Salk Institute Mobile Lab Gel Electrophoresis Student Instructions Edvotek set up Salk Institute...
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Transcript of Salk Institute Mobile Lab Gel Electrophoresis Student Instructions Edvotek set up Salk Institute...
Salk Institute Mobile Lab
Gel Electrophoresis
Student Instructions
Edvotek set up
Salk Institute Mobile Lab
Salk Institute Mobile Lab
Gel Electrophoresis Student Station Set-up
tip container w/tips
waste container
5µl fixed pipettor
practice sheet in protector
foam epi rack w/ 1 food color tube each: orange, green, purple
Salk Institute Mobile Lab
How to Use a 5µl fixed pipettor
• Make sure a yellow tip is firmly twisted onto the end of the red pipettor
• Carefully open one of the food color tubes – Don’t let the lid snap back
and splash you!
• Hold down the button on the top of the pipettor
Salk Institute Mobile Lab
How to Use a 5µl fixed pipettor
• While holding the button down, insert the tip into the food color tube
Salk Institute Mobile Lab
How to Use a 5µl fixed pipettor
• Release the button and remove from the food color tube
• The tip now contains just 5 microliters of solution!
Salk Institute Mobile Lab
How to Use a 5µl fixed pipettor
• To dispense the solution push down on the button again
• Using your other hand to guide the end of the tip, practice dispensing the 5µl drops – Try to not push down on
the plastic sheet - see if you can hold the pipettor tip just above the paper
Salk Institute Mobile Lab
How to Use a 5µl fixed pipettor
• Twist off the dirty tip into the waste container and replace with a clean tip
• Always use a clean tip each time!!
Salk Institute Mobile Lab
How to Use a 5µl fixed pipettor
• Ready to load a real gel?
Salk Institute Mobile Lab
How to Use a 5µl fixed pipettor
• Load the food color solutions into the wells on your group’s gel
• Be careful not to poke through the bottom of the wells!