Research Training in Immunology at Brazilian Laboratory
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Research Training in Immunology at Brazilian Laboratory Conclusion References Acknowledgements Introduction Materials and Methods Objective Results The purpose of this study was to evaluate the anti-inflammatory property of warifteine, an alkaloid of Cissampelos sympodialis, a medicinal plant used in Brazil to treat airway inflammatory processes. The results demonstrated that the compound Warifeine modulates the inflammatory process by decreasing the edema formation induced by phlogistic agents. These results will allow us to investigate more this plant and its compounds in order to prove that Cissampelos sympodialis can be used as an alternative medicine to treat inflammatory processes including allergic diseases. Al Ali, E ; Alves, A.F.; Pacheco-Oliveira, M.; T. Muccini; Ramalho, T.R.; Costa, H.F.; Izidro- Bezerra, A.; Alves, A.K. A.; Paiva-Ferreira, L.K.D.; Vieira, G.C. and Piuvezam, M.R. [email protected] / [email protected] Universidade Federal da Paraiba (UFPB) Programa de Pos-Graduacao em Produtos Naturais e Sinteticos Bioativos (PgPNSB/UFPB) Immunology is the science that study the recognition of aggressors and how the body defends itself from the action of these antigens. The Immunopharmacology Laboratory of the Federal University of Paraíba (UFPB) carries several lines of research, such as inflammation and hypersensitivity. Inflammation is a body response against harmful agents, which is characterized by vasodilatation and fluids flow into the inflamed tissues. These events are mediated by various chemical agents for example PGE 2 , bradykinin and serotonin and leukocyte migration into the inflamed tissue. The cells migrate to the endothelium by attaching to selectin and integrin molecules on the endothelium cells. Finally, when the leukocytes reach the inflamed tissue they perform their functions and the inflammatory process may end or evolve to chronicity. Hypersensitivity is an exaggerated response of the body against antigens. The process started by the exposure to antigen which lead to the activation of Th2 cells and B cells and IgE production that will bind to Fc receptor of mast cell. The reexpouser to the antigen will create a pathologic reaction. Warifteine (2.0 mg/kg or 10.0 mg/gh)) Saline PGE 2 , bradikinin or carrageenan 1 hour Indomethacine Saline carrageenan Paw edema PGE 2 bradikinin BARBOSA-FILHO, et al. Ciência e Cultura, 49, 386-94, 1997. PIUVEZAN et al. J. Ethnopharmacol , 67, 93-101, 1999 BEZERRA-SANTOS et al. Int. Immunopharmacol., 6 (7): 1152-60, 2006. COSTA et al. Int. Immunopharmacol , 8, 519-25, 2008. CERQUEIRA-LIMA et al. Current Drug Targets, 11, 2010 VIEIRA, G. C., 2012. Phytoterapy Research (accepted) ABBAS, et Al, (2007),Cellular and Molecular Immunology, 6 th Edition, Saunders Elsever.
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Research Training in Immunology at Brazilian Laboratory. Al Ali, E ; Alves, A.F.; Pacheco-Oliveira, M.; T. Muccini; Ramalho , T.R.; Costa, H.F.; Izidro-Bezerra, A.; Alves, A.K. A.; Paiva -Ferreira, L.K.D.; Vieira, G.C. and Piuvezam, M.R. [email protected] / [email protected] - PowerPoint PPT Presentation
Transcript of Research Training in Immunology at Brazilian Laboratory
Research Training in Immunology at Brazilian Laboratory
Conclusion
References Acknowledgements
Introduction
Materials and Methods
Objective
Results
The purpose of this study was to evaluate the anti-inflammatory property of warifteine, an alkaloid of Cissampelos sympodialis, a medicinal plant used in Brazil to treat airway inflammatory processes.
The results demonstrated that the compound Warifeine modulates the inflammatory process by decreasing the edema formation induced by phlogistic agents. These results will allow us to investigate more this plant and its compounds in order to prove that Cissampelos sympodialis can be used as an alternative
medicine to treat inflammatory processes including allergic diseases.
Al Ali, E ; Alves, A.F.; Pacheco-Oliveira, M.; T. Muccini; Ramalho, T.R.; Costa, H.F.; Izidro-Bezerra, A.; Alves, A.K. A.; Paiva-Ferreira, L.K.D.; Vieira, G.C. and
Piuvezam, M.R.
[email protected] / [email protected] Universidade Federal da Paraiba (UFPB)Programa de Pos-Graduacao em Produtos Naturais e Sinteticos Bioativos (PgPNSB/UFPB)
Immunology is the science that study the recognition of aggressors and how the body defends itself from the action of these antigens. The Immunopharmacology Laboratory of the Federal University of Paraíba (UFPB) carries several lines of research, such as inflammation and hypersensitivity.
Inflammation is a body response against harmful agents, which is characterized by vasodilatation and fluids flow into the inflamed tissues. These events are mediated by various chemical agents for example PGE2, bradykinin and serotonin and leukocyte migration into the inflamed tissue. The cells migrate to the endothelium by attaching to selectin and integrin molecules on the endothelium cells. Finally, when the leukocytes reach the inflamed tissue they perform their functions and the inflammatory process may end or evolve to chronicity.
Hypersensitivity is an exaggerated response of the body against antigens. The process started by the exposure to antigen which lead to the activation of Th2 cells and B cells and IgE production that will bind to Fc receptor of mast cell. The reexpouser to the antigen will create a pathologic reaction.
Warifteine (2.0 mg/kg or 10.0
mg/gh))
Saline
PGE2 , bradikinin or carrageenan
1 hour
Indomethacine
Saline
carrageenan
Paw edema
PGE2
bradikinin
BARBOSA-FILHO, et al. Ciência e Cultura, 49, 386-94, 1997.PIUVEZAN et al. J. Ethnopharmacol, 67, 93-101, 1999BEZERRA-SANTOS et al. Int. Immunopharmacol., 6 (7): 1152-60, 2006.COSTA et al. Int. Immunopharmacol, 8, 519-25, 2008.CERQUEIRA-LIMA et al. Current Drug Targets, 11, 2010VIEIRA, G. C., 2012. Phytoterapy Research (accepted)ABBAS, et Al, (2007),Cellular and Molecular Immunology, 6th Edition, Saunders Elsever.
