RNAi pathway components and function in Paramecium bursaria · 2021. 5. 20. · Paramecium...
Transcript of RNAi pathway components and function in Paramecium bursaria · 2021. 5. 20. · Paramecium...
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RNAi pathway components and function inParamecium bursaria
Finlay Maguire
University of Exeter
June 2, 2016
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Overview
Motivation
RNAi in ciliates
Experimental RNAi induction in P. bursaria
RNAi pathway components in active P. bursaria transcriptome(s)
In-silico analysis of potential endosymbiont ‘cross-talk’
Conclusions
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Why is Paramecium bursariapotentially a good model for(secondary photosynthetic)
endosymbiosis?
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Broad diversity of plastid endosymbioses
Reproduced from [Arc09].
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Paramecium bursaria and its green algal endosymbionts
▶ 100 µm to 160 µm serial phagotrophic ciliate (nucleardimorphism).
▶ ∼ 300 endosymbiotic algae in stable heritable facultative(?)endosymbiosis.
▶ Multiple independent origins of these endosymbioses.▶ Single cell transcriptome and genome of P.
bursaria-Micractinium reisseri CCAP 1660/12.▶ P. bursaria bulk transcriptome Yad1g1N [KSD+14].
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RNAi pathways in the ciliates
Tetrahymena thermophila
Oxytricha trifallax
Paramecium bursaria
Paramecium caudatum
Paramecium multimicronucleatum
Paramecium sexaurelia
Paramecium primaurelia
Paramecium octaurelia
Paramecium tredecaurelia
Paramecium tetaurelia
Paramecium aureliaspecies complex
?
A good model needs a means to test hypotheses:▶ Ciliate specific scnRNA system [MG04, CMM13].▶ siRNA pathways present in Paramecium tetaurelia
[GS01, GS02] (and Tetrahymena thermophila [CL06, YC05]):1. Transgene inducible pathway [GS01].2. Exogenous dsRNA inducible pathway (feeding or injection)
[GS02].
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Transgene pathway
▶ Microinjection and transformation of MAC with high-copytransgenes lacking 3’ UTR [GS01]:
1. 23nt siRNA generated from transgene transcripts (Dcr1, Rdr2,Rdr3 and Cid2) [LNS+09, MCT+14].
2. mRNA cleavage (Ptiwi13 and Ptiwi14).
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Exogenous dsRNA pathway
Reproduced from [CGA+15].
▶ Exogenous dsRNA viafeeding (or microinjection)[GS02].
▶ 1◦ siRNA targeted cleavage(Ptiwi13) [BGK+11].
▶ Undefined role in MAC for2◦ siRNA (Ptiwi12, Ptiwi15)[MCT+14, CGA+15,BGK+11].
▶ Pds1 involved in uptake ofdsRNA from vacuole?[CGA+15].
▶ Activated at low levels byssRNA from normal foodbacteria [CGA+15].
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So, can we experimentally induceRNAi in P. bursaria?
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Experimental feeding vector
L4440
2790 bp
698
466
233
2790
2558
2325
2093
1860
1628
1396
1163
931
pBR322_origin
ORF frame 2
Ampicillin
AmpR_promoter
f1_origin
lacZ_a
T7_promoter
T7_promoterInsert
Transformed into E. coli with IPTG-inducible T7 polymerase andRNAse III deficiency.
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Construct inserts
Gene Function RNAi phenotype in P. tetaureliaepi2 Epiplasmin “Monstrous” cellsNSF Membrane fusion factor Lethal
bug22 Basal body/ciliary protein Slow swimming and death
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RNAi feeding had mixed results
0
500
1000
1500
2000C
ells
per
ml
Construct = Control Construct = bug22
0 2 4 6 8 10Day
0
500
1000
1500
2000
Cells
per
ml
Construct = epi2
0 2 4 6 8 10Day
Construct = NSF
Organism
P. tetaurelia
CCAP 1660/12
Yad1g1N
HA1
186b
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Are all the known RNAi pathwaycomponents present in the active
transcriptome(s)?
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Summary of known RNAi components
Pathway Component Functiontransgene-induced siRNA Rdr3 RdRP
Ptiwi14 Piwiboth pathways Rdr2 RdRP
Dcr1 DicerPtiwi13 PiwiCid2 Nucleotidyl transferase
exogenous dsRNA-induced siRNA Rdr1 RdRPCid1 Nucleotidyl transferase
Ptiwi12 PiwiPtiwi15 PiwiPds1 Import of dsRNA?
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Cid ancestorCid
Tetrahymena thermophila [XP̲001012854.1]
Tetrahymena thermophila [XP̲001012858.1]
Paramecium bursaria SW1 [comp3906̲seq0̲m.68533]
Paramecium bursaria SW1 [comp3906̲seq0̲m.68531]
Paramecium bursaria Yad1g [TR17851̲c0̲g1̲i8̲m.235761]
Paramecium bursaria Yad1g [TR432̲c1̲g1̲i2̲m.4057]
80.7%/0.93
Paramecium biaurelia [PBIGNP33303]
Paramecium tetaurelia Cid3 [GSPATP00025353001]
Paramecium sexaurelia [PSEXPNG26288]
89.8%/0.94
Paramecium multimicronucleatum [PMMNP07604]
99.8%/1.00
Paramecium caudatum [PCAUDP10462]
91%/0.93
Paramecium tetaurelia Cid1 (Marker, 2014) [PTETP9100013001]
Paramecium biaurelia [PBIGNP26212]
Paramecium primaurelia [PPRIMP23072]
5%/0.51
Paramecium sexaurelia [PSEXPNG26738]
42%/0.71
Paramecium multimicronucleatum [PMMNP02964]
98.9%/0.99
Paramecium caudatum [PCAUDP15935]
55.4%/0.63
99.7%/1.00
59.5%/0.67
100%/1.00
97.9%/1.00
Paramecium caudatum [PSEXPNG26858]
Paramecium multimicronucleatum [PMMNP03007]
Paramecium sexaurelia [PSEXPNG26858]
Paramecium primaurelia [PPRIMP27560]
Paramecium biaurelia [PBIGNP11073]
Paramecium tetaurelia Cid2 (Marker, 2014) [PTETP13400003001]84.1%/0.91
83%/0.88
95.3%/0.96
83.9%/0.88
59.1%/0.54
99.7%/1.00
86.7%/0.69
100%/1.00
0.2
Cid2
Cid1
Cid3
Cid1-3Ancestor?
Tetrahymena thermophila
Oxytricha trifallax
Paramecium bursaria
Paramecium caudatum
Paramecium multimicronucleatum
Paramecium sexaurelia
Paramecium primaurelia
Paramecium octaurelia
Paramecium tredecaurelia
Paramecium tetaurelia
?
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Pds1 absent
Pds1
0.2
Paramecium caudatum [PCAUDP0810]
Paramecium sexaurelia [PSEXPNG04218]
Paramecium tetaurelia Psd1 (Marker, 2014) [PTETP600032001]
Paramecium primaurelia [PPRIMP00625]
Paramecium multimicronucleatum [PMMNP02700]
Paramecium biaurelia [PBIGNP01684]
Paramecium multimicronucleatum [PMMNP02686]
100%/1.0
99.5%/0.99
31.2%/0.64
87.2%/0.93
Tetrahymena thermophila
Oxytricha trifallax
Paramecium bursaria
Paramecium caudatum
Paramecium multimicronucleatum
Paramecium sexaurelia
Paramecium primaurelia
Paramecium octaurelia
Paramecium tredecaurelia
Paramecium tetaurelia
?
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Presence/absence of known pathway components
Paramecium sexaurelia
Paramecium biaurelia
Paramecium caudatum
Paramecium multimicronucleatum
Paramecium primaurelia
Paramecium tetaurelia
Paramecium bursaria
Rdr3
Rdr2
Ptiwi14
Cid1Rdr1Pds1
Ptiwi12
Ptiwi15
Presence of homologue
Absence of homologue
Unresolved
Putative unduplicated ancestral orthologue
Ptiwi13
Dcr1
Cid2
Exogenous dsRNA factors
Transgene dsRNA
Required by both
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Putative RNAi component evolution scenario
Duplication of Cid1-Cid3 ancestor into Cid1 and Cid3
Duplication of ancestral Rdr into Rdr1 and Rdr2
Whole genome duplication
AB?C
Duplication of ancestral Cid into Cid2 and Cid1-Cid3 ancestor
Tetrahymena thermophila
Oxytricha trifallax
Paramecium bursaria
Paramecium caudatum
Paramecium multimicronucleatum
Paramecium sexaurelia
Paramecium primaureliaParamecium tetaurelia
Paramecium aureliaspecies complex
? Paramecium biaurelia
Dcr1Ancestral RdrAncestral Cid
Ancestral PiwisB?A
Rdr3
Pds1B? C
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Could having a eukaryoticendosymbiont and RNAi activated bydsRNA in vacuoles be deleterious?
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Higher level of collisions with eukaryotes
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Collisions are a function of transcriptome size
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Conclusions
▶ RNAi phenotypes not inducible in most P. bursaria strains viafeeding.
▶ P. bursaria lacks Pds1 (in active transcriptome) thus may beunable to take up RNA from digestive vacuoles.
▶ High levels of 23-mer collisions between P. bursaria andeukaryotic endosymbiont transcriptomes may lead todeactivation of dsRNA uptake from vacuoles.
▶ Presence of other factors in active transcriptomes of P.bursaria indicate transgene and microinjected exogenousdsRNA pathways may function.
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Acknowledgements
▶ Ben Jenkins (feeding experiments)▶ David Milner (labwork)▶ Tom Richards (PI)▶ NHM-UCL PhD Studentship (main funding)
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References[Arc09] John M Archibald. The puzzle of plastid evolution. Curr. Biol., 19(2):R81–8, January 2009.
[BGK+11] K. Bouhouche, J.-F. Gout, a. Kapusta, M. Betermier, and E. Meyer. Functionalspecialization of Piwi proteins in Paramecium tetraurelia from post-transcriptional genesilencing to genome remodelling. Nucleic Acids Res., 39(10):4249–4264, 2011.
[CGA+15] Q. Carradec, U. Gotz, O. Arnaiz, J. Pouch, M. Simon, E. Meyer, and S. Marker. Primaryand secondary siRNA synthesis triggered by RNAs from food bacteria in the ciliateParamecium tetraurelia. Nucleic Acids Res., 43(3):1818–1833, 2015.
[CL06] Kathleen Collins and Suzanne R Lee. Two classes of endogenous small RNAs inTetrahymena thermophila. Genes Dev., 20:28–33, 2006.
[CMM13] Douglas L Chalker, E. Meyer, and Kazufumi Mochizuki. Epigenetics of ciliates. Cold SpringHarb. Prespectives Epigenetics, 5:a017764, 2013.
[GS01] Angélique Galvani and Linda Sperling. Transgene-mediated post-transcriptional genesilencing is inhibited by 3 non-coding sequences in Paramecium. Nucleic Acids Res.,29(21):4387–4394, 2001.
[GS02] Angélique Galvani and Linda Sperling. RNA interference by feeding in Paramecium. TrendsGenet., 18(1):11–2, January 2002.
[KSD+14] Yuuki Kodama, Haruo Suzuki, Hideo Dohra, Manabu Sugii, Tatsuya Kitazume, KatsushiYamaguchi, Shuji Shigenobu, and Masahiro Fujishima. Comparison of gene expression ofParamecium bursaria with and without Chlorella variabilis symbionts. BMC Genomics,15:183, 2014.
[LNS+09] G. Lepere, M. Nowacki, V. Serrano, J.-F. Gout, G. Guglielmi, S. Duharcourt, and E. Meyer.Silencing-associated and meiosis-specific small RNA pathways in Paramecium tetraurelia.Nucleic Acids Res., 37(3):903–915, 2009.
[MCT+14] Simone Marker, Quentin Carradec, Véronique Tanty, Olivier Arnaiz, and Eric Meyer. Aforward genetic screen reveals essential and non-essential RNAi factors in Parameciumtetraurelia. Nucleic Acids Res., 42(11):7268–7280, 2014.
[MG04] Kazufumi Mochizuki and Martin a. Gorovsky. Conjugation-specific small RNAs inTetrahymena have predicted properties of scan (scn) RNAs involved in genomerearrangement. Genes Dev., 18(Nanney 1974):2068–2073, 2004.
[YC05] Meng-Chao Yao and Ju-Lan Chao. RNA-guided DNA deletion in Tetrahymena: anRNAi-based mechanism for programmed genome rearrangements. Annu. Rev. Genet.,39:537–59, 2005.
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Microinjection proved difficult
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Dcr1
Paramecium bursaria SW1 [comp4263_seq4_m.73243]
Paramecium bursaria Yad1g [TR10970_c0_g1_i3_m.150314]
Paramecium multimicronucleatum [PMMNP06401]
Paramecium caudatum [PCAUDP17596]
Paramecium sexaurelia [PSEXPNG07454]
Paramecium primaurelia [PPRIMP21835]
Paramecium tetaurelia Dcr1 (Marker, 2014) [GSPATP00021751001]
Paramecium biaurelia [PBIGNP07629]
14.2%/0.41
100%/0.99
100%/1.00
30.8/50
100%/1.00
100%/1.00
Oxytricha trifallax [Contig17740.0.g110_protein]
Tetrahymena thermophila [gi_50897085_dbj_BAD34723.1]
100%/1.00
0.5Dcr1
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Rdr1 Rdr2
Rdr1 & Rdr2
Tetrahymena thermophila [XP_001026321.2]
Oxytricha trifallax [gi403346586]
Paramecium bursaria Yad1g [TR10997_c0_g2_i5_m.152701]
Paramecium bursaria SW1 [comp1394_seq3_m.27654]
Paramecium bursaria Yad1g [TR24504_c0_g1_i1_m.344314]
Paramecium bursaria SW1 [comp2093_seq0_m.40761]
Paramecium biaurelia [PBIGNP22852]
Paramecium primaurelia [PPRIMP16453]
Paramecium tetaurelia Rdr2 (Marker, 2014) [GSPATP00036857001]80.4%/0.82
Paramecium sexaurelia [PSEXPNG32890]
99.4%/0.97
Paramecium sexaurelia [PSEXPNG31119]
87.6%/0.83
Paramecium multimicronucleatum [PMMNP14096]
100%/1.00
Paramecium caudatum [PCAUDP11670]
93.3%/0.94
100%/1.00
100%/1.00
Paramecium biaurelia [PBIGNP28717]
Paramecium tetaurelia Rdr1 (Marker, 2014) [PTETP8500012001]
Paramecium primaurelia [PPRIMP21923]
64.1%/0.81
Paramecium sexaurelia [PSEXPNG23839]
98.5%/0.98
96.7%/0.99
Paramecium caudatum [PCAUDP07869]
Paramecium multimicronucleatum [PMMNP15732]24.6%/0.5
99.9%/1.00
100%/1.00
100%/1.00
76.7%/0.85
99.2%
0.2
/0.99
Rdr1
Rdr2
Rdr1?
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Rdr3
Paramecium bursaria Yad1g [TR463_c0_g2_i1_m.5849]
Paramecium bursaria SW1 [comp11757_seq0_m.132993]
Paramecium biaurelia [BIGNP03905]
Paramecium primaurelia [PPRIMP22734]
Paramecium tetaurelia Rdr3 (Marker, 2014) [GSPATP00006401001]
17.7%/0.50
Paramecium sexaurelia [PSEXPNG06491]
98.4%/0.96
Paramecium multimicronucleatum [PMMNP00936]
100%/1.00
Paramecium caudatum [PCAUDP10550]
96.6%/0.92
100%/1.00
0.2Rdr3
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Psd1 Structure