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Opportunities for cooperation between ChinAquaNet and Ghent University
Ghent, August 31, 2007
UGENT AQUACULTURE R&D CENTRE
Research at the Lab. of Aquaculture& Artemia Reference Center
Research at the Lab. of Aquaculture& Artemia Reference Center
Peter BossierPatrick Sorgeloos
Peter BossierPatrick Sorgeloos
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 2 of 22
Artemia research
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 3 of 22
A. persimilis
A. franciscana monica
A. urmiana
A. sp. (unidentified population)A. parthenogeneticaA. franciscanaA. salinaA. sinica
A. tibetiana
World distribution of Artemia species• bisexual species : A. salina, A. urmiana, A. sinica, A. tibetiana, A. franciscana,
A. persimilis• parthenogenetic populations
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 4 of 22
established in 1978 upon suggestion of the FAO
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
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Genetic fingerprinting patterns (RFLP)for Artemia species authentication
RFLP HpaII
100 bp DNA ladder
A. salina
A. persimilis
parthenogenetic Art.
parthenogenetic Art.
A.franciscana
A. franciscana
A. franciscana
A. franciscana
A. franciscana
A. sinica
A. sinica
Mégrine, Tunisia
Argentina*
Aibi Lake, Xinjiang, China
Vineta Swakopmund, Namibia
Vinh Chau, Vietnam
San Francisco Bay, California, USA
Vinh Chau, Vietnam
Macau, Brazil
Great Salt Lake, USA
Xiechi Lake, Yuncheng, Shanxi, China
Yimeng area, Inner Mongolia, China*
1268
1321
1236
1186
1301
1258
1456
1300
1287
1218
1188
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 6 of 22
Developing new tools for Artemia population studies:
•In a lot of Artemia site, populations seem to contain more than one species or strain
•Commercial products can contain Artemia cysts from different origin
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 7 of 22
Bohai bay, China
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 8 of 22
DGGE on Artemia from Bohai Bay:
studying mixed species/strain populations
parth A. sinica
In each lane, each band represent a different rDNA genotype
A. franciscana
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 9 of 22
DGGE on Artemia:comparison with individual analysis
franciscana
parth
ARCcode
A. parth%
A. sinica
%
A. franc%
97936891
1001418979300
?
11251211161712191187161612401213123312611350
00060803900
10097
3732300253700
000006180003
sinica
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 10 of 22
Nutritional research
Artemia enrichment
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 11 of 22
10-day old seabass larva feeding on Artemia
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
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pigments
prophylactics
essential nutrients
Bioencapsulation or Enrichment
therapeutics
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 13 of 22
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 14 of 22
Lipid emulsion in gut of Artemia
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 15 of 22
Nutritional research
Brachionus genetic diversity
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 16 of 22
Follow up of culture H
00.10.20.30.40.50.60.70.80.9
1
H0 H5 H8 H10 H12 H14 H16auscay
DGGE for studying mixtures of cryptic Brachionus species
Austria Cayman
H0 H5 H8 H10 H12 H14 H16
RFLP on individuals
DGGE on mixtures
17 consecutive batches (3 days )of Brachionus culturing on industrial scale
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
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Larviculture Research
Mud crab : Scylla spp.
Macrobrachium rosenbergii
Penaeid shrimp
Turbot
Sea bass
cobia
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 18 of 22
Host-microbial interactions
Quorum sensing
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 19 of 22
Quorum sensing in Vibrio harveyi
QUORUM SENSING (QS) = Bacterial cell-to-cellcommunication with signal molecules
LuxSLuxQ
LuxP
LuxM
LuxN
HAI-1 AI-2CAI-1
CqsS
CqsALuxU
LuxO
LuxR
sRNA’s + Hfq
Target genes
σ54
N O
OH
H OH O
B-
OH
OH
OH OH
HAI-1= AHL
AI-2
furanosyl borate diester
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
slide 20 of 22
Control
Wildtype
HAI-1 synthase
HAI-1 receptor
AI-2 synthase
AI-2 receptor
HAI-1 & AI-2 synthaseLuxO
Surv
ival
(%)
0
20
40
60
80
100
Artemia challenge with QS MUTANTS of Vibrio BB120
**
*
*: significantly different from control (P < 0,01)
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
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Effect of AHL on Turbot larvaesurvival in % on DAH7
Factor AHL addition
- +
-
(Treatment 1)
92.1 ± 8.3bc
(Treatment 2)
10.4 ± 10.0a
EC addition
EC3
(Treatment 3)
62.1 ± 24.4b
(Treatment 4)
2.1 ± 3.5a
EC5
(Treatment 5)
96.7 ± 4.0c
(Treatment 6)
94.3 ± 7.1bc
Research at the Lab. of Aquaculture & Artemia Reference CenterUGent Aquaculture R&D Centre
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Effect of AHL onMacrobrachium larviculture
Survival rate on day 8
77.7c77.1c77.3c
61.5bc
48.5ab
37.3a
31323334353637383
AHL+EC5 EC5 AHL+LVS3 LVS3 AHL Control
Treatment
Surv
ival
rate
(%)