIOU, J. M., 1221 (L)PARADINAS, F. J., 899PARKER, L. S., 1132PARKHOUSE, E. J., 155, 880PARKIN, J. M., 534PARRY, P., 509 (L)PATON, K., 254PATRICK, W. J. A., 336*PATTISON, J. R., 200 (L), 1082PAYNE, C. M., 505*PAYNE, R. B., 413 (L)PEDDIE, B. A., 58

PENBERTHY, L. A., 903 (L)PEPERSACK, F., 1189PESAVENTO, G., 1160*

PETRUCCI, J. v., 905* (L)PETRUZZELLI, E., 640

PHILLIPS, P. j., 413 (L), 971PIRIS, j., 361*, 513PLOWMAN, P. N., 738*POLI, G., 640POMEROY, A. R., 562

POPE, F. M., 534*POWELL, D., 160POWLES, R. L., 344

PRITCHARD, R. C., 830

PURDY, G. W., 926PUSINERI, F., 631*PYLKKANEN, J., 694QURESHI, M., 796 (L)RAAM, S., 377*RADFORD, S., 781RAISANEN, S. M., 95*, 836*

RAMDIAL, L., 1102RANTALA, 1., 95READING, C., 297RECHER, G., 1160REES, T., 1092*REID, H., 722*REID, M. M., 735REMUZZI, G., 631REVILL, W. J., 900*REYNES, M., 1068REYNOLDS, K. W., 1212RIBEIRO, C. D., 509* (L)RICH, J. D., 134*RICHES, P. G., 617, 781RICKETTS, C. R., 155, 880RIDGWAY, G. L., 744*RIDLEY, D. S., 33RIISOM, K., 876RISER, E., 400*ROBERTSON, J. H., 730*ROBERTSON, K., 254RODEGHIERO, F., 434*RODGERS, F. G., 8*, 484RODGERS, F. S., 1184*ROGERS, J. D., 926ROGERS, K. B., 1086*ROLLASON, T. P., 351ROLLERI, E., 640ROSALKI, S. B., 604* (L), 1102ROSEKRANS, P. C. M., 125*ROSS, M. G. R., 488ROSS, P. W., 691*ROSS, S., 19ROUGER, P., 885*ROWBOTHAM, T. J., 1179*ROWE, B., 595RUGGERI, R., 946RUHEN, R. W., 1221* (L)RUSSO, M. A., 936RYVAR, R., 1077SALKER, R., 1216SALMON, C., 885SALMON, D. C., 730

SALONEN, E.-M., 845

SANDLER, M., 963SANNA, G., 949SANTOS, M. T., 973*SATTERFIELD, V. E., 505

SAWATZKE, C. L., 600*SCHMAUZ, R., 1039*SCIACCA, R., 864SCOTT, G., 1132SCOTT, R., 380SCURO, L. A., 183, 1013 (L)SEGADAL, E., 688*SELLS, P. G., 98* (L)SERCK-HANSSEN, A., 711

SHABTAY, F., 136SHAH, G. A., 562*SHAKED, P., 136SHALL, G. A., 1223* (L)SHANSON, D. C., 888*SHERLOCK, S., 488SHESOL, B. F., 134

SHINTON, N. K., 700 (L)SHIPP, A. P., 774SHIPP, C. R., 595*SHOUSHA, S., 199* (L)SHUTTLEWORTH, R., 1002*SIMMONS. N. A., 1222 (L)

1229

1230

SINCOCK, A. R., 58SKINGLE, J., 1077SKIRROW, M. B., 603* (L), 1122*SKRABANEK, P., 160*SLAVIN, G., 254*SLEIGH, J. D., 1017SLOANE, J. P., 344*SLOANE, N. G., 868SMIBERT, R. M., 509* (L)SMILLIE, D. M., 890SMITH, A. M., 167*, 500*SMITH, I I., 336SMITH, J. K., 954*SMITH, J. L., 539SNAPE, T. J., 958SOBIN, L. H., 454, 711SOKOL, R. j., 697*SOLOMONS, C. C., 600SONDHEIMER, J. M., 390SOOD, A., 966SOORAE, A. S., 81*SOWTER, C., 254SPENCER, R. C., 36SPIAZZI, G., 183, 1013 (L)SPRAGG, B. P., 509 (L)SPRIGGS, A. 1., 517*STARKEY, H., 200* (L)STEINER, 1., 984*STENKVIST, B., 700* (L)STICKLER, D. J., 288*STONER, M., 993STUART, J., 417*, 622STUDDY, P. R., 528*SUGDEN, P. J., 151

SUNI, J. I., 836, 845SUTCLIFFE, E. M., 762SUTHERLAND, R., 297

SUTOR. D. J., 86*SZAWATAKOWSKI, M., 921

TAKAGI, K., 786*

TAPPER-JONES, L., 282*TARPEY, P., 1029TAYLOR, M., 1002TAYLOR, R. J., 701 (L)TAYLOR-ROBINSON, D., 177, 205*. 591TEDDER, R. S., 851TEMPERLEY, i. J., 1197TERRANOVA, T., 936TETAERT, D., 823THOMAS, B., 288THOMAS, B. J., 177, 205THOMAS, E., 1006THOMAS, H. C., 488THOMAS, N. D., 361THOMPSON, R. A., 167, 500THORNTON, J. A., 234TIMPERLEY, W. R., 462

TIN-WIN, U., 699TOSSWILL, J. H. C., 33*TOZER, R.A., 544*TRICOT, G., 1068

TULLIEZ, M., 1068ULSTEIN, M., 688URBANIAK, S. J., 370VAANANEN, P., 845VAHERI, A., 845*VALLES, J., 973VAYA, A., 602 (L)VALLES, J., 973VEEN, J. VAN DER, 635VELLA, E. E., 1223* (L)VILCHES, J., 973VON GRAEVENITZ, A., 509 (L)WACHTEL, E., 990WAITKINS, S. A., 47*, 53*, 302*WAL, A. M. V. d., 125WALKER, B. E., 413* (L)WALKER, D. M., 282WALKER, F., 97WALKER, 1. D., 445

WALKER, R. A., 356*WALKER, S. M., 370*WALLER, D. K., 774WALLIS, M., 1222 (L)WANDLESS, I., 905* (L)WARD, J. D., 462WARD, P. G., 243WARDLE, E. N., 888*WARHURST, D. C., 33WATERS, H. M., 234WATERWORTH, P. M., 571*, 1092WATT, A. D., 19WEST, K. P., 97*WEST, P., 89*WESTERMARK, P., 1146WHICHER, J. T., 779*, 781*WHITBY, H. J., 484*WHITE, D., 314* (L)WIENER, K.. 857*WILKIE, L. I., 86WILKINSON, R. H., 900WILLIAMS, D. M., 555WILLIAMS, E., 462*WILLIAMS, G., 1029WILLIAMS, M., 702* (L)WILLIAMS, M. D., 794*WILSON, A., 798* (L)WISE, C. C., 430WOODINGS, D., 1202WREGHITT, T. G., 851WREN, M. W. D., 61*WRIGHT, E. P., 904* (L)YO, S. L., 792YOURASSOWSKY, E., 1189

YOUNG, E. W., 365YOUNG, J. A., 811*ZANEN, H. C., 474ZANEN-LINI, 0. G., 474*

Author index

Volume 33: Subject index

Abbreviations:-'(L)' refers to letters to the Editor.

Abscesses, liver, amoebic, counterimmunoelectrophoresis asscreening test for, 33-- of central nervous system, pus from, bacteriological

examination of, 66Acetylcholinesterase in rectal mucosal biopsies, comparison

of histochemical and biochemical activity of, and histo-chemical diagnosis of Hirschsprung's disease, 336

Acid phosphatase activity, tartrate-resistant, in paraffin-embedded tissue sections, diagnosis of hairy-cell leukaemiaby, 552

and sex in childhood non-T lymphoblasticleukaemia, 151

Acrylamide gel electropherograms, thin-layer, method forrecording of, 699

Adenoacanthoma of endometrium, separate entity orhistological variant of adenocarcinoma?, 1064

Adolescence, marrow hypoplasia with splenomegaly in, 730Aetiocholanolone and prednisolone, studies of alkaline

phosphatase as marker of neutrophil maturity in normalsubjects dosed with, 555

African children with sickle-cell disease, liver function anddiagnostic significance of biochemical changes in blood in,626

Agar dilution method, sensitivity tests performed by,evaluation of Replireader for recording of, 571

____ -_-sensitivity-testing, quality control by direct assayof antibiotic in solid medium, 93

Agarose electrophoresis, sensitivity for detection of BenceJones protein, comparison with immunofixation, 779

Age and site, lymph node morphology as function of, 454-, relation to histological appearance in condylomatous

tumours of vulva, vagina, and penis, 1039Age- and sex-related reference ranges for 8 plasma consti-

tuents derived from random adult population at Cumber-nauld, 380

Agglutination, grouping of 3-haemolytic streptococci by,386

Aging process, senile amyloidosis as protean manifestationof, 1146

Air bubbles and temperature, effect on blood gas analysis,864

Albumin concentrations, abnormal, calcium correction for,in myocardial infarction, variations between factors used?(L), 413

Alcohol consumption, excessive, haematological effects of,sex-related differences in, 3

Alcohol-related liver disease, HLA-B8, immunoglobulinsand antibody responses in, 488

Alcoholism, acute, cytoplasmic vacuolation of peripheralblood cells in, 1193

Alkaline phosphatase as marker of neutrophil maturity,studies in normal subjects dosed with aetiocholanolone andprednisolone, 555

isoenzyme, 'ultra-fast' (L), 604Allergy, food, diagnosis of, morphometry and immuno-

histochemistry of small intestinal biopsy specimens in,125

Alpha heavy chain disease, abnormal chromosomal marker(D14 q+) in, 136

Alpha-fetoprotein. See a -FetoproteinAmcebae, freshwater and soil, pathogenicity of Legionellapneumophila for, 1179

Amoebic liver abscess, counter-immunoelectrophoresisas screening test for, 33

Amyloid deposits, microscopic, in heart valves, as localcomplication of chronic damage and scarring, 262

Amyloidosis, senile, as protean manifestation of agingprocess, 1146

Anaemia, dyserythropoietic, congenital, type II (HEMPAS),family study of, 1197

megaloblastic, platelet monoamine oxidase activity in,963

Anaerobic bacteria, isolation from clinical specimens,multiple selective media for, 61

Analytical goals in clinical biochemistry (I.), 902, 903Angiofollicular and plasmacytic polyadenopathy, withpseudotumourous changes and dysimmunity, 1068

Anti-A and anti-B haemagglutinins in factor VIII prepara-tions, evaluation of AutoAnalyzer method for quantita-tion of, 958

haemagglutinins in factor Vill concentrates, 954Anti-actin antibodies revealed by counterimmunoelectro-

phoresis, relation to smooth muscle and bile canicularantibodies, 876

Antibiotic sensitivities of urinary pathogens (1971-8), 853sensitivity tests, light-scattering methods for, 995

Antibiotics and antiseptics, resistance to, in Gram-negativebacteria causing urinary tract infection, 288

, direct assay in solid medium for quality control inagar-dilution sensitivity-testing, 93

Antibodies, anti-actin, revealed by counterimmunoelectro-phoresis, relation to smooth muscle and bile canicularantibodies, 876

, antisteroid, solid-phase, in quantitation of oestrogenreceptors in breast tumours, 377

, complement fixing, responses to virus infection inchildren with acute lymphoblastic leukaemia, 735

to candida, humoral, and pathogenic yeasts, distribu-tion among hospital inpatients, 750

to collagen in serum in rheumatoid arthritis, 1077to synthetic poly dAT, correlation with antibodies to

native DNA and specificity for SLE, 648to Toxoplasma gondii in serum, evaluation of ToxHA

test for detection of, 644__ to toxoplasma in serum, ELISA procedure for detection

of, comparison with other serodiagnostic tests. 640, ~~~1 for quantitation of,

635, type-specific, to herpes simplex virus, indirect micro-

immunofluorescence test for detection of, 171Antibody, indirect fluorescent antibody test in Legionnaires'

disease, effect of antigen preparation on specificity andsensitivity of, 585

responses, HLA-B8 and immunoglobulins in alcohol-related liver disease, 488

, rubella. See Rubella antibodyAntibody-coated bacteria in urine, diagnostic value and

biological significance of, 183; (L), 1012, 1013

12314*

1232

Anticoagulant therapy, oral, long-term, antithrombin IIIin patients on, 1202

maintenance control by chromogenicsubstrate assay for factor X, 445

Anti-D immunoglobulin, International Reference Preparationof, recalibration by collaborative study. 249

Antigen and antibody, e. See e antigen/antibody-, carcinoembryonic, in breast carcinomas, demonstrationby immunoperoxidase technique, 356

hepatitis B surface. See Hepatitis B surface antigenpreparation, effect on specificity and sensitivity of

indirect flouorescent antibody test in Legionnaires'disease, 585- preparations, demarcation on object slides in immuno-fluorescent antibody test, 95

Antimicrobial susceptibilities of Clostridiuni difficile, 1002Antimicrobials, prescribing for urinary tract infection,

influence of laboratory reports, 481Antiphagocytic effect of Pseudomnonas aoeroginosa exopoly-

saccharide (L), 1221Anti-rabies immunoglobulin, human, plasma for preparation

of, regional programme for collection of, 180Antiseptics and antibiotics, resistance to, in Gram-negatise

bacteria causing urinary tract infection, 288Antisteroid antibodies, solid-phase, in quantitation ofestrogen receptors in breast tumours, 377

Antithrombin levels in plasma, estimation of, comparison of3 methods, 438

--- Ill in patients on long-term oral anticoagulants, 1202API-ZYM system in identification of a and non-haemolytic

streptococci, 53A1rizona hinshawiii bacteraernia and meningitis in child, case

of, 848Arthritis, rheumatoid, anti-collagen antibodies in serum in.

1077Aspiration biopsy, fine-needle, method of processing speci-mens for electron microscopy, 1214

Association of Clinical Biochemists: Reports and Bulletins,71, 176, 261, 364, 504, 594, 625, 729, 896, 1005

AutoAnalyzer method for quantitation of anti-A andanti-B haemagglutinins in factor Vill preparations,evaluation of, 958

Autoimmune abnormalities, toxoplasmosis and retinalvein Occlusion in HLA-B8 patient (new cryopathicsyndrome), 815

Automated biochemical machines (autoanalyser, flamephotometer, blood gas analyser), microbiological surveyof, 566

- continuous-flow system for measurement of haemag-glutination kinetics, 885

- methods for identification of bacteria from clinicalspecimens, 36

Baby Unit, Special Care, Royal Free Hospital, London,epidemiological study of klebsiella infection in, 400

Bacillus cereus infections (L!, 314Bacteraemia, Arizona hinshawiii, and meningitis in child, case

of, 848Bacteraemic and endotoxic shock, 888

shock, aetiological agents and laboratory diagnosis, 888? obstetric, management and treatment of, 895

Bacteria, anaerobic, isolation from clinical specimens,multiple selective media for, 61

--and white cells, counts of, in midstream urines, signi-ficance of, 58

, antibody-coated, in urine, diagnostic value andbiological significance of, 183; (L), 1012, 1013- Gram-negative, causing urinary tract infection.antiseptic and antibiotic resistance ill, 288

identification from clinical specimens, automated

Subject index

methods for, 36Bacterial isolates and improved regime of investigation for

clinical laboratory in sinusitis, 276Bacteriology, postmortem, of lung by printculture of frozen

tissue, 474Bacteroides fragilis and B. mneloaninogenics, detection by

direct immunofluorescence, 1189Basal cell carcinoma arising in smallpox vaccination site, 134Behqet's disease and recurrent oral ulceration, acute phase

proteins, factor B and lysozyme in, 269--with endocarditis and with Budd-Chiari syn-

drome, 660Bence Jones protein, detection of, comparison of sensitivity

of immunofixation and agarose electrophoresis for, 779Beta-haemolytic. See /-HaemolyticBile canicular and smooth muscle antibodies, relation to

anti-actin antibodies revealed by counterimmunoelectro-phoresis, 876

, pathological, ionised calcium in, 86Biochemical machines, automated (autoanalyser, flame

photometer, blood gas analyser), microbiological surveyof, 566

Biochemistry, clinical. See Clinical biochemistryBiopsies of jejunal mucosa, measurement by computer-aidedmicroscopy in, 254

--, rectal mucosal, acetylcholinesterase in, comparison ofhistochemical and biochemical activity of, and histo-chemical diagnosis of Hirschsprung's disease, 336

Biopsy, aspiration, fine-needle, method of processingspecimens for electron microscopy, 1214

liver, needles for, comparative trial of, 131specimens, endoscopic, gastric, quantification of G

cell density in, and effect of shape of specimen, 513-, small intestinal, morphometry and immuno-

histochemistry of, in diagnosis of food allergy, 125touch smear and endoscopic brush cytology and biopsy

histology in diagnosis of carcinoma of lower oesophagusand cardia, evaluation of, 811

Birmingham Histopathology Data Pool, use for epidemiolo-gical analysis, 351

Bladder, urinary, suprapubic aspirates of, in infants andchildren, dipslide cultures in investigation of, 694

Blood and bone marrow smears, myeloperoxidase demon-stration without use of carcinogens in, 194

---, biochemical changes in, diagnostic significance of.and liver function in African children with sickle-celldisease, 626

cells, peripheral, cytoplasmic sacuolation of, in acutealcoholism, 1193

culture, pseudoleptospires in (L), 905cultures, smears of, modified Sandiford's counter

stain for, 794donors, HBsAg-positive, hepatitis B e antigen/antibody

in, significance of tests for, 1106gas analysis, effect of air bubbles and temperature, 864packed cell volume of, determination by centrifu-

gation, ICSH reference method for, IICSH reference standard for

(L), 905platetlet(s). See Platelct(s)rheology, 417storage in liquid nitrogen, long-term, and response

of recovered red cells to haemagglutination by viruses, 8transfusions, hepatitis B carrier state produced by, 851

thalassaemia dependent on, chronic liver diseasein, hepatitis B virts marker studies in, 949

venous or capillary, measurement of erythrocytesedimentation rate by micromethod suitable for, 1118

\vessels in diabetic peripheral neuropathy, electronmicroscopical studies of, 462

Subject index

Body fluids, human a1-microglobulin levels in, 786Bone marrow. See MarrowBones with types III and V collagens in lethal osteogenesis

imperfecta, 534Book reviews:

Advances in Cancer Chemotherapy, Vol. 1, 1979, 512Advances in Medical Oncology, Research, and Education:

Proceedings of the 12th International Cancer Congress,Vol. II, Cancer Control; Vol. IV, Biological Basis forCancer Diagnosis; Vol. V, Basis for Cancer Therapy,1; Vol. VII, Leukemia and non-Hodgkin Lymphoma;Vol. VIII, Gynaecological Cancer; Vol. IX, DigestiveCancer; Vol. X, Clinical Cancer Principal Sites, 1, 1979,799

Advances in Shock Research, Vols. 1 and 2, 1979, 315Alagille, D., and Odievre, M.: Liver and Biliary Tract

Disease in Children, 1979, 512Almeida, J. D., et al.: Manual for Rapid Laboratory Viral

Diagnosis, 1979, 907Annals of the Rheumatic Diseases, Vol. 38, Suppl. No. 1,Symposium on Reiter's Syndrome, 1979, 415

Artz, C. P., Moncrief, J. A., and Pruitt, B. A.: Burns: ATeam Approach, 1979, 204

Baum, S. J., and Ledney, G. D., eds.: ExperimentalHaematology Today, 1978, 98

Bedford, M. A.: A Colour Atlas of Ocular Tumours, 1979,203

Begemann, H., and Rastetter, J.: Atlas of Clinical Hemato-logy, 3rd ed., 1979, 906

Binder, H. J., ed.: Mechanisms of Intestinal Secretion,1979, 203

Black, D. A. K., and Jones, N. F., eds.: Renal Disease,4th ed., 1979, 1015

Blaustein, A.: Interpretation of Biopsy of Endometrium,1979, 907

Blomback, B., and Hanson, L. A., eds.: Plasma Proteins,1979, 799

Bondy, P. K., and Rosenberg, L. E., eds.: MetabolicControl and Disease, 8th ed., 1980, 907

Braun, W. E.: HLA and Disease, 1979, 1123British Medical Bulletin, Vol. 36, No. 1, Chemical Carcino-

genesis, 1980, 906Brown, S, Mitchell, F. L., and Young, D. S., eds.:

Chemical Diagnosis of Disease, 1979, 1224Bruguera, M., Bordas, J. M., and Rodes, J.: Atlas ofLaparoscopy and Biopsy of the Liver, 1979, 316

Carpenter, C. B., ed.: Clinical Histocompatibility Testing,Vol. 3,1979,203

Chanarin, I.: The Megaloblastic Anaemias, 2nd ed., 1979,799

Chandra, R. K., ed.: The Liver and Biliary System in Infantsand Children, 1979, 201

Ciba Foundation Symposia: Enzyme Defects and ImmuneDysfunction (New Series, 68), 1979, 704

Clayton, B. E., Jenkins, P., and Round, J. M.: PaediatricChemical Pathology: Clinical Tests and ReferenceRanges, 1980,1015

Clinics in Gastroenterology, Vol. 9, No. 1, Virus Hepatitis,1980, 908

Cohen, A. S., ed.: The Science and Practice of ClinicalMedicine, Vol. 4, Rheumatology and Immunology, 1979,511

Contributions to Nephrology, Vol. 15, Nephrologic Problemsof the Newborn, 1979, 315- , Vol. 17, Today's Art of Peritoneal Dialysis,

1979,316Current Topics in Pathology, Vol. 67, Carcinogenesis, 1979,798

Darmady, E. M., and Maclver, A. G.: Renal Pathology,1980,1224

1233

Dick, G., ed.: Immunological Aspects ofInfectious Diseases,1979, 203

Frey, D., Oldfield, R. J., and Bridger, R. C.: A ColourAtlas of Pathogenic Fungi, 1979, 511

Furniss, A. L., Lee, J. V., and Donovan, T. J.: The Vibrios,1979, 1014

Garcia, L. S., and Ash, L., eds : Diagnostic Parasitology:Clinical Laboratoriv Manual, 2nd ed, 1979, 608

Gardner, A. F.: Synopsis of Pathology for the AlliedHealth Professions, 1979, 416

Ghadially, F. N.: Diagnostic Electron Microscopy ofTumours, 1980, 908

Gibson, J. M.: Medical Microbiology and Pathology forNurses, 1979, 202

Gowing, N. F. C.: A Colour Atlas of Tumour Histo-pathology, 1980, 1225

Greer, C. H.: Ocular Pathology, 3rd ed., 1979, 202Hamburger, J., Crosnier, J., and Grfinfeld, J. P., eds.:

Nephrology, 1979, 415Hartley, W. G.: Hartley's Microscopy, revised (2nd)

ed., 1979, 511Howat, H. T., and Sarles, H.: The Exocrine Pancreas,

1979, 1016Humason, G. L.: Animal Tissue Techniques, 4th ed., 1979,

203Huvos, A. G.: Bone Tuniors: Diagnosis, Treatment and

Prognosis, 1979, 415International Agency for Research on Cancer: IARCMonographs on the Evaluation of the Carcinogenic Riskof Chemicals to Humans, Vol. 19, Some Monomers.Plastics and Synthetic Elastomers, and Acrolein, 1979,98

International Society of Lymphology: Lymphology:Proceedings of the Sixth International Congress, 1979,908

Jenkins, J., ed.: Machine Readable Labels in the BloodTransfusion Service, 1979, 908

Johannessen, J. V., ed.: Electron Microscopy in HumanMedicine, Vol. 8, The Liver, The Gallbladder, andBiliary Ducts, 1979, 511

Keleman, E., Calvo, W., and Fliedner, T. M.: Atlasof Human Hemopoictic Development, 1979, 1225

Lee, F. D., and Toner, P. G.: Biopsy Pathology of the SmallIntestine, 1980, 1015

Leftkovits, I., and Waldmann, H.: Limiting DilutionAnalysis of Cells in the Immune System, 1979, 607

LiVolsi, V. A.: Practical Clinical Cytology, 1980, 1016Ludwig, J.: Current Methods of Autopsy Practice, 2nd ed.,

1979, 201McAllister, H. A., Jr., and Fenoglio, J. J., Jr.: Tumors of

the Cardiovascular System, 1978, 99McComb, R. B., Bowers, G. N., Jr., and Posen, S.:

Alkaline Phosphatase, 1979, 1014Maclennan, R., et al., eds.: Cancer Registration and its

Techniques, 1978, 99MacSween, R. N. M., Anthony, P. P., and Scheuer, P. J.,

eds.: Pathology of the Liver, 1979, 907Major Problems in Clinical Pediatrics, Vol. XX, Disordersof Calcium and Phosphate Metabolism in Childhood andAdolescence, 1979, 906

Major Problems in Pathology, Vol. 11, Problems in BreastPathology, 1979, 704

, Vol. 12, Surgical Pathology of theUterine Corpus, 1980,1016

Mandell, G. L., Douglas, R. G., Jr., and Bennett, J. E.,eds.: Principles and Practice of Infectious Diseases,Vols. 1 and 2, 1979. 907

Melamed. M. R., Mullaney, P. F., and Mendelsohn, M. L.,eds.: Flow Cytometry and Sorting, 1979, 607

Melnick, M., Jorgenson, R., and Bergsma, D: Develop-

1234

mental Aspects of Craniolfacial Dl smorphologv. 1979,704

Miller, D. R., et al., eds.: Blood Diseases of InfrmncY andChildhood, 4th ed., 1978, 606

Morson, B. C., and Dawson, 1. M. P.: Gastrointe.stinalPathology, 2nd ed., 1979, 1124

Novak, E. R., and Woodruff, J. D.: Gvnecologic andObstetric Pathologv, 8th ed., 1979, 799

Nuki, G., ed.: The Aetiopathogene.sis of' Osteoarthrosis.1980, 1016

Oehmichen, M.: Mononuclear Phagocjtes in the CentralNervous SYsteml, 1978, 201

Paget, G. E., and Thomson, R., eds.: Standard OperatingProcedures in Pathologyv, 1979, 606

Parker, M. T., ed.: Pathogenic Streptococci, 1979, 416Pattison, .1. R., Bitensky, L., and Chayen, J., eds.: Quanti-

tative C.vtochemistrY and its Applications, 1979, 1016Playfair, J. H. L.: Immunolb)gv at a Glance, 1979, 1014Ray, M. B.: Hepatitis B Virus Antigens in Tissuexs, 1979,202

RiethmuLller, CG., Wernet, P., and Cudkowvicz, G., eds.:Natural and Innluced Cell-etnediated Cvtoto xicits , 1979,1015

Robbins, S. L., and Cotran, R. S.: Pathologic Basis ofDisease, 2nd ed., 1979, 1123

Ross, P. W Clinical Bacteriology, 1979, 1015Rubin, A D., and Waxman, S., eds.: The Leukaaemia Cell.

1979, 512Sandritter, W., and Thomas, C.; Color Atlas and Text-

book of Macropathology, 3rd ed., 1979, 1016Scanning Electron Microscopy, 1979, 704Schochet, S. S., and McCormick, W. F.: E~ssentiais of'

Neuropathology,, 1979, 204Sher, .J H., and Ford, D. H.: Pritnars Intracranial Nro-

plasms, 1980, 1224Shuffstall, R. M., and Hemmaplardh, B.: The Hospital

Laboratory, 1979, 99Slack, J. M., and Snyder, 1. S.: Bacieria and Human

Disease, 1978, 100Soderman, W. A., Jr., and Soderman, T. M., eds.:

Pathologic Phy.siologv, 6th ed., 1979, 1016Speller, D. C. F., ed.: Antifungal Chemotherap.v, 1980.

1016Swales, J. D.: Clinic-al Hv'perten.sion, 1979, 800Symington, Sir T., Williams, A. E., and McVie, J. G.,

eds.: Cancer: Assessment and Monitoring, 1980, 1225Taussig, M. J.: Processes in Pathologv, 1979, 416Taylor, C. R.: Hodgkin's Disease and the Lvmphomlas.

Vol. 3, 1979, 606Terry, R. J., and Allison, A. C., eds.: Parasite Immunologj,

Vol. 1, 1979, 201The, G. de, Henle, W., and Rapp, F., eds.: Oncogenesisand Herpesviruses, 111, Parts 1 and 2, 1978, 315

Thompson, E. B., and Lippman, M. F., eds.: SteroidReceptor.s and the Management of' Cancer. Vol. 1,1979, 1124

Thomson, J. M.: Blood Coagulation and Haemostasis,2nd ed., 1980, 1123

Thys, J. P., Klastersky, J., and Yourassowsky, E., eds.:Aerobic Gram-negative Bronchopneumonia~s, 1980, 1123

Tyrrell, D. A. J., et al.; Microbial Disease: The Use of theLaboratory in Diagnosis, Therapy and Control, 1979, 99

UICC Technical Report Series: Screening in Cancer,1978, 608

Ultrastructural Pathology, Vol. 1, No. 1, Jan.-Mar. 1980,1123

Valdes-Dapena, M.A.: Histology ofthc. Fetxuseand Newtborn,1979, 1014

Wallach, D. F. H.: Plasnia Memobrane.s and Disease, 1979,1015

Subject index

Walter, J. B., and Israel, M. S.: General Pathology, 5thed., 1979, 202

Wheater, P. R., Burkitt, H. G.. and Daniels, V. G.:Functional Histology: A TeAt and Colour Atlas, 1979.607

Whitby, L. G., Percy-Robb, 1. W., and Smith, A. F.:Lecture Notes on Clinical ChemistrY, 2nd ed., 1980, 1224

Whitehead, R.: Mucosal Biopsy (of the GastrointestinalTract, 2nd ed., 1979, 100

World Health Organi7ation: Environmental HealthCriteria, 14, Ultraviolet Radiation, 1979, 608

Wright, R., et al., eds.: Liver and BiliarY' Disease, 1979,1014

Zilva, J. F., and Pannall, P. R.: Clinical ChemistrY inDiagnosis and Treatment, 1979, 607

Zuckerman, A. J., comp. and ed.: A Decade of ViralHepatitis, 1980, 1123

, and Howard, C.R.: Hepatitis Viruses of Man, 1979,908

Bowel. See Intestine(s)Brain damage in fatal non-missile head injury, 1132Breast, carcinoma of, carcinoembryonic antigen demon-

strated by immunoperoxidase technique in, 356grading systems for. reproducibility of

(L), 700intraduct, recognition of (L), 797

tumours, quantitation of oestrogen receptors withsolid-phase antisteroid antibodies in, 377

Buccal smears and urine, cell samples from, preparationon polylysine-coated slides for diagnostic cytology, 309

Budd-Chiari syndrome associated with Behiet's disease, 660Bullous impetigo due to Streptococcus salivariiis, case of,

1099

Cl esterase inhibitor activity, estimation of, method for, 167C3 subcomponents on red cells coated with complement in

vitro, quantitation of, 977Calcific coronary embolisation, spontaneous, necropsy

s-ray study of, 984Calcium correction for abnormal albumin concentrations in

myocardial infarction, variations between factors used ?(L), 413

ionised, in pathological bile, 86Calculi, uric acid, and renal tubular uricosuria associated

with familial hypouricaemia, case of, 971Calicivirus infection, outbreak in mother and baby unit, 1095Campylobacter, enteropathogenic, differentiation of (L),

1122Campylobacter fetus subsp. intestinalis, human straingrown at 42 C (L), 509; (L), 603

jejuni, 3-lactamase production by (L), 904,jejuni/coli infection, serological response to, 767

, serotyping of, 762Candida, antibodies to, humoral, and pathogenic yeasts,

distribution among hospital inpatients, 750Candida albicans, prevalence and intraoral distribution in

Sjogren's syndrome, 282gimilliermondii, chromium-51 release from, in assay of

neutrophil candidacidal function, 757Candidiasis, mucocutaneous, chronic, serum-dependent

defect of neutrophil function in. 370Capillary or venous blood, measurement of erythrocyte

sedimentation rate by micromethod suitable for, 1118Carbenicillin and ticarcillin sensitivity and [-lactamase

production of Psuedomionas aeruginosa in UK (1978-79),297

Carcinoembryonic antigen in breast carcinomas, demonstra-tion by immunoperoxidase technique, 356

Carcinogens, myeloperoxidase demonstration in blood and

Subject index

bone marrow smears without use of, 194Carcinoid tumours, ovarian, substance P in, 160Carcinoma, basal cell, arising in smallpox vaccination site,

134clear cell, primary, of larynx, 1160endometrial, diagnosis of, comparison of cytological

'jet-wash' specimens and histology for, 688gastric, histogenesis of, role of intestinal metaplasia

in, 801, hepatocellular, primary, differentiation of, comparison

of various assays for detection of a-fetoprotein in, 792of breast, carcinoembryonic antigen demonstrated by

immunoperoxidase technique in, 356grading systems for, reproducibility of

(L), 700intraduct, recognition of (L), 797

of colon, mucosa adjacent to (transitional mucosa),failure to demonstrate specificity of morphological andhistochemical changes in (L), 199

of lower oesophagus and cardia, diagnosis of, evaluationof endoscopic brush and biopsy touch smear cytology andbiopsy histology in, 811

, pancreatic, serum ribonuclease as tumour marker for,1212

Cardia and lower oesophagus, carcinoma of, diagnosis of,evaluation of endoscopic brush and biopsy touch smearcytology and biopsy histology in, 811

Cell samples from urine and buccal smears, preparation onpolylysine-coated slides for diagnostic cytology, 309

Central nervous system. See Nervous system, centralCentrifugation, determination of packed cell volume of

blood by, ICSH reference method for, I, low-speed, in immune electron microscopy for entero-

virus typing, 191, reorienting gradient, in determination of rubella

specific IgM, 312Cervical and urinary cytological studies, serial, in womenundergoing renal transplantation, 990

lesions, progression and regression of, in smears fromwomen followed without initial biopsy or treatment, 517

Chlamydia trachomatis, detection in rapidly producedMcCoy cell monolayers, 591

inclusions in cycloheximide-treated McCoy cells,comparison of sensitivity of immunofluorescence andGiemsa staining of, 177

, role in genital-tract and associated diseases, 205Chloramphenicol assay in clinical specimens, comparison ofmethods for, 575

Cholic acid, serum conjugates of, in cystic fibrosis, immuno-assay of, 390

Chromium-51 release from Candida guilliermondii in assayof neutrophil candidacidal function, 757

Chromogenic substrate assay for factor X in maintenancecontrol of oral anticoagulant therapy, 445

Chromosomal marker, abnormal (DI4 q+) in alpha heavychain disease, 136

Cilia, nasal, ultrastructure of, variations in, with abnormali-ties in retinitis pigmentosa, 327

Clear cell carcinoma, primary, of larynx, 1160Clinical biochemistry, analytical goals in (L), 902, 903Clostridium difficile, antimicrobial susceptibilities of, 1002

perfringens, C. bifermentans, C. sordellii and C. para-perfringens, synergistic haemolysis test for presumptiveidentification of, 395

Coagulase-negative staphylococci and micrococci, role inocular disease, 1169

, urinary infection with, in patients undergoingprostatectomy (L), 702

Cobalamins and nitrous oxide, review of, 909Collagen, antibodies to, in serum in rheumatoid arthritis,

1077, types Ill and V, in bones in lethal osteogenesis

imperfecta, 534Colon, carcinoma of, mucosa adjacent to (transitional

mucosa), failure to demonstrate specificity of morphologi-cal and histochemical changes in (L), 199

Complement activation, detection and quantitation of,immunofixation technique for, 781

components. See Cl, C3fixing antibody responses to virus infection in children

with acute lymphoblastic leukaemia, 735Computer, dedicated, haematological data processing on,926

Computer-aided microscopy for measurement in jejunalbiopsies, 254

Condylomatous tumours of vulva, vagina, and penis,relation between histological appearance and age, 1039

Coronary embolisation, calcific, spontaneous, necropsyx-ray study of, 984

Corrections, 204, 316, 416Cortisol, urinary, free, estimation in various clinical situa-

tions, modified Cortipac procedure for, 89Coulter Counter Model S, measurement of red cell mean

corpuscular volume by, value of discrepancies for earlydiagnosis of hyperosmolar states, 430

Counterimmunoelectrophoresis, anti-actin antibodiesrevealed by, relation to smooth muscle and bile canicularantibodies, 876

as screening test for amoebic liver abscess, 33in diagnosis of Legionnaires' disease, 1174

Cryoglobulinaemia, pseudoleucocytosis due to (L), 796Cryopathic syndrome, new (HLA-B8 patient with toxo-

plasmosis, autoimmune abnormalities and retinal veinocclusion), 815

Cryostat sections, technique using, for localisation ofsulphated glycosaminoglycans in mucopolysaccharidoses,897

Cyanmethaemoglobin Refence Preparations (L), 700Cystic fibrosis, immunoassay of serum conjugates of cholic

acid in, 390Cytological screening, lysosomal naphthylamidase activity

as possible aid in, 684studies, urinary and cervical, serial, in women under-

going renal transplantation, 990Cytology, endoscopic brush and biopsy touch smear,and biopsy histology in diagnosis of carcinoma of loweroesophagus and cardia, evaluation of, 811

Cytomegalovirus inclusion disease, pulmonary, adult, caseof, 11

Cytoplasmic vacuolation of peripheral blood cells in acutealcoholism, 1193

Data collection and processing, on-line, multiple, for radio-immunoassay using micro-computer system, 493

processing, haematological, on dedicated computer,926

in microbiology, integrated, simplified systemfor, 744

Deoxyribonucleic acid. See DNADiabetes, serum immunoreactive trypsin concentrations

in children with, 871a2-macroglobulin levels in, 163

mellitus, serum pancreatic isoamylase activity in, 1102Diabetic peripheral neuropathy, blood vessels in, electron

microscopical studies of, 462Dialysis patients, false negative hepatitis B surface antigen

detection in, due to excess surface antigen and postzonephenomenon, 993

Dipslide cultures in investigation of suprapubic urinarybladder aspirates of infants and children, 694

1235

1236

DNA, native, antibody to. correlation with atitibody, tosynthetic poly dAT and specificity for SLIE, 648

Dyserythropoietic anaemia, congenital. type 11 (HEMPAS).family study of. 1197

e antigen/antibody, hepatitis B. in HBsAg positive blooddonors, significance of tests for, 1106

Electron microscopic evidence of flagella and pili on

Legionella pneutnophila, 1184-- studies of vessels in diabetic peripheral neuro-

pathy, 462---microscopy for detection of rotavirus in cell culturesof faeces, comparison with immunofluorescence (L),413

immune, with low-speed centrifugation forenterovirus typing, 191

method of processing fine-needle aspirationbiopsy specimens for, 1214

preparation of rosetted cells for, 505transmission, examination of skin window

preparations by, 697fixation and embedding of small volumes

of platelets for, 600--_--- with polyacrylamide hydrogel in detection ofvirus particles, 484

Electropherograms, acrylamide gel, thin-layer, method forrecording of, 699

Electrophoresis, agarose, sensitivity for detection of BenceJones protein, comparison with immunofixation, 779

ELISA. See Enzyme-linked immunosorbent assayEmbolisation, coronary, calcific. spontaneous, necropsy

A-ray study of, 984Embryonic and fetal material, examination in diagnostic

histopathology laboratories, 317Endocarditis associated with Behqet's disease, 660

Chlamvdia trachomatis associated with, 219infective, circulating immune complexes in, nature of,

653Endometrial carcinoma, diagnosis of, comparison of

cytological 'jet-wash' specimens and histology for, 688sampling technique, Mi-Mark, assessment of, 72

Endometrium, adenoacanthoma of, separate entity or

histological variant of adenocarcinoma?, 1064Endoscopic biopsy specimens, gastric, quantification of G

cell density in, and effect of shape of specimen, 513-brush and biopsy touch smear cytology and biopsyhistology in diagnosis of carcinoma of lower oesophagusand cardia, evaluation of, 811

Endotoxic and bacteraemic shock, 888Frnterobacteriaceae from urine, evaluation of Replireader for

identification of, 571Enterocolitis, staphylococcal, and inflammatory bowel

disease (L), 604Enteropathogenic campylobacter, differentiation of (L),

1122Enterovirus typing by immune electron microscopy with

low-speed centrifugation, 191Enzyme immunoassay and single radial haemolysis, rubella

antibody determination from heparinised finger-tipblood by, 845

Enzyme-linked immunosorbent assay for quantitation oftoxoplasma antibodies in serum, 635

for rubella antibody screening, specificityand sensitivity of (L), 200

for serodiagnosis of toxoplasmosis, 841

for toxoplasma antibody detection in serum,

comparison with other serodiagnostic tests, 640in serological diagnosis of M!vrcoplasmna

pneumoniae infection, 836Enzymes in haernolysates. pH-stat assays of, systematic

Subject index

error in, 1009Epidemiological analysis, use of Birmingham Histopathology

Data Pool for, 351--- study of klebsiella infection in special care baby unitof Royal Free Hospital, London, 400

Fpithelial dysplasia and precancerous conditions in stomach,711

Erythrocyte sedimentation rate, measurement by micro-method suitable for venous or capillary blood, 1118

--. See a/so Red cell(s)Esciherichia coli or proteus, ascending acute pyelonephritis

in rats due to, experimental model for, 408Exopolysaccharide from Pseudomonas aerligino(s a, anti-

phagocytic effect of (L), 1221

Face masks, disposable, efficiency of various types of, 1086Factor B, acute phase proteins and lysozyme in recurrent

oral ulceration and Behget's syndrome, 269Vll concentrates, anti-A haemagglutinins in, 954--- preparations, anti-A and anti-B haemagglutinins

in, evaluation of AutoAnalyzer method for quantitationof, 958- X. chromogenic substrate assay for, in maintenancecontrol of oral anticoagulant therapy, 445

-XIII deficiency, congenital, fibrin cross-linking in, 434Faecal carriage of group B streptococci, 1006

- viruses, excretion during first year of life, 774Faeces, rotavirus detection in cell cultures of, comparison

of electron microscopy and immunofluorescence for(L), 413

Fetal and embryonic material, examination in diagnostichistopathology laboratories, 317

a-Fetoprotein detection in differentiation of primary hepato-cellular carcinoma, comparison of various assays for, 792

Fibrin cross-linking in congenital factor XIII deficiency,434

Fibronectin distribution in normal and diseased kidneys,1021

Fine-needle aspiration biopsy specimens, method of proces-sing for electron microscopy, 1214

Folate and vitamin B12, determination of, evaluation ofcommercial radioisotope methods for, 234

Food allergy, diagnosis of, morphometry and immuno-histochemistry of small intestinal biopsy specimens in,125

Formalin-fixed paraffin-embedded sections in direct imm uno-fluorescence of skin, 365

G cells, gastric, density in endoscopic biopsy specimens,quantification and effect of shape of specimen, 513

Gases, blood, analysis of, effect of air bubbles and tempera-ture, 864

Gastric carcinoma, histogenesis of, role of intestinal meta-plasia in, 801

Gastrin cells. See G cellsGastrointestinal disease. Chlanpdclia trachomnatis associated

with, 219Gc-Globulin, vitamin D binding, levels and affinity in

various conditions, 966Genital-tract and associated diseases, role of C//lamrdida

traclhomatis in, 205Gentamicin radioimmunoassay, evaluation of GammaCoat

(125 1) kit for routine clinical pathology laboratory (L),509

Geriatric hospital, rotavirus infection outbreak in long-stayward of. 306

Giemsa and immunofluorescence staining of Ci/amt'dvitrachomatis inclusions in cycloheximide-treated McCoycells, comparison of sensitivity of, 177

Glomerulonephritis, mesangial proliferative, diffuse primary,

Subject index

IgM associated, 1029Glycosaminoglycans, sulphated, localisation in muco-

polysaccharidoses by technique using cryostat sections,897

Gonadal infiltration in children with leukaemia andlymphoma, 722

Gram-negative bacteria causing urinary tract infection,antiseptic and antibiotic resistance in, 288

Granulocytic leukaemia, chronic, Ph' positive, extensivebone marrow infarction followed by myelofibrosis inpatient with, 449

Granulomatous orchitis, relationship to malakoplakia oftestis, 670

Granulopoiesis, importance of phagocytcsis of polymorpho-nuclear neutrophils by macrophages in bone marrow in,1110

Guthrie papers, punch for, 900

Haemagglutination by viruses, response of red cells to,after long-term storage in liquid nitrogen, 8

kinetics, measurement of, continuous-flow system for,885

test, economical and simplified, for mass syphilisscreening, 1216

Haemagglutinins, anti-A and anti-B, in factor VIII prepara-tions, evaluation of AutoAnalyzer method for quantitationof, 958--, ~~, in factor VIII concentrates, 954

Haemangioleiomyomatous tumour of lung, case of, 81Haematological characteristics of O' thalassaemia trait in

Sardinian children, 946data processing on dedicated computer, 926effects of excessive alcohol consumption, sex-related

differences in, 3measurements in septic shock, 890

Haemodialysis, hypercoagulability not induced by inter-mittent heparin treatment in patients on, 631

Haemolysates, enzymes in, pH-stat assays of, systematicerror in, 1009

Haemolysis, radial, single, and enzyme immunoassay,rubella antibody determination from heparinised finger-tip blood by, 845

, synergistic, in test for presumptive identification ofClostridium perfringens, C. bifermentans, C. sordelliiand C. paraperfringens, 395

i-Haemolytic streptococci, grouping of, by agglutination,386

use of Streptosec test for, 691Hairy-cell leukaemia, diagnosis by tartrate-resistant acid

phosphatase activity in paraffin-embedded tissue sections,552

Head injury, non-missile, fatal, brain damage in, 1132Heart valves, microscopic amyloid deposits in, as local

complication of chronic damage and scarring, 262Heavy chain protein in serum, multiple myeloma with,immunochemical and ultrastructural study of, 936

Hemalog-D and visual leucocyte differential counts, costingand evaluation of, 1114

microscopy monocyte counts, correlationbetween (L), 602

Heparin, British Pharmacopoeial assay of, evaluation incomparison with other methods, 562

__- stability, effects of diluent, heparin activity, container,and pH, 1206

in intravenous fluids (L), 1223treatment, intermittent, hypercoagulability not induced

in haemodialysed patients by, 631Hepatitis, alcohol-related, HLA-B8, immunoglobulins and

antibody responses in, 488, chronic, in transfusion-dependent thalassaemia,

hepatitis B virus marker studies in, 949in staff of clinical laboratories (1977-78), 471B as hazard to laboratory staff, re-appraisal of, 1017

carrier state produced by blood transfusion, 851e antigen/antibody. See e antigen/antibodysurface antigen detection, false negative in

dialysis patients due to excess surface antigen and postzonephenomenon, 993

screening with commercial radio-immunoassay reagents, improved economics of, 19

Hepatoblastoma, mixed, in adult, case report and review,1058

Hepatocellular carcinoma, primary, differentiation of,comparison of various assays for detection of a-fetoproteinin, 792

Hepatocytes in porphyria cutanea tarda, demonstration ofintracytoplasmic needle-like inclusions in, 899

Herpes simplex virus, type-specific antibodies to, indirectmicro-immunofluorescence test for detection of, 171

Hirschsprung's disease, histochemical diagnosis of, andcomparison of histochemical and biochemical activityof acetylcholinesterase in rectal mucosal biopsies, 336

Histopathology Data Pool, Birmingham, use for epidemio-logical analysis, 351

laboratory, examination of larynx in, 705HLA-B8, immunoglobulins and antibody responses in

alcohol-related liver disease, 488patient with toxoplasmosis, autoimmune abnormalities

and retinal vein occlusion (new cryopathic syndrome),815

Honeywell ACS 1000 leucocyte differential counter, evalua-tion of, 145

Hospital inpatients, distribution of pathogenic yeastsand humoral antibodies to candida among, 750

Howie Code and experience with examination of sputumspecimens (L), 798

Hydatid disease in mid-Wales (P), 701Hydrocolpos with peritonitis in newborn, 679Hydroxyethyl starch 350/0 60 in normal man, changes in

molecular size distribution and post-transfusion survivalas influenced by lower degree of hydroxyethylation, 880

450/0 70, post-transfusion survival of, long-termstudy of, 155

25-Hydroxyvitamin D in plasma, estimation of, rapidsimplified method for, 197

Hypercoagulability not induced by intermittent heparintreatment in haemodialysed patients, 631

Hyperosmolar states, early diagnosis of, value of discrepanciesin measurement of red cell mean corpuscular volume byCoulter Counter Model S for, 430

Hyperthyroidism with normal serum total T3 and T4 andthyroid binding protein concentrations, 868

Hyposplenism, platelet hyperactivity in sickle-cell diseaseas consequence of, 622

Hypouricaemia, familial, associated with renal tubularuricosuria and uric acid calculi, case of, 971

Immune complexes, circulating, in infective endocarditis,nature of, 653

electron microscopy with low-speed centrifugation forenterovirus typing, 191

Immunoassay, enzyme. See Enzyme immunoassayImmunochemical and ultrastructural study of multiplemyeloma with heavy chain protein in serum, 936

Immunoelectrophoresis, detection of monoclonal immuno-globulins by, possible sources of error in, 500

Immunofixation for detection and quantitation of comple-ment activation, 781

, sensitivity for detection of Bence Jones protein,comparison with agarose electrophoresis, 779

1 237

1238 Subject index

Immunofluorescence and Giemsa staining of ChIalnvdiatrachomnatis inclusions in cycloheximide-treated McCoycells, comparison of sensitivity of, 177-, direct, in detection of Barteroicdes fragiliis and B.mnelaninogenicus, 1189

---, - -, of skin using formalin-fixed paraffin-embeddedsections, 365

--- for detection of rotavirus in cell cultures of faeces,comparison with electron microscopy (L), 413

Immunofluorescent antibody test, demarcation of antigenpreparations on object slides in, 95

Immunoglobulin, anti-D, International Reference Prepara-tion of, recalibration by collaborative study, 249

anti-rabies, human, plasma for preparation of, regionalprogramme for collection of, 180

, immunohistochemical demonstration in paraffin sectionsof tonsil and bone marrow, effects of fixation and proces-sing, 1047

--, surface and intracellular, in neoplastic lymphoid cells,immunoperoxidase staining ot, 609

--synthesis by neoplastic cells as model of clonal transi-tion from IgM to IgG synthesis, 539

- M associated primary diffuse mesangial proliferativeglomerulonephritis, 1029

-----__1rubella-specific, detection by Sephacryl S-300 gelfiltration, 1082

determination by reorienting gradient cen-trifugation, 312

Immunoglobulins, detection of, immunoperoxidase methodfor (L), 98; (L), 904

HLA-B8 and antibody responses in alcohol-relatedliver disease, 488

, monoclonal, detection by immunoelectrophoresis,possible source of error in, 500

--,--, in paraproteinaemia in neurological disease,classification of, 617

Immunohistochemical demonstration of immunoglobulinin paraffin sections of tonsil and bone marrow, effects offixation and processing, 1047

Immunohistochemistry and fixation of lymphoid tissue, 1010---and morphometry of small intestinal biopsy specimens

in diagnosis of food allergy, 125Immunoperoxidase method for detection of immuno-

globulins (L), 98; (L), 904staining (L), 1222

of rectal mucosal plasma cells, quantitative studyof influence of fixation, 361

-- of surface and intracellular immunoglobulin inneoplastic lymphoid cells, 609

--technique for demonstration of carcinoembryonicantigen in breast carcinomas, 356

techniques and controls (L), 1218, 1219Immunoreactive trypsin, serum concentrations in diabetic

children, 871Impetigo, builous, due to Streptococcus salii'arius, case of,

1099Infants and children, suprapubic urinary bladder aspirates of.

dipslide cultures in investigation of, 694Intestinal metaplasia, role in histogenesis of gastric carcinoma,801

Intestine, small, biopsy specimens, morphometry andimmunohistochemistry of, in diagnosis of food allergy,125

Intestines, inflammatory disease of, and staphylococcalenterocolitis (L), 604

monocytes in, absolute monocytecounts and statistical analysis, 917

phagocytosis and intra-cellular killing, 921

Intracytoplasmic needle-like inclusions in hepatocytes in

porphyria cutanea tarda, demonstration of, 899Intraduct mammary carcinoma, recognition of (L), 797Intralactam for detection of 1-lactamase production by

Neisseria gonorrhoeae (L), 508Intravenous fluids, heparin stability in (L), 1223Isoamylase, pancreatic, activity in serum in diabetes mellitus.

1102

Jejunal mucosa, biopsies of, measurement by computer-aided microscopy in, 254

'Jet-wash' specimens, uterine, cytological examination of,comparison with histological results in diagnosis ofendometrial carcinoma, 688

Kidney, normal and diseased, fibronectin distribution in,1021

Klebsiella infection in special baby care unit of Royal FreeHospital, London, epidemiological study of, 400

Laboratories, clinical, hepatitis in staff of (1977-78), 471microbiology, reporting practices of, 830

Laboratory apparatus, historic (Paraffin lamp) (L), 1223-- reports, influence on prescribing of antimicrobials

for urinary tract infection, 481safety (L), 200staff, hepatitis B as hazard to, re-appraisal of, 1017

8-Lactamase production by Campvlobacter jejuni (L), 904by Neisseria gonorrhoeae, I ntralactam for detection

of (L), 508Laryngeal polyps, simple, histochemical study of, 526I arynx, clear cell carcinoma of, primary, 1160

, examination in histopathology laboratory, 705Legionella pneumophila, flagella and pili on, electron

microscopic evidence of, 1184isolation of, new methods for, 581pathogenicity for freshwater and soil amoebae,

1179Legionnaires' disease, diagnosis by counterimmunoelectro-

phoresis, 1174-, indirect fluorescent antibody test in, effect of

antigen preparation on specificity and sensitivity of, 585Leucocyte differential counter, Honeywell ACS 1000,

evaluation of, 145counts, visual and mechanised (Hemalog D),

costing and evaluation of, 1114Leucocytes and bacteria, counts of, in midstream urines,

significance of, 58Leukaemia and Iymphoma, gonadal infiltration in children

with, 722, granulocytic, chronic, Ph' positive, extensive bone

marrow infarction followed by myelofibrosis in patientwith, 449

, hairy-cell, diagnosis by tartrate-resistant acid phos-phatase activity in paraffin-embedded tissue sections,552

, lymphoblastic, acute, virus infection in children with,complement fixing antibody responses to, 735

, , non-T, in childhood, sex and acid phosphatasein, 151

myelogenous, acute, with bilateral mammary glandinvolvement, 547

Light-scattering methods for antibiotic sensitivity tests, 995Liver abscess, amoebic, counter-immunoelectrophoresis

as screening test for, 33biopsy needles, comparative trial of, 131disease. See Hepatitisfunction and diagnostic significance of biochemical

changes in blood of African children with sickle-celldisease, 626

, histopathological changes after allogeneic bone

Subject index

marrow transplantation, 344Lung, haemangioleiomyomatous tumour of, case of, 81

pathology in septic shock, 891postmortem bacteriology of, by piintculture of frozen

tissue, 474Lupus erythematosus, systemic, comparative specificity

of antibody to synthetic poly dAT and antibody to nativeDNA for, 648

Lymph node morphology as function of age and site, 454Lymphoblastic leukaemia, acute, virus infection in children

with, complement fixing antibody responses to, 735- ~,, non-T, in childhood, sex and acid phosphatase in,

151Lymphogranuloma venereum, Chlamydia trachomatis associa-

ted with, 219Lymphoid cells, neoplastic, immunoperoxidase staining of

surface and intracellular immunoglobulin in, 609tissue, fixation and immunohistochemistry of, 1010

Lymphoma and leukaemia, gonadal infiltration in childrenwith, 722

Lysosomes, naphthylamidase activity, as possible aid incytological screening, 684

Lysozyme, factor B and acute phase proteins in recurrentoral ulceration and Behget's syndrome, 269

McCoy cell monolayers, rapidly produced, detection ofChlamydia trachomatis in, 591

cells, cycloheximide-treated, Chlamydia trachomatisinclusions in, comparison of sensitivity of immuno-fluorescence and Giemsa staining of, 177

a2-Macroglobulin, serum levels in diabetes, 163Macrophages, phagocytosis of polymorphonuclear neutro-

phils in bone marrow by, importance in granulopoiesis,1110

pulmonary, human, sea blue granules and surfactantturnover in, relationship to smoking, 738

Malakoplakia of testis, characteristics and relationship togranulomatous orchitis, 670

Malawi, myelomatosis presenting with tumour mass in bonein, 544

Malondialdehyde-like material in plasma, determinationof, and clinical application in strokes, 973

Mammary gland involvement, bilateral, in acute myelogenousleukaemia, 547

Marrow and blood smears, myeloperoxidase demonstrationwithout use of carcinogens in, 194

and tonsil, immunohistochemical demonstration ofimmunoglobulin in paraffin sections of, effects of fixationand processing, 1047

hypoplasia with splenomegaly in adolescence, 730infarction, extensive, followed by myelofibrosis in

patient with Ph' positive chronic granulocytic leukaemia,449

, phagocytosis of polymorphonuclear neutrophils bymacrophages in, importance in granulopoiesis, 1110

transplantation, allogeneic, histopathological changesin liver after, 344

Megakaryocytes in pleural and peritoneal fluids, prevalence,significance, morphology, and cytohistological correlation,1153

Megaloblastic anaemia, platelet monoamine oxidase activityin, 963

Melanoma, malignant, histological diagnosis and prognosisof, 101; (L), 702

Meningitis and Arizona hinshawii bacteraimia in child,case of, 848

Mesangial proliferative glomerulonephritis, diffuse, primary,IgM associated, 1029

Microbiology, data processing in, integrated, simplifiedsystem for, 744

--. See also BacteriologyMicrococci and staphylococci, coagulase-negative, role in

ocular disease, 1169Micro-computer system, multiple on-line data collection and

processing for radioimmunoassay using, 493a,-Microglobulins, human, levels in body fluids, 786Micro-immunofluorescence test, indirect, for detection of

type-specific antibodies to herpes simplex virus, 171Microphotometry, simple, 97Microscopy, computer-aided, for measurement in jejunal

biopsies, 254, visual, and Hemalog-D, monocyte counts by, correlation

between (L), 602Mi-Mark endometrial sampling technique, assessment of,72

Monoamine oxidase activity in platelets in megaloblasticanaemia, 963

Monoclonal immunoglobulins. See Immunoglobulins,monoclonal.

Monocyte counts by Hemalog-D and visual microscopy,correlation between (L), 602

Monocytes in inflammatory bowel disease, absolute monocytecounts and statistical analysis, 917

phagocytosis and intracellularkilling, 921

Morphometry and immunohistochemistry of small intestinalbiopsy specimens in diagnosis of food allergy, 125

Mother and baby unit, outbreak of calicivirus infection in,1095

Mucocutaneous candidiasis, chronic, serum-dependentdefect of neutrophil function in, 370

Mucopolysaccharidoses, localisation of sulphated glyco-saminoglycans in, technique using cryostat sections for,897

Mucosa adjacent to colonic carcinoma (transitional mucosa),failure to demonstrate specificity of morphological andhistochemical changes in (L), 199

, rectal, biopsies from, acetylcholinesterase in, com-parison of histochemical and biochemical activity of, andhistochemical diagnosis of Hirschsprung's disease, 336

~~~, plasma cells in, immunoperoxidase staining of,quantitative study of influence of fixation, 361

Mumps, serological diagnosis of (L), 702virus infection, serological studies on 40 cases, 28

Mycoplasma pneumoniae infection, serological diagnosis byenzyme immunoassay, 836

Myelofibrosis following extensive bone marrow infarctionin patient with Ph' positive chronic granulocytic leukaemia,449

Myelogenous leukaemia, acute, with bilateral mammarygland involvement, 547

Myeloma, multiple, with heavy chain protein in serum,immunochemical and ultrastructural study of, 936

Myelomatosis presenting with tumour mass in bone inMalawi, 544

Myeloperoxidase demonstration in blood and bone marrowsmears without use of carcinogens, 194

Myocardial infarction, calcium correction for abnormalalbumin concentrations in, variations between factorsused? (L), 413

Myosins in pathological and biochemical studies of perinealrhabdomyosarcoma in newborn child, 823

Naphthylamidase activity, lysosomal, as possible aid incytological screening, 684

Nasal cilia, ultrastructure of, variations in, with abnormalitiesin retinitis pigmentosa, 327

Needles for liver biopsy, comparative trial of, 131Neisseria gonorrhoeae, P-lactamase production by, Intralac-tam for detection of (L), 508

1239

Subject index

Neoplastic cells, immunoglobulin synthesis by, as model ofclonal transition from IgM to lgG synthesis, 539

lymphoid cells, immunoperoxidase staining of surfaceand intracellular immunoglobulin in, 609

Nervous system, central, abscesses of, pus from, bacteriologi-cal examination of, 66

Neurological disease with paraproteinaemia, incidence,associations, and classification of monoclonal immuno-globulins, 617

Neuropathy, peripheral, diabetic, blood vessels in, electronmicroscopical studies of, 462

Neutrophils, candidacidal function, assay by chromium-51release from Candida guilliermondii, 757

, function in chronic mucocutaneous candidiasis.serum-dependent defect of, 370

, maturity of, alkaline phosphatase as marker of, studiesin normal subjects dosed with aetiocholanolone andprednisolone, 555

, polymorphonuclear, phagocytosis by macrophages inbone marrow, importance in granulopoiesis. 1110

Newborn, hydrocolpos with peritonitis in, 679-, perineal rhabdomyosarcoma in, pathological and

biochemical studies with reference to myosins, 823Nitrogen, liquid, long-term storage of blood in, and response

of recovered red cells to haemagglutination by viruses, 8Nitrous oxide and cobalamins, review of, 909Notices, 100, 204, 800, 1124, 1225

Obstetric bacteraemic shock, management and treatmentof, 895

Ocular disease, role of coagulase-negative staphylococciand micrococci in, 1169

Oesophagus, lower, and cardia, carcinoma of, diagnosis of,evaluation of endoscopic brush and biopsy touch smearcytology and biopsy histology in, 811

Oestrogen receptors, quantitation with solid-phase antisteroidantibodies in breast tumours, 377

Orchitis, granulomatous, relationship to malakoplakia oftestis, 670

Osteogenesis imperfecta, lethal, bones with types Ill andV collagens in, 534

Ovarian carcinoid tumours, substance P in, 160

Pancreas, carcinoma of, serum ribonuclease as tumourmarker for, 1212

Pancreatic isoamylase activity in serum in diabetes mellitus,1102

Pancreatitis, necrotising, acute, histological evidence ofinitiating factor in, 1125

Paraproteinaemia in neurological disease, incidence, associa-tions, and classification of monoclonal immunoglobulins,617

Pathogens, urinary, antibiotic sensitivities of (1971-8), 853Pathology measurements, provision of facilities closer to

patient for, pros and cons of, 857Penicillin, pneumococcal strains resistant to, recognition of,

1092Penis, vulva, and vagina, condylomatous tumours of,

relation between histological appearance and age, 1039Perihepatitis, Chlamydia trachomatis associated with, 219Perineal rhabdomyosarcoma in newborn child, pathologicaland biochemical studies with reference to myosins, 823

Peritoneal and pleural fluids, megakaryocytes in, prevalence,significance, morphology, and cytohistological correlation,1153

Peritonitis with hydrocolpos in newborn, 679Peroxidase-antiperoxidase method, identification of group B

streptococci in tissue sections by, in necropsy retrospectivestudy, 1165

pH-stat assays of enzymes in haemolysates, systematic

error in, 1009Philadelphia chromosome positive chronic granulocytic

leukaemia, extensive bone marrow infarction followed bymyelofibrosis in patient with, 449

Plasma antithrombin, estimation of, comparison of 3methods, 438

cells in rectal mucosa, immunoperoxidase staining of.quantitative study of influence of fixation, 361

--constituents, eight, derived from random adult popu-lation at Cumbernauld, age- and sex-related referenceranges for, 380

- for preparation of human immunoglobulin anti-rabies, regional programme for collection of, 180

25-hydroxyvitamin D estimation, rapid simplifiedmethod for, 197

malondialdehyde-like material, determination of, andclinical application in strokes, 973

Plasmacytic and angiofollicular polyadenopathy, withpseudotumourcus changes and dysimmunity, 1068

Plastic vials, weight of, discrepancies in (L), 1220Platelet count and other haematological measurements in

septic shock, 890hyperactivity in sickle-cell disease as consequence of

hyposplenism, 622monoamine oxidase activity in megaloblastic anaemia,

963Platelets, small volumes of, fixation and embedding for

transmission electron microscopy, 600Pleural and peritoneal fluids, megakaryocytes in, prevalence,

significance, morphology, and cytohistological correlation,1153

Pneumococci, penicillin-resistant strains of, recognition of,1092

Poly dAT, synthetic, antibody to, correlation with antibodyto native DNA and specificity for SLE, 648

Polyacrylamide hydrogel before electron microscopy indetection of virus particles, 484

Polyadenopathy, angiofollicular and plasmacytic, withpseudotumourous changes and dysimmunity, 1068

Polylysine-coated slides in preparation of cell samples fromurine and buccal smears for diagnostic cytology, 309

Polyps, laryngeal, simple, histochemical study of, 526Porphyria cutanea tarda, hepatocytes in, demonstration of

intracytoplasmic needle-like inclusions in, 899Precancerous conditions and epithelial dysplasia in stomach,711

Prednisolone and aetiocholanolone. studies of alkalinephosphatase as marker of neutrophil maturity in normalsubjects dosed with, 555

Pregnancy population study of susceptibility to rubellaafter introduction of vaccination, 24

Prostatectomy, urinary infection with coagulase-negativestaphylococci in patients undergoing (L), 702

Protein, Bence Jones, detection of, comparison of sensitivityof immunofixation and agarose electrophoresis for, 779

Proteins, acute phase, factor B and lysozyme in recurrent oralulceration and Behqet's syndrome, 269

Proteinuria, selectivity of, radial diffusion technique formeasurement of, 598

Proteus or Escherichia coli, ascending acute pyelonephritisin rats due to, experimental model for, 408

Pseudoleptospires in blood culture (L), 905Pseudoleucocytosis due to cryoglobulinaemia (L), 796Pseudomonas aeruginosa, carbenicillin and ticarcillin

sensitivity and f-lactamase production in UK (1978-79),297

exopolysaccharide, antiphagocytic effect of (L),1221

Pulmonary cytomegalovirus inclusion disease, adult, caseof, 11

1240

Subject index

macrophages, human, sea blue granules and surfactantturnover in, relationship to smoking, 738

Punch for Guthrie papers, 900Pus from abscesses of central nervous system, bacteriological

examination of, 66Pyelonephritis, acute, ascending, due to Escherichia coli

or proteus in rats, experimental model for, 408

Quality control in agar-dilution sensitivity-testing by directassay of antibiotic in solid medium, 93

Radial diffusion technique for measuring selectivity ofproteinuria, 598

haemolysis, single, and enzyme immunoassay, rubellaantibody determination from heparinised finger-tipblood by, 845

Radioimmunoassay, multiple on-line data collection andprocessing using micro-computer system for, 493

of gentamicin, evaluation of GammaCoat (1251) kitfor routine clinical pathology laboratory (L), 509

of serum conjugates of cholic acid in cystic fibrosis, 390reagents, commercial, HBsAg screening with, improved

economics of, 19Radioisotope methods, commercial, for determination of

folate and vitamin B12, evaluation of, 234Rectal mucosal biopsies, acetylcholinesterase in, comparison

of histochemical and biochemical activity of, and histo-chemical diagnosis of Hirschsprung's disease, 336

plasma cells, immunoperoxidase staining of,quantitative study of influence of fixation, 361

Red cell deformability, assessment by simple filtrationmethod, 373

mean corpuscular volume, measurement byCoulter Counter Model S, value of discrepancies forearly diagnosis of hyperosmolar states, 430

. See also ErythrocyteRed cells coated with complement in vitro, quantitation ofC3 subcomponents on, 977

recovered after long-term storage of blood inliquid nitrogen, response to haemagglutination by viruses,8

Renal transplantation, serial urinary and cervical cytologicalstudies in women undergoing, 990

tubular uricosuria and uric acid calculi associated withfamilial hypouricaemia, case of, 971

Replireader, evaluation for identification ofEnterobacteriaceaefrom urine and recording of sensitivity tests by agardilution method, 571

Retinal vein occlusion, toxoplasmosis and autoimmuneabnormalities in HLA-B8 patient (new cryopathic syn-drome), 815

Retinitis pigmentosa, ultrastructural abnormalities ofnasal cilia in, 327

Rhabdomyosarcoma, perinea!, in newborn child, pathologi-cal and biochemical studies with reference to myosins, 823

Rheumatoid arthritis, anti-collagen antibodies in serum in,1077

Ribonuclease in serum as tumour marker for pancreaticcarcinoma, 1212

Rosetted cells, preparation for electron microscopy, 505Rotavirus detection in cell cultures of faeces, comparison

of electron microscopy and immunofluorescence for(L), 413

infection (L), 905, outbreak in long-stay ward of geriatric hospital,

306Rubella antibody determination from heparinised finger-tip

blood by single radial haemolysis and enzyme immuno-assay, 845

, ELISA screening for, specificity and sensitivity

1241

of (L), 200susceptibility in pregnant population after introduction

of vaccination, 24Rubella-specific IgM detection by Sephacryl S-300 gel

filtration, 1082, determination by reorienting gradient centrifu-

gation, 312

Safety in laboratories (L), 200Salmonella serotyping, mechanised microtechnique for,595

Sandiford's counter stain, modified, for smears of bloodcultures, 794

Sarcoidosis, biochemical findings in, 528Sardinian children, thalassaemia trait in, haematological

characteristics of, 946Sensitivity tests performed by agar dilution method, evalua-

tion of Replireader for recording of, 571Sephacryl S-300 gel filtration in detection of rubella-

specific IgM, 1082Septic shock (Symposium), 888

lung pathology in, 891platelet count and other haematological measure-

ments in, 890Serological diagnosis of mumps (L), 702

of Mycoplasma pneumoniae infection by enzymeimmunoassay, 836

of toxoplasmosis, ELISA for, 841response to Campylobacter jejuni/coli infection, 767studies on 40 cases of mumps virus infection, 28tests, saving in numbers by pooling of sera, evaluation

of, 1120Serotyping of Campylobacter jejuni/coli, 762

of salmonella, mechanised microtechnique for, 595Serum conjugates of cholic acid in cystic fibrosis, immuno-

assay of, 390heavy chain protein in, multiple myeloma with,

immunochemical and ultrastructural study of, 936immunoreactive trypsin concentrations in diabetic

children, 871in rheumatoid arthritis, anti-collagen antibodies in,

1077a2-macroglobulin levels in diabetes, 163pancreatic isoamylase activity in diabetes mellitus, 1102ribonuclease as tumour marker for pancreatic car-

cinoma, 1212total T3 and T4 and thyroid binding protein concen-

trations, normal, in hyperthyroidism, 868, toxoplasma antibodies in, ELISA procedure for

detection of, comparison with other serodiagnostic tests,640

~~9 ~ ~J, for quantitation of, 635Toxoplasma gondii antibodies in, evaluation of ToxHA

test for detection of, 644__ vitamin B12 assay, interlaboratory comparison of, 243Serum-dependent defect of neutrophil function in chronicmucocutaneous candidiasis, 370

Sex and acid phosphatase in childhood non-T lympho-blastic leukaemia, 151

Sex- and age-related reference ranges for 8 plasma constitu-ents derived from random adult population at Cumber-nauld, 380

Sex-related differences in haematological effects of excessivealcohol consumption, 3

Shock, bacteraemic, aetiological agents and laboratorydiagnosis, 888

and endotoxic, 888obstetric, management and treatment of, 895

septic (Symposium), 888, lung pathology in, 891

Subject index

, platelet count and other haematological measure-ments in, 890

Sickle-cell disease, liver function and diagnostic significanceof biochemical changes in blood of African children with,626

-- ~, platelet hyperactivity in, as consequence ofhyposplenism, 622

Sinusitis, bacterial isolates and improved regime of investi-gation for clinical laboratory in, 276

Sjdgren's syndrome. pi evalence and intraoral distributionof Candida albicans in, 282

Skin, immunofluorescence of, direct, using formalin-fixedparaffin-embedded sections, 365

window preparations, examination by transmissionelectron microscopy, 697

Smallpox vaccination site, basal cell carcinoma arising in,134

Smoking, relationship to sea blue granules and surfactantturnover in human pulmonary macrophages, 738

Smooth muscle and bile canicular antibodies, relation toanti-actin antibodies revealed by counterimmunoelectro-phoresis, 876

Splenomegaly with marrow hypoplasia in adolescence, 730Sputum specimens, examination of, experience with, in

relation to Howie Code (L), 798Staphylococcal enterocolitis and inflammatory bowel

disease (L), 604Staphylococci and micrococci, coagulase-negative, role in

ocular disease, 1169--, coagulase-negative, urinary infection with, in patients

undergoing prostatectomy (L), 702Stomach, precancerous conditions and epithelial dysplasia in,

711Streptococcal grouping kits, commercial, correlation between

Streptosec and Streptex (L), 1222Streptococci, a and non-haemolytic, identification byAPI-ZYM system, 53

group B, faecal carriage of, 1006identification of, evaluation of rapid

methods for, 302, , in tissue sections by peroxidase-antiperoxidase method in necropsy retrospective study,1165

C, serious infection due to, 188-3-haemolytic, grouping of, by agglutination, 386

use of Streptosec test for, 691'indifferent', identification of, shortened scheme for,

47; Erratum, 204Streptococcus pneumoniae, new selective medium for, 770

penicillin-resistant strain of, isolation of, 1092salivarius, bullous impetigo due to, case of, 1099

Streptosec test for grouping of beta-haemolytic streptococci,use of, 691

Strokes, clinical application of determination of plasmamalondialdehyde-like material in, 973

Substance P in ovarian carcinoid, 160Suprapubic urinary bladder aspirates of infants and children,

dipslide cultures in investigation of, 694Syphilis, mass screening for, economical, simplified haemag-

glutination test for, 1216

Tartrate-resistant acid phosphatase activity in paraffin-embedded tissue sections, diagnosis of hairy-cell leukaemiaby, 552

Temperature and air bubbles, effect on blood gas analysis,864

Testis, malakoplakia of, characteristics and relationship togranulomatous orchitis, 670

3-Thalassaemia dependent on transfusions, chronic liverdisease in, hepatitis B virus marker studies in, 949

---trait (a3 type) in Sardinian children, haematologicalcharacteristics of, 94

Thyroid hormones (T3 and T4), total, and thyroid bindingprotein, normal serum concentrations in hyperthyroidism,868

Ticarcillin and carbenicillin sensitivity and o3-lactamaseproduction of Pseudomonas aeruginosa in UK (1978-79),297

Tonsil and bone marrow, immunohistochemical demonstrationof immunoglobulin in paraffin sections of, effects of fixationand processing, 1047

ToxHA test for detection of antibodies to To.voplaxsmagondii in serum, evaluation of, 644

Toxoplasma antibodies in serum, ELISA procedure fordetection of, comparison with other serodiagnostic tests,640

for quantitation of, 635Toxoplasma gondii antibodies in serum, evaluation ofToxHA test for detection of, 644

Toxoplasmosis, autoimmune abnormalities and retinalvein occlusion in HLA-B8 patient (new cryopathic syn-drome), 815

serodiagnosis of, ELISA foi, 841Transplantation, renal, serial urinary and cervical cytological

studies in women undergoing, 990Treponema pallidum, isolation of, method for (L), 798Trypsin, immunoreactive, serum concentrations in diabetic

children, 871Tumour, haemangioleiomyomatous, of lung, case of, 81Tumours, carcinoid, ovarian, substance P in, 160

, condylomatous, of vulva, vagina, and penis, relationbetween histological appearance and age, 1039

of breast, quantitation of oestrogen receptors withsolid-phase antisteroid antibodies in, 377

Ulceration, oral, recurrent, and Behqet's syndrome, acutephase proteins, factor B and lysozyme in, 269

Uricosuria, renal tubular, and uric acid calculi associated withfamilial hypouricaemia, case of, 971

Urinary and cervical cytological studies, serial, in womenundergoing renal transplantation, 990

bladder. See Bladder, urinaryfree cortisol estimation in various clinical situations,

modified Cortipac procedure for, 89infection with coagulase-negative staphylococci in

patients undergoing prostatectomy (L), 702pathogens, antibiotic sensitivities of (1971-8), 853tract infection, Gram-negative bacteria causing,

antiseptic and antibiotic resistance in, 288prescribing of antimicrobial for, influence

of laboratory reports, 481Urine and buccal smears, cell samples from, preparation on

polylysine-coated slides for diagnostic cytology, 309? antibody-coated bacteria in, diagnostic value and

biological significance of, 183; (L), 1012, 1013, Enterobacteriaceae from, evaluation of Replireader for

identification of, 571- specimens, midstream, bacterial and white cell counts

in, significance of, 58

Vaccination, susceptibility to rubella in pregnant populationafter introduction of, 24

Vagina, vulva, and penis, condylomatous tumours of,relation between histological appearance and age, 1039

Venous or capillary blood, measurement of erythrocytesedimentation rate by micromethod suitable for, 1118

Vials, plastic, weight of, discrepancies in (L), 1220Virus infection in children with acute lymphoblastic leukae-

mia, complement fixing antibody responses to, 735-- particles, detection by electron microscopy with

1242

Subject index 1243

polyacrylamide hydrogen, 484 clinical conditions, 966Viruses, faecal, excretion during first year of life, 774 Vulva, vagina, and penis, condylomatous tumours of, relation

, haemagglutination by, response of red cells to, after between histological appearance and age, 1039long-term storage in liquid nitrogen, 8

Vitamin B,2 and folate, determination of, evaluation of Wales, Mid-, hydatid disease in (L), 701commercial radioisotope methods for, 234 White cell(s). See Leucocyte(s)

serum, assay of, interlaboratory comparison of,243 Yeasts, pathogenic, and humoral antibodies to candida,--D binding globulin levels and affinity in various distribution among hospital inpatients, 750

Broadsheets prepared by the Association ofClinical Pathologists31 Investigation of haemorrhagic stateswith special reference to defects ofcoagulation of the blood 1961(Revised 1967) EK BLACKBURN

33 The laboratory detection of abnormalhaemoglobins 1961 H LEHMANN andJAM AGER (revised 1975 byH Lehmann and RG Huntsman asThe laboratory detection ofhaemoglobinnopathies)39 Investigation of haemolytic anaemia1961 (Revised 1968) JG SELWYN

41 Serological test for syphillis (Revised1969) AE WILKINSON

42 Determination of red cell enzymes(Revised 1970) CA WEST and TAXFRANKERD

43 Mycological techniques 1962(Reprinted 1966) RW RIDDELL54 The investigation of blood transfusionreactions 1966 (Revised 1974)GH TOVEY and WA GILLESPIE

55 Antibiotic sensitivity tests by diffusionmethods 1966 (Revised 1972)E JOAN STOKES andPAMELA M WATERWORTH

56 Haemoglobinometry by thecyanmethaemoglobin method 1967SM LEWIS

57 Compatibility test for bloodtransfusion 1967 GH TOVEY andWJ JENKINS

59 Techniques for the histologicalexamination of the eye 1967 N ASHTON

61 Screening tests for inborn errors ofamino acid metabolism 1967DC CUSWORTH

62 Preparation of undecalcified bonesections 1968 HA SISSONS

63 Tests of combined bactericidal action1969 LP GARROD and PAMELA MWATERWORTH

64 Histochemical enzyme techniques1969 IMP DAWSON and MI FILIPE(This Broadsheet is illustrated by eightcolour transparencies which can beobtained from Dr J Burston, Depart-ment of Histopathology, CentralLaboratory, St Mary's General Hospital,Milton Road, Portsmouth P03 6AG.Price £3-00, including postage.)65 The immunofluorescent test forserum antinuclear factor 1969EL HOLBROW and GD JOHNSON

66 Cytological techniques for serous fluids1970 Al SPRIGGS and OAN HUSAIN

67 The laboratory investigation of mus-cular dystrophy 1970 v DUBOWITZ,JN WALTON, BP HUGHES, andJN CUMMINGS

68 Simplified radial immunodiffusion1970 JR HOBBS

69 Immunofluorescence techniques inimmunopathology and histopathology1971 J SWANSON BECK

70 (Revised Broadsheet 36) Quantitativedetermination of porphobilinogen andporphyrins in urine and porphyrins infaeces and erythrocytes 1971C REMINGTON

71 The British comparative thrombo-plastin: The use of the nationalthromboplastin reagent for uniformityof laboratory control of oral anti-coagulants and expression of results1970 L POLLER

72 The preparation of ultra-thin sectionsfor light microscopy 1970CE LUMSDEN and SR APARICIO

73 Nervous system techniques for thegeneral pathologist 1970 J HUME ADAMand LEONARD MILLER

74 Techniques for chromosomalanalysis 1971 SYLVIA D LAWLER

75 Quality control in haematology 1973I CAVILL and A JACOBS

76 A guide to the choice of opticalequipment reagents for inimuno-fluorescence techniques 1973CED TAYLOR, AH TOMLINSON, andGV HELMER

77 Diagnosis of vitamin B12 deficiency1973 I CHANARIN

78 Radioisotopic assay of vitamin B12 inserum 1973 JL RAVEN and P BARKHAN

79 Diagnosis of folate deficiency 1973AH WATERS and DL MOLLIN

80 Estimation of bacteria and whitecells in the urine 1973 w BRUMFITT,A PERCIVAL, and JD WILLIAMS

81 Blood culture technique 1974E JOAN STOKES

82 Estimation of catecholamine metabo-lites in urine 1974 M SANDLER andCRJ RUTHVEN

83 Platelet function tests 1975JH SANDERSON

86 Photomicrography 1977RS FAWKES and AC LENDRUM

87 Estimation of plasma cholinesteraseactivity and the use of inhibitors for thedetermination of phenotypes 1977MARY WHITAKER88 Examination of cerebrospinal fluidprotein 1978 GH GRANT89 Handling and clinical use of bloodproducts 1978JOHN WALLACE and JOHN F DAVIDSON90 Practical aspects of anti-Dprophylaxis of haemolytic diseases of thenewborn 1978 w WAGSTAFF91 Examination of a decomposed body1979 DJ GEE92 Diagnosis of amyloidosis 1979CR TRIBE and VA PERRY93 Investigation ofmucopolysaccharidoses 1980CA PENNOCK94 Erythrocyte sedimentation rate andplasma viscosity 1980 SM LEWIS95 Estimation of serum digoxin byradioimmunoassay 1980MARY MCMILLAN, GP MOULD,HJ PUXLEY and VR THURLOWNotes on sterilization No. 1 Sterilizationand disinfection of cytoscopes 1967Notes on establishment ofa hospitalradioactive tracer service 1969Reprint No. 1 (J Clin. Pathol., 22, 383)The histology and cytology of changesin the epithelium of the cervix uteri1969 ADT GOVAN, RM HAINES,FA LANGLEY, CW TAYLOR, andAS WOODCOCKReprint No. 2 (J Clin. Pathol., 24, 159)The perinatal postmortem examination1971 FA LANGLEY

Reprint No 3 (J Clin Pathol., 27, 590)The use of SI units in reporting resultsobtained in hospital laboratories 1974DN BARON, PMG BROUGHTON,M COHEN, TS LANSLEY, SM LEWIS,and NK SHINTON

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