Rick Yost

Mass Spectrometry and Ion Mobility Spectrometry

Analytical ChemistryUniversity of Florida

Other UF Research with ES CSTC and

Space Biotechnology Applications

UF Facts

UF offers more academic programs on one campus than any other University (tied with Ohio State and Minnesota)

43,500 students on campus, the 6th largest university in US - (10,000 grad students, 3,000 professional, 31,500 undergrads)

4,000 faculty $300M in research grants/year 8th among all universities in royalty income 8th among all universities in number of U.S. patents awarded

among the top 8 PhD-granting Chemistry departments in the US 12th in total research spending for Chemistry 1st in industrial funding for Chemistry

46 tenured or tenure-track faculty 7 teaching and research support faculty 250 graduate students 85 postdoctoral and other scientific associates 42 staff members

UF Chemistry Facts

Department of Chemistry

http://www.acs.org/education/cpt/cptsr01.html

UF

UF Graduate Program in Chemistry

among the top four Analytical Chemistry programs in the US

8 tenured faculty 2 teaching and research support faculty 81 graduate students 20 postdoctoral associates

Analytical Chemistry

UF Analytical Chemistry Facts

Analytical chemistry Instrumentation development and applications High-performance mass spectrometry High-field ion mobility spectrometry (FAIMS)

Over $5M in grants ($1.2M from NASA)Close collaboration with industrial partnersOver $2B worth of analytical instruments sold based upon these partnerships  

Yost Research Group

Completed Projects

NASA-Ames/KSC, Finnigan - Mass Spectrometric Automation and Artificial Intelligence for NASA Manned Space Environments

NASA/Dynacs - Design of a Miniature Ion Trap Mass Spectrometer for Launchpad Monitoring

FSGC/Dynamac - Miniature Mass Spectrometer for Biological and Environmental Monitoring

FSGC - Laser Microprobe Mass Spectrometry for Mapping Compounds in Intact Tissue

NASA Projects

Pending Projects

UF/ES CSTC/Stennis – New Analytical Methods and Instrumentation for Monitoring Plant Stress

UF/SABRE – Monitoring Plant Stress for Plants Grown under Martian Atmospheric Conditions

UF/SABRE – Metabolomics (Metabolic Profiling) of Plant Stress by LC/MS/MS

UF/UCF-Development of a Virtual Range to Validate Safety Criteria of Current and 2nd and 3rd Generation Space Vehicles

(Fieldable Sensors)

NASA Projects

Technology for Environmental Science and Biotechnology Applications

High-performance mass spectrometryTandem mass spectrometry (MS/MS)Laser microprobe MS/MSFourier-transform mass spectrometry (FTMS)

High-field ion mobility spectrometry (FAIMS)programmable multi-component sensors (small, low-power)remarkably high sensitivity and selectivity similar to IMS systems used for explosives detectionsimilar to the IMS system (VOA) on board the International Space Station

vs. Tandem-in-Time

(Quadrupole Ion Trap)

(Triple Quadrupole)

Tandem-in-Space

Tandem mass spectrometry (MS/MS)

Tandem mass spectrometry (MS/MS)

Enhance selectivity by combining complimentary techniques

Number of Stages =

1. GC

2. GC/MS

3. GC/MS/MS

4. GC/MS/MS/MS 1 2 3 4

Number of Stages of Analysis

Signal

Chemical Noise

S/N

Laser microprobe MS/MS

Laser

Ion Trap

Sample Probe

Ion Source

L D / C I - E x p e r i m e n t a l D r u g I n t e n s i t y i n T i s s u e

0

5 0 0 0

1 0 0 0 0

1 5 0 0 0

2 0 0 0 0

2 5 0 0 0

3 0 0 0 0

3 5 0 0 0

0 1 2 3 4D i s t a n c e ( m m )

Intensit

y ( arb. unit

s)

Map trace compounds at low ppm levels in intact tissue – drugs, metabolites, biomarkers

Spatial resolution 100μm, potentially 10μm

Fourier-Transform mass spectrometry (FTMS)

Ultra-high resolution (105 @ m/z 100)

Exact mass for empirical formulae

MS/MS for structure elucidation

1/10 the size and weight of conventional FTMS systems

R ~ 10,000 @ m/z 28

Ion Mobility Spectrometry

Conventional IMS

To Detector

FAIMS apparatus

Measure size/charge of ions at atmospheric pressure

IMS widely used for explosives, chemical agent detection, VOA on ISSS

FAIMS offers far higher sensitivity and selectivity

FAIMS has potential for miniaturization

High-field Ion Mobility Spectrometry (FAIMS)

Laser microprobe MS/MSThermo Finnigan

Fourier-transform MSSiemens Automation

High-field ion mobility spectrometry (FAIMS)Ionalytics, a spin-off from NRC Canada Sionex, a spin-off fromCharles Stark Draper Lab at MIT

Applications (and/or instrumentation)UF, NASA research centersNorth Carolina partners

Projected Partnerships