8/2/2019 sb bld 430 quiz 8 key

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BLD 430 Quiz 8 Key

1. The polymerase chain reaction requires that you have high molecular weight, very pureDNA to ensure success of the assay compared to an RFLP based assay.

A) True

B) False

2. The PCR reaction is dependent upon the Ca++ concentration in the reaction.

A) True

B) False

3. SSO and ARMS are the same format of testing.

A) True

B) False

4. A dot/blot and a blot/dot are equivalent in principle, it just depends on whether the

probes or the target DNA are immobilized.A) TrueB) False

5. It most important the the 5' base of a primer is bound to allow the polymerase enzyme

to react.A) True

B) False

6. Touchdown PCR involves the use a polymerase for E. coli rather than T. aquaticusA) True

B) False

7. Most reagents in the PCR reaction mix are added in excess to allow the reaction to be

as robust as possible.

A) True

B) False

8. The specificity of a PCR reaction can be manipulated by the sequence of the primers

used and/or the temperature of annealing set in the protocol.

A) True

B) False

9. The sensitivity of the PCR reaction is set by controlling the temperature of annealing

and/or number of cycles

A) True

B) False

10. Any component of the PCR reaction mixture and/or manipulation of cycle numbers or

temperature parameters will affect the fidelity of the amplification reaction.

8/2/2019 sb bld 430 quiz 8 key

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A) True

B) False