Rockland Q1 2014 Antibody Newsletter Layout 1rockland-inc.com/uploadedFiles/Marketing/Rockland 2014...

Cancer

Neuroscience

Epigenetics

DNA Ladders

Gel Electrophoresis Markers

TrueBlot® IP/ Western Blotting

AKT

Rockland Immunochemicals, partnering with theNational Cancer Institute’s Center for Cancer Research,has released a series of antibodies that are involved insignal transduction and cancer research. The goal of thiscollaboration is to combine basic and applied researchprinciples, working with life science researchers todevelop better bioreagents that will facilitate biomedicalresearch. These reagents assist in the understanding ofcell function as it relates to cancer management.

* NCI Validated Antibodies are marked with an asterisk.

800.656.7625 www.rockland-inc.com [email protected]

Volume 1 • Version 1

PRODUCT NAME CATALOG# APPLICATIONS SIZE

*Anti-AGAP4 (RABBIT) Antibody 200-401-P07 WB, E 100 µg

*Anti-MMTV (RABBIT) Antibody 100-401-P12 WB, E 100 µL

*Anti-MMTV (RABBIT) Antibody 100-401-P13 WB, E 100 µL

*Anti-ABCB1 (Rabbit) 600-401-967 IHC, WB, E 100 µg

*Anti-ABCB8 (Rabbit) Antibody 100-401-p00 WB, E 100 µL

Anti-AKT pan (Mouse Mab) 200-301-401 WB, IP, E 100 µg

Anti-AKT pan (Rabbit) 100-401-401 IHC, IF, WB, IP, E 200 µL

Anti-AKT pan (Sheep) 600-601-401 WB, IP, E 100 µg

Anti-AKT Phospho pS473 (Rabbit) 600-401-268 IHC, IF, WB, IP, E 100 µg

Anti-AKT Phospho pT308 (Rabbit) 600-401-269 IHC, WB, IP, E 100 µg

Anti-AKT1 isoform specific (Mouse Mab) 200-301-I51 IHC, WB, E 100 µg

Anti-AKT2 (Rabbit) 600-401-425 WB, IP, E 100 µg

Anti-AKT2 isoform specific (Mouse Mab) 200-501-E71 IHC, WB, E 100 µg

Anti-AKT3 isoform specific (Mouse Mab) 200-301-E75 IHC, WB, E 100 µg

Anti-Angiopoietin 1 (Rabbit) 100-401-403 IHC, WB, E 200 µL

Anti-Angiopoietin 2 (Rabbit) 100-401-402 WB, E 200 µL

Anti-ATG13 (Rabbit) 600-401-C50 WB, E 100 µg

Anti-ATG13 Phospho S318 (Rabbit) 600-401-C49 WB, E 100 µg

Anti-ATM Phospho Monoclonal Antibody S1981 200-301-400 IHC, IF, WB, IP, E 100 µg

Anti-ATM Protein Kinase S1981 (Rabbit) 600-401-398 IF, WB, IP, E 100 µg

Anti-Aurora B Phospho T232 (Rabbit) 600-401-677 IF, WB, E 100 µg

Anti-BrdU (RABBIT) Antibody 600-401-C29 IF, IHC, IP, WB, E 100 µg

Anti-BrdU Monclonal Antibody 200-301-H50 IF, IHC, WB, E 100 µg

*Anti-c-MET Phospho pY1349/ pY1356 (Rabbit) 600-401-989 WB, E 100 µg

Anti-Cdk9 (Rabbit) 100-401-167 IHC, IP, WB, E 100 µL

Anti-Collagen Type I (Rabbit) 600-401-103-0.1 IHC, IP, WB, E 100 µg

Anti-Collagen Type II (Rabbit) 600-401-104-0.1 IHC, IP, WB, E 100 µg

Anti-Collagen Type III (Rabbit) 600-401-105-0.1 IHC, IP, WB, E 100 µg

Anti-Collagen Type IV (Rabbit) 600-401-106-0.1 IHC, IP, WB, E 100 µg

Anti-Collagen Type V (Rabbit) 600-401-107-0.1 IHC, IP, WB, E 100 µg

Anti-Cyclin D1 (Rabbit) 100-401-153 WB, IP, E 100 µL

Anti-DNA PKcs Phospho T2609 (Rabbit) 600-401-494 WB, E 100 µg

Anti-Fibronectin (Rabbit) 600-401-117-0.1 IHC, IP, WB, E 100 µg

Anti-Glycogen Synthase Phospho pS640 (Rabbit) 600-401-415 IHC, WB, E 100 µg

Anti-GSK3 alpha (Rabbit) 600-401-444 WB, IP, E 100 µg

Anti-GSK3 alpha Phospho pS21 (Rabbit) 600-401-429 WB, IP, E 100 µg

Anti-GSK3 beta pS9 (Rabbit) 600-401-424 IHC, WB, IP, E 100 µg

Anti-HER3 (Rabbit) 200-401-205 IP, WB, E 100 µg

*Anti-HSP 90 K294 (Rabbit) 600-401-981 IHC, WB, E 100 µg

Anti-IdU Monoclonal Antibody 200-301-H51 WB, E 100 µg

*Anti-IFN induced Gtpases Like Protein (Rabbit) Antibody 100-401-N95 WB, E 100 µL

Anti-IKB alpha C-terminal (Rabbit) 100-4167C WB, IP, E 100 µL

Anti-IKB beta (Rabbit) 100-4186 WB, IP, E 100 µL

Anti-IKK alpha (Rabbit) 100-401-219 WB, IP, E 100 µL

Anti-IKK beta (Rabbit) 100-401-220 WB, IP, E 100 µL

Anti-IKKe Phospho pT501 (Rabbit) 600-401-267 WB, IP, E 100 µg

Anti-IL-1 beta (Rabbit) 209-401-301 IHC, IP, WB, E 1 mg

Anti-IL-6 (Rabbit) 109-401-310 IHC, IP, WB, E 1 mL

Rb anti-AKT Ms anti-AKT pS473

Rb anti-AKT pS473 Ms anti-AKT pT308

Rb Mab anti-BAX Ms anti-BrdU

Rb anti-GSK3 alpha Rb anti-GSK3 beta pS9

Rb anti-MDM2 Rb anti-mTOR

Rb anti-mTOR pS2448 Rb anti-NFκB cRel

Cancer Antibodies



Anti-IL-6 (Rabbit) 209-401-310 IHC, WB, E 100 µg

Anti-IL-10 (Rabbit) 109-401-312 IHC, WB, E 1 mL

*Anti-L1/ORF2 (Chicken) Antibody 200-901-P11 E 100 µg

Anti-Lysozyme (Rabbit) 200-401-072 IHC, IP, WB, E 100 µg

Anti-Mcl-1 (Rabbit) 600-401-394 WB, E 100 µg

Anti-MDM2 (Rabbit) 600-401-927 WB, IP, E 100 µg

Anti-MDM2 Phospho pS185 (Rabbit) 600-401-423 WB, IP, E 100 µg

Anti-Mesothelin (Mouse Mab) 200-301-A88 IHC, WB, E 100 µg

Anti-mTOR (Rabbit) 600-401-897 WB, IP, E 100 µg

Anti-mTOR Phospho pS2448 (Rabbit) 600-401-422 IHC, WB, IP, E 100 µg

Anti-Myosin (Rabbit) 600-401-938 IP, WB, E 100 µg

Anti-Myosin phospho pS19 / pS20 (Rabbit) 600-401-416 IHC, IP, WB, E 100 µg

Anti-Nag-1 (Rabbit) 610-401-B87 WB, E 100 µg

Anti-NFκB p105 (Rabbit) 100-4184 WB, E 100 µL

Anti-NFκB p65 NLS specific (Rabbit) 600-401-271 IHC, IF, WB, IP, E 100 µg

Anti-NFκB cRel (Rabbit) 100-4166 WB, GS, IP, E 100 µL

Anti-NFκB p50 (NFKB1) (Rabbit) 100-4164 IHC, WB, GS, IP, E 100 µL

Anti-NFκB p52 (Rabbit) 100-4185 WB, GS, IP, E 100 µL

Anti-NFκB p65 (Rel A) N-TERMINAL SPECIFIC (Rabbit) 100-4165N WB, IP, E 100 µL

Anti-NFκB p65 (Rel A) (Mouse Mab) 200-301-065 IHC, IF, WB, IP, E 100 µg

Anti-NFκB p65 (Rel A) (Rabbit) 100-4165 IHC, WB, GS, IP, E 100 µL

Anti-NFκB p65 (Rel A) Phospho pS276 (Rabbit) 100-401-264 WB, E 100 µL

Anti-NFκB p65 (Rel A) Phospho pS529 (Rabbit) 100-401-266 WB, IP, E 100 µL

*Anti-NFκB p65 (Rel A) Phospho pS536 (Rabbit) 600-401-265 IHC, WB, IP, E 100 µg

Anti-Osteopontin (Rabbit) 100-401-404 IHC, IP, WB, E 200 µL

Anti-p21 (Mouse Mab) 200-301-171 WB, IP, E 100 µL

Anti-p27 (Rabbit) 100-401-172 WB, IP, E 100 µL

Anti-p53 (Mouse Mab) 200-301-174 ChIP, IF, IHC, WB, IP, E 100 µg

Anti-p70 S6 Kinase (Rabbit) 600-401-942 WB, IP, E 100 µg

*Anti-Pdcd4 (Rabbit) 600-401-965 IHC, WB, IP, E 100 µg

*Anti-Pdcd4 Phospho pS457 (Mouse Mab) 200-301-964 IHC, WB, IP, E 100 µg

Anti PDK1 (Rabbit) 600-401-411 WB, IP, E 100 µg

Anti-PPAR alpha (Rabbit) 600-401-421 IHC, WB, E 100 µg

Anti-PPAR delta (Rabbit) 600-401-420 WB, E 100 µg

Anti-PTEN (Rabbit) 600-401-A90 WB, IP, E 100 µg

Anti-PTEN-P1 Phospho pS29/pT32 (Rabbit) 600-401-859 WB, IP, E 100 µg

*Anti-Setdb2 (RABBIT) Antibody 200-401-N99 WB, E 100 µg

Anti-SFRP1 (Rabbit) 600-401-475 IF, IHC, WB, E 100 µg

*Anti-SMAD3 (Rabbit) 600-401-920 IHC, WB, E 100 µg

*Anti-SMAD3 phospho pS423 / pS425 (Rabbit) 600-401-919 IHC, WB, E 100 µg

Anti-SUMO (Rabbit) 200-401-428 WB, E 500 µg

Anti-Telomerase (Rabbit) 600-401-252 IF, IHC, IP, WB, E 100 µg

Anti-TGF beta 1 (Rabbit) 600-401-432 IF, WB, E 100 µg

Anti-TNF alpha (Rabbit) 209-401-306 IHC, WB, E 1 mg

Anti-UBIQUITIN (Rabbit) 200-401-431 WB, IP, E 500 µg

Rb anti-Osteopontin Rb anti-SMAD3

Rb anti-NFkB p65 pS536 Ms anti-p21

Rb anti-p27 Ms anti-p53

Rb anti-p70 S6 Rb anti-Pdcd4

Rb anti-NFkB p65 pS276 Rb anti-NFkB p65 pS529

Rb anti-NFkB p50 Rb anti-NFkB p65


Catalog# 600-401-253 Size: 100 ug

Tested Applications: IF Microscopy, Immunohistochemistry, Western Blot, ELISA

Beta amyloid antibody shows a 40-50 kD band consistent with ahigher MW precursor is detected in western blot using whole tissueextracts from mouse brain.

Reactivity: human, mouse

Anti-Beta Amyloid Antibody

Anti-Beta Amyloid was affinity purified from monospecific antiserum by immunoaffinity chromatography. Antibodyis directed against the amino terminal end of beta amyloid. Cross reactivity with beta amyloid from other species islikely but has not been determined.


Anti-ABCA4 (Rim Protein) (Mouse Mab) 200-301-D05 IHC, WB, E 100 µL

Anti-Alpha Synuclein (Mouse Mab) 500-301-E56 IF, WB, E 100 µL

Anti-Amyloid Oligomers (A11) (Rabbit) 200-401-E88 IHC, IP, WB, E 100 µL

Anti-AP2A (Goat) 600-101-287 WB, IP, E 100 µg

Anti-AP3D1 (Goat) 600-101-290 WB, IP, E 100 µg

Anti-Apolipoprotein A-1 (Human) (Goat) 600-101-109 IHC, WB, E 1.0 mg

Anti-Apolipoprotein B (Human) (Goat) 600-101-111 IHC, WB, E 1.0 mg

Anti-Apolipoprotein E (Human) (Goat) 600-101-197 IHC, WB, E 500 µg

Anti-Apolipoprotein J (Goat) 600-101-198 IHC, IP, WB, E 500 µg

Anti-Beta Amyloid (Rabbit) 600-401-253 IHC, WB, IP, E 100 µg

Anti-Beta Amyloid protein 40 (Rabbit) 600-401-J10 IF, IHC, WB, E 50 µg

Anti-Beta Amyloid pyro E11 (Rabbit) 600-401-J13 IF, IHC, WB, E 50 µg

Anti-Beta Amyloid pyro E3 (Rabbit) 600-401-J14 IF, IHC, WB, E 50 µg

Anti-Calretinin (Sheep) 200-601-D13 IF, IHC, WB, E 100 µL

Anti-CaM Kinase II (Rabbit) 600-401-873 WB, E 100 µg

Anti-CaM Kinase II (Mouse Mab) 200-301-F00 IF, IHC, IP, WB, E 100 µL

Anti-CaM Kinase II Phospho T286 (Rabbit) 612-401-C71 WB, E 100 µL

Anti-CaM Kinase II Phospho T305 (Rabbit) 112-401-C72 WB, E 100 µL

Anti-Catalase (Rabbit) 200-401-051 WB, E 100 µL

Anti-ChAT (Choline Acetyltransferase) (Rabbit) 100-401-D16 IHC, WB, E 100 µL

Anti-COX-2 (Rabbit) 100-401-226 WB, IP, E 100 µL

Anti-Dab1 (400-555) (Mouse) (Rabbit) 100-401-225 IHC, WB, IP, E 100 µL

Anti-DELTA-4 (Rabbit) 600-401-696 IHC, WB, IP, E 100 µg

Anti-Dopamine Transporter (Rabbit) 600-401-C75 IHC, WB, E 100 µL

Anti-EGR-1 (Rabbit) 600-401-693 IHC, WB, IP, E 100 µg

Anti-FREQUENIN (RABBIT) 600-401-216 IF, WB, IP, E 100 µg

Anti-GABA(A) Receptor beta 3 pS408/pS409 (Rabbit) 612-401-D51 WB, E 100 µL

Gt anti-AP3D1 Gt anti-Apolipoprotein A1

Gt anti-Apolipoprotein B Rb anti-BACE

Rb anti-COX-2 Rb anti-Dab1

Rb anti DELTA-4 Rb anti-EGR-1

Neuroscience



Anti-GABA(A) Receptor delta (Rabbit) 600-401-C80 IHC, IP, WB, E 100 µL

Anti-GABA(B) Receptor 2 Phospho pS783 (Rabbit) 612-401-D53 IF, WB, E 100 µL

Anti-GFP (Mouse Mab) 600-301-215 IF, IHC, IP, WB, E 1.0 mg

Anti-Gli-1 (Rabbit) 100-401-223 IHC, WB, E 100 µL

Anti-Gli-2 (Rabbit) 600-401-845 IHC, WB, IP, E 100 µg

Anti-Gli-3 (Rabbit) 600-401-694 IHC, WB, E 100 µg

Anti-GluR2 Phospho S880 (Rabbit) Antibody 612-401-D64 WB, E 100 µL

Anti-Heat Shock Protein 27 (HSP27) (Mouse Mab) 200-301-243 IHC, WB, IP, E 100 µg

Anti-Huntington Phospho pS421(Rabbit) 600-401-433 IHC, WB, IP, E 100 µg

Anti-Jagged-1 (Rabbit) 200-401-698 IHC, IF, WB, IP, E 500 µg

Anti-Leptin (Rabbit) 210-401-320 IHC, WB, E 100 µg

Anti-MBP (Myelin Basic Protein) (Chicken) 200-901-D81 IF, WB, E 100 µg

Anti-Mouse Gli-2 (Rabbit) 600-401-695 IHC, WB, E 100 µg

Anti-Nestin (Rabbit) 600-401-417 WB, IP, E 100 µg

Anti-Neuraminidase2 (Neu2) (Rabbit) 600-401-850 WB, IP, E 100 µg

Anti-Neurofilament H (Mouse Mab) 200-301-D82 IF, WB, E 100 µL

Anti-Neurofilament L (Mouse Mab) 200-301-D83 IF, WB, E 100 µL

Anti-Neurofilament M (Mouse Mab) 212-301-D84 IF, WB, E 100 µL

Anti-Nitrotyrosine (Mouse Mab) 200-301-A98 IHC, WB, IP, E 100 µg

Anti-NogoA (Rabbit) 600-401-C01 IHC, WB, E 100 µg

Anti-NOTCH 1 (Cleaved N terminal) (Rabbit) 100-401-407 WB, IP, E 200 µL

Anti-NOTCH 1 (intra) (Human specific) (Rabbit) 100-401-405 WB, IP, E 200 µL

Anti-NOTCH 2 (Cleaved N terminal) (Rabbit) 100-401-408 WB, IP, E 200 µL

Anti-NOTCH 2 (intra) (Human specific) (Rabbit) 100-401-406 WB, IP, E 200 µL

Anti-PACRG (Rabbit) 600-401-474 WB, E 100 µg

Anti-Potassium Chloride Cotransporter pS940 (Rabbit) 612-401-E15 WB, E 100 µL

Anti-ROBO-1 (Rabbit) 600-401-692 IHC, WB, E 100 µg

Anti-S100 Protein (Rabbit) 200-401-874 IHC, WB, E 500 µg

*Anti-Selenoprotein W (Rabbit) 600-401-A29 WB, IP, E 100 µg

Anti-SH3GL1 (Mouse Mab) 200-301-G41 IF, IHC, IP, WB, E 100 µL

Anti-SLIT-1 (Rabbit) 600-401-860 WB, IP, E 100 µg



Anti-Superoxide Dismutase (Rabbit) 200-4191-0100 WB, E 100 µg

Anti-Synapsin I (Rabbit) 100-401-C92 WB, E 50 µL

*Anti-Tamalin (Rabbit) 600-401-988 WB, E 100 µg

Anti-Tau Monoclonal Antibody 200-C01-B33 IHC, WB, IP, E 100 µL

Anti-Tau phospho S416 (Rabbit) 612-401-E42 WB, E 100 µL

Anti-Tetanus Toxin fragment C (TTFC) (Rabbit) 100-401-894 WB, IP, E 100 µL

Anti-TrkB (Rabbit) 612-401-E47 WB, E 100 µL

Anti-Zic-1 (Mouse/Human) (Rabbit) 200-401-159 WB, IP, E 100 µg

Anti-ZIC-2 (Rabbit) 600-401-848 WB, IP, E 100 µg

Rb anti-Jagged Rb anti-ROBO-1

Rb anti-S100 Protein Rb Mab anti-Tau

Rb anti-Gli-3 Rb anti-Huntington pS421

Rb anti-Frequenin Rb anti-Gli-2


Rb anti-ABCA4 Rb anti-NogoA

Rb anti-Tamalin Sh anti-Calretinin

Epigenetics


Anti-14-3-3 (Rabbit) 612-401-D01 WB, E 100 µg

Anti-APRMT6 (Rabbit) 100-401-I04 WB, E 200 µL

Anti-ATDC Ac-K116 (Rabbit) 600-401-B16 WB, E 100 µg

Anti-ATF6 (Mouse Mab) 200-301-H56 WB, E 100 µg

Anti-Blimp1 / PRDM1 (Rabbit) 600-401-B52 WB, E 100 µg

Anti-BRRN-1 (Rabbit) 600-401-682 WB, E 100 µg

*Anti-CENP-Q (Rabbit) 200-401-B48 WB, E 100 µg

Anti-Co-Rest/RCOR1 (Mouse Mab) 200-301-F16 IHC, IP, WB 100 µg

Anti-CREB-1 (Rabbit) 100-401-195 GS, WB, E 100 mL

Anti-CTCF (Rabbit) 600-401-C42 WB, E 100 µg

Anti-Dicer (Rabbit) 200-401-H68 IF, WB 100 µg

Anti-Dnmt1 (Mouse Mab) 200-301-H69 IHC, WB, E 100 µg

Anti-Dnmt3a (Mouse Mab) 200-301-H70 IF, WB, E 100 µg

Anti-Dnmt3b (Mouse Mab) 200-301-H71 IF, IP, WB, E 100 µg

Anti-Dnmt3L (Rabbit) 600-401-454 IP, WB, E 100 µg

Anti-Dnmt3L (Mouse Mab) 209-301-F22 IF, IHC, IP, WB, E 100 µg

Anti-EZH1 (Rabbit) 200-401-H75 WB, E 100 µg

Anti-HB9 (Rabbit) 200-401-H78 IHC, WB, E 100 µg

Anti-HDAC1 (Rabbit) 600-401-879 IHC, WB, E 100 µg

Anti-HDAC1 (Rabbit) 600-401-J16 IF, IHC, WB, E 100 µg


Anti-HDAC4 (Rabbit) 600-401-J20 IHC, WB, E 100 µg

Anti-HDAC5 (Rabbit) 600-401-J21 WB, E 100 µg

Anti-HDAC6 (Rabbit) Antibody 600-401-J39 WB, E 100 µg


Anti-HDAC9 (Rabbit) Antibody 600-401-J73 WB, E 110 mL

Anti-HDAC10 (Rabbit) Antibody 600-401-J76 WB, E 100 µg

Rb anti-H3 ac-Lys18 Rb anti-H3 ac-Lys4

Rb anti-H3 Me2-Lys18 Rb anti-H3 Me2-Lys36

Rb anti-HDAC1 Rb anti-H3 K4ac


Catalog# 600-401-I57 Size: 50 ug

Tested Applications: ChIP, IF Microscopy, Immunohistochemistry,Western Blot, ELISA

Antibody is specific for mono-methylated Lysine 4 of histone H3. Doesnot recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9.

Reactivity: human, mouse, C. elegans, rat, chicken, Xenopus,Drosophila, plant

Anti-Histone H3 (mono methyl K4) Antibody

Anti-Histone H3 [Monomethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinitychromatography. This antibody reacts with human Histone H3.2. This gene is intronless and encodes a member of thehistone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.



Anti-Histone H2AvD pS137 (Rabbit) 600-401-914 WB, E 100 µg

Anti-Histone H3 (Rabbit) 100-401-E81 WB, E 100 µL

Anti-Histone H3 K18ac (Rabbit) 600-401-I81 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 K18me1 (Rabbit) 600-401-I78 ChIP, IF, IHC, WB, E, Dot 50 µg




Anti-Histone H3 K27ac (Rabbit) Antibody 600-401-K00 WB, Dot 50 µg

Anti-Histone H3 K27Me3 (Rabbit) 600-401-I95 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 K27me3/pS28 (Rabbit) 600-401-I84 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 K27 methyl (Rabbit) Antibody 200-401-M74 WB 50 µg

Anti-Histone H3 K27 methyl (Rabbit) Antibody 600-401-M75 WB 100 µg

Anti-Histone H3 K36ac (Rabbit) 600-401-I89 WB, E 50 µg



Anti-Histone H3 K36me3 (Rabbit) 600-401-I88 ChIP, IF, IHC, WB, Dot 50 µg

Anti-Histone H3 K37me1 (Rabbit) 600-401-I90 WB, E 50 µg


Anti-Histone H3 K37me3 (Rabbit) 600-401-I92 ChIP, WB 50 µg



Anti-Histone H3 K4me1/pT6 (Rabbit) 600-401-I61 ChIP, IF, IHC, WB, E, Dot 50 µg




Anti-Histone H3 K4me3/K9ac (Rabbit) Antibody 600-401-J98 WB, Dot 50 µg




Anti-Histone H3 K79-Me1 (Rabbit) Antibody 600-401-P10 ChIP, IF, WB, Dot 50 µg

Anti-Histone H3 K9Ac/pS10 (Rabbit) Antibody 600-401-P08 IF, WB, Dot 50 µg


Anti-Histone H3 K9ac/K14ac (Rabbit) 600-401-I73 ChIP, IF, IHC, WB, E, Dot 50 µg







Anti-Histone H3 [methyl Lys27] (Rabbit) Antibody 200-401-M74 WB 50 µg

Anti-Histone H3 [methyl Lys27] (Rabbit) Antibody 600-401-M75 WB 50 µg

Anti-Histone H3 pS10 (Rabbit) 600-401-I74 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 pS10/pT11 (Rabbit) 600-401-I75 ChIP, IF, IHC, WB, E, Dot 50 µg


Anti-Histone H3 pT11 (Rabbit) 600-401-I76 ChIP, IF, IHC, WB, E, Dot 50 µg


Rb anti-H3 pS10 Rb anti-H3 pS28

Rb ant-H3 pT3 Me2-Lys4 Rb anti-H3 pT3 Me1-Lys4

Rb anti-H3 pT11 Rb anti-H3 pT11/pS10

Rb anti-H3 pT6 Rb anti-H3 pT6-Me2-Lys9




Anti-Histone H3 pT3/K4ac (Rabbit) 600-401-I56 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 pT3/K4me1 (Rabbit) 600-401-I54 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 pT3/R2-Me2s (Rabbit) Antibody 600-401-p14 IF, WB, Dot 50 µg

Anti-Histone H3 pT6 (Rabbit) 600-401-I63 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 R17me2a (Rabbit) 600-401-I77 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 R2me1 (Rabbit) 600-401-I52 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 R2me2a (Rabbit) 600-401-I53 ICC, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 R2me2s/K4me2 (Rabbit) 600-401-I85 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 R8me2a (Rabbit) 600-401-I67 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H3 R8me2s (Rabbit) 600-401-I68 ChIP, WB, Dot 50 µg

Anti-Histone H3 (Rabbit) Antibody 100-401-E81 WB, E 100 µL

Anti-Histone H4 [ac Lys12] (Rabbit) Antibody 600-401-P06 IF, WB, Dot 50 µg

Anti-Histone H4 K16ac (Rabbit) 600-401-J00 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H4 K20me1 (Rabbit) 600-401-J01 ChIP, IF, IHC, WB, E, Dot 50 µg

Anti-Histone H4 K20me2 (Rabbit) 600-401-J02 ChIP, IF, IHC, WB 100 µg

Anti-Histone H4 K5ac (Rabbit) 600-401-I98 ChIP, IHC, WB, E, Dot 50 µg



Anti-Histone H4 R3me1 (Rabbit) 600-401-I97 ChIP, IF, IHC, WB, E, Dot 50 µg

*Anti-HMGN pS20/pS24 (Rabbit) 600-401-898 IHC, WB, E 100 µg

Anti-KLF4 (Rabbit) 200-401-H88 F, IHC, WB, E 100 µg

Anti-Lysine Acetylated (Rabbit) 600-401-939 IHC, IP, WB, E 100 µg

Anti-Lysine Methylated (Rabbit) 600-401-940 IHC, WB, E 100 µg

Anti-MBD3 (Mouse Mab) 200-301-H92 WB, E 100 µg

Anti-MITF (Mouse Mab) 200-301-H93 WB, E 100 µg

Anti-MyoD (Mouse Mab) 200-301-H94 IF, IP, WB 100 µg

*Anti-NCOA3 (Rabbit) 600-401-A40 IP, WB, E 100 µg

Anti-NFATc1 (Rabbit) 200-401-H95 WB, E 100 µg

Anti-Nur77 (Rabbit) 200-401-H99 WB, E 100 µg

Anti-p28 ING5 (Goat) 600-101-285 WB, E 100 µg




Anti-p73 (Mouse Mab) 200-301-I02 ChIP, IHC, WB 100 µg

Anti-PARP (Mouse Mab) 200-301-I03 F, WB, E 100 µg

Anti-PCNA (Rabbit) 600-401-968 WB, E 100 µg

Anti-RING1B (Goat) 600-101-292 IF, WB, E 100 µg

Anti-SIRT5 (Rabbit) 200-401-I14 WB, E 100 µg

Anti-STING (Rabbit) 600-401-I20 WB, E 100 µg

Anti-Tankyrase (Mouse Mab) 200-301-I21 WB, E 100 µg

Anti-TET2 (Rabbit) Antibody 100-401-N93 WB, E 100 µL

Anti-TET3 (Rabbit) Antibody 100-401-N94 WB, E 100 µL


Rb anti-HMGN pS20/24 Rb anti-H4 K5Ac

Rb anti-H4 R3me1 Rb anti-RING1B

Histone H3 Antibody Histone H3 K27 Methyl Antibody

p73 Antibody TET3 Antibody

Rb anti-H4 K20me2 Rb anti-H4 pS1

Epigenetics



G.E.M.: Gel Electrophoresis MarkersRockland’s Gel Electrophoresis Marker (GEM) product line covers a variety ofprestained protein standards and DNA ladders for electrophoresis applications.Protein standards suitable for SDS- PAGE and western blot analysis, and DNAladders for use in agarose gels, are provided as ready-to use markers. All GEMproducts display an array of bands to eliminate molecular weight size ambiguity,while reducing the need for hands-on work. These broad range markers areideal for use in a variety of gels with a variety of running buffers.

G.E.M. Broad Range DNA LaddersDiamond Ready-to-Use DNA Ladders contain a unique combination of PCR products and a number of proprietaryplasmids digested with appropriate restriction enzymes to yield various fragments, suitable for use as molecular weightstandards for agarose gel electrophoresis applications.

Sapphire: Blue protein standard.

Ruby: Two-color protein standard.

Opal: Three-color protein standards for a wide variety of molecular weights.

Diamond: Provides a reference point for UV viewing. Contain a combination of orange G, xylene cyanol FF and bromophenol blue as tracking dyes.

PRODUCT NAME CATALOG# SIZE

Ruby Prestained Protein Standard 10-175kDa MB-208-0500 500 µL

Opal Prestained Protein Standard 10-180kDa MB-209-0500 500 µL

Opal Prestained Protein Standard 10-245kDa MB-210-0500 500 µL

Opal Prestained Protein Standard 3.5-245kDa MB-211-0500 500 µL

Sapphire Prestained Protein Standard 10-180kDa MB-212-0500 500 µL


Diamond Ready-to-Use DNA Ladder 100-1500bp MB-202-0500 500 µL


Diamond Ready-to-Use DNA Ladder 250-10Kbp MB-204-0500 500 µL

Diamond Ready-to-Use DNA Ladder 100-10Kbp MB-205-0500 500 µL


Diamond Ready-to-Use DNA Ladder 250-25kbp MB-207-0500 500 µL


TrueBlot®: Clear Detection of Blotted Bands

Referenced in over 300 publications!

TrueBlot® preferentially detects the native disulfide form of mouse IgG or rabbit IgG,

making it ideal for IP and Western Blot protocols involving immunoblotting of

immunoprecipitated proteins. TrueBlot® reduces interference by the ~55 kDa heavy

and ~23 kDa light chains of the immunoprecipitating antibody in IP/immunoblotting

applications. TrueBlot® is also ideal for studying post-translational protein

modifi cations, including phosphorylation, or protein-protein interactions.

Fluorescent TrueBlot®

Fluorescent TrueBlot®, a new format of our popular

TrueBlot® product line, combines the power and specificity

of the original products with the versatility of fluorescent

and near infra-red dyes. Conjugating highly optimized

TrueBlot® reagents to a full spectrum of fluorescent labels,

results in multipurpose reporter molecules that can be

used in a variety of immunoassays.


TrueBlot® Anti-Rabbit Ig IP Beads 00-8800-25 2.5 mL

TrueBlot® Anti-Mouse Ig IP Beads 00-8811-25 2.5 mL

TrueBlot® Anti-Goat Ig IP Beads 00-8844-25 2.5 mL

Goat TrueBlot®: Anti-Goat IgG HRP 18-8814-31 50 µL

Goat TrueBlot®: Anti-Goat IgG HRP 18-8814-33 200 µL

Rabbit TrueBlot®: Anti-Rabbit IgG HRP 18-8816-31 50 µL

Rabbit TrueBlot®: Anti-Rabbit IgG HRP 18-8816-33 200 µL

Mouse TrueBlot® ULTRA: Anti-Mouse Ig HRP 18-8817-30 20 µL



Goat TrueBlot® Set (with IP beads) 88-1488-31 1 Set

Rabbit TrueBlot® Set (with IP beads) 88-1688-31 1 Set

Mouse TrueBlot® Set (with IP beads) 88-7788-31 1 Set

Goat TrueBlot® Western Blot Kit 88-8884-31 1 Kit

Rabbit TrueBlot® Western Blot Kit 88-8886-31 1 Kit

Mouse TrueBlot® Western Blot Kit 88-8887-31 1 Kit


Fluorescent TrueBlot®: Anti-Rabbit IgG Fluorescein 18-0216-32 100 µL

Fluorescent TrueBlot®: Anti-Mouse IgG Fluorescein 18-0217-32 100 µL

Fluorescent TrueBlot®: Anti-Rabbit IgG IRDye800® 18-3216-32 100 µL

Fluorescent TrueBlot®: Anti-Mouse IgG IRDye800® 18-3217-32 100 µL

Fluorescent TrueBlot®: Anti-Rabbit IgG DyLight™ 680 18-4416-32 100 µL

Fluorescent TrueBlot®: Anti-Mouse IgG DyLight™ 680 18-4417-32 100 µL


AKT Isoform-Specific Monoclonal Antibodies

Western Blot of AKT isoform specific antibodies: 50 ng of

recombinant GST-AKT1, -AKT2 and -AKT3 proteins were

separated by SDS PAGE (4-20% gel) and transferred onto

0.2 μm nitrocellulose. The membrane was blocked with

Blocking Buffer (p/n MB-070) for 1 h at room temperature.

Mouse anti-AKT1 clone 14E5A2B2 H9/E5 (p/n 200-301-

I51), Rat anti-AKT2 clone 16G11A7 (p/n 200-501-E71) and

Mouse anti-AKT3 clone 25F6F6D8 (p/n 200-301-E75) were

applied at a dilution of 1 μm/mL in Blocking Buffer and

incubated for 16 h at 4°C. Rabbit anti-Mouse IgG HRP (p/n

610-403-C46) or Goat anti-Rat IgG HRP (p/n 612-103-120)

secondary antibodies were applied at 0.05 μg/mL in

Blocking Buffer and incubated for 1 h at room temperature

followed by detection with FemtoMax™ chemiluminescent

reagent (p/n FEMTOMAX-110). Arrowheads indicate the

position of each AKT isoform in blots.

AKT1 Antibody APC Conjugated200-326-J34

AKT1 Antibody PE Conjugated200-308-J34

AKT (Protein Kinase B or PKB), is a serine/threonine-specif c protein kinase with known key involvement inglucose metabolism, apoptosis, cell proliferation, transcription and cell migration. More recently, AKT activationis associated with tumor cell survival, proliferation, and invasiveness. AKT inhibitors are the subject of ongoingclinical trails for the treatment of cancer. Isoforms of AKT are found to be distinct with regard to tissueexpression, pathway activation and inhibitor sensitivity. Rockland has created a panel of monoclonal antibodiesto AKT isoforms suitable for probing lysates and pharmacodynamic studies.

AKT Isoform-Specific Monoclonal Antibodies

Anti-AKT Monoclonal Antibody

Anti-AKT1 Monoclonal Antibody




Unconjugated BIOTIN APC PE FITC

200-301-401100 µg

200-301-I51100 µg

200-301-J34100 µg

200-501-E71100 µg

200-301-E75100 µg

200-306-40150 µg

200-306-I5150 µg

200-306-J3450 µg

200-506-E7150 µg

200-306-E7550 µg

200-326-40150 µg

200-326-I5150 µg

200-326-J3450 µg

200-526-E7150 µg

200-326-E7550 µg

200-308-40150 µg

200-308-I5150 µg

200-308-J3450 µg

200-508-E7150 µg

200-308-E7550 µg

200-302-40150 µg

200-302-J3450 µg

200-302-J3450 µg

200-502-E7150 µg

200-302-E7550 µg

LEFT PANEL: Plate was coated with monoclonal anti-AKT1

antibody (capture antibody) followed by incubation with

activated recombinant AKT1, AKT2, AKT3 proteins. Binding was

detected with biotinylated mAb anti-AKT pS473.

RIGHT PANEL: Plate was coated with monoclonal anti-AKT1

antibody (capture antibody) followed by incubation with

recombinant AKT1 or phosphorylated AKT1 proteins. Binding was

detected with biotinylated monoclonal anti-AKT pS473.

SUMMARY: Anti-AKT1 monoclonal antibody is specific to recombinant AKT1 protein and not isoform 2 and 3. Anti-AKT1 isoform specific antibody

and anti-AKT-pS473 (p/n 200-301-268) are suitable antibody pairs for pharmacodynamic assays. Unpublished PD assay data not shown.

Blocking Buffers • Cancer Research • Epitope Tags • Monoclonal Antibody Production • NFkB Pathway • Phospho Specific Antibodies • Secondary Antibodies

PO Box 326Gilbertsville, PA 19525

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