REPORT: STATUS OF FRACTURE CRITICAL TRUSS BRIDGES
Transcript of REPORT: STATUS OF FRACTURE CRITICAL TRUSS BRIDGES
©2008 Waters Corporation 1
Productos DisQuETM
para la preparación de muestra porQUECHERS
©2008 Waters Corporation 2
ReminderDispersive Sample Preparation“QuEChERS” Method
“Quick, Easy, Cheap, Effective, Rugged, Safe”
Where— Food laboratories
Application— High throughput sample preparation for pesticide screening analysis
Technique— Combination of salting-out LLE and matrix dispersion extraction
©2008 Waters Corporation 3
Dispersive Sample PreparationProduct offerings
PN 186004571
PN 186004572
PN 186004837
PN 186004832
Product Qty P/N
DisQuE Kits
DisQuE AOAC Dispersive Sample Preparation Kit 100 176001676
DisQuE CEN Dispersive Sample Preparation Kit 100 176001903
Individual Extraction tubes (Tube 1)
DisQuE 50 ml tube/AOAC - Acetate (1,5 g Sodium Acetate/6g MgSO4) 100 186004571
DisQuE 50 ml tube/CEN - Citrate (1,5 g Sodium Citrate/1g NaCl/4g MgSO4) 100 186004837
In dividual Clean-up tubes (tube 2)
DisQuE 2 ml tube - AOAC (150 mg MgSO4/50 mg PSA) 100 186004572
DisQuE 2 ml tube - AOAC/C18 (150 mg MgSO4/50 mg PSA/50mg C18) 100 186004830
DisQuE 2 ml tube - CEN (150 mg MgSO4/25 mg PSA) 100 186004831
DisQuE 2 ml tube - CEN/C18 (150 mg MgSO4/25 mg PSA/25mg C18) 100 186004832
DisQuE 15 ml tube / MgSO4/PSA (900 mg MgSO4/150 mg PSA) 50 186004833
DisQuE 15 ml tube / MgSO4/PSA/C18 (900 mg MgSO4/150 mg PSA/150 mg C18) 50 186004834
Bulk sorbents
Graphitized Carbon Black 25 g bottle 1 186004835
C18 100g bottle 1 WAT035672
©2008 Waters Corporation 4
DisQuE Kits
AOAC (Association of Analytical Communities )— worldwide provider and facilitator in the development, use, and
harmonization of validated analytical methods and laboratory quality assurance programs and services (primarily used in the US but European laboratories are considering their recommendations as well).
PN 176001676: DisQuE AOAC Dispersive Sample Preparation Kit
Description— DisQuE Tube 1 (Extraction):
o 50 mL centrifuge tubeo 1.5 g anhydrous sodium acetateo 6 g of anhydrous magnesium sulphate
— DisQuE Tube 2 (Clean-up):o 2 mL centrifuge tubeo 150 mg anhydrous magnesium sulphateo 50 mg of PSA
©2008 Waters Corporation 5
DisQuE ProcedureAOAC Method
Prepare Sample
Homogenize15 g sample
15 mL 1% Acetic Acid in ACN
Liquid FractionationShake for 1 minute
Centrifuge >1500 x g
CollectionRemove acetonitrile for clean-up
DisQuE ExtractionTube 1
Transfer1 mL Extract
Tube 1 to Tube 2
Shake vigously for 1 minuteCentrifuge >1500 x g
TransferCollect to autosampler vial
Dilute if necessary
DisQuE Clean-UpTube 2
©2008 Waters Corporation 6
DisQuE™ AOAC Application note
DisQuE extraction tested against 400 pesticide residue method
Matrix — Avocado, Baby food & Mango
Excellent recovery
Consistent results
©2008 Waters Corporation 7
DisQuE Kits
CEN (European Committee for Standardization )— business facilitator in Europe, removing trade barriers for European
industry and consumers. It provides a platform for the development of European Standards and other technical specifications.
PN 176001903: DisQuE CEN Dispersive Sample Preparation Kit
The European Norm has the number EN 15662:2008
Title:— Foods of plant origin - Determination of pesticide residues using GC-MS
and/or LC-MS/MS following acetonitrile extraction/partitioning and clean-up by dispersive SPE - QuEChERS-method
©2008 Waters Corporation 8
European harmonization of EN 15662:2008
The European Norm is accepted and adapted by the national “authorities” and a national document is produced.
Below you will find the national organizations and the reference for the national documents corresponding to the EN 15662:2008 norm
Country National Organization National Document ReferenceNetherlands NEN NEN-EN 15662:2008Germany DIN DIN EN 15662Switzerland SNV SN EN 15662:2009France AFNOR NF EN 15662Italy UNI UNI EN 15662:2009Sweden SIS SS-EN 15662:2008Norway SN NS-EN 15662:2008Finland SFS SFS-EN 15662:enIceland IST ÍST EN 15662:2008Estonia EVS EVS-EN 15662:2008Ireland NSAI I.S. EN 15662:2008
Country National Organization National Document ReferenceAustria ON OENORM EN 15662Hungary MSZT MSZ EN 15662:2009Poland PKN PN-EN 15662:2008 (U)Slovenia SIST SIST EN 15662:2009Croatia HZN HRN EN 15662:2008Lithuania LST LST EN 15662:2009Romania ASRO SR EN 15662:2009Malta MSA MSA EN 15662:2005Latvia LVS LVS EN 15662:2009
©2008 Waters Corporation 9
DisQuE ProcedureCEN Method
PN 176001903: DisQuE CEN Dispersive Sample Preparation Kit
Description— DisQuE Tube 1 (Extraction):
o 50 mL centrifuge tubeo 4 g magnesium sulfate anhydrouso 1 g sodium chlorideo 1 g trisodium citrate dihydrateo 0.5 g disodium hydrogencitrate
— DisQuE Tube 2 (Clean-up):o 2 mL centrifuge tubeo 150 mg anhydrous magnesium sulphateo 25 mg of PSAo 25 mg C18
©2008 Waters Corporation 10
DisQuE ProcedureCEN Method
Prepare Sample
Homogenize10 g sample* 10 mL ACN**
Liquid FractionationShake for 1 minute
Centrifuge 5 min>3000 g
CollectionRemove acetonitrile for clean-up
DisQuE ExtractionTube 1
Transfer1 mL Extract
Tube 1 to Tube 2
Shake vigously for 1 minuteCentrifuge >1500 x g
TransferCollect to autosampler vial
Dilute if necessary
DisQuE Clean-UpTube 2
*Depending on the matrices
**If highly acidic compounds add NaOH
©2008 Waters Corporation 11
DisQuE ProcedureCEN Method
The reason why the CEN norm came out was that the ACN extract made with the AOAC method was not clean enough. A cleaner extract is obtained with the citrate salt combination.
In our CEN kit tube 2 includes C18 (option with only PSA is available)
— C18 will remove co-extracted fat and waxes often present in these matrices. Their present affects the ruggedness of the further analysis.
— Graphitized Carbon Black is used if the samples has a high content of carotinoides (e.g. red pepper, corrots) or chlorophyll (e.g. spinach, rucola salad, vine leaves)
©2008 Waters Corporation 12
DisQuE Overview
The DisQuE 15 ml tube 2 is available for customers who wishes to use 6 ml of the ACN phase from tube 1. two version exist:— CEN PN 186004833 DisQuE 15 ml tube/MgSO4/PSA/C18— AOAC PN 186004834 DisQuE 15 ml tube/MgSO4/PSA