Scientific Talks in general and Masterpiece Theatre in particular.
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Transcript of Scientific Talks in general and Masterpiece Theatre in particular.
![Page 1: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/1.jpg)
Scientific Talks
in general and
Masterpiece Theatre
in particular.
![Page 2: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/2.jpg)
The Ten Minute Rule
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Distill your data down to one point
Then state the significance in one sentence
Step 1 :
Isolate the Take Home Message (THM)
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Tarun Kapoor Examining mechanisms of cell division using real-time microscopy and chemical genetics
Mike Cox Genome restoration after radiation damage in Deinococcus radiodurans
Fall 2004 Speakers
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Step 2 The Introduction
Purpose- Start from nothing and get to the THM
Remember:
Who’s your Daddy?
Just the Facts
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•Determine the “main question” or hypothesis
•Very Specific
•The answer will have broad implications and be the THM
Remember:
Who’s your Daddy?
Just the Facts
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Introduction
(The question)
Conclusion
Results
(The data and interpretation)
(The THM )
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Q. How much data?
Q. How many slides?
A. = T/2(rule of thumb)
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The main question dictates the data you present
Data orSlide orOverhead
?YES
NO
Who’s your Daddy?
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Results Section
•Longest section = .7 T•Logical flow•Avoid vague statements•Draw conclusions (different from THM)
Conclusion:
Mutations in abc1 cause abnormal eye development
THM:
Eye development is regulated by ubiquitylation
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Know Your Data
•Practice without it
•What the data are•What the data mean•Where the data lead
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Step 3 The Conclusion
•Recap the question
•Recap the most important conclusion(s)
•State the THM
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The Three Tellums
1. Tellum what you’re gonna tellum
2. Then tellum
3. Then tellum what you toldum
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Slides - audiovisual aids
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Tor signaling
•Tor is a highly conserved PI3 (lipid) kinase that is a protein kinase in vitro, and presumably in vivo.•So-called because it is the “target of rapamycin”•Rapamycin is a drug that was identified from the soil of the island of “Rapa Nui” (also known as Easter Island) in a screen for anti-fungal compounds•Yeast mutants resistant to rapamycin identified three genes TOR1 TOR2 and FRP1•FRP1 encodes a homolog of FK-506 binding protein•It is rapamycin bound to FK-506 binding protein that binds to and inhibits the Tor1 and Tor2 protein kinases
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YEPD YEPD + Ben
Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2
A
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
B
C WCE IgG IP Sup-IgG IP
Mad3-PrA
Mad2
Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +
1 2 3 4 5 6 7 8 9
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
D
![Page 19: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/19.jpg)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
![Page 20: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/20.jpg)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
![Page 21: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/21.jpg)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
![Page 22: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/22.jpg)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
![Page 23: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/23.jpg)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
![Page 24: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/24.jpg)
YEPD YEPD + Ben
Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2
A
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
B
C WCE IgG IP Sup-IgG IP
Mad3-PrA
Mad2
Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +
1 2 3 4 5 6 7 8 9
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
D
![Page 25: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/25.jpg)
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
![Page 26: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/26.jpg)
Pull down Mad3-PrA
(Blot Mad2)
WCE
SUP
Pulldown Cdc20-13Myc
Mad3
PrA
Mad2 Mad2
Cdc20
Myc
(Blot Mad2)
Sequential Immunoprecipitation
![Page 27: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/27.jpg)
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
![Page 28: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/28.jpg)
Mad3
PrA
Mad2 Mad2
Cdc20
Myc
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
![Page 29: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/29.jpg)
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad2
Cdc20
Myc
Mad3
PrA
Mad2
![Page 30: Scientific Talks in general and Masterpiece Theatre in particular.](https://reader035.fdocuments.us/reader035/viewer/2022062423/5697bf871a28abf838c88751/html5/thumbnails/30.jpg)
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad2
Cdc20
Myc
Mad3
PrA
Mad2
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“Every product of genius is a product of enthusiasm”
Benjamin Disreali
The Delivery
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And finally…………..
Dress for Success
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The Ten Minute Rule
No matter what the talk/seminar is:
T/2 slides
Be professional