Roberto Chignola - profs.scienze.univr.itprofs.sci.univr.it/~chignola/CV_eng.pdf · Then I focused...

Roberto ChignolaDepartment of BiotechnologyUniversity of VeronaStrada Le Grazie 15 – CV1I – 37134, Verona (Italy)

Phone: (+39) 045-8027953Fax: (+39) 045-8027929Email: [email protected]: http://profs.sci.univr.it/∼chignola

Personal informations

Birthdate: March 27th, 1966Birthplace: Verona, Italy

Present position

Associate Professor (as of Dec. 28, 2018)Area: 09 – Industrial and Information EngineeringSector: 09/G2 – BioengineeringSub-sector: ING-IND/34 – Industrial BioengineeringDepartment of Biotecnology, University of Verona

Education

• 1984: High school diploma

• 1989: Degree (Laurea) In Biology, University of Padova. Thesis title: Applicationof flow cytometry to study the endocytosis of proteinaceous toxin vectors: correlationbetween endocytosis and cytotoxicity (thesis advisor Prof. Cesare Montecucco)

• 1991: National qualification as professional biologist

Teaching qualification

• 2017: Habilitation in Applied Physics

• 2017: Habilitation in Bioengineering

Research interests

• Experimental and theoretical oncology

• Experimental and theoretical immunology

• Toxicity analysis of food components and of drugs

• Applied statistics

• Modeling of biological systems

mailto:[email protected]

http://profs.sci.univr.it/$\sim $chignola

Roberto Chignola: Curriculum Vitae 2

Short description

I started my scientific career at the Immunology Institute of the University of Verona where I prepared mythesis work (that I discussed at the University of Padova). I worked in the field of ligand/toxin conjugatesas experimental anti-tumor therapeutics and I was asked to study their intracellular trafficking and theircytotoxic effects on tumor cells grown in three-dimensional cell culture systems (tumor spheroids). At thattime I could learn the basics of biochemical, molecular biology and cell culture techniques, and I becameskilled in the cytotoxicity testing of drugs and in the production of monoclonal antibodies.

Stimulated by continuous discussions with colleagues, however, I started studying the immunopathologyof tumors and of autoimmune diseases. I then used my acquired skills on the cytotoxicity testing of drugsand my knowledge of immunology to cooperate with food scientists in topics such as the toxicity of foodcomponents on the immune system at the gastro-intestinal interface.

Since the very beginning of my research activity, I thought that a quantitative approach could have helpedus improving our knowledge of complex diseases such as cancer. I therefore attended biophysical coursesand I started to work with experts from different disciplines such as Physics, Mathematics, Engineering.I succeeded in modeling the intracellular trafficking of toxins (first half of the ’90s). Then I focused myresearch on the mathematical modeling of tumor growth, keeping in mind that the mathematical/physicalmodeling of biological processes must always proceed in parallel with experimentation. This approach haspermeated all my research activity, up to recent times where my research is mainly devoted to the bottom-up numerical modeling of solid cancers.

Thus, during the course of my studies I have acquired new skills. I can now translate model equations into

laboratory experiments and vice versa, and use basic and advanced statistical methods to analyse data and

grasp biological information.

Post-lauream training courses

• 1989: International course on Molecular mechanisms of intracellular targeting andsorting, University of Trieste and University of Udine in collaboration with the Bio-physics and Molecular Biology Society

• 1990: First national course on Tumor immunology, University of Verona

• 1997: International course on Chaos and noise in biology and medicine, Italian Insti-tute of Philosophy Studies and International School of Biophysics, Ischia (Napoli)

• 1997: National course on Complex systems in biology, School of Pure and AppliedBiophysics, Venezia

• 2008: National course on From genes to models and return, PhD School in Molecularand Biological Sciences, University of Milano

Profession

• 1990 – 1992: Three-years fellowship from the Italian Association for Cancer Research(AIRC). Project title: Production of chimeric toxins with improved trans-membrane translo-cation properties for the synthesis of anti-tumor ligand/antibody toxin conjugates

• 1993: International Cancer Technology Transfer grant from the National Cancer In-stitute (NCI, USA) and the Union Internationale Contre le Cancer (UICC, Switzerland).


Project title: Immunotoxins in cancer treatment: improvement of pharmacologic poten-tial. Supervisor: Dr. Richard J. Youle. Place: National Institutes of Health (Bethesda,USA)

• 1993 – 1994: Fellowship from the Italian Ministry of Health. Project title: Productionof a chimeric gp41/Ricin A chain toxin

• 1994 – 1996: Fellowship from the local health units ULSS25 followed by a contractwith the University of Verona. Project title: Study on the chemico-physical and biolog-ical properties of cytotoxic molecules for the production of novel anti-tumor molecules

• 1996 – 2002: Permanent position as Laboratory Technician, Immunology Institute,University of Verona

• 2000 and 2001: Visiting fellow at the Instituto Gulbenkian de Ciência, Lisboa, Portu-gal

• 2000 – 2001: Research contract with the National Research Council. Project title:Organized fluctuations in complex biological systems. Place: Institute of ElectronicCircuits, Genova

• 2002 – 2014: Associate fellow at the National Institute for Nuclear Physics

• 2002 – 2018: (Dec. 30, 2002 – Dec. 28, 2018) Assistant Professor, Area 06/A2 –General and Clinical Pathology, Sector MED/04 – General Pathology, Depatment ofBiotechnology, University of Verona

Responsibilities

• 2002 – now: I lead the Experimental and Theoretical Immunology and Oncology Lab(launched in 2002 with grants from Fondazione Cariverona) at the Department ofBiotechnology

• 2002 – 2012: I am the co-investigator of several initiatives in the field of biophysicsof cancer (Virtus, VBL, VBL-Rad and now eBON, with grants from the Commission V,INFN)

• 2002 – 2004: Research unit leader, project Physiopathological aspects of the interac-tions between cereal-based foodstuffs and the immune system, granted by the Fon-dazione Cariverona

• 2002 – 2005: Research unit leader, project title Food quality and health, FISR, spon-sored by four Governmental Ministries

• 2005: Member of the scientific commitee, National Meeting BIOSYS 2005, NationalAssociation for Automation

• 2005 – 2015: Member of the teaching/tutoring staff, PhD in Applied Biotechnology,PhD School in Sciences, Engineering and Medicine

• 2006 – 2009: I actively participated in the project Scientific Degrees (Lauree Scien-tifiche) sponsored by the Ministry of Instruction, University and Research. I organizedlessons and practical short courses of applied mathematics for high-school students

• 2007 – 2010: Research unit leader, project Development of models to assess the risks


deriving from the addition of enzymes to cereal flours on human health, granted by theFondazione Cariverona

• 2010: Co-investigator of an HPC (High Performance Computing) project related toVBL/eBON at CINECA (C type grant)

• 2010: Co-investigator of an HPC project related to VBL at CASPUR (standard HPCgrant)

• 2010: Co-investigator, project title New technologies for the “made in Italy”, Ministryof Economic Development (36 months)

• 2011: Co-investigator of an HPC project related to eBON at CASPUR (standard HPCgrant)

• 2011: Local coordinator of the two years PRIN (Projects of Relevant National Interest)project Numerical simulation of tumor spheroids

• 2011: Co-investigator of a two years Joint Project (University/Industry). Project titleStrategies for the development of new vaccines for wheat allergy

• 2014: Local coordinator, project title MERIDIAN (Measuring the Effects of Radiationon Immunity and DIfferentiAtioN), Commission V, INFN

• 2017 – 2019: Principal investigator, project title Slowing down the aggressiveness ofsolid tumors, Basic Research Program, University of Verona

Institutional responsibilities

• 2003 – 2004: Member of the board of experts for the development of a new specialisticacademic course on Molecular and Industrial Biotechnology, University of Verona

• 2004 – 2010: Member of the Student File Commission, bachelor’s and master degreecourses in Biotechnology, University of Verona

• 2005 – 2015: Member of the Teaching Staff Council, PhD in Applied Biotechnologies,PhD School in Science, Engineering and Medicine, University of Verona

• 2005 – 2009: Member of the Biotechnology Department Board, University of Verona

• 2006 – 2009: Active participation to the project Scientific Degrees (Lauree Scientifiche,PLS) sponsored by the Italian Ministry of Education, University and Research (organ-isation of lessons and laboratory activities on mathematics applied to biomedicine forundergraduate students)

• 2010: Member of the Interdipartmental Center of Experimental Research Service, asthe representative of the Department of Biotechnology

• 2017: Member of the committee for the development of a scientific and didactic de-partmental project, national competition “Dipartimenti di Eccellenza" (Department ofExcellence) sponsored by the Italian Ministry of Education, University and Research(positive evaluation)

• 2017 – 2018: Member of the board of experts for the development of a new masterdegree course on Industrial Biotechnology, University of Verona (activated in 2019)

• 2017 – now: Member of the emergency unit of the Department of Biotechnology,


University of Verona (BLSD, fire hazard and rescue certifications)

• 2019 – now: Member of the Quality Assurance Board of the University of Verona asthe representative of the Science and Engineering Macro-area

Teaching

• 1991 – 1995: General Immunology and Immunohematology (first and second yearcourses, respectively), Regional High School for Professional Nurses

• 1995 – 1996: Immunohematology, University Diploma in Nursing Sciences, Facultyof Medicine, University of Verona

• 2002 – 2010: Immunology, Biotechnology Degree Course, Faculty of Sciences, Uni-versity of Verona

• 2003 – 2010: Techniques of Animal Cell Cultures, Biotechnology Degree Course,Faculty of Sciences, University of Verona

• 2008 – 2010: Introduction to Pathology and Oncology, Master Degree in Molecularand Industrial Biotechnology, University of Verona

• 2012 – 2013: Introduction to Pathology and Immunology, Biotechnology DegreeCourse, University of Verona

• 2005 – 2015: advanced courses in Experimental and Theoretical Oncology, AppliedStatistics, PhD Program in Applied Biotechnology, PhD School in Sciences, Engineer-ing and Medicine, University of Verona

• 2015 – now: Statistics, Biotechnology Degree Course, University of Verona

• 2017 – now: Mathematical models in biology/Mathematical and statistical methodsin biology, Applied Mathematics Degree Course, University of Verona

• 2019 – now: Quantitative methods for Biotechnology, Master Degree in Biotechnologyfor bioresources and sustainable development, University of Verona

• 2002 – now: Advisor (with full responsibility) of numerous thesis works (>30), DegreeCourses in Biotechnology

Invited talks at National and International Meetings

• 1990: DNA analysis by flow cytometry, National meeting on Actualities on Colon-Rectum Carcinoma, University of Pisa, Pisa (Italy)

• 1998: Mathematical approaches to complex systems, National meeting of the ItalianNeuroimmunology Association, Chieti (Italy)

• 2001: Order and disorder in natural systems, National meeting on Mathematics andNeurosciences (Neuromat-II), Pavia (Italy)

• 2002: Mathematical modeling of multicellular tumor spheroid growth: implications forthe growth of solid tumors, International meeting on Advances in the use of multicel-lular spheroids in cancer biology and therapy, ISS, Rome (Italy)


• 2002: Toxicological aspects and problems of foodstuffs from genetically modified or-ganisms, National meeting on Foodstuffs and GMO, Verona (Italy)

• 2006: Metabolism and cell population dynamics, Gordon Research Conference onMetabolism and Ecology, Bates College, Portland, Maine (USA)

• 2006: Virtual Biophysics Lab, National meeting on Applications of physics to biologyand medicine, INFN and University of Trieste, Trieste (Italy)

• 2008: Ab initio computational modeling of tumor spheroids, 1st Transatlantic Work-shop on multilevel cancer modeling, Bruxelles (Belgium)

• 2013: From tumor microenvironment dynamics to scaling-laws in oncology, XCIX Na-tional meeting of the Italian Physics Society, Trieste (Italy)

Public conferences

• 2009: On the origins of tumors: when a cell loses control, High School E. Medi, Vil-lafranca (Verona, Italy)

• 2010: The immune system: how we defend ourselves against the attack of evolvingpathogens, High School E. Medi, Villafranca (Verona, Italy)

• 2011: On the origin of tumors: the basis for a personalized therapy, civic center, SanPietro di Lavagno (Verona, Italy)

Scientific societies

• 1997-2001: Member of the Society for Mathematical Biology

• since 2015: Member of the Italian Society of Cancerology and of the European Asso-ciation for Cancer Research

• since 2019: National Bioengineering Group

Awards

• 2006: Award from the National Association for Automation

Patents

• Highly efficient method used for the screening of bioactive molecules. R. Chignola,C. Dalla Pellegrina, C. Tomelleri. University of Verona, Patent number: IT1380835-B(Sep. 13, 2010)

• Multi-layered paticles. F. Zanoni, G. Zoccatelli, M. Vakarelova, R. Chignola. USProvisional Patent Application 62/769,642; Patent 067641-P0001A RDG (2018)


Publications

(most recent items come first)

Refereed papers in scientific journals

1. C. Cavallini, M. Galasso, E. Dalla Pozza, R. Chignola, O. Lovato, I. Dando, M.G. Romanelli, M. Kram-pera, G. Pizzolo, M. Donadelli, M.T. Scupoli. Effects of CD20 antibodies and kinase inhibitors on BCRsignalling and survival of CLL cells. British Journal of Haematology (2021), 192: 333-342

2. N. Piasentin, E. Milotti, R. Chignola. The control of acidity in tumor cells: a biophysical model.Scientific Reports (2020), 10: 13613

3. S. Patmanidis, R. Chignola, A. C. Charalampidis, G. P. Papavassilopolous. A comparison betweennonlinear least squares and maximum likelihood estimation for the prediction of tumor growth onexperimental data of human and rat origin. Biomedical Signal Processing and Control (2019), 54:101639

4. T. Fredrich, H. Rieger, R. Chignola, E. Milotti. Fine-grained simulations of the microenvironment ofvascularized tumours. Scientific Reports (2019), 9: 11698

5. L. Andolfi, S.L.M. Greco, D. Tierno, R. Chignola, M. Martinelli, E. Giolo, S. Luppi, I. Delfino, M.Zanetti, A. Battistella, G. Baldini, G. Ricci, M. Lazzarino. Planar AFM macro-probes to study thebiomechanical properties of large cells and 3D spheroids. Acta Biomaterialia (2019), 94: 505-513

6. G. Badino, R. Chignola. Fluctuations of atmospheric pressure and the sound of underground karstsystems: the Antro del Corchia case (Apuane Alps, Italy). Frontiers in Earth Science (2019), 7: 147

7. R. Chignola, M. Sega, B. Molesini, A. Baruzzi, S. Stella, E. Milotti. Collective radioresistance ofT47D breast carcnoma cells is mediated by a Syncytin-1 homologous protein. PLoS ONE (2019), 14:e0206713

8. S. Stella, R. Chignola, E. Milotti. Dynamical detection of boundaries and cavities in biophysical cell-based simulations of growing tumour tissues. IEEE/ACM Transactions on Computational Biology andBioinformatics (2019), 16: 1901-1911

9. C. Cavallini, R. Chignola, I. Dando, O. Perbellini, E. Mimiola, O. Lovato, C. Laudanna, G. Pizzolo,M. Donadelli, M.T. Scupoli. Low catalase expression confers redox hypersensitivity and identifies anindolent clinical behavior in CLL. Blood (2018), 131: 1942-1954

10. A. Menin, F. Zanoni, M. Vakarelova, R. Chignola, G. Donà, C. Rizzi, F. Mainente, G. Zoccatelli. Effectsof microencapsulation by ionic gelation on the oxidative stability of flaxseed oil. Food Chemistry(2018), 269: 293-299

11. B. Molesini, G.L. Rotino, V. Dusi, R. Chignola, T. Sala, G. Mennella, G. Francese, T. Pandolfini. Twometallocarboxypeptidase inhibitors are implicated in tomato fruit development and regulated by theInner No Outer transcription factor. Plant Science (2018), 266: 19-26

12. E. Milotti, V. Vyshemirsky, S. Stella, F. Dogo, R. Chignola. Analysis of the fluctuations of the tu-mor/host interface. Physica A (2017), 486: 587-594

13. G. Gonzato, G. Rossi, R. Chignola. Basalt intrusions in palaeokarst caves in the central LessiniMountains (Venetian Prealps, Italy). Acta Carsologica (2017), 46: 33-45

14. E. Milotti, S. Stella, R. Chignola. Pulsation-limited oxygen diffusion in the tumour microenvironment.Scientific Reports (2017), 7: 39762

15. D. Treggiari, G. Zoccatelli, R. Chignola, B. Molesini, P. Minuz, T. Pandolfini. Tomato cystine-knotminiproteins possessing anti-angiogenic activity exhibit in vitro gastrointestinal stability, intestinalabsorption and resistance to food industrial processing. Food Chemistry (2017), 221: 1346-1353


16. M. Vakarelova, F. Zanoni, P. Lardo, G. Rossin, F. Mainente, R Chignola, A. Menin, C. Rizzi, G. Zoc-catelli. Production of stable food-grade microencapsulated astaxanthin by vibrating nozzle technol-ogy. Food Chemistry (2017), 221: 289-295

17. S. P. Santero, F. Favretto, S. Zanzoni, R. Chignola, M. Assfalg, M. P. D’Onofrio. Effects of macro-molecular crowding on a small lipid binding protein probed at the single-aminoacid level. Archives ofBiochemistry and Biophysics (2016), 606: 99-110

18. F. Mainente, C. Rizzi, G. Zoccatelli, R. Chignola, B. Simonato, G. Pasini. Setup of a procedure forcider proteins recovery and quantification. European Food Research and Technology (2016), 242:1803-1811

19. V. Guglielmi, G. Vattemi, R. Chignola, A. Chiarini, M. Marini, I. Dal Pra, M. Di Chio, C. Chiamulera,U. Armato, G. Tomelleri. Evidence for caspase-dependent programmed cell death along with repairprocesses in affected skeletal muscle in patients with mitochondrial disorders. Clinical Science (2016),130: 167-181

20. A. Baruzzi, S. Remelli, E. Lorenzetto, M. Sega, R. Chignola, G. Berton. Sos1 regulates macrophagepodosome assembly and macrophage invasive capacity. The Journal of Immunology (2015), 195:4900-4912

21. C. Lombardo, M. Bolla, R. Chignola, G. Senna, G. Rossin, B. Caruso, C. Tomelleri, D. Cecconi,A. Brandolini, G. Zoccatelli. A study of the immunoreactivity of T. monococcum (Einkorn) wheatin patients with wheat-dependent exercise-induced anaphylaxis for he production of hypoallergenicfoods. Journal of Agricultural and Food Chemistry (2015), 63: 8299-8306

22. R. Chignola, M. Sega, S. Stella, V. Vyshemirsky, E. Milotti. From single-cell dynamics to scaling lawsin oncology. Biophysical Reviews and Letters (2014), 9: 273-284

23. M. Sega, R. Chignola. Population ecology of heterotypic tumour cell cultures. Cell Proliferation (2014),47: 476-483

24. G. Gonzato, A. Castellarin, R. Chignola, F. Gamberini, P. Lazzeri, Unione Speleologica Veronese. Newdating of paleokarst features at Torricelle hills (Verona, Italy). Italian Journal of Geosciences (2014),133: 427-438

25. S. Stella, R. Chignola, E. Milotti. Efficient and extendible class scheme for the combined reaction-diffusion of multiple molecular species. Computer Physics Communications (2014), 185: 826-835

26. E. Milotti, V. Vyshemirsky, M. Sega, S. Stella, R. Chignola. Metabolic scaling in solid tumours.Scientific Reports (2013), 3: 1938

27. E. Milotti, V. Vyshemirsky, M. Sega, S. Stella, F. Dogo, R. Chignola. Computer-aided biophysicalmodeling: a quantitative approach to complex biological systems. IEEE/ACM Transactions on Com-putational Biology and Bioinformatics (2013),10: 805-810

28. A. Cesano, O. Perbellini, E. Evensen, C.C. Chu, F. Cioffi, J. Ptacek, R.N. Damle, R. Chignola, J.Cordeiro, X. Yan, R.E. Hawtin, I. Nichele, J.R. Ware, C. Cavallini, O. Lovato, R. Zanotti, K.R. Rai, N.Chiorazzi, G. Pizzolo, M.T. Scupoli. Association between B-cell receptor responsiveness and diseaseprogression in B-cell chronic lymphocytic leukemia: results from single cell network profiling studies.Haematologica (2013), 98: 626-634

29. E. Milotti, V. Vyshemirsky, M. Sega, R. Chignola. Interplay between distribution of live cells andgrowth dynamics of solid tumours. Scientific Reports (2012), 2: 990

30. G. Zoccatelli, M. Sega, M. Bolla, D. Cecconi, P. Vaccino, C. Rizzi, R. Chignola, A. Brandolini. Expres-sion of α-amylase inhibitors in diploid Triticum species. Food Chemistry (2012), 135: 2643-2649

31. M. Sega, C. Zanetti, C. Rizzi, M. Olivieri, R. Chignola, G. Zoccatelli. Production and characterization ofmonoclonal antibodies for the quantification of potentially allergenic xylanases from Aspergillus niger.Food Additives and Contaminants. Part A. Chemistry, Analysis, Control, Exposure & Risk Assessment(2012), 29: 1356-1363


32. R. Chignola. La distribuzione di Poisson nel laboratorio biomedico. MatematicaMente (2012), 168

33. R. Chignola, E. Milotti. Bridging the gap between the micro– and the macro–world of tumors. AIPAdvances (2012), 2: 011204

34. M. Consolini, M. Sega, C. Zanetti, M. Fusi, R. Chignola, M. De Carli, C. Rizzi, G. Zoccatelli. Emulsifi-cation of simulated gastric fluids protects wheat alpha-amylase inhibitor 0.19 epitopes from digestion.Food Analytical Methods (2012), 5: 234-243

35. R. Chignola, M. Farina, A. Del Fabbro, E. Milotti. Modular model of TNFα cytotoxicity. Bioinformatics(2011), 27: 1754-1757

36. R. Chignola, A. Del Fabbro, M. Farina, E. Milotti. Computational challenges of tumor spheroidmodeling. Journal of Bioinformatics and Computational Biology (2011), 9: 559-577

37. R. Chignola, E. Milotti. Batteri, virus, mutazioni e statistica: l’esperimento di Luria e Delbrück.MatematicaMente (2011), 157

38. E. Milotti, R. Chignola. Emergent properties of tumor microenvironment in a real-life model of multi-cell tumor spheroids. PLoS ONE (2010), 5: e13942

39. R. Chignola, A. Del Fabbro, E. Milotti. Dynamics of intracellular Ca2+ oscillations in the presence ofmultisite Ca2+-binding proteins. Physica A - Statistical Mechanics and its Applications (2010), 389:3172-3178

40. A. Gliozzi, C. Guiot, R. Chignola, P. Delsanto. Oscillations in growth of multicellular tumor spheroids:a revisited quantitative analysis. Cell Proliferation (2010), 43: 344-353

41. C. Tomelleri, C. Dalla Pellegrina, R. Chignola. Microplate spectrophotometry for the high-throughputscreening of cytotoxic molecules. Cell Proliferation (2010), 43: 130-138

42. E. Milotti, A. Del Fabbro, R. Chignola. Numerical integration methods for large-scale biophysicalsimulations. Computer Physics Communications (2009), 180: 2166-2174

43. C. Dalla Pellegrina, O. Perbellini, M. T. Scupoli, C. Tomelleri, C. Zanetti, G. Zoccatelli, M. Fusi, A.Peruffo, C. Rizzi, R. Chignola. Effects of wheat germ agglutinin on human gastrointestinal epithelium:insights from an experimental model of immune/epithelial cells interaction. Toxicology and AppliedPharmacology (2009), 237: 146-153

44. E. Milotti, R. Chignola, C. Dalla Pellegrina, A. Del Fabbro, M. Farina, D. Liberati. VBL: VirtualBiophysics Lab. Il Nuovo Cimento (2008) 31C: 109-118

45. E. Milotti, A. Del Fabbro, C. Dalla Pellegrina, R. Chignola. Statistical approach to the analysis of celldesynchronization data. Physica A - Statistical Mechanics and its Applications (2008) 387: 4204-4214

46. C. Tomelleri, E. Milotti, C. Dalla Pellegrina, O. Perbellini, A. Del Fabbro, M. T. Scupoli, R. Chignola.A quantitative study on the growth variability of tumour cell clones in vitro. Cell Proliferation (2008)41: 177-191

47. G. Zoccatelli, C. Dalla Pellegrina, M. Consolini, M. Fusi, S. Sforza, G. Aquino, A. Dossena, R. Chig-nola, A. Peruffo, M. Olivieri, C. Rizzi. Isolation and identification of two lipid transfer proteins inpomegranate (Punica granatum). Journal of Agricultural and Food Chemistry (2007) 55: 11057-11962

48. R. Chignola, A. Del Fabbro, C. Dalla Pellegrina, E. Milotti. Ab initio phenomenological simulation ofthe growth of large tumor cell population. Physical Biology (2007) 4: 114-133

49. E. Milotti, A. Del Fabbro, C. Dalla Pellegrina, R. Chignola. Dynamics of allosteric action in multisiteprotein modification. Physica A - Statistical Mechanics and its Applications (2007), 379: 133-150

50. G. Zoccatelli, C. Dalla Pellegrina, S. Mosconi, M. Consolini, G. Veneri, R. Chignola, A. Peruffo, C.Rizzi. Full-fledged proteomic analysis of bioactive wheat amylase inhibitors by a three-dimensionalanalytical technique: Identification of new heterodimeric aggregation states. Electrophoresis (2007),28: 460-466


51. R. Chignola, C. Dalla Pellegrina, A. Del Fabbro, E. Milotti. Thresholds, long delays and stability fromgeneralized allosteric effect in protein networks. Physica A - Statistical Mechanics and its Applications(2006), 371: 463-472

52. R. Chignola, P. Dai Pra, L. M. Morato, P. Siri. Proliferation and death in a binary environment: astochastic model of cellular ecosystems. Bulletin of Mathematical Biology (2006), 68: 1661-1680

53. G. Veneri, G. Zoccatelli, S. Mosconi, C. Dalla Pellegrina, R. Chignola, C. Rizzi. A rapid method for therecovery, quantification and electrophoretic analysis of proteins from beer. Journal of the Institute ofBrewing (2006), 112: 26-27

54. R. Chignola, A. Del Fabbro, R. Foroni, E. Milotti. Modellizzazione biofisica per la crescita dei tumorisolidi. Automazione e Strumentazione (2005) 10: 87-93

55. C. Dalla Pellegrina, C. Rizzi, S. Mosconi, G. Zoccatelli, A. Peruffo, R. Chignola. Plant lectins as carriersfor oral drugs: is wheat germ agglutinin a suitable candidate? Toxicology and Applied Pharmacology(2005), 207: 170-178

56. C. Dalla Pellegrina, G. Padovani, F. Mainente, G. Zoccatelli, G. Bissoli, S. Mosconi, G. Veneri, A. Pe-ruffo, G. Andrighetto, C. Rizzi, R. Chignola. Anti-tumour potential of a gallic acid-containing phenolicfraction from Oenothera biennis. Cancer Letters (2005), 226: 17-25

57. R. Chignola, E. Milotti. A phenomenological approach to the simulation of metabolism and prolifera-tion dynamics of large tumor cell populations. Physical Biology (2005), 2: 8-22

58. S. Vincenzi, S. Mosconi, G. Zoccatelli, C. Dalla Pellegrina, G. Veneri, R. Chignola, A. Peruffo, A. Curi-oni, C. Rizzi. Protein recovery from wine and their quantification: development of a new procedure.American Journal of Enology and Viticulture (2005), 56: 182-187

59. R. Chignola, R. Foroni. Estimating the growth kinetics of experimental tumours from as few as twodeterminations of tumour size: implications for clinical oncology. IEEE Transactions on BiomedicalEngineering (2005), 52: 808-815

60. R. Chignola, E. Milotti. Numerical simulation of tumor spheroid dynamics. Physica A - StatisticalMechanics and its Applications (2004), 338: 261-266

61. C. Dalla Pellegrina, A. Matucci, G. Zoccatelli, C. Rizzi, S. Vincenzi, G. Veneri, G. Andrighetto, A.Peruffo, R. Chignola. Studies on the joint cytotoxicity of wheat germ agglutinin and monensin. Toxi-cology in Vitro (2004), 18: 821-827

62. A. Matucci, G. Veneri, C. Dalla Pellegrina, G. Zoccatelli, S. Vincenzi, R. Chignola, A. Peruffo, C.Rizzi. Temperature-dependent decay of Wheat Germ Agglutinin activity and its implications for foodprocessing and analysis. Food Control (2004), 15: 391-395

63. S. Sartoris, M. G. Testi, E. Stefani, R. Chignola, C. Guerriero, A. Matucci, T. Cestari, A. Scarpa, A.P. Riviera, G. Zanoni, G. Tridente, G. Andrighetto. The induction of an anti-tumor adaptive immuneresponse elicited by tumor cells expressing de novo B7-1 mainly depends on the anatomic site of theirdelivery. The dose applied regulates the expansion of the response. Immunology (2003), 11: 474-481

64. C. Rizzi, L. Galeoto, G. Zoccatelli, S. Vincenzi, R. Chignola, A. Peruffo. Active soybean lectin in foods:quantitative determination by ELISA using immobilised asialofetuin. Food Research International(2003), 36: 815-821

65. C. Guerriero, G. Zoccatelli, E. Stefani, S. Sartoris, T. Cestari, A. P. Riviera, G. Tridente, G. An-drighetto, R. Chignola. Myelin basic protein epitopes secreted by human T cells encounter naturalautoantibodies in the serum. Journal of Neuroimmunology (2003), 141: 83-89

66. C. Rizzi, R. Chignola, G. Zoccatelli, M. Donà, A. Peruffo, U. Carraro, K. Rossini. Effects of a WheatGerm-enriched diet on skeletal muscle regeneration in Whistar rats Italian Journal of Food Science(2003), 15: 417-425


67. G. Zoccatelli, C. Dalla Pellegrina, S. Vincenzi, C. Rizzi, R. Chignola, A. Peruffo. Egg-matrix for large-scale single-step affinity purification of plant lectins with different carbohydrate specificities. ProteinExpression and Purification (2003), 27: 182-185

68. S. Vincenzi, G. Zoccatelli, F. Perbellini, C. Rizzi, R. Chignola, A. Curioni, A. Peruffo. Quantitativedetermination of dietary lectin activities by enzyme-linked immunosorbent assay using specific gly-coproteins immobilized on microtiter plates. Journal of Agricultural and Food Chemistry (2002), 50:6266-6270

69. R. Chignola, C. Rizzi, S. Vincenzi, T. Cestari, N. Brutti, A. P. Riviera, S. Sartoris, A. Peruffo, G.Andrighetto. Effects of dietary wheat germ deprivation on the immune system in whistar rats: a pilotstudy. International Immunopharmacology (2002), 2:1495-1501

70. R. Chignola, G. Andrighetto, D. Liberati. Aggregati sperimentali di cellule tumorali: studio dellacrescita. Automazione e Strumentazione (2001), 9: 113-115

71. R. Chignola, T. Cestari, C. Guerriero, A. P. Riviera, S. Ferrari, A. Brendolan, M. Gobbo, S. Amato, S.Sartoris, G. Fracasso, M. G. Liuzzi, P. Riccio, G. Tridente, G. Andrighetto. Expression of myelin basicprotein (MBP)-like epitopes in human non-neural cells revealed by two anti-MBP IgM monoclonalantibodies. Clinical and Experimental Immunology (2000), 122: 429-436

72. S. Sartoris, A. Brendolan, A. Degola, M. G. Testi, R. Chignola, A. Scarpa, M. Scardoni, G. Contreras,L. Pinelli, C. Lunardi, R. Beri, C. Pera, G. B. Ferrara, A. P. Riviera, G. Tridente, G. Andrighetto. Anal-ysis of CIITA encoding AIR-1 gene promoters in insulin dependent diabetes mellitus and rheumatoidarthritis patients from the northeast of italy: absence of sequence variability. Human Immunology(2000), 61: 599-604

73. R. Chignola, A. Schenetti, G. Andrighetto, E. Chiesa, R. Foroni, S. Sartoris, G. Tridente, D. Liberati.Forecasting the growth of multicell tumour spheroids: implications for the dynamic growth of solidtumours. Cell Proliferation (2000), 33: 219-229

74. R. Chignola, D. Liberati, E. Chiesa, C. Anselmi, R. Foroni, S. Sartoris, A. Brendolan, G. Tridente, G.Andrighetto. A non-parametric method for the analysis of experimental tumour growth data. Medicaland Biological Engineering and Computing (1999), 37:537-542

75. R. Chignola, A. Schenetti, E. Chiesa, R. Foroni, S. Sartoris, A. Brendolan, G. Tridente, G. Andrighetto,D. Liberati. Oscillating growth patterns of multicellular tumour spheroids. Cell Proliferation (1999),32:39-48

76. R. Chignola, C. Anselmi, M. Dalla Serra, A. Franceschi, G. Fracasso, M. Pasti, E. Chiesa, M. J. Lord,G. Tridente, M. Colombatti. Self-potentiation of ligand-toxin conjugates containing ricin A chainfused with viral structures. Journal of Biological Chemistry (1995), 270:23345-23351

77. R. Chignola, R. Foroni, A. Franceschi, M. Pasti, C. Candiani, C. Anselmi, G. Fracasso, G. Tridente,M. Colombatti. Heterogeneous response of individual multicellular tumor spheroids to immunotoxinsand ricin toxin. British Journal of Cancer (1995), 72:607-614

78. R. Chignola, M. Pasti, C. Candiani, A. Franceschi, C. Anselmi, G. Tridente, M. Colombatti. Escapemechanisms of human leukemic cells to long-term immunotoxin treatment in an in vitro experimentalmodel. International Journal of Cancer (1995), 61:535-541

79. R. Chignola, R. Foroni, C. Candiani, A. Franceschi, M. Pasti, G. Stevanoni, C. Anselmi, G. Tridente,M. Colombatti. Cytoreductive effects of anti transferrin receptor immunotoxins in a multicellulartumor spheroid model. International Journal of Cancer (1994), 57:268-274

80. R. Chignola, C. Anselmi, A. Franceschi, M. Pasti, C. Candiani, G. Tridente, M. Colombatti. Sensitivityof human leukemia cells in exponential or stationary growth phase to anti-CD5 immunotoxins. Roleof intracellular processing events. Journal of Immunology (1994), 152:2333-2343

81. A. Franceschi, F. Dosio, C. Anselmi, R. Chignola, C. Candiani, M. Pasti, G. Tridente, M. Colombatti.Mechanisms involved in serum dependent inactivation of the immunotoxin enhancers monensin andcarrier protein-monensin. European Journal of Biochemistry (1994), 219:469-479


82. G. Benoni, C. Candiani, A. Franceschi, R. Chignola, M. Pasti, L. Cuzzolin, C. Anselmi, G. Stevanoni,G. Tridente, M. Colombatti. Disposition of intrathecally administered immunotoxins in rats. A pre-liminary report. Pharmacological Research (1992), 25:290-291

83. C. Candiani, A. Franceschi, R. Chignola, M. Pasti, C. Anselmi, G. Benoni, G. Tridente, M. Colombatti.Blocking effect of human serum but not of cerebrospinal fluid on ricin A chain immunotoxin potenti-ation by monensin or carrier protein-monensin conjugates. Cancer Research (1992), 52:623-630

84. C. Candiani, C. Anselmi, R. Chignola, A. Franceschi, M. Brentegani, G. Tridente, M. Colombatti.Potentiation of cytotoxic heteroconjugates by monensin-carrier proteins. Journal of Chemotherapy(1991), 3:351-353

85. M. Colombatti, R. Chignola, C. Candiani, A. Franceschi, C. Anselmi, G. Tridente. Immunotoxinssynthesized with ricin or diphtheria toxin: mechanisms of action and improvement of their anti-tumor cell efficacy. Journal of Chemotherapy (1991), 3:341-344

86. N. Franzolin, G. Bianchi, P. Beltrami, R. Chignola, R. Pianon, A. D’Amico, L. S. Azzolina, G. Tridente,G. Mobilio. Il ruolo dell’analisi del DNA mediante citometria a flusso nell’identificare i falsi positivicitologici da flogosi. Acta Urologica Italica (1991), 6:505-507

87. R. Chignola, M. Colombatti, L. Dell’Arciprete, C. Candiani, G. Tridente. Distribution of endocytosedmolecules to intracellular acidic environments correlates with immunotoxin activity. InternationalJournal of Cancer (1990), 46:1117-1123

88. M. Colombatti, L. Dell’Arciprete, R. Chignola, G. Tridente. Carrier protein-monensin conjugates:

enhancement of immunotoxin cytotoxicity and potential in tumor treatment. Cancer Research (1990),

50:1385-1391


Refereed papers in books and in proceedings

1. E. Milotti, T. Fredrich, R. Chignola, H. Rieger. Oxygen in the tumor microenvironment: mathematicaland numerical modeling. In:Tumor Microenvironment, Advances in Experimental Medicine and Biologybook series (AEMB, volume 1259), A. Birbair Ed., Springer Nature, Switzerland, AG 2020, pp. 53-75,ISBN 978-3-030-43092-4

2. R. Chignola, M. Sega, S. Stella, V. Vyshemirsky, E. Milotti. From single-cell dynamics to scaling lawsin oncology. In: Research on the physics of cancer: a global perspective, Bernard S. Gerstman, Ed.,World Scientific Publishing, Singapore (2016), pp. 97-108, ISBN 978-981-4730-25-9

3. S. Stella, F. Dogo, E. Milotti, R. Chignola. Neighbor search algorithm for lattice-free simulations withshort-range forces. High Performance Computing and Simulations (HPCS), Bologna (2014) pp.621-626

4. S. Stella, R. Chignola, E.Milotti. Use of GPUs to boost the performance of a lattice-free tumour growthmodel. Published in: Journal of Physics: Conference Series (2014) 566: 012019

5. S. Stella, R. Chignola, F. Dogo, M. Sega, E. Milotti. 3D modeling of tumor growth. Cross-border Italy-Slovenia Biomedical Research, Trieste. In: S. Passamonti, S. Gustincich, T. Lah Turnšek, B. Peterlin,R. Pišot, P. Storici (Eds.), e-ISBN 978-88-8303-573-9 (2014), pp. 195-199

6. S Stella, R. Chignola, E. Milotti. Competing computational approaches to reaction-diffusion equationsin clusters of cells. International Conference on Mathematical Modeling in Physical Sciences, IC-MSQUARE, Prague, Czech Republic, Sep. 1-5 2013. Published in: Journal of Physics: ConferenceSeries (2014), 490: 012129

7. E. Milotti, R. Chignola. Physical and computational issues in a simulation of multicellular tumorspheroids. Proceedings of the 4th International Advanced Research Workshop on In Silico Oncol-ogy and Cancer Investigation, Athens, Greece, Sept. 35-38 (2010), pp.1-3

8. R. Chignola, E. Milotti. Tumor microenvironment in a real-life model of tumor spheroids. Proceedings ofthe 4th International Advanced Research Workshop on In Silico Oncology and Cancer Investigation,Athens, Greece, Sept. 8-9 2010, pp.32-34

9. E. Milotti, A. Del Fabbro, M. Farina, D. Liberati, R. Chignola. Precision and stability issues in VBL, theVirtual Biophysics Lab simulation program. Proceedings of the meeting, ECOBIOSYS 2009: Calssifi-cation and forecasting models, May 15, 2009, Milano, pp. 1-6

10. R. Chignola. Studio della crescita di popolazioni biologiche interagenti. In: A.Burato, R.Chignola,L.Corso, Simulazione del comportamento dinamico di sistemi preda-predatore, I quaderni del Marconi- Quaderni didattici, Verona, Gente del Marconi, 2009, pp. 9-24

11. R. Chignola. Matematica e radioterapia dei tumori. In: A. Burato, R. Chignola, F. Castelli, L. Corso,G. Pezzo, S. Zuccher, Matematica e radioterapia dei tumori: sviluppo e applicazioni di un modellopredittivo semplificato, I quaderni del Marconi - Quaderni didattici, Verona, Gente del Marconi, 2007,pp. 9-21

12. R. Chignola. Il problema biologico. In: R. Chignola, F. Castelli, L. Corso, G. Pezzo, S. Zuccher Labiomatematica in un problema di oncologia, I quaderni del Marconi - Quaderni didattici, Verona,Gente del Marconi, 2006, pp. 8-25

13. R. Chignola, A. Del Fabbro, R. Foroni, E. Milotti. Crescita dei tumori solidi: un approccio multi-disciplinare che attraversa la fenomenologia e la modellizzazione biofisica per approdare alla clinica.Proceedings of BIOSYS 2005, Sistemi di Ingegneria Biomedica, Milano, 9-10 Giugno 2005, pp. 1-16

14. E. Stefani, M. G. Testi, A. Matucci, R. Chignola, T. Cestari, S. Dalla Santa, A. Zoso, A. Rosato, G.Tridente, G. Andrighetto, S. Sartoris. Induction and expansion of an anti-tumor immune response byB7-1+ tumor cells in dependence on the anatomical site of delivery and the dose applied. Proceedingsof Immunology 2004, Montreal, Canada (2004), pp.521-524


15. R. Chignola, G. Andrighetto, D. Liberati. Analysis of biomedical signals: growth of experimental tumourcell aggregates. In: Health and Medical Systems: Information and Control Technologies, D.Liberatied., (2001) pp. 141-148

16. R. Chignola, D. Liberati, E. Chiesa, R. Foroni, G. Andrighetto, G. Tridente, M. Colombatti. On thegrowth dynamics of multicellular tumor spheroids: a preliminar report. In: Chaos Fractals Models, F.M.Guindani and G. Salvadori eds., Italian University Press, Pavia (1998), pp. 371-377

17. M. Colombatti, R. Chignola, C. Candiani, C. Anselmi, G. Tridente. Problems and potentials in immuno-toxin therapy. In: Immunological aspects of malignant lymphomas and cryoglobulinemia, F.Dammacco

ed., Edi-Ermes, Milano (1990) pp.147-159


Preprints

1. N. Piasentin, E. Milotti, R. Chignola. The control of acidity in tumor cells; a biophysical model, 2020(bioRxiv, DOI: 2020.03.22.002113)

2. T. Fredrich, H. Rieger, R. Chignola, E. Milotti. Fine-grained simulations of the microenvironment ofvascularized tumours., 2019(arXiv:1906.02441) and (bioRxiv: 661603)

3. R. Chignola, M. Sega, B. Molesini, A. Baruzzi, S. Stella, E. Milotti. Collective radioresistance of T47Dbreast carcnoma cells is mediated by a Syncytin-1 homologous protein, 2018(bioRxiv: 448217)

4. R. Chignola, A. Del Fabbro, M. Farina, E. Milotti. Computational challenges of tumor spheroidmodeling, 2010(arXiv:1012.2125)

5. E. Milotti and R Chignola. Emergent Properties of Tumor Microenvironment in a Real-life Model ofMulticell Tumor Spheroids, 2010(arXiv:1010.1965)

6. R. Chignola, M. Farina, A. Del Fabbro, E. Milotti. Ab initio computational modeling of tumorspheroids, 2009(arXiv:0911.0596)

7. R. Chignola, A. Del Fabbro, E. Milotti. Dynamics of intracellular Ca2+ oscillations in the presence ofmultisite Ca2+-binding proteins, 2009(arXiv:09091918)

8. R. Chignola, M. Farina, A. Del Fabbro, E. Milotti. Balance between cell survival and death: a minimalquantitative model of tumor necrosis factor alpha cytotoxicity, 2009(arXiv:0905.4396)

9. E. Milotti, A. Del Fabbro, R. Chignola. Precision and Stability Issues in VBL, the Virtual BiophysicsLab simulation program, 2009(arXiv:0903.5089)

10. E. Milotti, A. Del Fabbro, R. Chignola. Numerical integration methods for large-scale biophysicalsimulations, 2009(arXiv:0903.5036)

11. C. Tomelleri, E. Milotti, C. Dalla Pellegrina, O. Perbellini, A. Del Fabbro, M.T. Scupoli, R. Chignola. Aquantitative study on the growth and variability of tumor cell clones in vitro, 2007(arXiv:0704.2132)

12. R. Chignola, C. Dalla Pellegrina, A. Del Fabbro, E. Milotti. Ab initio phenomenological simulation ofthe growth of large tumor cell populations, 2007(arXiv:physics/0703007)

13. E. Milotti, A. Del Fabbro, C. Dalla Pellegrina, R. Chignola. Dynamical hysteresis in multisite proteinmodification, 2007(arXiv:physics/0702094)

14. E. Milotti, A. Del Fabbro, C. Dalla Pellegrina, R. Chignola. Dynamics of allosteric action in multisiteprotein modification, 2006(arXiv:physics/0609227)

15. A. Del Fabbro, R. Chignola, E. Milotti. Fine-grained Delaunay triangulation in a simulation of tumorspheroid growth, 2006(arXiv:physics/0603001)


16. R. Chignola, C. Dalla Pellegrina, A. Del Fabbro, E. Milotti. Thresholds, long delays and stability fromgeneralized allosteric effect in protein networks, 2006(arXiv:q-bio/0601045)

17. R. Chignola, E. Milotti. A phenomenological approach to the simulation of metabolism and prolifera-

tion dynamics of large tumor cell populations, 2005

(arXiv:physics/0510110)


Abstracts and talks: International Meetings

1. E. Binatti, R. Chignola. Targeting macrophages with astaxanthin-loaded microparticles: a potentialway to attenuate radiation-induced fibrosis, SfRBM 27th annual conference, Free Radical Biology andmedicine (2020), 159, Supplement 1, S39

2. T. Fredrich, S. Stella, H. Rieger, R. Chignola, E. Milotti. Progress in cell-based, quasi-lattice-freesimulations of vascularized tumours, Virtual Physiological Human 2018, Conference, VPH for in silicoMedicine, Zaragoza, Spain (2018)

3. R. Chignola, M. Sega, B. Molesini, A. Baruzzi, S. Stella, E. Milotti. Survival probability of human breastcarcinoma cells to radiation treatment: role of cell fusion and of a Syncytin-1 homologous protein, 25thBiennal Congress of the European Association for Cancer Research, Amsterdam, The Netherlands(2018)

4. S. Stella, R. Chignola, E. Milotti. Insights into cancer progression from in-silico simulations of solid tu-mors with the (Virtual Biophysics Laboratory) VBL program, 8th Symposium on the Physics of Cancer,Leipzig, Germany (2017)

5. C. Cavallini, O. Perbellini, R. Chignola, M. Cordani, E. Zoratti, E. Mimiola, C. Laudanna, M. Don-adelli, G. Pizzolo, M.T. Scupoli. Redox signaling hypersensitivity distinguishes chronic lymphocyticleukemia patients with favorable prognosis, 21st Congress of the European Hematology Association,Copenhagen, Denmark (2016)

6. E. Milotti, R. Chignola, S. Stella. Tackling the complexity of multiscale, lattice-free models of tumorgrowth, Challenges in data science: a complex systems perspective, Turin, Italy (2015)

7. E. Milotti, S. Stella, F. Dogo, R. Chignola. Computational and mathematical approaches to tumormetabolism and microenvironment, 2nd ISCaM meeting - Metabolism and Microenvironment in CancerPlasticity - Venice, Italy (2015)

8. S. Zanzoni, M. Sega, M. D’Onofrio, M. Assfalg, R. Chignola, H. Molinari. Delivery of isotope-labeledproteins into eukaryotic cells for in-cell NMR experiments, Joint conference of the German, Italian andSlovenian Magnetic Resonance Societies, Frauenchiemsee, Germany (2013)

9. E. Milotti, R. Chignola. Physical and computational issues in a simulation of multicellular tumorspheroids, 4th International Advanced Research Workshop on In Silico Oncology and Cancer In-vestigation, Athens, Greece (2010)

10. R. Chignola, E. Milotti. Tumor microenvironment in a real-life model of tumor spheroids, 4th Interna-tional Advanced Research Workshop on In Silico Oncology and Cancer Investigation, Athens, Greece(2010)

11. O. Perbellini, F. Cioffi, R. Chignola, R. Zanotti, F. Aprili, A. Barbieri, O. Lovato, G. Pizzolo, M. T.Scupoli. Single-cell profiles of B-cell receptor phospho-protein networks associated with prognosis andprogression in chronic lymphocytic leukemia, 15th Congress of Hematology Association, Barcelona,Spain. Hematologica/The Hematology Journal (2010) 95 (Suppl. 2): 5.42

12. M. T. Scupoli, O. Perbellini, F. Cioffi, R. Chignola, E. Evensen, R. Zanotti, I. Nichele, O. Lovato, G.Pizzolo. BCR-signaling profiles associated with prognosis and progression in B-CLL, 15th Congress ofHematology Association, Barcelona, Spain. Hematologica/The Hematology Journal (2010) 95 (Suppl.2)

13. M. T. Scupoli, O. Perbellini, F. Cioffi, R. Chignola, E. Evensen, R. Zanotti, I. Nichele, O. Lovato,G. Pizzolo. BCR-signaling profiles associated with prognosis and progression in B-CLL, EducationalCancer Convention, What is new in Hemato-Oncology? Lugano, Switzerland (2010)

14. O. Perbellini, F. Cioffi, R. Chignola, R. Zanotti, F. Aprili, A. Barbieri, O. Lovato, G. Pizzolo, M. T.Scupoli. Single-cell profiles of B-CLL receptor phospho-protein networks associated with prognosis andprogression in chronic lymphocytic leukemia, XIII International Workshop on Chronic LymphocyticLeukemia, Barcelona, Spain (2009)


15. R. Chignola. Ab initio computational modeling of tumor spheroids, 1st Transatlantic Workshop onMulti-Scale Cancer Modeling, ICT-BIO2008, Brussels (2008)

16. G. Zoccatelli, M. Consolini, M. Fusi, C. Dalla Pellegrina, R. Chignola, A. Peruffo, F. Marsano, M.San Miguel-Moncin, S. Scheurer, C. Rizzi. Pomegranate LTP isoforms identified by a new proteomicapproach show different immunological properties. Allergy (2008) 63 (Suppl. 88): 119

17. R. Chignola, C. Dalla Pellegrina, A. Del Fabbro, E. Milotti. Numerical simulation of metabolism, growthand proliferation of tumor cells, Conference on Computational Physics (CCP2007), Bruxelles (2007)

18. R. Chignola. Metabolism and cell population dynamics, Gordon Research Conference Metabolism inEcology, Bates College, Portland (ME), USA (2006)

19. C. Dalla Pellegrina, A. Peruffo, R. Chignola. At nanomolar concentrations Wheat Germ Agglutinin mightaffect the immune response in vitro, 10th Workshop on the developments in the Italian PhD researchin Food Science and Technology, Foggia, Italy (2005)

20. S. Mosconi, C. Dalla Pellegrina, C. Rizzi, R. Chignola. Cytotoxic effects on Caco-2 cells of a wheatprotein fraction containing alpha-amylase inhibitors, EFFoST 2005 INTRAFOOD Conference, Valencia,Spain (2005)

21. C. Dalla Pellegrina, E. Milotti, A. Del Fabbro, R. Chignola. Effects of the continuous and intermittentglucose deprivation on the growth and survival of human leukemic cells, ELSO Meeting, Dresden,Germany (2005)

22. R. Chignola, E. Milotti. Fine-grained Delaunay triangulation for the solution of diffusion equations incellular clusters, Complexity in the Living, Roma, Italy (2004)

23. R. Chignola, E. Milotti. Numerical simulation of tumor spheroid dynamics, International Conferenceon Frontier Science, Pavia, Italy (2003)

24. R. Chignola. Mathematical modelling of multicellular tumour spheroid growth: implications for thegrowth of solid tumours, Advances in the use of multicellular spheroids in cancer biology and therapy,Roma, Italy (2002)

25. R. Chignola, D. Liberati, E. Chiesa, R. Foroni, S. Sartoris, A. Brendolan, G. Tridente, G. Andrighetto.Non-parametric analysis of spheroid growth data, Sixth International Conference of Anticancer Re-search, Kallithea, Greece. Anticancer Res. (1998) 18(6C): 4844

26. R. Chignola, E. Chiesa, R. Foroni, S. Sartoris, A. Brendolan, G. Tridente, G. Andrighetto, D. Liberati.Growth dynamics of multicell tumour spheroids, Sixth International Conference of Anticancer Re-search, Kallithea, Greece. Anticancer Res. (1998) 18(6C): 4844

27. R. Chignola, G. Andrighetto, R. Foroni, M. Colombatti. Growth dynamics of multicellular tumorspheroids, International Conference Chaos, Fractals and Models, Pavia (1996)

28. R. Chignola, M. Colombatti. Virus-toxin chimaeras for the generation of more potent immunotoxins,Drug Carriers in Biology and Medicine, Gordon Research Conferences, Ventura, CA, USA (1996)

29. R. Chignola, M. Pasti, A. Franceschi, C. Candiani, C. Anselmi, G. Tridente, M. Colombatti. Cytore-ductive effects of immunotoxins in an in vitro micrometastases model, Drug Carriers in Biology andMedicine, Gordon Research Conferences, Tilton, NH, USA (1994)

30. R. Chignola, F. Gerosa, R. Foroni, G. Stevanoni, M. Tommasi, A. Franceschi, C. Candiani, M. Pasti,G. Tridente, M. Colombatti. Sensitivity of monolayer and spheroid cultures to immunotoxins and LAKcells, 84th Annual Meeting, American Association for Cancer Research, Orlando, USA (1993)

31. R. Chignola, C. Anselmi, A. Franceschi, M. Pasti, C. Candiani, G. Tridente, M. Colombatti. Cell growthkinetics and sensitivity to immunotoxin treatment, XIIIth Washington International Spring Symposium,Washington D.C., USA (1993)


32. C. Candiani, A. Franceschi, R. Chignola, M. Pasti, G. Benoni, G. Stevanoni, C. Anselmi, L. Cuzzolin,G. Tridente, M. Colombatti. Enhancement of RTA-IT by HSA-monensin. Effect of human serum or CSFand potential applications in brain tumor therapy, Third International Symposium on Immunotoxins,Orlando, USA (1992)

33. R. Chignola, C. Anselmi, G. Stevanoni, C. Candiani, A. Franceschi, M. Pasti, R. Foroni, G. Tridente, M.Colombatti. Immunotoxin efficacy is influenced by cell growth kinetics, Third International Symposiumon Immunotoxins, Orlando, USA (1992)

34. L. Dell’Arciprete, M. Colombatti, G. Stevanoni, E. Chiesa, R. Chignola, G. Tridente. Potentiation ofantibody-toxin or ligand-toxin cytotoxicity by HSA-Monensin, 7th International Congress of Immunol-ogy, Berlin, Germany (1989)

35. R. Chignola, M. Colombatti, L. Dell’Arciprete, G. Stevanoni, G. Tridente. Intracellular fate of cell-surface bound molecules investigated by flow cytometry, 7th International Congress of Immunology,

Berlin, Germany (1989)


Abstracts and talks: National Meetings

1. C. Cavallini, R. Chignola, I. Dando, O. Perbellini, E. Mimiola, O. Lovato, M. Donadelli, M.T. Scupoli.Phospho-specific flow cytometry for characterizing redox signaling sensitivity associated with leukemiadisease progression, 2nd Meeting of the Italian Society for Clinical Cell Analysis, Bologna (2017)

2. C. Cavalini, O. Perbellini, R. Chignola, I. Dando, E. Zoratti, E. Mimiola, M. Donadelli, C. Laudanna,G. Pizzolo, M.T. Scupoli. Redox signaling hypersensitivity is associated with favorable prognosis inchronic lymphocytic leukemia patients, 46◦ Congresso Nazionale della Società Italiana di Ematologia,Roma (2017)

3. D. Treggiari, G. Zoccatelli, R Chignola, B. Molesini, P. Minuz, T. Pandolfini. Bioavailability of tomatocysteine-knot miniproteins with anti-angiogenic properties, XIV FISV Congress, Roma (2016)

4. S. Stella, R. Chignola, E. Milotti. Accelerating biophysics simulations with Graphics Processing Units,102◦ Congresso Nazionale della Società Italiana di Fisica, Padova (2016)

5. R. Chignola, P. Donato, R. Massa, E. Milotti. Microwave spectroscopy for biomarkers detection, VCongresso del Gruppo Nazionale di Bioingegneria, Napoli (2016)

6. R. Chignola, M. Sega, A. Baruzzi, S. Stella, E. Milotti. Resistance of T47D human breast carcinomacells to ionizing radiations, 58th Annual Meeting of the Italian Cancer Society, Verona (2016)

7. C. Cavallini, O. Perbellini, R. Chignola, I. Dando, E. Zoratti, E. Mimiola, M. Donadelli, C. Laudanna,G. Pizzolo, M.T. Scupoli. Association of reactive oxygen species-mediated signal transduction withfavourable prognosis in chronic lymphocytic leukemia patients, 58th Annual Meeting of the ItalianCancer Society, Verona (2016)

8. E. Milotti, S. Stella, R. Chignola. Synthetic life in extreme conditions, 5th Workshop of the ItalianAstrobiology Society, Trieste (2015)

9. M. Sega, R. Chignola. Radioresistance of breast cancer T47D cells grown as 3D cultures, 27th AnnualConference of Italian Association of Cell Cultures (ONLUS-AICC), Verona (2014)

10. C. Giulia, M. Sega, A. Carcereri de Prati, E. Butturini, R. Chignola, S. Mariotto. Chemosensitization ofbreast cancer cells to chemotherapy through HIF-1alpha inhibition in hypoxic environment: preliminarystudies, 27th Annual Conference of Italian Association of Cell Cultures (ONLUS-AICC), Verona (2014)

11. R. Chignola, V. Vyshemirsky, S. Stella, M. Sega, F. Dogo, E. Milotti. From tumor microenvironment dy-namics to scaling-laws in oncology, XCIX Congresso Nazionale della Società Italiana di Fisica, Trieste(2013)

12. S. Zanzoni, M. Sega, M. D’Onofrio, M. Assfalg, R. Chignola, H. Molinari. High resolution nuclearmagnetic resonance methods to monitor molecular probes in wukaryotic cells, XLI Congresso Nazionalesulla Risonanza Magnetica, Pisa (2012)

13. R. Chignola. Problemi di scala e scambio di informazioni, Oncogenesi: tra scienza e clinica medica,Frascati, Roma (2010)

14. M. T. Scupoli, O. Perbellini, F. Cioffi, R. Chignola, R. Zanotti, F. Aprili, A. Barbieri, O. Lovato, G.Pizzolo. Single-cell profiles of B-CLL receptor phospho-protein networks are associated with prognosisand progression in chronic lymphocytic leukemia, 42◦ Congresso della Società Italiana di Ematologia,Milano. Haematologica/the hematology journal (2009) 94: suppl. 4

15. O. Perbellini, F. Cioffi, R. Chignola, R. Zanotti, F. Aprili, A. Barbieri, O. Lovato, G. Pizzolo, M. T.Scupoli. Flow cytometry analysis of B-cell receptor phospho-protein networks in chronic lymphocyticleukemia, XXVII Conferenza Nazionale della Società Italiana di Citometria, Ferrara (2009)

16. R. Chignola, A. Del Fabbro, R. Foroni, E. Milotti. Crescita dei tumori solidi: un approccio multi-disciplinare che attraversa la fenomenologia e la modellizzazione biofisica per approdare alla clinica,BIOSYS 2005, Sistemi di Ingegneria Biomedica, Milano, (2005)


17. R. Chignola, A. Del Fabbro, E. Milotti. Modello viscoelastico di cellule animali, 90◦ Congresso Nazionaledella Società Italiana di Fisica, Brescia (2004)

18. R. Chignola. Aspetti e problematiche di tossicologia deli OGM, Alimenti e OGM: quali problematiche?,Associazione Italiana di Tecnologia Alimentare, Verona (2002)

19. R. Chignola, E. Milotti. Simulazioni numeriche della crescita di sferoidi tumorali, LXXXVIII CongressoNazionale della Società Italiana di Fisica, Alghero (2002)

20. R. Chignola. Ordine e disordine in sistemi naturali, Neuromat-II, Matematica e Neuroscienze, Pavia(2001)

21. R. Chignola, G. Andrighetto, D. Liberati. Analysis of biomedical signals: growth of experimental tumourcell aggregates, Sanità e Sistemi Medicali: Automazione ed Informatizzazione, Associazione NazionaleItaliana per l’Automazione, Genova (2001)

22. R. Chignola, C. Guerriero, T. Cestari, S. Bresciani, A. P. Riviera, S. Sartoris, M. G. Testi, G. Tridente,G. Andrighetto. Identification and possible role of MBP-epitopes expressed outside the nervous system,XI Congresso Nazionale della Associazione Italiana di Neuroimmunologia, Genova (2000)

23. R. Chignola, D. Liberati. Un modello di crescita degli sferoidi multicellulari, Metodi e Modelli dellaFisica Matematica nella Ricerca contro i Tumori, Torino (2000)

24. R. Chignola, T. Cestari, A. Brendolan, G. Fracasso, C. Guerriero. M. Gobbo, S. Amato, A. P. Riviera,S. Sartoris, P. Riccio, G. Tridente, G. Andrighetto. Expression of myelin basic protein (MBP)-like epi-topes in human non-neural cells revealed by two novel anti-MBP monoclonal antibodies, X CongressoNazionale della Associazione Italiana di Neuroimmunologia, Firenze (1999)

25. R. Chignola, D. Liberati, G. Andrighetto, G. Tridente, M. Colombatti. Dinamiche di crescita di micro-masse tumorali (sferoidi), Workshop Interdisciplinare Modelli Matematici in Biologia, Università degliStudi di Modena, Accademia di Scienze Lettere ed Arti di Modena, Modena (1997)

26. R. Chignola, T. Cestari, A. Brendolan, M. Colombatti, G. Tridente, G. Andrighetto. Analisi quantitativadell’interazione tra stimolo antigenico ed IL-2 in linfociti umani del sangue periferico, Workshop Inter-disciplinare Modelli Matematici in Biologia, Università degli Studi di Modena, Accademia di ScienzeLettere ed Arti di Modena, Modena (1997)

27. R. Chignola, C. Candiani, A. Franceschi, M. Pasti, C. Anselmi, E. Chiesa, G. Tridente, M. Colombatti.Cytotoxic efficacy of immunotoxins against solid tumors evaluated in an experimental micrometastasesmodel, Università ed Innovazione Biotecnologica, Consorzio Interuniversitario per le Biotecnologie,Brescia (1994)

28. R. Chignola, A. Franceschi, C. Candiani, M. Pasti, C. Anselmi, E. Chiesa, G. Tridente, M. Colombatti.Cytofluorimetric evaluation of immunotoxin internalization, Eur. J. Histochem. (1993) 37:70, suppl

29. C. Candiani, M. Donini, G. Stevanoni, A. Franceschi, M. Pasti, R. Chignola, C. Anselmi, G. Benoni,G. Tridente, M. Colombatti. Biodisponibilità e farmacocinetica di immunotossine nel SNC di ratto, 21◦

Congresso Nazionale del Gruppo di Cooperazione in Immunologia, Viterbo (1993)

30. M. Pasti, A. Franceschi, R. Chignola, C. Candiani, C. Anselmi, E. Chiesa, G. Tridente, M. Colom-batti. Identificazione degli epitopi T immunodominanti della catena A della ricina (RTA), 21◦ ConvegnoNazionale del Gruppo di Cooperazione in Immunologia, Viterbo (1993)

31. R. Chignola, R. Foroni, A. Franceschi, M. Pasti, C. Candiani, G. Stevanoni, C. Anselmi, G. Tridente, M.Colombatti. Efficacia citotossica di immunotossine in un modello tridimensionale di crescita tumorale,21◦ Convegno Nazionale del Gruppo di Cooperazione in Immunologia, Viterbo (1993)

32. G. Stevanoni, C. Candiani, A. Franceschi, R. Chignola, L. Cuzzolin, M. Pasti, C. Anselmi, G. Benoni,G. Tridente, M. Colombatti. Biodisponibilità di immunotossine intratecali, 20◦ Congresso Nazionaledel Gruppo di Cooperazione in Immunologia, Perugia (1992)


33. R. Chignola, C. Anselmi, M. Pasti, C. Candiani, A. Franceschi, G. Stevanoni, G. Tridente, M. Colom-batti. Differente effetto citotossico di immunotossine in cellule tumorali quiescenti o in attiva prolifer-azione, 20◦ Congresso Nazionale del Gruppo di Cooperazione in Immunologia, Perugia (1992)

34. A. Franceschi, C. Anselmi, C. Candiani, R. Chignola, M. Pasti, G. Stevanoni, G. Tridente, M. Colom-batti. Fenomeni coinvolti nell’inibizione di monensina come potenziante di immunotossine da parte disiero umano, 20◦ Congresso Nazionale del Gruppo di Cooperazione in Immunologia, Perugia (1992)

35. C. Candiani, A. Franceschi, R. Chignola, M. Pasti, C. Anselmi, G. Stevanoni, G. Benoni, G. Tridente,M. Colombatti. Potenziamento di immunotossine mediante monensina e suoi derivati. Effetti di siero eliquor umani, 7◦ Congresso della Associazione Italiana di Immunofarmacologia, Stresa (1991)

36. A. Franceschi, C. Candiani, F. Dosio, R. Chignola, M. Pasti, C. Anselmi, G. Stevanoni, G. Tridente,M. Colombatti. Il siero umano inibisce il potenziamento di immunotossine da parte dello ionoforo mo-nensina. Studio dei meccanismi e dei fattori coinvolti, 7◦ Congresso della Associazione Italiana diImmunofarmacologia, Stresa (1991)

37. C. Candiani, A. Franceschi, R. Chignola, M. Pasti, G. Benoni, C. Anselmi, G. Stevanoni, G. Tridente,M. Colombatti. Antibody/ligand-Toxin hybrid molecules for brain tumor immunotherapy, Journal ofNeuroimmunology (1991), 1:96 suppl.

38. C. Candiani, A. Franceschi, R. Chignola, M. Pasti, G. Benoni, C. Anselmi, G. Stevanoni, M. Gerosa,G. Tridente, M. Colombatti. Immunotoxins in brain tumor immunotherapy, The Italian Journal ofNeurological Sciences (1991), 5: 492

39. G. Bianchi, P. Beltrami, N. Franzolin, R. Chignola, P. Curti, D. Schiavone, C. Tallarigo, G. Malossini,L. S. Azzolina, G. Mobilio. L’analisi del DNA mediante citometria a flusso nei tumori del rene, ActaUrologica Italica (1991), 2: 82

40. F. Ricci, V. Tubini, G. Lazzara, L. Azzolina, R. Chignola, G. Zamboni, E. Laterza, G. Inaspettato.Correlazione tra citoflussimetria e istologia nell’Esofago di Barret, III Congresso Nazionale della SocietàPolispecialistica Italiana dei Giovani Chirurghi, Bari (1990)

41. C. Candiani, M. Colombatti, C. Anselmi, R. Chignola, A. Franceschi, M. Brentagani, G. Tridente.Potenziamento di eteroconiugati citotossici mediante composti monensina-proteina carrier, 6◦ Congressodella Associazione Italiana di Immunofarmacologia, Firenze (1990)

42. N. Franzolin, G. Bianchi, R. Pianon, P. Beltrami, A. D’Amico, R. Chignola, A. Iannucci, G. Mobilio.Il ruolo dell’analisi del DNA mediante citometria a flusso nell’identificare i falsi positivi citologici daflogosi, Acta Urologica Italica (1990), 2: 115

43. G. Bianchi, P. Beltrami, R. Chignola, N. Franzolin, C. Tallarigo, A. D’Amico, D. Schiavone, A. Ian-nucci, G. Mobilio. La citometria a flusso su agoaspirato e su campioni di tessuto di tumori renali, ActaUrologica Italica (1990), 2: 81

44. C. Candiani, M. Colombatti, C. Anselmi, R. Chignola, A. Franceschi, M. Brentegani, G. Tridente.Analisi della stabilità di coniugati HSA-Mo come potenzianti di eteroconiugati citotossici, XI CongressoNazionale della Società Italiana di Immunologia e Immunopatologia, Verona (1990)

45. R. Chignola, C. Anselmi, M. Colombatti, A. Franceschi, C. Candiani, M. Brentegani, G. Tridente.Produzione di tossine ricombinanti chimeriche per una aumentata citotossicità di immunotossine, XICongresso Nazionale della Società Italiana di Immunologia e Immunopatologia, Verona (1990)

46. R. Chignola, M. Colombatti, C. Candiani, A. Franceschi, C. Anselmi, M. Brentegani, G. Tridente.Parametri citofluorimetrici correlano con l’attività di eteroconiugati citotossici, XI Congresso Nazionaledella Società Italiana di Immunologia e Immunopatologia, Verona (1990)

47. R. Chignola, M. Colombatti, L. Dell’Arciprete, E. Chiesa, G. Stevanoni, G. Tridente. L’analisi citofluo-rimetrica dei processi di endocitosi può guidare nella selezione di eteroconiugati citotossici più efficaci,17◦ Convegno Nazionale del Gruppo di Cooperazione in Immunologia, Cortona (1989)


48. R. Chignola, M. Colombatti, L. Dell’Arciprete, G. Stevanoni, E. Chiesa, G. Tridente. Le vie di proces-sazione intracellulari possono essere seguite con precisione al FACS, Convegno del Gruppo di Cooper-

azione in Immunologia, Madonna di Campiglio (1989)

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