Report 0218-2008: Steryl Glucoside Calibration

By Haiying Tang

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Steryl glucoside(SG)

ISTD1

ISTD2

GC-FID Chromatography: Steryl glucoside (Standard,Matreya)

GC-FID Chromatography: Sterol glycoside (Standard,Matreya)

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18.0 18.2 18.4 18.6 18.8 19.0 19.2 19.4 19.6 19.8 20.0 20.2 20.4 20.6 20.8 21.0 21.2 21.4 21.6 21.8

ISTD2

SG

GC-FID Chromatography

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STD:Mono1,3,5

STD:SG

STD: Total glycerolglycerol

ISTD1

ISTD2

Diglycerides triglyceridesISTD1

ISTD1

Mono1

Mono

Mono3,5 SG

Calibration CurveSG calibration

y = 8655.1x

R2 = 0.9974

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SG concentration (mg/l)

GC

-FID

Ch

rom

agra

ph

y A

rea

`

GC-FID Chromatography

18.2 18.4 18.6 18.8 19.0 19.2 19.4 19.6 19.8 20.0 20.2 20.4 20.6 20.8 21.0 21.2 21.4 21.6 21.8

SG-Std

REG-insoluble

ISTD2SG

18.2 18.4 18.6 18.8 19.0 19.2 19.4 19.6 19.8 20.0 20.2 20.4 20.6 20.8 21.0 21.2 21.4 21.6 21.8 22.0 22.2 22.4 22.6 22.8

STD-SG

Insoluble-B100soy

STD:Di-;Tri-

GC-FID Chromatography

Quantity of SG in Biodiesel

  AreaSG Conc.(mg/l) SG

REG 2665630 307.9861.6%(purity) 

Insoluble-SBO 91906.32 10.62 27510 ppm 

Insoluble-CSO 13304.86 1.53 1302 ppm B100-SBO(after filtration) 13016.11 1.5 104 ppmB100-CSO( after filtration) 23380.21 2.7 219 ppm

Future Work

• Insoluble formation on SBO-based biodiesel (NextDiesel)

• Quantify the SG in biodiesel using GC-FID

GC-FID Chromatography: REG

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18.2 18.4 18.6 18.8 19.0 19.2 19.4 19.6 19.8 20.0 20.2 20.4 20.6 20.8 21.0 21.2 21.4 21.6 21.8

Table 1. Sterol composition in some seed oils of commercial importance (mg/Kg).

Corn oil cottonseed olive palm rapeseed safflower soybean sunflower

cholesterol - - - 26 - - - -

campesterol 2691 170 28 358 1530 452 720 313

stigmasterol 702 42 14 204 - 313 720 313

β-sitosterol 7722 3961 1310 1894 3549 1809 1908 2352

Δ5-avenasterol 468 85 29 51 122 35 108 156

Δ7-stigmastenol 117 - 58 25 306 696 108 588

Δ7-avenasterol - - - - - 104 36 156

brassicasterol - - - - 612 - - -

other - - - - - 69 - 39

Data from Gunstone, F.D., Harwood, J.L. and Padley, F.B. The Lipid Handbook (Second Edition) (Chapman & Hall, London) (1994).