Sajib Intro
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PHYTOCHEMICAL AND IN-VITRO STUDY OF ETHYL ACETATE EXTRACT OF
Spondias pinnata
A thesis report, submitted to the Department of Pharmacy, East West University, in partial
fulfillment of the requirements of the Degree of Pharmacy
Submitted by
Md. Nasidul Islam
ID 2008-3-70-098
Research Supervisor
Kh. Tanvir Ahmed
Senior Lecturer
Department of Pharmacy
East West University
Aftabnagor, Rampura, Dhaka.
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This Research Paper is dedicated to
Almighty ALLAH
The most Gracious
The most Merciful
&
To My Parents
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Declaration by the Research Candidate
I, Md. Nasidul Islam, hereby declare that the dissertation entitledPhytochemical and in-vitrostudy of methanolic extract of Spondias pinnata, submitted by me to the Department of
Pharmacy, East West University, Bangladesh, in the partial fulfillment of the requirement for the
award of the degree of Bachelor of Pharmacy is a bonafide record of original research work carried
out by me in 2012 under the supervision and guidance of Kh. Tanvir Ahmed, Senior lecturer,
Department of Pharmacy, East West University and it has not formed the basis for the award of any
other Degree/Diploma/Fellowship or other similar title to any candidate of any University.
Place
Dhaka
Signature of the Candidate
Md. Nasidul Islam
_______________________
Date: 23/ 12 / 2012 ID# 2008-3-70-098
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Certificate
This is to certify that the thesis submitted to the Department of Pharmacy, East West University,Bangladesh, in partial fulfillment of the requirement for the Degree of Bachelor in Pharmacy, was
carried out by Md. Nasidul Islam ID. 2008-3-70-098.
Sufia Islam, Ph.D
Chairperson and Associate Professor
Department of Pharmacy
East West University
Aftabnagor, Rampura, Dhaka
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Certificate
This is to certify that the thesis Phytochemical and in-vitro study of methanolic extract ofSpondias pinnata submitted to the Department of Pharmacy, East West University, Bangladesh, in
partial fulfillment of the requirement for the degree of Bachelor of Pharmacy was carried out by Md.
Nasidul Islam ID. 2008-3-70-098,under my guidance and supervision and that no part of the thesis
has been submitted for any other degree. I further certify that all the sources of information,
laboratory facilities availed of this connection is dully acknowledged.
Kh. Tanvir Ahmed
Senior lecturer
Department of Pharmacy
East West University
Aftabnagor, Rampura, Dhaka
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Acknowledgement
First, all praise and glory are to Allah for all the bounties granted to me and only with His
guidance and help this achievement has become possible.
I wish to express my thanks and gratitude towards my supervisorKh. Tanvir Ahmed, Senior
Lecturer, Department of Pharmacy, East West University, for his constant encouragement,
helpful discussions and able guidance throughout this study. His generosity with time and
constructive comments were of vital support and were above the call of duty.
I would also like to specially thankMd. Amran Howlader, Senior Lecturer, Department of
Pharmacy; Apurba Sarkar Apu, Senior Lecturer, Department of Pharmacy, East West
University for the continuous support. Their advice and instructions helped me to get the best
results.
I am indebted to Dr. Sufia Islam, Chairperson and Associate Professor, East West University,
for her kind support and the permission to use the facilities available at the Department of
Pharmacy, East West University, as well as for her encouragement to carry out research.
I would like to convey deepest love and obedience to my caring parents and elder sister fortheir support and guiding me all through my life until today, which keeps me strong and honest
to do the things I needed to do.
Finally I would also like to thank all the students especially Mohiuddin, Md.
Shamimuzzaman, Md. Mahfuz-e alam, A. K. M. Radowan Ahmad, Md. Mustakim Billah,
Md. Aminul Islam, Keya, Farzana Haq, Naifa Akhter, Tasnuba Karim, Laboni, Sumaya,
Tamanna for their technical direction and co-operation which enabled me to work in a very
congenial and comfortable atmosphere.
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AbstractSpondias pinnata is an evergreen parasitic plant under the family anacardiaceae and used
traditionally in medicinal system. This plant reported to have in vitro antioxidant activity
(non-enzymatic hemoglobin glycosylation), and antibacterial activity. The intention of the
study was to emphasize on the separating the compounds of methanol extracts of S.pinnata
according to their polarity and evaluate their cytotoxicity, antioxidant activity and
antimicrobial activity. To fulfill this purpose I did partition of the extracts of S.pinnata into
Ethyl acetate solvent system. Then evaluate the antioxidant activity , cytotoxic property by
using brine shrimp lethality bioassay. After collection, identification and drying the tree
sample, extraction was performed by using methanol solvent system. Then partition was
done. Then extracted components were used to evaluation.
Key Words:Spondias pinnata, Brine shrimp lethality assay, Ethyl acetate
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List of Contents
CHAPTER 1: INTRODUCTION.1-17
CHAPTER 2: LITERATURE REVIEW ....18-23
CHAPTER 3: PLANT DESCRIPTION.....24-34
CHAPTER 4: COLLENTION, DRYING AND EXTRACTION...35-38
CHAPTER 5: DESIGN OF IN-VITROTEST.......................................................39-41
CHAPTER 6: METHODS AND MATERIALS.42-66
CHAPTER 7: RESULTS AND DISCUSSIONS...67-79
Conclusion.......80
References.....81-83