ABC VetUserManualPart number: RAB 015A Ind.E

a

tPr

'P"\

-- \ tb''rr\O\

ABX "f:ilff;

abc*atful&'et,:,

nt" A" Caducee Parc buromddecine 34184MONTPELLIER Cedex 04 FRANCE::,:' ,:::=:.

,, Tel.:(33)4 ',6714 is fO :': Fax : {33) 4:,67 1415 17

l:

:-:::

REVISIONS

^ffiA B RAH533 AA RAH569 AA RAH536 AA RAH634 AA RAH653 AA X Creation version V1.0 Softwareall all 15.12.96 19.06.98

V1.42 version Update software ABCVet16 parameters creck c erwi Electromagneti ronment V210 version soltware Update V2.21 version software Update of Newcertificate conformity

cD E

all

22.09.98

toutesX

01,07.99 22.02.01

i,.:.ri '.i:.4H

ffi:,o+;$

fr,it+i.q,m*

DECLARATION CON FORMITE DE DECLARATION CON FORMITY OFACcoRDrRit% EN45014DIAG N OSTI CS

ParcEurom6decine ruedu Caduc6e BP7290 MONTPELLIER 34184 4 Cedex FRANCE

NOUSLE FABRICANT WETHEMANUFACTURER

*X{ABXDiagnosticsAdresse ParcEurom6decine Address ruedu Caduc6e BP7290 34184MONTPELLIER Cedex 4 FRANCE DECLARONS OUELE PRODUIT DECLARE THATTHE PRODUCTNomproduit Productname Moddles Models

MICROSOT-CTOS-CS-A Vet-CHOR B C |

DIRECTIVES NORMES ET ESTCONFORMEAUX CONFORMS TO THE FOLLOWING DIRECTIVESAN D STANDARDS Directives 89/336/CEECEM Directives 89/336/EEC EMC 73/23/CEEBasse Tension 73/23/EEC Low voltageNormes t E C 0 ' l - 1 d . 9 9 1 E 1 6 Standards EN50082-1 Ed.1992

E N5 5 0 1 1 l a s sB E d .1 9 9 1 C . E N6 1 0 0 0 - 3 - 2 E d . 1 9 9 5 EN61000-3-3 1998 Ed. DECEMBRE 2OOO DECEMBER 2OOO

FORM 98 Rev4 01

Thismanualis a guide tor the useof the ABG Yef BloodCounter Automated

and for Data thismanual been in verified validated isbelieved beadequate theintended of the to use has and AutomatedBlood Counter.lttheAutomatedBlood Counterorthe procedures usedfor the purpose are validity suitability in confirmationtheir of and should obtained be beyond capabilities the specified thismanual, doesnotguarantee results assumes obligation no or fromABXDiagnostics; ABX and otherwise Diagnostics nor liability. publicationnota license operate This is under a recommendation to infringe upon product any to patents. or process

HAZARDS POTENTIAL features guard operator frominjury, instrufhe Automated safety to the the Blood Counterincludes several frominaccuracies. alertthe operator potentially To hazardous mentfromdamage of and the test results whicharedescribed is wherever necessary headings below provided conditions, of theboldcaptioned one throughout text. this

VUARNING I

Flags a procedure that if not followed properly,can prove to be extremely or hazardousto either the operatoror the environment both.

; CAUTION

I Emphasizesoperating procedures that must be followed to avoid possible to | Oamage or destructionof the instrument.

IMPORTANT

Emphasizes operating proceduresthat must be followed to avoid erroneousresults.

operational function. during aftera specific or

Tableof contentsSection1: lntroduction

Section2 : lnstallation

Section3 : Specifications

Section4 : Technology

Section5 : Description Section6 : Startupand sample run

Section7 : Calibration

Section 8 : Instrumentconfiguration

and troubleshooting Section9 : Maintenance

\_./-

1. INTRODUCTION

CONTENT

-)

1. INTRODUCTION1.1.INTENDED E US (Microprocessor controlled) is Veterinary)a fullyautomated Blood Counter TheABG Vet(Animal specimens. of blood diagnostic testing whole forthein vitro used hematology analyzer hematology according itsinternal to system as TheABG Vetis available a 8 or 16parameters reagents: setup, anduseof specific ABC Vet 8p: - WBC,RBC,PLT, - HGB,HCT, - MCV, MCHC, MCH, - RBCandPLTdistribution curves. ABC Vet 16p: - WBC,LYM7., MON#, MON%, GRA%, GRA#, LYM#, - RBC,HGB,HCT, RDW, PLT, MPV, MCH,MCHC, MCV, RBC,PLT curves:WBC, and3 distribution fromEDTA). from12 pl of wholeblood(taken

IMPORTANT

- TheABG VetBP in instrumentis available "bottle" and "pack" instrument Upes. - The ABC Vet 16P(LMGE) in is instrument available "pack" instrument type only.

WBC : RBC HGB HCT: MCV MCH MCHC RDW: PLT: M P V: LYM% LYM#: MON% MON#: GRA% GRA#:

cellcount White blood cellcount Redblood Hemoglobin Hematocrit Mean cellvolume hemoglobin Mean corpuscular concentration hemoglobin Mean corpuscular width Reddistribution Platelet count plateletvolume Mean percent Lymphocyte number Lymphocyte percent Monocyte number Monocyte percent Granulocyte number Granulocyte

of per Thisrateincludes timefromintroduction the is42 Therateof determinations samples hour. is The the and and analysis displaying printing results. system to blood sample sample thewhole printer recording test for all anda graphic system dilution with totally automated, an internal printouts. flags results including andgraphic

1. INTRODUGTION

1.2.PRESENTATION in Theinstrument, whichis small size,has7 mainparts: 1 - Theelectrical supply. 2 - Theelectronic board. pneumatics. 3 - Thedilution panel including keyboard a LCDscreen. and 4 - A control a - A reagent 5 compartment. and of curves. out 6 - A printer prints theresults the plotting thedistribution that - A smartcardreader select bloodspecies, runcontrole results. the to bloodandto record 7 to (Diag1.1). at together onepanel, thefrontof thesystem on Allthecontrols grouped are

Diag.l-01

1.3. NOTES Your retains rightto makechanges the instrument thisdocument. to and ABX Diagnosfics the provide latest office revision thisdocument. the of local ABX Diagnosfibsrepresentative will prior or in without may Nopartof thisdocument becopied reproduced anyformor by anymeans written consent ABX Diagnosfics. of

qq

2. INSTALLATION

qo

2. INSTALLATION2.1. INSPECTION it. sending We, nevertheless, out is A thorough inspection carried on tne ABC Vet before to any to as the recommend checking totalsystem soonas it is received report anomalies the in procedures be mustimperatively followed the ordergivenbelow. The carrier. installation 2.2.UNPACKING protective in foambefore beingplaced a cardboard in is The instrument enveloped a special, Remove cardboard containing box the the fourangles theboxto unpack system. of box.Cutthe (seeDiag.1). kit tne ABC Vet installalion fromits location

Diag.2-01 type according the instrument : to kits Twodifferent installation areavailable - A ABC Vet "bollle" kit installation - A ABC Vet "pack" kit installation : specifications printers available to according thecountry Twodifferent are - Printer : (220V,50/60H2) model European - Printer model (110V, : 50/60H2) US XAA398A XAA399A

2. INSTALLATION2.3.PACKAGE CONTENT - TheABC Vetboxes parts : the contain following ABC Vet Printer (english) manual User's "Dog"Smart card "Cat"Smart card "Horse" Smart card"BloodControl" Smaftcard

8P

16P

R A B0 1 5A 20520 12 20520 13 20520 14 205 20 32 D A C0 1 1A D A C0 1 2A

R A B0 1 5A 20520 63 20520 64 20520 65 20520 73 D A C0 1 1A D A C0 1 2A

(european) Power cable or (US) cable Table 2-01 Power

- TheABC Vet "bollle" kit : installation includes DESIGNATION ReagentstrawL=270mm needle Sampling DiluenUwaste connector male screws Connector Cristaltube 3x6 O ring 6x1.5 1 Oring ,4x1,25 2 Rubber stopper holeskit 2-02 Commoni nstallation Table

PART NUMBER XEAOlTA GBC069A EAC OO9 A EAC OO7 A E A E0 1 1A FAA036 A FAA053 AF B LO O 1 A

OTY 31

2

241

2 3II

X E A3 1 2A

- TheABC Vet "pack" kit : installation includes DESIGNATIONneedle Sampling

PARTNUMBER GBC069A FAA036 A FAA053 A X E A3 1 2A

QTY 1

Oring6x1.5 O ring1,4 x 1,25 Table 2-03 kit installation Common

2 21qq

2. INSTALLATION- The common : kit installation (XEA312A) includes DESIGNATION Fuse1A,220V5P.0 Bentwrench 2.0 Bentwrench 2.5 Bent wrench 1.5 Allen 2.5 key Tox keyT10 Tygon tube1.52 Tygon lube2.29 T210-6 Fitting 1.6mm T PAHTNUMEERDAR O4O A MAB OOlA

ATY2 1I I

MAB OO2 A MAB OO3 A MAB 069 AMAB O9O A

1 1

EAE OO7 A EAE OO9 A EAB032AF A A0 1 7 A

2 2I

o ring30.8x3.6.5 O ring15x1 KM Grease 1011 ABC Vetcover Table 2-04 ABC Vetcoverkey

1 1 1 1 1

FAA029 A X E A0 1 9A F B H0 1 5AFAJ OO4 A

2.4.WORKING CONDITIONS Please notethatABC table workstation. or andlevel on ABC Vel should placed a clean be (66lbs).Avoidexposure sunlito 30 weighapproximately kilograms and reagents Vet, prinler must leftbehind the 20 be of ght.Proper ventilation requires a space at least cm (8 inches) that apparatus. check Electromaqnetic 2.4.1. environment levelof electromagnetic lessthanthe required to The ABC Vet hasbeendesigned produce The interferences to in in interferencesorder operate conformity itsdestination. electromagnetic to in operation otherinstruments of levelallowing correct the to by the ABC Vet arelimited a with r conformity thei destination. is in of check theinstrument notplaced theproximiy electromagnetic that Incaseof problems, (radars, scanner, etc...). X-rays, waveemissions fields, short or protection 2.4.2.Environment in mustbe collected a plantspecialized by and Whenold the instruments theiraccessories according thelegislation. to of and elimination recycling thiskindof equipment 2.5.GROUNDING (earth) plugis correctly to connected grounding required. that is Check thewallground Proper lf thereis no ground stake. thenusea ground grounding installation. electricity the laboratory mustbe applied. norms Current electricity

2. INSTALLATION2.6.HUMIDITY TEMPERATURE AND CONDITIONS 18 ABC Vet 8p can function between to 32'C (65 to 90'F),ABC Vet I6p can function humidity, meaning than85%withnocondenless 20 between to 30'C(68to 86'F),withrelative (50'F), instrument less lf the to sation. it is kept a temperature than1O'C at should allowed sit be roomtemperature before use. for an hourat thecorrect 2.7.VISUAL CHECKS 2.7.1. Mechanical check kit, into in the as on Using keycontained theinstallation turnthelocker shown theDiag.2.2 order protection door. to open pneumatic the

Diag.2-02 f andremove cover: pullit backward liftit upto the the Unscrew 5 cover ixation screws and the (Diag. rear theinstrument 3). of

Diag.2-03qcl

2. INSTALLATIONto locking (A) as far as possible the leftand placethe sample clip Pushthe plastic carriage in here (B) to side, as needle carriage as farforward possible theright-hand as shown Diag.4, position. (C) sureit is in itsupper that below. Check the needle is notbentandmake .J

Diag.2-04

in Each be below. chamber should in its as of Check position thechambers shown Diag.S the is firmly the RBCchamber. block attached to proper position itsclips theelectrode with and

Diag.2-05 block 1 - Electrode 3 - RBCchamber 2 - Clip 4 - Mixing chamber

2. INSTALLATIONprotection chamber cover.Check thatthe of Unscrew slightly 2 screws theWBC/Hgb the firmlyto the chamber in blockis attached is chamber fixedproperly its clipsandthe electrode (Diag.6).

Diag.2-06 2.7.2. Connection check circuit in 7). boardaresecurely place(Diag. on Check theconnectors the printed that

+nDiag.2-07

2. INSTALLATIONpressing theholder (Diag. lock 8) on on f Remove fuseholderromitslocation therearpanel the 220 Slow-Blow' be : they the andcheck fusecharacteristics should 1 Ampere, Volts

*HY|H'@-9

Diag.2-08

cover. coverandthe instrument chamber Re-install HGB^ruBC the CONNECTIONS 2.8.REAGENT : packages available are Tworeagent - Bottles containers (ABCVetSP only). and - Reagent pack. . 2.8.1Bottleconnections in 9. as compartment shown theDiag. the inside reagent are reagents placed Lyse cleaning and and bottle thebluetubeto thecleaner Connect stoppers. and Install reagent straws thebottle the cover' the Close compartment thewhite tubeto thelysebottle.

Diag.2-9

2. INSTALLATION: CAUTION I The diluent containerwill be locatedon the bench at the same level than the

instrument.

in kit input output included theinstallation attheliquid located at Install male and the connectors (Diag. 10). rear thebottom theinstrument panel of (see above) tube using diluent the straw a 3x6cristal and Connect diluent the container CAUTION (1 metermaximum) the diluent inputlocated the bottom the instrument panel. rear at of on using cristaltubex 6 onthewaste the 3 output, place waste and the Connect waste the container level bench). container below instrument (underthe the

DILUANT DECHETS DILUENT I4IASIES

Diag.2-10

procedures wastedisposal,Neverconnect for Alwaysfollow the recommanded drain pipes,Foreachwasteconthe instrumentwastesdirectlyto the laboratory procedureas describedin section2.9. tainer,follow the neutralization packconnection 2.8.2. Reagent in TheReagent Packincludes entire of reagents a "Pack" is ableto receive waste the set and the pockets by liquids. Three contain 3 reagents the diluent, cleaner lyseandareclosed and soft pocket empty located thebottom thepack. fourth at The is and means thevalveconnectors of of liquids. is intended receiving for waste plateinorder remove protection plugs to the located the at Push downwards thepacksupport on packlocation (Diag. 11). bottom the of

Diag.2-11 12. into of as Install pack on the directly thecompartmenttheinstrument shown theDiag. Push the to connectors. thepackdownin order plugcorrectly packon themale

qq

2. INSTALLATION

ffiDiag.2-12

valvein orderto on (see Thefreemaleconnector Diag.13)mustbe plugged the packupper liquids. receive waste the

Diag.2-13

CAUTION:

not In order to avoid leak problemsit is recommended to unplug severaltimes the same reagentpack,

2. INSTALLATIONPRECAUTIONS HANDLING 2.9.REAGENT ANDWASTE (15'Cto 25"C;59'F to77'F). Operating at Reagents haveto be stored roomtemperature 2.6. contains cyanides have be handled and to conditions givenin section Lysereagent are precautions regulations. Always followthe recommended according the localor national to (seesection for the reagent specifications). 3.3 require,waste liquids can be neutralized lf your local or nationalorEanizations beforebeing discarded. - Wastefiquidsof lhe ABCVet canbe neutralized usingthe followingprocedure: then wasteliquids,add 50mLof SodiumHydroxidesolution 200g/L, For 20L of solusolution 12'Gl daily preparedfrom the commercialized 250mLof bleach tion (48'Cl). - Gyanides add from the lysing reagent(ABC Vet8P):for 20L of lysing reagent, sotu50mLof SodiumHydroxide2}lglL,then 100mLof AmmoniumPersulfate or tion 500g/Ldaily prepared, add 50mLof SodiumHydroxide2ililglL,thenadd solution30%. 500mL SodiumHypochloride of

WARNING!

NOTE

with the pack,this one collects the wastegenerated all nstruments equipped with compliance the latest environmental inoto facilitate

2.1 ELECTRICAL O. CONNECTIONS in electrical to supply using power the cableincluded TheABC Vet is connected the laboratory 2.3). Connect power the on cable thepluglocated therearleftto installation (see kit section the (Diag. 1a). hand sideofthedevice plug. Theinstrument be operated anyother the can at under power Two1Afusesarelocated, (from to 60Hz) without modification. (from to 240V) frequency 50 100 or voltage

wiF

-o^o

Diag.2-14

SWITCH 1 - ON/OFF HOLDER 2 - FUSE PLUG SUPPLY 3 - MAIN

4 - RS232 OUTPUT 5 - PRINTER OUTPUT

qq

2. INSTALLATIONPOINTS 2.11. GENERAL .1 lt device. responds the601 to 1 TheABG Vel is classified a class , typeB electro-medical as Worksecurity reliability general and Commission. Electrotechnical normof the International under following the conditions only: by characteristics guaranteed ABX Diagnosfics are - services repairs provided a AB,,",::l,,,YE$

,;:::.::NG::

with to the and Move cursor YESto print results the limits the The* indicates current state. the ENTER. press without limits press the the and Move cursor NOto print results to ENTER. the LIMITS LABORATORY 8.2.CHANGE

IMPORTANT

Limitsare differentaccordingto the species.Theselimits are recordedon the smartcard and can be modifiedby the user (seesection8.2).A confirwhenexitingthe Change Lab.Limitsmenu. mationof the recordis required

with the limits above upper Results exceed laboratory areidentified a flag: H for results the that limit. limit, for results L below lower the is via whenthisoption computer the RSoutput to These limits betransmitted an external can (see validated section 8.5.2). move cursor thefunction to From SETUP menu, the the O : is TER. menu displayed TheLABLIMITS CHGLABLIMITS Ht{:MM Resultlow limits 8.2.1. Movethe cursor function to lil \-J : displayed menuis The LOWLIMITS f-OW LIMITS pressENTER. and ENCHGLABLIMITS press and

> 1 LOWLIMITS 2 HIGH LIMITS

:XX

message . the change requested, is The original CIALmenuto function Q : menuis displayed pressENTER. PASSWORD The anO

iess,r;r. cnGCURRHNT:. i characters if 3 Enter newpasswordnecessary,numeric the password displayed. is Thecurrent one. the or keyboard ESCto keep current maximum using numeric the Startupcycle 8.3.3. in out startup rinse anydetergent the to day every attheinstrument is TheSTARTUP cycle used any before whichmust determined be count includes background a cycle The system. STARTUP interferences that to is necessary ensure thereare no extraneous This samplesareanalyzed. of noiseandaffectthe cellcount.lf the results the bacas that may be detected background performs given a 6.3, specifications in section the analyzer the kground countare notwithin ly. P second STARTU cycleautomatical accessed or startup, manually at ThisSTARTUP cyclecanbe runautomaticallyeachinstrument fromthe needs, according theoperator's to the key. configure instrument To using @ the "ENTER". STARTUP The the menu, move cursorto function SfnnfUP andpress the SpECIAL [l \__J : menuis displayed

is "ENTER". When MANUAL setup selected, the and setup press Move cursor therequired to the : startup at will message bedisplayed theinstrument thefollowing

thentne @ Press keyto access MAINmenu the a

is keyif an analysis required.

IMPORTANT8.3.4.Inactivemode

will NOTINITIATED" be printedout with the analysis "STARTUP A message cycle is not carriedout afterthe startupof the resultswhen the STARTUP instrument.

(auto cycleautomatically mode)after4 hoursof the This modeallows userto run a startup (except menu). is SERVICE which evermenu displayed This occurs instrument inactivity. cycle the menu, move fromtheSPECIAL needs, to according theoperator's Toconfigure instrument the is menu MODE The and tVE cursorto function I trunCf MODE press'ENTER". INACTIVE the |+ \_/ : displayed

CONFIGURATION 8. INSTRUMENT>-1AUTO INACTIVFMODE . H H : M M i i , , I M A N U A L "ENTER". newsetup recorded. The is and option press Move cursor therequired to the Autocleani f requencv nq 8.3.5. (see is carried every samples out 50 solution normally involvingcleaner a Anautomatic cleaning frequency cleaning the according to The section 6.6.2). userhasthepossibilty setup automatic menu, From move cursor the run usually initslaboratory. theSPECIAL tothenumber samples of "ENTER". CLEAN. is The FREQ. menu displayed: FREQ. press and tothefunction CLEAN. llJ F . C L E A N R E Q? : CURRENT] E X I T :E S C S A V E :E N T E R

frequency is The cleaning frequency press"ENTER". newautomatic and Enter required the recorded. Internal setupprintout 8.3.6. Move cursor function the on out this Thesetup alluseroptions be printed using function. can of r^-\ (limits configuration) internalsetup and The SETUP press'ENTER". instrument and gJ PRINT is printed out. Cycleend audiblesignal 8.3.7. menu. Move cursor the (BlP) be setupwiththeSPECIAL the to A cycle can endaudible signal *ENTER". audible menu displayed is : The signal function )gUZZeR press and 7

The is and Move cursorto required option press'ENTER". newsetup recorded. the the ldentification mode 8.3.8. : Twoidentification modes available are - USmode:theoperator letters numbers of eachpatient using or must typeintheidentification The results are will out (see 6.5). section Theidentification beprinted withtheresults. STARTUP printed too. out or - Standard This running analysis an series. before a can mode: theoperator enter runnumber and out cycle printed withtheresults. runnumber beincremented each will on Move cursor thefunction the to O:..:::a:::-::a:.

"ENTER". following The menu displayed: is and 'OMODE pressiir*.ii]#f :.-.:.:.:.:.rill

rDMODEIl-tflMM - 'l 'i

i,ii

"ENTER". new"lD MODE" recorded. The is and option press Move cursorto required the the

CONFIGURATION 8. INSTRUMENTANDTIME 8.4. DATE From specifications. the SETUP to according the country Dateand time can be changed to menu, move cursor thefunction the O : menuis displayed DATET|ff*e l. i rlH:MM8.4.1.Changetime

"ENTER". DATE TIME The TIMEandpress DATE

:

Move cursorto function the the Q

menu displayed: is press "ENTER". CHANGE TIME The anO

The HH.MM press"ENTER". newtimeis recorded. and Enter required timein theformat the 8.4.2.Dateformat (the FORMAT current to move cursor thefunction DATE the TIMEmenu, From DATE the O FORMAT menuis displayed: The and is setting displayed) press"ENTER". DATE

4 different can be used: dateformats

MM.DD,YY DD.MM.YY YY.MM,DD YY.DD.MM

'ENTER'. is Thenewdateformat and selection press in Movethecursor frontof the required recorded. 8.4.3.Chanqedate DATE andpress to movethecursor the function 1CHANGE Fromthe DATE TIMEmenu, Is 'ENTER". CHGDATE menuis displayed: The

"ENTER". new The in recalled themenu andpress to Enter newdateaccording theformat the dateis recorded.

CONFIGURATION 8. INSTRUMENTOPTIONS 8.5.HOST laboratory via computer theRS232 data is of TheABG Ver capable transmitting to an external plug to to computer beconnected theanalyzer, oneendofthe interface.youhave external lf an Plug vendor) theexternal into computer. theother (provided yourcomputer by computer cable as on receptacle therearoftheinstrument shown the at into cable thecable endof thecomputer 2.10. section NOTE the specifications, to computer TheABC Vethasto be setupaccording the external computer specialist only. by have functions to beused yourlaboratory following port : of Thedefault setup theserial areasfollow :8 1 - Byte number 2-Parity:none 3 - Stopbyte: 1 4-Xon/Xoff:none and press to Fromthe SETUPmenu,movethe cursor the function O HOSTOPTIONS \__J menuis displayed: "ENTER". HOSTOPTIONS The

"ENTER" access different settings. to the and option press in Move cursor frontoftherequired the "ENTER" record newsetup. The the and to setting press in of Move cursor front therequired the : are settings asfollow different options their and . 8.5.1Hostcommunication ( 1 - F O R M A T lA B X ) 2 - FORMAT2 3 - STANDARD 4 - TR OFF(transmission OFF) 8.5.2. Sendlimits 1-YES 2-NO 8.5.3.Baud rate 1-300 2- 1240 3 -2400 4 - 4800 5 - 9600

Transmission 8.5.4. and move cursor thefunction TRANSMISSION to menu, the Fromthe HOST OPTTONS fTl \__J via laboratory computer the RS pressENTER. lastresults transmitted theexternal to are The 232.

CONFIGURATION 8. INSTRUMENT8.6.MEMORYCARD lntroduction 8.6.1. with Thismenuis available if theABG Velis equipped chipcardreader. only functions : program the TheMEMORY contains sixfollowing function. the to 1 - MEMO allows operator enable/disable memory : the identification stored is the of 2 - PRINT LIST: thepurpose thissubmenu to print listofthepatient card. on thememory only. to oneresult allows operator print the ONE: thissubmenu 3 - REPRINT recorded the on to out the allows operator print alltheresults 4 - REPRINT : thissubmenu ALL memory card. fromXXXto YYY. 5 - PRTFROMTO : printout results card. recorded the memory on alltheresults 6 - CLEAR CARD: erase to the From SETUP menumove cursor thefunction the O : is Thememory menu displayed MEMOCARD pressENTER. and

' * ''l Yfi,HF,-E?*l;i :rii ri

HH:MM:ffi

i

8.6.2.Memoon/off Fromthe Insert card into the slot in the upperleft cornerof the analyzer. the MEMORY function move cursorto MEMOCARD menu, the [l \.J menuis displayed : The ftff ftfOandpressENTER. following

on card.The the backup the memory or Thisfunction the allows userto enable disable result . indicates current function, move the To function. activate MEMO the of state the memory the - ON andpress move cursor 2 - OFFandpressENTER. lhe to To ENTER. disable cursor 1 to

IMPORTANT

modeturns to US mode.lt As soon as the MEMOis ON,the identification is mandatoryto enter the identificationbeforerunning the analysis.

CONFIGURATION 8. INSTRUMENTRunninqthe samples 8.5.3. The the Plunge probeintothe sample tubeand press sampling to startthe analysis. bar the following : menuis displayed

Pnf tp *:,t . CURRENT :Then, analyzer ENTER. if the checks a MEMO CARD Enter patient identification press and the is present. - lf the Cardhasnotbeeninserted, following message : the appears::::::::: :: ,::=, . l':::i.: i:::::.::=

$t0i'-.....= ;tt.!,.1!LQti = S i hlTEA:l

ENTER. Insert cardandpress the - lf thecardis notthe MEMORY thefollowing message warns operator one, the

Replace cardby a memory andpressENTER. one the - lf the cardhasbeenintroduced a wrongway in. tl:l

foreward on the uppersideandpressENit and Remove cardand insert withthe arrow the TER. Whenthecardis full :

ENTER. Insert newoneandpress a are on card,as wellas thepatient When analysis completed results stored thesmart is the the identification. CAUTION : I fne graphic resultsare not recordedon the card.

When oneis full,theoperator musteither replace the this 60 results be stored thecard. can on of fromthecurrent or all cardby a newoneto carry thebackup the results clear the results on card(see8.6.7). whichcorresponds the indexof the results to The results storedundera backupnumber are recorded the card. on NOTE if Results notbe recorded the MEMOis OFF. can

CONFIGURATION 8. INSTRUMENT8.6.4.Print list identifications recorded to The PRINT the LISToption allows operator printoutallthe patient (column number as on the card,the time and the date of the analysis well as the backup MEMO). ro move cursor menu, the From MEMOCARD the O ENTER. PRINT LIST andpress

vFri-lsT :Example :

l:

MEMO 1 2 3 4 5

DATE 10t23t1995 10t23t1995 10t23t1995 10t26t1995 10t26t1995

TIME '16:39 17:18 17:26 17:33 17:36

NAME 123 951 1235 784 895

Diag.8-02

8.6.5.Reprintone is with This number displayed results number. backup involves backup the Reprinting result one identification. underneath patient thet:

ii::.t::;1

the ONE and pressENTER. to movethe cursor f 3-'l PRINT menu, Fromthe MEMOCARD \__J : following menuis displayed

i,i 'GUHg#' Wtme

rVE:ESC

i ErutEn

The are out to of Enter backup number the results be printed and pressENTER. results the (on the printed withthe backup below time). number the right, out in on stored thecardis displayed CURRENT]. number results of [ NOTE I tne totat Reprintall 8.6.6. Check quantity the stored thecard. on alltheresults to Thisfunction allows operator reprint the movethe cursor to menu, ALL.Fromthe MEMOCARD of paper running REPRINT a before without out The are ENTER. results printed in theusualformat neentNTALLandpress [! graphs. (the pressESCuntilit hasbeendetected the program by lf the printout to be interrupted has printout). program checks keysonlyat the endof a result the

CONFIGURATION 8. INSTRUMENT8.6.7.Print from to on recorded thecard' only to the allows operator printout oneparlof the results Thisfunction TO movethe cursor,oo pRT FROM andpressENTER. menu, Fromthe MEMOCARD

Move cursor,o the O

: is submenu displayed :the BEGIN ENTER and BEGIN press

out to of number thefirstresult be printed andpressENTER' Typein the backup to Movethecursor O : is :the ENDsubmenu displayed ENDandpressENTER

''' ,UffrYf*'E?c' i;:.i': EU*o''-

Movethe out to of number the lastresult be printed and pressENTER' Typein the backup out are The results printed in the usual and RESULTS pressENTER. ro cursor O PRINT the without graphs. format 8.6.8.Clearcard to the allows operator eraseall the on can not be erased its own.Thisfunction One result movethe cursor,oO CLEAR menu, on recorded the card.Fromthe MEMOCARD resutts Confirm the to is A ENTER. message displayed warntheuserabout erasing. andpress CARD ESC' or withENTER cancelwith for be re-used to is are The results erasedandthe backupnumber updated 0. The cardcan newresults.

AND TROUBLESHOOTING 9. MAINTENANCE

taaa

AND 9. MAINTENANCE TROUBLESHOOTING9.1.MAINTENANCE SERVICE AND 9.1.1. Overview results a well-maintained is to and factors contributing accurate reliable Oneof the principle properly. functioning The is to instrument. maintenancerequired keepyourinstrument Routine maintenance automated to a minimum. Thissection and ABC Vetis designed keepthis to procedures. maintenance describes dailyandperiodic the Dailymaintenance 9.1.2. performance yourABG procedures required dailyto maintain optimum of These are cleaning Vet

IMPORTANT

cleaningproceduresmay Failureto performany of these recommended result in decreasedreliabilityof your system.

cycles 9.1.2.1. andstandby Startup cyclemustbe performed. can be performed This At the beginning eachday,a startup of involvement manually pressing G'TD fey. or by tne without operator's automatically the goesinto"STANDBY" theend key.Theinstrument at At theendof eachday,press @ the (instrument in Leave instrument thismode the 1 overnight ofthecycle. Thiscycle takes minute. reagent visible thechambers. is in hasto beswitched inthismode), cleaner blue off 9.1.2.2. Automatic cleaning programmed the after by A cleaning automatically the number samples of cycleis activated to workload, section can see 8.3.4for operator. cyclefrequency be adjusted the laboratory The This fromtheservice menu(see theAUTOMATIC CLEANING SETUP. onecanbe rundirectly section 9.1.3.9). general cleaning 9.1.2.3. lnstrument with of Usewater anda dropof liquid Ingeneral, intrument to becleaned a wetpiece cloth. has Never solvant abrasive materials. of use or soapif necessary clean outside theinstrument. to the Disconnect instrument themain from electrical Wipe anytrace blood soonas possible. as of off is surethe instrument clean anddrybefore intervention make and supply before cleaning any reconnection. 9.1.2.4. filter Air nextto thepower in to at supply order An airfilteris placed inside instrument, therearbottom the or by retain dust.Thisfilterwillbe cleaned replaced an ABX DiagnosticsRepresentative the maintenance. Technician during preventive the Service only functions Service 9.1.3. These for functions available the userto cleanandcheckhis instrument. are Several service Move cursor the Function SERVICE the to functions accessible theMainmenu. from are l.T-) \-J : menu displayed is The andvalidate. SERVICE w,sttw - 4 W Iiwj,:1 hi

! . Wri: 1

.

,ffi

w

; - j

AND TROUBLESHOOTING 9. MAINTENANCEBackflush 9.1.3.1. Movethe in aperture caseof blockages. Thiscycleallows userto cleanthe instrument the is out ENTER. backflush The cycle carried and cursor thefunction BACKFLUSH press to O from are thatthe liquids aspirated the WBCand Check approximately. and lasts22 seconds backintothe thatthe liquids alsopushed are Check the RBCchambers through apertures. cleaning Perform concentrated a maybe blocked. the lf chambers. it is notthecase, aperture 9.1.3.4. procedure described section in as Drainchambers 9.1.3.2. properly to maintain and are thatthe chambers drained Thiscycleallows userto check the This it flushes liquid of the instrument. cycle out the manifold as somepartsof the hydraulic and CHAMBERS to Move tasts seconds 25 approximately. thecursor thefunction DRAIN [tl \___J pressENTER. - Checkthatthe wastechamber drained properly. not,checkthatthe wastelinefromthe lf is is or at chamber thewasteconnector the rearof the instrument notcrimped blocked. to - Checkthatthe WBCand RBCchambers drained properly. not,checkthatthe drain lf are or are chamber notcrimped blocked. to tubesgoing thewaste - Check properly. not,check tubesare lf thatthefollowing is chamber drained thatthe mixing : notcrimped blocked or - Check tubebetween bottom the mixing to chamber thetop of theWBCchamber. of the the - Check tubebetween topof theWBCchamber thevalve. to the the - Check tubebetween valve chamber. andthe waste the the Primereagents 9.1.3.3. containers one whenreplacing or all reagent the Thiscycleallows userto prime reagents the (orthepack). to Move cursor thefunction the S priming cycle: A - Bottlesand containersset - All reagents - Diluent priming - Lysepriming - Cleaner priming thattheyareclearof air bubbles. Check lines andpump. reagent Visually inspect B - ReagentPack given the and PACK follow instructions bytheLCDin order Select function : CHATGE the Q carried will cycle be automatically installed priming a Oncethe newPACK to install pack. the that linesandpump.Check theyareclearof airbubbles. reagent inspect out.Visually Run ENTER. therequired enfnfE andpress

AND TROUBLESHOOTING 9. MAINTENANCE9.1.3.4. Concentrated cleaning This of Thisprocedure cleaning the RBCand WBCapertures. cyclelasts3 allows strong a interventionfillthe with to chambers 1:5diluted the minutes approximately involves operator and bleach solution. :4 waterto parthigh 1 as deionized is The1:5bleach solution prepared follow parts NOTE: quality, - available (1 hypochlorite chloride). sodium bleach 0 -15% fragance-free ENTER. following The and CLEANING press Move cursor thefunction CONCENTRATED the to Q menuis displayed :

menuis displayed : anykey.Thefollowing coverdoorandpress Openthe instrument lN SOLIJTIONI POUR3MLOF BLEACH PRESS KEYTO CONTINUE;;". , A The into a pour chamber press key. and solution themixing Using 5mlsyringe, 3mlof bleach a : nextmenuis displayed IN4qBC CHAMBER SOLUTION POUR 3MLOF B-IEAOH ' ... PHESS KEYTO.CONTINUE A and pressa key.The next menuis inside the RBCchamber Pour3ml of bleach solution displayed:

menuis displayed : Thefollowing anykey. coverdoorandpress Close instrument the

Every10 seconds staris cleared The a off. of at Stars displayed the beginning thecycle. are to fromthe mixing chamber procedure of solution transfer thebleach involves cycles, different which cleaning theapertuof rinsing, allow perfect a aspiration, backflush, theWBCchamber, analysis begin. can sample is res.After thisprocedure completed, Transfer 9.1.3.5. chamber the WBCchamber. to Usinga fromthe MIXING It is possible check transfer the to Movethecursor thefunction chamber. to pour2mlof diluent the mixing into Smlsyringe, O menuis displayed the cycle starts: and The TRANSFER pressENTER. following and

P, in fromthe mixing chamber to for is check a blockage the lines lf thetransfer notcompleted, to fromthe WBCchamber the valve,andfromthe valveto the the WBCchamber, pressure/vacuum syringe.

AND TROUBLESHOOTING 9. MAINTENANCEchecks Mechanic 9.1.3.6. Move cursor thefunction to the O \__J : displayed is menu The ENTER. MECHANIC and MECHANIC press

of detection the allowthe userto checkthe correct A - Sensoroperations: Thisfunction and CHECXSENSORS press to Movethe cursor the function motorhomepositions. Q : is menu displayed SENSORS The ENTER. CHECK::1

needle - NEEDLE the front : SENSOR openthe instrument doorand pushupward sampling by : to on detection thedisplay0 turns 1 followed 10stars. thecorrect to support thetop.Check - CARRIAGE movethe sampling needle its upperposition, in : SENSOR withthe sampling to : on detection thedisplay 0 turns the Check correct handsideposition. on carriage theright 1 followed 10 stars. by

Press any keyto exitthefunction. rueeOf-E and U/D to : B - Needle anddownoperationMovethe cursor the function up Q and has the translation, movement to be smooth pressENTER. the observe needle Closely regular. UR NEEDLE andpress to : teft/right operationMovethe cursor the function C - Needle O and has the translation, movement to be smooth regular. the ENTER. observe needle Closely and SYRINGE pressENTER. to the : D - Liquid syringe Move cursor thefunction LIQUID O andregular. has the translation, movement to be smooth the Closely observe syringe SYRINGE to : syringe Movethe cursor the function E - pressure/vacuum fll eneSSURE has the translation, movement to be smooth the observe syringe Closely andpressENTER. andregular. observe Closely and to : the F - Valves Move cursor thefunction VALVES pressENTER. O and has the thevalveoperations, movement to be straight regular.

AND TROUBLESHOOTING 9. MAINTENANCEfor readability. the : can Move contrast be adjusted a better G - Change contrast Thedisplay cursor thefunction to O is displayed : The ENTER. CHGCONTRAST menu and CHGCONTRAST press

Press + keyof the numeric keyboard increase contrast, press - keyto reduce to the or the the pressENTER validthesetup. is Whenthe bestcontrast obtained, to thecontrast. NOTE I The contrast fromany othermenuby pressing menucan be accessed adjustment

ontn* simultaneously @

"nd O

t

"yr.

piston the upper position thedilution in H - Park: Raises vacuum/waste syringe and block the position. syringes the lower in 9.1.3.7. Gyclefunctions run Fromthe Thesefunctions allowthe userto checkthe cyclenumbers by its instrument. The to CVCIESand pressENTER. CYSERVICE menu, movethe cursor the function @ : CLESmenuis displayed

: ,3;f$$lli:i l;'t'r: : ,,,: Thefollowing STANDBY CBCs. and cycles be reviewedSTARTUP, can functions 9.1.3.8. Technician to on basis. Thisfunctions the engineer checkthe instrument a technical allows fieldservice password. Thesefunctions be accessed withthe useof a specific only can 9.1.3.9. Automatic cleaning Movethe cursor function to Q press"ENTER". anO

(seesection workload 8.3.4). Thecyclefrequency be ajdusted the laboratory to can

AND TROUBLESHOOTING 9. MAINTENANCE9.2.TROUBLESHOOTING 9.2.1. Overview if : require troubleshooting YourABC Vetmayoccasionally - System are operations faultY. - Thebackground high. is count unacceptably - Your (e.9., high results suspicious consistently are or values out-of-range, patient are control methods). results manual by to RBCcounts inability verify or - Precision poor. is - Calibration drifting. is see mentioned thefollowing in discussions, partsof theanalyzer to NOTE I lf needed, locate in 9.4. valveis listed section of 5. of thediagrams thesection Thefunction eachliquid procedure 9.2.2.ldentification malfunction: is the of session to identify source thesystem Thefirststepin anytroubleshooting out precision, calibration. Thesestepsshould carried in be or reagents, system operations, : below sequence described as . 9.2.2.1Systemoperations in 4. as operation described section lf the ABCVet the Press the keyandobserve instrument procedure. operations are lf properly, with continue the identification appears be operating to procedures appropriate troubleshooting and of faulty, identify source the malfunction initiate the (seesection 9.2.4). Reagents 9.2.2.2. your or values out-of-range,yourpatient are is lf yourbackground count unacceptable, control your Replace can or reagent deterioration contamination be suspected. results suspicious, are procedure Obtain in 9.1.3). described seciion cleaning the reagents perform concentrated and lf samples. reassay or controls patient 6.3),andif appropriate, count(seesection a background (or results are but countis acceptable, controlvalues stillout-of-range patient thebackground procedure. replacing reagents performing lf and with continue identification arestillsuspicious), count,call your ABX Diagnosfics does not correctthe background cleaning concentrated Representative SeruiceDepartment. 9.2.2.3. Precision (%CV). of the andcalculate coefficient variation 5 sample to 10times Analyze freshpatient a by of deviation the measurements the the by %CVis calculated dividing standard by this multiplying result 100. meanand

AND TROUBLESHOOTING 9. MAINTENANCE

X

'X

SD=

r(x-xi

where: X = mean n | - individual valueV.

deviation SD= standard Il = # of observations Parameters wBc RBC HGB HCT PLT %CV