RDNA Activity
5
Recall: recombinant DNA technology ● The things that you need: – The gene of interest (or its source) – The vector (e.g. plasmid) – The enzyme that digests the DNA ● Restricon enzyme – The enzyme that puts the DNA fragments together ● DNA ligase
description
rdna activity
Transcript of RDNA Activity
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Recall: recombinant DNA technology
The things that you need: The gene of interest (or its source)
The vector (e.g. plasmid)
The enzyme that digests the DNA Restriction enzyme
The enzyme that puts the DNA fragments together DNA ligase
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Recall: restriction enzyme
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Group activity
Per group, construct a recombinant DNA of the Bt gene in a plasmid with promoter and terminator elements Cut the plasmid, the Bt gene,
the promoter and terminator elements with the appropriate REs
Put all the elements together to make the recombinant construct