Rapid turnover in micro- and macrobiome of free-ranging primate
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Transcript of Rapid turnover in micro- and macrobiome of free-ranging primate
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ECM7 – Stockholm, Sweden, 21.8.2015
Tuomas Aivelo
University of Helsinki
[email protected]@aivelo
Rapid turnover in micro- and macrobiome of free-ranging primate
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Microbiome research is trending
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There's lack of studies connecting microbiome and other intestinal
organisms
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Longitudinal series of parasites and microbiome very rare
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Longitudinal study How helminth and
microbiome communities differ in dynamics?
How intestinal parasites affect microbiome?
Do microbiome affect intestinal microbiome infection probability?
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Study system Rufous mouse lemur
(Microcebus rufus) Long-lived, small-sized, easy to
handle Eastern Madagascar Individuals followed for 2
(microbiome) or 3 (parasites) years
Fecal sampling: 16S sequencing microbiome, 18S sequencing nematodes, other parasites morphologically
Medlar et al. 2014 BMC Evolutionary Biology
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Nematodes
Component community stable, but infracommunity variable
Aivelo et al. 2015 Parasitology
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Nematodes
Component community stable, but infracommunity variable
Aivelo et al. 2015 Parasitology
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Significant variables
- Infracommunity: - All putative species uniformly distributed over hosts- No effect of previous infection
- Component community: - Prevalence higher in 2011 than in 2012- No effect of sex or site
Aivelo et al. 2015 Parasitology Rafalinirna et al. In press Madagascar Conservation and Development
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Significant variables
- Infracommunity: - All putative species uniformly distributed over hosts- No effect of previous infection
- Component community: - Prevalence higher in 2011 than in 2012- No effect of sex or site
Aivelo et al. 2015 Parasitology Rafalinirna et al. In press Madagascar Conservation and Development
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Microbiome
On population level stable, in individual level highly variable
20122013
Aivelo et al. in prep
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Microbiome
On population level stable, in individual level highly variable
2012
Aivelo et al. in prep
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Microbiome
On population level stable, in individual level highly variable
2013Aivelo et al. in prep
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Microbiome
On population level stable, in individual level highly variable
2013Aivelo et al. in prep
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Significant variables
Aivelo et al. in prep
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Parasite presence
Does not affect microbiome richness or diversity
EXCEPT:
20
40
60
No Eimeria EimeriaAivelo et al. in prep
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Work under progress: Interactions
Aivelo et al. in prep
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Why mouse lemurs are different?
Heterothermy? Small-size? Variation in food?
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In nutshell
Both microbiome and macrobiome vary wildly in mouse lemurs
There seems to be interactions, but...
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Random
Repeatability of 0.332
Aivelo et al. 2015 Parasitology