7/16/2019 Removal of hexavalent chrome from industrial effluents with the microalga Chlorella vulgaris (Chlorophyta)

http://slidepdf.com/reader/full/removal-of-hexavalent-chrome-from-industrial-effluents-with-the-microalga-chlorella 1/1

Removal of hexavalent chrome from industrial effluents

with the microalga Chlorel la vulgar is (Chlorophyta)2 Alex Dueñas-Gonza; 1Ronald Huarachi-Olivera*; 2Ursulo Yapo-Pari; 2Javier Valencia-Yucra.

1Magister Program in Biotechnology, University of Antofagasta, Antofagasta, Chile.2 Aquatic Biology Laboratory, Academic Department of Biology, National University of San Agustin, Arequipa, Peru, Av. Alcides Carrión s/n

E-mail: ronald.olivera@uantof.cl

Introduction:Chlor ella vulg aris Beyerinck [ Beijerin ck ]  1890 is a green unicellular and spherical eukaryotic microalgae. It can accumulate over 70% of Cr icell wall and has been used for studies of metabolic stress (Rai et. al., 2013 ) .

Heavy metal pollution is a global problem because of its toxicity in the human body both in the food chain as well as its carcinogenic eff

(He & Chen 2014) . Trivalent chromium is usually a non-mobile and non-toxic micronutrient ( René' et . al., 2006 ) . Cr +6 is soluble in water , is

and carcinogenic . To date there are no results on the removal of Cr +6 present in industrial effluents PIRS microalgae , so in this study , the

was to determine the ability of removal of Cr +6

in industrial effluents with microalgae C. vulg aris .

Material and Methods

The microalga C. vulgar is  was isolated and cultured in synthetic gutter (Valiente, 1995), pH = 6.5 ; to 25°  C based on a light - cool w

fluorescent illumination constant

The microalgae Chlorel la vulgar is  was obtained from samples of water from effluent treatment plant Chilpina , Arequipa - Peru ( 71°32'15,93 "

16°26'50,76 " LS ; 2293 m).

Samples were taken periodically every seven days for a period of 21 days to assess  C. vulgar is  counting using a Neubauer chamber and

microscopy at 10X.

Chromium determinations were performed by atomic absorption spectroscopy with a detection limit of 190-900 nm in a Shimad

spectrophotometer AA -6800.

Results and Discusions

Conclusions

Concentration 75% of industrial effluents by applying 50 mL inoculum of  C. vulgar is  microalgae, reached a maximum removal efficiency of 9Cr +6 to 21 days , while allowing further growth microalgae ( 2260-10293 nº organisms C. vulgar is  /mL).

Growth of  Chlorel la vulgaris  at different

concentrations of Cr +6

(K2Cr 2O7). (A) 7days, (B) 14 days and (C) 21 days.

Shows the groups "a", "ab" and "b" are

the names of each group for comparis

ons, according to Tukey test.

Growth   of   Chlorel la vu lgar is   at different

concentrations of industrial effluents.

(A) 7 days, (B) 14 days and (C) 21 days. Shows

"a", "ab" and "b" groups being denominations

of each group for comparisons according to

Tukey's test.

Evaluation of   removal   of residual Cr +6 t

concentrations of 25, 50 and 75% of industria

effluents with application of 50 mL of inoculum

of  Chlorel la vulgaris.  (A) 7 days, (B) 14 day

and (C) 21 days. Shows "a", "ab" and "b

groups being the designations of each grou

for comparisons according to Tukey's test

The initial number of cells of  C. vulg aris  was 2904 organisms/mL observing a significant increase in the number of cells after 14 day

toxicological evaluation with 174,507 organisms/mL in the control with respect to concentrations as Cr +6 K2Cr 2O7 . After 21 days , no differe

in growth between the concentrations of algae is observed.

The initial number of cells of  C. vulg aris  was 2260 organisms/mL observed after 14 days in the concentration of industrial effluents 50% hi

growth compared to the control cells , with an average of 11680 organisms/mL. This growth is not related to increased metal removal capabil

water , as described above. After 21 days it is observed that the effluent concentration of 75% was the highest estimated growth of  C. vulg

with an average of 10293 organisms/mL , this growth explain the higher metal removal.

The composition of industrial effluents Parque Industrial río Seco (PIRS), has an initial concentration of 1.24 mg/L Cr +6 being removeC. vulgaris  significantly to 21 days reaching a maximum removal efficiency of 99.2 % with 0.01 mg/L of Cr +6 concentration of 75% indu

effluents.

Microalgae Chlorel la vulgar is    Chlorel la vulgar is  countin Neubauer chamber 

Toxicological Evaluation of

Cr +6 and effluent industrialwith Chlorel la vulgar is 

Atomic absorption spectrophotomet

TREATMENT:

- 0%=0 mL effluents + 200 mL distil led water -25%= 50 mL effluents + 150 mL distilled water - 50%=100 mL effluents + 100 mL distilled water -75% = 150 mL effluents + 50 mL distilled water 

50 mL inoculum

C. vulg aris 

50 mL inocul

C. vulgaris 

i Olivera

livera@uantof.clarchgate.net/profile/Ronald_Olivera

BibliographyRAI, U., N. SINGH, A. UPADHYAY, & S. VERMA. 2013. Chromate tolerance and accumulation in  Chlo re l la vu lgaris  L.: Role of antioxidant enzymes and biochemical changes in detoxification of metals. Bioresource technology, 136, 604-609.

HE, J., & J. CHEN. 2014. A comprehensive review on biosorption of heavy metals by algal biomass: Materials, performances, chemistry, and modeling simulation tools. Bioresource Technology. Elsevier Ltd. 444 pp.

RENE' N., W. APEL, V. THOMPSON & P. SHERIDAN. 2006. Low temperature reduction of hexavalent chromium by a microbial enrichment consortium and a novel strain of Arthrobacter aurescens. BMC microbiology, 6(1), 5.

VALIENTE, V. 1995. Compendio de medios de cultivos, soluciones y extractos para microalgas y cianobacterias. CNIC. 1ra Ed. Cuba.