8/2/2019 sb bld 430 quiz 9 key

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BLD 430 Quiz 9

1. Realtime PCR uses polymerase enzyme.A) True

B) False

2. Realtime PCR rarely uses a gel.

A) True

B) False

3. Realtime PCR can use probes with fluorchrome labels.A) True

B) False

4. One probe scenario in realtime PCR is to have two dyes on the same probe but if the

probe is intact, one fluorochrome can quench the second and therefore no signal is

generated.A) True

B) False

5. One probe scenario in realtime PCR is to have two dyes where one quenches the other

but if unbound, there is secondary structure that keeps them close enough that there is the

quenching phenomena but if bound, they are physically separated enough to allow signaldetection.

A) True

B) False

6.One probe scenario in realtime pcr is two have two dyes labeling a probe that when theprobe is intact, the two dyes are close enough that when one is excited it emits at a

wavelength that can excite the second generating a signal.

A) True

B) False

7. Realtime PCR can be quantitative if a standard curve is generated with each run.A) True

B) False

8. Realtime PCR can be used for genotyping by using probes specific for each

polymorphism that have separate fluorochromes.A) True

B) False

9. Realtime PCR can be used for genotyping if melting curve analysis is validated for the

expected polymorphisms.A) True

8/2/2019 sb bld 430 quiz 9 key

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B) False

10. Realtime PCR can be used for genotyping and in the same assay, homozygous andheterozygous individuals can be identified.

A) True

B) False