SAFETY DATA SHEET · First-Strand cDNA Synthesis Kit for Real-Time PCR Section 2. Hazards...
Transcript of SAFETY DATA SHEET · First-Strand cDNA Synthesis Kit for Real-Time PCR Section 2. Hazards...
First-Strand cDNA Synthesis Kit for Real-Time PCR
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SAFETY DATA SHEET
GHS product identifier
Other means of identification
Section 1. Identification:
:
First-Strand cDNA Synthesis Kit for Real-Time PCR
3420 Central Expressway, Santa Clara CA 95051Supplier/Manufacturer
In case of emergency :
:
Chemtrec: 1 800 424 9300Outside USA & Canada: +1 703 527 3887
75780Code :
Section 2. Hazards identificationOSHA/HCS status : 10X RT Buffer This material is considered hazardous by the OSHA
Hazard Communication Standard (29 CFR 1910.1200).PCR Nucleotide Mix, 10 mM Solution
While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.
RNase Inhibitor This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200).
Water, RNase-Free, DEPC Treated
While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.
MMLV-RT This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200).
50 µM Anchored Oligo dT23VN
While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.
75 µM Random Hexamer While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.
10X Primer Mix While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.
0.1 mg/ml HeLa RNA While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.
Control Primer Mix for qPCR While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.
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Section 2. Hazards identification
10X RT Buffer SERIOUS EYE DAMAGE/ EYE IRRITATION - Category 2BSKIN SENSITIZATION - Category 1
PCR Nucleotide Mix, 10 mM Solution
Not classified.
RNase Inhibitor SERIOUS EYE DAMAGE/ EYE IRRITATION - Category 2B
Water, RNase-Free, DEPC Treated
Not classified.
MMLV-RT SERIOUS EYE DAMAGE/ EYE IRRITATION - Category 2B
50 µM Anchored Oligo dT23VN
Not classified.
75 µM Random Hexamer Not classified.10X Primer Mix Not classified.0.1 mg/ml HeLa RNA Not classified.Control Primer Mix for qPCR Not classified.
Classification of the substance or mixture
:
Signal word : 10X RT Buffer WarningPCR Nucleotide Mix, 10 mM Solution
No signal word.
RNase Inhibitor WarningWater, RNase-Free, DEPC Treated
No signal word.
MMLV-RT Warning50 µM Anchored Oligo dT23VN
No signal word.
75 µM Random Hexamer No signal word.10X Primer Mix No signal word.0.1 mg/ml HeLa RNA No signal word.Control Primer Mix for qPCR No signal word.
Hazard statements : 10X RT Buffer Causes eye irritation.May cause an allergic skin reaction.
PCR Nucleotide Mix, 10 mM Solution
No known significant effects or critical hazards.
RNase Inhibitor Causes eye irritation.Water, RNase-Free, DEPC Treated
No known significant effects or critical hazards.
MMLV-RT Causes eye irritation.50 µM Anchored Oligo dT23VN
No known significant effects or critical hazards.
75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.
Hazard pictograms :
Precautionary statements
GHS label elements
1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.
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Section 2. Hazards identification
Prevention : 10X RT Buffer Wear protective gloves. Wear eye or face protection.Avoid breathing vapor. Wash hands thoroughly after handling. Contaminated work clothing should not be allowed out of the workplace.
PCR Nucleotide Mix, 10 mM Solution
Not applicable.
RNase Inhibitor Wear eye or face protection. Wash hands thoroughly after handling.
Water, RNase-Free, DEPC Treated
Not applicable.
MMLV-RT Wear eye or face protection. Wash hands thoroughly after handling.
50 µM Anchored Oligo dT23VN
Not applicable.
75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.
Response : 10X RT Buffer IF ON SKIN: Wash with plenty of soap and water.Wash contaminated clothing before reuse. If skin irritation or rash occurs: Get medical attention. IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do.Continue rinsing. If eye irritation persists: Get medical attention.
PCR Nucleotide Mix, 10 mM Solution
Not applicable.
RNase Inhibitor IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical attention.
Water, RNase-Free, DEPC Treated
Not applicable.
MMLV-RT IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical attention.
50 µM Anchored Oligo dT23VN
Not applicable.
75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.
General : 10X RT Buffer Not applicable.PCR Nucleotide Mix, 10 mM Solution
Not applicable.
RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated
Not applicable.
MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN
Not applicable.
75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.
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Section 2. Hazards identificationStorage : 10X RT Buffer Not applicable.
PCR Nucleotide Mix, 10 mM Solution
Not applicable.
RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated
Not applicable.
MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN
Not applicable.
75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.
Disposal : 10X RT Buffer Dispose of contents and container in accordance with all local, regional, national and international regulations.
PCR Nucleotide Mix, 10 mM Solution
Not applicable.
RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated
Not applicable.
MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN
Not applicable.
75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.
Supplemental label elements
: 10X RT Buffer None known.PCR Nucleotide Mix, 10 mM Solution
None known.
RNase Inhibitor None known.Water, RNase-Free, DEPC Treated
None known.
MMLV-RT None known.50 µM Anchored Oligo dT23VN
None known.
75 µM Random Hexamer None known.10X Primer Mix None known.0.1 mg/ml HeLa RNA None known.Control Primer Mix for qPCR None known.
Hazards not otherwise classified
: 10X RT Buffer None known.PCR Nucleotide Mix, 10 mM Solution
None known.
RNase Inhibitor None known.Water, RNase-Free, DEPC Treated
None known.
MMLV-RT None known.50 µM Anchored Oligo dT23VN
None known.
75 µM Random Hexamer None known.10X Primer Mix None known.0.1 mg/ml HeLa RNA None known.Control Primer Mix for qPCR None known.
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Section 3. Composition/information on ingredients
10X RT Bufferpotassium chloride 1 - 10 7447-40-72-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride 1 - 10 1185-53-1trometamol 1 - 10 77-86-1(R*,R*)-1,4-dimercaptobutane-2,3-diol 0 - 1 3483-12-3
RNase Inhibitorglycerol 25 - 50 56-81-5
MMLV-RTglycerol 25 - 50 56-81-5
Ingredient name CAS number%
There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section.
Substance/mixture :
Occupational exposure limits, if available, are listed in Section 8.
Mixture
Any concentration shown as a range is to protect confidentiality or is due to batch variation.
10X RT Buffer Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Continue to rinse for at least 10 minutes. If irritation persists, get medical attention.
PCR Nucleotide Mix, 10 mM Solution
Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.
RNase Inhibitor Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Continue to rinse for at least 10 minutes. If irritation persists, get medical attention.
Water, RNase-Free, DEPC Treated
Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.
MMLV-RT Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Continue to rinse for at least 10 minutes. If irritation persists, get medical attention.
50 µM Anchored Oligo dT23VN
Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.
75 µM Random Hexamer Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.
10X Primer Mix Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check
Section 4. First aid measures
Eye contact :
Description of necessary first aid measures
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Section 4. First aid measuresfor and remove any contact lenses. Get medical attention if irritation occurs.
0.1 mg/ml HeLa RNA Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.
Control Primer Mix for qPCR Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.
10X RT Buffer Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention if adverse health effects persist or are severe. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband. In case of inhalation of decomposition products in a fire, symptoms may be delayed. The exposed person may need to be kept under medical surveillance for 48 hours.
PCR Nucleotide Mix, 10 mM Solution
Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.
RNase Inhibitor Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention if adverse health effects persist or are severe. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.
Water, RNase-Free, DEPC Treated
Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.
MMLV-RT Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention if adverse health effects persist or are severe. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.
50 µM Anchored Oligo dT23VN
Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.
75 µM Random Hexamer Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.
10X Primer Mix Remove victim to fresh air and keep at rest in a position
Inhalation :
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Section 4. First aid measures
10X RT Buffer Wash out mouth with water. Remove dentures if any.Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention if adverse health effects persist or are severe.Never give anything by mouth to an unconscious person.If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.
10X RT Buffer Wash with plenty of soap and water. Remove contaminated clothing and shoes. Wash contaminated clothing thoroughly with water before removing it, or wear gloves. Continue to rinse for at least 10 minutes.Get medical attention. In the event of any complaints or symptoms, avoid further exposure. Wash clothing before reuse. Clean shoes thoroughly before reuse.
PCR Nucleotide Mix, 10 mM Solution
Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.
RNase Inhibitor Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur. Wash clothing before reuse. Clean shoes thoroughly before reuse.
Water, RNase-Free, DEPC Treated
Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.
MMLV-RT Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur. Wash clothing before reuse. Clean shoes thoroughly before reuse.
50 µM Anchored Oligo dT23VN
Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.
75 µM Random Hexamer Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.
10X Primer Mix Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.
0.1 mg/ml HeLa RNA Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.
Control Primer Mix for qPCR Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.
comfortable for breathing. Get medical attention if symptoms occur.
0.1 mg/ml HeLa RNA Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.
Control Primer Mix for qPCR Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.
Skin contact
Ingestion :
:
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Section 4. First aid measuresPCR Nucleotide Mix, 10 mM Solution
Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.
RNase Inhibitor Wash out mouth with water. Remove dentures if any.Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention if adverse health effects persist or are severe.Never give anything by mouth to an unconscious person.If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.
Water, RNase-Free, DEPC Treated
Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.
MMLV-RT Wash out mouth with water. Remove dentures if any.Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention if adverse health effects persist or are severe.Never give anything by mouth to an unconscious person.If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.
50 µM Anchored Oligo dT23VN
Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.
75 µM Random Hexamer Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.
10X Primer Mix Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical
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Section 4. First aid measurespersonnel. Get medical attention if symptoms occur.
0.1 mg/ml HeLa RNA Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.
Control Primer Mix for qPCR Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.
Most important symptoms/effects, acute and delayed
Inhalation : 10X RT Buffer Exposure to decomposition products may cause a health hazard. Serious effects may be delayed following exposure.
PCR Nucleotide Mix, 10 mM Solution
No known significant effects or critical hazards.
RNase Inhibitor No known significant effects or critical hazards.Water, RNase-Free, DEPC Treated
No known significant effects or critical hazards.
MMLV-RT No known significant effects or critical hazards.50 µM Anchored Oligo dT23VN
No known significant effects or critical hazards.
75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.
Skin contact : 10X RT Buffer May cause an allergic skin reaction.PCR Nucleotide Mix, 10 mM Solution
No known significant effects or critical hazards.
RNase Inhibitor No known significant effects or critical hazards.Water, RNase-Free, DEPC Treated
No known significant effects or critical hazards.
MMLV-RT No known significant effects or critical hazards.50 µM Anchored Oligo dT23VN
No known significant effects or critical hazards.
75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.
10X RT Buffer Causes eye irritation.PCR Nucleotide Mix, 10 mM Solution
No known significant effects or critical hazards.
RNase Inhibitor Causes eye irritation.Water, RNase-Free, DEPC Treated
No known significant effects or critical hazards.
MMLV-RT Causes eye irritation.50 µM Anchored Oligo dT23VN
No known significant effects or critical hazards.
75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.
:Eye contact
Potential acute health effects
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Section 4. First aid measures10X RT Buffer May be irritating to mouth, throat and stomach.PCR Nucleotide Mix, 10 mM Solution
No known significant effects or critical hazards.
RNase Inhibitor May be irritating to mouth, throat and stomach.Water, RNase-Free, DEPC Treated
No known significant effects or critical hazards.
MMLV-RT May be irritating to mouth, throat and stomach.50 µM Anchored Oligo dT23VN
No known significant effects or critical hazards.
75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.
:Ingestion
Over-exposure signs/symptoms
Skin contact
Inhalation 10X RT Buffer No specific data.PCR Nucleotide Mix, 10 mM Solution
No specific data.
RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated
No specific data.
MMLV-RT No specific data.50 µM Anchored Oligo dT23VN
No specific data.
75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.
10X RT Buffer Adverse symptoms may include the following:irritationredness
PCR Nucleotide Mix, 10 mM Solution
No specific data.
RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated
No specific data.
MMLV-RT No specific data.
:
:
Eye contact : 10X RT Buffer Adverse symptoms may include the following:irritationwateringredness
PCR Nucleotide Mix, 10 mM Solution
No specific data.
RNase Inhibitor Adverse symptoms may include the following:irritationwateringredness
Water, RNase-Free, DEPC Treated
No specific data.
MMLV-RT Adverse symptoms may include the following:irritationwateringredness
50 µM Anchored Oligo dT23VN
No specific data.
75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.
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Section 4. First aid measures
Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training.
Notes to physician : Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled.
Specific treatments : No specific treatment.
Ingestion 10X RT Buffer No specific data.PCR Nucleotide Mix, 10 mM Solution
No specific data.
RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated
No specific data.
MMLV-RT No specific data.50 µM Anchored Oligo dT23VN
No specific data.
75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.
50 µM Anchored Oligo dT23VN
No specific data.
75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.
:
See toxicological information (Section 11)
Indication of immediate medical attention and special treatment needed, if necessary
Section 5. Fire-fighting measures
10X RT Buffer Use an extinguishing agent suitable for the surrounding fire.
PCR Nucleotide Mix, 10 mM Solution
Use an extinguishing agent suitable for the surrounding fire.
RNase Inhibitor Use an extinguishing agent suitable for the surrounding fire.
Water, RNase-Free, DEPC Treated
Use an extinguishing agent suitable for the surrounding fire.
MMLV-RT Use an extinguishing agent suitable for the surrounding fire.
50 µM Anchored Oligo dT23VN
Use an extinguishing agent suitable for the surrounding fire.
75 µM Random Hexamer Use an extinguishing agent suitable for the surrounding fire.
10X Primer Mix Use an extinguishing agent suitable for the surrounding fire.
0.1 mg/ml HeLa RNA Use an extinguishing agent suitable for the surrounding fire.
Control Primer Mix for qPCR Use an extinguishing agent suitable for the surrounding fire.
Extinguishing media
Suitable extinguishing media
:
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Section 5. Fire-fighting measures
Hazardous thermal decomposition products
Specific hazards arising from the chemical
10X RT Buffer Decomposition products may include the following materials:carbon dioxidecarbon monoxidenitrogen oxideshalogenated compoundsmetal oxide/oxides
PCR Nucleotide Mix, 10 mM Solution
No specific data.
RNase Inhibitor Decomposition products may include the following materials:carbon dioxidecarbon monoxide
Water, RNase-Free, DEPC Treated
No specific data.
MMLV-RT Decomposition products may include the following materials:carbon dioxidecarbon monoxide
50 µM Anchored Oligo dT23VN
No specific data.
75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.
10X RT Buffer In a fire or if heated, a pressure increase will occur and the container may burst.
PCR Nucleotide Mix, 10 mM Solution
In a fire or if heated, a pressure increase will occur and the container may burst.
RNase Inhibitor In a fire or if heated, a pressure increase will occur and the container may burst.
Water, RNase-Free, DEPC Treated
In a fire or if heated, a pressure increase will occur and the container may burst.
MMLV-RT In a fire or if heated, a pressure increase will occur and the container may burst.
50 µM Anchored Oligo dT23VN
In a fire or if heated, a pressure increase will occur and the container may burst.
75 µM Random Hexamer In a fire or if heated, a pressure increase will occur and the container may burst.
10X Primer Mix In a fire or if heated, a pressure increase will occur and the container may burst.
0.1 mg/ml HeLa RNA In a fire or if heated, a pressure increase will occur and the container may burst.
Control Primer Mix for qPCR In a fire or if heated, a pressure increase will occur and the container may burst.
:
:
10X RT Buffer None known.PCR Nucleotide Mix, 10 mM Solution
None known.
RNase Inhibitor None known.Water, RNase-Free, DEPC Treated
None known.
MMLV-RT None known.50 µM Anchored Oligo dT23VN
None known.
75 µM Random Hexamer None known.10X Primer Mix None known.0.1 mg/ml HeLa RNA None known.Control Primer Mix for qPCR None known.
Unsuitable extinguishing media
:
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Section 5. Fire-fighting measures
10X RT Buffer Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
PCR Nucleotide Mix, 10 mM Solution
Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
RNase Inhibitor Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
Water, RNase-Free, DEPC Treated
Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
MMLV-RT Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
50 µM Anchored Oligo dT23VN
Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
75 µM Random Hexamer Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
10X Primer Mix Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
0.1 mg/ml HeLa RNA Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
Control Primer Mix for qPCR Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.
Control Primer Mix for qPCR No specific data.
10X RT Buffer Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
PCR Nucleotide Mix, 10 mM Solution
Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
RNase Inhibitor Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
Water, RNase-Free, DEPC Treated
Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
MMLV-RT Fire-fighters should wear appropriate protective
Special protective equipment for fire-fighters
:
Special protective actions for fire-fighters
:
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Section 5. Fire-fighting measuresequipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
50 µM Anchored Oligo dT23VN
Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
75 µM Random Hexamer Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
10X Primer Mix Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
0.1 mg/ml HeLa RNA Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
Control Primer Mix for qPCR Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.
Section 6. Accidental release measures
Environmental precautions
Personal precautions, protective equipment and emergency procedures
Stop leak if without risk. Move containers from spill area. Prevent entry into sewers,water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible,absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see Section 13). Dispose of via a licensed waste disposal contractor. Note: see Section 1 for emergency contact information and Section 13 for waste disposal.
:
: No action shall be taken involving any personal risk or without suitable training.Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Put on appropriate personal protective equipment.
Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air).
Large spill :
Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor.
Small spill :
Methods and materials for containment and cleaning up
For non-emergency personnel
For emergency responders : If specialised clothing is required to deal with the spillage, take note of any information in Section 8 on suitable and unsuitable materials. See also the information in "For non-emergency personnel".
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 7. Handling and storage
Advice on general occupational hygiene
10X RT Buffer Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
PCR Nucleotide Mix, 10 mM Solution
Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
RNase Inhibitor Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before
:
Protective measures 10X RT Buffer Put on appropriate personal protective equipment (see Section 8). Persons with a history of skin sensitization problems should not be employed in any process in which this product is used. Do not get in eyes or on skin or clothing. Do not ingest. Avoid breathing vapor or mist. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container.
PCR Nucleotide Mix, 10 mM Solution
Put on appropriate personal protective equipment (see Section 8).
RNase Inhibitor Put on appropriate personal protective equipment (see Section 8). Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container.
Water, RNase-Free, DEPC Treated
Put on appropriate personal protective equipment (see Section 8).
MMLV-RT Put on appropriate personal protective equipment (see Section 8). Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container.
50 µM Anchored Oligo dT23VN
Put on appropriate personal protective equipment (see Section 8).
75 µM Random Hexamer Put on appropriate personal protective equipment (see Section 8).
10X Primer Mix Put on appropriate personal protective equipment (see Section 8).
0.1 mg/ml HeLa RNA Put on appropriate personal protective equipment (see Section 8).
Control Primer Mix for qPCR Put on appropriate personal protective equipment (see Section 8).
:
Precautions for safe handling
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 7. Handling and storageentering eating areas. See also Section 8 for additional information on hygiene measures.
Water, RNase-Free, DEPC Treated
Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
MMLV-RT Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
50 µM Anchored Oligo dT23VN
Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
75 µM Random Hexamer Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
10X Primer Mix Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
0.1 mg/ml HeLa RNA Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
Control Primer Mix for qPCR Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 7. Handling and storageConditions for safe storage,including any incompatibilities
10X RT Buffer Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
PCR Nucleotide Mix, 10 mM Solution
Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
RNase Inhibitor Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
Water, RNase-Free, DEPC Treated
Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
MMLV-RT Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
50 µM Anchored Oligo dT23VN
Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
75 µM Random Hexamer Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.
:
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 7. Handling and storageContainers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
10X Primer Mix Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
0.1 mg/ml HeLa RNA Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
Control Primer Mix for qPCR Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.
RNase Inhibitorglycerol OSHA PEL 1989 (United States, 3/1989).
TWA: 5 mg/m³ 8 hours. Form: Respirable fraction TWA: 10 mg/m³ 8 hours. Form: Total dustOSHA PEL (United States, 2/2013). TWA: 5 mg/m³ 8 hours. Form: Respirable fraction TWA: 15 mg/m³ 8 hours. Form: Total dust
MMLV-RTglycerol OSHA PEL 1989 (United States, 3/1989).
TWA: 5 mg/m³ 8 hours. Form: Respirable fraction TWA: 10 mg/m³ 8 hours. Form: Total dustOSHA PEL (United States, 2/2013). TWA: 5 mg/m³ 8 hours. Form: Respirable fraction TWA: 15 mg/m³ 8 hours. Form: Total dust
Section 8. Exposure controls/personal protection
Ingredient name Exposure limits
Control parameters
Occupational exposure limits
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 8. Exposure controls/personal protection
Hand protection
Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator.
Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary.
Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists,gases or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: safety glasses with side-shields.
Eye/face protection
Respiratory protection :
:
:
Body protection Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product.
:
Environmental exposure controls
: Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels.
Appropriate engineering controls
: Good general ventilation should be sufficient to control worker exposure to airborne contaminants.
Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period.Appropriate techniques should be used to remove potentially contaminated clothing.Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location.
Hygiene measures :
Individual protection measures
Skin protection
Other skin protection : Appropriate footwear and any additional skin protection measures should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product.
Section 9. Physical and chemical properties10X RT Buffer Liquid.PCR Nucleotide Mix, 10 mM Solution
Liquid.
RNase Inhibitor Liquid.Water, RNase-Free, DEPC Treated
Liquid.
MMLV-RT Liquid.50 µM Anchored Oligo dT23VN
Liquid.
75 µM Random Hexamer Liquid.10X Primer Mix Liquid.0.1 mg/ml HeLa RNA Liquid.Control Primer Mix for qPCR Liquid.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
Physical state
Color
:
:
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 9. Physical and chemical properties
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
Odor :
Flash point : 10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
:Auto-ignition temperature
Flammable limits : 10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 9. Physical and chemical properties
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
100°C (212°F)
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not applicable.PCR Nucleotide Mix, 10 mM Solution
Not applicable.
RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated
18.02 g/mole
MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN
Not applicable.
75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.
Boiling/condensation point
Molecular weight
pH
10X RT Buffer Not applicable.PCR Nucleotide Mix, 10 mM Solution
Not applicable.
RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated
H2-O
MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN
Not applicable.
75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.
Molecular formula
:
:
:
:
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 9. Physical and chemical properties10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
0°C (32°F)
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
3.2 kPa (23.8 mm Hg) [room temperature]
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
Melting/freezing point
Relative density
Vapor pressure
Vapor density
:
:
:
:
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 9. Physical and chemical properties10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.water Not available.MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.
Volatility
Evaporation rate
Viscosity
Solubility
Physical/chemical properties comments
:
:
:
:
:
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 9. Physical and chemical properties50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.
Section 10. Stability and reactivity
10X RT Buffer The product is stable.PCR Nucleotide Mix, 10 mM Solution
The product is stable.
RNase Inhibitor The product is stable.Water, RNase-Free, DEPC Treated
The product is stable.
MMLV-RT The product is stable.50 µM Anchored Oligo dT23VN
The product is stable.
75 µM Random Hexamer The product is stable.10X Primer Mix The product is stable.0.1 mg/ml HeLa RNA The product is stable.Control Primer Mix for qPCR The product is stable.
Chemical stability :
Possibility of hazardous reactions
: 10X RT Buffer Under normal conditions of storage and use, hazardous reactions will not occur.
PCR Nucleotide Mix, 10 mM Solution
Under normal conditions of storage and use, hazardous reactions will not occur.
RNase Inhibitor Under normal conditions of storage and use, hazardous reactions will not occur.
Water, RNase-Free, DEPC Treated
Under normal conditions of storage and use, hazardous reactions will not occur.
MMLV-RT Under normal conditions of storage and use, hazardous reactions will not occur.
50 µM Anchored Oligo dT23VN
Under normal conditions of storage and use, hazardous reactions will not occur.
75 µM Random Hexamer Under normal conditions of storage and use, hazardous reactions will not occur.
10X Primer Mix Under normal conditions of storage and use, hazardous
Reactivity : 10X RT Buffer No specific test data related to reactivity available for this product or its ingredients.
PCR Nucleotide Mix, 10 mM Solution
No specific test data related to reactivity available for this product or its ingredients.
RNase Inhibitor No specific test data related to reactivity available for this product or its ingredients.
Water, RNase-Free, DEPC Treated
No specific test data related to reactivity available for this product or its ingredients.
MMLV-RT No specific test data related to reactivity available for this product or its ingredients.
50 µM Anchored Oligo dT23VN
No specific test data related to reactivity available for this product or its ingredients.
75 µM Random Hexamer No specific test data related to reactivity available for this product or its ingredients.
10X Primer Mix No specific test data related to reactivity available for this product or its ingredients.
0.1 mg/ml HeLa RNA No specific test data related to reactivity available for this product or its ingredients.
Control Primer Mix for qPCR No specific test data related to reactivity available for this product or its ingredients.
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 10. Stability and reactivity
Hazardous decomposition products
Conditions to avoid 10X RT Buffer No specific data.PCR Nucleotide Mix, 10 mM Solution
No specific data.
RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated
No specific data.
MMLV-RT No specific data.50 µM Anchored Oligo dT23VN
No specific data.
75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.
10X RT Buffer Under normal conditions of storage and use, hazardous decomposition products should not be produced.
PCR Nucleotide Mix, 10 mM Solution
Under normal conditions of storage and use, hazardous decomposition products should not be produced.
RNase Inhibitor Under normal conditions of storage and use, hazardous decomposition products should not be produced.
Water, RNase-Free, DEPC Treated
Under normal conditions of storage and use, hazardous decomposition products should not be produced.
MMLV-RT Under normal conditions of storage and use, hazardous decomposition products should not be produced.
50 µM Anchored Oligo dT23VN
Under normal conditions of storage and use, hazardous decomposition products should not be produced.
75 µM Random Hexamer Under normal conditions of storage and use, hazardous decomposition products should not be produced.
10X Primer Mix Under normal conditions of storage and use, hazardous decomposition products should not be produced.
0.1 mg/ml HeLa RNA Under normal conditions of storage and use, hazardous decomposition products should not be produced.
Control Primer Mix for qPCR Under normal conditions of storage and use, hazardous decomposition products should not be produced.
10X RT Buffer No specific data.PCR Nucleotide Mix, 10 mM Solution
No specific data.
RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated
No specific data.
MMLV-RT No specific data.50 µM Anchored Oligo dT23VN
No specific data.
75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.
:
:
Incompatible materials :
reactions will not occur.0.1 mg/ml HeLa RNA Under normal conditions of storage and use, hazardous
reactions will not occur.Control Primer Mix for qPCR Under normal conditions of storage and use, hazardous
reactions will not occur.
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 11. Toxicological information
Acute toxicity
10X RT Bufferpotassium chloride LD50 Oral Rat 2600 mg/kg -
RNase Inhibitorglycerol LD50 Dermal Rat 21900 mg/kg -
LD50 Oral Rat 12600 mg/kg -
MMLV-RTglycerol LD50 Dermal Rat 21900 mg/kg -
LD50 Oral Rat 12600 mg/kg -
Product/ingredient name Result Species Dose Exposure
Carcinogenicity
Not available.
Mutagenicity
Not available.
Teratogenicity
Not available.
Reproductive toxicity
Not available.
Irritation/Corrosion
10X RT Bufferpotassium chloride Eyes - Mild irritant Rabbit - 24 hours 500
milligrams-
trometamol Skin - Moderate irritant Rabbit - 25 Percent -Skin - Severe irritant Rabbit - 500
milligrams-
Skin - Moderate irritant Woman - 1 Percent -
RNase Inhibitorglycerol Eyes - Mild irritant Rabbit - 24 hours 500
milligrams-
Skin - Mild irritant Rabbit - 24 hours 500 milligrams
-
MMLV-RTglycerol Eyes - Mild irritant Rabbit - 24 hours 500
milligrams-
Skin - Mild irritant Rabbit - 24 hours 500 milligrams
-
Product/ingredient name Result Score Exposure Observation
Sensitization
Not available.
Species
Specific target organ toxicity (single exposure)
Information on toxicological effects
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 11. Toxicological information
Not available.
Information on the likely routes of exposure
Inhalation :
:Ingestion
Skin contact :
:Eye contact
General :
Carcinogenicity :
Mutagenicity :
Teratogenicity :
Developmental effects :
Fertility effects :
Symptoms related to the physical, chemical and toxicological characteristics
Skin contact
Ingestion
Inhalation
:
:
:
Eye contact :
Potential chronic health effects
Delayed and immediate effects and also chronic effects from short and long term exposure
Specific target organ toxicity (repeated exposure)
10X RT Buffer2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride Category 3 Not applicable. Respiratory tract
irritationtrometamol Category 3 Not applicable. Respiratory tract
irritation(R*,R*)-1,4-dimercaptobutane-2,3-diol Category 3 Not applicable. Respiratory tract
irritation
Name Category
Not available.
Numerical measures of toxicity
Aspiration hazard
Not available.
Route of exposure
Target organs
: Not available.
Potential acute health effects
Potential immediate effects
: Not available.
Short term exposure
Potential delayed effects : Not available.
Potential immediate effects
:
Long term exposure
Potential delayed effects :
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 11. Toxicological information
Other information :
10X RT BufferOral 46532.4 mg/kg
Route ATE value
Acute toxicity estimates
Interactive effects :
Section 12. Ecological information
LogPow BCF Potential
Bioaccumulative potential
Product/ingredient name
RNase Inhibitorglycerol -1.76 - low
Water, RNase-Free, DEPC Treatedwater -1.38 - low
MMLV-RTglycerol -1.76 - low
Toxicity
10X RT Bufferpotassium chloride Acute EC50 1337000 µg/l Fresh water Algae - Navicula seminulum 96 hours
Acute EC50 9.24 g/L Fresh water Algae - Desmodesmus subspicatus
72 hours
Acute EC50 83000 µg/l Fresh water Daphnia - Daphnia magna 48 hoursAcute LC50 9.68 mg/l Fresh water Crustaceans - Pseudosida
ramosa - Neonate48 hours
Acute LC50 880000 µg/l Fresh water Fish - Pimephales promelas 96 hours(R*,R*)-1,4-dimercaptobutane-2,3-diol
Acute LC50 27000 µg/l Fresh water Daphnia - Daphnia magna 48 hours
Product/ingredient name SpeciesResult Exposure
Persistence and degradability
Soil/water partition coefficient (KOC)
: 10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution
Not available.
RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated
Not available.
MMLV-RT Not available.50 µM Anchored Oligo dT23VN
Not available.
75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.
Mobility in soil
Not available.
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 12. Ecological information
Mobility :
Other adverse effects : No known significant effects or critical hazards.
Control Primer Mix for qPCR Not available.
Section 13. Disposal considerationsThe generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction.Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Empty containers or liners may retain some product residues. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers.
:Disposal methods
Section 14. Transport information
-
-
-
-
-
-
-
Not regulated.
-
-
Not regulated. Not regulated.
- - -
DOT Classification
IMDG IATA
UN number
UN proper shipping name
Transport hazard class(es)
Packing group
Additional information
Environmental hazards
Special precautions for user
Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code
No. No. No.
Transport within user’s premises: always transport in closed containers that are upright and secure. Ensure that persons transporting the product know what to do in the event of an accident or spillage.
: Not available.
:
TDG Classification
Not regulated.
-
-
-
No.
-
ADR/RID
Not regulated.
-
-
-
No.
-
Mexico Classification
Not regulated.
-
-
-
No.
-
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 15. Regulatory informationU.S. Federal regulations
The following components are listed: GLYCERINE MIST
WARNING: This product contains less than 0.1% of a chemical known to the State of California to cause cancer.
Massachusetts
:
:
California Prop. 65
Clean Air Act Section 112(b) Hazardous Air Pollutants (HAPs)
: Not listed
Clean Air Act Section 602 Class I Substances
: Not listed
Clean Air Act Section 602 Class II Substances
: Not listed
DEA List I Chemicals (Precursor Chemicals)
: Not listed
DEA List II Chemicals (Essential Chemicals)
: Not listed
New York : None of the components are listed.
New Jersey : The following components are listed: GLYCERIN; 1,2,3-PROPANETRIOL
Pennsylvania : The following components are listed: 1,2,3-PROPANETRIOL
State regulations
TSCA 8(a) PAIR: Poly(oxy-1,2-ethanediyl), α-[4-(1,1,3,3-tetramethylbutyl)phenyl]-ω-hydroxy-
TSCA 8(a) CDR Exempt/Partial exemption: Not determined
United States inventory (TSCA 8b): Not determined.
SARA 302/304
SARA 304 RQ : Not applicable.
No products were found.
Composition/information on ingredients
SARA 311/312
Classification : Not applicable.
10X RT Bufferpotassium chloride 1 - 10 No. No. No. Yes. No.2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride
1 - 10 No. No. No. Yes. No.
trometamol 1 - 10 No. No. No. Yes. No.(R*,R*)-1,4-dimercaptobutane-2,3-diol 0 - 1 No. No. No. Yes. No.
RNase Inhibitorglycerol 25 - 50 No. No. No. Yes. No.
MMLV-RTglycerol 25 - 50 No. No. No. Yes. No.
Name % Fire hazard
Sudden release of pressure
Reactive Immediate (acute)health hazard
Delayed (chronic)health hazard
Composition/information on ingredients
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 15. Regulatory information
MMLV-RTPoly(oxy-1,2-ethanediyl), α-[4-(1,1,3,3-tetramethylbutyl)phenyl]-ω-hydroxy-
Yes. No. No. No.
Ingredient name Cancer Reproductive No significant risk level
Maximum acceptable dosage level
International regulations
Chemical Weapon Convention List Schedules I, II & III Chemicals
Montreal Protocol (Annexes A, B, C, E)
Not listed.
Stockholm Convention on Persistent Organic Pollutants
Not listed.
Rotterdam Convention on Prior Inform Consent (PIC)
Not listed.
Not listed.
UNECE Aarhus Protocol on POPs and Heavy Metals
Not listed.
None of the components are listed.
WHMIS (Canada) 10X RT Buffer Class D-2B: Material causing other toxic effects (Toxic).PCR Nucleotide Mix, 10 mM Solution
Not controlled under WHMIS (Canada).
RNase Inhibitor Not controlled under WHMIS (Canada).Water, RNase-Free, DEPC Treated
Not controlled under WHMIS (Canada).
MMLV-RT Not controlled under WHMIS (Canada).50 µM Anchored Oligo dT23VN
Not controlled under WHMIS (Canada).
75 µM Random Hexamer Not controlled under WHMIS (Canada).10X Primer Mix Not controlled under WHMIS (Canada).0.1 mg/ml HeLa RNA Not controlled under WHMIS (Canada).Control Primer Mix for qPCR Not controlled under WHMIS (Canada).
:
Canada
Canadian NPRI :
Canada inventory : Not determined.
CEPA Toxic substances : None of the components are listed.
Canadian lists
Section 16. Other informationHazardous Material Information System (U.S.A.)
0
0
0
National Fire Protection Association (U.S.A.)
Health
Flammability
Physical hazards
The customer is responsible for determining the PPE code for this material.
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First-Strand cDNA Synthesis Kit for Real-Time PCR
Section 16. Other information
History
Date of issue/Date of revision
Version
To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein.Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.
Notice to reader
Date of previous issue
:
:
:
Indicates information that has changed from previously issued version.
Key to abbreviations : ATE = Acute Toxicity EstimateBCF = Bioconcentration FactorGHS = Globally Harmonized System of Classification and Labelling of ChemicalsIATA = International Air Transport AssociationIBC = Intermediate Bulk ContainerIMDG = International Maritime Dangerous GoodsLogPow = logarithm of the octanol/water partition coefficientMARPOL 73/78 = International Convention for the Prevention of Pollution From Ships,1973 as modified by the Protocol of 1978. ("Marpol" = marine pollution)UN = United Nations
11/24/2015.
No previous validation.
1
00
2Health
Special
Instability/Reactivity
Flammability
32/32