SAFETY DATA SHEET · First-Strand cDNA Synthesis Kit for Real-Time PCR Section 2. Hazards...

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First-Strand cDNA Synthesis Kit for Real-Time PCR Not available. SAFETY DATA SHEET GHS product identifier Other means of identification Section 1. Identification : : First-Strand cDNA Synthesis Kit for Real-Time PCR 3420 Central Expressway, Santa Clara CA 95051 Supplier/Manufacturer In case of emergency : : Chemtrec: 1 800 424 9300 Outside USA & Canada: +1 703 527 3887 75780 Code : Section 2. Hazards identification OSHA/HCS status : 10X RT Buffer This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). PCR Nucleotide Mix, 10 mM Solution While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910. 1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. RNase Inhibitor This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). Water, RNase-Free, DEPC Treated While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910. 1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. MMLV-RT This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200). 50 µM Anchored Oligo dT23VN While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910. 1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. 75 µM Random Hexamer While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910. 1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. 10X Primer Mix While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910. 1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. 0.1 mg/ml HeLa RNA While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910. 1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product. Control Primer Mix for qPCR While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910. 1/32

Transcript of SAFETY DATA SHEET · First-Strand cDNA Synthesis Kit for Real-Time PCR Section 2. Hazards...

Page 1: SAFETY DATA SHEET · First-Strand cDNA Synthesis Kit for Real-Time PCR Section 2. Hazards identification 10X RT Buffer SERIOUS EYE DAMAGE/ EYE IRRITATION - Category 2B SKIN SENSITIZATION

First-Strand cDNA Synthesis Kit for Real-Time PCR

Not available.

SAFETY DATA SHEET

GHS product identifier

Other means of identification

Section 1. Identification:

:

First-Strand cDNA Synthesis Kit for Real-Time PCR

3420 Central Expressway, Santa Clara CA 95051Supplier/Manufacturer

In case of emergency :

:

Chemtrec: 1 800 424 9300Outside USA & Canada: +1 703 527 3887

75780Code :

Section 2. Hazards identificationOSHA/HCS status : 10X RT Buffer This material is considered hazardous by the OSHA

Hazard Communication Standard (29 CFR 1910.1200).PCR Nucleotide Mix, 10 mM Solution

While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.

RNase Inhibitor This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200).

Water, RNase-Free, DEPC Treated

While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.

MMLV-RT This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200).

50 µM Anchored Oligo dT23VN

While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.

75 µM Random Hexamer While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.

10X Primer Mix While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.

0.1 mg/ml HeLa RNA While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.

Control Primer Mix for qPCR While this material is not considered hazardous by the OSHA Hazard Communication Standard (29 CFR 1910.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 2. Hazards identification

10X RT Buffer SERIOUS EYE DAMAGE/ EYE IRRITATION - Category 2BSKIN SENSITIZATION - Category 1

PCR Nucleotide Mix, 10 mM Solution

Not classified.

RNase Inhibitor SERIOUS EYE DAMAGE/ EYE IRRITATION - Category 2B

Water, RNase-Free, DEPC Treated

Not classified.

MMLV-RT SERIOUS EYE DAMAGE/ EYE IRRITATION - Category 2B

50 µM Anchored Oligo dT23VN

Not classified.

75 µM Random Hexamer Not classified.10X Primer Mix Not classified.0.1 mg/ml HeLa RNA Not classified.Control Primer Mix for qPCR Not classified.

Classification of the substance or mixture

:

Signal word : 10X RT Buffer WarningPCR Nucleotide Mix, 10 mM Solution

No signal word.

RNase Inhibitor WarningWater, RNase-Free, DEPC Treated

No signal word.

MMLV-RT Warning50 µM Anchored Oligo dT23VN

No signal word.

75 µM Random Hexamer No signal word.10X Primer Mix No signal word.0.1 mg/ml HeLa RNA No signal word.Control Primer Mix for qPCR No signal word.

Hazard statements : 10X RT Buffer Causes eye irritation.May cause an allergic skin reaction.

PCR Nucleotide Mix, 10 mM Solution

No known significant effects or critical hazards.

RNase Inhibitor Causes eye irritation.Water, RNase-Free, DEPC Treated

No known significant effects or critical hazards.

MMLV-RT Causes eye irritation.50 µM Anchored Oligo dT23VN

No known significant effects or critical hazards.

75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.

Hazard pictograms :

Precautionary statements

GHS label elements

1200), this MSDS contains valuable information critical to the safe handling and proper use of the product. This MSDS should be retained and available for employees and other users of this product.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 2. Hazards identification

Prevention : 10X RT Buffer Wear protective gloves. Wear eye or face protection.Avoid breathing vapor. Wash hands thoroughly after handling. Contaminated work clothing should not be allowed out of the workplace.

PCR Nucleotide Mix, 10 mM Solution

Not applicable.

RNase Inhibitor Wear eye or face protection. Wash hands thoroughly after handling.

Water, RNase-Free, DEPC Treated

Not applicable.

MMLV-RT Wear eye or face protection. Wash hands thoroughly after handling.

50 µM Anchored Oligo dT23VN

Not applicable.

75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.

Response : 10X RT Buffer IF ON SKIN: Wash with plenty of soap and water.Wash contaminated clothing before reuse. If skin irritation or rash occurs: Get medical attention. IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do.Continue rinsing. If eye irritation persists: Get medical attention.

PCR Nucleotide Mix, 10 mM Solution

Not applicable.

RNase Inhibitor IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical attention.

Water, RNase-Free, DEPC Treated

Not applicable.

MMLV-RT IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical attention.

50 µM Anchored Oligo dT23VN

Not applicable.

75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.

General : 10X RT Buffer Not applicable.PCR Nucleotide Mix, 10 mM Solution

Not applicable.

RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated

Not applicable.

MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN

Not applicable.

75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 2. Hazards identificationStorage : 10X RT Buffer Not applicable.

PCR Nucleotide Mix, 10 mM Solution

Not applicable.

RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated

Not applicable.

MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN

Not applicable.

75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.

Disposal : 10X RT Buffer Dispose of contents and container in accordance with all local, regional, national and international regulations.

PCR Nucleotide Mix, 10 mM Solution

Not applicable.

RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated

Not applicable.

MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN

Not applicable.

75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.

Supplemental label elements

: 10X RT Buffer None known.PCR Nucleotide Mix, 10 mM Solution

None known.

RNase Inhibitor None known.Water, RNase-Free, DEPC Treated

None known.

MMLV-RT None known.50 µM Anchored Oligo dT23VN

None known.

75 µM Random Hexamer None known.10X Primer Mix None known.0.1 mg/ml HeLa RNA None known.Control Primer Mix for qPCR None known.

Hazards not otherwise classified

: 10X RT Buffer None known.PCR Nucleotide Mix, 10 mM Solution

None known.

RNase Inhibitor None known.Water, RNase-Free, DEPC Treated

None known.

MMLV-RT None known.50 µM Anchored Oligo dT23VN

None known.

75 µM Random Hexamer None known.10X Primer Mix None known.0.1 mg/ml HeLa RNA None known.Control Primer Mix for qPCR None known.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 3. Composition/information on ingredients

10X RT Bufferpotassium chloride 1 - 10 7447-40-72-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride 1 - 10 1185-53-1trometamol 1 - 10 77-86-1(R*,R*)-1,4-dimercaptobutane-2,3-diol 0 - 1 3483-12-3

RNase Inhibitorglycerol 25 - 50 56-81-5

MMLV-RTglycerol 25 - 50 56-81-5

Ingredient name CAS number%

There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section.

Substance/mixture :

Occupational exposure limits, if available, are listed in Section 8.

Mixture

Any concentration shown as a range is to protect confidentiality or is due to batch variation.

10X RT Buffer Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Continue to rinse for at least 10 minutes. If irritation persists, get medical attention.

PCR Nucleotide Mix, 10 mM Solution

Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.

RNase Inhibitor Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Continue to rinse for at least 10 minutes. If irritation persists, get medical attention.

Water, RNase-Free, DEPC Treated

Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.

MMLV-RT Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Continue to rinse for at least 10 minutes. If irritation persists, get medical attention.

50 µM Anchored Oligo dT23VN

Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.

75 µM Random Hexamer Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.

10X Primer Mix Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check

Section 4. First aid measures

Eye contact :

Description of necessary first aid measures

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 4. First aid measuresfor and remove any contact lenses. Get medical attention if irritation occurs.

0.1 mg/ml HeLa RNA Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.

Control Primer Mix for qPCR Immediately flush eyes with plenty of water,occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs.

10X RT Buffer Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention if adverse health effects persist or are severe. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband. In case of inhalation of decomposition products in a fire, symptoms may be delayed. The exposed person may need to be kept under medical surveillance for 48 hours.

PCR Nucleotide Mix, 10 mM Solution

Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.

RNase Inhibitor Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention if adverse health effects persist or are severe. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.

Water, RNase-Free, DEPC Treated

Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.

MMLV-RT Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Get medical attention if adverse health effects persist or are severe. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.

50 µM Anchored Oligo dT23VN

Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.

75 µM Random Hexamer Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.

10X Primer Mix Remove victim to fresh air and keep at rest in a position

Inhalation :

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 4. First aid measures

10X RT Buffer Wash out mouth with water. Remove dentures if any.Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention if adverse health effects persist or are severe.Never give anything by mouth to an unconscious person.If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.

10X RT Buffer Wash with plenty of soap and water. Remove contaminated clothing and shoes. Wash contaminated clothing thoroughly with water before removing it, or wear gloves. Continue to rinse for at least 10 minutes.Get medical attention. In the event of any complaints or symptoms, avoid further exposure. Wash clothing before reuse. Clean shoes thoroughly before reuse.

PCR Nucleotide Mix, 10 mM Solution

Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.

RNase Inhibitor Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur. Wash clothing before reuse. Clean shoes thoroughly before reuse.

Water, RNase-Free, DEPC Treated

Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.

MMLV-RT Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur. Wash clothing before reuse. Clean shoes thoroughly before reuse.

50 µM Anchored Oligo dT23VN

Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.

75 µM Random Hexamer Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.

10X Primer Mix Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.

0.1 mg/ml HeLa RNA Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.

Control Primer Mix for qPCR Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur.

comfortable for breathing. Get medical attention if symptoms occur.

0.1 mg/ml HeLa RNA Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.

Control Primer Mix for qPCR Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur.

Skin contact

Ingestion :

:

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 4. First aid measuresPCR Nucleotide Mix, 10 mM Solution

Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.

RNase Inhibitor Wash out mouth with water. Remove dentures if any.Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention if adverse health effects persist or are severe.Never give anything by mouth to an unconscious person.If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.

Water, RNase-Free, DEPC Treated

Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.

MMLV-RT Wash out mouth with water. Remove dentures if any.Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention if adverse health effects persist or are severe.Never give anything by mouth to an unconscious person.If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway.Loosen tight clothing such as a collar, tie, belt or waistband.

50 µM Anchored Oligo dT23VN

Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.

75 µM Random Hexamer Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.

10X Primer Mix Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 4. First aid measurespersonnel. Get medical attention if symptoms occur.

0.1 mg/ml HeLa RNA Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.

Control Primer Mix for qPCR Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing.If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur.

Most important symptoms/effects, acute and delayed

Inhalation : 10X RT Buffer Exposure to decomposition products may cause a health hazard. Serious effects may be delayed following exposure.

PCR Nucleotide Mix, 10 mM Solution

No known significant effects or critical hazards.

RNase Inhibitor No known significant effects or critical hazards.Water, RNase-Free, DEPC Treated

No known significant effects or critical hazards.

MMLV-RT No known significant effects or critical hazards.50 µM Anchored Oligo dT23VN

No known significant effects or critical hazards.

75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.

Skin contact : 10X RT Buffer May cause an allergic skin reaction.PCR Nucleotide Mix, 10 mM Solution

No known significant effects or critical hazards.

RNase Inhibitor No known significant effects or critical hazards.Water, RNase-Free, DEPC Treated

No known significant effects or critical hazards.

MMLV-RT No known significant effects or critical hazards.50 µM Anchored Oligo dT23VN

No known significant effects or critical hazards.

75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.

10X RT Buffer Causes eye irritation.PCR Nucleotide Mix, 10 mM Solution

No known significant effects or critical hazards.

RNase Inhibitor Causes eye irritation.Water, RNase-Free, DEPC Treated

No known significant effects or critical hazards.

MMLV-RT Causes eye irritation.50 µM Anchored Oligo dT23VN

No known significant effects or critical hazards.

75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.

:Eye contact

Potential acute health effects

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 4. First aid measures10X RT Buffer May be irritating to mouth, throat and stomach.PCR Nucleotide Mix, 10 mM Solution

No known significant effects or critical hazards.

RNase Inhibitor May be irritating to mouth, throat and stomach.Water, RNase-Free, DEPC Treated

No known significant effects or critical hazards.

MMLV-RT May be irritating to mouth, throat and stomach.50 µM Anchored Oligo dT23VN

No known significant effects or critical hazards.

75 µM Random Hexamer No known significant effects or critical hazards.10X Primer Mix No known significant effects or critical hazards.0.1 mg/ml HeLa RNA No known significant effects or critical hazards.Control Primer Mix for qPCR No known significant effects or critical hazards.

:Ingestion

Over-exposure signs/symptoms

Skin contact

Inhalation 10X RT Buffer No specific data.PCR Nucleotide Mix, 10 mM Solution

No specific data.

RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated

No specific data.

MMLV-RT No specific data.50 µM Anchored Oligo dT23VN

No specific data.

75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.

10X RT Buffer Adverse symptoms may include the following:irritationredness

PCR Nucleotide Mix, 10 mM Solution

No specific data.

RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated

No specific data.

MMLV-RT No specific data.

:

:

Eye contact : 10X RT Buffer Adverse symptoms may include the following:irritationwateringredness

PCR Nucleotide Mix, 10 mM Solution

No specific data.

RNase Inhibitor Adverse symptoms may include the following:irritationwateringredness

Water, RNase-Free, DEPC Treated

No specific data.

MMLV-RT Adverse symptoms may include the following:irritationwateringredness

50 µM Anchored Oligo dT23VN

No specific data.

75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 4. First aid measures

Protection of first-aiders : No action shall be taken involving any personal risk or without suitable training.

Notes to physician : Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled.

Specific treatments : No specific treatment.

Ingestion 10X RT Buffer No specific data.PCR Nucleotide Mix, 10 mM Solution

No specific data.

RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated

No specific data.

MMLV-RT No specific data.50 µM Anchored Oligo dT23VN

No specific data.

75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.

50 µM Anchored Oligo dT23VN

No specific data.

75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.

:

See toxicological information (Section 11)

Indication of immediate medical attention and special treatment needed, if necessary

Section 5. Fire-fighting measures

10X RT Buffer Use an extinguishing agent suitable for the surrounding fire.

PCR Nucleotide Mix, 10 mM Solution

Use an extinguishing agent suitable for the surrounding fire.

RNase Inhibitor Use an extinguishing agent suitable for the surrounding fire.

Water, RNase-Free, DEPC Treated

Use an extinguishing agent suitable for the surrounding fire.

MMLV-RT Use an extinguishing agent suitable for the surrounding fire.

50 µM Anchored Oligo dT23VN

Use an extinguishing agent suitable for the surrounding fire.

75 µM Random Hexamer Use an extinguishing agent suitable for the surrounding fire.

10X Primer Mix Use an extinguishing agent suitable for the surrounding fire.

0.1 mg/ml HeLa RNA Use an extinguishing agent suitable for the surrounding fire.

Control Primer Mix for qPCR Use an extinguishing agent suitable for the surrounding fire.

Extinguishing media

Suitable extinguishing media

:

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 5. Fire-fighting measures

Hazardous thermal decomposition products

Specific hazards arising from the chemical

10X RT Buffer Decomposition products may include the following materials:carbon dioxidecarbon monoxidenitrogen oxideshalogenated compoundsmetal oxide/oxides

PCR Nucleotide Mix, 10 mM Solution

No specific data.

RNase Inhibitor Decomposition products may include the following materials:carbon dioxidecarbon monoxide

Water, RNase-Free, DEPC Treated

No specific data.

MMLV-RT Decomposition products may include the following materials:carbon dioxidecarbon monoxide

50 µM Anchored Oligo dT23VN

No specific data.

75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.

10X RT Buffer In a fire or if heated, a pressure increase will occur and the container may burst.

PCR Nucleotide Mix, 10 mM Solution

In a fire or if heated, a pressure increase will occur and the container may burst.

RNase Inhibitor In a fire or if heated, a pressure increase will occur and the container may burst.

Water, RNase-Free, DEPC Treated

In a fire or if heated, a pressure increase will occur and the container may burst.

MMLV-RT In a fire or if heated, a pressure increase will occur and the container may burst.

50 µM Anchored Oligo dT23VN

In a fire or if heated, a pressure increase will occur and the container may burst.

75 µM Random Hexamer In a fire or if heated, a pressure increase will occur and the container may burst.

10X Primer Mix In a fire or if heated, a pressure increase will occur and the container may burst.

0.1 mg/ml HeLa RNA In a fire or if heated, a pressure increase will occur and the container may burst.

Control Primer Mix for qPCR In a fire or if heated, a pressure increase will occur and the container may burst.

:

:

10X RT Buffer None known.PCR Nucleotide Mix, 10 mM Solution

None known.

RNase Inhibitor None known.Water, RNase-Free, DEPC Treated

None known.

MMLV-RT None known.50 µM Anchored Oligo dT23VN

None known.

75 µM Random Hexamer None known.10X Primer Mix None known.0.1 mg/ml HeLa RNA None known.Control Primer Mix for qPCR None known.

Unsuitable extinguishing media

:

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 5. Fire-fighting measures

10X RT Buffer Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

PCR Nucleotide Mix, 10 mM Solution

Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

RNase Inhibitor Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

Water, RNase-Free, DEPC Treated

Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

MMLV-RT Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

50 µM Anchored Oligo dT23VN

Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

75 µM Random Hexamer Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

10X Primer Mix Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

0.1 mg/ml HeLa RNA Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

Control Primer Mix for qPCR Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training.

Control Primer Mix for qPCR No specific data.

10X RT Buffer Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

PCR Nucleotide Mix, 10 mM Solution

Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

RNase Inhibitor Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

Water, RNase-Free, DEPC Treated

Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

MMLV-RT Fire-fighters should wear appropriate protective

Special protective equipment for fire-fighters

:

Special protective actions for fire-fighters

:

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 5. Fire-fighting measuresequipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

50 µM Anchored Oligo dT23VN

Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

75 µM Random Hexamer Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

10X Primer Mix Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

0.1 mg/ml HeLa RNA Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

Control Primer Mix for qPCR Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode.

Section 6. Accidental release measures

Environmental precautions

Personal precautions, protective equipment and emergency procedures

Stop leak if without risk. Move containers from spill area. Prevent entry into sewers,water courses, basements or confined areas. Wash spillages into an effluent treatment plant or proceed as follows. Contain and collect spillage with non-combustible,absorbent material e.g. sand, earth, vermiculite or diatomaceous earth and place in container for disposal according to local regulations (see Section 13). Dispose of via a licensed waste disposal contractor. Note: see Section 1 for emergency contact information and Section 13 for waste disposal.

:

: No action shall be taken involving any personal risk or without suitable training.Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Put on appropriate personal protective equipment.

Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air).

Large spill :

Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor.

Small spill :

Methods and materials for containment and cleaning up

For non-emergency personnel

For emergency responders : If specialised clothing is required to deal with the spillage, take note of any information in Section 8 on suitable and unsuitable materials. See also the information in "For non-emergency personnel".

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 7. Handling and storage

Advice on general occupational hygiene

10X RT Buffer Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

PCR Nucleotide Mix, 10 mM Solution

Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

RNase Inhibitor Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before

:

Protective measures 10X RT Buffer Put on appropriate personal protective equipment (see Section 8). Persons with a history of skin sensitization problems should not be employed in any process in which this product is used. Do not get in eyes or on skin or clothing. Do not ingest. Avoid breathing vapor or mist. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container.

PCR Nucleotide Mix, 10 mM Solution

Put on appropriate personal protective equipment (see Section 8).

RNase Inhibitor Put on appropriate personal protective equipment (see Section 8). Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container.

Water, RNase-Free, DEPC Treated

Put on appropriate personal protective equipment (see Section 8).

MMLV-RT Put on appropriate personal protective equipment (see Section 8). Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container.

50 µM Anchored Oligo dT23VN

Put on appropriate personal protective equipment (see Section 8).

75 µM Random Hexamer Put on appropriate personal protective equipment (see Section 8).

10X Primer Mix Put on appropriate personal protective equipment (see Section 8).

0.1 mg/ml HeLa RNA Put on appropriate personal protective equipment (see Section 8).

Control Primer Mix for qPCR Put on appropriate personal protective equipment (see Section 8).

:

Precautions for safe handling

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 7. Handling and storageentering eating areas. See also Section 8 for additional information on hygiene measures.

Water, RNase-Free, DEPC Treated

Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

MMLV-RT Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

50 µM Anchored Oligo dT23VN

Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

75 µM Random Hexamer Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

10X Primer Mix Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

0.1 mg/ml HeLa RNA Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

Control Primer Mix for qPCR Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 7. Handling and storageConditions for safe storage,including any incompatibilities

10X RT Buffer Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

PCR Nucleotide Mix, 10 mM Solution

Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

RNase Inhibitor Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

Water, RNase-Free, DEPC Treated

Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

MMLV-RT Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

50 µM Anchored Oligo dT23VN

Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

75 µM Random Hexamer Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.

:

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 7. Handling and storageContainers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

10X Primer Mix Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

0.1 mg/ml HeLa RNA Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

Control Primer Mix for qPCR Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry,cool and well-ventilated area, away from incompatible materials (see Section 10) and food and drink. Keep container tightly closed and sealed until ready for use.Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination.

RNase Inhibitorglycerol OSHA PEL 1989 (United States, 3/1989).

TWA: 5 mg/m³ 8 hours. Form: Respirable fraction TWA: 10 mg/m³ 8 hours. Form: Total dustOSHA PEL (United States, 2/2013). TWA: 5 mg/m³ 8 hours. Form: Respirable fraction TWA: 15 mg/m³ 8 hours. Form: Total dust

MMLV-RTglycerol OSHA PEL 1989 (United States, 3/1989).

TWA: 5 mg/m³ 8 hours. Form: Respirable fraction TWA: 10 mg/m³ 8 hours. Form: Total dustOSHA PEL (United States, 2/2013). TWA: 5 mg/m³ 8 hours. Form: Respirable fraction TWA: 15 mg/m³ 8 hours. Form: Total dust

Section 8. Exposure controls/personal protection

Ingredient name Exposure limits

Control parameters

Occupational exposure limits

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 8. Exposure controls/personal protection

Hand protection

Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator.

Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary.

Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists,gases or dusts. If contact is possible, the following protection should be worn, unless the assessment indicates a higher degree of protection: safety glasses with side-shields.

Eye/face protection

Respiratory protection :

:

:

Body protection Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product.

:

Environmental exposure controls

: Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels.

Appropriate engineering controls

: Good general ventilation should be sufficient to control worker exposure to airborne contaminants.

Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period.Appropriate techniques should be used to remove potentially contaminated clothing.Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location.

Hygiene measures :

Individual protection measures

Skin protection

Other skin protection : Appropriate footwear and any additional skin protection measures should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product.

Section 9. Physical and chemical properties10X RT Buffer Liquid.PCR Nucleotide Mix, 10 mM Solution

Liquid.

RNase Inhibitor Liquid.Water, RNase-Free, DEPC Treated

Liquid.

MMLV-RT Liquid.50 µM Anchored Oligo dT23VN

Liquid.

75 µM Random Hexamer Liquid.10X Primer Mix Liquid.0.1 mg/ml HeLa RNA Liquid.Control Primer Mix for qPCR Liquid.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

Physical state

Color

:

:

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 9. Physical and chemical properties

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

Odor :

Flash point : 10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

:Auto-ignition temperature

Flammable limits : 10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 9. Physical and chemical properties

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

100°C (212°F)

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not applicable.PCR Nucleotide Mix, 10 mM Solution

Not applicable.

RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated

18.02 g/mole

MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN

Not applicable.

75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.

Boiling/condensation point

Molecular weight

pH

10X RT Buffer Not applicable.PCR Nucleotide Mix, 10 mM Solution

Not applicable.

RNase Inhibitor Not applicable.Water, RNase-Free, DEPC Treated

H2-O

MMLV-RT Not applicable.50 µM Anchored Oligo dT23VN

Not applicable.

75 µM Random Hexamer Not applicable.10X Primer Mix Not applicable.0.1 mg/ml HeLa RNA Not applicable.Control Primer Mix for qPCR Not applicable.

Molecular formula

:

:

:

:

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 9. Physical and chemical properties10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

0°C (32°F)

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

3.2 kPa (23.8 mm Hg) [room temperature]

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

Melting/freezing point

Relative density

Vapor pressure

Vapor density

:

:

:

:

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Section 9. Physical and chemical properties10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.water Not available.MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.

Volatility

Evaporation rate

Viscosity

Solubility

Physical/chemical properties comments

:

:

:

:

:

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 9. Physical and chemical properties50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.Control Primer Mix for qPCR Not available.

Section 10. Stability and reactivity

10X RT Buffer The product is stable.PCR Nucleotide Mix, 10 mM Solution

The product is stable.

RNase Inhibitor The product is stable.Water, RNase-Free, DEPC Treated

The product is stable.

MMLV-RT The product is stable.50 µM Anchored Oligo dT23VN

The product is stable.

75 µM Random Hexamer The product is stable.10X Primer Mix The product is stable.0.1 mg/ml HeLa RNA The product is stable.Control Primer Mix for qPCR The product is stable.

Chemical stability :

Possibility of hazardous reactions

: 10X RT Buffer Under normal conditions of storage and use, hazardous reactions will not occur.

PCR Nucleotide Mix, 10 mM Solution

Under normal conditions of storage and use, hazardous reactions will not occur.

RNase Inhibitor Under normal conditions of storage and use, hazardous reactions will not occur.

Water, RNase-Free, DEPC Treated

Under normal conditions of storage and use, hazardous reactions will not occur.

MMLV-RT Under normal conditions of storage and use, hazardous reactions will not occur.

50 µM Anchored Oligo dT23VN

Under normal conditions of storage and use, hazardous reactions will not occur.

75 µM Random Hexamer Under normal conditions of storage and use, hazardous reactions will not occur.

10X Primer Mix Under normal conditions of storage and use, hazardous

Reactivity : 10X RT Buffer No specific test data related to reactivity available for this product or its ingredients.

PCR Nucleotide Mix, 10 mM Solution

No specific test data related to reactivity available for this product or its ingredients.

RNase Inhibitor No specific test data related to reactivity available for this product or its ingredients.

Water, RNase-Free, DEPC Treated

No specific test data related to reactivity available for this product or its ingredients.

MMLV-RT No specific test data related to reactivity available for this product or its ingredients.

50 µM Anchored Oligo dT23VN

No specific test data related to reactivity available for this product or its ingredients.

75 µM Random Hexamer No specific test data related to reactivity available for this product or its ingredients.

10X Primer Mix No specific test data related to reactivity available for this product or its ingredients.

0.1 mg/ml HeLa RNA No specific test data related to reactivity available for this product or its ingredients.

Control Primer Mix for qPCR No specific test data related to reactivity available for this product or its ingredients.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 10. Stability and reactivity

Hazardous decomposition products

Conditions to avoid 10X RT Buffer No specific data.PCR Nucleotide Mix, 10 mM Solution

No specific data.

RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated

No specific data.

MMLV-RT No specific data.50 µM Anchored Oligo dT23VN

No specific data.

75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.

10X RT Buffer Under normal conditions of storage and use, hazardous decomposition products should not be produced.

PCR Nucleotide Mix, 10 mM Solution

Under normal conditions of storage and use, hazardous decomposition products should not be produced.

RNase Inhibitor Under normal conditions of storage and use, hazardous decomposition products should not be produced.

Water, RNase-Free, DEPC Treated

Under normal conditions of storage and use, hazardous decomposition products should not be produced.

MMLV-RT Under normal conditions of storage and use, hazardous decomposition products should not be produced.

50 µM Anchored Oligo dT23VN

Under normal conditions of storage and use, hazardous decomposition products should not be produced.

75 µM Random Hexamer Under normal conditions of storage and use, hazardous decomposition products should not be produced.

10X Primer Mix Under normal conditions of storage and use, hazardous decomposition products should not be produced.

0.1 mg/ml HeLa RNA Under normal conditions of storage and use, hazardous decomposition products should not be produced.

Control Primer Mix for qPCR Under normal conditions of storage and use, hazardous decomposition products should not be produced.

10X RT Buffer No specific data.PCR Nucleotide Mix, 10 mM Solution

No specific data.

RNase Inhibitor No specific data.Water, RNase-Free, DEPC Treated

No specific data.

MMLV-RT No specific data.50 µM Anchored Oligo dT23VN

No specific data.

75 µM Random Hexamer No specific data.10X Primer Mix No specific data.0.1 mg/ml HeLa RNA No specific data.Control Primer Mix for qPCR No specific data.

:

:

Incompatible materials :

reactions will not occur.0.1 mg/ml HeLa RNA Under normal conditions of storage and use, hazardous

reactions will not occur.Control Primer Mix for qPCR Under normal conditions of storage and use, hazardous

reactions will not occur.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 11. Toxicological information

Acute toxicity

10X RT Bufferpotassium chloride LD50 Oral Rat 2600 mg/kg -

RNase Inhibitorglycerol LD50 Dermal Rat 21900 mg/kg -

LD50 Oral Rat 12600 mg/kg -

MMLV-RTglycerol LD50 Dermal Rat 21900 mg/kg -

LD50 Oral Rat 12600 mg/kg -

Product/ingredient name Result Species Dose Exposure

Carcinogenicity

Not available.

Mutagenicity

Not available.

Teratogenicity

Not available.

Reproductive toxicity

Not available.

Irritation/Corrosion

10X RT Bufferpotassium chloride Eyes - Mild irritant Rabbit - 24 hours 500

milligrams-

trometamol Skin - Moderate irritant Rabbit - 25 Percent -Skin - Severe irritant Rabbit - 500

milligrams-

Skin - Moderate irritant Woman - 1 Percent -

RNase Inhibitorglycerol Eyes - Mild irritant Rabbit - 24 hours 500

milligrams-

Skin - Mild irritant Rabbit - 24 hours 500 milligrams

-

MMLV-RTglycerol Eyes - Mild irritant Rabbit - 24 hours 500

milligrams-

Skin - Mild irritant Rabbit - 24 hours 500 milligrams

-

Product/ingredient name Result Score Exposure Observation

Sensitization

Not available.

Species

Specific target organ toxicity (single exposure)

Information on toxicological effects

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 11. Toxicological information

Not available.

Information on the likely routes of exposure

Inhalation :

:Ingestion

Skin contact :

:Eye contact

General :

Carcinogenicity :

Mutagenicity :

Teratogenicity :

Developmental effects :

Fertility effects :

Symptoms related to the physical, chemical and toxicological characteristics

Skin contact

Ingestion

Inhalation

:

:

:

Eye contact :

Potential chronic health effects

Delayed and immediate effects and also chronic effects from short and long term exposure

Specific target organ toxicity (repeated exposure)

10X RT Buffer2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride Category 3 Not applicable. Respiratory tract

irritationtrometamol Category 3 Not applicable. Respiratory tract

irritation(R*,R*)-1,4-dimercaptobutane-2,3-diol Category 3 Not applicable. Respiratory tract

irritation

Name Category

Not available.

Numerical measures of toxicity

Aspiration hazard

Not available.

Route of exposure

Target organs

: Not available.

Potential acute health effects

Potential immediate effects

: Not available.

Short term exposure

Potential delayed effects : Not available.

Potential immediate effects

:

Long term exposure

Potential delayed effects :

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 11. Toxicological information

Other information :

10X RT BufferOral 46532.4 mg/kg

Route ATE value

Acute toxicity estimates

Interactive effects :

Section 12. Ecological information

LogPow BCF Potential

Bioaccumulative potential

Product/ingredient name

RNase Inhibitorglycerol -1.76 - low

Water, RNase-Free, DEPC Treatedwater -1.38 - low

MMLV-RTglycerol -1.76 - low

Toxicity

10X RT Bufferpotassium chloride Acute EC50 1337000 µg/l Fresh water Algae - Navicula seminulum 96 hours

Acute EC50 9.24 g/L Fresh water Algae - Desmodesmus subspicatus

72 hours

Acute EC50 83000 µg/l Fresh water Daphnia - Daphnia magna 48 hoursAcute LC50 9.68 mg/l Fresh water Crustaceans - Pseudosida

ramosa - Neonate48 hours

Acute LC50 880000 µg/l Fresh water Fish - Pimephales promelas 96 hours(R*,R*)-1,4-dimercaptobutane-2,3-diol

Acute LC50 27000 µg/l Fresh water Daphnia - Daphnia magna 48 hours

Product/ingredient name SpeciesResult Exposure

Persistence and degradability

Soil/water partition coefficient (KOC)

: 10X RT Buffer Not available.PCR Nucleotide Mix, 10 mM Solution

Not available.

RNase Inhibitor Not available.Water, RNase-Free, DEPC Treated

Not available.

MMLV-RT Not available.50 µM Anchored Oligo dT23VN

Not available.

75 µM Random Hexamer Not available.10X Primer Mix Not available.0.1 mg/ml HeLa RNA Not available.

Mobility in soil

Not available.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 12. Ecological information

Mobility :

Other adverse effects : No known significant effects or critical hazards.

Control Primer Mix for qPCR Not available.

Section 13. Disposal considerationsThe generation of waste should be avoided or minimized wherever possible. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of all authorities with jurisdiction.Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Empty containers or liners may retain some product residues. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers.

:Disposal methods

Section 14. Transport information

-

-

-

-

-

-

-

Not regulated.

-

-

Not regulated. Not regulated.

- - -

DOT Classification

IMDG IATA

UN number

UN proper shipping name

Transport hazard class(es)

Packing group

Additional information

Environmental hazards

Special precautions for user

Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code

No. No. No.

Transport within user’s premises: always transport in closed containers that are upright and secure. Ensure that persons transporting the product know what to do in the event of an accident or spillage.

: Not available.

:

TDG Classification

Not regulated.

-

-

-

No.

-

ADR/RID

Not regulated.

-

-

-

No.

-

Mexico Classification

Not regulated.

-

-

-

No.

-

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 15. Regulatory informationU.S. Federal regulations

The following components are listed: GLYCERINE MIST

WARNING: This product contains less than 0.1% of a chemical known to the State of California to cause cancer.

Massachusetts

:

:

California Prop. 65

Clean Air Act Section 112(b) Hazardous Air Pollutants (HAPs)

: Not listed

Clean Air Act Section 602 Class I Substances

: Not listed

Clean Air Act Section 602 Class II Substances

: Not listed

DEA List I Chemicals (Precursor Chemicals)

: Not listed

DEA List II Chemicals (Essential Chemicals)

: Not listed

New York : None of the components are listed.

New Jersey : The following components are listed: GLYCERIN; 1,2,3-PROPANETRIOL

Pennsylvania : The following components are listed: 1,2,3-PROPANETRIOL

State regulations

TSCA 8(a) PAIR: Poly(oxy-1,2-ethanediyl), α-[4-(1,1,3,3-tetramethylbutyl)phenyl]-ω-hydroxy-

TSCA 8(a) CDR Exempt/Partial exemption: Not determined

United States inventory (TSCA 8b): Not determined.

SARA 302/304

SARA 304 RQ : Not applicable.

No products were found.

Composition/information on ingredients

SARA 311/312

Classification : Not applicable.

10X RT Bufferpotassium chloride 1 - 10 No. No. No. Yes. No.2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride

1 - 10 No. No. No. Yes. No.

trometamol 1 - 10 No. No. No. Yes. No.(R*,R*)-1,4-dimercaptobutane-2,3-diol 0 - 1 No. No. No. Yes. No.

RNase Inhibitorglycerol 25 - 50 No. No. No. Yes. No.

MMLV-RTglycerol 25 - 50 No. No. No. Yes. No.

Name % Fire hazard

Sudden release of pressure

Reactive Immediate (acute)health hazard

Delayed (chronic)health hazard

Composition/information on ingredients

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 15. Regulatory information

MMLV-RTPoly(oxy-1,2-ethanediyl), α-[4-(1,1,3,3-tetramethylbutyl)phenyl]-ω-hydroxy-

Yes. No. No. No.

Ingredient name Cancer Reproductive No significant risk level

Maximum acceptable dosage level

International regulations

Chemical Weapon Convention List Schedules I, II & III Chemicals

Montreal Protocol (Annexes A, B, C, E)

Not listed.

Stockholm Convention on Persistent Organic Pollutants

Not listed.

Rotterdam Convention on Prior Inform Consent (PIC)

Not listed.

Not listed.

UNECE Aarhus Protocol on POPs and Heavy Metals

Not listed.

None of the components are listed.

WHMIS (Canada) 10X RT Buffer Class D-2B: Material causing other toxic effects (Toxic).PCR Nucleotide Mix, 10 mM Solution

Not controlled under WHMIS (Canada).

RNase Inhibitor Not controlled under WHMIS (Canada).Water, RNase-Free, DEPC Treated

Not controlled under WHMIS (Canada).

MMLV-RT Not controlled under WHMIS (Canada).50 µM Anchored Oligo dT23VN

Not controlled under WHMIS (Canada).

75 µM Random Hexamer Not controlled under WHMIS (Canada).10X Primer Mix Not controlled under WHMIS (Canada).0.1 mg/ml HeLa RNA Not controlled under WHMIS (Canada).Control Primer Mix for qPCR Not controlled under WHMIS (Canada).

:

Canada

Canadian NPRI :

Canada inventory : Not determined.

CEPA Toxic substances : None of the components are listed.

Canadian lists

Section 16. Other informationHazardous Material Information System (U.S.A.)

0

0

0

National Fire Protection Association (U.S.A.)

Health

Flammability

Physical hazards

The customer is responsible for determining the PPE code for this material.

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First-Strand cDNA Synthesis Kit for Real-Time PCR

Section 16. Other information

History

Date of issue/Date of revision

Version

To the best of our knowledge, the information contained herein is accurate. However, neither the above-named supplier, nor any of its subsidiaries, assumes any liability whatsoever for the accuracy or completeness of the information contained herein.Final determination of suitability of any material is the sole responsibility of the user. All materials may present unknown hazards and should be used with caution. Although certain hazards are described herein, we cannot guarantee that these are the only hazards that exist.

Notice to reader

Date of previous issue

:

:

:

Indicates information that has changed from previously issued version.

Key to abbreviations : ATE = Acute Toxicity EstimateBCF = Bioconcentration FactorGHS = Globally Harmonized System of Classification and Labelling of ChemicalsIATA = International Air Transport AssociationIBC = Intermediate Bulk ContainerIMDG = International Maritime Dangerous GoodsLogPow = logarithm of the octanol/water partition coefficientMARPOL 73/78 = International Convention for the Prevention of Pollution From Ships,1973 as modified by the Protocol of 1978. ("Marpol" = marine pollution)UN = United Nations

11/24/2015.

No previous validation.

1

00

2Health

Special

Instability/Reactivity

Flammability

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